HPLC/UHPLC Columns Inertsil® SIL-150A Physical Properties HPLC/UHPLC Columns Silica : 2 Series High Purity Silica Gel Particle Size : 5 μm Surface Area : 320 m2/g Pore Size : 150 Å Pore Volume : 1.20 mL/g Bonded Phase : None End-capping : None Carbon Loading : - % USP Code : L3 pH Range : 2-7.5 Inertsil SIL-150A is ultra pure silica gel column, and this ultra pure silica gel contains very low levels of metal impurities and is durable and free from dents and cracks which can cause premature column failure. Compared to Inertsil SIL-100A, the silica’s surface area is smaller (320 m²/g). Retentivity of Inertsil SIL-150A is weaker than that of Inertsil SIL-100A. Dimensions Inertsil SIL-150A Guard Column E (200 bar) 5 μm ID x Length (in mm) Reusable holder with universal (200 bar) self adjusting connections and 2.1 x 250 cartridges containing the same 2.1 x 150 IN5020-01025 high performance packing 3.0 x 250 IN5020-01021 material 3.0 x 150 IN5020-01026 4.0 x 250 IN5020-01022 4.0 x 150 IN5020-01027 4.6 x 250 IN5020-01023 4.6 x 150 IN5020-01028 IN5020-01024 Inertsil SIL-150A Guard Columns Dimensions 5 μm 5 μm Column Replacement ID (mm) ID x Length (mm) (Guard Column E Set) *Includes 1 holder Cartridges + 2 cartridges 3.0 x 10 IN5020-19189 IN5020-19139 2.1, 3.0 4.0 x 10 IN5020-19089 IN5020-19039 4.0, 4.6 3.0 x 20 IN5020-19589 IN5020-19539 2.1, 3.0 4.0 x 20 IN5020-19489 IN5020-19439 4.0, 4.6 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-51 LIFE SCIENCE
Colonnes HPLC/UHPLC Inertsil® HILIC Colonnes HPLC/UHPLC Physical Properties Silica : 3 Series High Purity Silica Gel Particle Size : 3 μm, 5 μm Surface Area : 450 m2/g Pore Size : 100 Å Pore Volume : 1.05 mL/g Bonded Phase : Diol Groups (Dihydroxypropyl Groups) End-capping : None Carbon Loading : 20% USP Code : L20 pH Range : 2-7.5 HILIC is the abbreviated name for Hydrophilic Interaction Chromatography. It was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when organic concentration is raised, it shows a strong retention. Moreover, it can get more stable analysis by adding a basic solvent to the eluate, such as ammonium acetate. Separation mode from Reverse phase to HILIC ODS column ODS column ODS column HILIC column HILIC column Reverse phase mode pH control Using Ion pair reagent Using higher organic solvent Add buffer No retention No retention No retention Improve to a stable condition 1. Allantoin Reproducibility of adjust eluent 1 1 1 1 1 first time 2nd time 3rd time 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 Time(min) Time(min) Time(min) Time(min) Time(min) Conditions Conditions Conditions Conditions Conditions Column : ODS Column : ODS Column : ODS Column : Inertsil HILIC Column : Inertsil HILIC Eluent : A) CH3CN Eluent : A) CH3CN Eluent : A) CH3CN Eluent : A) CH3CN Eluent : A) CH3CN B) H2O B) 10 mM phosphate buffer(pH 7.5) B) IPCC-04 B) H2O B) 10 mM CH3COONH4 A/B = 5/95, v/v A/B = 5/95, v/v A/B = 5/95, v/v A/B = 95/5, v/v A/B = 95/5, v/v E-52 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE
Dimensions Inertsil HILIC 5 μm HPLC/UHPLC Columns HPLC/UHPLC Columns 3 μm (200 bar) ID x Length (in mm) (200 bar) Inertsil® HILIC IN5020-86716 1.0 x 250 IN5020-86736 IN5020-86715 Guard Column E (200 bar) 1.0 x 150 IN5020-86735 IN5020-86714 1.0 x 100 IN5020-86734 IN5020-86713 Reusable holder with universal self adjusting 1.0 x 75 IN5020-86733 IN5020-86712 connections and cartridges containing the same 1.0 x 50 IN5020-86732 IN5020-86711 1.0 x 33 IN5020-86731 IN5020-07706 high performance packing material 2.1 x 250 IN5020-07766 IN5020-07705 2.1 x 150 IN5020-07765 IN5020-07704 Did you know? 2.1 x 100 IN5020-07764 IN5020-07703 Canadian Life Science is an 2.1 x 75 IN5020-07763 IN5020-07702 Authorized Distributor for 2.1 x 50 IN5020-07762 IN5020-07701 Honeywell Research Chemicals? 2.1 x 33 IN5020-07761 IN5020-07716 3.0 x 250 IN5020-07776 IN5020-07715 For more information please 3.0 x 150 IN5020-07775 IN5020-07714 ask your Sales Representative today. 3.0 x 100 IN5020-07774 IN5020-07713 3.0 x 75 IN5020-07773 IN5020-07712 3.0 x 50 IN5020-07772 IN5020-07711 3.0 x 33 IN5020-07771 IN5020-07726 4.0 x 250 IN5020-07786 IN5020-07725 4.0 x 150 IN5020-07785 IN5020-07724 4.0 x 100 IN5020-07784 IN5020-07723 4.0 x 75 IN5020-07783 IN5020-07722 4.0 x 50 IN5020-07782 IN5020-07721 4.0 x 33 IN5020-07781 IN5020-07736 4.6 x 250 IN5020-07796 IN5020-07735 4.6 x 150 IN5020-07795 IN5020-07734 4.6 x 100 IN5020-07794 IN5020-07733 4.6 x 75 IN5020-07793 IN5020-07732 4.6 x 50 IN5020-07792 IN5020-07731 4.6 x 33 IN5020-07791 Inertsil HILIC Guard Columns Dimensions 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement ID (mm) ID x Length (mm) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 1.0 x 10 IN5020-19275 IN5020-19225 IN5020-19274 IN5020-19224 3.0 1.5 x 10 IN5020-19375 4.0, 4.6 3.0 x 10 IN5020-19175 IN5020-19325 IN5020-19374 IN5020-19324 2.1, 3.0 4.0 x 10 IN5020-19075 4.0, 4.6 3.0 x 20 IN5020-19575 IN5020-19125 IN5020-19174 IN5020-19124 4.0 x 20 IN5020-19475 IN5020-19025 IN5020-19074 IN5020-19024 IN5020-19525 IN5020-19574 IN5020-19524 IN5020-19425 IN5020-19474 IN5020-19424 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-53 LIFE SCIENCE
Colonnes HPLC/UHPLC Inertsil® AX Colonnes HPLC/UHPLC Physical Properties Silica : 3 Series High Purity Silica Gel Particle Size : 5 μm Surface Area : 450 m2/g Pore Size : 100 Å Pore Volume : 1.05 mL/g Bonded Phase : Diethylaminopropyl Groups End-capping : None Inertsil AX Carbon Loading : 17% Dimensions 5 μm (200 bar) USP Code :- ID x Length (in mm) pH Range : 2-7.5 1.0 x 250 IN5020-80116 1.0 x 150 IN5020-80115 Inertsil AX has diethylamino groups bonded to silica gel by an alkyl chain. The diethylamino 1.0 x 100 IN5020-80114 groups offer anionic functions required for anion exchange chromatography. It is mainly used for analyses of acidic compounds. Conventional ion-exchange columns used to show 1.0 x 75 IN5020-80113 inconsistent results from lot to lot. However, Inertsil AX is manufactured under strict quality control in order to offer excellent lot-to-lot reproducibility. The retentivity of Inertsil AX 1.0 x 50 IN5020-80112 is influenced by the concentration of buffer. The retention time can be adjusted by the concentration of buffer. 1.0 x 33 IN5020-80111 2.1 x 250 IN5020-07216 2.1 x 150 IN5020-07215 2.1 x 100 IN5020-07214 2.1 x 75 IN5020-07213 2.1 x 50 IN5020-07212 Guard Column E (200 bar) 2.1 x 33 IN5020-07211 Reusable holder with universal 3.0 x 250 IN5020-07226 self adjusting connections and cartridges containing the same 3.0 x 150 IN5020-07225 high performance packing material 3.0 x 100 IN5020-07224 3.0 x 75 IN5020-07223 3.0 x 50 IN5020-07222 3.0 x 33 IN5020-07221 4.0 x 250 IN5020-07236 Inertsil AX Guard Columns 4.0 x 150 IN5020-07235 Dimensions 5 μm 5 μm Column 4.0 x 100 IN5020-07234 Replacement ID (mm) ID x Length (mm) (Guard Column E Set) 4.0 x 75 IN5020-07233 *Includes 1 holder Cartridges + 2 cartridges 4.0 x 50 IN5020-07232 1.0 x 10 IN5020-19283 IN5020-19233 1.0 4.0 x 33 IN5020-07231 1.5 x 10 IN5020-19383 IN5020-19333 1.5, 2.1 4.6 x 250 IN5020-07246 3.0 x 10 IN5020-19183 IN5020-19133 3.0 4.6 x 150 IN5020-07245 4.0 x 10 IN5020-19083 IN5020-19033 4.0, 4.6 4.6 x 100 IN5020-07244 3.0 x 20 IN5020-19583 IN5020-19533 2.1, 3.0 4.6 x 75 IN5020-07243 4.0 x 20 IN5020-19483 IN5020-19433 4.0, 4.6 4.6 x 50 IN5020-07242 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 4.6 x 33 IN5020-07241 E-54 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE
HPLC/UHPLC Columns Inertsil® CX Physical Properties HPLC/UHPLC Columns Silica : 3 Series High Purity Silica Gel Particle Size : 5 μm Surface Area : 450 m2/g Pore Size : 100 Å Pore Volume : 1.05 mL/g Bonded Phase : Benzensulfonyl Groups End-capping : None Inertsil CX Carbon Loading : 14% Dimensions 5 μm (200 bar) USP Code : L9 ID x Length (in mm) pH Range : 2-7.5 1.0 x 250 IN5020-80016 1.0 x 150 IN5020-80015 Inertsil CX has benzenesulfonyl groups bonded to silica gel by an alkyl chain. The sulfonyl 1.0 x 100 IN5020-80014 groups at the end of the structure offer cationic functions required for the cation exchange chromatography. It is mainly used for analyses of basic compounds. Inertsil CX is manufactured 1.0 x 75 IN5020-80013 under strict quality control in order to offer excellent lot-to-lot reproducibility as the same as Inertsil AX. Inertsil CX has high ion exchange capacity and provides high retentivity and 1.0 x 50 IN5020-80012 selectivity. Therefore, it is also suited for analyzing amino acids and nucleobases. 1.0 x 33 IN5020-80011 2.1 x 250 IN5020-07116 2.1 x 150 IN5020-07115 2.1 x 100 IN5020-07114 2.1 x 75 IN5020-07113 2.1 x 50 IN5020-07112 Guard Column E (200 bar) 2.1 x 33 IN5020-07111 Reusable holder with universal 3.0 x 250 IN5020-07126 self adjusting connections and cartridges containing the same 3.0 x 150 IN5020-07125 high performance packing material 3.0 x 100 IN5020-07124 3.0 x 75 IN5020-07123 3.0 x 50 IN5020-07122 3.0 x 33 IN5020-07121 4.0 x 250 IN5020-07136 Inertsil CX Guard Columns 4.0 x 150 IN5020-07135 Dimensions 5 μm 5 μm Column 4.0 x 100 IN5020-07134 Replacement ID (mm) ID x Length (mm) (Guard Column E Set) 4.0 x 75 IN5020-07133 *Includes 1 holder Cartridges + 2 cartridges 4.0 x 50 IN5020-07132 1.0 x 10 IN5020-19284 IN5020-19234 1.0 4.0 x 33 IN5020-07131 1.5 x 10 IN5020-19384 IN5020-19334 1.5, 2.1 4.6 x 250 IN5020-07146 3.0 x 10 IN5020-19184 IN5020-19134 3.0 4.6 x 150 IN5020-07145 4.0 x 10 IN5020-19084 IN5020-19034 4.0, 4.6 4.6 x 100 IN5020-07144 3.0 x 20 IN5020-19584 IN5020-19534 2.1, 3.0 4.6 x 75 IN5020-07143 4.0 x 20 IN5020-19484 IN5020-19434 4.0, 4.6 4.6 x 50 IN5020-07142 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 4.6 x 33 IN5020-07141 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-55 LIFE SCIENCE
Colonnes HPLC/UHPLCColonnes HPLC/UHPLC GL SCIENCES HPLC COLUMN CATALOG Please contact your sales representative for a copy of the new GL Sciences catalogue. E-56 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE
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