AFFINIMIP® SPE Chloramphenicol DETERMINATION OF CHLORAMPHENICOL IN SHRIMP Regulations for Chloramphenicol in Intensity RESULTS residues in food of animal origin: Europe (2003/181/EC) : prohibited with a 65000 Chloramphenicol Minimum Required Performance Limits of 0.3µg/Kg 60000 USA FDA: prohibited 55000 50000 PROTOCOL OF CLEANUP 45000 Sample preparation 40000 5g peeled shrimp were homogenized 2min 35000 with a vortex in 20mL of ethyl acetate. Then 30000 the solution was filtered on filter paper 25000 (25µm). The supernatant was evaporated to 20000 dryness and reconstituted in 10mL of Water to 15000 obtain the loading solution. 10000 Cleanup with a 1mL/50mg AFFINIMIP® SPE 5000 Chloramphenicol cartridge 0 0 2 4 6 8 10 12 14 16 Equilibration Time (min) •2mL Acetonitrile •2mL Water SIM Chromatograms obtained after clean-up with AFFINIMIP® SPE Chloramphenicol of Shrimp spiked Loading with Chloramphenicol at 38µg/kg. Loading of 1mL 1 or 2mL of loading solution (spiked in green and not spiked in black) and of 2mL (spiked in red and not spiked in blue) Washing of interferents (W1) •1mL Water UV: VERY LOW BACKGROUND •1mL (Water - 0.5% Acetic Acid)/Acetonitrile (95/5) 9000 •2mL of Ammonia (1%) in Water •2mL (Water-1% Ammonia)/Acetonitrile 8000 Chloramphenicol (80/20)) 7000 Drying 1 min Washing of interferents (W2) 6000 0.25mL Diethyl ether 5000 Elution (E) uAU 4000 2mL Methanol The elution fraction was then evaporated and 3000 dissolved in the mobile phase before HPLC analysis. 2000 HPLC Method with MS detection 1000 Column: Thermo Accucore C18 column 50mm x 2.1mm 0 Mobile phase: Ammonium acetate (10mM) in water /Methanol (75/25) -1000 flow rate: 0.2mL/min MS detection: m/z 321 (ESI-) 1 2 3 4 5 6 7 8 9 10 11 12 13 Injection volume: 20µL. Time (min) UV Chromatograms obtained after clean-up with AFFINIMIP® SPE Chloramphenicol of Shrimp spiked with Chloramphenicol at 38µg/kg. Loading of 1mL (spiked in green and not spiked in black) and of 2mL (spiked in red and not spiked in blue) Recovery of Chloramphenicol spiked at 38µg/kg after AFFINIMIP® SPE Chloramphenicol clean-up of Shrimp. C° (µg/kg) Loading Mean Recovery volume (µg/kg) % 38 1mL 38.7 101.7 38 2mL 36.4 95.8 Catalog number: 1mL-50mg sorbent PI-FS110-02A for 25 cartridges PI-FS110-03A for 50 cartridges 51
DG102 AFFINIMIP® SPE Amphetamines Amphetamines are a class of illegal drugs that exhibit strong central nervous system stimulant effect. So, to determine if drivers are under influence of (Meth)amphetamine, several US and European states have set up a cut-off value in urine or blood [e.g. France and Virginia (respectively 50ng/mL and 100ng/mL of blood)]. For such low concentrations, a clean up step is crucial in order to improve the sensitivity, the reliability and the specificity before LC analysis. H NH2 N Amphetamine Methamphetamine H N O NH2 O O O 3,4-Methylenedioxyamphetamine 3,4-Methylenedioxymethamphetamine MDA MDMA O H N O 3,4-methylenedioxy-N-ethylamphetamine MDEA To do so, we have developed a AFFINIMIP® SPE Amphetamines cartridge, a powerful technique for clean-up and pre-concentration applications of Amphetamines. These application notes describe the solid phase extraction of Amphetamines from human urine and human serum using AFFINIMIP® SPE Amphetamines. Catalog number: 3mL-50mg cartridge PI-DG102-02 for 25 cartridges PI-DG102-03 for 50 cartridges 52
