Hichrom catalogue

Customer Service About Canadian Life Science Au suject de Canadian Life Science Founded in 1997, Canadian Life Science (CLS) has steadily grown Fondée en 1997, Canadian Life Science (CLS) n’a cessé de croître to Canada’s leading supplier of LC/GC columns, chromatography pour devenir un chef de file canadien dans la vente de colonnes and dissolution accessories as well as 2d long-term sample HPLC / GC, d’accessoires de chromatographie et dissolution, storage tubes and racks for bio-banking and compound ainsi que dans la vente de tubes et systèmes d’entreposage long management. terme pour échantillons. For over 22 years, CLS has provided customers with unparalleled Depuis plus de 22 ans, CLS offre à ses clients un service et service, expertise and a wide selection of quality and cost- une expertise inégalés, en plus d’un large éventail de produits effective products that offer maximum flexibility to solve your économiques de qualité offrant une flexibilité maximale afin de analytical challenges. CLS is your foundation for what matters. résoudre vos problèmes analytique. CLS est votre pilier pour ce qui compte. Everyone is accessible to you Nous sommes très accessible We believe that our customers like our personal touch. Nous avons une approche personnelle et nous répondons à tous We take your calls personally, not a computer, not voicemail . les appels lorsque vous téléphonez au bureau chef. Nous n’avons We have dedicated inside customer service staff who care, and pas de boîte vocale. take care for specific areas. We have knowledgeable outside staff Notre personnel est dévoué au service à la clientèle et ce à based in most of the larger cities accross Canada. travers le Canada. Si vous désirez parler directement à notre We supply world class products Directrice de comptes au Québec, vous pouvez contacter Canadian Life Science distributes for top-quality manufacturers. Genevieve Lemieux au 514.428.8034. Our product offering has been chosen in a way that will give you Nous choisissons les meilleurs produits au monde the maximum flexibility solving your analytical challenges. En tant que distributeur nous avons l’avantage de choisir les Technical Support meilleurs fournisseurs et notre gamme complète de produit vous Our technical staff is happy to respond to customer enquiries. donne l’embarras du choix. We can also supply extensive application information from one Support Technique of the world’s largest data bases. Notre équipe technique se fait toujours un plaisir de vous aider à Plan the best delivery faire le meilleur choix pour vos applications. When you decide to purchase a product from Canadian Life Service et logistique Science our customer service department will find the fastest Lorsque vous placez une commande chez nous, nous nous and most economical way to send your order to you. For assurons de choisir le moyen le plus efficace et économique your convenience we have two warehouse locations, one in d’acheminer la marchandise. Edmonton, AB and one in Peterborough, ON. Nous avons deux entrepôts dont un en Alberta pour servir nos We’re glad to help clients de l’Ouest et un en Ontario pour servir nos clients de l’Est. We are never too busy to take your call. C’est un plaisir de vous servir Try us. You’ll notice the difference. Nous ne sommes jamais trop occupés pour vous répondre. Canadian Life Science, friendly people to do business with! Essayez-nous et vous verrez la différence. Il est agréable de faire affaire avec nous! I-22 CANADIAN Proud member of the Chrom4 buying group Fier membre du groupe dʼacheteur Chrom4 LIFE SCIENCE 1 888-226-2775 :: [email protected] :: www.lifescience.ca

NEW PRODUCTS New Products ACE® SuperC18TM UHPLC/HPLC Columns New Products • Exploit selectivity at low, intermediate and high pH • Use with LC-MS compatible buffers between pH 1.5 and 11.5 • Ultra-inert for maximum performance and reproducibility • 2, 3, 5 and 10μm phases ACE SuperC18 ultra-inert silica based UHPLC and HPLC columns were developed using unique Encapsulated Bonding Technology (EBTTM), which significantly increases ligand coverage of the silica surface and effectively eliminates the effect of unbonded silanol groups from separations. This results in improved inertness, chromatographic performance and stability. Changing pH is a powerful tool in enabling selectivity to be optimised and sample impurities to be identified. ACE SuperC18 columns provide excellent peak shape and lifetime under acidic, intermediate and high pH with LC-MS compatible buffers. In addition, they can be used with eluents containing methanol or acetonitrile, without suffering efficiency loss and/or extended equilibration times. Please contact Hichrom for further information. CHIRALPAX ZWIX(+) CHIRALPAX ZWIX(-) CHIRALPAK® ZWIXTM Chiral Phases Quinine-derivatized (8S,9R) Quinidine-derivatized (8R,9S) • High column efficiency due to 3μm particles Chiral selectors of CHIRALPAK ZWIX • Separation of underivatized amino acids and zwitterionic compounds • Control of enantiomer elution order with ZWIX(+) and ZWIX(-) CHIRALPAK ZWIX chiral stationary phases from Chiral Technologies can be used in both HPLC and SFC modes. These immobilized zwitterionic stationary phases incorporate both anion- and cation-exchange functional groups. The phases were developed specifically to exhibit stereoselectivity for zwitterionic molecules, such as underivatized amino acids and peptides. The chiral selectors separate zwitterionic molecules on the basis of a double ion-pairing mechanism. The use of polar eluents enables LC-MS to be used for detection and identification of many amino acids which lack chromophores. These zwitterionic ion exchangers complement the previously commercialised CHIRALPAK QD-AX and QN-AX anion-exchange columns (see page 85). New InertSustain® Phases In addition to the InertSustain C18 phase (see page 105), GL Sciences have introduced further phases in the InertSustain series. InertSustain C8, Phenyl and NH2 are based on the same newly developed ES (Evolved Surface) silica as the initial C18 phase. InertSustain NH2, with optimised aminopropyl bonding, is listed as an ultra performance sugar analysis column with separations based on weak ion-exchange and HILIC modes. It prevents the resolution of anomers at low temperatures, thereby offering easier sugar analyses. New YMC CHIRAL Polysaccharide Series Hichrom Limited • Polysaccharide chiral selectors 5 • Wide application range • High durability, also for SFC/SMB In addition to the existing YMC chiral phases (see page 274) YMC have introduced a new family of polysaccharide chiral columns. Based on the chiral selectors amylose tris (3,5-dimethylphenylcarbamate) and cellulose tris (3,5-dimethylphenylcarbamate), YMC CHIRAL Amylose-C and YMC CHIRAL Cellulose-C are coated phases offering a wide application range and high durability. They are supplied in 5, 10 and 20µm particle sizes. Columns and preparative bulk materials are available and additional immobilized phases are planned soon. www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660

