MonoSpin

Monolithic SPE Column for the Purification and Enrichment of Small Amount Sample MonoSpin® Series MonoSpin is a monolithic SPE column excellent for the small volume sample with an easy and quick operation by centrifuge. <Features> ◆ Easy operation by centrifuge ◆ Speedy sample treatment with a superb Monolithic Silica Disk through pore ◆ Excellent for the sample for 50-800μL ◆ Selective purification/enrichment by various functional groups MonoSpin Monolithic Silica Enlarged Figure <How to Use MonoSpin> Centrifuge Centrifuge Centrifuge Centrifuge Equilibrating Adsorption Wash Elute Centrifuge Operation Monolith MonoSpin® C18 Octadecyl functional group. Monolith MonoSpin® SAX Bonded with Trimethyl Optimal for drug extraction in aminopropyl combining both (CH2)17CH3 biological samples, and desalting Cl strong anion exchange & weak & enrichment of peptide samples. hydrophobic interaction. + Optimal for the extraction of Monolith MonoSpin® NH2 Bonded with aminopropyl. Monolith acidic drugs. (CH2)3 NH2 Optimal for the enrichment of (CH2)3 N (CH3)3 sugar chain and/or hydrophilic Specific column combined with compounds by HILIC mode. MonoSpin® PBA phenyl boric acid. Excellent for the selective extraction of cis MonoSpin® SCX Bonded with propyl benzene N diol compounds, such as H catechol amines. Monolith (CH2)3 - sulfonic acid combining both Monolith SO3 strong cation exchange & B(OH)2 Monolith skeleton coated with hydrophobic interaction. Optimal dioxide titanium. Excellent for MonoSpin® TiO the enrichment of phosphopeptides TiO2 for the extraction of basic drugs. MonoSpin® Amide Bonded with amide group. MonoSpin® CBA Optimal for the extraction of sugar chains and various acidic and Bonded with propyl benzene basic hydrophilic compounds by sulfonic acid combining both HILIC mode. strong cation exchange & hydrophobic interaction. Optima for the extraction of basic drugs. <Product Information> Qty Cat.No. Description Qty Cat.No. Description 50 5010-21700 MonoSpin PBA 50 5010-21715 100 5010-21701 MonoSpin TiO 100 5010-21716 MonoSpin C18 50 5010-21710 MonoSpin Amide 50 5010-21705 100 5010-21711 MonoSpin CBA 100 5010-21706 MonoSpin NH2 50 5010-21725 50 5010-21727 100 5010-21726 100 5010-21728 MonoSpin SCX 50 5010-21720 50 5010-21729 100 5010-21721 100 5010-21730 MonoSpin SAX

【Application】 Pyridyl Aminated Suger Chain (MonoSpin® NH2) - No special system is required (A) PA001(TaKaRa BIO) (B) Purify PA001 - Large amount sample (up to 500ug) 0.01 pmol - Flexible elution buffer Drug in Urine (MonoSpin® C18) (A) Urine Sample 80 80 60 60 (B) Purify with MonoSpin C18 after adding drug to urine FL FL 40 40 20 20 0 0 Drug： Add 5ug/mL each 0 2 4 6 8 10 0 2 4 6 8 10 MA(Methamphetamine), MDMA(3,4-Methylenedioxymethamphetamine） AP(Amphetamine), MDA(3,4-methylenedioxyamphetamine) Time (min) Time (min) Each drug was enriched by the hydrophobic interaction of C18 Purify pyridyl aminated sugar chain by NH2 HILIC ＜J. Chromatogr A 1208 (2008) 71-75＞ mode No sample decrease is observed by retention nor purification. Catechol Amines in Urine (MonoSpin® PBA) Phospholic amino Herbicides in Urine （MonoSpin® TiO) (A) Urine Sample+1μg/mL DHBA Sample (A) Herbicides in Urine (B) Purification of 1 : Noradrenaline (1ppm each) herbicides in urine 2 : Adrenalin nA 3 : DHBA Bialaphos 100 200 4 : Dopamine 0 10 20 Glyphosate MPPA Time (min) Glufosinate (B) Purify urine with MonoSpin PBA and enrich the AMPA concentration by 5 times Phospholic amino herbicides were selectively enriched 3 and removed contaminants in urine by TiO. nA 1 4 100 200 2 0 10 20 Time (min) Catechol amines were enriched by PBA