CLS_ACME_Catalogue

HPLC/UHPLC Columns ACME ACME PLUS ACME BIO3 ACME HPLC/UHPLC Columns 120 Å 200 Å 300 Å Cannabis 260 m2/g 200 m2/g 100 m2/g 100 Å 450 m2/g ACME C18 ACME C8 ACME PLUS ACME BIO3* ACME Cannabis ACME F5/C18 ACME Ph ACME BIO2* ACME Amide/C18 ACME CN ACME PAH ACME Biphenyl* 12 Phases *Ask us for more information on ACME Biphenyl, ACME BIO2 & ACME BIO3! RP | NP | HILIC • Leverage 3 different mechanisms of separation offered by these bonded phases to achieve optimum selectivity for all peak pairs in your sample. 4 Pore Sizes 100 Å | 120 Å | 200 Å | 300 Å • Match the stationary phase support to the molecular size of your analytes. For peptides and small proteins, choose a 200 Å pore. For molecules smaller than 5,000 Da, choose a 120 Å pore. Plus, optimize your column’s hydrophobic strength and loading capacity for your particular separation needs. 4 Particle Sizes 1.9 μm | 3 μm | 5 μm | 10 μm • Choose the right particle size for UHPLC, HPLC or preparative HPLC. 6 Standard Column Lengths 250 mm | 150 mm | 100 mm | 75 mm | 50 mm | 30 mm • Find the right balance between analysis time, resolution and peak capacity. 7 Standard Column IDs 2.1 mm | 3.0 mm | 4.6 mm | 7.8 mm | 10 mm | 21.2 mm | 30 mm Enquire for lengths or IDs not listed. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-1 LIFE SCIENCE

Colonnes HPLC/UHPLC Colonnes HPLC/UHPLC ACME silica is based on a new 120 Å ultra-high purity (99.999%) extra-treated porous spherical silica with a narrow particle size distribution and an extremely low metal content (< 10 ppm) to minimize silanol acidity and reduce surface metal sites available for chelation. Combined with our proprietary bonding technology this results in columns with optimal efficiency, asymmetry, and minimal back pressure. ACME is available in several bonded phases covering a wide range of reversed-phase applications. • Available in several particle sizes for simple method transfer between HPLC and UHPLC • Ultrapure porous silica particles enable high peak efficiency across all particle sizes • Excellent column-to-column reproducibility • Compatible with HPLC and UHPLC hardware • Consistent and reproducible retention times to allow direct scale-up from the laboratory through process applications ACME Selection Guide ACME C18 Alternate C18-like Retention with Aromatic / Olefinic Speed / Higher MW Selectivity Alternate Selectivity Isomers ACME Biphenyl ACME F5/C18 ACME PLUS • Strong aromatic selectivity • Nitrogen containing analytes • F ast C18 • Good hydrophobic retention • Geometric Isomers Applications • π-donor (Lewis base) analytes ACME Ph ACME Cannabis • MWs too large ACME Amide/C18 for ACME C18 • Aromatic analytes • Cannabinoids • p-Acidic analytes • H-bonding ACME PAH • C annabis related • Low Hydrophobicity • Polar Analytes compounds ACME C8 • PAHs ACME BIO2 • Structural isomers, ACME CN • Higher selectivity with e.g., Carotenoids • Ideal for low polar analytes using low pH applications • HILIC organic mobile phase • Low Hydrophobicity • P eptides separations ACME BIO3 • Protein separations B-2 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns HPLC/UHPLC Columns Technology through years of experience and innovation - Dr. William Campbell William Campbell received his Ph.D. in Chemistry at Montana State University under the direction of Dr. Wynn Jennings. Research included: 1. Transition metal catalysis mechanisms of Group VIII transition metals 2. Metabolism of toxic Furano-sesquiterpenes During his graduate work, Dr. Campbell gained a great deal of expertise in NMR, IR, Mass Spectroscopy and Chromatography. Dr. Campbell received a Post-Doctoral Fellowship at the University of Minnesota and continued working on organo-metallic mechanisms of Zirconocenes and Titanocenes, and organic and inorganic synthesis under the direction of Professor Paul Gassman. Dr. Campbell entered industry as a research chemist in Central Research at