New Products NEW PRODUCTS (continued) New Products New HALO®-5 Fused-Core® Phases Bridge the gap between • Fused-Core 5μm HALO-5 HILIC and HALO-5 Penta-HILIC HPLC and UHPLC • Higher efficiency and lower back pressure than fully porous 5μm phases • Well suited for use with legacy HPLC instruments Advanced Materials Technology have expanded the HALO-5 range of 5μm Fused-Core phases (described on page 128) to include HALO-5 HILIC and Penta-HILIC. These two phases contain the same bonding chemistries as their smaller particle size counterparts HALO HILIC and Penta-HILIC (see page 127). Both 5μm HALO-5 HPLC and the 2.7μm HALO UHPLC columns bridge the gap between HPLC and UHPLC. Kromasil® 2.5μm UHPLC/HPLC Columns • Based on same silica as larger particle classical 100Å Kromasil phases • C18, C8 and C4 bonding chemistries • Use in both HPLC and UHPLC systems Kromasil 2.5μm particle columns are based on the same stationary phases as other larger particle size Kromasil columns (see pages 141-147), enabling easy scaling from existing Kromasil methods to 2.5μm particle columns. These smaller particles are based on very narrow specification ranges, resulting in excellent resolution and column-to-column reproducibility. This also enables easy transfer of methods developed on 2.5μm particles to other departments, such as quality control. NUCLEODUR® and NUCLEOSHELL® Phenyl-Hexyl Phases Macherey-Nagel’s NUCLEODUR and NUCLEOSHELL Phenyl-Hexyl are the latest additions to the NUCLEODUR and NUCLEOSHELL ranges of HPLC/UHPLC columns discussed on pages 157-162. These phases offer alternative selectivity compared to classical C18 phases and are recommended for aromatic and unsaturated compounds and polar compounds. For free technical advice and support email [email protected] inorganic New Phases in YMC-Triart Series siloxane layer • Multi-layered hybrid material organic • Narrow particle and pore size distributions siloxane layer • 1.9, 3 and 5μm particle sizes YMC-Triart hybrid structure The innovative YMC-Triart series of phases, based on a multi-layer hybrid material, is discussed on pages 266-267. In addition to the C18, C8 and Diol-HILIC phases discussed on these pages, YMC-Triart Phenyl and YMC-Triart PFP are now also available. As for other phases in the YMC-Triart series, these 2 new phases are also available with particle sizes of 1.9, 3 and 5μm. Please contact Hichrom for ordering information for columns packed with these phases. 6

NEW PRODUCTS (continued) New Products Thermo Scientific GlycanPacTM AXH-1 Column New Products • Unique glycan selectivity based on charge, size and polarity • 1.9 and 3μm particle sizes • Designed for high resolution and high throughput analyses • Compatible with fluorescence and MS detection Thermo Scientific’s GlycanPac AXH-1 column is a high performance silica-based column for simultaneous separation of glycans by charge, size and polarity. The column is based on innovative mixed-mode surface chemistry, specifically designed for qualitative, structural analysis and characterisation of uncharged and charged glycans present on proteins. GlycanPac AXH-1 offers unique selectivity for biologically important labelled or native glycans by LC-fluorescence and LC-MS methods. Thermo Scientific AcclaimTM SEC Columns • Proprietary mono-dispersed multi-pore hydrophilic resin • Low column bleed, compatible with UV, RI, MS, ELSD and Corona CAD detectors Thermo Scientific Acclaim SEC-300 (5μm) and SEC-1000 (7μm) are a family of high performance SEC columns specifically designed for determining the molecular weight distribution and characterisation of water soluble polymers used in a wide range of products found in pharmaceuticals, cosmetics, food and industrial processes. Two columns with different pore sizes offer a wide linear calibration range of 100 to over 1,000,000 daltons. Columns are packed with small particle size, spherical, mono-dispersed hydrophilic polymethacrylate particles. CHROMABOND® HILIC SPE Columns and Sorbent • Zwitterionic silica phase for SPE of polar analytes CHROMABOND HILIC is an ammonium – sulphonic acid modified silica phase for SPE, based on the same chemistry as the NUCLEODUR HILIC phase (see page 158). This zwitterionic polar phase has a pore size of 60Å, particle size of 45μm and pH stability of 2 – 8. Recommended applications include polar organic acids and bases, polar natural compounds, nucleosides, oligonucleotides, amino acids, peptides and water soluble vitamins. The water-rich layer formed on the surface of the adsorbent enables stronger interactions for polar than for non-polar analytes. Thus polar analytes are more strongly retained than non-polar compounds, the inverse to reversed-phase materials such as CHROMABOND C18. CHROMABOND HILIC is available in polypropylene columns and as loose sorbent. ACE C18-Amide Hichrom Limited • Unique stationary phase 7 • Enhanced retention of polar compounds • Alternative selectivity to C18 columns ACE C18-Amide is a new, uniquely designed stationary phase offering enhanced retention of polar compounds and alternative selectivity to C18 based products. The unique ligand design of the ACE C18-Amide incorporates a longer alkyl chain spacer between the silica surface and amide polar group that gives extra phase stability in eluents of pH 2 - 8. The longer C18 tail increases the hydrophobic mechanism of interaction compared to phases with shorter (C14 - C16) tail groups. ACE C18-Amide is available in 2, 3, 5 and 10µm particle sizes. www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660

NEW PRODUCTS (continued) New Products New Products TOYOPEARL® MX-Trp-650M Resin • Multi-modal cation-exchange resin • High binding capacity for IgG and other proteins • Sharp elution peaks with mild conditions • Excellent pressure/flow characteristics TOYOPEARL MX-Trp-650M is a new multi-modal cation-exchange resin, with unique selectivity and high recovery. It provides high protein binding capacities and tolerates high conductivity feedstocks. In addition to ionic groups, its ligand also contains hydrophobic regions. Thus, the binding of target molecules is determined by electrostatic and hydrophobic contributions. TOYOPEARL MX-Trp-650M is particularly suited for the purification of target molecules that are difficult to purify using common purification platforms. YMC-Omega LC Media • Preparative media for polyunsaturated fatty acids (PUFAs) • Enhanced selectivity of structural isomers of PUFAs • Available in 10μm particle size The demand for highly enriched fatty acid fractions is rising. Industrial scale HPLC is the most suitable method for the production of high purity PUFA fractions from fish oils, for the pharmaceutical and dietary supplement industries. This new preparative media from YMC is a silica-based stationary phase specifically designed for industrial scale chromatography for the separation of PUFAs, such as Omega-3 and Omega-6 fatty acids. The phase has proprietary bonding and optimised pore size. It enables high loadability and high recovery of fatty acids. For free technical advice and support email [email protected] TraceGOLDTM Fast GC Columns • Faster analysis with the same separation • Easy optimisation of conventional methods TraceGOLD Fast GC columns are shorter and have smaller i.d.s than conventional GC columns. By selecting the most appropriate TraceGOLD Fast GC column, column performance and separation can be maintained while decreasing analysis time. For a given stationary phase, if the phase ratio is kept constant, then the selectivity can be maintained, leading to increased efficiency. This allows a similar separation to be performed with a shorter column length. Phase ratio (β) = column i.d. (μm) / 4 x film thickness (μm) For instance, the performance of a 30m x 0.25mm x 0.25μm column can be achieved with a 20m x 0.15mm x 0.15μm column in up to 30% less time. Please see pages 289-290 for details of conventional dimension TraceGOLD GC columns. New Silica Based TSKgel Columns for Aqueous SEC/Gel Filtration • Three new (U)HPLC columns for antibody analysis • TSKgel SuperSW mAb HR • TSKgel SuperSW mAb HTP • TSKgel UltraSW Aggregate Tosoh Bioscience have introduced three new silica based (U)HPLC TSKgel columns for aqueous SEC/gel filtration, each designed to address a different aspect of antibody analysis. Both TSKgel mAb HR and HTP have a particle size of 4µm and a pore size of 250Å. TSKgel mAb HR offers the best separation of antibody monomers and dimers, whereas TSKgel mAb HTP is UHPLC compatible, suitable for fast separation of antibody monomers and dimers. TSKgel UltraSW Aggregate (3µm, 300Å) is designed for higher resolution analysis of a wider range of high molecular weight aggregates. Guard columns for all three columns are available. 8