AFFINIMIP® SPE Amphetamines DETERMINATION OF AMPHETAMINES IN HUMAN URINE Example of Regulations: RESULTS France : prohibited cut-off limit of 1µg/mL in urine and 50ng/mL of blood 85 Virginia (USA): 100ng/mL of blood 80 Amphetamine 75 70 65 60 MDA 55 50 45 40 Methamphetamine 35 PROTOCOL OF CLEANUP 30 Sample preparation Human urine is diluted by 2 with an ammonium 25 acetate buffer (13mM, pH 8.5). The pH of the diluted urine is adjusted with NH3 or CH3COOH 20 MDMA at pH 8.5. 15 10 MDEA 5 0 2 4 6 8 10 12 14 16 18 20 22 24 Time (min) Mass Chromatogram (SIM) obtained after AFFINIMIP® SPE Amphetamines clean-up of a Cleanup with a 3mL AFFINIMIP® SPE human urine sample spiked at 20ng/mL with Amphetamines cartridge Amphetamine and its derivatives. Equilibration Recovery of Amphetamines in human urine spiked •1mL Acetonitrile at 20ng/mL after AFFINIMIP® SPE Amphetamines •2mL Water clean-up and relative standard deviation calculated from results generated under reproducibility Loading conditions. 5mL of diluted urine Sample Mean Recoveries % RSDR Washing of interferents (W1) •3mL Water ng/mL % 8.9 (n=8) •3mL Water/Acetonitrile (60/40) 9.6 (n=8) Amphetamine 17.5 87.5 9.2 (n=8) Drying 30 seconds MDA 18.6 93.1 1.5 (n=4) Elution (E) 18.6 93.2 12.4 (n=8) Methamphetamine 21.1 105.4 1.5mL Methanol – 2% Formic acid MDMA 20.3 101.7 The elution fraction was then evaporated and MDEA dissolved in the mobile phase before HPLC analysis. HPLC Method with MS detection Capacity: different concentrations of Amphetamine Column: Syncronis Aq column 150mm x 2.1mm in urine were applied on AFFINIMIP® SPE Mobile phase: gradient profile with A (Water – Amphetamines cartridge (25mg) to measure the Ammonium Acetate 10mM) and B (Acetonitrile – capacity of the product. Ammonium Acetate 1mM) Quantity loaded Quantity obtained in the elution Time (min) %A %B µg fraction 0 95 5 µg 2 95 5 1.0 0.90 12 60 40 2.5 2.41 95 5 5.0 3.51 12.1 flow rate: 0.4mL/min Catalog number: MS detection (ESI+) : m/z 136 (Amphetamine) ; 180 3mL-50mg cartridge (MDA); 150 (Methamphetamine); 194 (MDMA); 208 PI-DG102-02 for 25 cartridges (MDEA) PI-DG102-03 for 50 cartridges Injection volume: 20µL. 53
AFFINIMIP® SPE Amphetamines DETERMINATION OF AMPHETAMINES IN HUMAN SERUM Example of Regulations: RESULTS France : prohibited cut-off limit of 1µg/mL in urine and 50ng/mL of blood 90 Virginia (USA): 100ng/mL of blood 80 PROTOCOL OF CLEANUP Sample preparation Amphetamine Human serum is diluted by 5 with an ammonium acetate buffer (13mM, pH 8.5). The 70 pH of the diluted urine is adjusted with NH3 or CH3COOH at pH 8.5. 60 Cleanup with a 3mL AFFINIMIP® SPE MDA Amphetamines cartridge 50 Equilibration •1mL Acetonitrile 40 Methamphetamine •2mL Water 30 Loading 2.5mL of diluted serum 20 MDMA Washing of interferents (W1) 10 MDEA •3mL Water •3mL Water/Acetonitrile (60/40) 0 2 4 6 8 10 12 14 16 18 20 22 24 Drying 30 seconds Time (min) Elution (E) Mass Chromatogram (SIM) obtained after 1.5mL Methanol – 2% Formic acid AFFINIMIP® SPE Amphetamines clean-up of a The elution fraction was then evaporated and human serum sample spiked at 100ng/mL with dissolved in the mobile phase before HPLC Amphetamine and its derivatives. analysis. Recovery of Amphetamines in human serum spiked at 100ng/mL after AFFINIMIP® SPE Amphetamines clean-up and relative standard deviation calculated from results generated under reproducibility conditions (n=4). Sample Mean Recoveries % RSDR ng/mL % Amphetamine MDA 87.9 87.9 5.0 94.4 94.4 3.7 Methamphetamine 90.7 90.7 2.2 MDMA 106.2 106.2 2.5 MDEA 111.0 111.0 4.9 HPLC Method with MS detection Column: Syncronis Aq column 150mm x 2.1mm Mobile phase: gradient profile with A (Water – Ammonium Acetate 10mM) and B (Acetonitrile – Ammonium Acetate 1mM) Time (min) %A %B 0 95 5 2 95 5 12 60 40 95 5 12.1 flow rate: 0.4mL/min Catalog number: MS detection (ESI+) : m/z 136 (Amphetamine) ; 180 3mL-50mg cartridge (MDA); 150 (Methamphetamine); 194 (MDMA); 208 PI-DG102-02 for 25 cartridges (MDEA) PI-DG102-03 for 50 cartridges Injection volume: 20µL. 54
FS112 AFFINIMIP® SPE Tetracyclines Tetracyclines (TCs) like oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) are broad-spectrum antibiotics and are widely used as veterinary medicines and feed additives. These residues can cause toxic or allergic reactions in hypersensitive individuals and also transfer drug-resistant bacteria from food to humans. In response to these concerns and to prevent harmful effects of residual antibiotics in milk on the human health, various international health organizations have established the maximum residual limit (MRL) of TCs in all circulating milk in their countries. Worldwide maximum residue levels (MRL) for tetracycline antibiotics are 100ppb (µg/L). For such low concentrations, a clean up step is crucial in order to improve the sensitivity, the reliability and the specificity before LC analysis. Tetracycline Oxytetracycline Chlortetracycline To do so, we have developed a AFFINIMIP® SPE Tetracyclines cartridge, a powerful technique for clean-up and pre-concentration applications of Tetracyclines. Catalog number: 1mL-10mg sorbent PI-FS112-02A for 25 cartridges 1mL PI-FS112-03A for 50 cartridges 1mL kit of 12 reservoirs 15ml and adapters for use with 1, 3 & 6 mL columns PI-ACC-AR2 55
AFFINIMIP® SPE Tetracyclines DETERMINATION OF TETRACYCLINES, THEIR EPIMERS AND DOXYCYCLINE IN MILK AND SALMON PROTOCOL OF CLEANUP RESULTS Sample preparation for Milk Mix 1.5mL of Milk with 6mL of EDTA/Mc Ilvaine’s 80 80 Buffer and centrifuge at 4000rpm for 10 min at a temperature below 15°C. Collect the supernatant 70 CTC 70 and add 750µL 1N NaOH solution. Adjust to pH 10 60 with a NaOH solution (this mixture was the loading 4-epiOTC TC solution). Sample Preparation for Salmon based on AOAC 60 995.09 method Blend 10g Salmon with 40mL of EDTA/Mc Ilvaine’s 50 50 Buffer during 30 s and stir during 10min with a magnetic stirrer. Centrifuge the mixture at 2500g 40 40 for 10 min at a temperature < 15°C. Collect the supernatant mAU Repeat this operation with 40mL buffer and again mAU with 20mL of buffer. Then, gather all the supernatants and centrifuge during 20min at 2500g, 30 30 filter on Buchner. Add 750µL 1N NaOH solution to the filtrate and adjust to pH 10 with a NaOH 20 20 solution (this mixture was the loading solution). Cleanup with a 1mL/10mg AFFINIMIP® SPE 10 10 Tetracyclines cartridge 00 Equilibration •1mL Acetonitrile -10 -10 •1mL Water 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46 48 50 Loading Minutes Loading solution (7.5mL) UV Chromatograms (355nm) obtained after clean- Washing of interferents up with AFFINIMIP® SPE Tetracyclines of 1.5mL of •1mL Water Milk spiked with Tetracycline, Chlortetracycline and •2mL Water/Acetonitrile (60/40) 4-epioxytetracycline (4-epiOTC) at 50µg/L (blue) or not spiked (red) or of 1.5mL of water spiked with Drying 3 minutes Tetracycline, Chlortetracycline and 4- Elution (E) epioxytetracycline at 50µg/L (pink) 2mL Methanol with 2% Formic acid Recovery of Tetracyclines after AFFINIMIP® SPE The elution fraction was then evaporated and Tetracyclines clean-up of Salmon or milk spiked at dissolved in the mobile phase before HPLC analysis. 50 or 100µg/L and relative standard deviation calculated from results generated under HPLC Method with UV detection repeatability conditions (n=3). Column: Hypersil Gold C18 column 150mm x 2.1mm, 3µm Molecules Mean Milk Salmon Mobile phase: gradient profile (µg/L) R % % RSDr R% Tetracycline 49.6 99.4 4.9 113 Oxytetracycline 45.6 91.3 7.1 - Chlortetracycline 37.2 74.4 6.3 74 4-epitetracycline 47.9 95.9 5.1 - (4-epiTC) 4- 108.4 108.4 15.0 97 epichlortetracycline 4- 43.7 87.4 9.1 71 epioxytetracycline 88.0 2.9 89 Doxycycline (DOX) 43.8 Time ( % 10mM % 10mM % MeOH Catalog number: min) Oxalic Acid Oxalic Acid 1mL-10mg sorbent 5 PI-FS112-02A for 25 cartridges 1mL 0 Water ACN 5 PI-FS112-03A for 50 cartridges 1mL 20 10 kit of 12 reservoirs 15ml and adapters for 21 90 5 10 use with 1, 3 & 6 mL columns 40 90 5 5 PI-ACC-AR2 41 80 10 80 10 90 5 Flow rate: 0.2mL/min UV detection: 355nm Injection volume: 100µL. 56
DG101 AFFINIMIP® SPE Metanephrines Quantification of free Metanephrines in MeO MeO Plasma is considered to be the most accurate test for the clinical chemical diagnosis of OH OH Pheocromocytoma. The concentrations of these endogenous HO Me HO molecules are very low in serum and plasma N (lower than 1nM). A clean up step is crucial in H NH2 order to improve the sensitivity and the specificity before LC analysis. Metanephrine (MN) Normetanephrine (NMN) MeO HO NH2 3-Methoxytyramine (3-MT) Current method involves non specific sample preparation. To propose an accurate solution, we have developed AFFINIMIP® SPE Metanephrines cartridges, a powerful technique for clean-up and pre-concentration applications of Metanephrines. This study describes the solid phase extraction of Metanephrines from plasma sample using AFFINIMIP® SPE Metanephrines. In addition, a comparison with a Weak Cation Exchange SPE cartridge is shown. Catalog number: 1mL cartridge PI-DG101-02A for 25 cartridges PI-DG101-03A for 50 cartridges 3mL cartridge PI-DG101-02 for 25 cartridges PI-DG101-03 for 50 cartridges 57
AFFINIMIP® SPE Metanephrines DETERMINATION OF METANEFHRINES IN PLASMA COMPARISON WITH WCX CARTRIDGES PROTOCOL OF CLEANUP RESULTS Sample preparation The plasma or serum is diluted by 5 with water. WCX AFFINIMIP® SPE This solution is used as the loading solution. Cleanup with a 1mL AFFINIMIP® SPE Metanephrines cartridge Equilibration Analysis by LC-MS/MS: Total Ion Current of a calf •1mL of phosphate buffer pH 7 serum after Cleanup by AFFINIMIP® SPE •2mL Water Metanephrines. The sample naturally contained Metanephrine. Concentration of MN found: 30nM. Loading In parallel, a SPE was performed on a protocol 1.5mL of loading solution developed for the analysis of MN using WCX cartridges: the concentration obtained was 7nM for Washing of interferents (W1) the same sample. •1mL Water •500µL Water/Methanol (60/40) WCX Recovery : 33% Drying 10 seconds Washing of interferents (W2) NMN •500µL Methanol Elution (E) 1mL Methanol – 5% Acetic acid The elution fraction was then evaporated and dissolved in the mobile phase before HPLC analysis. AFFINIMIP SPE Recovery: 101% Analysis by LC-MS/MS: Selected ion monitoring of Normetanephrine (m/z 180). Chromatograms obtained after Cleanup by AFFINIMIP® SPE Metanephrines or by WCX of a calf serum spiked at 27nM with Normetanephrine. Recoveries of MN and NMN at a contamination level of 500nM in rabbit plasma after AFFINIMIP® SPE Metanephrines Clean-up and relative standard deviation calculated from results generated under reproducibility conditions (Analysis by LC-MS). Analytes Recoveries % % RSDR Metanephrine 79.4 6.3 Normetanephrine 109 11 HPLC Method with LC-MS/MS detection Catalog number: Column: Syncronis aQ column 150mm x 2.1mm 1mL cartridge Mobile phase: Water – 0.1% Formic Acid PI-DG101-02A for 25 cartridges flow rate: 0.2mL/min PI-DG101-03A for 50 cartridges MS detection: m/z 322 (ESI+) 3mL cartridge Injection volume: 20µL. PI-DG101-02 for 25 cartridges PI-DG101-03 for 50 cartridges 58
AFFINIMIP® SPE Phenolics FS103 Class-selective extraction of Phenolic compounds from a variety samples: water, cosmectics, biological or food matrices. Phenolic compounds are important families of products found as natural substances in plants and life sciences or as synthetic products such as drugs. AFFINIMIP® SPE Phenolics are class-selective solid phase extraction cartridges that selectively clean and concentrate a broad range of phenolic compounds prior to analysis. This treatment strongly reduces the amount of interferents and the matrix effects. Benefits •Extraction of a broad family of phenolic compounds •Reduction of matrix effects and of most interferents •Several protocols proposed to purify your product Catalog number: 3mL-100mg sorbent PI-FS103-02 for 25 cartridges PI-FS103-03 for 50 cartridges 59