the Dow Corning Corporation. Through several years at Dow Corning he gained expertise in surface science and silicone and organo-silane chemistry. His love for chromatography coupled with his experience in silane chemistry lead to a career path in the development of HPLC stationary phases and applications. Early collaborations included: the Mayo Clinic, The University of Michigan Blood Chemistry Laboratory, WARS Analytical in Wisconsin and others. Dr. Campbell moved to ”Vydac” the Separations Group, where he further refined his skills in surface science, silica synthesis and evaluation, protein and peptide chromatographic techniques and development of preparative column technology. After a brief time at a startup company in Michigan, Dr. Campbell took a position at Supelco/Sigma-Aldrich as a Sr. Research Scientist and quickly moved to the position of HPLC R&D Manager. In that role he developed many new HPLC phase chemistries, presented seminars worldwide and worked closely with leading scientists at major pharmaceutical companies. Dr. Campbell is currently Adjunct Faculty in the Chemistry Department at Penn State University and the leading force behind Phase Analytical Technology. SP-180-5 PACME silica is manufactured in a GMP environment under the most rigorously controlled conditions, ensuring lot-to-lot Lot No. X170519WQP reproducibility, consistent particle size, pore volume and chemical purity. 2.000 x 5.00 µm WD: 9.5mm 3kV 2017/10/04 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-3 LIFE SCIENCE

Colonnes HPLC/UHPLCColonnes HPLC/UHPLC H = A + Bu + Cu Van Deemter Analysis Van Deemter Analysis on a Pharmaceutical Compound. ACME Silica Pore Structure shows dramatically improved performance on real analytes. H H H H H H O CH3 H H N N Cl Naphthalene Diazepam Stability Data - QC Standard Retention time reproducibility and column stability are shown on the ACME column in the figure below. The figure compares the chromatograms shown from the 1st injection compared to the 500th injection on the ACME column. The efficiency and retention factors (k) for all four test compounds are the same, a characteristic essential for long and stable column lifetimes. 2 Column: ACME C18 After 500 Injections 5 µm, 50 x 4.6 mm N = 5810 s = 0.99 Isocratic Mobile Phase: k = 2.94 Methanol:water, 80:20, (v/v) Flow rate: 1.0 mL/min Temperature: 25 °C 3 Detection: UV@265 nm 4 Injection Vol.: 3 µL Sample: 1 1. Uracil 2. Benzophenone First Injection 3. Naphthalene N = 5815 4. Biphenyl s = 0.99 k = 2.93 B-4 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Specifications C18* Cannabis F5/C18 Amide/C18 HPLC/UHPLC Columns Bonded Phase -C18H37 -C18H37 –X –-CX-1P8HF3P7 –(CH2)3NH(C=O)-C15H37 Separation Mode 260 m2/g Reversed-Phase Base Material 120 Å Purity (%) 16 Ultrapure Extra-Treated Porous Spherical Silica Surface Area ≤ 98% 99.999, metals < 10 ppm Particle Size (s) * L1 450 m2/g 260 m2/g Pore Size 120 Å Carbon Load (%) 1.9 µm, 3 µm, 5 µm Endcapping 100 Å pH Range Temperature Limit 22 16 19 Aqueous Compatibility Yes proprietary USP Listing 1.8 to 9.5 60 °C ≤ 98% 100% 100% L1/L60 L1 L1/L43 Bonded Phase PLUS* PAH C8* Ph CN** Separation Mode -C18H37 -(C18H37)x -C8H17 -R-C6H5 -R-CN Base Material Reversed-Phase Purity (%) 200 m2/g 17 Ultrapure Extra-Treated Porous Spherical Silica 260 m2/g 7 Surface Area No 99.999, metals < 10 ppm Yes 200 Å 1.8 to 9.5 120 Å proprietary Particle Size (s) * 60 °C 1.9 µm, 3 µm, 5 µm 10 2 to 7.5 Pore Size 12 Yes 50 °C Yes 10 100% Carbon Load (%) proprietary Yes proprietary L10 1.8 to 9.5 proprietary 1.8 to 9.5 Endcapping 60 °C 1.8 to 9.5 60 °C 60 °C pH Range 100% Temperature Limit ≤ 98% ≤ 98% 100% L1 Aqueous L1 L7 L11 Compatibility USP Listing * C18, C8 and PLUS 10 µm available for Prep columns **ACME CN - Separation mode also Normal Phase 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-5 LIFE SCIENCE

Colonnes HPLC/UHPLC ACME C18 Colonnes HPLC/UHPLC Physical Properties SBeopnadreadtioPnhaMseo:d-eC:18RHe3v7ersed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 260 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 120 Å Carbon Load (%): 16 Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: ≤ 98% USP Listing: L1 The ACME C18 uses high porosity silica for enhanced distribution of analytes into the phase. We use proprietary, precision bonding and end capping for good reproducibility and phase stability. • Reproducible • Large Range of Applications • High Efficiency • Wide Column Stability • Excellent Peak Shape on Basic Analytes Dimensions 1.9 μm ACME C18 - 120 Å 5 μm ID x Length (mm) ACMC18-1.9-03021 3 μm ACMC18-5-03021 2.1 x 30 ACMC18-1.9-03046 ACMC18-5-03046 4.6 x 30 ACMC18-1.9-05021 ACMC18-3-03021 ACMC18-5-05021 2.1 x 50 ACMC18-1.9-05030 ACMC18-3-03046 ACMC18-5-05030 3.0 x 50 ACMC18-1.9-05046 ACMC18-3-05021 ACMC18-5-05046 4.6 x 50 ACMC18-1.9-10021 ACMC18-3-05030 ACMC18-5-10021 2.1 x 100 ACMC18-1.9-10030 ACMC18-3-05046 ACMC18-5-10030 3.0 x 100 ACMC18-1.9-10046 ACMC18-3-10021 ACMC18-5-10046 4.6 x 100 ACMC18-1.9-15021 ACMC18-3-10030 ACMC18-5-15021 2.1 x 150 ACMC18-3-10046 ACMC18-5-15030 3.0 x 150 - ACMC18-3-15021 ACMC18-5-15046 4.6 x 150 - ACMC18-3-15030 ACMC18-5-25046 4.6 x 250 - ACMC18-3-15046 - ACME C18 - 120 Å Guard Columns Dimensions 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder ID x Length (mm) 2.1 x 5 ACMGC19UC521C18 ACMGC3LC521C18 ACMGC5LC521C18 3.0/4.6 x 5 ACMGC5LC546C18 2.1 x 10 ACMGC19UC546C18 ACMGC3LC546C18 ACMGC5LC1021C18 3.0/4.6 x 10 ACMGC5LC1046C18 ACMGC19UC1021C18 ACMGC3LC1021C18 ACMGC19UC1046C18 ACMGC3LC1046C18 Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-6 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Diazepam Metabolites ACME C18 Flow rate: 0.3 mL/min Sample: 4 HPLC/UHPLC Columns 1.9 µm, 100 x 2.1 mm Temperature: 25 °C 1. Oxazepam Isocratic Mobile Phase: Detection: UV@254 nm 2. Nordiazepam A: 0.1% formic acid in water Injection Vol.: 1 µL 3. Temazepam B: 0.1% formic acid in acetonitrile 4. Diazepam A:B, (60:40, v/v) 2 3 1. Oxazepam 2. Nordiazepam 1 IMPURITY 3. Temazepam 4. Diazepam 1.02 02.0 3.04 04.0 5.06 06.0 7.0 Time (min) Paracetamol / Aspirin Mix 1. Acetaminophen 2. Caffeine 3. Salicylamide ACME C18 4. Acetylsalicylic Acid 5. Benzoic Acid 6. Salicylic Acid 1.9 µm, 50 x 2.1 mm 12 4 5 Isocratic Mobile Phase: A: 0.1% formic acid in water 6 B: Methanol A:B, (65:35, v/v) Flow rate: 0.25 mL/min Temperature: 22 °C Detection: UV@240 nm Injection Vol.: 2 µL Sample: 1. Acetaminophen (Paracetamol, Tylenol) 2. Caffeine (1,3,7-Trimethylxanthine) 3. Salicylamide (o-Hydroxybenzamide) 4. Acetylsalicylic Acid (ASA, Aspirin) 5. Benzoic Acid 6. Salicylic Acid 3 1.02 02 .0 3.04 04 .0 5.06 06 .0 Time (min) B-7 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN LIFE SCIENCE

Colonnes HPLC/UHPLC Colonnes HPLC/UHPLC ACME Cannabis Physical Properties: SBeopnadreadtioPnhaMseo:d-eC:18RHe3v7e–rXsed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 450 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 100 Å Carbon Load (%): 22 Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: ≤ 98% USP Listing: L1 ACME Cannabis is a C18 bonded phase (L1) with optimized pore structure and phase loading for the separation of cannabinoids and cannabinoid-like compounds. It can also be used in applications where more retention is required when compared to the ACME C18. Dimensions 1.9 μm ACME Cannabis - 100 Å 5 μm Length (mm) x ID ACMCB-1.9-03021 3 μm ACMCB-5-03021 2.1 x 30 ACMCB-1.9-03046 ACMCB-5-03046 4.6 x 30 ACMCB-1.9-05021 ACMCB-3-03021 ACMCB-5-05021 2.1 x 50 ACMCB-1.9-05030 ACMCB-3-03046 ACMCB-5-05030 3.0 x 50 ACMCB-1.9-05046 ACMCB-3-05021 ACMCB-5-05046 4.6 x 50 ACMCB-1.9-10021 ACMCB-3-05030 ACMCB-5-10021 2.1 x 100 ACMCB-1.9-10030 ACMCB-3-05046 ACMCB-5-10030 3.0 x 100 ACMCB-1.9-10046 ACMCB-3-10021 ACMCB-5-10046 4.6 x 100 ACMCB-1.9-15021 ACMCB-3-10030 ACMCB-5-15021 2.1 x 150 ACMCB-3-10046 ACMCB-5-15030 3.0 x 150 - ACMCB-3-15021 ACMCB-5-15046 4.6 x 150 - ACMCB-3-15030 ACMCB-5-25046 4.6 x 250 - ACMCB-3-15046 - ACME Cannabis - 100 Å Guard Columns Dimensions 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder ID x Length (mm) 2.1 x 5 ACMGC19UC521CB ACMGC3LC521CB ACMGC5LC521CB 3.0/4.6 x 5 ACMGC5LC546CB 2.1 x 10 ACMGC19UC546CB ACMGC3LC546CB ACMGC5LC1021CB 3.0/4.6 x 10 ACMGC5LC1046CB ACMGC19UC1021CB ACMGC3LC1021CB ACMGC19UC1046CB ACMGC3LC1046CB Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-8 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Cannabinoids ACME Cannabis Sample: 6. Cannabidiol (CBD) HPLC/UHPLC Columns 3 µm, 150 x 4.6 mm 1. Cannabidivarinic Acid (CBDVA) 7. Tetrahydrocannabivarin (THCV) A: 0.1% Formic Acid in Water, (v/v) 2. Cannabidivarin (CBDV) 8. Cannabinol (CBN) B: 0.095% Formic Acid in Acetonitrile, (v/v) 3. Cannabidiolic Acid (CBDA) 9. Δ9-tetrahydrocannabinol (THC Δ9) Gradient: 4. Cannabigerolic Acid (CBGA) 10. Δ8-tetrahydrocannabinol (THC Δ8) 5. Cannabigerol (CBG) 11. Cannabichromene (CBC) Profile Time AB 4. CBGA (min) 0 30 70 10 10 90 15 10 90 Flow rate: 1.5 mL/min 5. CBG 6. CBD Temperature: 25 °C Detection: UV@225 nm Injection Vol.: 2 μL 7. THCV 8. CBN 9. THC Δ9 1. CBDVA 8 2. CBDV 3. CBDA 10. THC Δ8 11. CBC 11 2 3 9 1 56 10 4 7 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-9 LIFE SCIENCE

Colonnes HPLC/UHPLC Colonnes HPLC/UHPLC ACME F5/C18 Physical Properties • F5/C18 can operate in 100% aqueous conditions and shows SBeopnadreadtioPnhaMseo:d-eC:18RHe3v7e–rXs-ePdF-PPhase improved retention of polar compounds Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm • Ultra-high purity (99.999%) extra-treated porous spherical Surface Area: 260 m2/g silica, for excellent peak shape and reproducibility Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 120 Å • Ultra-low bleed phase is suitable for use with UV and MS Carbon Load (%): 16 detection Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: 100% USP Listing: L1/L43 Bimodal phase combines C18 and PFP functional groups to increase selectivity in the separation of complex mixtures when compared to standard C18 phases alone. • Hydrophobic retention similar to C18 • PFP functionality provides alternate selectivity required for challenging applications Dimensions 1.9 μm ACME F5/C18 - 120 Å 5 μm Length (mm) x ID ACMF5C18-1.9-03021 3 μm ACMF5C18-5-03021 2.1 x 30 ACMF5C18-1.9-03046 ACMF5C18-5-03046 4.6 x 30 ACMF5C18-1.9-05021 ACMF5C18-3-03021 ACMF5C18-5-05021 2.1 x 50 ACMF5C18-1.9-05030 ACMF5C18-3-03046 ACMF5C18-5-05030 3.0 x 50 ACMF5C18-1.9-05046 ACMF5C18-3-05021 ACMF5C18-5-05046 4.6 x 50 ACMF5C18-1.9-10021 ACMF5C18-3-05030 ACMF5C18-5-10021 2.1 x 100 ACMF5C18-1.9-10030 ACMF5C18-3-05046 ACMF5C18-5-10030 3.0 x 100 ACMF5C18-1.9-10046 ACMF5C18-3-10021 ACMF5C18-5-10046 4.6 x 100 ACMF5C18-1.9-15021 ACMF5C18-3-10030 ACMF5C18-5-15021 2.1 x 150 ACMF5C18-3-10046 ACMF5C18-5-15030 3.0 x 150 - ACMF5C18-3-15021 ACMF5C18-5-15046 4.6 x 150 - ACMF5C18-3-15030 ACMF5C18-5-25046 4.6 x 250 - ACMF5C18-3-15046 - ACME F5/C18 - 120 Å Guard Columns Dimensions 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder ID x Length (mm) 2.1 x 5 ACMGC19UC521F5C18 ACMGC3LC521F5C18 ACMGC5LC521F5C18 3.0/4.6 x 5 ACMGC5LC546F5C18 2.1 x 10 ACMGC19UC546F5C18 ACMGC3LC546F5C18 ACMGC5LC1021F5C18 3.0/4.6 x 10 ACMGC5LC1046F5C18 ACMGC19UC1021F5C18 ACMGC3LC1021F5C18 ACMGC19UC1046F5C18 ACMGC3LC1046F5C18 Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-10 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Selectivity for Geometric Isomers Standard C18, 3µm HPLC/UHPLC Columns HPLC/UHPLC Columns 100 x 4.6 mm ACME F5/C18 F5/C18, 3µm Peaks 3 and 5 3 μm, 100 x 4.6 mm 100 x 4.6 mm unresolved Mobile Phase: Methanol:Water, (40:60, v/v) 3 45 Flow rate: 1.5 mL/min Temperature: 22 °C Detection: UV@225 nm Injection Vol.: 3 μL Sample: 1. Void peak 2. Impurity peak 3. 1,2,4-trimethoxybenzene 4. 1,2-dimethoxybenzene 5. 1, 2,3-trimethoxybenze Phase Selectivity C18 vs ACME F5/C18 ACME F5/C18, 3 µm, 100 x 4.6 mm Flow rate: 1.5 mL/min 7 ACME PLUS, 3 µm, 100 x 4.6 mm Temperature: 25 °C Mobile Phase: Detection: UV@245 nm ACME PLUS A: 25 mM Ammonium Formate in water Injection Vol.: 3 µL B: Acetonitrile Gradient: Standard C18, 3µm 100 x 4.6 mm 3 2 5 Profile 13 AB Time (min) 8 10 12 9 11 1 4 6 0 100 0 14 10 35 65 11 10 90 15 10 90 7 F5/C18, 3µm 100 x 4.6 mm Sample: 8. Verapamil ACME F5C18 1. Uracil 9. Propranolol 2. Adenine 10. Oxazepam 13 3. Atenolol 11. Fentanyl 4. Pindolol 12. Tolbutamide 3 5 5. Codeine 13. Diazepam 6. Norfentanyl 14. D9-Tetrahydrocannabinol 2 10 7. Acebutolol 1 8 9 12 6 4 14 11 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-11 LIFE SCIENCE

Colonnes HPLC/UHPLC ACME Amide/C18 Colonnes HPLC/UHPLC Physical Properties SBeopnadreadtioPnhaMseo:d–e(:CRHe2v)3eNrHse(dC-=POh)a-Cse15H37 Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 260 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 120 Å Carbon Load (%): 19 Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: 100% USP Listing: L1/L60 Bimodal phase combines C18 and an embedded Amide • Ultra-high purity (99.999%) extra-treated porous spherical functional group to increase selectivity in the separation of silica, for excellent peak shape complex mixtures when compared to standard C18 phases alone. and reproducibility • Hydrophobic retention similar to C18 • Ultra-low bleed phase is suitable for use with UV and MS • Amide functionality provides alternate selectivity required detection for challenging applications • Similar retention window with ACME C18 • Amide/C18 can operate in 100% aqueous conditions and shows improved retention of polar compounds. Dimensions 1.9 μm ACME Amide/C18 - 120 Å 5 μm Length (mm) x ID ACMAM-1.9-03021 3 μm ACMAM-5-03021 2.1 x 30 ACMAM-1.9-03046 ACMAM-5-03046 4.6 x 30 ACMAM-1.9-05021 ACMAM-3-03021 ACMAM-5-05021 2.1 x 50 ACMAM-1.9-05030 ACMAM-3-03046 ACMAM-5-05030 3.0 x 50 ACMAM-1.9-05046 ACMAM-3-05021 ACMAM-5-05046 4.6 x 50 ACMAM-1.9-10021 ACMAM-3-05030 ACMAM-5-10021 2.1 x 100 ACMAM-1.9-10030 ACMAM-3-05046 ACMAM-5-10030 3.0 x 100 ACMAM-1.9-10046 ACMAM-3-10021 ACMAM-5-10046 4.6 x 100 ACMAM-1.9-15021 ACMAM-3-10030 ACMAM-5-15021 2.1 x 150 ACMAM-3-10046 ACMAM-5-15030 3.0 x 150 - ACMAM-3-15021 ACMAM-5-15046 4.6 x 150 - ACMAM-3-15030 ACMAM-5-25046 4.6 x 250 - ACMAM-3-15046 - ACME Amide/C18 - 120 Å Guard Columns Dimensions 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder ID x Length (mm) 2.1 x 5 ACMGC19UC521AM ACMGC3LC521AM ACMGC5LC521AM 3.0/4.6 x 5 ACMGC5LC546AM 2.1 x 10 ACMGC19UC546AM ACMGC3LC546AM ACMGC5LC1021AM 3.0/4.6 x 10 ACMGC5LC1046AM ACMGC19UC1021AM ACMGC3LC1021AM ACMGC19UC1046AM ACMGC3LC1046AM Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-12 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Column Type Phase Selectivity C18 vs Amide/C18 ACME Amide/C18 C18 Phase 2 3 Phenols and Carboxylic acids HPLC/UHPLC Columns 3 μm, 100 x 2.1 mm 1 Isocratic Mobile Phase: 8 ACME C18 In the file you sent you have th Acetonitrile:Water:Formic Acid, 46 listed as a polar embedded. Pl (30:70:0.1, v/v/v) it as ACME Amide Flow rate: 1.0 mL/min 5 Temperature: 325 °C 7 9 Detection: UV@254 nm Injection Vol.: 3 μL Sample: 3 ACME Amide/C18 1. Void marker 2 2. Resorcinol 3. 4-Methoxyphenol ACME Amide 4 6 8 4. Phenol 5 7 9 5. 4-Methoxybenzoic Acid 1 6. 4-Methylphenol 7. 4-Methylbenzoic Acid 8. 4-Nitrophenol 9. 3,4-Dimethylphenol Tricyclic Antidepressants Hydrophobic Basic Pharmaceuticals 2 1 ACME Amide/C18 3 μm, 100 x 4.6 mm Sample: 3 20mM Ammonium Phosphate (Monobasic) 1. Nordoxepin 4 Flow rate: 1.0 mL/min 2. Desipramine Temperature: 30 °C 3. Doxepin Detection: UV@254 nm 4. Imipramine Injection Vol.: 5 μL Amide shows incredibly good peak shape Amide ACME Amide/C18 on hydrophobic basic pharmaceuticals. mine / Fluoxetine 1 ACME Amide/C18 3 Sample: 1. Diphenhydramine 2. Nor-Fluoxetine 3. Fluoxetine 2 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-13 LIFE SCIENCE

Colonnes HPLC/UHPLC Colonnes HPLC/UHPLC ACME PLUS Physical Properties SBeopnadreadtioPnhaMseo:dCe1:8HRe37versed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 200 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 200 Å Carbon Load (%): 12 Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: 100% USP Listing: L1 The ACME PLUS is a truly unique HPLC phase. It is made on a 200 Å silica using precision bonding and end capping. The large pore structure allows for: • Facile separation of proteins and peptides • 100% aqueous mobile phase compatibility with good retention for analysis of small molecules • Faster separations on many standard analyses Dimensions 1.9 μm ACME PLUS - 200 Å 5 μm ID x Length (mm) ACMP-1.9-03021 3 μm ACMP-5-03021 2.1 x 30 ACMP-1.9-03046 ACMP-5-03046 4.6 x 30 ACMP-1.9-05021 ACMP-3-03021 ACMP-5-05021 2.1 x 50 ACMP-1.9-05030 ACMP-3-03046 ACMP-5-05030 3.0 x 50 ACMP-1.9-05046 ACMP-3-05021 ACMP-5-05046 4.6 x 50 ACMP-1.9-10021 ACMP-3-05030 ACMP-5-10021 2.1 x 100 ACMP-1.9-10030 ACMP-3-05046 ACMP-5-10030 3.0 x 100 ACMP-1.9-10046 ACMP-3-10021 ACMP-5-10046 4.6 x 100 ACMP-1.9-15021 ACMP-3-10030 ACMP-5-15021 2.1 x 150 ACMP-3-10046 ACMP-5-15030 3.0 x 150 - ACMP-3-15021 ACMP-5-15046 4.6 x 150 - ACMP-3-15030 ACMP-5-25046 4.6 x 250 - ACMP-3-15046 Dimensions - ID x Length (mm) ACME PLUS - 200 Å Guard Columns 2.1 x 5 3.0/4.6 x 5 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges 2.1 x 10 *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder 3.0/4.6 x 10 ACMGC19UC521P ACMGC3LC521P ACMGC5LC521P ACMGC5LC546P ACMGC19UC546P ACMGC3LC546P ACMGC5LC1021P ACMGC5LC1046P ACMGC19UC1021P ACMGC3LC1021P ACMGC19UC1046P ACMGC3LC1046P Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-14 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Water Soluble Vitamins HPLC/UHPLC Columns ACME PLUS Sample: 6HPLC/UHPLC Columns 3 µm, 100 x 4.6 mm 1. Thiamine (Vitamin B1) 5 A: 50 mM KH2PO4 buffer (pH 3.0) 2. Pyridoxine (Vitamin B6) 4 B: Methanol 3. Niacinamide (Nicotinamide, Vitamin B3) 4. Folic Acid (Vitamin B9) Gradient: 5. Cyanocobalamin (Vitamin B12) 6. Riboflavin (Vitamin B2) Time Profile (min) AB 2 3 0 98 2 10 50 50 Flow rate: 1.0 mL/min Temperature: 30 °C Detection: UV@270 nm Injection Vol.: 10 µL 0 2.0 4.0 6.0 8.0 10.0 12.0 Time (min) Phenols 3 ACME PLUS 2 3 µm, 100 x 4.6 mm Isocratic Mobile Phase: 45 6 A: Water B: Acetonitrile 17 A:B, (75:25, v/v) Flow rate: 1.5 mL/min 89 Temperature: 25 °C Detection: UV@268 nm Injection Vol.: 1 µL Sample: 1. Uracil 2. Resorcinol 3. 4-Hydroxybenzaldehyde 4. Phenol 5. Methylparaben 6. p-Nitrophenol 7. p-Cresol 8. 3,4-Dimethylphenol 9. 2,6-Dimethylphenol 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-15 LIFE SCIENCE

Colonnes HPLC/UHPLCColonnes HPLC/UHPLC ACME C8 Physical Properties BSeopnadreadtioPnhaMseo:d-eC:8HRe17versed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 260 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 120 Å Carbon Load (%): 10 Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: ≤ 98% USP Listing: L7 Dimensions 1.9 μm ACME C8 - 120 Å 5 μm Length (mm) x ID ACMC8-1.9-03021 3 μm ACMC8-5-03021 2.1 x 30 ACMC8-1.9-03046 ACMC8-5-03046 4.6 x 30 ACMC8-1.9-05021 ACMC8-3-03021 ACMC8-5-05021 2.1 x 50 ACMC8-1.9-05030 ACMC8-3-03046 ACMC8-5-05030 3.0 x 50 ACMC8-1.9-05046 ACMC8-3-05021 ACMC8-5-05046 4.6 x 50 ACMC8-1.9-10021 ACMC8-3-05030 ACMC8-5-10021 2.1 x 100 ACMC8-1.9-10030 ACMC8-3-05046 ACMC8-5-10030 3.0 x 100 ACMC8-1.9-10046 ACMC8-3-10021 ACMC8-5-10046 4.6 x 100 ACMC8-1.9-15021 ACMC8-3-10030 ACMC8-5-15021 2.1 x 150 ACMC8-3-10046 ACMC8-5-15030 3.0 x 150 - ACMC8-3-15021 ACMC8-5-15046 4.6 x 150 - ACMC8-3-15030 ACMC8-5-25046 4.6 x 250 - ACMC8-3-15046 Dimensions - ID x Length (mm) ACME C8 - 120 Å - 120 Å Guard Columns 2.1 x 5 3.0/4.6 x 5 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges 2.1 x 10 *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder 3.0/4.6 x 10 ACMGC19UC521C8 ACMGC3LC521C8 ACMGC5LC521C8 ACMGC5LC546C8 ACMGC19UC546C8 ACMGC3LC546C8 ACMGC5LC1021C8 ACMGC5LC1046C8 ACMGC19UC1021C8 ACMGC3LC1021C8 ACMGC19UC1046C8 ACMGC3LC1046C8 Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-16 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Caffeine Metabolites 3 5 HPLC/UHPLC Columns 2 ACME C8 3 µm, 100 x 4.6 mm 1 Isocratic Mobile Phase: 4 A: 0.1% formic acid in water B: Methanol ACME C8 A:B, (80:20, v/v) Flow rate: 1.0 mL/min Temperature: 30 °C Detection: UV@270 nm Injection Vol.: 2 µL Sample: 3 5 1. Theobromine (3,7-Dimethylxanthine) 2 2. Paraxanthine (1,7-dimethylxanthine) 3. Theophylline (1,3-Dimethylxanthine) 1 4. 1,3-Dimethyl-7-(2-hydroxyethyl)xanthine 4 5. Caffeine (1,3,7-Trimethylxanthine) ACME C18 1 ACME C8 23 65% Methanol Tricyclic Antidepressants 4 6 ACME C8 5 3 µm, 100 x 4.6 mm Isocratic Mobile Phase: 1 ACME C18 A: 25 mM ammonium phosphate buffer 2 70% Methanol (pH 7.0) 3 B: Methanol 45 A:B, (35:65, v/v) Flow rate: 1.5 mL/min Temperature: 35 °C Detection: UV@254 nm Injection Vol.: 2 µL Sample: 1. Nordoxepin 2. Desipramine 3. Nortriptyline 4. Doxepin 5. Imipramine 6. Amitriptyline 6 1.0 2.0 3.0 4.0 5.0 6.0 7.0 Time (min) B-17 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN LIFE SCIENCE

Colonnes HPLC/UHPLCColonnes HPLC/UHPLC ACME CN (Cyano) Physical Properties Bonded Phase: -R-CN Separation Mode: Reversed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 260 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 120 Å Carbon Load (%): 7 Endcapping: Yes proprietary pH Range: 2 to 7.5 Temperature Limit: 50 °C Aqueous Compatibility: 100% USP Listing: L10 Dimensions 1.9 μm ACME CN - 120 Å 5 μm Length (mm) x ID ACMCN-1.9-03021 3 μm ACMCN-5-03021 2.1 x 30 ACMCN-1.9-03046 ACMCN-5-03046 4.6 x 30 ACMCN-1.9-05021 ACMCN-3-03021 ACMCN-5-05021 2.1 x 50 ACMCN-1.9-05030 ACMCN-3-03046 ACMCN-5-05030 3.0 x 50 ACMCN-1.9-05046 ACMCN-3-05021 ACMCN-5-05046 4.6 x 50 ACMCN-1.9-10021 ACMCN-3-05030 ACMCN-5-10021 2.1 x 100 ACMCN-1.9-10030 ACMCN-3-05046 ACMCN-5-10030 3.0 x 100 ACMCN-1.9-10046 ACMCN-3-10021 ACMCN-5-10046 4.6 x 100 ACMCN-1.9-15021 ACMCN-3-10030 ACMCN-5-15021 2.1 x 150 ACMCN-3-10046 ACMCN-5-15030 3.0 x 150 - ACMCN-3-15021 ACMCN-5-15046 4.6 x 150 - ACMCN-3-15030 ACMCN-5-25046 4.6 x 250 - ACMCN-3-15046 Dimensions - ID x Length (mm) ACME CN - 120 Å - 120 Å Guard Columns 2.1 x 5 3.0/4.6 x 5 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges 2.1 x 10 *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder 3.0/4.6 x 10 ACMGC19UC521CN ACMGC3LC521CN ACMGC5LC521CN ACMGC5LC546CN ACMGC19UC546CN ACMGC3LC546CN ACMGC5LC1021CN ACMGC5LC1046CN ACMGC19UC1021CN ACMGC3LC1021CN ACMGC19UC1046CN ACMGC3LC1046CN Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-18 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Tricyclic Antidepressants under HILIC conditions HPLC/UHPLC Columns HPLC/UHPLC Columns ACME CN 1 3 µm, 100 x 4.6 mm Isocratic Mobile Phase: 3 A: 100 mM ammonium acetate in water 2 B: Acetonitrile A:B, (10:90, v/v) 4 Flow rate: 1.5 mL/min Temperature: 30 °C Detection: UV@245 nm Injection Vol.: 5 µL Sample: 1. Nordoxepin 2. Desipramine 3. Doxepin 4. Imipramine Fluoxetine and Metabolite ACME CN Sample: 1 3 µm, 100 x 4.6 mm 1. Norfluoxetine Isocratic Mobile Phase 2. Fluoxetine Flow rate: 1.5 mL/min Temperature: 30 °C 1 Detection: UV@230 nm Injection Vol.: 2 µL Reversed Phase Mode: HILIC Mode: Acetonitrile:25 mM ammonium Acetonitrile:100 mM ammonium phosphate in water, (45:55, v/v) acetate in water, (90:10, v/v) 2 2 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-19 LIFE SCIENCE

Colonnes HPLC/UHPLCColonnes HPLC/UHPLC ACME Ph (Phenyl) Physical Properties SBeopnadreadtioPnhaMseo:d-eR:-CR6eHv5ersed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 260 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 120 Å Carbon Load (%): 10 Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: 100% USP Listing: L11 Dimensions 1.9 μm ACME Ph - 120 Å 5 μm Length (mm) x ID ACMPH-1.9-03021 3 μm ACMPH-5-03021 2.1 x 30 ACMPH-1.9-03046 ACMPH-5-03046 4.6 x 30 ACMPH-1.9-05021 ACMPH-3-03021 ACMPH-5-05021 2.1 x 50 ACMPH-1.9-05030 ACMPH-3-03046 ACMPH-5-05030 3.0 x 50 ACMPH-1.9-05046 ACMPH-3-05021 ACMPH-5-05046 4.6 x 50 ACMPH-1.9-10021 ACMPH-3-05030 ACMPH-5-10021 2.1 x 100 ACMPH-1.9-10030 ACMPH-3-05046 ACMPH-5-10030 3.0 x 100 ACMPH-1.9-10046 ACMPH-3-10021 ACMPH-5-10046 4.6 x 100 ACMPH-1.9-15021 ACMPH-3-10030 ACMPH-5-15021 2.1 x 150 ACMPH-3-10046 ACMPH-5-15030 3.0 x 150 - ACMPH-3-15021 ACMPH-5-15046 4.6 x 150 - ACMPH-3-15030 ACMPH-5-25046 4.6 x 250 - ACMPH-3-15046 Dimensions - ID x Length (mm) ACME Ph - 120 Å - 120 Å Guard Columns 2.1 x 5 3.0/4.6 x 5 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges 2.1 x 10 *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder 3.0/4.6 x 10 ACMGC19UC521PH ACMGC3LC521PH ACMGC5LC521PH ACMGC5LC546PH ACMGC19UC546PH ACMGC3LC546PH ACMGC5LC1021PH ACMGC5LC1046PH ACMGC19UC1021PH ACMGC3LC1021PH ACMGC19UC1046PH ACMGC3LC1046PH Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-20 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Phenols ACME Ph 3 9 HPLC/UHPLC Columns ACME C18 2 45 ACME Ph 3 µm, 100 x 4.6 mm 6 Isocratic Mobile Phase: 7 A: 0.1% formic acid in water, (v/v) 1 B: 0.1% formic acid in methanol, (v/v) A:B, (75:25, v/v) 8 Flow rate: 1.0 mL/min Temperature: 25 °C 3 6 Detection: UV@270 nm Injection Vol.: 3 µL 2 Sample: 45 1. Uracil 2. Resorcinol 17 3. 4-Hydroxybenzaldehyde 4. Phenol 8 9 5. Methylparaben 6. p-Nitrophenol 7. p-Cresol 8. 3,4-Dimethylphenol 9. 2,6-Dimethylphenol Selectivity Analytes 4 3 ACME Ph 2 3 µm, 100 x 4.6 mm 1 Isocratic Mobile Phase: A: Water ACME Ph 3 B: Acetonitrile ACME C18 A:B, (50:50, v/v) 2 Flow rate: 1.5 mL/min 1 Temperature: 25 °C Detection: UV@220 nm 4 Injection Vol.: 5 µL Sample: 1. Ethylparaben 2. Acetophenone 3. Trinitrobenzene 4. Anisole 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-21 LIFE SCIENCE

Colonnes HPLC/UHPLC ACME PAH Colonnes HPLC/UHPLC Physical Properties SBeopnadreadtioPnhaMseo:d-e(C: 1R8Hev37e)rxsed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 200 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 200 Å Carbon Load (%): 17 Endcapping: No pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: ≤ 98% USP Listing: L1 ACME PAH - 200 Å Dimensions 3 μm 5 μm Length (mm) x ID ACMPAH-3-03021 ACMPAH-5-03021 2.1 x 30 ACMPAH-3-03046 ACMPAH-5-03046 4.6 x 30 ACMPAH-3-05021 ACMPAH-5-05021 2.1 x 50 ACMPAH-3-05030 ACMPAH-5-05030 3.0 x 50 ACMPAH-3-05046 ACMPAH-5-05046 4.6 x 50 ACMPAH-3-10021 ACMPAH-5-10021 2.1 x 100 ACMPAH-3-10030 ACMPAH-5-10030 3.0 x 100 ACMPAH-3-10046 ACMPAH-5-10046 4.6 x 100 ACMPAH-3-15021 ACMPAH-5-15021 2.1 x 150 ACMPAH-3-15030 ACMPAH-5-15030 3.0 x 150 ACMPAH-3-15046 ACMPAH-5-15046 4.6 x 150 ACMPAH-5-25046 4.6 x 250 - Polycyclic Aromatic Hydrocarbons Guard columns available upon request ACME PAH 24 PAH Sample: 9. Benzo(c)phenanthrene 17. Benzo(a)pyrene 3 µm, 150 x 4.6 mm A: Water 1. Naphthalene 10. Benz(a)anthracene 18. Dibenz(a,h)anthracene Mobile Phase: B: ACN 2. Acenaphthylene 1A1C.MChE rPyAsHen3.e0 19. Dibenzo(a,l)pyrene A: Water 30C 3. Acenaphthene 1125.07X, 12.21-mDmimethylbenz(a) 20. Benzo(g,h,i)perylene B: Acetonitrile 225nm 4. Fluorene 21. 3-Methylcholanthrene 0.5uL 5. Phenanthrene anthracene 22. Indeno(1,2,3-cd)pyrene 6. Anthracene 13. Benzo(j)fluoranthene 23. Dibenzo(a,h)pyrene Flow rate: See gradient table 7. Fluoranthene 3 14. Benzo(e)pyrene 24. Dibenzo(a,i)pyrene Temperature: 30 °C 8. Pyrene 15. Benzo(b)fluoranthene Detection: UV@263 nm 16. Benzo(k)fluoranthene Injection Vol.: 2 µL Gradient: Time ACN Flow 2 13 14 % 1 10 15 (min) (mL/min) 11 0 50 0.3 79 16 10 100 0.3 21 16 16.5 100 0.3 5 8 12 17181920 22 100 0.6 46 Solvent 23 24 0 10 20 Time (min) B-22 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE