CLS_MN_Catalogue

HPLC/UHPLC Columns • Totally spherical particle shape HPLC/UHPLC Columns Since 1911 • Outstanding surface microstructur • High pressure stability • Low metal content The NUCLEODUR® column family has expanded! NUCLEODUR® is a fully synthetical type B silica offering highly advanced physical properties: 18 Phases C18 Gravity | C18 Gravity-SB | C8 Gravity | C18 Isis | C18 Pyramid | PolarTec | Phenyl-Hexyl | π2 | PFP | Sphinx RP C18 HTec | C18 ec | C8 ec | C4 ec | HILIC | CN/CN-RP | NH2/NH2-RP | SiOH 1 Pore Size 110 Å 3 Standard Particle Sizes 1.8 μm | 3 μm | 5 μm | 7, 10, 12 and 16 μm particles for preparative purposes on request 7 Standard Column Lengths 30 mm | 50 mm | 75 mm | 100 mm | 125 mm | 150 mm | 250 mm 4 Standard Column IDs 2 mm | 3 mm | 4 mm | 4.6 mm NUCLEOSIL® 18 Phases C18 | C18 AB | C18 HD | C18 Nautilus | C18 Protect I | C8 ec | C8 | C8 HD | C4 | C2 | C6H5 | CN | OH | NH2 N(CH3)2 | SA | SB | SiOH 7 Pore Sizes 50 Å | 100 Å | 120 Å | 300 Å | 500 Å | 1000 Å | 4000 Å 4 Standard Particle Sizes 3 µm | 5 µm | 7 µm | 10 µm 4 Standard Column Lengths 250 mm | 150 mm | 125 mm | 100 mm 4 Standard Column IDs 2 mm | 3 mm | 4 mm | 4.6 mm 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN F-1 LIFE SCIENCE

Colonnes HPLC/UHPLC Overview of NUCLEODUR® HPLC Phases (Macherey-Nagel) Colonnes HPLC/UHPLC Phase Specification Particle Size Pore Size Surface Area Carbon Load Endcapped (µm) (Å) (m2/g) (%) C18 Gravity octadecyl phase, high density 1.8, 3, 5 110 340 18 Yes coating multi-endcapping. 18% C ∙ USP L1 C8 Gravity octyl phase, high density coat- 1.8, 3, 5 110 340 13 Yes ing multi-endcapping. 110 340 20 Yes 11% C ∙ USP L7 110 340 14 Yes C18 Isis octadecyl phase with specially 1.8, 3, 5 crosslinked surface modifica- tion endcapping 20% C ∙ USP L1 C18 Pyramid C18 modification with polar 1.8, 3, 5 endcapping 14% C ∙ USP L1 PolarTec octadecyl with embedded polar 1.8, 3, 5 110 340 17 Yes group. USP - L11 octadecyl phase, medium C18 ec density coating endcapping 3, 5 110 340 17.5 Yes 17.5% C ∙ USP L1 C8 ec octyl phase, medium density 3, 5 110 340 10.5 Yes coating endcapping 10.5% C ∙ USP L7 Contact your sales representative for more information F-2 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Overview of NUCLEODUR® HPLC Phases (Macherey-Nagel) Structure Application Similar Separation Principle - Retention Mechanism HPLC/UHPLC Columns Phases** NUCLEODUR® in general compounds with NUCLEOSIL® C18 HD (Si-O2)n ionizable functional groups such Waters Xterra® RP18 / MS C18; as basic pharmaceuticals and Phenomenex Luna C18 (2), pesticides Synergi and Max RP; Zorbax Extend C18; Inertsil ODS III; Purospher RP-18; Star RP-18 only hydrophobic Si(CH3)3 CH3 interactions H3C N NUCLEODUR® (Si-O2)n (van der Waals O interactions) like C18 Gravity, however NUCLEOSIL® C8 HD generally shorter retention times Waters Xterra® RP8 / MS C8; for nonpolar compounds Phenomenex Luna C8; Zorbax Eclipse; XDB-C8 NUCLEODUR® high steric selectivity, NUCLEOSIL® C18 AB steric interactions (Si-O2)n thus suited for separation Inertsil ODS-P; and hydrophobic of positional and structural YMC Pro C18RS isomers, planar/nonplanar interactions