AFFINIMIP® SPE Phenolics DETERMINATION OF PARABENS IN COSMETIC PRODUCTS RESULTS 1200 1200 1100 1100 Before1000 1000 900 900 800 800 700 After 700 mAU 600 600 mAU 500 500 400 400 General structure of Parabens 300 300 PROTOCOL OF CLEANUP 200 200 Sample preparation 100 100 1g of Lotion was mixed 1minute with 1mL of H2SO4 2M and 50mL of 90/10 Ethanol/Water. 00 The mixture was heated during 5min at 60°C. Then the solution is filtered on filter paper (4- 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 7µm). This extract was diluted by 3 with water. Minutes The solution was spiked with methylparaben to simulate a concentration of paraben in the Chromatograms of a cream containing 0.2% of lotion at 0.2%, 0.4% and 0.8%. methylparaben before clean-up (blue) and after clean-up (Red) with AFFINIMIP® SPE Phenolics. Cleanup with a 3mL/100mg AFFINIMIP® SPE Phenolics cartridge 1800 1800 Equilibration Cream spiked at 0.8%, •3mL Acetonitrile •3mL Water 1600 yield 91% (n=2) 1600 Loading 1400 1400 •Up to 5mL of loading solution 1200 Cream spiked at 0.4%, 1200 Washing of interferents •3mL Water / Acetonitrile (75/25 v/v) yield 106 (n=2) Elution (E) 1000 1000 •3mL Methanol The elution fraction was diluted by 2 with mAU mAU water prior to analysis. 800 Cream spiked at 0.2%, 800 600 yield 106 (n=2) 600 400 400 200 200 00 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 Minutes Chromatograms obtained after clean-up with AFFINIMIP® SPE Phenolics of a cream (without parabens) spiked with different concentrations of methylparaben Recovery yields and reproducibility after AFFINIMIP® SPE Phenolics Clean-up. Recoveries % (n=6) RSDR% 101.1 8 HPLC-UV Method Catalog number: Column: Thermo Hypersil gold, 150mm x 2.1mm 3mL-100mg sorbent Mobile phase: 60/40 (v/v) Water/Methanol PI-FS103-02 for 25 cartridges Flow rate: 0.2mL/min PI-FS103-03 for 50 cartridges Detection: UV - 254nm Injection volume: 20µL. 60
AFFINIMIP® SPE Phenolics DETERMINATION OF GUAIACOL RESULTS 350 350 300 300 250 250 200 200 mAU mAU General structure of Guaîacol Guaïacol150 150 PROTOCOL OF CLEANUP 100 100 Cleanup with a 3mL/100mg AFFINIMIP® SPE Phenolics cartridge 50 50 Equilibration 00 •3mL Acetonitrile •3mL Water -50 -50 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Loading Minut es •Up to 2mL of red or white wine Chromatograms obtained after clean-up with Washing of interferents AFFINIMIP® SPE Phenolics of red wine spiked with •3mL Water / Acetonitrile (80/20 v/v) Guaïacol (0.1µM) (red) or not spiked (blue). Elution (E) 2600 •2mL Methanol 2400 HPLC-UV Method Column: Thermo Hypersil gold, 150mm x 4.6mm 2200 Mobile phase: 15/85 (v/v) Acetonitrile Water 2000 Flow rate: 1mL/min Detection: UV - 272nm 1800 Injection volume: 100µL. 1600 1400 1200 mAU 1000 Guaïacol 800 600 400 200 0 -200 200 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Minut es Chromatograms obtained before (red) and after (blue) clean-up with AFFINIMIP® SPE Phenolics of red wine spiked with Guaïacol (0.1µM) Recovery yields and reproducibility evaluated with 3 cartridges and 3 different batches of AFFINIMIP® SPE Phenolics by matrix (n=9) Red wine 1 C° (µM) Recoveries % RSDR% 0.1 88.1 3.9 Red wine 2 0.1 93.1 3.7 White wine 1 0.02 96.8 1.7 White wine 2 0.02 93.5 2.6 Catalog number: 3mL-100mg sorbent PI-FS103-02 for 25 cartridges PI-FS103-03 for 50 cartridges 61
AFFINIMIP® SPE Phenolics DETERMINATION OF CARNOSIC ACID IN MEAT RESULTS UV 6000-230nm 450 E ZON ethanol.dat 450 400 400 Carnosic350 350 acid300 300 mAU 250 250 mAU 200 200 structure of Carnosic acid 150 150 PROTOCOL OF CLEANUP 100 100 Sample preparation 50 50 25g of turkey was mixed with 200mL of 74.5/25/0.5 ACN/H2O/H3PO4 or Ethanol-0.5% 00 H3PO4 using a blender during 3 minutes. After, the mixture was mixed during 30 minutes with 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 magnetic stirrer. The mixture was filtered on Minutes filter paper (4-7µm). Then the mixture was diluted by 2 with water. Chromatogram of a turkey containing 50ppm of Carnosic acid after clean-up with AFFINIMIP® SPE Cleanup with a 3mL/100mg AFFINIMIP® SPE Phenolics. Extraction of the turkey with Ethanol- Phenolics cartridge 0.5% H3PO4 Equilibration UV 6000-230nm 500 •3mL Acetonitrile 500 E ZON 75-25.dat •3mL Water 450 450 Loading •Up to 80mL of loading solution 400 400 Washing of interferents Carnosic •3mL Water / Acetonitrile (60/40 v/v) 350 350 Elution (E) •2mL Methanol -1% H3PO4 acid 300 300 mAU 250 250 mAU 200 200 150 150 100 100 50 50 00 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Minutes Chromatogram of a turkey containing 50ppm of Carnosic acid after clean-up with AFFINIMIP® SPE Phenolics. Extraction of the turkey with 74.5/25/0.5 ACN/H2O/H3PO4 Recovery yields obtained by both extraction solvent after AFFINIMIP® SPE Phenolics Clean-up. Extraction solvent Recoveries % 74.5/25/0.5 ACN/H2O/H3PO4 >85% >80% Ethanol-0.5% H3PO4 HPLC-UV Method Catalog number: Column: Thermo Hypersil gold, 150mm x 4.6mm 3mL-100mg sorbent Mobile phase: 65/35 (v/v) ACN/Water-0.5% H3PO4 PI-FS103-02 for 25 cartridges Flow rate: 1mL/min PI-FS103-03 for 50 cartridges Detection: UV - 230nm Injection volume: 5µL. 62
AFFINIMIP® SPE PRODUCT LIST FOR MYCOTOXINS ANALYSES Products Designation Definition Reference Nber of cartridges Zearalenone and AFFINIMIP® SPE 3mL Selective SPE cartridges for PI-FS109-02 25 Fumonisins FumoZON Zearalenone and Fumonisins PI-FS109-03 50 PI-FS114-03 50 Multimycotoxins AFFINIMIP® SPE 3mL Selective SPE cartridges for analyses of 100 Multimyco10 Aflatoxins, Zearalenone, Ochratoxin A, T-2, PI-FS114-04 HT-2, Fumonisins 3mL – 100mg Selective SPE cartridges for PI-FS102-02 25 Patulin PI-FS102-03 50 PI-FS102-02B-200mg 25 AFFINIMIP® SPE Patulin PI-FS102-03B-200mg 50 6mL – 200mg Selective SPE cartridges for Patulin (for DRIED APPLE and higher enrichment with apple juice) Patulin PI-FS102-02K 25 PI-FS102-03K 50 Kit of 3mL selective SPE cartridges for PI-FS102-02KB-200mg 25 Patulin + 50mL Pectinase enzyme solution PI-FS102-03KB-200mg 50 AFFINIMIP® SPE Patulin & Pectinase kit Kit of 6mL - 200mg selective SPE cartridges for Patulin in dried apple + 50mL Pectinase enzyme solution AFFINIMIP® SPE 3mL Selective SPE cartridges for Ochratoxin PI-FS101-02 25 Ochratoxin A A PI-FS101-03 50 25 Ochratoxin A AFFINIMIP® SPE 6mL Selective SPE cartridges for Ochratoxin PI-FS101-02B 50 Deoxynivalenol Deoxynivalenol A PI-FS101-03B 25 50 6mL -100mg Selective SPE cartridges for PI-FS117-02B 25 Deoxynivalenol in FOOD PI-FS117-03B 50 6mL -200mg Selective SPE cartridges for PI-FS101-02B-200mg Deoxynivalenol in ANIMAL FEED PI-FS101-03B-200mg Pectinase 50 mL Pectinase enzyme solution PI-REA-001-50mL Zearalenone AFFINIMIP® SPE 3mL Selective SPE cartridges for ZON PI-FS100-02 25 Zearalenone PI-FS100-03 50 For other formats, please contact us AFFINISEP can provide you with other formats than the one described in the product list. Other formats available on demand and with an adapted protocol can be : • different volumes of SPE cartridges (1mL, 3mL, LRC, 60mL, etc… ) • 96 well plates, • HPLC columns, • Preparative HPLC columns • the format adapted to your application and your automate 63
AFFINIMIP® SPE PRODUCT LIST (MISCELLANEOUS) Products Designation Definition Reference Nber of cartridges Amphetamines AFFINIMIP® SPE 3mL Selective SPE cartridges for PI-DG102-02 Amphetamines Amphetamines derivatives PI-DG102-03 25 PI-FS106-02 50 3mL Selective SPE cartridges for PI-FS106-03 25 Bisphenol A (PP) PI-FS106-02B 50 PI-FS106-03B 25 Bisphenol A AFFINIMIP® SPE 6mL Selective SPE cartridges for PI-FS106-02G 50 PI-FS106-03G 25 Bisphenol A Bisphenol A (PP) PI-DG100-02 50 PI-DG100-03 25 6mL Selective SPE cartridges for PI-DG100-02A 50 Bisphenol A (Glass) PI-DG100-03A 25 PI-FS110-02A 50 Catecholamines AFFINIMIP® SPE 3mL Selective SPE cartridges for PI-FS110-03A 25 Catecholamines Catecholamines PI-FS104-02A 50 PI-FS104-03A 25 1mL Selective SPE cartridges for PI-FS104-02 50 Catecholamines PI-FS104-03 25 PI-FS115-02 50 Chloramphenicol AFFINIMIP® SPE 1mL Selective SPE cartridges for 25 Chloramphenicol Chloramphenicol PI-FS115-03 50 Estrogens AFFINIMIP® SPE 1mL Selective SPE cartridges for PI-DG101-02 Estrogens Estrogens PI-DG101-03 25 PI-DG101-02A 50 Picolinic acid AFFINIMIP® SPE 3mL Selective SPE cartridges for PI-DG101-03A 25 based herbicides Picolinic Estrogens 50 Herbicides Selective SPE cartridges for Picolinic AFFINIMIP® SPE acid based herbicides Metanephrines Metanephrines 3mL Selective SPE cartridges for Phenolics AFFINIMIP® SPE Metanephrines PI-FS103-03 50 Tamoxifen Phenolics Tetracyclines 1mL Selective SPE cartridges for PI-PH101-02 25 AFFINIMIP® SPE Metanephrines Tamoxifen PI-FS112-02A 25 3mL Selective SPE cartridges for PI-FS112-03A 50 AFFINIMIP® SPE Phenolic compounds PI-FS105-02 25 Tetracyclines PI-FS105-03 50 3mL Selective SPE cartridges for Tamoxifen 1mL Selective SPE cartridges for Tetracyclines Zeranol Residues AFFINIMIP® SPE 3mL Selective SPE cartridges for Zeranol Residues Zeranol Residues
LIST OF PUBLICATIONS AND POSTERS Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals, J. Chrom. A., 1217, 6668-6673, 2010. Molecularly imprinted polymer solid-phase extraction for detection of zearalenone in cereal sample extracts detection, Analytica Chimica Acta, 672, 15–19, 2010. Molecularly imprinted polymer as sorbent in micro-solid phase extraction of ochratoxin A in coffee, grape juice and urine, Tien Ping Lee, Bahruddin Saad, Wejdan Shakir Khayoon, Baharuddin Salleh, Talanta, 88, 129–135, 2012. Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in ginger and LC-MS/MS confirmation, J. Cao, S. Zhou, W. Kong, M. Yang, L. Wan, S. Yang, Food control, 33(2), 337-343, 2013. Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in beer, red wine, and grape juice, J. Cao, W. Kong, S. Zhou, L. Yin, L. Wan, M. Yang, J. Sep. Sci., 36(7), 1291- 1297, 2013. Application of molecularly imprinted polymers to determine B1, B2, and B3 fumonisins in cereal products, M. Bryła, R. Jedrzejczak, M. Roszko, K. Szymczyk, M. W. Obiedziński, J. Sękul, M. Rzepkowska, J. Sep. Sci., 36(3), 578-584, 2013. Breakthrough innovation in rapid detection kits using MOLECULARLY IMPRINTED POLYMERS SPE for an early quantification of PATULIN from apple-based food matrices, D. Derrien, C. Pérollier, O. Lépine, K. Naraghi, S. Bayoudh, poster at 7th World Mycotoxin Forum, Rotterdam, The Netherlands, November 5-9, 2012. Molecularly Imprinted Polymer for Solid Phase Extraction of Patulin mycotoxin, D. Derrien, M. Mulet, F. Alix, C. Pérollier, O. Lépine, K. Naraghi, S. Bayoudh, 33rd Mycotoxin Workshop, Freising, Germany, 30 May - 1 June 2011 Interest of molecularly imprinted polymers in the fight against doping. Extraction of tamoxifen and its main metabolite from urine followed by high-performance liquid chromatography with UV detection. J. Chrom. A, 1196–1197, 81–88, 2008. Analysis of urinary neurotransmitters by capillary electrophoresis: Sensitivity enhancement using field-amplified sample injection and molecular imprinted polymer solid phase extraction, B. Claude , R. Nehmé, P. Morin, Analytica Chimica Acta, 699 (2), 242–248, 2011. Solid-phase extraction using molecularly imprinted polymer for selective extraction of natural and synthetic estrogens from aqueous samples, P. Lucci, O. Núñez, M.T. Galceran, J. Chrom. A, 1218, 4828-4833, 2011. On-line molecularly imprinted solid-phase extraction coupled to liquid chromatography-tandem mass spectrometry for the determination of hormones in water and sediment samples, D. Matějíček, J. Vlček, A. Burešová, P. Pelcová, J. Sep. Sci., 36(9-10), 1509-1515, 2013. Quantification of estrogens at ppt levels in bovine plasma by Molecularly Imprinted Solid Phase Extraction and GC-MS/MS analysis, S. Rochereau, E. Bichon, F. Courant, F. Monteau, S. Prévost, F. Hanganu, N. Cesbron, G. Dervilly-Pinel, B. Le Bizec (LABERCA), Poster Euroresidues VIIth conference, 2012 . The use of molecularly imprinted polymers for the multicomponent determination of endocrine- disrupting compounds in water and sediment, D. Matějíček, A. Grycová, J. Vlček, J. Sep. Sci., 36(6), 1097-1103, 2013. 66
LIST OF PUBLICATIONS AND POSTERS Molecularly imprinted polymer applied to the selective isolation of urinary steroid hormones: An efficient tool in the control of natural steroid hormones abuse in cattle, M. Doué, E. Bichon, G. Dervilly-Pinel, V. Pichon, F. Chapuis-Hugon, E. Lesellier, C. West, F. Monteau, B. Le Bizec, J. Chrom A, 1270, 51-56, 2012. High efficiency of semi-preparative Supercritical Fluid Chromatography with Molecularly Imprinted Polymer as stationary phase (SFC-MIP). Application on urinary steroids purification for IRMS analysis, M. Doué, E. Bichon, F. Monteau, B. Le Bizec (LABERCA), poster 2nd International Symposium on HTSP 2012. New technological tools for isolating and measuring growth promoting agents in edible tissues and biological fluids, E. Bichon, S. Rochereau, L. Seree, S. Prevost, F. Monteau, B. Le Bizec (LABERCA) Poster session RAFA 2011. Utilisation de la spectrométrie de masse pour le dosage du Bisphénol A dans les matrices alimentaires, Y. DECEUNINCK, Z. ZENDONG, E. BICHON, J.-P. ANTIGNAC, B. LE BIZEC (LABERCA) poster SMAP 2011, Avignon, France, 19-22 sept. 2011. Quantitative analysis of Bisphenol A in all liquid or solid food matrices were carried out by using AFFINIMIP® SPE Bisphenol A (p132, Annex 12 of Annexes to the report on the assessment of the risks associated with bisphenol A (BPA) for human health, and on toxicological data and data on the use of bisphenols S, F, M, B, AP, AF, and BADGE (In French)), ANSES April 2013. A high selective and sensitive liquid chromatography–tandem mass spectrometry method for quantization of BPA urinary levels in children, C. Nicolucci, S. Rossi, C. Menale, E. Giudice, P. Miraglia del Giudice, L. Perrone, P. Gallo, D. Mita, N. Diano, Analytical and Bioanalytical Chemistry, 1618- 2642, 2013. Molecularly imprinted polymers-liquid chromatography/fluorescence for the selective clean-up of hydroxylated polycyclic aromatic hydrocarbons in soils, O. Baltrons, M. Lopez-Mesas, C. Palet, F. Lederf and F. Portet-Koltalo, Anal. Methods, 2013,5, 6297-6305 Fast and Selective Purification of a Fluorous Radiotracer using AFFINIMIP® SPE 18F based on Molecularly Imprinted Polymers SPE, C. Pérollier, F. Alix, S. Bayoudh, K. Naraghi, Poster 19th ISRS 2011. Rapid Purification of Fluorine-18 Containing Synthons Using Molecularly Imprinted Polymer Cartridges, G. Smith, S. Bayoudh, C. Pérollier, R. Bhalla, International Symposium on Radiopharmaceutical Sciences (ISRS), Jeju, Korea, 12-17 May 2013. Works carried out in collaboration with GE Healthcare. Selective solid phase extraction of catecholamines and metanephrines from serum using a new molecularly imprinted polymer, B. Claude, P. Morin and L. Denoroy, J. Liquid Chromatography & Related Technologies, 2014, 37 (18), 2624-2638. 18F-Anti-MMR-nanobodies for PET imaging of tumor-associated macrophage subtypes as surrogate markers for tumor hypoxia. A. Blykers, C. Xavier, I. Vaneycken, D. Laoui, N. Devoogdt, M. D’huyvetter, T. Lahoutte, J. A. Van Ginderachter and V. Caveliers, poster at European Molecular Imaging Meeting in Turin (2013). A developmental hepatotoxicity study of dietary bisphenol A in Sparus aurata juveniles, F. Maradonna, V. Nozzi, L. Dalla Valle, I. Traversi, G. Gioacchini, F. Benato, E. Colletti, P. Gallo, I. Di Marco Pisciottano, D. G. Mita, G. Hardiman, A. Mandich, O. Carnevali, Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2014, 166, 1–13. 67
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