CLS Sample Prep

Customer Service About Canadian Life Science Au suject de Canadian Life Science Founded in 1997, Canadian Life Science (CLS) has steadily grown Fondée en 1997, Canadian Life Science (CLS) n’a cessé de croître to Canada’s leading supplier of LC/GC columns, chromatography pour devenir un chef de file canadien dans la vente de colonnes and dissolution accessories as well as 2d long-term sample HPLC / GC, d’accessoires de chromatographie et dissolution, storage tubes and racks for bio-banking and compound ainsi que dans la vente de tubes et systèmes d’entreposage long management. terme pour échantillons. For over 22 years, CLS has provided customers with unparalleled Depuis plus de 22 ans, CLS offre à ses clients un service et service, expertise and a wide selection of quality and cost- une expertise inégalés, en plus d’un large éventail de produits effective products that offer maximum flexibility to solve your économiques de qualité offrant une flexibilité maximale afin de analytical challenges. CLS is your foundation for what matters. résoudre vos problèmes analytique. CLS est votre pilier pour ce qui compte. Everyone is accessible to you Nous sommes très accessible We believe that our customers like our personal touch. Nous avons une approche personnelle et nous répondons à tous We take your calls personally, not a computer, not voicemail . les appels lorsque vous téléphonez au bureau chef. Nous n’avons We have dedicated inside customer service staff who care, and pas de boîte vocale. take care for specific areas. We have knowledgeable outside staff Notre personnel est dévoué au service à la clientèle et ce à based in most of the larger cities accross Canada. travers le Canada. Si vous désirez parler directement à notre We supply world class products Directrice de comptes au Québec, vous pouvez contacter Canadian Life Science distributes for top-quality manufacturers. Genevieve Lemieux au 514.428.8034. Our product offering has been chosen in a way that will give you Nous choisissons les meilleurs produits au monde the maximum flexibility solving your analytical challenges. En tant que distributeur nous avons l’avantage de choisir les Technical Support meilleurs fournisseurs et notre gamme complète de produit vous Our technical staff is happy to respond to customer enquiries. donne l’embarras du choix. We can also supply extensive application information from one Support Technique of the world’s largest data bases. Notre équipe technique se fait toujours un plaisir de vous aider à Plan the best delivery faire le meilleur choix pour vos applications. When you decide to purchase a product from Canadian Life Service et logistique Science our customer service department will find the fastest Lorsque vous placez une commande chez nous, nous nous and most economical way to send your order to you. For assurons de choisir le moyen le plus efficace et économique your convenience we have two warehouse locations, one in d’acheminer la marchandise. Edmonton, AB and one in Peterborough, ON. Nous avons deux entrepôts dont un en Alberta pour servir nos We’re glad to help clients de l’Ouest et un en Ontario pour servir nos clients de l’Est. We are never too busy to take your call. C’est un plaisir de vous servir Try us. You’ll notice the difference. Nous ne sommes jamais trop occupés pour vous répondre. Canadian Life Science, friendly people to do business with! Essayez-nous et vous verrez la différence. Il est agréable de faire affaire avec nous! I-22 CANADIAN Proud member of the Chrom4 buying group Fier membre du groupe dʼacheteur Chrom4 LIFE SCIENCE 1 888-226-2775 :: [email protected] :: www.lifescience.ca

SPE Introduction How to choose an SPE product SPE methods 1. Characterize the sample By syringe Factors such as the analyte’s polarity relative to the Process individual matrix, the presence of charged functional groups, samples via luer hub solubility, molecular weight, etc., determine how strong syringe. Connect the analyte is retained by the packed bed. syringe directly to SPE cartridges or to SPE 2. Select a retention strategy columns via adapters. There are two basic methods for sample treatment: By vacuum manifold a. Select the packing bed to retain the desired analyte. The contaminants are washed off and the desired Process multiple analyte is then eluted for analysis. samples on a vacuum manifold using b. Select the packing bed to retain the contaminants SPE columns. Extend and the desired analyte passes directly through. volume via reservoirs. 3. Select the proper packing type By filter flask Select the proper packing type for the cleanest extract Process large volume with the highest recovery. samples by placing SPE tube with an a. Reversed-phase packings are hydrophobic, silica-based online filtration system materials that retain moderately polar to non-polar compounds from a polar matrix while washing off polar For Environmental Applications interferences. Or you can retain non-polar contaminants while the polar compounds pass through unretained. Recommended SW-846 Cleanup Methods b. Normal-phase packings are hydrophilic, silica-based Analyte Group EPA Recommended materials that retain polar compounds from a non- Method No. SPE Sorbents polar matrix while washing off non-polar interferences. Or you can retain polar contaminants while non-polar Aniline and Derivatives 3620 C18 compounds pass through unretained. C18, Florisil® Chlorinated Pesticides 3620 C18, Phenyl c. Ion-exchange resins retain charged compounds or C18, Florisil® remove ionic interferences. Chlorinated Hydrocarbons 3620 DVB, IC-RP 4. Optimize conditions for best results Haloethers 3620 C18 C18, Florisil® Select proper bed size and suitable conditioning, wash Nitroaromatics and Cyclic 3620 Florisil®, Carbograph and elution solvents. Ketones C18, SCX, Carbograph a. Poor sample recovery often occurs when the packed Nitrosamines 3610, 3620 Alumina bed dimensions are not optimized. An excessive bed C18, Phenyl weight results in incomplete elution while an insufficient Organochlorine Pesticides 3620 C18, DVB bed weight results in incomplete retention. C18, Phenyl Organophosphorus Pesticides 3620 b. Consider the solvent strength relative to the packing material. The final conditioning solvent should be PCB’s 3620, 3630 weak so it doesn’t act as an eluting solvent. Buffers should be used to control ionization of potentially charged compounds. c. Wash solvents should remove weakly retained interferences without being strong enough to elute the analyte. d. Elution solvents should be strong enough to completely elute an analyte in a small volume (1–2mL). Petroleum Waste 3611 Phenols 3630 Phthalate Esters 3610, 3620 Polynuclear Aromatic 3630 Hydrocarbons 418 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

SPE Introduction SPE method development As Canadaʼs leading GC and LC column supplier, we have 1 CONDITION by aspirating a few milliliters of suitable gained an unrivalled reputation for excellent quality, com- petive pricing and fast delivery. solvent or solution through the column. InnoSep™ SPE cartridges are available in a wide variety of 2 ADD SAMPLE - extract and concentrate compounds of chemically bonded phases for rapid sample preparation by interest by aspirating a sample solution. adsorption and elution. See page 422 for silica based ANALYTE (InnoSep™) products. INTERFERANCE 3 WASH - remove co-extracted interferences by For polymer phase selection, see our IRIS products. aspirating 2mL or less of suitable solvent /solution. 4 ELUTE extracted compounds by aspirating This polymer based product is found from page 424 onwards. 1mL or less of suitable solvent /solution You will find a variety of chemistries (e.g. MCX, MAX, neutral and our new WAX and WCX) Discard If you are not familiar or are new to SPE, please contact us. Discard Discard Analyze General method development procedures Reversed-phase extraction procedure Step 1—Condition Step 2—Load Step 3—Wash Step 4—Elute Mechanism: Bind moderately polar to non-polar compounds from a polar sample matrix Methanol followed Process sample at a Water or water: Methanol or acetonitrile. May need to Normal-phase extraction procedure Mechanism: Bind polar compounds from by water flow rate of 1–5mL/min methanol (95:5) add strong acid or base to organic solvent a non-polar sample matrix Ion-exchange extraction procedure to break secondary interactions. Mechanism: Bind charged (negative/anionic or positive/cationic) compounds IPA followed Process sample at a Hexane or IPA, ethyl acetate, acetone, or hexane: by hexane flow rate of 1–5mL/min hexane:IPA IPA (50:50) (98:2) Methanol:water Apply slowly: less than Methanol:low High ionic strength (0.5M–1.0M) buffer or modify pH such that the analyte is (50:50) followed by or equal to 1mL/min ion ionic strength uncharged. May need to add organic to break hydrophobic interactions. low ionic strength exchange kinetics are (0.1M) buffer (0.1M) buffer slower than reversed- (10:90) or normal-phase Recommended usage guidelines* 50mg 100mg 200mg 500mg 500mg 1000mg 2000mg 5000mg 10,000mg 2.5mg 5mg 10mg 25mg 25mg 50mg 100mg 250mg 500mg Bed Size: Sorbent Retention Capacity: 0.30mL 0.60mL 1.20mL 3.00mL 3.00mL 6.00mL 12.00mL 30.00mL 60.00mL 0.45mL 0.90mL 1.80mL 4.50mL 4.50mL 9.00mL 18.00mL 45.00mL 90.00mL Condition Volume (4-bed volumes) 0.23mL 0.45mL 0.90mL 2.25mL 2.25mL 4.50mL 9.00mL 22.50mL 45.00mL Wash Volume (6-bed volumes) Minimum Elution Volume (3-bed volumes) *Estimates only. Must optimize for each application. TIP! Solvent Polarity Chart 1.To calculate sorbent bed volume, use 150µL for every 100mg Less Polar (Stronger Reversed-Phase Elution) of sorbent. Hexane Isooctane 2.Retention capacity describes the total amount that an SPE Cyclohexane Carbon tetrachloride sorbent will bind. This includes all compounds retained—analytes Toluene of interest as well as the contaminants. Chloroform Methylene chloride 3.Minimum elution volume recommended in bed size chart above Tetrahydrofuran Ethyl ether will offer best sensitivity, but more solvent may be required Acetone depending on application. Acetonitrile Pyridine Isopropanol Ethanol Methanol Water Acetic acid More Polar (Stronger Normal-Phase Elution) 419

SPE Introduction Packing Description of Base Sorbent Retention Mechanism Non-Polar C18/14 Octadecyl Carbon Loading: 14% Endcapped 40μm irregularly-shaped silica, The primary retention mechanism is strongly non-polar Carbon Loading: 18% Endcapped 60Å mean porosity with typical sample matrices of water C18/18 Octadecyl Carbon Loading: 22% Endcapped and aqueous biological fluids. 40μm irregularly-shaped silica, The primary retention mechanism is strongly non-polar C18/22 Octadecyl 60Å mean porosity with typical sample matrices of water and aqueous biological fluids. 40μm irregularly-shaped silica, The primary retention mechanism is strongly non-polar 60Å mean porosity with typical sample matrices of water and aqueous biological fluids. C18/O Octadecyl Carbon Loading: 13% Not Endcapped 40μm irregularly-shaped silica, The primary retention mechanism is moderately H 60Å mean porosity. non-polar with typical sample matrix of aqueous biological fluids. The lack of endcapping results in C8 Octyl Carbon Loading: 12% Endcapped 40μm irregularly-shaped silica, significant polar secondary interactions. Carbon Loading: 11% Endcapped 60Å mean porosity The primary retention mechanism is moderately PH Phenyl Carbon Loading: 9% Endcapped non-polar with typical sample matrices of water Carbon Loading: 9% Endcapped 40μm irregularly-shaped silica, and aqueous biological fluids. CH Cyclohexyl Carbon Loading: 6% Endcapped 60Å mean porosity. The primary retention mechanism is moderately Carbon Loading - 3% non-polar with typical sample matrices of water C4 Butyl Carbon Loading: 8% Endcapped 40μm irregularly-shaped silica, and aqueous biological fluids. 60Å mean porosity. The primary retention mechanism is moderately C2 Ethyl non-polar with typical sample matrices of water 40μm irregularly-shaped silica, and aqueous fluids. C1 Methyl 60Å mean porosity. The primary retention mechanism is moderately CNe Cyanopropyl non-polar with typical sample matrices of water 40μm irregularly-shaped silica, and aqueous biological fluids. 60Å mean porosity. The primary retention mechanism is weakly non-polar with typical sample matrices of plasma, 40μm irregularly-shaped silica, urine and aqueous fluids. 60Å mean porosity. The primary retention mechanism is very weakly non-polar with typical sample matrices of plasma, 40μm irregularly-shaped silica, urine and aqueous fluids. 60Å mean porosity. The primary retention mechanism is very weakly non-polar and is used with aqueous sample matrices. Polar CN Cyanopropyl Carbon Loading: 7% Not Endcapped 40μm irregularly-shaped silica, The primary retention mechanism is polar DIO Diol Carbon Loading: 7% with typical sample matrices of non-polar solvents. SI Silica Gel 60Å mean porosity. FLO Florisil® Florisil® is a registered trademark ALA Alumina of Floridin Co. 40μm irregularly-shaped silica, The primary retention mechanism is polar Acidic 60Å mean porosity. with typical sample matrices of non-polar ALN Alumina organic extracts, oil and lipids. Neutral ALB Alumina 40μm irregularly-shaped silica, The primary retention mechanism is strongly polar Basic 60Å mean porosity. with typical sample matrices of non-polar organic solvents, oils and lipids. Florisil®, magnesium sulfate, The primary retention mechanism is polar 150 - 250μm particle size. with environmental and organic extracts as typical sample matrices. Alumina, Al2O3, Activity I: The primary retention mechanisms are polar, acidic particle size 50 - 200μm, Lewis acid/base and ion exchange. pH 4.5 (Activity Levels II, III, IV, and V also available. Please inquire.) Alumina, Al2O3, The primary retention mechanisms are polar, neutral particle size 50 - 200μm, Lewis acid/base and ion exchange. pH 7.5 All alumina activity I. (Activity II, III, IV, V also available. Please inquire.) Alumina, Al2O3, The primary retention mechanisms are polar, basic particle size 50 - 200μm, Lewis acid/base and ion exchange. pH 10 All alumina activity I. (Activity II, III, IV, V also available. Please inquire.) Ionic Exchange COOH Carboxylic Acid Ion Exchange Capacity - 0.3 meq/gm 40μm irregularly-shaped silica, The primary retention mechanism is 60Å mean porosity. weak cation exchange with typical sample matrices of water and biological fluids. SCX Benzenesulfonic Ion Exchange Capacity - 0.8 meq/gm 40μm irregularly-shaped silica, The primary retention mechanism is Acid 60Å mean porosity. strong cation exchange with typical sample matrices of aqueous, biological fluids. NH2 Aminopropyl Carbon Loading - 40μm irregularly-shaped silica, The primary retention mechanism is 5% Ion Exchange Capacity 60Å mean porosity. weak anion exchange with typical sample PSA - 0.8 meq/gm matrices of water, biological fluids, and DEA 40μm irregularly-shaped silica, organic extracts. N+ Primary/Secondary Ion Exchange Capacity 60Å mean porosity. The primary retention mechanism is weak Amine - 1.4 meq/gm 40μm irregularly-shaped silica, anion exchange with aqueous samples. 60Å mean porosity. Diethylamino Ion Exchange Capacity The primary retention mechanism is - 0.9 meq/gm 40μm irregularly-shaped silica, weak anion exchange with aqueous samples. 60Å mean porosity. The primary retention mechanism is Quaternary Amino Ion Exchange Capacity strong anion exchange with typical sample - 0.8 meq/gm matrices of aqueous and biological fluids. 420 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

SPE Introduction Overview of SPE Cartridges and 96-Well Plates SPE columns • Open top tubes with male luer bottom, available with or without flange • Process multiple samples with vacuum manifold or automated SPE instruments • Process individual samples manually with use of adapter and syringe 1mL 3mL 6mL 15mL SPE cartridges 25mL • Female luer top and male luer bottom SPE 96-well plates • Process single cartridge by syringe • High throughput SPE devices or multiple cartridges by vacuum • 8\" x 12\" standard 96-well format • Stack for multi-step extractions • 2mL square wells Phos-Tio Kits 200 L / 3mg Collection Tubes & Waste Tubes 300mg Bed 600mg Bed 10 L / 1mg 900mg Bed Tip column Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 421

InnoSep™ SPE Cartridges and 96-Well Plates InnoSep™ SPE Cartridges Empty reservoirs available Easy to use • Sample preparation in four simple steps • Reduces use of hazardous solvents by 95% Economical, environmentally friendly • Reduces use of hazardous solvents by 95% • Reduces operator time, glassware and equipment by 83% Reliable Ordering Information • InnoSep™ offers the tightest quality control Part No. 100/box 50/box 50/box 30/box 30/box for particle size, surface activity, pH, flow rate Phase 100mg 200mg 500mg 500mg 1000mg and interferences 1mL 3mL 3mL 6mL 6mL • Hermetically sealed packets prevent contamination C18 Octadecyl C18/14% IS72001 IS72002 IS72003 IS72006 IS72007 and allow inspection of the cartridges before use C18 Octadecyl C18/18% IS12001 IS12002 IS12003 IS12006 IS12007 C18 Octadecyl C18/22% IS12101 IS12102 IS12103 IS12106 IS12107 C18 Octadecyl C18/OH% IS12201 IS12202 IS12203 IS12206 IS12207 C8 Octyl IS72011 IS72012 IS72013 IS72016 IS72017 1 CONDITION by aspirating a few milliliters of suitable PH Phenyl IS72081 IS72082 IS72083 IS72086 IS72087 solvent or solution through the column. CH Cyclohexyl IS72071 IS72072 IS72073 IS72076 IS72077 C4 Butyl IS72021 IS72022 IS72023 IS72026 IS72027 2 ADD SAMPLE - extract and concentrate compounds of C2 Ethyl IS72061 IS72062 IS72063 IS72066 IS72067 interest by aspirating a sample solution. C1 Methyl IS72051 IS72052 IS72053 IS72056 IS72057 Price $148.00 $ 98.00 $112.00 $ 74.00 $103.00 ANALYTE SI Silica Gel IS72101 IS72102 IS72103 IS72106 IS72107 INTERFERANCE FLO Florisil IS72111 IS72112 IS72113 IS72116 IS72117 ALN Alumina (Neutral) IS72121 IS72122 IS72123 IS72126 IS72127 3 WASH - remove co-extracted interferences by ALA Alumina (Acidic) IS72131 IS72132 IS72133 IS72136 IS72137 aspirating 2mL or less of suitable solvent /solution. 4 ELUTE extracted compounds by aspirating ALB Alumina (Basic) IS72141 IS72142 IS72143 IS72146 IS72137 1mL or less of suitable solvent /solution Price $126.00 $ 83.00 $ 98.00 $ 67.00 $ 86.00 CNe Cyanopropyl (ec)1 IS72221 IS72222 IS72223 IS72226 IS72227 CN Cyanopropyl IS72201 IS72202 IS72203 IS72206 IS72207 DIO Diol IS72151 IS72152 IS72153 IS72156 IS72157 COOH Carboxylic Acid IS72311 IS72312 IS72313 IS72316 IS72317 Discard SCX Benzenesulfonic Acid IS72321 IS72322 IS72323 IS72326 IS72327 NH2 Aminopropyl IS72211 IS72212 IS72213 IS72216 IS72217 PSA Pri/Sec Amino IS72241 IS72242 IS72243 IS72246 IS72247 Discard DEA Diethylamino IS72331 IS72332 IS72333 IS72336 IS72337 N+ Quaternary Amino IS72301 IS72302 IS72303 IS72306 IS72307 Price $178.00 $117.00 $143.00 $ 96.00 $133.00 1Endcapped Discard Analyze Use InnoSep™ cartridges for a wide range of applications- pharmaceutical, clinical, environmental, toxicological, biological, foods/cosmetics and agriculturall 422 Need Bulk Material? Silica, C18, Florisil & many others available. Please contact Canadian Life Science. Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

InnoSep™ SPE Cartridges and 96-Well Plates InnoSep™ 96-Well Plates Looking for Flash Chromatography? Please see pages 249 - 253. Solid Phase Extraction - 96-Well Format Economical Condition 1 • Prepare 96 samples at one time Condition by aspirating a few • Reduce use of hazardous solvents, operator time, microliters of suitable solvent or solution through the column. glassware and equipment Discard Reproducible 2 • Achieve high recoveries with 2-3% coefficients of Add Sample variation Versatile • Perform extractions with a wide variety of sorbents • Two plate sizes and a range of standard packing amounts are available Ordering Information Extract and concentrate compounds of interest by 25mg 50mg 25mg 50mg 100mg 200mg aspirating a sample solution. 1mL 3mL 2mL 2mL 2mL 2mL Phase C18 Octadecyl C18/14% IS24050 IS24051 IS24052 IS24053 IS24054 IS24055 C18 Octadecyl C18/18% IS24060 IS24061 IS24062 IS24063 IS24064 IS24065 Discard 3 C18 Octadecyl C18/22% IS24070 IS24071 IS24072 IS24073 IS24074 IS24075 Wash C18 Octadecyl C18/OH% IS24080 IS24081 IS24082 IS24083 IS24084 IS24085 C8 Octyl IS24100 IS24101 IS24102 IS24103 IS24104 IS24105 PH Phenyl IS24110 IS24111 IS24112 IS24113 IS24114 IS24115 Remove co-extracted interfer- ences by aspirating a few CH Cyclohexyl IS24120 IS24121 IS24122 IS24123 IS24124 IS24125 microliters of suitable solvent or solution. C4 Butyl IS24130 IS24131 IS24132 IS24133 IS24134 IS24135 C2 Ethyl IS24140 IS24141 IS24142 IS24143 IS24144 IS24145 C1 Methyl IS24150 IS24151 IS24152 IS24153 IS24154 IS24155 CNe Cyanopropyl (ec)1 IS24160 IS24161 IS24162 IS24163 IS24164 IS24165 Discard CN Cyanopropyl IS24170 IS24171 IS24172 IS24173 IS24174 IS24175 4 SI Silica Gel IS24180 IS24181 IS24182 IS24183 IS24184 IS24185 FLO Florisil IS24190 IS24191 IS24192 IS24193 IS24194 IS24195 ALN Alumina (Neutral) IS24200 IS24201 IS24202 IS24203 IS24204 IS24205 ALA Alumina (Acidic) IS24210 IS24211 IS24212 IS24213 IS24214 IS24215 Elute ALB Alumina (Basic) IS24220 IS24221 IS24222 IS24223 IS24224 IS24225 Elute extracted compounds by aspirating a few micro- DIO Diol IS24230 IS24231 IS24232 IS24233 IS24234 IS24235 liters of suitable solvent or solution. COOH Carboxylic Acid IS24240 IS24241 IS24242 IS24243 IS24244 IS24245 SCX Benzenesulfonic Acid IS24250 IS24251 IS24252 IS24253 IS24254 IS24255 NH2 Aminopropyl IS24260 IS24261 IS24262 IS24263 IS24264 IS24265 PSA Pri/Sec Amino IS24270 IS24271 IS24272 IS24273 IS24274 IS24275 DEA Diethylamino IS24280 IS24281 IS24282 IS24283 IS24284 IS24285 Analyze N+ Quaternary Amino IS24290 IS24291 IS24292 IS24293 IS24294 IS24295 Price $229.00 $253.00 $241.00 $266.00 $278.00 $290.00 1 Endcapped Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 423

IRIS SPE Method Development IRIS SPE Polymer Phase Selection IR IS IR5M42C4X6 60mg/3.0mL IRIS solid phase extraction cartridges allow for separations by reverse phase, normal phase, anion exchange, cation exchange and mixed modes. The diagram below is intended to simplify the process of selecting the right SPE product for your application. Sample Aqueous Organic Matrix Analyte Aqueous Organic Organic Solubility Analyte Ionic Neutral Neutral Neutral Ionic Charge Separation Anion Cation Reverse Reverse Normal Anion Cation Mode Exchange Exchange Phase Phase Phase Exchange Exchange Recommended IRIS MAX IRIS MCX IRIS N IRIS PLUS IRIS N IRIS MAX IRIS MCX Cartridge IRIS N IRIS WAX IRIS WCX IRIS WAX IRIS WCX IRIS IRIS General SPE Methods IRIS PLUS, IRIS N, IRIS IRIS N, IRIS IRIS MAX, IRIS MCX, IRIS WAX, IRIS WCX, 424 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

IRIS SPE Method Development *Polymeric gels provide larger surface area that requires larger solvent volumes per gram of sorbent. Elution volumes depend on the chemical nature of the analyte, its relative concentration in the matrix, the chemistry of the solution solvent and the sorbent bed mass. This table outlines a generic SPE method. Choosing the Right Cartridges Cartridge Size —Sample Capacity Sizes: 1 mL, 3 mL, 6 mL, 60 mL (Specialty sizes available upon request) The SPE Cartidge determines the volume of the sample solution, which can be loaded onto the sorbent bed in a single step. Pore Size—the mean diameter of the pores on the IRIS 100% Divinyl Benzene Bead 104Å sorbent particle surface IR5424M6CX 60mg/3.0mL The analyte molecule in an SPE method should fit easily through the pores of the sorbent (i.e. for large molecules, a sorbent particle with extra large pores should be selected). Resin Bed Mass—Analyte Capacity Sizes: 10 mg, 30 mg, 60 mg, 200 mg, 1 g (Specialty bed weights available upon request) The resin bed mass determines the maximum amount of analyte retained by the sorbent. Tips: Choosing the proper bed mass reduces leaching of sample components Tips for Choosing Proper Resin Mass Resin Bed Volume—Interstitial and Pore Volume Polymeric Sorbents—polymeric resins provide larger The resin bed volume controls the minimum solvent surface area than silica-based resins, offering higher volume in an SPE method. To successfully condition, capacity on the order of 10-15% of the resin mass. This wash and elute from the cartridge typically requires allows for the use of smaller bed volumes and improved solvent volumes 4 to 8 times that of the sorbent bed. detection limts. Using less than 4 to 8 sorbent bed volumes contributes to incomplete solvation and low, inconsistent recoveries. Ion Exchange Sorbents—the number of charged sites on the resin available to interact with the analyte Tips: Polymeric SPE products usually require 200-250 uL per 100 determines capacity. mg of sorbent. Tip: Choosing a slightly larger resin mass is advisable, since most SPE samples are loaded with contaminants and interferences Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 425

IRIS SPE Method Development Normal-Phase R R N HO O Phenol IRIS N, IRIS Normal-Phase Concepts Methodology 426 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

IRIS SPE Method Development Ion Exchange Sodium Salicylate R3 O O- N+ - R2 R1 Ion Exchange Concepts OH SO3- +H2N Acetominophen H3C O WAX,WCX Methodology Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 427

IRIS SPE Method Development - - 428 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

IRIS SPE Method Development Ordering Information Ordering Information Cartridges 96-Well Plates Description Qty Part No. Price Description Qty Part No. Price 30mg/1mL 100 IR74261 $156.00 10mg/1mL 1 IR10011 $300.00 50mg/1mL 100 IR74262 $227.00 25mg/1mL 1 IR24340 $309.00 100mg/1mL 100 IR74263 $389.00 30mg/1mL 1 IR10031 $300.00 60mg/3mL 50 IR74246 $120.00 50mg/1mL 1 IR24341 $352.00 100mg/3mL 50 IR74264 $195.00 60mg/1mL 1 IR10061 $342.00 200mg/3mL 50 IR74265 $346.00 25mg/2mL 1 IR24342 $321.00 60mg/6mL 30 IR74247 $346.00 30mg/2mL 1 IR10032 $312.00 200mg/6mL 30 IR74245 $220.00 60mg/2mL 1 IR10062 $35400 500mg/6mL 30 IR74266 $544.00 50mg/3mL 1 IR24343 $364.00 100mg/3mL 1 IR24344 $463.00 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 429

IRIS SPE Ordering Information Ordering Information IRIS N SPE Cartridges IRIS MCX SPE Cartridges Part No. Description Qty Price Part No. Description Qty Price (Box) (Box) IR44247 IRIS N 60mg/6.0mL 25-35µ \\30 $158 IR54247 IRIS MCX 60mg/6.0mL 25-35µ \\30 $213 IR44262 IRIS N 50mg/1.0mL 25-35µ \\100 $228 IR54262 IRIS MCX 50mg/1.0mL 25-35µ \\100 $268 IR44263 IRIS N 100mg/1.0mL 25-35µ \\100 $281 IR54263 IRIS MCX 100mg/1.0mL 25-35µ \\100 $281 IR44264 IRIS N 100mg/3.0mL 25-35µ \\100 $281 IR54264 IRIS MCX 100mg/3.0mL 25-35µ \\100 $281 IR44265 IRIS N 200mg/3.0mL 25-35µm \\100 $292 IR54265 IRIS MCX 200mg/3.0mL 25-35µ \\100 $292 IR44266 IRIS N 500mg/6.0mL 25-35µm \\30 $226 IR54266 IRIS MCX 500mg/6.0mL 25-35µ \\30 $226 IR44444 IRIS N 60mg/1.0mL 25-35µ \\100 $270 IR54444 IRIS MCX 60mg/1.0mL 25-35µ \\100 $270 IR44445 IRIS N 200mg/6.0mL 25-35µ \\30 $204 IR54445 IRIS MCX 200mg/6.0mL 25-35µ \\30 $204 IR44446 IRIS N 60mg/3.0mL 25-35µ \\100 $275 IR54446 IRIS MCX 60mg/3.0mL 25-35µ \\100 $275 IR44453 IRIS N 500mg/3.0mL 25-35µ \\50 $325 IR54453 IRIS MCX 500mg/3.0mL 25-35µ \\50 $325 IR44460 IRIS N 10mg/1.0mL 25-35µ \\100 $193 IR54460 IRIS MCX 10mg/1.0mL 25-35µ \\100 $193 IR44461 IRIS N 30mg/1.0mL 25-35µ \\100 $204 IR54461 IRIS MCX 30mg/1.0mL 25-35µ \\100 $204 IR44462 IRIS N 30mg/3.0mL 25-35µ \\100 $275 IR54462 IRIS MCX 30mg/3.0mL 25-35µ \\100 $275 IR44467 IRIS N 1000mg/60.0mL 25-35µ \\10 $138 IR54467 IRIS MCX 1000mg/60.0mL 25-35µ \\10 $138 IR44468 IRIS N 10mg/1.0cc 55-65µ, 80A \\100 $193 IR54468 IRIS MCX 10mg/1.0cc 55-65µ, 80A \\100 $193 IR44469 IRIS N 30mg/1.0cc 55-65µ, 80A \\100 $204 IR54469 IRIS MCX 30mg/1.0cc 55-65µ, 80A \\100 $204 IR44470 IRIS N 60mg/1.0cc 55-65µ, 80A \\100 $270 IR54470 IRIS MCX 60mg/1.0cc 55-65µ, 80A \\100 $270 IR44471 IRIS N 100mg/1.0cc 55-65µ, 80A \\100 $281 IR54471 IRIS MCX 100mg/1.0cc 55-65µ, 80A \\100 $281 IR44472 IRIS N 60mg/3.0cc 55-65µ, 80A \\100 $275 IR54472 IRIS MCX 60mg/3.0cc 55-65µ, 80A \\100 $275 IR44473 IRIS N 200mg/3.0cc 55-65µ, 80A \\100 $292 IR54473 IRIS MCX 200mg/3.0cc 55-65µ, 80A \\100 $292 IR44474 IRIS N 500mg/3.0cc 55-65µ, 80A \\50 $325 IR54474 IRIS MCX 500mg/3.0cc 55-65µ, 80A \\50 $325 IR44475 IRIS N 200mg/6.0cc 55-65µ, 80A \\30 $204 IR54475 IRIS MCX 200mg/6.0cc 55-65µ, 80A \\30 $204 IR44476 IRIS N 500mg/6.0cc 55-65µ, 80A \\30 $226 IR54476 IRIS MCX 500mg/6.0cc 55-65µ, 80A \\30 $226 IR44477 IRIS N 1000mg/6.0cc 55-65µ, 80A \\10 $138 IR54477 IRIS MCX 1000mg/60.0cc 55-65µ, 80A \\10 $138 IR44478 IRIS N 10000mg/6.0cc 55-65µ, 80A $402 IR54478 IRIS MCX 10000mg/60.0cc 55-65µ, 80A $402 \\5 \\5 Ordering Information IRIS N 96-Well Plates Ordering Information IRIS MCX 96-Well Plates Part No. Description Qty Price Part No. Description Qty Price IR96441 IRIS N 96 Well 10mg/1mL 25-35µ \\1 $308 IR96541 IRIS MCX 96 Well 10mg/1mL 25-35µ \\1 $308 IR96442 IRIS N 96 Well 25mg/1mL 25-35µ \\1 $319 IR96542 IRIS MCX 96 Well 25mg/1mL 25-35µ \\1 $319 IR96443 IRIS N 96 Well 30mg/1mL 25-35µ \\1 $325 IR96543 IRIS MCX 96 Well 30mg/1mL 25-35µ \\1 $325 IR96444 IRIS N 96 Well 50mg/1mL 25-35µ \\1 $336 IR96544 IRIS MCX 96 Well 50mg/1mL 25-35µ \\1 $336 IR96445 IRIS N 96 Well 60mg/1mL 25-35µ \\1 $352 IR96545 IRIS MCX 96 Well 60mg/1mL 25-35µ \\1 $352 IR96446 IRIS N 96 Well 25mg/2mL 25-35µ \\1 $341 IR96546 IRIS MCX 96 Well 25mg/2mL 25-35µ \\1 $341 IR96447 IRIS N 96 Well 30mg/2mL 25-35µ \\1 $352 IR96547 IRIS MCX 96 Well 30mg/2mL 25-35µ \\1 $352 IR96448 IRIS N 96 Well 60mg/2mL 25-35µ \\1 $363 IR96548 IRIS MCX 96 Well 60mg/2mL 25-35µ \\1 $363 IR96449 IRIS N 96 Well 10mg/1mL 55-65µ, 80A \\1 $308 IR96549 IRIS MCX 96 Well 10mg/1mL 55-65µ, 80A \\1 $308 IR96450 IRIS N 96 Well 25mg/1mL 55-65µ, 80A \\1 $319 IR96550 IRIS MCX 96 Well 25mg/1mL 55-65µ, 80A \\1 $319 IR96451 IRIS N 96 Well 30mg/1mL 55-65µ, 80A \\1 $325 IR96551 IRIS MCX 96 Well 30mg/1mL 55-65µ, 80A \\1 $325 IR96452 IRIS N 96 Well 50mg/1mL 55-65µ, 80A \\1 $336 IR96552 IRIS MCX 96 Well 50mg/1mL 55-65µ, 80A \\1 $336 IR96453 IRIS N 96 Well 60mg/1mL 55-65µ, 80A \\1 $352 IR96553 IRIS MCX 96 Well 60mg/1mL 55-65µ, 80A \\1 $352 IR96454 IRIS N 96 Well 25mg/2mL 55-65µ, 80A \\1 $341 IR96554 IRIS MCX 96 Well 25mg/2mL 55-65µ, 80A \\1 $341 IR96455 IRIS N 96 Well 30mg/2mL 55-65µ, 80A \\1 $352 IR96555 IRIS MCX 96 Well 30mg/2mL 55-65µ, 80A \\1 $352 IR96456 IRIS N 96 Well 60mg/2mL 55-65µ, 80A \\1 $363 IR96556 IRIS MCX 96 Well 60mg/2mL 55-65µ, 80A \\1 $363 430 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

IRIS SPE Ordering Information Ordering Information IRIS MAX SPE Cartridges IRIS PLUS SPE Cartridges Part No. Description Qty Price Part No. Description Qty Price (Box) (Box) IR64247 IRIS MAX 60mg/6.0mL 25-35µ \\30 $213 $158 IR64262 IRIS MAX 50mg/1.0mL 25-35µ \\100 $268 IR34247 IRIS PLUS 60mg/6.0mL 25-35µ \\30 $228 IR64263 IRIS MAX 100mg/1.0mL 25-35µ \\100 $281 $226 IR64264 IRIS MAX 100mg/3mL 25-35µ \\100 $281 IR34262 IRIS PLUS 50mg/1.0mL 25-35µ \\100 $253 IR64265 IRIS MAX 200mg/3.0mL 25-35µ \\100 $292 $264 IR64266 IRIS MAX 500mg/6.0mL 25-35µ \\30 $226 IR34263 IRIS PLUS 100mg/1.0mL 25-35µ \\100 $215 IR64444 IRIS MAX 60mg/1.0mL 25-35µ \\100 $270 $215 IR64445 IRIS MAX 200mg/6.0mL 25-35µ \\30 $204 IR34264 IRIS PLUS 100mg/3.0mL 25-35µ \\100 $171 IR64446 IRIS MAX 60mg/3.0mL 25-35µ \\100 $275 $248 IR64453 IRIS MAX 500mg/3.0mL 25-35µ \\50 $325 IR34265 IRIS PLUS 200mg/3.0mL 25-35µ \\100 $297 IR64460 IRIS MAX 10mg/1.0mL 25-35µ \\100 $193 $160 IR64461 IRIS MAX 30mg/1.0mL 25-35µ \\100 $204 IR34266 IRIS PLUS 500mg/6.0mL 25-35µm \\30 $171 IR64462 IRIS MAX 30mg/3.0mL 25-35µ \\100 $275 $248 IR64467 IRIS MAX 1000mg/60.0mL 25-35µ \\10 $138 IR34444 IRIS PLUS 60mg/1.0mL 25-35µ \\100 $132 IR64468 IRIS MAX 10mg/1.0cc 55-65µ, 80A \\100 $193 $160 IR64469 IRIS MAX 30mg/1.0cc 55-65µ, 80A \\100 $204 IR34445 IRIS PLUS 200mg/6.0mL 25-35µ \\30 $171 IR64470 IRIS MAX 60mg/1.0cc 55-65µ, 80A \\100 $270 $215 IR64471 IRIS MAX 100mg/1.0cc 55-65µ, 80A \\100 $281 IR34446 IRIS PLUS 60mg/3.0mL 25-35µ \\100 $226 IR64472 IRIS MAX 60mg/3.0cc 55-65µ, 80A \\100 $275 $248 IR64473 IRIS MAX 200mg/3.0cc 55-65µ, 80A \\100 $292 IR34453 IRIS PLUS 500mg/3.0mL 25-35µ \\50 $297 IR64474 IRIS MAX 500mg/3.0cc 55-65µ, 80A \\50 $325 $171 IR64475 IRIS MAX 200mg/6.0cc 55-65µ, 80A \\30 $204 IR34460 IRIS PLUS 10mg/1.0mL 25-35µ \\100 $215 IR64476 IRIS MAX 500mg/6.0cc 55-65µ, 80A \\30 $226 $132 IR64477 IRIS MAX 1000mg/60.0cc 55-65µ, 80A \\10 $138 IR34461 IRIS PLUS 30mg/1.0mL 25-35µ \\100 $385 IR64478 IRIS MAX 10000mg/60.0cc 55-65µ, 80A $402 IR64479 IRIS MAX 200mg/10mL 25-35µ, 80A \\5 $248 IR34462 IRIS PLUS 30mg/3.0mL 25-35µ \\100 \\30 IR34467 IRIS PLUS 1000mg/60.0mL 25-35µ \\10 IR34468 IRIS PLUS 10mg/1.0cc 55-65µ, 80A \\100 IR34469 IRIS PLUS 30mg/1.0cc 55-65µ, 80A \\100 IR34470 IRIS PLUS 60mg/1.0cc 55-65µ, 80A \\100 IR34471 IRIS PLUS 100mg/1.0cc 55-65µ, 80A \\100 IR34472 IRIS PLUS 60mg/3.0cc 55-65µ, 80A \\100 IR34474 IRIS PLUS 500mg/3.0cc 55-65µ, 80A \\50 IR34475 IRIS PLUS 200mg/6.0cc 55-65µ, 80A \\30 IR34476 IRIS PLUS 500mg/6.0cc 55-65µ, 80A /30 IR34477 IRIS PLUS 1000mg/60.0cc 55-65µ, 80A \\10 IR34478 IRIS PLUS 10000mg/60.0cc 55-65µ, 80A \\5 Ordering Information Ordering Information IRIS PLUS 96-Well Plates IRIS MAX 96-Well Plates Part No. Description Qty Price Part No. Description Qty Price IR96641 IRIS MAX 96 Well 10mg/1mL 25-35µ \\1 $308 IR96341 IRIS PLUS 96 Well 10mg/1mL 25-35µ \\1 $303 IR96642 IRIS MAX 96 Well 25mg/1mL 25-35µ \\1 $319 IR96342 IRIS PLUS 96 Well 25mg/1mL 25-35µ \\1 $314 IR96643 IRIS MAX 96 Well 30mg/1mL 25-35µ \\1 $325 IR96343 IRIS PLUS 96 Well 30mg/1mL 25-35µ \\1 $319 IR96644 IRIS MAX 96 Well 50mg/1mL 25-35µ \\1 $336 IR96344 IRIS PLUS 96 Well 50mg/1mL 25-35µ \\1 $330 IR96645 IRIS MAX 96 Well 60mg/1mL 25-35µ \\1 $352 IR96345 IRIS PLUS 96 Well 60mg/1mL 25-35µ \\1 $347 IR96646 IRIS MAX 96 Well 25mg/2mL 25-35µ \\1 $341 IR96346 IRIS PLUS 96 Well 25mg/2mL 25-35µ \\1 $330 IR96647 IRIS MAX 96 Well 30mg/2mL 25-35µ \\1 $352 IR96347 IRIS PLUS 96 Well 30mg/2mL 25-35µ \\1 $347 IR96648 IRIS MAX 96 Well 60mg/2mL 25-35µ \\1 $363 IR96348 IRIS PLUS 96 Well 60mg/2mL 25-35µ \\1 $358 IR96649 IRIS MAX 96 Well 10mg/1mL 55-65µ, 80A \\1 $308 IR96349 IRIS PLUS 96 Well 10mg/1mL 25-35µ \\1 $303 IR96650 IRIS MAX 96 Well 25mg/1mL 55-65µ, 80A \\1 $319 IR96350 IRIS PLUS 96 Well 25mg/1mL 25-35µ \\1 $314 IR96651 IRIS MAX 96 Well 30mg/1mL 55-65µ, 80A \\1 $325 IR96351 IRIS PLUS 96 Well 30mg/1mL 25-35µ \\1 $319 IR96652 IRIS MAX 96 Well 50mg/1mL 55-65µ, 80A \\1 $336 IR96352 IRIS PLUS 96 Well 50mg/1mL 25-35µ \\1 $330 IR96653 IRIS MAX 96 Well 60mg/1mL 55-65µ, 80A \\1 $352 IR96353 IRIS PLUS 96 Well 60mg/1mL 25-35µ \\1 $347 IR96654 IRIS MAX 96 Well 25mg/2mL 55-65µ, 80A \\1 $341 IR96354 IRIS PLUS 96 Well 25mg/2mL 25-35µ \\1 $330 IR96655 IRIS MAX 96 Well 30mg/2mL 55-65µ, 80A \\1 $352 IR96355 IRIS PLUS 96 Well 30mg/2mL 25-35µ \\1 $347 IR96656 IRIS MAX 96 Well 60mg/2mL 55-65µ, 80A \\1 $363 IR96356 IRIS PLUS 96 Well 60mg/2mL 25-35µ \\1 $358 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 431

IRIS SPE Ordering Information Ordering Information IRIS WAX SPE Cartridges IRIS WCX SPE Cartridges Part No. Description Qty Price Part No. Description Qty Price (Box) (Box) IR86011 IRIS WAX 10mg/1.0mL 25-35µ $193 IR84012 IRIS WCX 10mg/1.0mL 25-35µ \\100 $193 IR86012 IRIS WAX 30mg/1.0mL 25-35µ \\100 $204 IR84013 IRIS WCX 30mg/1.0mL 25-35µ \\100 $204 IR86013 IRIS WAX 60mg/1.0mL 25-35µ \\100 $270 IR84014 IRIS WCX 60mg/1.0mL 25-35µ \\100 $270 IR86014 IRIS WAX 100mg/1mL 25-35µ \\100 $281 IR84015 IRIS WCX 100mg/1mL 25-35µ \\100 $281 IR86015 IRIS WAX 60mg/3mL 25-35µ \\100 $275 IR84016 IRIS WCX 60mg/3mL 25-35µ \\100 $275 IR86016 IRIS WAX 200mg/3mL 25-35µ \\100 $292 IR84017 IRIS WCX 200mg/3mL 25-35µ \\100 $292 IR86017 IRIS WAX 500mg/3mL 25-35µ \\100 $325 IR84018 IRIS WCX 500mg/3mL 25-35µ \\50 $325 IR86018 IRIS WAX 200mg/6mL 25-35µ \\50 $204 IR84019 IRIS WCX 200mg/6mL 25-35µ \\30 $204 IR86019 IRIS WAX 500mg/6mL 25-35µ \\30 $226 IR84020 IRIS WCX 500mg/6mL 25-35µ \\30 $226 IR86020 IRIS WAX 1000mg/60mL 25-35µ \\30 $138 IR84021 IRIS WCX 1000mg/60mL 25-35µ \\10 $138 IR86021 IRIS WAX 10000mg/60mL 25-35µ \\10 $402 IR84022 IRIS WCX 10000mg/60mL 25-35µ $402 IR86000 IRIS WAX 10mg/1.0cc 55-65µ, 80A $193 IR84001 IRIS WCX 10mg/1.0cc 55-65µ, 80A \\5 $193 IR86001 IRIS WAX 30mg/1.0cc 55-65µ, 80A \\5 $204 IR84002 IRIS WCX 30mg/1.0cc 55-65µ, 80A \\100 $204 IR86002 IRIS WAX 60mg/1.0cc 55-65µ, 80A \\100 $270 IR84003 IRIS WCX 60mg/1.0cc 55-65µ, 80A \\100 $270 IR86003 IRIS WAX 100mg/1.0cc 55-65µ, 80A \\100 $281 IR84004 IRIS WCX 100mg/1.0cc 55-65µ, 80A \\100 $281 IR86004 IRIS WAX 60mg/3.0cc 55-65µ, 80A \\100 $275 IR84005 IRIS WCX 60mg/3.0cc 55-65µ, 80A \\100 $275 IR86005 IRIS WAX 200mg/3.0cc 55-65µ, 80A \\100 $292 IR84006 IRIS WCX 200mg/3.0cc 55-65µ, 80A \\100 $292 IR86006 IRIS WAX 500mg/3.0cc 55-65µ, 80A \\100 $325 IR84007 IRIS WCX 500mg/3.0cc 55-65µ, 80A \\100 $325 IR86007 IRIS WAX 200mg/6.0cc 55-65µ, 80A \\100 $204 IR84008 IRIS WCX 200mg/6.0cc 55-65µ, 80A \\50 $204 IR86008 IRIS WAX 500mg/6.0cc 55-65µ, 80A \\50 $226 IR84009 IRIS WCX 500mg/6.0cc 55-65µ, 80A \\30 $226 IR86009 IRIS WAX 1000mg/60mL 25-35µ \\30 $138 IR84010 IRIS WCX 1000mg/60mL 25-35µ \\30 $138 IR86010 IRIS WAX 10000mg/60.0cc 55-65µ, 80A \\30 $402 IR84011 IRIS WCX 10000mg/60.0cc 55-65µ, 80A \\10 $402 \\10 \\5 \\5 Ordering Information Ordering Information IRIS WAX 96-Well Plates IRIS WCX 96-Well Plates Part No. Description Qty Price Part No. Description Qty Price IR97508 IRIS WAX 96 Well 10mg/1mL 25-35µ \\1 $308 IR97008 IRIS WCX 96 Well 10mg/1mL 25-35µ \\1 $308 IR97509 IRIS WAX 96 Well 25mg/1mL 25-35µ \\1 $319 IR97510 IRIS WAX 96 Well 30mg/1mL 25-35µ \\1 $325 IR97009 IRIS WCX 96 Well 25mg/1mL 25-35µ \\1 $319 IR97511 IRIS WAX 96 Well 50mg/1mL 25-35µ \\1 $336 IR97512 IRIS WAX 96 Well 60mg/1mL 25-35µ \\1 $352 IR97010 IRIS WCX 96 Well 30mg/1mL 25-35µ \\1 $325 IR97513 IRIS WAX 96 Well 25mg/2mL 25-35µ \\1 $341 IR97514 IRIS WAX 96 Well 30mg/2mL 25-35µ \\1 $352 IR97011 IRIS WCX 96 Well 50mg/1mL 25-35µ \\1 $336 IR97515 IRIS WAX 96 Well 60mg/2mL 25-35µ \\1 $363 IR97500 IRIS WAX 96 Well 10mg/1mL 55-65µ, 80A \\1 $308 IR97012 IRIS WCX 96 Well 60mg/1mL 25-35µ \\1 $352 IR97501 IRIS MAX 96 Well 25mg/1mL 55-65µ, 80A \\1 $319 IR97502 IRIS MAX 96 Well 30mg/1mL 55-65µ, 80A \\1 $325 IR97013 IRIS WCX 96 Well 25mg/2mL 25-35µ \\1 $341 IR97503 IRIS MAX 96 Well 50mg/1mL 55-65µ, 80A \\1 $336 IR97504 IRIS MAX 96 Well 60mg/1mL 55-65µ, 80A \\1 $352 IR97014 IRIS WCX 96 Well 30mg/2mL 25-35µ \\1 $352 IR97505 IRIS MAX 96 Well 25mg/2mL 55-65µ, 80A \\1 $341 IR97506 IRIS MAX 96 Well 30mg/2mL 55-65µ, 80A \\1 $352 IR97015 IRIS WCX 96 Well 60mg/2mL 25-35µ \\1 $363 IR97507 IRIS MAX 96 Well 60mg/2mL 55-65µ, 80A \\1 $363 IR97000 IRIS WCX 96 Well 10mg/1mL 55-65µ, 80A \\1 $308 IR97001 IRIS WCX 96 Well 25mg/1mL 55-65µ, 80A \\1 $319 IR97002 IRIS WCX 96 Well 30mg/1mL 55-65µ, 80A \\1 $325 IR97003 IRIS WCX 96 Well 50mg/1mL 55-65µ, 80A \\1 $336 IR97004 IRIS WCX 96 Well 60mg/1mL 55-65µ, 80A \\1 $352 IR97005 IRIS WCX 96 Well 25mg/2mL 55-65µ, 80A \\1 $341 IR97006 IRIS WCX 96 Well 30mg/2mL 55-65µ, 80A \\1 $352 IR97007 IRIS WCX 96 Well 60mg/2mL 55-65µ, 80A \\1 $363 432 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

CEREX® - Polycrom SPE and Trace-B® SPE ® TM CEREX® Polycrom CEREX Polycrom SPE columns are produced from a family of polymeric sorbents. These sor- CEREX - POLYCROM bents are divided into two classes, namely, (1) co-polymeric and (2) functionalized polymers. Polymeric columns Co-polymers are the result of polymerizing two monomers, for solid phase extraction such as styrene and divinylbenzene. By choosing different monomers, the polarity of the resulting co-polymer can be • CEREX® columns contain microparticulate sorbents which varied thereby providing a polarity spectrum of sorbents, allow for much smaller bed volumes compared to particularly useful for solid phase extraction applications. conventional SPE columns Polycrom functionalized sorbents are modified, highly cross- linked divinylbenzene polymers. These modifications afford • All CEREX® format columns are constructed of proprietary, extraordinary properties when used for solid phase extraction. ultra-clean polypropylene stock with borosilicate glass For example, functionalizing DVB with a quaternarized amine fabric retainers produces a strong anion exchanger. This ion exchanger will perform in mixed mode, a strong anion exchanger coupled to • CEREX® provides low column volume thereby requiring the hydrophobic interaction provided by the divinylbenzene low overall solvent usage backbone. • The retainer system used in CEREX® utilizes hydrophilic borosilicate fabric which provides low resistance to flow • CEREX® retainers have the added advantage of filtration capability Ordering Information CEREX ® PolycromTM columns - Application Specific Polymers Part No. Description Qty Price (Box) SW682-0353C Polycrom THC, 3cc, 35mg 100 $ 320.00 SW682-0353 Polycrom THC, 3cc, 35mg 500 $1278.00 SW682-0503 Cerex Polycrom THC, 3cc, 50ml 500 $1278.00 SW682-0506C Polycrom THC, 6cc Columns, 50mg 100 $ 345.00 SW682-0506 Polycrom THC, 50mg 6cc 500 $1342.00 SW691-0353C Polycrom Clin II, 3cc, 35mg 100 $ 294.00 SW691-0353C-HC Polycrom Clin II, 3cc, 35mg, HC Filter 100 $ 337.00 SW691-0353 Polycrom Clin II, 3cc, 35mg 500 $1176.00 SW691-0353M-HC Polycrom Clin II, 3cc, 35mg, HC Filter, 1000 columns/box 1,000 $2588.00 SW691-0506C Polycrom Clin II, 6cc, 50mg 100 $ 345.00 SW691-0506 Polycrom Clin II, 50mg, 6ml 500 $1342.00 Trace-B® Columns • True dual mode column - hydrophilic polymer with a cation exchanger High efficiency hydrophilic DVB polymer • Cerex Format—eliminates column channeling for high for extracting drugs of abuse from speed processing with flow rates up to 10 ml/min biological matrixes • Hydrophilic polymer - no column conditioning required, eliminates column drying problem • 10-15µ particle size - provides high recoveries of drugs at • High cross linking - provides a rigid polymer that is stable very low concentrations from pH 1 to 14 and eliminates troublesome pH adjustment steps in extraction process • 200Å pore size - high efficiency sorbent allows for even flow with high recovery as well as elution in small volumes • Polymer based chemistry - Excellent lot-to-lot reproducibility - Single source manufacturing Ordering Information Trace-B ® PolycromTM columns - Application Specific Polymers Part No. Description Qty Price (Box) SWTN-315C Trace-N, 15mg, 10 micron 100 $ 250.00 SWTN-315M Trace-N, 15mg, 10 micron 1,000 $2173.00 SWTB-335C Trace-B, Extraction Column, 35mg, 3cc 100 $ 320.00 SWTB-335C-HC Trace-B, 35mg, 3cc, HC Filter 100 $ 337.00 SWTB-335M Trace-B, TB-335M, 35mg, 3cc 1,000 $2173.00 SWTB-335M-HC Trace-B, 35mg, 3cc, HC Filter 1,000 $2289.00 SWTB-0506C Trace-B Extraction Columns, 50mg, 6cc 100 $ 307.00 SWTB-0506M Trace-B, 50mg, 6cc 1,000 $2556.00 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 433

InnoSep™ Vacuum Manifolds Vacuum manifolds for SPE sample preparation, filtration, and Place the disposable liner into the glass vacuum chamber, elusion are available in 12, 16, and 24 port configurations from and replace the manifold lid. Ciro Manufacturing Corp. These manifolds permit consistent Proceed with all conditioning and sample preparation steps. extraction and filtration results. Multiple sample processing Just prior to final elution, the liner, containing the waste with these manifolds simplifies procedures and saves solvents, is removed from the vacuum chamber. There are time.The manifolds consist of a clear glass chamber and lid to small handles at each end of the waste liner to facilitate its which a vacuum is applied to draw a sample through an SPE removal. Replace the lid, and proceed with the final elution of column, cartridge, or disk. the analyte. Waste solvents should be properly discarded from the liner. Adjustable racks placed in the glass vacuum chamber will The liner can be cleaned and re-used a number of times, prior accommodate a variety of sample collection vessels, to discarding. including test tubes, autosampler vials, volumetric flasks, and Erlenmeyer flasks. Eluants are deposited directly into the Ordering Information - collection vessel of choice via polypropylene, optional Vacuum Manifolds, 12 Position stainless steel, or Teflon needles. Part No. Description Qty Price Drying attachments for the 12 and 24 port manifolds will direct a flow of air or nitrogen into the collection vessels to dry CM4012 Vacuum manifold Set-Complete 1 $620 eluants prior to further analysis. Drying attachments can also be connected, via adapters, to SPE columns or cartridges in CM4013 Glass chamber 1 $118 order to dry the column or cartridge prior to final elusion. Optional disposable solvent resistant polypropylene liners are CM4014 Cover, gasket, & 12 stopcocks 1 $255 available for the twelve port manifolds.These waste liners greatly simplify sample preparation, solvent disposal, and CM4015 Gaskets 2 $ 14 clean-up. CM4016 Vacuum gauge, valve, & glass chamber 1 $395 Drying attachments CM4017 Needles - Polypropylene 12 $ 26 Drying Attachments are available for the 12 and 24 port manifolds, which CM4018 Needles - Stainless Steel 12 $ 56 will direct the flow of air or nitrogen into the collection vessels to concentrate CM4019 Collection Rack-shelves, legs, 1 $ 63 eluants, prior to further analysis. Drying clips, & posts attachments can also be connected directly to columns, cartridges, or disks, CM4020 Plate - 13mm 1 $ 54 via adapters to permit drying sorbent beds, prior to final elution. CM4021 Plate - volumetric flask 1 $ 54 Teflon needles and control valves CM4022 Plate - 16mm test tube 1 $ 54 Disposable Teflon needles and CM4023 Plate - autosampler vial 1 $ 54 Teflon needles with flow control valves are designed to fit through CM4024 Plate - dimple 1 $ 54 the manifold lid via the luer fitting.These needles deliver the CM4025 Plate - base 1 $ 54 eluant directly from the SPE column or cartridge into the collection vessel in the vacuum CM4026 Stopcocks 12 $ 48 chamber.These needles, when used in conjunction with Teflon columns and Teflon frits ensure zero extractables from the col- CM4027 Drying attachment 1 $324 umn, frits, and fluid path. This combination is especially use- ful for critical sample analysis, such as environmental sam- CM4028 12 Position PP vacuum waste container 10 $109 ples. Ordering Information - Waste containers Vacuum Manifolds, 16 Position Disposable solvent resistant polypropylene waste liners, for Part No. Description Qty Price 12 port manifolds, greatly simplify sample preparation, solvent disposal, and final clean-up of the manifold. CM4416 Vacuum manifold Set-Complete 1 $703 The disposable waste liner is a molded solvent resistant polypropylene liner that fits into the vacuum chamber of the 12 CM4417 Glass chamber 1 $153 port manifold. The liner is designed to contain all liquids used in SPE CM4418 Cover, gasket, & 12 stopcocks 1 $372 sample preparation.To use the liner, remove the manifold lid and take out the rack and shelf set. CM4419 Gaskets 2 $ 20 CM4420 Vacuum gauge, valve, & glass chamber 1 $452 CM4421 Needles - Polypropylene 16 $ 35 CM4422 Needles - Stainless Steel 16 $ 89 CM4423 Collection Rack-shelves, legs, 1 $146 clips, & posts CM4424 Plate - 13mm 1 $ 66 CM4426 Plate - 16mm test tube 1 $ 66 CM4428 Plate - dimple 1 $ 66 CM4429 Plate - base 1 $ 66 CM4430 Stopcocks 16 $ 63 CM4431 Drying attachment 1 $399 434 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

Vacuum Manifolds & Accessories Ordering Information - Ordering Information - Vacuum Manifolds, 24 Position Vacuum Manifold Accessories Part No. Description Price Part No. Description Qty Price Qty CM4102 Female Luer Fittings 2 $8 CM4824 Vacuum manifold Set-Complete 1 $834 CM4103 Male Luer Fittings 2 $8 CM4104 Support posts for rack 3 $ 59 CM4825 Glass chamber 1 $153 CM4105 Legs for cover - black 4 $ 19 CM4106 Vacuum gauge & valve assembly 1 $132 CM4826 Cover, gasket, & 12 stopcocks 1 $403 CM4107 Valve assembly only 1 $113 CM4108 Vacuum gauge 1 $ 33 CM4827 Gaskets 2 $ 20 CM4109 Retaining clips 12 $ 34 CM4110 Vacuum manifold plugs 50 $ 22 CM4828 Vacuum gauge, valve, & glass chamber 1 $568 CM4829 Needles - Polypropylene 24 $ 52 CM4830 Needles - Stainless Steel 24 $111 CM4831 Collection Rack-shelves, legs, 1 $146 clips, & posts CM4832 Plate - 13mm 1 $ 66 CM4834 Plate - 16mm test tube 1 $ 66 CM4836 Plate - dimple 1 $ 66 Ordering Information - Teflon Needles & Control Valves CM4837 Plate - base 1 $ 66 CM4838 Stopcocks 24 $ 95 CM4839 Drying attachment 1 $419 Part No. Description Qty Price CM4202 Control Valve 25 $149 CM4204 Control Valve 50 $298 CM4210 Teflon Needles 100 $124 CM4212 Teflon Needles 500 $620 Collection Tubes & 200 L / 3mg Waste Tubes Titansphere® Phos-TiO Kit 10 L / 1mg Tip column Easy to operate Ordering Information - The total number of process is only 4 steps and the operation Titansphere® Phos-TiO Kits time is only 40 minutes. High capacity Part No. Description Qty Price Optimized surface activity of Titansphere TiO particles to purify and enrich the phosphopeptides. GL5010-21310 Titansphere Phos-TiO Kit Tip 6mL 96 $1020 High selectivity 1mg/10µL Sol. A 6mL, Sol. B An enhancer is added to reduce the non-specific adsorption and selectively purifies and enriches phosphopeptides. GL5010-21311 Titansphere Phos-TiO Kit Tip 2mL 24 $ 292 High throughput 3mg/200µL Sol. A 2mL, Sol. B Requires 2 types of centrifuge adaptors (sold separately) during the operation. Small number of samples or large GL5010-21312 Titansphere Phos-TiO Kit Tip 6mL 96 $1020 number of samples using a 96-well format can be operated at 3mg/200µL Sol. A 6mL, Sol. B the same time. Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 435

Vacuum Manifolds & Accessories MicroLute™ Manifold The MicroLute™ vacuum manifold from Porvair Sciences is • Designed to take most filter plates manufactured to precision machined from crystal clear acrylic (top plate) and the SBS/ANSI specification with long drip directors acetal polymer (plenum chamber). The acrylic top plate allows visual access to the plenum chamber for checking progress of • Also designed so that SPE plates can be used, the separation process. especially the Porvair Sciences MicroLute™ plate Ordering Information • Fitted with valve controller to ensure accurate adjustments of vacuum to the manifold • On/off valve for speed of use • Square well collection plate volumes of 350µl, 1ml and 2ml may be used. Any plate up to 44mm in height can be used • Fitted with a custom O-ring in upper surface allowing airtight interface between plates during operation • Removable top plate to install reservoir tray or collection plate • Chamber has a medium resistance to alcohols and weak acids Part No. Description Qty Price 1 $1512.00 PV-228008 Standard MicroLute™ manifold to hold deep 96-well collection plate 1 $ 45.00 1 $ 45.00 PV-228007 Replacement gasket, profile (to fit between top plate and vacuum chamber), for PV-228008/PV-228020 1 $ 173.00 PV-228009 Replacement gasket, flat (to fit top plate below filtration plate), for PV-228008/PV-228020 1 $ 251.00 PV-228010 Optional spacer insert, polypropylene, to allow use of 1ml round well polypropylene microplates (PV- 219002) in acrylic deep well manifolds (PV-228008/PV-228020) 25 $ 107.00 1 $ 126.00 PV-228012 Optional spacer insert, HDPE, 350µl, to allow use of 350µl microplates in acrylic manifolds (PV-228008/PV-228020) PV-219010 Disposable reservoir tray, PVC PV-228001 Single plate manifold, high impact polystyrene, for filter plates (no waste collection) (not shown) Universal Robotic Manifold The Manifold is fully compatible with most commercial robotic liquid handling systems. Based on the successful MicroLute™ Acrylic Manifold, the new Universal Robotic Manifold is designed to be easily The Universal Robotic Manifold is suitable for a range of assembled and disassembled by robotic manipulators, manufacturerʼs plates without an adaptor. For certain plate enabling productive automation of SPE or DNA clean-up pro- types a Shallow Adaptor or a Deep Adaptor is required. cedures. Able to accommodate collection plates from 14mm - 44mm in height and adaptable for working with short, medium • Compatible with any filter plate type and long skirts and drip directors, the Universal Robotic • Robotic friendly designs aids automation Manifold can operate with many different brands of filter plate • Chemically resistant acetal/acrylic construction and collection trays. Supplied with an integral vacuum gauge, • Adaptable for different collection plates the Manifold provides complete control of vacuum pressure • Easy visual inspection of process ensuring plates are processed with high reproducibility. • Built in vacuum gauge for reproducibility Ordering Information Part No. Description Qty Price PV-228020 Universal robotic compatible manifold to hold deep 96-well collection plate 1 $1985.00 1 $ 390.00 PV-228021 Adaptor 1 for medium skirt/medium drip director plates 1 $ 390.00 PV-228022 Adaptor 2 for short skirt/long drip director plates Gaskets and spacers are identical to those for our standard MicroLute™ acrylic manifold 436 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

Positive Pressure Manifolds Cerex System 48 Pressure • Segmented manifold allows Processor II gas shut off to unused ports. The CEREX SYSTEM 48 PRESSURE PROCESSOR II has • Optional heated manifold all the capabilities of the original version with the following provides heated gas flow to added improvements. assist in column drying up to 50°C. • Pressure regulated rotometer allows for precise control of application flow down to 0.5ml/min. • Improved extraction selectivity achieves 100% drying of SPE • Max flow pressure to 100psi - the increased pressure column bed without the use capability allows for improved sorbent drying times by of bridge solvents. flowing nitrogen at higher pressures. • Fixed platform improves SPE colum handling Ordering Information and processing. Part No. Description Price $3884.00 SW289-0004 System 48 Pressure Processor II with 1 Waste Bin, 3 Waste Bin Inserts $1093.00 SW289-1000 Manifold Heater Option to 50°C $ 36.00 SW279-0009 Waste Bin ( 3 per pack) $ 36.00 SW279-0010 Gas Supply Adapter, includes 1/8\"-1/4\" NPT Fitting, 6ft. of 1/4\" Tubing $ 64.00 SW279-0012 48 Place Manifold Replacement Gasket $ 271.00 SW279-0015 Collection tube rack, 12 x 75mm tubes $ 271.00 SW279-0016 Collection Rack 13mm x 100MM 48-Place $ 271.00 SW279-0017 Collection Tube Rack 16 x 100mm. $ 271.00 SW279-0018 Collection Vial Rack 12 x 32mm Autosampler. $ 298.00 SW279-0020 Collection Rack 16 x125 10/ml, Centrifuge $ 271.00 SW279-0021 1ml SPE Column Rack $ 271.00 SW279-0022 SPE Rack, 3CC, For 48 Place Processor $ 271.00 SW279-0023 SPE Rack, 6CC for 48 Place Processor System 96 Pressure Processor II • Positive Pressure manifold provides uniform flow for all extraction steps. The System® 96 PRESSURE PROCESSOR II uses positive pressure for 96 well SPE plate processing. The processor • All 96 positions are individually produces the same flow rate in each of the 96 columns restricted to provide even arrayed in the 8 x 12 micro-plate pattern. The flow rate is pressure to each column. Even continuous, and response to flow rate adjustment is flow can be maintained if you immediate. are using one or all-96 positions. Ordering Information • Dual pressure regulators. Allows users to set 2 different pressures for extraction and column drying. • Uniform drying of extraction column bed is achieved by flowing nitrogen or air at 25 psi. Part No. Description Price SW288-0001 System 96 Processor II, includes 1ml x 96 Collection Tray, 2ml x 96 Collection Tray, $3969.00 10ml x 24 Waste Tray, 96 Place Sealing Gasket, Gas Supply Adapter SW278-0032 $ 18.00 SW278-0034 Cerex 96 Collection Plate 10ml x 24 $ 18.00 SW278-0038 1ml x 96 Collection Tray $ 18.00 SW278-0037 2ml x 96 Collection Tray $ 62.00 SW278-0035 96 Column Holder $ 52.00 SW279-0010 96 Place Sealing Gasket $ 36.00 SW288-1000 Gas Supply Adapter, includes 1/8\"-1/4\" NPT Fitting, 6ft. of 1/4\" Tubing $1093.00 System 96 II Heated Manifold Option View demo’s of the 48 & 96 PPM’s in action at www.speware.com Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 437

Solvent Evaporators Porvair evaporators are designed to remove the traditional UltraVap™ laboratory ʻbottleneckʼ of solvent evaporation from microplates prior to analysis or reconstitution in storage buffer. • Designed for heat sensitive and thermally These evaporators give significant throughput advantages to labile samples laboratories looking to optimize microplate sample preparation productivity. • Nine different drying programmes can be retained Faster than centrifugal evaporation, significant increases in in the memory sample throughput are achieved through advanced evaporator head technology and an innovative manifold design, which • Ability to upload and download methods directly injects heated nitrogen into each individual well of the • Robotic plate stage automatically moves plates microplate simultaneously. The evaporators have been designed to be simple to install, up and down operate and maintain. Installation requires only connection to a • RS232 allows remote control via PC or LH robot gas supply or cylinder and mains electricity. Safety of operation • Removes most stubborn solvents in just minutes is ensured as the CE marked compact units fit into all fume • Can evaporate 500µl methanol in 5 minutes cupboards. Not suitable for high boiling solvents such as DMSO • Compact footprint allows the Ultravap to fit into all and water. standard fume cupboards • Choice of straight, curved & 24, 96 or 384 needles • Compatible with Tecan™, Hamilton™ and Beckman™ robotic liquid handlers Both MiniVap™ and UltraVap™ may be operated with a supply Whereas the MiniVap™ is a fully manual system, the Ultravap™ of clean, dry compressed air in place of nitrogen, if the chemistry is a digitally controlled programmable evaporator. It can be used allows. An in-line gas filter must be used in this case. as a stand alone unit or driven by a computer, often connected to a robotic liquid handler. After empirical data for dry down has MiniVap™ been produced, this can be entered into the memory of the Ultravap for future recall and use. Each programme has three • Quicker dry down times than standard methods such stages, with control of gas as vacuum oven temperature, pressure and plate height at each • Tests have shown that the MiniVap can evaporate stage. Nine different 500µl of methanol in less than 6 minutes methods can be perma- nently stored in memory. • Designed for any SBS/ANSI 96-well plate The Ultravap will then • 24 vial head now available evaporate plate contents • Simple to install and operate quickly, by keeping the • Easy adjustments of temperature, gas flow rates and heated gas needles close to the liquid surface at all needle depth into the wells times in the concentration • Compact footprint –fits all standard fume cupboards process, without further manual intervention. The MiniVap is purpose designed for low usage research and development departments where low numbers of individual Spare parts and consumables for evaporators also available. plates or vials need drying. The MiniVap is simple to operate and Please contact us. maintain. Installation requires only connection to a gas supply and standard mains socket. With manual control of the needle depth, gas temperature and flow rate, it allows fine control and quicker drying times than other standard methods. Ordering Information Part No. Description Price MiniVap™ PV-229201 MiniVap™ (230 Volts), with 96 spiral needles $ 12,850.00 PV-229200 MiniVap™ (230 Volts), with 96 straight needles $ 12,574.00 PV-229205 MiniVap™ (230 Volts), with 24 straight needles $ 12,574.00 PV-229203 MiniVap™ (110 Volts), with 96 spiral needles $ 12,850.00 PV-229202 MiniVap™ (110 Volts), with 96 straight needles $ 12,574.00 PV-229204 MiniVap™ (110 Volts), with 24 straight needles $ 12,574.00 UltraVap™ PV-229138 Ultravap™ 24 Straight needles, 230V, for 24-well plates and vial carriers $ 18,542.00 PV-229070 Ultravap™ 96 with spiral needles, 230V, for 96-well plates $ 19,354.00 PV-229135 Ultravap™ 96 with straight needles, 230V, for 96-well plates $ 18,542.00 PV-229071 Ultravap™ 384 with straight needles, 230V, for 384-well plates $ 19,872.00 PV-229139 Ultravap™ 24 Straight needles, 110V, for 24-well plates and vial carriers $ 18,542.00 PV-229080 Ultravap™ 96 with spiral needles, 110V, for 96-well plates $ 19,354.00 PV-229136 Ultravap™ 96 with straight needles, 110V, for 96-well plates $ 18,542.00 PV-229081 Ultravap™ 384 with straight needles, 110V, for 384-well plates $ 19,872.00 438 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

P3 Protein Precipitation Plates P3 Protein Precipitation Plates P3 is now commonly used as the protein precipitation plate of choice in many major pharmaceutical companies. • Dual frit design Optional drain cap and top cap mats are available for those • Pre-filter frit at 100µm traps large flocculant particles wishing to use vortex sample mixing. • Secondary frit traps fine protein particles at <10µm • Frits are hydrophobic/oleophobic. Ordering Information - Protein Precipitation Plates This retains sample/acetonitrile in the well to allow precipitation of the proteins until vacuum is applied. Part No. Description Qty Price • Pore size optimized to allow ideal flow rate • Inert filter material to ensure no adsorption of sample PV-240100 P3 Protein Precipitation Plate 1 $ 99 components PV-240200 P3 Protein Precipitation Plate 25 $337 • Frit structure prevents break through of PV-240010 Protein MicroLute™ High Efficiency* 1 $ 89 protein particles • Industry standard MicroLute™ format enables *The Protein MicroLute High Efficiency plate is exactly the same as the P3 but easy automation with the frits untreated. This plate is used when sample and acetronitrile are • Specially selected polypropylene for low extractables pre-mixed before being pipetted into the plate. Biological samples commonly contain proteins that interfere with downstream applications. The P3 plate uses the CRASH method, in which the protein is denatured with acetronitrile and the flocculant filtered out, allowing 96 samples to be handled at one time. The Protein Precipitation Plate, P3, is based on the industry standard MicroLute™ format, but with- out the chromatographic sorbent. Protein ʻcrashesʼ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up. The novel dual frit, hydrophobically treated matrix means that there is no ʻwetting outʼ and leakage of the sample through the plate before the application of vacuum. MicroLute™ BioVyon Co-sinter products BioVyon™ Co-sinter products are made using a solid-state media created from an ultra-pure and highly modified polymeric material with the lowest levels of extractables and leachables. Our proprietary BioVyon™ technology allows high-purity silica resins to be supported in a matrix which provides a high surface area whilst reducing channelling through the column. As the porous material is co-sintered under high pressure and temperature with the BioVyon™ polymer matrix, the resultant frit or column is impervious to further compression and easily resists channelling of liquids, even at high flow rates. BioVyon C8 and C18 products Porvair Sciences have introduced MicroLute™ BioVyon™ Co-sintered C8 and C18 silica SPE 96-well plates for low volume assays. Offered in 96-well MicroLute™ plates as a 10mg per well loading of C8 or C18 suitable for low sample volume bioassay preps and clean ups. Ordering Information - MicroLute™ BiVyon Co-sinter Products Micro structure of Part No. Description Qty Price BioVyon substrate PV-240030 C8 96-well plate 10mg per well 1 $319 PV-240031 C18 96-well plate 10mg per well 1 $319 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 439

Filtration Filter Membrane Compatibility Chart Filter Membrane Chemical Compatibility Chart Filter Membrane Chemical Compatibility Chart Nylon PTFE PVDF Regenerated Cellulose Nylon PTFE PVDF Regenerated Cellulose Chemical Chemical Acids LCCC Ketones CCNC LCCC CCNC Acetic, 25% NCCC Acetone C C N— Acetic, Glacial LCCC Cyclohexanone CC LC Formic, 25% NNCC Isopropylacetone CCNC Hydrochloric, 25% NCCC Methyl Ethyl Ketone Hydrochloric, Concentrated NC L L Methyl Isobutyl Ketone (MIBK) L L LC Sulfuric, 25% NC LC Esters CCCC Sulfuric, Concentrated NC LN 2-Ethoxyethyl Acetate C C—C Nitric, 25% NCCC Amyl Acetate CCCC Nitric, Concentrated LCCC Benzyl Benzoate CCCC Phosphoric, 25% Butyl Acetate C C—C Trichloroacetic, 10% CC L— Ethyl Acetate LCLC Bases C C C— Isopropyl Myristate LNCC Ammonium Hydroxide, 25% Methyl Acetate L C—C Sodium Hydroxide, 3 Normal CCCC Propyl Acetate CCCC Alcohols CCCC Propylene Glycol Acetate —C—C Amyl Alcohol CCCC Methyl Cellosolve Acetate Benzyl Alcohol CCCC Tricresyl Phosphate CCCC Butyl Alcohol CCCC Oxides — Ethers LCCC Ethanol, 70% CCCC Acetonitrile (Methyl Cyanide) CCCC Ethanol, 98% CCCC Aniline CCN L Ethylene Glycol CCCC Diethyl Acetamide LCC L Glycerine (Glycerol) CCCC Dimethyl Formamide CC L L Isopropanol CCCC Dimethyl Sulfoxide (DMSO) CCCC Methanol, 98% CCCC Dioxane C C N— n-Propanol Ethyl Ether CC LC Propylene Glycol CCCC Isopropyl Ether Hydrocarbons CCCC Pyridine CC L L Benzene CCCC Solvents with Nitrogen CCCC Hexane, Xylene C C C— Tetrahydrofuran Kerosene, Gasoline CCCC Triethanolamine CCC L Tetralin, Decalin Miscellaneous NCCC Toluene CCCC Formaldehyde Solution, 30% NC LC Halogenated Hydrocarbons CCCC Hydrogen Peroxide, 30% CCCC Carbon Tetrachloride LCCC Phenol, Aqueous, 10% Chlorobenzene (Mono) CCCC Silicone Oil and Mineral Oil Chloroform LCCC Freon CCCC Methylene Chloride CCCC Trichloroethane Trichloroethylene LEGEND - C=Compatible, L=Limited Compatibility, N=Not Compatible, =No Data Available, PTFE=Teflon®, PVDF=Polyvinyledene Difloride 440 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

Filtration Plates Filtration Plates Filter bottom microplate application guide • Polystyrene and polypropylene filter plates Solvent resistant Application Filter Drip detector Vol (µl/well) • Long and short drip directors Short 300 • Each well has an individual drainage spout ensuring Low Biomolecule Binding YAC (Yeast Cloning Assays) Hydrophilic 400 Low Biomolecule Binding DNA extraction from agarose gel PVDF 0.45µm Long 800 100% sample transfer Dye Terminator Clean Up • Manufactured from ultra pure grade polymer Sequencing Reaction Clean Up UHMW PE Short 300 • Standard ANSI/SBS footprint DNA/RNA Purification 25µm Long 400 • Robot friendly Ni-Nta 800 • Fit standard vacuum manifold Affinity Bead Streptavidin/biotin bead 300 Filtration plates are used in their simplest form to remove partic- Chromatography beads/resins 400 ulate matter from liquid. Either the particulate matter or the filtrate Purified cloned receptors 800 is needed for further study. Cell Based Receptor Binding 300 Signal transduction 400 Porvair Sciences has a range of filter plates to suit most filtration Phosphodiesterase 800 applications. The company has optimized filter plates for appli- Protein or nucleic acid desalt 2000 cations including cell harvesting, DNA separations, binding Cell membrane 2000 studies, plasmid isolation, general filtration and sample clean up. Neonatal Screening 5000 Porvair has a full range of 48-, 96- and 384-well microplates with Vesicle Assay 300 a choice of glass fibre, nylon, PVDF or polyethylene filtration Dye Terminator Removal 400 materials and well volumes ranging from 350µl to 5ml. Dye Terminator Clean Up 800 Sequencing Reaction Clean Up 300 Low Biomolecule Binding Solid Phase Extraction (SPE) P E Frit 25µm Long 400 Low Biomolecule Binding Short 800 YAC (Yeast Cloning Assays) Polypropylene 2000 High Biomolecule Binding Bacterial DNA prep for PCR 0.45µm Alumina 140 Cell fragments/membranes Long 400 Whole Cells 800 Protease assays Glass fibre Long 2000 Phosphodiesterase 0.7µm 300 Antibody neutralization 400 Mammalian Cells Capture 800 Calcium uptake Dual assay: Ca + receptor 300 Microsomes 400 Solid Phase Radioimmunoassays 800 DNA Binding Lysate Clarification 140 300 High Biomolecule Binding Cell homogenates. crude Glass fibre Short 400 High Biomolecule Binding DNA Binding 1.0µm 800 DNA Isolation 140 DNA/RNA Purification Long 300 PCR Clean up 400 Plasmid Minipreps Glass fibre Short 800 Recovering DNA from gels 1.2µm Long Reverse Transcriptase Sample Clean up M13 phage preps Thymidine Uptake Cell homogenates, crude Cell fragments/membranes Cytotoxic/Cell Proliferation Signal transduction Mammalian Cells Capture Ordering Information Part No. DESCRIPTION QUANTITY PRICE PV-360047 96 Well PP, 300µL Short drip UHMW Polyethylene, MPS 25µm 50 $1095.00 PV-360041 96 Well PP, 300µL Long drip UHMW Polyethylene, MPS 25µm 50 $ 990.00 PV-360035 96 Well PP, 400µL Short drip UHMW Polyethylene, MPS 25µm 25 $ 534.00 PV-360026 96 Well PP, 400µL Long drip UHMW Polyethylene, MPS 25µm 25 $ 534.00 PV-360029 96 Well PP, 800µL Short drip UHMW Polyethylene, MPS 25µm 25 $ 720.00 PV-360056 96 Well PP, 2mL Long drip UHMW Polyethylene, MPS 25µm 25 $ 757.00 Other filtration plates available upon request. Please contact your local sales representative. Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 441

Syringe and Membrane Filters Filter Device Selection Consider the number of samples, the sample volume, and the filtering mechanism you prefer to use. Syringe Filters Filtration is achieved by pushing the sample through the membrane with a syringe or other luer-connection device. Syringe filters allow you to control the rate of flow, which can be critical with delicate samples. Syringe filters also allow you to filter into nearly any tube, vial, or column that represents the next step in your analysis. Centrifuge Filter Tubes Filtration is achieved by pulling the sample through the membrane with centrifugal force. This allows you to simultaneously filter as many samples as your centrifuge can hold, and captures your filtrate in a clean microtube. Pre-Cut Membrane Multiple diameters offer a wide range of sample capacities, up to multiple liters. You need a filtration apparatus, such as a filter funnel or other vacuum-driven filtration device. Device Capacities Sample Volume Description Syringe Filter Capacities ≤1mL ≤30mL 4mm Syringe Filter ≤50mL 13mm Syringe Filter ≤100mL 17mm Syringe Filter ≤100mL 25mm Syringe Filter 30mm Syringe Filter 20µL to 500µL Centrifuge Filter Tube Capacities 1mL to 25mL 2mL Tubes 50mL Tubes up to 20mL Pre-Cut Membrane Capacities up to 100mL 13mm Pre-Cut Membranes multi-liter 25mm Pre-Cut Membranes 47mm Pre-Cut Membranes Membrane selection Choose a filter membrane based on the size and amount of particulate in the sample, the membrane’s chemical compatibility with the sample matrix, and potential interactions (binding) between the membrane and the sample components. This table offers general guidelines on membrane characteristics and applications. Membrane Selection Guide Solvent Protein Resistance Binding Membrane Type Features Common Uses Hydrophilic Good Medium Nylon Yes Good chemical compatibility and very General filtration High* High Polytetrafluoroethylene low extractables sterilization, HPLC sample prep No (PTFE) High Low Polyvinylidene Fluoride Compatible with strong acids and Gas, air, and solvent filtration (PVDF) aggressive solvents High Very Low** Regenerated Cellulose HPLC sample preparation and Yes Good flow rate characteristics. general filtration Yes *Highest solvent resistance. Ideal for chromatography applications. **Lowest protein binding. HPLC sample prep Universal membrane with excellent general filtration chemical resistance and low extractables. Use for aqueous and organic samples. 442 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

Syringe and Membrane Filters InnoSep™ Canadian Life Science Offers Affordable, Quality Syringe Filters Housing Membrane Polypropylene housings are highly solvent resistant and The cellulose acetate and polysulfone membranes are all low exhibit low extractables. They are ideal for filtering samples or protein binding, which makes them well suited for filtration of solvents used in chromatography. biological samples. PTFE membrane in a PP housing can be used to filter solvents Size and strong acids. Although PTFE is inherently hydrophobic, it can be made 4mm syringe filters are useful for filtration up to 1mL of low par- hydrophilic by pre-wetting the membrane with methanol and ticulate level chromatography of biological samples. then rinsing with water. 13mm syringe filters have a filtration area of 1.3cm2 and a hold- Nylon or polypropylene are ideal for filtration of chromatography up volume of less than 30uL with an air purge. samples. Ideal for samples up to 12mL. Polypropylene membranes exhibit near zero extractables, are 25mm syringe filters have a filtration area of 4.9cm2 and offer the very resistant to solvents and most chemicals and would be the fastest sample filtration. first choice for filtration of chromatography samples. Next to our excellent InnoSep™ line, we are distributor for the GE Osmonics transfer membranes. GE Osmonics Laboratory Products is the only manufacturer who actually produces all the membranes they sell into the tissue culture media preparation, LC sample preparation, transfer membrane and microbiological detection applications. GE Osmonics is a leading manufacturer and worldwide marketer of high-technology water purification, fluid filtration, fluid separation and fluid handling equipment and components. Looking for filter vials? See page 505. Material Description Features Application Pore Size CA Internally supported • Lowest binding material available • Tissue culture media sterilization 0.22 - 5.0 cellulose acetate • Highest throughput • Protein & enzyme filtration sterilization Glass Fiber • Strength and dimension stability • Biological fluid filtration, sterilization NC Glass fiber prefilters • Uniform pore structure • Tissue culture media sterilization • Hydrophilic Nylon Unsupported • Gravimetric analysis 0.5, 0.7, 1.0 PP nitrocellulose mixed • Biologically inert • Prefilter to extend final filter life PES cellulose esters • Available with or without acrylic binders • Clarification of particulate laden solutions • High dirt-holding capacity PVDF • Aqueous filtration 0.1 - 8.0 PTFE • Consistent flow rates • Microbiological analysis 0.1 - 20.0 • High throughputs • Sterility testing • Uniform pore structure • Gravimetric analysis with ashing technique • Hydrophilic • Particulate analysis Internally supported • Naturally hydrophilic • Sterilization, clarification of aqueous nylon • Wide chemical compatibility range and organic solvent solutions • Extremely low extractables Polypropylene • Strength and dimensional stability • LC sample preparation Polyethersulfone • Chemically and biologically inert • Sterilization, clarification of 0.1, 0.22, 0.45 • Wide chemical compatibility range organic solvent solutions 0.22 - 5.0 • Extremely low extractables • LC sample preparation • Low protein binding • Low extractables • Tissue culture media sterilization • Autoclavable • Protein and enzyme filtration, sterilization • Wide chemical compatibility range • Biological fluid filtration, sterilization • Strength and dimensional stability Polyvinylidene difluoride • Hydrophobic • Filtration of polar and non-polar solutions 0.2 - 0.45 • Chemically inert towards many solvents, similar stability to PTFE Polytetrafluoroethylene • Naturally hydrophilic • Filtration of strong acids 0.22, 0.45, 1.0 (Teflon laminated onto a • Compatible with strong acids and aggressive solvents polypropylene support) and aggressive solvents • Venting applications • Improved durability and handling See page 440 for Filter Membrane Compatibility Chart Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 443

InnoSep™ Syringe Filters InnoSep™ Syringe Filters • High quality filter with protective anti-burst ring • Available in 13mm and 25mm • Polypropylene housing for increased solvent resistance • Available in nylon, PTFE, polypropylene, polyethersulfone, cellulose acetate, nitrocellulose mixed esters, glass gibre and PVDF • Some filters are available with glass pre-filter Ordering Information Membrane Pore Size Part No. Price /100 25mm diameter SF5003-06 SF5112-06 $109.00 CA 0.2µm SF6055-06 $109.00 CA 0.45µm SF6054-06 $ 98.00 Nylon 0.2µm SF6059-06 $ 98.00 Nylon 0.45µm SF5509-06 $ 98.00 Nylon 5.0µm SF6550-06 $ 98.00 SF6057-06 $ 98.00 PES 0.45µm SF6058-06 $ 98.00 PP 0.2µm SF5510-06 $ 98.00 SF5511-06 $109.00 PTFE 0.2µm $109.00 PTFE 0.45µm SF6052-06 SF7500-06 $ 98.00 PVDF 0.2µm SF7504-06 $ 98.00 SF7501-06 $109.00 PVDF 0.45µm $109.00 SF6506-06 25mm filters with glass fiber membranes SF6507-06 $ 95.00 SF6502-06 $ 95.00 Glass Micro Fiber 0.7µm SF6503-06 $ 95.00 GF 1.0µm SF6500-06 $ 95.00 Nylon/GF 0.45µm SF6505-06 $ 95.00 0.45µm SF6504-06 $ 95.00 PTFE/GF $ 99.00 13mm diameter CA 0.22µm CA 0.45µm Nylon 0.2µm Nylon 0.45µm PTFE 0.2µm PTFE 0.45µm PVDF 0.45µm InnoSep™ Syringe Filters are available in a select number of filter media, diameters and pore sizes. Please contact us for ordering information on items not shown. 444 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

Syringe Filters - CHROMAFIL® Xtra CHROMAFIL® Xtra Disposable syringe filters CHROMAFIL® Xtra are ready-to-use filtration units, which are filter elements incorporated in a polypropylene housing. Because every filter is only used once, contaminations are avoided. Ordering Information Pore Size Part No. Price /100 Membrane 1.0µm MN729228 0.2µm MN729212 $109.00 25mm diameter 0.45µm MN729213 $109.00 GF 0.45µm MN729241 $109.00 PA (Nylon) 0.45µm MN729220 $109.00 PA (Nylon) 0.2µm MN729207 $109.00 PES 0.45µm MN729205 $109.00 PET 0.2µm MN729218 $109.00 PTFE 0.45µm MN729219 $125.00 PTFE 0.2µm MN729230 $125.00 PVDF 0.45µm MN729231 $109.00 PVDF $109.00 RC RC Disposable Syringe These disposable syringes are useful for sample preparation, especially when used with our syringe filters. Syringes are made from polypropylene and polyethylene and are silicone-free. Extractables are minimized compared with rubber-tipped plunger syringes. Syringes are individually wrapped in sterile “peel packs.” Ordering Information Part No. Description Quantity Price CR186603 3 mL Disposable Syringe 100 $ 41.40 CR186605 5 mL Disposable Syringe 100 $ 50.60 CR186610 10 mL Disposable Syringe 100 $ 57.50 CR186620 20 mL Disposable Syringe 100 $ 87.60 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 445

Microfiltration Spin Filters, Receiver Tubes Micro-Spin Centrifuge Filters Ordering Information - Midi-Spin - Sample volumes of 20µl to 850µl 4mL capacity with 7.0mL receiver tubes, non-sterile Part No. Membrane Price /25 Midi-Spin Centrifuge Filters CMBNY02 .2µm Nylon $ 57.00 CMBPE02 .2µm PES $ 57.00 - Sample volumes of 4mL to 7mL CMBPV02 .2µm PVDF $ 66.00 CMBRC02 .2µm RC $ 57.00 Maxi-Spin Centrifuge Filters CMBPT02 .2µm PTFE $ 66.00 CMBCA02 .2µm CA $ 57.00 - Sample volumes of up to 24mL CMBCA45 .45µm CA $ 57.00 CMBNY45 .45µm Nylon $ 57.00 Ordering Information - Micro-Spin CMBRC45 .45µm RC $ 57.00 CMBPT45 .45µm PTFE $ 66.00 800µL capacity with 2.0mL receiver tubes, non-sterile CMBPE45 .45µm PES $ 57.00 CMBPV45 .45µm PVDF $ 66.00 Ordering Information - Maxi-Spin 25mL capacity with 50mL receiver tubes, non-sterile Part No. Membrane Price /25 Part No. Membrane Price CMXNY02 .2µm Nylon $ 86.00 /100 CMXPE02 .2µm PES $ 86.00 CMCINY02 .2µm Nylon CMXPV02 .2µm PVDF $ 95.00 CMCIPE02 .2µm PES $ 80.00 CMXRC02 .2µm RC $ 86.00 CMCIPV02 .2µm PVDF $ 80.00 CMXPT02 .2µm PTFE $ 95.00 CMCIRC02 .2µm RC $ 91.00 CMXPP02 .2µm PP $ 86.00 CMCIPT02 .2µm PTFE $ 80.00 CMXCA02 .2µm CA $ 86.00 CMCIPP02 .2µm PP $ 91.00 CMXCA45 .45µm CA $ 86.00 CMCICA02 .2µm CA $ 80.00 CMXNY45 .45µm Nylon $ 86.00 CMCICA45 .45µm CA $ 80.00 CMXRC45 .45µm RC $ 86.00 CMCINY45 .45µm Nylon $ 80.00 CMXPT45 .45µm PTFE $ 95.00 CMCIRC45 .45µm RC $ 80.00 CMXPE45 .45µm PES $ 86.00 CMCIPT45 .45µm PTFE $ 80.00 CMXPV45 .45µm PVDF $ 95.00 CMCIPE45 .45µm PES $ 91.00 CMXPP45 .45µm PP $ 86.00 CMCIPV45 .45µm PVDF $ 80.00 CMCIPP45 .45µm PP $ 91.00 2mL, $ 80.00 No-Cap Receiver Tubes Additional plastic products offered include DNASE/RNase free receiver tubes in various sizes. Receiver tubes can be packaged with spin filters or separately. Ordering Information Part No. Membrane Quantity Price CM153-00 2.0mL with cap 5,000 $ 233.00 1.5mL 2mL, with cap CM152-BK 2.0mL no cap 5,000 $ 233.00 50mL CM158-00 1.5mL with cap 5,000 $ 233.00 CM176-00 0.5mL with cap 5,000 $ 233.00 Web: www.lifescience.ca CM184-00 50mL with cap 500 $ 148.00 446 Toll Free: 1-888-226-2775 E-mail: [email protected]

InnoSep™ Membrane Filters Membrane Filters Nylon Polyethersulfone (PES) Membrane • Naturally hydrophilic • Very low protein binding • Wide chemical compatibility range, • Fast flow rates, high throughput • Low extractables organic solvent resistance (except • Wide chemical compatibility range acids and bases) • Strength and dimensional stability • Internally supported • Autoclavable • Strength and dimensional stability • High binding capacity, high dirt loading capacity Nitrocellulose • Available in gridded and 1+ PK for testing Mixed Cellulose Ester (ME) Cellulose Acetate (CA) • Consistent flow rates • High throughput • Lowest binding material available • Uniform pore structure • Hydrophilic • Hydrophilic • High throughput • Strength and dimension stability Polyvinylidene Difluoride (PVDF) • Uniform pore structure • Hydrophobic Polytetrafluoroethylene (PTFE) • Suitable for filtration of polar • Naturally hydrophilic and non-polar solutions • Compatible with strong acids • Chemically inert towards many and aggressive solvents solvents, similar stability to PTFE • Improved durability and handling Ordering Information Pre Cut Size (mm) 13 25 47 90 Qty Per package 100 100 100 25 Nylon 0.2µm SFNY201300 $ 47.25 SFNY202500 $ 55.90 SFNY204700 $ 75.20 SFNY209025 $ 65.55 CA 0.45µm SFNY401300 $ 47.25 SFNY502500 $ 55.90 SFNY504700 $ 75.20 SFNY509025 $ 65.55 PTFE 0.2µm SFCA201300 $ 47.25 SFCA202500 $ 55.90 SFCA204700 $ 78.50 SFCA209025 $ 65.55 PES 0.45µm SFCA401300 $ 47.25 SFCA502500 $ 55.90 SFCA504700 $ 78.50 SFCA509025 $ 65.55 NC/ME 0.2µm SFPT201300 $ 47.25 SFPT202500 $ 55.90 SFPT204700 $ 81.50 SFPT209025 $ 65.55 PVDF 0.45µm SFPT401300 $ 47.25 SFPT502500 $ 55.90 SFPT504700 $ 81.50 SFPT509025 $ 65.55 0.2µm SFPS201300 $ 47.25 SFPS202500 $ 55.90 SFPS204700 $ 75.20 SFPS209025 $ 65.55 0.45µm SFPS401300 $ 47.25 SFPS502500 $ 55.90 SFPS504700 $ 75.20 SFPS509025 $ 65.55 0.2µm SFME201300 $ 47.25 SFME202500 $ 55.90 SFME204700 $ 81.50 SFME209025 $ 65.55 0.45µm SFME401300 $ 47.25 SFME502500 $ 55.90 SFME504700 $ 81.50 SFME509025 $ 65.55 0.2µm SFPV201300 $ 56.75 SFPV202500 $ 99.00 SFPV204700 $164.75 SFPV209025 $ 99.00 0.45µm SFPV401300 $ 56.75 SFPV502500 $ 99.00 SFPV504700 $164.75 SFPV509025 $ 99.00 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 447

Filtration Units InnoSep™ Glass Filter Holders For 47mm disc filters These convenient, high performance filter holders consist of a borosilicate glass funnel (300mL graduated) and base (with a fused-in coarse porosity fritted support; anodized aluminum clamp and a #8 silicone stopper to fit 1000mL filter flasks). Mating surfaces are precisely ground flat to assure a leak-resistant seal. The glass parts and the clamp are designed to work together to prevent any wrinkling or tearing of the filter membrane. Used fo analysis of bacteriological contamination or of suspended solids in water. Cleaning/preventing cross contamination: 1. Autoclave - 20 minutes at 121°C 2. Sterilize - 2-3 minutes in UV sterilizer 3. Sanitize - immerse briefly in sulfuric or hydrochloric acid Ordering Information Part No. Description Price Replacement parts available. Please contact us for more information. ISGFH25 25mm Filter Holder Kit $ 162.00 ISGFH47 47mm Filter Holder Kit $ 158.00 ISGFH90 90mm Filter Holder Kit $ 419.00 ISGF1000 90mm Filter Holder Kit $ 419.00 Kits consist of: 47mm 90mm 25mm • 300mL glass funnel with scale • 1000mL glass funnel with scale • 15mL glass funnel with scale • 25mm fritted glass base • 47mm fritted glass base • 90mm fritted glass base • #5 silicone stopper • 25mm aluminum clamp • #8 silicone stopper • #8 silicone stopper • 47mm aluminum clamp • 90mm aluminum clamp Analytical Funnel Autoclavable Filter Holders • Durable, break resistant, no extra parts to break or wear out • Uses a 47mm depth filter disc • 47mm filter funnel (<400 mL) allows for one-handed operation and has a unique gravity locking mechanism • No. 8 stopper mounts in a standard 1L filtering flask • No clamps, wheel locks or magnets to wear out • Only 2 parts • Solid, stable and easy to use Ordering Information Part No. Description Size Quantity Price Applications IS1213883 Base Only 47mm 1 $ 89.00 • Filtering liquids for sterility IS1213865 Complete Unit 47mm 1 $173.00 • Particle removal IS1214124 Complete Unit 47mm 3 $461.00 • General filtration IS1213882 Funnel Only 47mm 1 $ 89.00 448 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

Filter Paper With MACHEREY-NAGEL filtration products you can be sure that the grade for a particular application will always maintain its performance standards, consistency and quality. Qualitative Filter Papers MACHEREY-NAGEL qualitative filter papers are widely used for routine separations. The wide variety of grades provide a great choice of particle retention and filtration speed alternatives. Qualitative filter papers are manufactured from top quality raw materials using ultra pure water to ensure consistent and reliable filtration. MN 615 No. 1 Most widely used general purpose filter paper with medium retention and flowrate. This filter paper covers a wide range of laboratory applications and is fre- quently used for clarifying liquids. Filtration MN 616 md No. 2 Slightly more retentive than MN 615 with a corresponding increase in filtration Discover Please contact us time. for your copy Quality filters „Made in Germany“ since 1911 of the MN filtration MN 617 No. 4 Very fast filter paper with excellent retention of coarse particles and gelantinous Filter Circles catalogue precipitates. Used for example in routine clean up of biological or organic solutions. MN 619 Filter paper with very high particle retention and slow flow rate. Excellent clarifying Folded Filters Technical Cuts capacity for cloudy suspensions and for water and soil analysis. MN 619 Bulk Extraction Thimbles Membranes MACHEREY-NAGEL www.mn-net.com Ordering Information Filter circles and folded filters (packs of 100) MN 615 MN 616 md MN 617 Diameter (mm) $ 7.00 $ 28.00 $ 9.00 $ 32.00 $ 9l .00 $ 32.00 $ 7.00 $ 31.00 90 MN431009 MN531009 MN433009 MN533009 MN434009 MN534009 MN437009 MN537009 110 $ 9.00 $ 28.00 $ 13.00 $ 32.00 $ 12.00 $ 32.00 $ 9.00 $ 31.00 125 150 MN431011 MN531011 MN433011 MN533011 MN434011 MN534011 MN437011 MN537011 185 $ 10.00 $ 28.00 $ 15.00 $ 32.00 $ 15.00 $ 32.00 $ 11.00 $ 31.00 240 MN431012 MN531012 MN433012 MN533012 MN434012 MN534012 MN437012 MN537012 $ 13.00 $ 28.00 $ 19.00 $ 32.00 $ 19.00 $ 32.00 $ 15.00 $ 31.00 MN431015 MN531015 MN433015 MN533015 MN434015 MN534015 MN437015 MN537015 $ 18.00 $ 31.00 $ 26.00 $ 39.00 $ 26.00 $ 39.00 $ 20.00 $ 36.00 MN431018 MN531018 MN433018 MN533018 MN434018 MN534018 MN437018 MN537018 $ 30.00 $ 46.00 $ 43.00 $ 60.00 $ 43.00 $ 60.00 $ 32.00 $ 52.00 MN431024 MN531024 MN433024 MN533024 MN434024 MN534024 MN437024 MN537024 Quantitative (ashless) Filter Papers MACHEREY-NAGEL manufactures a broad range of ashless filter papers that are refined and purified in a highly developed clean-up process to remove organic and inorganic impurities. This ensures a low ash content of < 0.01%, consistent performance and safe results in gravimetric analysis. Ordering information Filter circles (packs of 100) MN 640 m MN 640 w MN 640 d No. 43 White label Classic, general purpose, ashless filter White ribbon paper with medium speed and medium retention. Typical applications include Diameter (mm) $ 22.00 $ 22.00 $ 22.00 MN 640 m gravimetric analysis for components, MN 640 w quantitative determinations of sediments 90 MN203009 MN202009 MN205009 MN 640 d and high purity clean up procedures. 110 $ 31.00 $ 31.00 $ 31.00 125 No. 41 Grey label Fast, ashless filter paper. 150 MN203011 MN202011 MN205011 Black ribbon Recommended for analytical procedures 185 $ 35.00 $ 35.00 $ 35.00 involving coarse particles or gelantinous 240 precipitates. MN203012 MN202012 MN205012 $ 50.00 $ 50.00 $ 50.00 No. 42 Green label Ashless filter paper with very fine Blue ribbon particle retention. Standard paper for MN203015 MN202015 MN205015 critical gravimetric analysis of fine $ 74.00 $ 74.00 $ 74.00 precipitates like barium sulfate or metastannic acid. MN203018 MN202018 MN205018 $121.00 $121.00 $121.00 MN203024 MN202024 MN205024 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 449

Filter Paper Glass Fibre Filters MACHEREY-NAGEL glass microfibre filters are manufactured from 100% borosilicate glass. They have wide applications when fine filtration and high loading capacity is required. Due to the intrinsic properties of borosilicate glass they also allow a safe filtration of aggressive solutions including strong acids and bases. Ordering Information Filter circles (packs of 25) Most widely used, general purpose glass MN 85/90 MN 85/90 BF MN GF-1 MN GF-3 fibre filter with very good mechanical stability. MN 85/90 The filter contains an organic binder and is stable up to 200°C. Diameter (mm) Binder free filter paper for difference weight 25 $ 16.00 $ 28.00 $ 28.00 $ 31.00 analysis. 37 MN 85/90 BF This paper is especially used in water 45 MN4050025 MN4060025 MN4110025 MN4130025 treatment facilities, for example, for the 55 $ 20.00 $ 36.00 $ 31.00 $ 36.00 determination of undissolved solids. 70 90 MN4050037 MN4060037 MN4110037 MN4130037 GF/A This binder free paper offers fine 110 $ 22.00 $ 40.00 $ 36.00 $ 45.00 retention, high flow rate and good 125 MN GF-1 loading capacity. It is used for high 150 MN4050045 MN4060045 MN4110045 MN4130045 efficiency laboratory filtration as well $ 24.00 $ 43.00 $ 39.00 $ 54.00 as air sampling. MN405005 MN406005 MN411005 MN413005 GF/C Slightly more retentive than MN GF-1 $ 31.00 $ 55.00 $ 51.00 $ 64.00 this binder free paper is used for the MN GF-3 collection of suspended solids in MN405007 MN406007 MN411007 MN413007 potable water as well as natural and $ 40.00 $ 73.00 $ 64.00 $ 92.00 industrial waste water in many parts of the world. MN405009 MN406009 MN411009 MN413009 $ 51.00 $ 93.00 $ 87.00 $129.00 MN405011 MN406011 MN411011 MN413011 $ 64.00 $117.00 $ 98.00 $165.00 MN405012 MN406012 MN411012 MN413012 $ 84.00 $155.00 $135.00 $233.00 MN405015 MN406015 MN411015 MN413015 Extraction Thimbles MACHEREY-NAGEL manufactures extraction thimbles from top quality raw materials using ultra pure water. They are typically used in Soxhlet extraction units, particularly for fat analysis of food and feed and for the analysis of soil contaminants. The thimbles allow the safe and efficient solvent extraction from solid materials. MN 645 Extraction thimble made of pure cellulose. MN 649 Glass microfibre thimbles for high temperature filtration or when using solvents incompatible with cellulose thimbles. Ordering Information - MN 645 (packs of 25) Ordering Information- MN 649 (packs of 25) Size IDxH (mm) MN 645 Size IDxH (mm) MN 645 Size IDxH (mm) MN 649 Size IDxH (mm) MN 649 20 x 80 $ 86.00 30 x 80 $ 86.00 22 x 80 $159.00 33 x 94 $190.00 22 x 80 30 x 100 33 x 80 MN649106 33 x 100 MN649122 28 x 80 MN645005 33 x 80 MN645020 29 x 100 $ 86.00 33 x 94 $103.00 $159.00 $190.00 MN649120 MN649123 MN645006 MN645023 $ 86.00 $ 93.00 MN645015 MN645951 $101.00 $103.00 MN645017 MN645022 Lab-Top, PE Coated Paper MN 210 PE Ordering Information - MN 210 PE This highly absorbent paper is coated on one side with polyethylene, providing an Part No. Description Price impermeable layer for the protection of the subjacent surface. Spilled liquids are MN112000 MN 210 PE 48x60 cm /100 sheets $211.00 effectively absorbed and can be removed cleanly. MN112020 MN 210 PE Reel 100 x 0.6 m $377.00 Weighing aids Ordering Information - MN 808, MN 226, MN 40/25 MN 808 These weighing boats are made from a special, nitrogen-free Part No. Description Price parchment. They are used to weigh viscous or syrupy substances. A very smooth transparent paper guaranteeing that the weighed MN486000 MN 808, 58x10x10mm /100 pcs $ 97.00 goods can be transfered without loss. MN 226 MN486001 MN 808, 70x23x15mm /100 pcs $135.00 Easy crushable parchment papers, especially used in the sugar MN186002 MN 226, 9x11.5cm, block of 100 sheets $ 12.00 industry. MN 40/25 MN194000 MN 40/25, 10x10 cm /1000 pcs $ 29.00 450 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

pH-Fix Test Strips pH-Fix Test Strips Established clear plastic Fast, accurate, reliable The classic pH-Fix box is made from a special high transparency plastic. This ensures optimal protection of the Always ready for use colour chart and a reliable readout. pH-Fix test strips are used for rapid pH testing directly at the R1 point of interest. They are always ready for immediate use. OC Coming with a complete colour chart they are readily calibrat- ed. As single use devices they are completely maintenance R2 free. For careful testing of dangerous, poisonous or aggresive Cellulose liquids the strips have the optimal design. The longest available handle effectively protects the user from contact with Dye the sample. This increases the safety of operation and adds handling ease. Patented technology Wide range of applications for optimal sample protection pH-Fix test sticks are appreciated by users worldwide. In the production the indicator reacts with the paper of the test They are the economic and easy standard for applications, pad to form a chemical bond. The dye is fixed to the test strip e.g.: and can not bleed into the sample. This protects the sample against contamination and enables measurements in weakly • Aquaculture buffered or strongly alkaline solutions. • Agriculture • Chemical laboratories Brilliant colours for exact results • Industry • Dairies pH-Fix test strips feature up to four different indicator pads to • Production cover the complete pH-range. The colour chart exactly • Physician offices matches both colour and position of the pads on the strips. • Hospitals This enables highly precise pH-determination and makes the • Schools test strips rapid and reliable. • Universities • Metal workshops Convenient flat boxes • Fire brigades • Police Users appreciate the quality of pH-Fix. Its classic design • Authorities requires small space and fits into the breast pocket of every • Food processing industry lab coat. • Surface treatment and many more Wide variety of pH-Fix Test Strips available for all applications listed above. Please contact us for more information! pH Test 0-14 Ordering Information Contents: 100 Strips Catalogue # MN92110.CLS 7 Part No. Description Quantity Price 6 100 $ 23.75 5 4 3 2 1 0 MN92110.CLS Test strips, 6 x 85mm, pH-Fix 0-14 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 451

FunnelFit Filters XENOSEP FunnelFit™ Filters Drop & Go! for extract drying applications Pre-formed conical filter • Saves time and solvent • Reduces cost and increases lab productivity For about the same price as your current filter, new FunnelFit™ conical filters eliminate the mundane and frustrating preparation steps required when working with cumbersome, ill-fitting, pre-pleated or quadrant folded filters. The pre-formed conical filter fits the funnel perfectly every time which improves quality by preventing sample bypass and tip blow-out. Using FunnelFit™ conical filters is 10 times faster than quadrant folding circular filters which improves laboratory productivity. Eliminate folding, repositioning, and pre-wetting hassles to save time and solvent costs FunnelFit™ Conical Filters fit the funnel perfectly ensuring high quality results Ordering Information Part No. Description Quantity Cross Reference Price to Whatman IS2440-125 125mm DA-Medium (8-12 µm) 100 $ 58.00 IS2440-150 150mm DA-Medium (8-12 µm) 100 1440-125 $ 77.00 IS2441-125 125mm DA-Coarse (20-25 µm) 100 1440-150 $ 58.00 IS2441-150 150mm DA-Coarse (20-25 µm) 100 1441-125 $ 77.00 IS2442-125 125mm DA-Fine (2-3 µm) 100 1441-150 $ 58.00 IS2442-150 150mm DA-Fine (2-3 µm) 100 1442-125 $ 77.00 1442-150 Other diameters and grades available upon request. 452 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

QuEChERS Canadian Life Science now offers QuEChERS (Quick Easy, These products make it simple to prepare your food samples Cheap, Effective, Rugged and Safe… pronounced \"katchers\") for analysis with: products for determining pesticide levels in food samples. • High recoveries Basic Method for using QuEChERS • Accurate results • High sample throughput • Minimal solvent use • Less labour • Lower costs • Simple glassware Sample Add Acetonitrile Original Method Buffered 10g samples AOAC 2007.1 Method 15g samples Original Method Buffered 15g samples EN 15662 Method Aliquot: 10g samples 1mL, 6mL or 8mL Check pH and adjust to 5 - 5.5 Add internal standard shake and centrifuge Aliquot: 1mL, 6mL or 8mL General Fruits & General Fruits & Vegetables Vegetables 2mL & 15mL kits 2mL & 15mL kits Pigmented Fruits Pigmented Fruits & Vegetables & Vegetables 2mL & 15mL kits 2mL & 15mL kits Fatty/Waxy Fruits Fatty/Waxy Fruits & Vegetables & Vegetables 2mL & 15mL kits 2mL & 15mL kits Fruits & Vegetables Fruits & Vegetables with Fats, Pigments with Fats, Pigments 2mL & 15mL kits 2mL & 15mL kits AOAC Method EN Method Analysis Shake and Centrifuge Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 453

QuEChERS Ordering Information Part No. Description Volume Recommended Application Qty. Price /100 QuEChERS Kit with Instructions $215.00 XE-29500 AOAC 2007.1 50/2 AOAC 2007.1 50 $105.00 50mL Centrifuge Tubes for Sample Extraction 50 $ 94.00 XE-29501 4g MgSO4, 1g NaCl, 50 Mini-Multiresidue, European prEN-15662 50 XE-29502 1g trisodium citrate dihydrate, $127.00 XE-29503 .5g disodium hydrogencitrate 50 50 XE-29504 sesquihydrate $105.00 50 50 $116.00 4g MgSO4, 1g NaCl 50 $ 55.00 50 AOAC 2007.1 50 6g MgSO4, 1.5g NaCl, 1.5g 50 $138.00 sodium citrate dibasic 2 Mini-Multiresidue, European prEN-15662, $143.00 750mg disodium citrate 2 AOAC 2007.1 50 $143.00 dibasic sesquihydrate 2 $154.00 2 $143.00 6MgSO4, 1.5g NaOAc 2 $154.00 2 $204.00 XE-29505 6g MgSO4, 1.5g NaOAc, 2 $215.00 750mg disodium citrate sesquihydrate 2 $116.00 XE-29506 Empty 50mL Centrifuge Tube 15 $127.00 15 $292.00 2mL Micro-Centrifuge Tubes 15 $105.00 15 $116.00 XE-29507 150mg MgSO4, 25mg PSA 15 Mini-Multiresidue, European prEN-15662 100 XE-29508 150mg MgSO4, 25mg PSA, 25mg C18 Mini-Multiresidue 100 $149.00 XE-29509 150mg MgSO4, 25mg PSA, 2.5mg GCB 15 Mini-Multiresidue, European prEN-15662 100 $116.00 XE-29510 150mg MgSO4, 25mg PSA, 7.5mg GCB 15 Mini-Multiresidue, European prEN-15662 100 $204.00 XE-29511 150mg MgSO4, 50mg PSA 15 AOAC 2007.1 100 $138.00 XE-29512 150mg MgSO4, 50mg PSA, 50mg C18 15 AOAC 2007.1 100 XE-29513 150mg MgSO4, 50mg PSA, 50mg GCB AOAC 2007.1 100 $182.00 6 $204.00 XE-29514 150mg MgSO4, 50mg PSA, 50mg C18, 6 AOAC 2007.1 100 $286.00 50mg GCB 6 15mL Micro-Centrifuge Tubes XE-29515 1200mg MgSO4, 400mg PSA AOAC 2007.1 50 XE-29516 AOAC 2007.1 50 1200mg MgSO4, 400mg PSA, AOAC 2007.1 50 XE-29517 400mg C18 European prEN-15662 50 XE-29518 European prEN-15662 50 XE-29519 1200mg MgSO4, 400mg PSA, European prEN-15662 50 400mg C18, 400mg GCB European prEN-15662 50 NA 50 900mg MgSO4, 150mg PSA - 50 900mg MgSO4, 150mg PSA, 15mg GCB XE-29520 900mg MgSO4, 150mg PSA, XE-29521 45mg GCB 900mg MgSO4, 150mg PSA, 150mg C18 900mg MgSO4, 300mg PSA, XE-29522 150mg GCB XE-29523 900mg MgSO4, 300mg PSA, 150mg C18 6mL SPE Column with PTFE Frits XE-29524 200mg GCB/400mg PSA Used in Schenck variation 30 XE-29525 250mg GCB/500mg PSA Used in Schenck variation 30 XE-29526 500mg GCB/500mg PSA Used in Schenck variation 30 454 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

TCLP XENOSEP TCLP Filters Xenosep offers two types of TCLP filters suitable for use in EPA Method 1311 depending on the analytes of interest. Both types of TCLP filters are binder-free, borosilicate glass microfiber filters with an effective pore size of 0.7 +/- .01µm as specified in the method. Volatiles Metals or non-volatiles Use Xenosep Standard-Certified TCLP Filters. These filters Use Xenosep acid-washed TCLP filters. These filters are are tested for several important parameters and are certified pre-washed in 1N HNO3 acid and then rinsed with deionized to meet the specification above. water according to EPA Method 1311 to save you time, reduce waste and provide low background blank values. A certificate of analysis demonstrating background TCLP metal values is also included in each box. Ordering Information Ordering Information Part No. Diameter (mm) Price Part No. Diameter (mm) Price /100 /100 Standard-Certified TCLP Filters for Volatiles Acid-Washed TCLP Filters for Metals $ 87.00 and Non-Volatiles $109.00 XE-306047 47 $ 43.00 $120.00 $119.00 XE-306076 76 $ 54.00 XE-307047 47 $165.00 $296.00 XE-306090 90 $ 65.00 XE-307076 76 $362.00 XE-306110 110 $120.00 XE-307090 90 XE-306125 125 $153.00 XE-307110 110 XE-306142 142 $175.00 XE-307125 125 XE-306150 150 $219.00 XE-307142 142 XE-307150 150 EPA Method 1311 (TCLP), Section 4.4: “When evaluating the mobility of metals, filters shall be acid washed prior to use with 1N nitric acid followed by three consecutive rinses with deionized distilled water (a minimum of 1 L per rinse is recommended).” Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 455

TSS Filters for Standard Methods 2540 C and 2540 D Ensure worry free audits by taking the guesswork out of method compliance Xenosep TSS Filters are specifically designed to ensure compliance with the filter preparation requirements of Standard Methods 2540 C (Total Dissolved Solids Dried at 180°C) and Standard Methods 2540 D (Total Suspended Solids at 103-105°C). We removed the guesswork typically associated with audit checklist question #6 by making the wrinkle side of the filter visibly different than the screen side. Now everyone can clearly see and easily determine which side needs to face up to ensure compliance. Wrinkle side faces up In addition to more distinguishable filter surface characteristics Screen side faces down shown at left, these 1.5 µm binder-free, fast flowing, fine porosity borosilicate glass microfiber filters have been opti- mized to eliminate pin holing and minimize fiber shedding; two quality issues known to affect other brands of TSS filters. Xenosep TSS Filters are readily available in the popular sizes below for use in a wide variety of CoorsTek chemical-porcelain labware including Bitumen, Buchner, Gooch, Hirsch, and many other popular filter holders. Other sizes are available upon request. Ordering Information Part No. Diameter (mm) Fits CoorsTek# Price /100 XE-301020 20 Gooch - 60148 $ 20.00 XE-301021 21 Gooch - 60148 $ 20.00 XE-301024 24 Gooch - 60151 $ 20.00 XE-301025 25 Hirsch - 60299 $ 20.00 XE-301030 30 Gooch - 60153 $ 25.00 XE-301032 32 Bitumen - 60170 $ 25.00 XE-301035 35 Buchner - 60239 $ 25.00 XE-301037 37 Buchner - 60239 $ 25.00 XE-301042 42.5 Hirsch - 60301 $ 27.00 XE-301047 47 Hirsch - 60301 $ 29.00 XE-301055 55 Buchner - 60240 $ 36.00 XE-301070 70 Buchner - 60242 $ 43.00 XE-301090 90 Buchner - 60243 $ 57.00 XE-301110 110 Buchner - 60244 $ 75.00 XE-301125 125 Buchner - 60245 $ 98.00 XE-301150 150 Buchner - 60246 $127.00 456 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

SPE Systems for EPA Method 1664A XENOSEP® SPE Systems for EPA Method 1664A Step 1 • Extract samples 5 times faster, • Distill samples 3 times faster • Significantly lower cost per test The first SPE filter and glass apparatus specially designed for dirty wastewater samples. Integral design maximizes filtration efficiency, simplifies analyte recovery, and ensures reproducible results. Step 1 Step 3 Step 2 Extract 1L sample Note: Lab stand and 17 seconds is all it takes to filter a 40 mg/L* OPR and achieve clamps not included 98% recovery. Thatʼs 50 times faster than a typical single layer SPE filter with 89% recovery. In addition, triple layer Xenosep Table 1 SPE Filters have 5 times the filtration capacity already built-in for the MS, MSD, and hard-to-filter samples. Compare the Options *As required by EPA Method 1664A Process Step Xenosep® SPE Typical SPE Hexane LLE Step 2 Extract-Elute-Dry 8.8 min. 26.5 min. 46.0 min. Elute and dry extract Less filter clogging enables more efficient filter drying and Distill-Oven Dry 21.0 min. 50.0 min. 60.0 min. eluting with less solvent. Xenosep sodium sulfate columns Time to Dessicator 29.8 min. 76.5 min. 106.0 min. save time by eliminating off-line drying steps or repouring the extracted water sample back into the separatory funnel. Estimated Labor/Batch of 20 4 hrs. 6 hrs. 8 hrs. 2 hrs. 4 hrs. Xenosep Labor Savings/Batch Step 3 Elution/cleaning apparatus Distill and oven dry extract • Unique integral apparatus increases productivity Less extraction solvent means faster distillation. Final hexane and lowers cost per result extracts distill to dryness in about 10 minutes. Unique flat sided Xenosep flasks reduce oven drying times by 67% so you • Disposable prepacked sodium sulfate cartridge get QC tests in the dessicator 3 times faster (see Table 1). maximizes recoveries, speed, and eliminates off-line transfers Conditioning/filtration apparatus • Dedicated station for sample elution, • Short wide funnel for easy access drying and CIP (clean-in-place) glassware and effective glassware rinsing solvent rinsing • Built in space for optional prefilters, • Universal ST 24/25 joint for collection into filter aids, and/or drying agent additions standard ST 24/40 flask to “wet” solids • Flat sided flask gently evaporates extract • Secure coupler maximizes effective to increase recoveries and lower MDLs filtration area and prevents assembly misalignment Distillation/Evaporation Apparatus • Teflon ® encapsulated O-ring seal • Compact cross-over condenser design simplifies eliminates solvent and vacuum leaks solvent recovery and provides analyst protection without extractables • Flat sided flask optimized to reduce • Solid rimmed stainless steel support distillation/evaporation times prevents analyte bypass • N2 inlet provided for safety and faster • Flared collection tube for easy removal distillation/evaporation • Wider internal diameter stem maximizes filtration rates • Universal ST 40/35 joint connects to elution apparatus or standard multi-place manifold, optimized to accept 30mm OD vials or collection tubes Easy to clean glassware minimizes breakage and fits standard washers Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 457

SPE Systems for EPA Method 1664A Xenosep® SPE Fast Throughput High Capacity Filters Unique triple layer construction offers fastest sample Filtration Extraction Elution throughput and highest filtration capacity • No emulsions or Freon® non-compliance 5-25 µm Prefilter • Assure legally defensible data • Achieve higher quality results 1-5 µm Prefilter • Reliable and consistent performance Adsorbent Layer • Decrease sample turnaround time • Eliminate additional prefilter expense • Significantly reduce operating cost Filter dirty samples 10 times faster Typical SPE Filters 1 L in Cutaway views of how the triple layer Xenosep SPE Filter 6.5 Min. (Recovery 79.9%) 1200 Xenosep ® SPE Filters 1 L in 38 Sec. (Recovery 93.4%) 1000 800 Sample Filtered, (mL) separates analytes from particulate and unwanted soluble interferences PAR Standard Filtered (mg)600Green–3 µm Particulates PAR Standard Filtered (L)•• Red–Oil and Grease Analytes Sample: Blue–Soluble Interference • Black–8 µm Particulates 400 40 mg/L* EPA 1664A Std. •• Brown–30 µm Particulates Plus 14 g of Solids Added 200 0 Extra thick fluffy layer 0 1 2 3 4 5 6 7 8 9 10 suspends solids filter cake and prevents Time (Minutes) premature clogging of dirty samples Temperature: 21°C, Vacuum: 15˝ Hg SPE Filters–47 mm Diameter Why are 40 mg/L PAR comparisons important? EPA Method 1664A requires the PAR concentration as follows: SPE speed and filtration capacity 40 mg/L* “The weight must be 40 ± 1 mg” (Section 7.10.3 NOTE) and at EPA 1664A PAR standard least this amount are required for the QCS, OPR, MS, MSD, and IPR samples. (Sections 9.8, 9.7, 9.6.1, 9.3.1, 9.2.2, 7.11 240 6 and 7.10) Although certain method modifications are allowed, 200 a change in the PAR concentration is not expressly permitted 160 Xenosep SPE Filters 1 L in Xenosep SPE Filters 5.5 L 5 and “shall be considered a major modification subject to appli- 120 17 Sec. (Recovery 97.6%) in 20 Min. (Recovery 95.5%) 4 cation and approval of alternate test procedures under CFR 80 3 136.4 and 136.5.” (Sections 1.7 and 1.8) 40 Xenosep SPE Filters Have FIVE 2 Times Greater Filtration Capacity Why expose the defensibility of your data to unnecessary legal 0 risk? Use 40 mg/L QC samples and achieve higher quality Typical SPE Filters 1 L in 19.2 minutes (Recovery 89.3%) 1 results with Xenosep SPE Filters. 0 2 4 6 8 10 12 14 16 18 20 Time (Minutes) Temperature: 21°C, Vacuum: 15˝ Hg SPE Filters–47 mm Diameter *As required by EPA Method 1664A Ordering Information Part No. Description Price XE-24547 SPE Filters, 1664A, 47mm (48pk) Call 458 Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca

SPE Systems for EPA Method 1664A Xenosep SPE Systems for performing EPA Method 1664A are Xenosep Manifold Kits are available for a three position available in convenient Starter Kits for laboratories just manifold and include components necessary to process three starting out. Starter Kits include all components required to samples at a time. process one sample at a time. Additional components are available for scale up as required. Note: Manifold sold separately Ordering Information XENOSEP® SPE starter kit Part No. Description Price Call XE-26616 Starter Kit, 47mm, 1 Place Qty Part No. for Kit Components Included* 1 each XE-26603; XE-26604; XE-26610; XE-26611; 2 each XE-26613; XE-26614; XE-26615; XE-26508; 3 each XE- 26514; XE-26515; XE-26516; XE-26517 7 each XE-26608 Ordering Information XE-26512 XENOSEP® SPE manifold kit XE-26513 Part No. Description Price Call * See Replacement Parts for description XE-26618 Manifold Kit, 47mm, 3 Place Call Call Part No. for Kit Components Included* Call Ordering Information XE-26615; XE-26512; XE-26516; XE-26519 Call Additional apparatus assembly kits XE-26603; XE-26605; XE-26610; XE-26611; XE-26507; XE-26613 Part No. Description Qty Price XE-26608 XE-26511 XE-26526 Distillation Kit, Complete Call XE-26600 XE-26513; XE-26514; XE-26515 1 each * See Replacement Parts for description XE-26601 XE-26512 3 each Ordering Information Flask Kit, Flat Sided, TS 24/25, 125 mL Call Replacement parts and components XE-26513 12 each SPE Filtration Assembly, 47mm, Starter Call Part No. Description Qty Price XE-26603; XE-26604; XE-26610; XE-26507 Vials, Collection, 30 x 125mm 1 Call XE-26611; XE-26613 1 each XE-26508 Tube, Collection, 30 x 200mm 1 Call XE-26608 2 each XE-26512 Clamp, Polyoxymethylene Acetal, TS 24/25 1 Call SPE Filtration Assembly, 47mm, Manifold Call XE-26513 Flask, Flat Sided Erlenmeyer, TS 24/25, 125mL 1 Call XE-26603; XE-26605; XE-26610; 1 each XE-26514 Flask, Round Flat Bottom, TS 24/25, 125mL 1 Call XE-26611; XE-26613 XE-26515 Condenser, Crossover w/#2HB 1 Call XE-26608 2 each XE-26516 Eluter, Glass, w/#2HB 1 Call Consumables Kit, 24 Tests 24 each XE-26620 XE-24547; XE-26520; XE-26524 $172 XE-26517 Stopcock, Glass, 120° 3 way, 4mmID 1 Call XE-26519 Stopcock Assembly, Manifold 1 Call XE-26523 Vent Assembly, Manifold 1 Call Ordering Information XE-26603 Funnel, 300 mL, 47mm 1 Call Consumables XE-26604 Holder, Starter, 47mm 1 Call XE-26605 Holder, Manifold, 47mm 1 Call Part No. Description Qty Price XE-26608 O-Rings, FEP-Viton®, Coupler, 47mm 1 Call XE-24547 SPE Filters, 1664A, 47mm 48 Call XE-26610 Coupler, PTFE, 47mm 1 Call XE-26520 Pipets, Transfer, Polyethylene, 15mL 48 $ 14.00 XE-26611 Support, Stainless Steel, 47mm 1 Call XE-26524 Column, Inline, Sodium Sulfate, 10g 48 $126.00 XE-26613 Clamp, Aluminum, 47mm 1 Call XE-26528 Fibers, Prefilter, 50g 1 $ 83.00 XE-26614 Flask, TS 40/35, 1L 1 Call XE-26532 EPA 1664A Std., 1 x 100 mL, 4 mg/mL 1 $ 11.00 XE-26615 Tubing, Tygon®, 3/8˝ ID x 5/8˝ OD, 3´ 1 Call XE-26533 EPA 1664A Std., 12 x 10 mL, 4 mg/mL 12 $ 5.00 XE-26619 Manifold, Complete, Stainless Steel, 3 Place 1 Call Toll Free: 1-888-226-2775 E-mail: [email protected] Web: www.lifescience.ca 459