molecules NUCLEODUR® OH basic pharmaceutical Phenomenex Aqua; hydrophobic OH CH3 (Si-O2)n OH ingredients, very polar YMC AQ; interactions H3C N compounds, organic acids and polar Pol Waters Atlantis® dC18 interactions O Si OH (H bonds) NUCLEODUR® Pol Exceptional selectivity for NUCLEOSIL® C18 Nautilus Pol (Si-O2)n Si O Si(CH3)3 phenols and nitrogen containing ProntoSIL® C18; Zorbax® compounds, polar compounds Bonus-RP, Po-laris® Amide- Hydrophobic and Si(CH3)3 HO C18; Ascentis® RP Amide; polar (H bonds) like basic pharmaceuticals, SymmetryShield™ RP18; organic acids, pesticides, amino SUPELCOSIL™ LC-ABZ+; Pol acids, water soluble vitamins, HyPURITY™ ADVANCE etc. NUCLEODUR® Si OH robust C18 phase for routine NUCLEOSIL® C18 only hydrophobic Si(CH3)3 CH3 (Si-O2)n Si O Si(CH3)3 analyses Spherisorb® ODS II; Hypersil interactions SiOH H3C N ODS; Waters Symmetry® C18; NUCLEODUR® Si OH robust C8 phase for routine Inertsil ODS II; Kromasil C18; (van der O (Si-O2)n Si O Si(CH3)3 analyses Waals interactions) LiChrospher RP 18 some residual NUCLEOSIL® C8 ec / C8 silanol interactions Spherisorb® C8; Hypersil MOS; Waters Symmetry® C8; Kromasil C8; LiChrospher RP 8 ** phases which provide a similar selectivity based on chemical and physical properties Contact your sales representative for more information 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN F-3 LIFE SCIENCE

Colonnes HPLC/UHPLC Overview of NUCLEODUR® HPLC Phases (Macherey-Nagel) Colonnes HPLC/UHPLC Phase Specification Particle Size Pore Size Surface Area Carbon Load Endcapped (µm) (Å) (m2/g) (%) octadecyl, high density coating, high capacity, 3, 5 110 340 18 Yes multi-endcapping USP - L1 bifunctional RP phase, 1.8, 3, 5 110 340 15 Yes balanced ratio of propyl- phenyl and C18 ligands; endcapping 15% C; USP L1 and L11 pentafluorophenylpropyl, PFP multi-endcapping 1.8, 3, 5 110 340 8 Yes USP - L43 HILIC ammonium – sulfonic acid 1.8, 3, 5 110 340 7 No cyano (nitrile) phase for NP 3, 5 110 340 7 Yes and RP separations 7% C ∙ USP L10 aminopropyl for NP and NH2/NH2-RP RP separations 3, 5 110 340 2.5 Yes USP - L8 unmodified SiOH NUCLEODUR® 3, 5 110 340 - No USP - L3 Contact your sales representative for more information F-4 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Overview of NUCLEODUR® HPLC Phases (Macherey-Nagel) Structure Application Similar Separation Principle - Retention Mechanism HPLC/UHPLC Columns Phases** NUCLEODUR® Sophisticated analytical and XTerra® RP18 / MS C18 / only hydrophobic Si(CH3)3 CH3 (Si-O2)n preparative separations of basic, SunFire™ C18; Luna® C18(2), interactions H3C N neutral and acidic pharmaceu- Gemini®, Synergi® Max RP; ticals, derivatised amino acids, Zorbax® Extend-C18; Inertsil® (van der Waals O pesticides, fat-soluble vitamins, interactions) aldehydes, ketones and phenolic ODS III;Purospher® STAR RP-18; Hypersil™ BDS compounds NUCLEODUR® compounds with aromatic and no similar phases π-π interactions (Si-O2)n multiple bond systems and hydrophobic NO2 interactions NUCLEODUR® NUCLEODUR® (Si-O2)n Si OH Aromatic and unsaturated com- ACQUITY® CSH Fluoro- polar interactions O FF pounds, phenols, halogenated Phenyl; Hypersil™ GOLD PFP; (H bonds), F compounds, isomers, polar Luna® PFP(2); Discovery® HS dipole-dipole O FF compounds like pharmaceuti- F5; Allure® PFP Propyl, Ultra II interactions, cals, antibiotics; high retention of FF H Si O Si(CH3)3 PFP Propy π-π interactions, basic compounds hydrophobic F interactions FF CH3 SO3 – Hydrophilic compounds such H3C + SO3 – as organic polar acids and N (Si-O2)n +N bases, polar natural compounds, CH3 SO3 – nucleosides, oligonucleotides, O CH3 Si OH CH3 amino acids, peptides, water SeQuant™ ZIC®-HILIC; Ionic / hydrophilic CH3 Obelisc™ and electrostatic HO N NH soluble vitamins H3C + +N N SO3 – NH2 CH3 Si OH CH3 NUCLEODUR® CN polar organic compounds NUCLEOSIL® CN / CN-RP π-π interactions, C≡N O (Si-O2)n Si OH (basic drugs, molecules contain- polar interactions C ≡N HO CN ing π electron systems (H bonds), Si O Si(CH3)3 hydrophobic interactions NH2 NUCLEODUR® NUCLEODUR® (Si-O2)n Si OH polar compounds under RP NUCLEOSIL® NH2 / NH2-RP Polar / ionic and + NH3 conditions (sugars, DNA hydrophobic NH2 OH Si OH bases), hydrocarbons under NP O conditions (Si-O2)n Si OH polar and midpolar compounds Unmodified NUCLEOSIL® Polar / ionic SiOH O2N Si OH under normal phase conditions ** phases which provide a similar selectivity based on chemical and physical properties Contact your sales representative for more information 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN F-5 LIFE SCIENCE

Colonnes HPLC/UHPLC Nucleosil (Macherey-Nagel) Colonnes HPLC/UHPLC • Spherical porous silica • Wide range of bonded phases • Excellent mechanical stability NUCLEOSIL® 100 Å and 120 Å have very similar pore sizes and relative retention characteristics. NUCLEOSIL® 100 Å has the greater capacity while the 120 Å material exhibits the lower column back pressure. NUCLEOSIL® 300 Å silica is a wide pore, used as the base material for the reversed-phase bioanalytical columns. NUCLEOSIL® 100-5C18AB was the first of the newer generation NUCLEOSIL® materials which include the HD (high density) phases and the Nautilus, for use with up to 100% aqueous eluents. • Wider pore NUCLEOSIL® (500, 1000 and 4000 Å), Polygosil and columns for special applications eg PAH and hop constituents can be supplied on request. • Available in ChromCart® cartridge format, please enquire for part numbers and pricing. NUCLEOSIL Phase Functional Group Endcapped Particle Size (µm) Pore Size (Å) Carbon Load (%) C18 Octadecyl Yes 3, 5, 7, 10 50, 100, 120 15 C18 AB Octadecyl Yes 5 100 25 C18 HD Octadecyl Yes 3, 5 100 20 C18 Nautilus Octadecyl Yes 3, 5 100 16 Protect I Protective polar group Yes 3, 5 100 11 C8 ec Yes 5 9 Octyl No 50, 100 9 C8 Octyl Yes 3, 5, 7, 10 100, 120 13 C8 HD Octyl Yes 3, 5 2 Butyl No 5, 7 100 3.5 C4 Dimethyl No 7 120, 300 8 C2 Phenyl No 5, 7 - C6H5 Cyanopropyl Yes 100 4.5 CN/ CN-RP Nitrophenyl No 5, 7, 10 100, 120 5 NO2 Diol No 5, 10 100, 120 3.5 OH aminopropyl No 5, 7 4 NH2/ NH2 RP Dimethylamino No 3, 5, 7 100 4 N(CH3)2 Benzenesulphonic acid No 100 15 SA Quarternary ammonium No 5 100, 120 - SB 5, 10 100 Silica - 5, 10 100 5, 7, 10 100 50, 100 Contact your sales representative for more information F-6 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE