CHROM4_Catalogue 2019_Web2

Sample Preparation iris n Features & Benefits • Localized Hydrophilic/Lipophilic Base (HLB type sorbent) – using a combination of polar and non-polar interactions to provide optimal analyte/resin contact • Polyamide Stationary Phase – excellent water wettability using reversed-phase mode up to 100% aqueous solvent or normal-phase mode using up to 100% organic solvents IRIS N APPLICATION IRIS N - 30mg/1mL Estriol, Hydrocortisone, IR44461 Betamethasone Sorbent: 6 µg/mL in H2O Part Number: Solution Conc: Estriol Hydrocortisone Betamethasone Run 1 109.5% Recovery Rates: Run 2 104.1% Run 1 104.4% Run 1 100.3% Run 3 109.3% Run 2 102.6% Run 2 100.8% Run 3 103.8% Run 3 100.0% Hydrocortisone Estriol 14 12 mAU 10 Estriol Hydrocortisone Beamethasone 8 6 4 2 Betamethasone 0 O OH 3 5 7 9 11 13 15 17 19 HO OH Time (min) H FH O [email protected] :: www.chrom4.com 49

Sample Preparation MCX iris mcx • Sulfonated DVB phase • Pore size: 80 Å • Ion-Exchange capacity: ~1 meq/g • pKa <1 • Available in 25-35 µm particle size • Available in various cartridge sizes and 96-well plate formats IRIS MCX is a water-wettable sulfonated divinylbenzene mixed-mode phase for both cation-exchange and reversed-phase applications. IRIS MCX allows for easy method development, through its dual retention qualities, for a wide range of basic and neutral compounds. IRIS MCX shows excellent retention for essentially all of the common basic drugs of abuse and similar amine containing compounds. The phase is stable over the complete pH range (0-14) and can be used with nearly any solvent, allowing the use of a wide range of solvents. IRIS MCX SPE Cartridges Part No. Description Particle Size Qty (Box) IR54460 IRIS MCX 10mg/1mL 25-35µm 100 IR54461 IRIS MCX 30mg/1mL 25-35µm 100 IR54462 IRIS MCX 30mg/3mL 25-35µm 100 IR54446 IRIS MCX 60mg/3mL 25-35µm 100 IR54264 IRIS MCX, 100mg/3mL 25-35µm 100 IR54445 IRIS MCX 200mg/6mL 25-35µm 30 IR54266 IRIS MCX 500mg/6mL 25-35µm 30 IRIS MCX SPE 96-well Plates Part No. Description Particle Size Qty (Box) IR96541 IRIS MCX 96-well 10mg/1mL 25-35µm 1 IR96543 IRIS MCX 96-well 30mg/1mL 25-35µm 1 IR96545 IRIS MCX 96-well 60mg/1mL 25-35µm 1 IR96547 IRIS MCX 96-well 30mg/2mL 25-35µm 1 IR96548 IRIS MCX 96-well 60mg/2mL 25-35µm 1 50 [email protected] :: www.chrom4.com

Sample Preparation iris mcx Features & Benefits • Sulfonated DVB Phase – allows for cation-exchange and reversed-phase separations • 100% DVB – no swelling, increased retention, excellent flow properties and pH stability • Excellent Wettability – even after drying, due to hydrophilic/hydrophobic balance IRIS MCX APPLICATION Norephedrine & Ephedrine Sorbent: IRIS MCX - 30 mg/1mL Hydrochloride Part Number: IR54461 Solution Conc: 50 µg/mL in H2O 0.1% Formic Acid Recovery Rates: Norephedrine Ephedrine Hydrochloride Hydrochloride Run 1 94.2% Run 1 94.7% Run 2 91.8% Run 2 92.7% Run 3 93.4% Run 3 96.4% Note 1: After wash step cartridge was dried for 1 minute using vac. The collected elute solution was placed in a 2 ml volumetric and brought to the mark with H2O (0.1% formic acid). Norephedrine Hydrochloride 23 18 Ephedrine Hydrochloride 13 mAU Norephedrine Hydrochloride Ephedrine Hydrochloride 8 3 -2 4 6 8 10 12 14 Time (min) [email protected] :: www.chrom4.com 51

Sample Preparation WCX iris wcx • Organic acid on DVB phase • Pore size: 80 Å • Ion-Exchange capacity: ~1 meq/g • pKa ~5 • Available in 25-35 µm particle size • Available in various cartridge sizes and 96-well plate formats IRIS WCX is a weak cation-exchange divinylbenzene polymer phase. IRIS WCX eliminates the guesswork commonly found in lengthy method development associated with strong bases such as quaternary amines. This phase utilizes the chemical functionality of a carboxylic acid. When the phase is negatively charged, it retains strong bases such as quaternary amines and releases them when neutral. IRIS WCX has repeatedly proven consistency and reliability, reducing the need for time consuming and costly retesting. IRIS WCX SPE Cartridges Part No. Description Particle Size Qty (Box) IR84012 IRIS WCX 10mg/1mL 25-35µm 100 IR84013 IRIS WCX 30mg/1mL 25-35µm 100 IR84023 IRIS WCX 30mg/3mL 25-35µm 100 IR84016 IRIS WCX 60mg/3mL 25-35µm 100 IR84019 IRIS WCX 200mg/6mL 25-35µm 30 IR84020 IRIS WCX 500mg/6mL 25-35µm 30 IR84021 IRIS WCX 1gram/6mL 25-35µm 10 IRIS WCX SPE 96-well Plates Part No. Description Particle Size Qty (Box) IR97008 IRIS WCX 96-well 10mg/1mL 25-35µm 1 IR97010 IRIS WCX 96-well 30mg/1mL 25-35µm 1 IR97012 IRIS WCX 96-well 60mg/1mL 25-35µm 1 IR97014 IRIS WCX 96-well 30mg/2mL 25-35µm 1 IR97015 IRIS WCX 96-well 60mg/2mL 25-35µm 1 52 [email protected] :: www.chrom4.com

Sample Preparation iris wcx Features & Benefits • Organic Acid Phase – allows for tunable selectivity for strong bases • 100% DVB – no swelling, increased retention, excellent flow properties and pH stability • Retain and Release – easy separation of strong bases • Uniquely Selective – fast and efficient sample preparation and purification for strong bases IRIS WCX APPLICATION Sorbent: IRIS WCX - 30 mg/1mL Sulfonamides Part Number: IR84013 Solution Conc: 5 µg/mL in H2O Recovery Rates: Sulfadiazine Sulfathiazole Sulfamerazine Sulfamethazine Run 1 98.8% Run 1 95.0% Run 1 99.9% Run 1 99.5% Run 2 102.2% Run 2 98.1% Run 2 100.5% Run 2 99.4% Run 3 98.2% Run 3 93.8% Run 3 95.6% Run 3 96.5% Sulfadiazine Sulfamerazine Sulfathiazole Sulfathiazole 20 18 Sulfamerazine 16 Sulfadiazine 14 mAU 12 Sulfamethazine 10 8 6 4 Sulfamethazine 2 0 O H N 3 5 7 9 11 13 15 S N Time (min) ON H2N [email protected] :: www.chrom4.com 53

Sample Preparation MAX iris max • Quaternary amine on DVB phase • Pore size: 80 Å • Ion-Exchange capacity: ~0.1 meq/g • pKa >18 • Available in 25-35 µm particle size • Available in various cartridge sizes and 96-well plate formats IRIS MAX is a water-wettable quaternary amine divinylbenzene polymer phase designed for both anion-exchange and reversed-phase extraction. As a result of its bifunctionality, IRIS MAX produces outstanding retention for charged and neutral compounds. It is excellent for compounds containing an organic acid or similar ionizable functional groups. The phase is stable over the complete pH range (0-14) and can be used with nearly any solvent permitting the use of a wide range of solvents. IRIS MAX SPE Cartridges Part No. Description Particle Size Qty (Box) IR64460 IRIS MAX 10mg/1mL 25-35µm 100 IR64461 IRIS MAX 30mg/1mL 25-35µm 100 IR64462 IRIS MAX 30mg/3mL 25-35µm 100 IR64446 IRIS MAX 60mg/3mL 25-35µm 100 IR64264 IRIS MAX, 100mg/3mL 25-35µm 100 IR64445 IRIS MAX 200mg/6mL 25-35µm 30 IR64266 IRIS MAX 500mg/6mL 25-35µm 30 IRIS MAX SPE 96-well Plates Part No. Description Particle Size Qty (Box) IR96641 IRIS MAX 96-well 10mg/1mL 25-35µm 1 IR96643 IRIS MAX 96-well 30mg/1mL 25-35µm 1 IR96645 IRIS MAX 96-well 60mg/1mL 25-35µm 1 IR96647 IRIS MAX 96-well 30mg/2mL 25-35µm 1 IR96648 IRIS MAX 96-well 60mg/2mL 25-35µm 1 54 [email protected] :: www.chrom4.com

Sample Preparation iris max Features & Benefits • Quaternary Amine Phase – allows for anion-exchange and reversed-phase separations • 100% DVB – no swelling, increased retention, excellent flow properties and pH stability • Excellent Wettability – even after drying, due to hydrophilic/hydrophobic balance IRIS MAX APPLICATION Sorbent: IRIS MAX - 30 mg/1mL DL-Tyrosine Part Number: IR64461 Solution Conc: 40 µg/mL in H2O Recovery Rates: Run 1 92.9% 90.8% Run 2 93.1% Run 3 12 10 DL-Tyrosine 8 O mAU 6 O 4 2 OH 0 NH2 0 2 4 6 8 10 12 14 Time (min) [email protected] :: www.chrom4.com 55

Sample Preparation WAX iris wax • Secondary amine on DVB phase • Pore size: 80 Å • Ion-Exchange capacity: ~1 meq/g • pKa ~7 • Available in 25-35 µm particle size • Available in various cartridge sizes and 96-well plate formats IRIS WAX is a mixed-mode weak anion-exchange divinylbenzene polymer phase, designed to retain and release strong acids such as sulfates. Depending on the requirements of the application, this phase can be used in the neutral or positively charged state. IRIS WAX displays both anion-exchange and reversed-phase behavior, which promotes excellent retention over a wide range of hydrophilicity for both acidic, when phase is positively charged, and neutral compounds. IRIS WAX SPE Cartridges Part No. Description Particle Size Qty (Box) IR86011 IRIS WAX 10mg/1mL 25-35µm 100 IR86012 IRIS WAX 30mg/1mL 25-35µm 100 IR97516 IRIS WAX 30mg/3mL 25-35µm 100 IR86015 IRIS WAX 60mg/3mL 25-35µm 100 IR86018 IRIS WAX 200mg/6mL 25-35µm 30 IR86019 IRIS WAX 500mg/6mL 25-35µm 30 IRIS WAX SPE 96-well Plates Part No. Description Particle Size Qty (Box) IR97508 IRIS WAX 96-well 10mg/1mL 25-35µm 1 IR97510 IRIS WAX 96-well 30mg/1mL 25-35µm 1 IR97512 IRIS WAX 96-well 60mg/1mL 25-35µm 1 IR97514 IRIS WAX 96-well 30mg/2mL 25-35µm 1 IR97515 IRIS WAX 96-well 60mg/2mL 25-35µm 1 56 [email protected] :: www.chrom4.com

Sample Preparation iris wax Features & Benefits • Secondary Amine Phase – allows for tunable selectivity for strong acids • 100% DVB – no swelling, increased retention, excellent flow properties and pH stability • Excellent Retention – bifunctional with reversed-phase and anion-exchange behavior • Uniquely Selective – fast and efficient sample preparation and purification for strong acids IRIS WAX APPLICATION Sorbent: IRIS WAX - 30 mg/1mL Nitro-L-Tyrosine, Iodo-L-Tyrosine, Part Number: IR86012 N-Acetyl-L-Tyrosine Solution Conc: 10 µg/mL in H2O Recovery Rates: Nitro-L-Tryosine Iodo-L-Tyrosine N-Acetyl-L-Tyrosine Run 1 99.1% Run 1 96.7% Run 1 94.8% Run 2 102.6% Run 3 103.6% Run 2 98.0% Run 2 98.8% Run 3 98.2% Run 3 95.0% Nitro-L-Tyrosine Iodo-L-Tyrosine O2N O HO HO H NH2 OH NH2 24 I OH Nitro-L-Tyrosine O 19 mAU 14 Iodo-L-Tyrosine 9 N-Acetyl-L-Tyrosine 4 N-Acetyl-L-Tyrosine -1 10 15 20 25 30 O 5 Time (min) OH HO HN O [email protected] :: www.chrom4.com 57

Sample Preparation Applications - Environmental Sorbent: IRIS PLUS - 200 mg/6mL Polycyclic aromatic Part Number: IR34445 hydrocarbons - PAH Solution Conc: 10 µg/mL in H2O Recovery Rates: Chrysene Benzo(b)Fluoranthene Benzo(a) Benzo(j)Fluoranthene Anthracene Benzo(k)Fluoranthene 104.8% 106.5% Benzo(a)pyrene Indeno(1,2,3-cd)pyrene Group 2 Group 4 Benzo(e)pyrene Benzo(g,h,i)perylene Group 1 Group 3 Dibenz(a,h)anthracene 106.5% 98.7% Figure 1: GCMS Chromatogram Figure 1: GCMS Chromatogram Sorbent: IRIS N - 30 mg/1mL Carbaryl, Atrazine, Part Number: IR44461 Methiocarb, Alachlor Solution Conc: 1, 1, 5, 10 µg/mL in H2O Recovery Rates: Carbaryl Atrazine Methiocarb Alachlor Run 1 102.0% Run 1 98.0% Run 1 101.4% Run 1 98.0% Run 2 100.1% Run 2 101.1% Run 2 101.0% Run 2 101.1% Run 3 99.9% Run 3 102.2% Run 3 99.6% Run 3 102.2% Carbaryl O Methiocarb O NH 39 Carbaryl H O 34 N 29 Methiocarb 24 O O 19 Atrazine S mAU 14 9 CH3 Atrazine 4 Alachlor Alachlor O CI N CI H O CH3 N CH2 CH3 N NN -1 3 4 5 65 8 8 9 10 11 12 H3C H N CH CH3 CH3 58 [email protected] :: www.chrom4.com

Sample Preparation Applications - Environmental Sorbent: IRIS WCX - 30 mg/1mL Diquat Dibromide Part Number: IR84013 Solution Conc: 10 µg/mL in H2O Recovery Rates: Run 1 92.4% Run 2 92.7% Run 3 85.8% Note: The peak area values for Diquat were found to change over time. There was a relatively quick change to lower area values that then somewhat leveled off. The area values used for quantitation were for injections of the standards and the SPE solutions in this semi-stable time frame. This observed change in peak area over time is consistent with references that state the need to deactivate glassware prior to Diquat solution contact. In this analysis the SPE Diquat solution comes in contact with three different glass vessels. Diquat is not retained by the chromatographic column, eluting in the void volume. However, the strong absorbance at 310nm allows for quantitation without interference. 50 45 40 mAU 35 Diquat 30 Dibromide 25 20 15 1 2 3 4 56 N+ Time (min) Br 10 N+ 5 Br 0 0 Sorbent: IRIS MCX - 30 mg/1mL 2,3 Dihydroxynaphthalene Part Number: Solution Conc: IR54461 Recovery Rates: 10 µg/mL in H2O Run 1 109.5% Run 2 103.0% Run 3 101.6% 120 100 mAU 80 2,3 Dihydroxynaphthalene 40 OH OH 0 45 67 8 9 10 11 12 3 Time (min) [email protected] :: www.chrom4.com 59

Sample Preparation Applications - Food & Agriculture Sorbent: IRIS MCX - 200 mg/6mL Melamine Part Number: IR54445 Solution Conc: 1 mg/mL in H2O Internal Standard Recovery Rates: Melamine 97% Melamine Sorbent: IRIS N - 30 mg/1mL Florfenicol Thiamphenicol, Florfenicol, Part Number: IR44461 Run 1 99.1% Chloramphenicol Solution Conc: 5 µg/mL in H2O Run 2 101.1% Run 3 100.4% Chloramphenicol Recovery Rates: Thiamphenicol Run 1 99.3% Run 1 98.4% Run 2 100.3% Run 2 99.7% Run 3 99.8% Run 3 99.2% Note 1: Elution from the IRIS N SPE cartridge was performed with 70% CH3CN. While 100% CH3CN appeared to fully elute the compounds, the chromatographic peak shape was significantly better when the SPE elution solvent was less than 100% CH3CN. 70% was used because it was already prepared, lower CH3CN may lead to more improvement in chromatographic peak shape. Florfenicol 35 Thiamphenicol 30 Thiamphenicol 25 OH H CI 20 HO N mAU Florfenicol Chloramphenicol CI O O 15 S O Chloramphenicol 10 OH OH 5 C 0O HN C 3 4 5 6 7 8 9 10 11 12 N O Time (min) O 60 [email protected] :: www.chrom4.com

Sample Preparation Applications - Food & Agriculture Sorbent: IRIS MCX - 30 mg/1mL Carbendazim, Thiabendazole Part Number: IR54461 Solution Conc: Carbendazim 5 µg/mL in H2O Recovery Rates: Thiabendazole Carbendazim Thiabendazole Run 1 101.7% Run 1 99.1% Run 2 102.0% Run 2 100.1% Run 3 100.2% Run 3 98.0% 34 29 Thiabendazole 24 mAU 19 Carbendazim 14 9 4 -1 0 24 6 8 10 12 Time (min) Sorbent: IRIS WCX - 30 mg/1mL Sulfonamides Part Number: IR84013 Solution Conc: 5 µg/mL in H2O Sulfamerazine Sulfamethazine Run 1 99.9% Recovery Rates: Sulfadiazine Sulfathiazole Run 2 100.5% Run 1 99.5% Run 1 98.8% Run 1 95.0% Run 3 95.6% Run 2 102.2% Run 2 98.1% Run 2 99.4% Run 3 98.2% Run 3 93.8% Run 3 96.5% Sulfadiazine Sulfamerazine Sulfathiazole 20 18 Sulfamerazine 16 Sulfadiazine 14 mAU 12 Sulfamethazine 10 8 6 Sulfathiazole 4 Sulfamethazine 2 H N 0 O N 3 5 7 9 11 13 15 S ON Time (min) H2N [email protected] :: www.chrom4.com 61

Sample Preparation Applications - Clinical & Bioanalysis Sorbent: IRIS MCX 200 mg/6mL Opiates Part Number: IR54445 Codeine Solution Conc: 5 µg/mL in H2O Hydrocodone Recovery Rates: Hydrocodone 95% Dihydrocodeine 91% Methylmorphine (Codeine) 94% Oxycodone 104% Hydrocodone ISTD Hydromorphone Dihydrocodeine 6-acetylmophine Hydromorphone 93% Oxymorphone Morphine 87% Morphine Oxymorphone 94% ON Morphine ISTD Oxycodone OH H3CO O N O H CH3 Hydrocodone HO Methylmorphine (Codeine) O HO HO N H OH N N N O O O HO O HO H OH HO O O CH3 Dihydrocodeine Morphine Oxym orphone 6-a cetylm orphine Sorbent: IRIS N - 30 mg/1mL Antipsychotic Drugs Part Number: IR44461 Solution Conc: 25 µg/mL in H2O Recovery Rates: Risperidone 100% Quitiapine Fumerate 103% Domperidone 103% Risperidone Domperidone Quetiapine Fumarate 62 [email protected] :: www.chrom4.com

Sample Preparation Applications - Clinical & Bioanalysis Sorbent: IRIS N - 30 mg/1mL Prednisone, Methylprednisolone, Part Number: IR44461 Dexamethasone Solution Conc: Recovery Rates: 6 µg/mL in H2O Prednisone Methylprednisolone Dexamethasone Run 1 100.7% Prednisone Run 2 99.5% Run 1 97.7% Run 1 100.0% Run 3 99.8% O Run 2 98.3% Run 2 98.0% HO Run 3 99.9% Run 3 99.3% H HH Note: Elution of the hormones from the IRIS N SPE cartridge was performed with O 60% CH3CN. It was found that lower recoveries were obtained when using 100% CH3CN. Methylprednisolone CH20H 14 HO CH3 CO CH3 OH H O 12 H H O Prednisone OH 10 O mAU 8 Methylprednisolone Dexamethasone CH3 6 4 Dexamethasone 2 H3C HO O 0 H OH 3 5 7 9 11 13 15 HO H Time (min) 17 19 H3C H CH3 FH O Sorbent: IRIS N - 30 mg/1mL Warfarin, Carbamazapine, Diphenhydramine Part Number: IR44461 Solution Conc: Warfarin (2 µg/mL), Carbamazapine (1 µg/mL), Diphenhydramine (5 µg/mL) in H2O Recovery Rates: Warfarin Carbamazapine Diphenhydramine Carbamazapine Run 1 93.8% Run 2 95.8% Run 1 97.5% Run 1 100.4% Run 3 94.2% Run 2 98.0% Run 2 104.1% Run 3 99.2% Run 3 106.1% 100 90 N Warfarin 80 O NH2 O 70 Carbamazapine Diphenhydramine 60 CH3 O N O mAU 50 O CH3 HO 40 Warfarin Diphenhydramine 30 20 10 0 6 7 8 2 3 4 5 Time (min) [email protected] :: www.chrom4.com 63

LC Columns PARTISPHERE® CARTRIDGE SYSTEM • Void-sealing cartridge system PartiSphere® silica is manufactured by Hichrom Ltd. • Convenient and easy to use The material is only readily available in the PartiSphere • Hand tightened fittings cartridge system. PartiSphere Functional Group Particle Particle Size Pore Size Surface Area Carbon Load Phases shape (µm) (Å) (m2/g) (%) Octyl 120 C8 Octadecyl Spherical 5 120 160 6 C18 Quaternary Ammonium Spherical 5 120 160 11 SAX Benezenesulphonic Acid Spherical 5 120 160 - SCX Spherical 5 160 - Further PartiSphere® materials are available. Void-sealing cartridge system PartiSphere guard cartridge system The system is characterized by a void-sealing mechanism. If a Additional installation of the guard cartridge holder void eventually occurs at the top of the column bed, a simple (WH4631-0003) allows use of guard cartridges (Figure 1). tightening of the locking head will move the cone-sealing cylinder and frit downwards until it creates the zero-void configuration of Figure 1. PartiSphere cartridge system the original cartridge. Large knurled end fittings allow ready hand-tightening of the system. Guard cartridge holder (WH4631-0003) End fittings (WH4631-0001) Ordering Information - Cartridges Guard cartridges Analytical cartridges PartiSphere Phase Replacement guard cartridges (5/pk) PartiSphere Phase Catalogue No. Reversed-phase Catalogue No. Anion exchange 125 x 4.6mm replacement cartridge (1/pk) Cation exchange WH4641-0002 Accessories for guard cartridges WH4641-0005 C8 WH4621-0503 Guard Cartridge Holder (1/pk) WH4641-0007 C18 WH4621-0502 WH4631-1003 SAX WH4621-0505 SCX WH4621-0507 250 x 4.6mm replacement cartridge (1/pk) C18 WH4621-1502 SAX WH4621-1505 SCX WH4621-1507 Accessories Spare end fitting WH4631-1001 64 [email protected] :: www.chrom4.com

ISO 9001:2000 LC Columns ISO 14001:2004 PARTISIL® • Irregular porous silica • 5 and 10µm particle sizes Partisil®, manufactured by Hichrom Ltd., was one of the first • Hichrom high efficiency commercially available irregular silicas. A large surface area gives it a high loading capacity. Partisil® ion-exchange materials are still widely used today. Partisil® Functional Group Endcapped Particle Size Pore Size Surface Area Carbon Load Phases (µm) (Å) (m2/g) (%) Octyl - 85 Silica Octadecyl Yes 5,10 85 350 - C8 Octadecyl No 5,10 85 350 9 ODS Octadecyl Yes 10 85 350 5 ODS2 Octadecyl Yes 10 85 350 15 ODS3 Amino-cyano Np 5,10 85 350 10.5 PAC Tetramethyl Ammonium No 5,10 85 350 16 SAX Sulphonic Acid No 10 85 350 10 SCX 10 350 10 Ordering Information - Partisil® Columns Microbore (1.0mm - 2.1mm ID) and analytical (4.0mm ID) columns Please contact us for further details of 1.0mm, 2.1mm and 4.0mm ID Partisil columns as well as Partisil original part numbers. Medium bore (3.2mm ID) and analytical (4.6mm ID) columns Partisil® Phase Column Dimensions (mm) Guard Cartridges1 5µm 50 x 3.2 150 x 3.2 250 x 3.2 50 x 4.6 150 x 4.6 250 x 4.6 (For 3.2mm-4.0mm i.d. columns) Silica P5-50AS P5-150AS P5-250AS P5-50A P5-150A P5-250A P5C8-150A P5C8-250A P5-10C5 C8 P5C8-50AS P5C8-150AS P5C8-250AS P5C8-50A P5ODS3-150A P5ODS3-250A P5PAC-150A P5PAC-250A P5C8-10C5 ODS P5ODS3-50AS P5ODS3-150AS P5ODS3-250AS P5ODS3-50A P5ODS3-10C5 PAC P5PAC-50AS P5PAC-150AS P5PAC-250AS P5PAC-50A P5PAC-10C5 Partisil® Phase Column Dimensions (mm) Guard Cartridges1 10µm 250 x 4.6 Silica (For 3.2mm-4.0mm i.d. columns) C8 P10-250A ODS P10C8-250A P10-10C5 ODS2 P10ODS-250A P10C8-10C5 ODS3 P10ODS2-250A P10ODS-10C5 PAC P10ODS3-250A P10ODS2-10C5 SAX P10PAC-250A P10ODS3-10C5 SCX P10SAX-250A P10PAC-10C5 P10SCX-250A P10SAX-10C5 P10SCX-10C5 1 U se with free-standing holder HI-161 and column coupler HI-081. 2 Starter kits also available. Please contact us for ordering information on column dimensions not listed [email protected] :: www.chrom4.com 65

LC Columns ULTRASPHERE® (Hichrom) ISO 9001:2000 (Formerly Beckman) ISO 14001:2004 • Spherical porous silica Ultrasphere®, by Hichrom, is a classical silica which boasts a • 3 and 5µm particle sizes high performance reputation. In addition to the base silica, four • Specific ion-pair reversed-phase column phases (5μm particle size) are available in standard columns - • Widely referenced silica Ultrasphere C8, C18, cyano and IP (ion-pair). Ultrasphere C8 and C18 are also available as 3μm particle size silicas packed into the XL High Speed Cartridge Columns. Ultrasphere Endcapped Particle Size Pore Size Phases (µm) (Å) - 80 Silica Yes 5 80 C8 Yes 3,5 80 ODS No 3,5 80 CN Yes 5 80 IP (C18) 5 Batch to batch reproducibility Figure 1. Carbon load (%) for batches of Ultrasphere ODS The reproducibility of batches of Ultrasphere ODS is shown in Figure 1. Resultant columns have minimal selectivity differences (<5%). Ultrasphere-XL high speed cartridge column In this cartridge design, the guard cartridge is directly abutted to the analytical cartridge. This integral design was one of the first of its type to minimize dead volume between the two cartridges. Ordering Information XL Cartridge Column Column Dimensions2 (mm) Ultrasphere Dimensions1 (mm) Phases Analytical Guard (2pk) Analytical Guard Silica 250 x 4.6 45 x 4.6 C8 70 x 4.6 5 x 4.6 150 x 4.6 US235341 250 x 2.0 US244011 ODS US235342 US235332 US237392 US243532 CN -- US235333 US235329 US237396 US243533 C18 Ion-Pair (IP) US235330 US244071 US244434 US244072 US237501 US237521 US244070 US235335 US237394 US243534 US235334 US237500 US237520 - -- -- 1 Use analytical cartridge and guard cartridge in XL holder assembly US237060. Contains 3µm particle size silica. 2 Contains 5µm particle size silica. 66 [email protected] :: www.chrom4.com

LC Columns ReproSil®- Pur Basic & ReproSil® Gold (Maisch) • Porous spherical silica Comparison - C18 Phases • Specifically designed for pharmaceutical and biotechnical separations • Fully validated columns • Ideal for LC-MS • Excellent reproducibility Reprosil®-Pur Basic-C18 1) Octadecyl monolayer phase bonded to spherical silica p4a5r0tmicl2e/sg with pore size of 100Å, a surface area of and a pore volume of 1.1 ml/g. 2) Impurities: A l < 1ppm, Fe < 1ppm, Ti < 1ppm, Zr < 1ppm 3) Double bound endcapping. 4) pH stability from 1.5 to 10, because of the unique stable endcapping. 5) Excellent peak symmetry even with strong bases High Speed HPLC- Reprosil® Gold Features of Reprosil®-Pur and Reprosil® Gold - the high purity phases (99.999%) ReproSil Particle Size Pore Size Surface Area Carbon Load Endcapping Phases (µm) (Å) (m2/g) (%) 100 Bidentate Reprosil-Pur Basic C18 1.8, 2, 3, 5, 10 100 450 17 Bidentate Reprosil-Pur Basic C18-HD 3, 5, 10 100 450 25 Bidentate Reprosil-Pur Basic C8 3, 5, 10 100 450 15 Bidentate Reprosil-Pur Basic C8-2 3, 5, 10 450 12 Reprosil Gold C18 3, 5, 10 120 320 20 Bidentate Reprosil Gold 200 C18 5, 10 Reprosil Gold 300 C18 5, 10 200 180 14 Bidentate 300 120 8 Bidentate Reprosil Gold C8 3, 5, 10 120 320 12 Bidentate Reprosil Gold 200 C8 5, 10 Reprosil Gold 300 C8 5, 10 200 180 8 Bidentate 300 120 5 Bidentate Reprosil Gold C4 3, 5, 10 120 320 8 Bidentate Reprosil Gold 200 C4 5, 10 200 180 5 Bidentate Reprosil Gold 300 C4 5, 10 300 120 3 Bidentate Please contact us for ordering information [email protected] :: www.chrom4.com 67

LC Accessories REPLACEMENT PARTS - AGILENT™ Compatible Part 1200/1100/1050 Ordering Information - Pump Replacement Parts Pump Replacement Parts Replacement OEM Part Typical Maintenance PTFE Frits for model 1200/1100/1050 5/pack Part Number 01018-22707 Schedule Piston Seal, UHMW-PE for model 1200/1100/1050 - pk 10 CHR07-000-5 0905-1420 Change every 6 months Piston Seal, UHMW-PE for model 1200/1100/1050 - each CHR01-000-10 0905-1420 Change every 6 months Piston Seal, Black PTFE for model 1200/1100/1050 - pk 10 CHR01-000 5063-6589 Change every 6 months Piston Seal, Black PTFE for model 1200/1100/1050 - each CHR01-200-10 5063-6589 Change every 6 months UHMW PE Plunger Wash Seal for models 1200/1100/1050 CHR01-200 n/a Change every 6 months Black PTFE Based Plunger Wash Seal for models 1200/1100 CHR01-002-10 n/a Change every 6 months Replacement Piston for model 1200/1100/1050 CHR01-202-10 5063-6586 Change every 6 months Outlet Check Valve Assembly for model (Quaternary & Isocratic) CHR02-000 G1311-60012 Change every 6 months 1200/1100/1050 AS-250-1417 Change every 6 months Oulet Check Valve cartridge only for model 1200/1100/1050 n/a Outlet Check Valve Assembly (Binary Pump) for 1200/1100/1050 AS-250-1397 G1312-60012 Change every 6 months (400 bar) AS-250-1496 Change every 6 months Sieves, Outlet Valve 10/pk for model 1200/1100 5063-6505 Active Inlet Cartridge (400 bar) for model 1200/1100/1050 CHR07-001-10 5062-8562 Change every 6 months AS-250-2300 Change every 6 months Check cleanliness of flow cell every 6 months D2 Lamp comparison test data Instrument: Agilent MWD G1365D D2 lamp: Agilent Chrom4 CL4-AgDADLL Self test mode: Single Test items Requirement Agilent™ Result Chrom4 Result Data measured passed Data measured passed Min. intensity (190nm - 220nm) > 2000 cts passed passed Min. intensity (221nm - 350nm) > 5000 cts 9062 cts passed 10944 cts passed Min. intensity (351nm - 500nm) > 2000 cts 16416 cts passed 18960 cts passed Min. intensity (501nm - 950nm) > 4000 cts 10508 cts passed 11426 cts passed Max. intensity (190nm - 350nm) < 450000 cts 10035 cts passed 9709 cts passed Max. intensity (700nm - 950nm) < 300000 cts 50824 cts passed 65979 cts passed < 1200000 cts 40411 cts passed 39539 cts passed Max. intensity (D2 alpha line) 485.5 ~486.5 nm 98459 cts passed 107132 cts passed Wavelength at 486.0nm 655.6 ~656.6 nm 485.94 nm passed 485.76 nm passed Wavelength at 656.1nm -1 ~ 1 nm 656.07 nm passed 655.94 nm passed Holmium test < 0.002 AU 0.30 nm passed 0.40 nm passed Spectral flatness ≤ 0.02 mAU 0.0000 AU 0.0000AU 0.0124 mAU 0.0115 mAU ASTM noise (20 min. at 254nm) Ordering Information - Lamps Deuterium Lamps Replacement OEM Part Typical Maintenance VWD UV Lamp / 2000hr, Agilent™ models 1200, 1100, G1314 Part Number G1314-60100 Schedule Diode Array Lamp / 2000hr, Agilent™ 1200, 1100, G1315A, G1365A CL4-1314 5182-1530 Change every 2000 hrs or 6 CL4-AgDADLL months, whatever comes first For replacement Electron Multipliers, please visit chrom4.com 68 [email protected] :: www.chrom4.com

LC Accessories REPLACEMENT PARTS - SHIMADZU D2 Lamp comparison test data Instrument: Shimadzu LC SPD-10Avp D2 lamp: Shimadzu 228-34016-02 Chrom4 CL4-SPD10-20 Test items Requirement Shimadzu Result Chrom4 Result Intensity at 220.0nm > 800 cts Data measured passed Data measured passed 1 h. drift ave. (1 h at 250.0nm) < 3 x 10-4 Au/HR (Ref.) passed (Ref.) passed 10 min. noise ave. (10 min. at 250.0nm) passed passed < 1 x 10-5 Au 1459 cts 1628 cts 1.1 x 10-4 Au 1.1 x 10-4 Au 0.57 x 10-5 Au 0.57 x 10-5 Au Ordering Information - Lamps Replacement OEM Part Typical Maintenance Part Number 228-34016-02 Schedule Deuterium Lamps CL4-SPD10-20 Change every 2000 hrs or 6 Deuterium Lamp, SPD-10, SPD-10AS, SPD-10AV, 228-34016-00 months, whatever comes first SPD-10Avp, SPD-20 LS-00136PL Deuterium Lamp, SPD-20A Ordering Information - Pump Replacement Parts Pump Replacement Parts Replacement OEM Part Qty Piston Seal-UHMW-PE for models LC-600, LC-9A, LC-10AD, LC-10ADVP, Part Number 228-35146-00 each LC2­ 010, LC-20AB, LC-20AD CHR01-060 Piston Seal-UHMW-PE for models LC-600, LC-9A, LC-10AD, LC-10ADVP, 228-35146-00 \\10 LC2­ 010, LC-20AB, LC-20AD CHR01-060-10 Piston Seal-Teflon for models LC-600, LC-9A, LC-10AD, LC-10ADVP, 228-35146-00 each LC-2010, LC-20AB, LC-20AD CHR01-260 Piston Seal-Teflon for models LC-600, LC-9A, LC-10AD, LC-10ADVP, 228-35146-00 \\10 LC-2010, LC-20AB, LC-20AD CHR01-260-10 Piston for models LC-600, LC-9A, LC-10AD, LC-20AB 228-18523-91 each Piston for models LC-10ADVP, LC-20AD CHR02-060 228-35601-91 each Inlet Check valve assembly for models LC-600, LC-9A, LC-10AD, LC-20AB CHR02-061 228-18522-91 each Inlet Cartridge for models LC-600, LC-9A, LC-10AD, LC-20AB AS-250-1480 n/a each Outlet Check Valve assembly for models LC-600, LC-9A, LC-10AD, LC-20AB AS-250-1460 228-18522-92 each Outlet Cartridge for models LC-600, LC-9A, LC-10AD, LC-20AB AS-250-1485 n/a each Inlet Cartridge assembly for models LC-10ADVP, LC-20AD AS-250-1465 228-390939-92/228-45704-91 each Inlet Cartridge for models LC-10ADVP, LC-20AD AS-250-1481 n/a each Outlet Cartridge assembly for models LC-10ADVP, LC-20AD AS-250-1461 228-34976-91/228-45705-91 each Outlet Cartridge for models LC-10ADVP. LC-20AD AS-250-1486 n/a each AS-250-1466 For replacement Electron Multipliers, please visit chrom4.com [email protected] :: www.chrom4.com 69

LC Accessories REPLACEMENT PARTS - WATERS Ordering Information - PM Kits PM Kits Replacement OEM Part Qty Pump PM Starter Kit for model 600, includes 2 Piston Seals, 2 Inlet CV, Part Number WAT052675 each 2 Oulet CV, 2 Pistons, 4 SST Solvent Filters AS-270-0755 Pump PM Kit Alliance 2690/95, includes: 2 Piston Seals, 4 Pump Head Face WAT270944 each Seals, 2 Inlet/Oulet Cartridges, 2 Pistons, 4SST Solvent Filters AS-270-0944 D2 Lamp comparison test data Instrument: Waters LC 996 D2 lamp: Waters WAT052586 Chrom4 CL4-996-2996 Test items Waters Result Chrom4 Result Data measured passed Data measured passed Intensity at 230nm passed passed Intensity at 240nm 43900 cts passed 52500 cts passed Intensity at 250nm 42800 cts passed 47700 cts passed Intensity at 260nm 35000 cts passed 37800 cts passed Intensity at 270nm 26200 cts passed 29000 cts passed Intensity at 280nm 21100 cts passed 22800 cts passed Intensity at 290nm 18300 cts passed 19400 cts passed Intensity at 254.0nm 18100 cts passed 19500 cts passed 10 min. drift ave. (10 min. at 254.0nm) 34978 cts passed 35023 cts passed 10 min. noise ave. (10 min. at 254.0nm) 0.152 mAU 0.163 mAU 0.0334 mAU 0.0326 mAU Ordering Information - Lamps Replacement OEM Part Typical Maintenance Part Number WAT052586 Schedule Deuterium Lamps CL4-996-2996 WAS081142 Change every 2000 hrs or 6 Deuterium Lamp, Model 996, 2996 CL4-2487-2488 months, whatever comes first Deuterium Lamp, Model 2487, 2488 Replacement parts also available for Dionex, Hitachi, Gilson/Rainin, SSI and Varian Please contact us for part number and ordering information. 70 [email protected] :: www.chrom4.com

LC Accessories REPLACEMENT PARTS - WATERS Ordering Information - Pump Replacement Parts Pump Replacement Parts Replacement OEM Part Qty Part Number WAT033679/ 700000253 each Inlet Check Valve Assembly for models M45, 45G, 510, 515, 590, 1515, 1525 AS-250-1120 WAT025216/ 700000253 each (Analytical), 6000, 6000A, LCM1 n/a \\5 Outlet Check Valve Assembly w/Adapter Seal for models M45, 45G, 510, 515, AS-250-1125 590, 1515, 1525 (Analytical), 6000, 6000A, LCM1 WAT033679/ 700000253 each WAT025216/ 700000253 each Adapter Seal fo Outlet CV for models M45, 45G, 510, 515, 590, 1515, 1525 AS-250-1135-5 n/a \\5 (Analytical), 6000, 6000A, LCM1 WAT270941 each Inlet Check Valve Assembly for models M501, 600, 600E, 610 AS-250-1020 WAT022934/ WAT022946 each Outlet Check Valve Assembly for models M501, 600, 600E, 610 AS-250-1127 WAT022934/ WAT022946 \\10 Adaptor Seal for Outlet CV models M501, 600, 600E, 610 AS-250-1137-5 WAT026613 each Inlet/Outlet Check PEEK cartridges for models 2690/95, 2790/95/96, AS-250-1070 WAT026613 \\10 Direct replacement WAT270938 each Piston Seal - UHMW-PE for models M45, 45G, 501, 510, 515, 590, 600, CHR01-090 WAT270938 \\10 600E, 610, 1515, 1525 (analytical), 6000, 6000A, LCM1 WAT271018 each WAT271018 \\10 Piston Seals - UHMW-PE for models M45, 45G, 501, 510, 515, 590, 600, CHR01-090-10 WAT271017 each 600E, 610, 1515, 1525 (analytical), 6000, 6000A, LCM1 WAT270940 each WAT270939 each Piston Seal - Teflon for models M45, 45G, 501, 510, 515, 590, 600, 600E, CHR01-290 700002599 \\2 610, 1515, 1525 (analytical), 6000, 6000A, LCM1 700002598 \\2 700003616 \\7 Piston Seal - Teflon for models M45, 45G, 501, 510, 515, 590, 600, 600E, CHR01-290-10 WAT026524 each 610, 1515, 1525 (analytical), 6000, 6000A, LCM1 WAT025656 each Piston Seal - UHME-PE for models Alliance 2690/95, 2790/95/96 AS-200-0210 WAT270959 each WAT700002596 each Piston Seals - UHME-PE for models Alliance 2690/95, 2790/95/96 AS-200-0215 WAT700003563 each WAT700005164 each Seal Wash Piston Seal - UHMW-PE for models Alliance 2690/95, 2790/95/96 AS-200-0220 Seal Wash Piston Seals - UHMW-PE for models Alliance 2690/95, 2790/95/96 AS-200-0225 Seal Wash Face Seal - UHMW-PE for models Alliance 2690/95, 2790/95/96 AS-200-0230 Wash Tube Seal- UHMW-PE for models Alliance 2690/95, 2790/95/96 AS-200-0240 Pump Head Face Seal-UHMW-PE for models Alliance 2690/95, 2790/95/96 AS-200-0250 Head Plunger Seal for models Acquity, nanoAcquity CHR01-091 Wash Seal for models Acquity, nanoAcquity CHR01-092 SS Solvent Filter for models Acquity, nanoAcquity CHR07-023 Piston with Sapphire Rod for models M45, 45G, 501 CHR02-091 Piston with Sapphire Rod for models M510, 515, 590, 600, 610, 1515, 1525 CHR02-092 (analytical),6000, 6000A, LCM1 Piston with Sapphire Rod for models Alliance 2690/95, 2790/95/96 CHR02-093 Primary Check Valve Assembly, Acquity UPLC AS-250-1497 Acquity Intelligent Valve Assembly AS-250-1498 Check Valve Double Ball & Seat, Acquity UPLC AS-250-1499 [email protected] :: www.chrom4.com 71

LC Accessories Isocratic Model 501 Gradient System 500G Pumps ASI HPLC Pumps • Self-priming pump head requires no manual priming HPLC Pumps • Patented “Floating Pump Seal” design for Part No. Description extended seal wear AS-501-0002* Model 501, Micro Head, Standard • Available with micro, analytical, semi-prep and AS-501-0002HP* Model 501, Micro Head, High Pressure AS-501-0000* Model 501, Analytical Head, Standard prep pump heads AS-501-0020* Model 501, Semi Prep Head, Standard AS-501-0040* Model 501, Prep Head, Standard • UHPLC versions for pressures up to 15,000 psi AS-500G-0000* Gradient System, Analytical Head • Programmable solvent compressibility compensation AS-500G-0002* Gradient System, Micro Head • Easy maintenance and service for all wetted parts AS-500G-0002HP* Gradient System, Micro Head, High Pressure • Integral prime/purge assembly for quick AS-500G-0020* Gradient System, Semi-Prep Head AS-500G-0040* solvent change Gradient System, Prep Head The ASI HPLC pump is designed with the chromatographer in * Please specify your local required line voltage for the above items mind. It can be used as a reliable low cost isocratic pump or integrated into a gradient HPLC System. In either case, the Replacement Pump Head Assemblies pump incorporates several key features which make it truly one of a kind in the world of HPLC Pumps. Part No. Description AS-A500-1000 Isocratic Model 501 AS-A500-1001 Spring Housing w/ Micro 2mL AS-A500-1002 Pump Head Assembly The Model 501 is a high performance, compact solvent delivery AS-A500-1003 Spring Housing w/ Analytical 10mL module that has been designed to compete with the most Pump Head Assembly expensive pump systems on the market in reliability and Spring Housing w/ Semi-Prep 20mL performance without the high cost. A dual piston design with Pump Head Assembly solvent compressibility feedback assures virtually pulse-free and accurate solvent delivery. The Model 501 has all of the features Spring Housing w/ Prep 80mL of the Model 500, but without gradient programming capability. It Pump Head Assembly may be used as an economical stand alone pump for isocratic operation, or in conjunction with a Model 500 for gradient opera­tion. Replacement Check Valves Model 500 Part No. Description AS-A500-1133 The Model 500 has been designed to convert the Model 501 2mL Pump Head Inlet/Outlet Check Valve Cartridge from an isocratic pump to a gradient HPLC system. Full AS-A500-1050 10mL Pump Head Inlet/Outlet Check Valve Cartridge, operation and gradient control programming is achieved through AS-A500-1060 20mL and 80mL Pump Head Outlet Check Valve Cartridge the keypad on the front panel of the Model 500. The Model 500 20mL and 80mL Pump Head Inlet Check Valve Cartridge includes a high pressure integral static mixer, connecting tubing, and all the accessories necessary to quickly convert the Model Replacement Piston 501 to gradient operation. Part No. Description Model 500G AS-A500-1117 2mL Pump Head Piston Assembly, Ceramic The Model 500G is a complete high pressure gradient HPLC AS-A500-1116 10mL Pump Head Piston Assembly, Sapphire system. The system includes one Model 501, one Model 500, AS-A500-1024 20mL Pump Head Piston Assembly, Sapphire and all necessary electrical and plumbing connections. AS-A500-1044 80mL Pump Head Piston Assembly, Ceramic Replacement Piston Seals Pump Head Flow Rate Range Pressure Part No. Description Rating Micro Head 0.5 µL/min. to 4 mL/min AS-A500-1009-U-2 2mL Pump Head Piston Seal Assembly, UHMW /2pk 6,000 PSI Max AS-A500-1009-U-10 2mL Pump Head Piston Seal Assembly, UHMW /10pk Analytical Head 1.0 µL/min. to 10 mL/min 6,000 PSI Max AS-A500-1006-T-2 10mL Pump Head Piston Seal Assembly, Teflon /2pk 2,500 PSI Max AS-A500-1006-T-10 10mL Pump Head Piston Seal Assembly, Teflon /10pk Semi-Prep Head 0.050 mL/min. to 20 mL/min 1,000 PSI Max AS-A500-1006-U-2 10mL Pump Head Piston Seal Assembly, UHMW /2pk AS-A500-1006-U-10 10mL Pump Head Piston Seal Assembly, UHMW /10pk Prep Head 0.100 mL/min. to 80 mL/min AS-A500-1026-U-2 20mL Pump Head Piston Seal Assembly, UHMW /2pk AS-A500-1026-U-10 20mL Pump Head Piston Seal Assembly, UHMW /10pk 72 [email protected] :: www.chrom4.com

LC Accessories Precolumn, Inline Filters and Guard Columns Precolumn and Inline Filters • Negligible effect on column performance • Universal fit • Economical protection against micro-particles • Extends LC/UHPLC column lifetime UltraShield Precolumn Filter PrimeLine In-Line Filter • PrimeLine in-line filters were engineered and designed • UltraShield direct connect precolumn filters were to be used anywhere within an LC system where engineered specifically for use in fast, high efficiency additional filtration is required.requiring high mobile phase separations velocities and ultra high pressure. Direct Connect Precolumn Filter ColumnShield Precolumn Filter • UltraShield direct connect precolumn filters were • ColumnSaver direct connect precolumn filters were engineered specifically for use in high throughput and engineered to minimize dead volume specifically for use routine LC separations. in fast, high efficiency separations requiring high mobile phase velocities. AS-850-1025 AS-850-1010 AS-850-1020-062 AS-850-1050T Ordering Information AS-850-1020-01 Replacement Filter Part No. Description Qty. AS-850-1010 UHPLC UltraShield PreColumn Filter, 0.5 micron x 0.62” dia. SST Frit each AS-850-1020-062 Direct Connect Column Filter, 0.5 micron x 0.62” dia. SST Frit each AS-850-1025-062 In-Line Column Filter, 0.5 micron x 0.62” dia. SST Frit each AS-850-1025 In-Line Column Filter, 0.5 micron x 0.125” dia. SST Frit each AS-850-1025-188 In-Line Column Filter, 0.5 micron x 0.188” dia. SST Frit each AS-850-1050T ColumnShield PreColumn Filter (PEEK), 0.5 micron x 0.62” dia. Titanium Frit each Quick&Easy Guard Column Housing and Cartridge • Extends column lifetime by removing contaminants • Re-usable holder and particulates • Cartridges packed with • Leak free connection to most manufacturers media for maximum column protection UHPLC/HPLC columns, 2.1mm - 4.6mm ID • Simple, quick and easy removal and replacement • Pressure rated to 20,000psi (1,378bar) of disposable cartridge Ordering Information Part No. Description Qty. AS-850-1390-3HH Guard Column Housing, 5mm long each AS-850-1392-04 Guard Column Cartridge, 2mm x 5mm long, packed /5 [email protected] :: www.chrom4.com 73

LC Accessories Flow Splitters and Mixers Ordering Information - Flow Splitters Part No. Description Adjustable Flow Splitters AS-600-PO10-04 Adjustable Flow Splitter, Analytical Range, 0.25mL/min. to 5mL/min. Input Flow, Default Calibration Flow = 1.0mL/min., Split Ratio=5:1 to 100:1 AS-600-PO10-06 Adjustable Flow Splitter, Analytical Range, 0.25mL/min. to 5mL/min. Input Flow, Default Calibration Flow = 1.0mL/min., Split Ratio=1:1 to 20:110:1 Fixed Flow Splitters AS-620-PO10-09 Fixed Flow Splitter, Analytical Range, 0.1mL/min. to 5mL/min. Input Flow, Default Calibration Flow = 1.0mL/min., Split Ratio=20:1 AS-620-PO10-10 Fixed Flow Splitter, Analytical Range, 0.1mL/min. to 5mL/min. Input Flow, Default Calibration Flow = 1.0mL/min., Split Ratio=10:1 AS-620-PO10-11 Fixed Flow Splitter, Analytical Range, 0.1mL/min. to 5mL/min. Input Flow, Default Calibration Flow = 1.0mL/min., Split Ratio=5:1 AS-620-PO10-12 Fixed Flow Splitter, Analytical Range, 0.1mL/min. to 5mL/min. Input Flow, Default Calibration Flow = 1.0mL/min., Split Ratio=3:1 AS-620-PO10-13 Fixed Flow Splitter, Analytical Range, 0.1mL/min. to 5mL/min. Input Flow, Default Calibration Flow = 1.0mL/min., Split Ratio=1:1 AS-620-PO10-CS Fixed Flow Splitter, Analytical Range, 0.1mL/min. to 5mL/min. Input Flow, Default Calibration Flow = 1.0mL/min., Split Ratio=Custom * Does not come with mounting bracket - AS-620-1000 Binary Fixed and Multiport Flow Splitters Fixed/adjustable, semi-prep/prep, multiport, Adjustable Flow Splitter post- and pre-column flow splitters available. Replacement resistor sets also available. Please contact us. HyperShear™ LC and UHPLC Mixers Ordering Information Part No. Description Micro Mixer Assemblies (0.5 μL - 25 μL) , Maximum Pressure 6,000PSI AS-402-0010 Binary Tee Mixer Assembly SS 10 μL AS-402-0025 Binary Tee Mixer Assembly SS 25 μL Low Flow Mixer Assemblies (50 μL - 250 μL) AS-411-0050 In-Line Mixer Assembly SS 50 μL AS-411-0150 In-Line Mixer Assembly SS 150 μL AS-411-0250 In-Line Mixer Assembly SS 250 μL AS-412-0050 Binary Tee Mixer Assembly SS 50 μL AS-412-0150 Binary Tee Mixer Assembly SS 150 μL AS-412-0250 Binary Tee Mixer Assembly SS 250 μL Biocompatible PEEK also available (Low flow, analytical flow & high flow only). Analytical Mixer Assemblies (350 μL and 500 μL) Complete assemblies available. Please enquire. AS-421-0350 In-Line Mixer Assembly SS 350 μl AS-421-0500 In-Line Mixer Assembly SS 500 μL AS-422-0350 Binary Tee Mixer Assembly SS 350 μL AS-422-0500 Binary Tee Mixer Assembly SS 500 μL Comparison data available HyperShear™ Static Mixers vs Agilent 1100 packed bed static mixer and Shimadzu LC-10ADvp. Please contact us. 74 [email protected] :: www.chrom4.com

Dissolution Essentials Quartz Spectrophotometer Cells Chrom4 Quartz Cuvettes (cells) and Glass Cells Chrom4 manufacturing, has many years experience There are three basic designs of aperture. Path lengths below in the production of accessories for UV-Vis-NIR spectroscopy 1mm are measured interferometrically. The rectangular cells to meet all standard procedures found in analytical, are serial numbered and fully traceable. clinical and research laboratories, especially QA environments. A wide range of cells specifically designed for Why is calibration important? spectrophotometers are available from path lengths of 1mm through to 100mm. Place any equipment in the environment of choice, and imme- diately the chosen environment will begin to act on that equip- Quartz Cells ment causing change and ultimately degradation in perfor- mance. This so called drift causes your results to become • Traceable unreliable and no longer “fit for their intended purpose”. While • Calibrated drift cannot be eliminated it can be detected and contained • Certified reference material through the process of calibration. In the pharmaceutical industry, a system where drift has occurred to an unaccept- What is calibration? able level is deemed to be “out of control”. Calibration is the process of establishing how the response of By inference, this same environment will also act on your a measuring device varies with respect to the instrument para- Certified Reference Materials, depending on how they are meter being measured. The usual way to perform calibration stored, etc. These materials must be checked/re-certified to is to measure the parameter (e.g. using a reference material) ensure that the values on which you are depending have not and monitor the instrument response also significantly changed. Ordering Information - Quartz Cells and Reference Standards For Agilent, Buck Scientific, Hitachi, Jasco, Shimadzu, Thermo Scientific and PerkinElmer Part No. (each) Description Rectangular Cells C4QC-1 1 mm UV Quartz Cell C4QC-2 2 mm UV Quartz Cell C4QC-5 5 mm UV Quartz Cell C4QC-10 10 mm UV Quartz Cell C4QC-20 20 mm UV Quartz Cell C4QC-40 40 mm UV Quartz Cell C4QC-50 50 mm UV Quartz Cell C4QC-100 100 mm UV Quartz Cell Reference materials, Spectrophotometer Fluorimeter Colorimeter and flow cells soon available from Chrom4! [email protected] :: www.chrom4.com 75

Dissolution Essentials Introduction to Dissolution Dissolution Essentials - Chrom4: The Dissolution Essential Specialists CHROM4 fulfills the various needs of the dissolution industry The product line consists of items specifically designed to fit by carrying a full line of consumables and accessories for the many different brands of dissolution testing equipment. various brands and models of dissolution testing machines. Dissolution Essentials manufactures products dedicated to CHROM4 produces the highest quality products to the entire specific instruments, such as Distek, Sotax, VanKel and pharmaceutical industry. All of their products are both USP Hanson. and machine manufacturer compliant. In this section, we have included the most common dissolution accessories. For more product information, please browse our website and discover why CHROM4 is the superior alternative for dissolution essentials. What can you expect from us? • Baskets • Intrinsic Dissolution • Basket Shafts • Paddles • Cannula Filters • Sampling Cannula • Capsule Sinkers • Spin Shaft Accessories• • Disintegration Testing • Tubing • Dissolution Media • USP Apparatus 3 • Dissolution Vessels • USP Apparatus 5 & USP Apparatus 6 For more information, please contact us! www.CHROM4.com As well as Caleva, Caliper LifeSciences (Zymark), Electrolab, Erweka, Logan, Pharmatest and Toyama CHROM4 is not affiliated with any of the companies listed above, or their products. Products listed under the companies above are compatible with their instrumentation. 76 [email protected] :: www.chrom4.com

Dissolution Essentials Vessels and Covers Vessels High Precision vessels offer ultimate control of dissolution variables and assist with dissolution instrument calibration Some suppliers offer “one size” generic vessels for all dissolu- to ASTM E 2503-07 requirements. tion baths, however, replacing a vessel with one not specifical- ly designed for the instrument could impair vessel centering. Key Features: NEW Also, larger or smaller vessel inside diameters can change hydrodynamics. Both variables could impact dissolution rates. • Fits directly into the dissolution bath Toyama Vessels • Does not require Centering Ring Now Available CHROM4 dissolution vessels comply with USP (711) and with • Comes standard with individual certificates the associated original equipment manufacturers dimensional specifications. This is extremely important because the ves- • Inside bottom extremely close to true hemisphere - Sphericity sels associated with various dissolution baths are not dimen- sionally the same. • Extremely low tolerance of radius of inside bottom • Inside cylindrical body extremely close to true circle - Circularity • Agreement of both centers of cylindrical body and bottom - Concentricity • Inside cylindrical body extrmely close to true cylinder - Cylindricity High Precision Vessel • Vessel center nearly perfect vertical and guaranteed by the concentricity vessels provide ultimate dimensional accuracy • Extremely low tolerance of inside diameters of cylindrical body • Sphericity less then .10mm • Extremely low vessel-to-vessel variation in configuration • Spherical radius 50.03+/- 0.08mm • ASTM, USP, BP, EP, JP and associated pharmacopeia compliant • Cylindricity less then 0.17mm Ordering Information - High Precision Vessel Part No. (each) Description C4-C900ASTM ASTM High Precision Vessel C4-A900ASTM ASTM Amber High Precision Vessel C4-C900ASTM C4-A900ASTM C4-DKC900PS Ordering Information - Dissolution Vessels and Covers Part No. (each) Description OEM Distek - 3010-0069 C4-DKC900PS Serialized 1000mL Premier Glass Vessel (no centering ring for use in waterless bath dissolution systems) - 3010-0023 C4-DKC900S 1000mL Clear Glass Vessel, No Ring, Serialized 3010-0093 C4-DKA900S 1000mL Amber Glass Vessel, No Ring, Serialized 3350-0096 C4-DKA900CRS 1000mL Amber Glass Vessel, Centered, Serialized 3250-0089 C4-DKC900CRS 1000mL Clear Glass Vessel, Centered, Serialized C4-DKPCR Plastic Centering Ring for Distek Vessels 12-5000 C4-DKCHC Clear Hinged Cover 12-5010 Agilent™ Compatible Parts 12-5035 12-5020 C4-VKC900S 1000mL Clear Glass Vessel, Serialized 12-6300 C4-VKA900S 1000mL Amber Glass Vessel, Serialized 12-6320 C4-VKC900CRS 1000mL Clear Glass Vessel, Centered, Serialized 72-600-510 C4-VKA900CRS 1000mL Amber Glass Vessel, Centered, Serialized 72-600-556 C4-VKCCS Clear Cover w/Paddle Slot C4-VKACS Amber Cover w/Paddle Slot 2503 Hanson 2503-2 C4-HAC900S 1000mL Clear Glass Vessel, Serialized C4-HAA900S 1000mL Amber Glass Vessel, Serialized Sotax C4-SXC900S 1000mL Clear Glass Vessel, Serialized C4-SXA900S 1000mL Amber Glass Vessel, Serialized OWtehesruvpepslysedlisssizoelustiaovnavileasbslee.ls & covers for other dissolution testing (Caleva, Erweka, Pharmatest and Zymark). Please enquire. Ordering Information - Vessel Adaptor Ring Bath Manufacturer Model No. Description OEM Part No. (each) Hanson 69606 C4-HAVAR69606 C4-DKPCR Distek SR8+ Multi-Dose Centering Ring 3350-0096 C4-DKPCR 2100 A, B & C Plastic Centering Ring [email protected] :: www.chrom4.com 77

Dissolution Essentials Baskets, Shafts and Paddles Baskets, Shafts and Paddles CHROM4 offers electropolished 316 stainless steel paddles. Clip style and O-ring style shafts & paddles are precisely CHROM4 offers both clip-on style and O-ring style baskets & manufactured to limit rotational wobble to levels far below basket shafts for most dissolution baths. Both meet the asso- USP (711) specifications. ciated original equipment manufacturers dimensional specifi- cations. Both clip style and O-ring style baskets comply NEW with USP (711) specifications. All CHROM4 baskets are Sotax Basket Shafts made from electropolished, sintered 316 stainless steel. The sintering process essentially creates a welded joint at each and paddles overlapping wire junction. Sintered baskets are stronger and Now Available last longer. They retain original dimensions and geometry and resist wobble. Clip-on style shafts utilize a proprietary, spring C4-DKBSK040 C4-SXBSKSH tempered clip, which retains the original gripping tension far longer than conventional shafts. Ordering Information - Baskets, Shafts and Paddles Part No. (each) Description OEM 2821-0072 Distek 2800-0032 2821-0074 C4-DKBSK040 40 Mesh Basket (USP Standard Size Mesh) C4-DKBSK040ES 40 Mesh Basket (USP Standard Size Mesh), Evolution Series - C4-DKBSPOS 16.5” Basket Shaft, 316 SS, O-Ring/Push On Style 2800-0165 C4-DKBSSCS 16.5” Basket Shaft, 316 SS, Spring Clip Style 3200-0038 C4-DKDS 16.5” Detachable Shaft, 316 SS 3100-0017 C4-DKBAES Basket Adapter for Evolution Series Only 2800-0010 C4-DKBCES Basket Clip for Evolution Series Only C4-DKPDE 16.5” Paddle, 316 SS, Electropolished 12-2100 Agilent™ Compatible Parts - C4-VKBSK040 40 Mesh Basket (USP Standard Size Mesh) 12-2073 C4-VKBSKPL040 40 Mesh Precision Length Basket (USP Standard Size Mesh) 12-2070 C4-VKBSSCSV 14.5” Basket Shaft, 316 SS, Spring Clip Style for V-Series 12-2050 C4-VKBSSCS 15” Basket Shaft, 316 SS, Spring Clip Style 12-1035 C4-VKBSCPOS 15” Conical Basket Shaft, 316 SS, O-Ring/Push On Style 12-1030 C4-VKPDEV 14.5” Paddle, 316 SS, Electropolished for V-Series 65-220-000 C4-VKPDSE 15” Paddle, 316 SS, Electropolished 65-237-000 Hanson 65-700-036 C4-HABSK040 40 Mesh Basket (USP Standard Size Mesh) - C4-HABSSCS 16” Basket Shaft, 316 SS, Spring Clip Style 9353 C4-HAPDE 16” Paddle, 316 SS, Electropolished 2830 Sotax - C4-SXBSK010 10 Mesh Basket (USP Standard Size Mesh) C4-SXBSK020 20 Mesh Basket (USP Standard Size Mesh) C4-SXBSK040 40 Mesh Basket (USP Standard Size Mesh) C4-SXBSKSUP Suppository Basket, Plastic Other basket and basket shaft sizes and styles available. Paddles are also available in different sizes and PTFE coated or molded PTFE. Please enquire. Dissolution Basket Storage Prolong the life of baskets and save money To ensure they meet USP requirements, it is essential that for all tests, baskets are not bent or deformed. The chanc- es of accidentally damaging baskets can be significantly reduced with the use of a good quality basket holder, such as C4-SDBH-12. Key Benefits: • Compatible with all popular basket types Ordering Information (Caleva, Distek,Erweka, Hanson, Pharmatest, Sotax, VanKel, Zymark) • O-rings ensure baskets are held firmly to the holder Part No. (ea) Description • Stores up to 12 baskets C4-SDBH-12 Stackable Dissolution Basket Holder • Fully stackable, letting you transport many baskets at a time • Low cost, it pays for itself by preventing damage to just one basket! 78 [email protected] :: www.chrom4.com

Dissolution Essentials Sampling Cannula and Cannula Filters Sampling Cannula Probe tip filters for most sampling methods are available in and Cannula Filters numerous micron ratings (from 1um to 70um) and they are available in packs of 1000 or 100. These filters are made CHROM4 offers traditional manual and automated sam- from inert, high-density polyethylene. All CHROM4 filters pling cannula for most dissolution baths. In addition to have been specifically formulated to provide the specified “traditional” cannula, CHROM4 offers an extensive selec- micron ratings. tion of “innovative”, DE designed cannula. These cannula All formulations have been verified by porisometer testing. include PEEK cannula, improved filter housing cannula and a low cost cannula which offers significant cost savings without sacrificing quality. Ordering Information - ChromFlo Part No. (each) Description SUN-SRi Equivalent C4-PL010 10 Micron Porous Filters, (JAR/1000) 400105 C4-PL035 35 Micron Porous Filters, (JAR/1000) 400102 C4-PL070 70 Micron Porous Filters, (JAR/1000) 400107 Ordering Information - OEM Sampling Cannula and Cannula Filters 2910-0900 Part No. (each) Description 2910-0906 5720-0203 Distek 5720-0275 5720-0275 C4-DKSSP900 900mL Standard Sampling Probe 5720-0020-1000 5720-0503 C4-DKSSP900-SH 900mL Standard Sampling Probe, Solid Housing 12-3200 C4-DKFD010 10 Micron Filter Disks, (JAR/1000) 17-3300 17-4000 C4-DKFIL010 10 Micron Porous Filters, 1/16”, (JAR/1000) - C4-DKFD045 45 Micron Filter Disks, (JAR/1000) 17-4020 17-4120 C4-DKFIL045 45 Micron Porous Filters, 1/16”, (JAR/1000) 17-4140 C4-DKFIL070 70 Micron Porous Filters, 1/16”, (JAR/1000) - Agilent™ Compatible Parts 95-590-002 95-590-005 C4-VKCAN 4.75’’ (900mL) Bent 316 SS Cannula w/SS luer lock 27-101-091 C4-VKCANAS 4.5” (900mL) Auto-Sampling Cannula 27-101-093 C4-VKFIL010 10 Micron Porous Filters, (JAR/1000) C4-VKFIL045 45 Micron Porous Filters, (JAR/1000) - C4-VKFIL070 70 Micron Porous Filters, (JAR/1000) - C4-VKFT010 10 Micron Filter Tips, (JAR/1000) 27-101-092 C4-VKFT045 45 Micron Filter Tips, (JAR/1000) 27-101-094 Hanson - C4-HACANPT 4.75” (900mL) Bent 316 SS Cannula w/SS perm tip - C4-HACAN 4.75” (900mL) Bent 316 SS 1/8” OD Cannula w/SS luer lock - C4-HACANPEEK 4.75” (900mL) Bent PEEK 1/8” OD Cannula w/PEEK luer lock C4-HAFIL010 10 Micron Porous Filters, (JAR/1000) C4-HAFIL010-16 10 Micron Porous Filters, 1/16”, (JAR/1000) C4-HAFIL045 45 Micron Porous Filters, (JAR/1000) C4-HAFIL045-16 45 Micron Porous Filters, 1/16”, (JAR/1000) C4-HAFIL070 70 Micron Porous Filters, (JAR/1000) C4-HAFIL070-16 70 Micron Porous Filters, 1/16”, (JAR/1000) Sotax C4-SXCANLA 13” (900mL) Bent 316 SS Cannula w/luer adapter C4-SXCANSTS 13” (900mL) 316 SS 1/8” OD Cannula w/stopper and sleeve C4-SXFIL010 10 Micron Porous Filters, (JAR/1000) C4-SXFIL035 35 Micron Porous Filters, (JAR/1000) C4-SXFIL070 70 Micron Porous Filters, (JAR/1000) Cannula stoppers and other sampling cannula and cannula filters also available. Please enquire. C4-HACAN Distek Filter Disc Distek Cannula Filter [email protected] :: www.chrom4.com 79

Dissolution Essentials Sinkers, Basket Shafts and Vessel Stands Sinkers CHROM4 offers the most extensive selection of tablet and capsule sinkers available from a single source. C4-VKBSK-JP Their family of sinkers includes most configurations currently in use worldwide, including helical style, basket style, three- prong style and others. C4-VKPCS Ordering Information - Sinkers Part No. (each) Description OEM 12-3050 Agilent™ Compatible Parts 12-3070 C4-VKPCS 3 Prong Capsule Sinker, Plastic 8280 C4-VKBSK-JP Japanese Pharmacopeia Basket Sinker 7346 Sotax 8283 6881 C4-SXSSCS-4 4 Spring Style Capsule Sinker, 316 SS 7349 C4-SXSSCS-5 5 Spring Style Capsule Sinker, 316 SS 8399 C4-SXSSCS-6 6 Spring Style Capsule Sinker, 316 SS C4-SXSSCS-7 7 Spring Style Capsule Sinker, 316 SS C4-SXSSCS-10 10 Spring Style Capsule Sinker, 316 SS C4-SXORS O-Ring Sinker, 316 SS Basket Shaft/Paddle Holders Ordering Information Ideal for both basket shaft and paddle storage Part No. Description Key Benefits: • Prevents shafts/paddles from becoming bent or scratched • Allows quick and easy transport of many shafts/paddles • Handy end plate to stop shafts/paddles sliding out when carrying • Holds all lengths and types of basket shafts and paddles • Non-corrosive, quality plastic construction • Space-saving, attractive design • Available in both 12 and 16 shaft capacities C4-PDLHLD-12 12 Piece Dissolution Paddle Holder Universal Vessel Stands Part No. (ea) Description OEM DE - For the safe transportation and storage of vessels FEP PTFE Tubing for HP 1/32” Due to their shape, dissolution vessels are difficult to hold C4-PSTFEP (0.8mm) ID x 1/16” (1.6mm) OD, securely. This can often result in scratched, chipped or even 500’ (152m) Roll smashed vessels. The steel/acrylic stands are available in 6 or 8 vessel C4-PSTTYG Tygon Tubing, 1/16” (1.6mm) x - capacities and can hold vessels from any dissolution bath 1/8” (3.2mm) (15m) manufacturer. They can also be stacked together to save bench space and Distek enable you to transport many vessels at a time. C4-DKPPTSA-6 Peristaltic Pump Tubing, 5640-0337 Santoprene (Set of 6) 3 Collar Tygon Auto Analyser 5041-2166 C4-DKTAAT-12 Tubing, 0.08” (2.06mm) ID (Set of 12) VanKel (Agilent™ Compatible Parts) Ordering Information Tubing Peristaltic Pump Tubing, 17-6010 C4-VKPSTSA-8 Silicone, Coded Purple & White (Set of 8) Part No. (each) Description C4-VKPSTTY Colour Coded Sample PTFE 17-6035 Tubing, 0.42” C4-UVS-6 Vessel Stand, 6 Position (10.67mm) ID x 0.066” (1.68mm) C4-UVS-8 Vessel Stand, 8 Position OD, 8 Lengths of 50’ (15m) 80 [email protected] :: www.chrom4.com

GC Columns AVAILABLE IN ANY PHASE YOU REQUIRE Common Phases Polarity Quick View CH-1MS CH-20 CH-35MS CH-STAR-WAX CH-1 CH-1HT CH-35 CH-FFAP CH-88 CH-5DF CH-17 CH-225 polar non polar CH-BAC2 CH-PONA CH-5MS CH-1701 CH-624 CH-BIOHT CH-5 CH-PAH CH-BAC1 GC Columns Dimensions Available FAST I.D. 0.10mm 0.15mm 0.18mm Length from 5 to 60m Film Thickness* from 2 to 15m from 2.5 to 30m from 0.05 to 1.50µm I.D. from 0.05 to 1.00µm from 0.05 to 1.40µm Length Film Thickness* CONVENTIONAL I.D. 0.25mm 0.32mm Length Film Thickness* from 5 to 100m from 5 to 100m from 0.05 to 3.00µm from 0.05 to 5.00µm WIDE BORE 0.45mm 0.53mm from 10 to 75m from 10 to 75m up to 5.00µm up to 5.00µm * Maximum film thickness depends also on the stationary phase type. Chiral columns available in various phases. Please visit www.chrom4.com for information or contact your local distributor. [email protected] :: www.chrom4.com 81

GC Columns CH-1 CH-1 (continued) ChromGC (mm) (m) (µm) Part No. Restek J&W No. iso/prog. (°C) 125-10HB -60to260 / 300 10 2.65 CH1-1053265 - 125-10H5 -60to280 / 300 5.00 CH1-1053500 - 125-1011 -60to340/ 360 125-1017 -60to300 / 320 0.15 CH1-155315 - 125-101J -60to300 / 320 125-1012 -60to300 / 320 CH-1 • Equivalent to USP G1, G2, G9, G38 0.50 CH1-155350 18037 125-1014 -60to260 / 300 125-1015 -60to280 / 300 100% methyl polysiloxane 1.00 CH1-1553100 18052 125-1025 -60to280 / 300 15 1.50 CH1-1553150 18067 125-1037 -60to300 / 320 125-103J -60to300 / 320 (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 3.00 CH1-1553300 10182 125-1032 -60to300 / 320 127-1012 -60to325 / 350 125-103B -60to260 / 300 10 0.10 CH1-101010 41101 127-1013 -60to325 / 350 5.00 CH1-1553500 10177 125-1034 -60to260 / 300 0.10 0.40 CH1-101040 41103 127-1022 -60to325 / 350 125-1035 -60to280 / 300 127-1023 -60to325 / 350 0.53 25 5.00 CH1-2553500 - 125-106J -60to300 / 320 20 0.10 CH1-201010 41102 121-1012 -60to325 / 350 125-1062 -60to300 / 320 0.40 CH1-201040 41104 121-1013 -60to325 / 350 0.50 CH1-305350 18040 125-1064 -60to260 / 300 121-1022 -60to325 / 350 125-1065 -60to280 / 300 121-1023 -60to325 / 350 1.00 CH1-3053100 18055 121-1043 -60to325 / 350 10 0.18 CH1-101818 40101 122-1011 -60to325 / 350 30 1.50 CH1-3053150 18070 - 0.40 CH1-101840 40110 122-1012 -60to325 / 350 2.65 CH1-3053265 122-1013 -60to325 / 350 0.18 20 0.18 CH1-201818 40102 122-1022 -60to325 / 350 3.00 CH1-3053300 10185 0.40 CH1-201840 40111 122-1031 -60to325 / 350 122-1032 -60to325 / 350 5.00 CH1-3053500 10179 122-103E -60to325 / 350 40 0.40 CH1-401840 40112 122-1033 -60to325 / 350 1.00 CH1-6053100 18058 123-1011 -60to325 / 350 0.10 CH1-152510 10105 123-1012 -60to325 / 350 60 1.50 CH1-6053150 18073 123-1013 -60to325 / 350 3.00 CH1-6053300 10188 15 0.25 CH1-152525 10120 123-1014 -60to280 / 300 123-1015 -60to280 / 300 5.00 CH1-6053500 10183 1.00 CH1-1525100 10150 123-1022 -60to325 / 350 123-1026 -60to325 / 350 0.25 25 0.25 CH1-252525 - 123-102F -60to280 / 300 123-1031 -60to325 / 350 0.10 CH1-302510 10108 123-1032 -60to325 / 350 CH-1MS 123-103E -60to325 / 350 ChromGC 30 0.25 CH1-302525 10123 123-1033 -60to325 / 350 0.50 CH1-302550 10138 123-1034 -60to280 / 300 123-1035 -60to280 / 300 1.00 CH1-3025100 10153 123-1052 -60to325 / 350 123-1056 -60to325 / 350 0.10 CH1-153210 10106 123-105F -60to280 / 300 123-1061 -60to325 / 350 0.25 CH1-153225 10121 123-1062 -60to325 / 350 CH-1MS • Equivalent to USP G2 123-106E -60to325 / 350 15 1.00 CH1-1532100 10151 123-1063 -60to325 / 350 100% Dimethylpolysiloxane 3.00 CH1-1532300 10181 123-1064 -60to280 / 300 123-1065 -60to280 / 300 5.00 CH1-1532500 10176 124-1032 -60to300/ 320 (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 124-1034 -60to260 / 300 127-0113 -60to340 / 360 0.25 CH1-253225 - 10 0.40 CH1MS-101040 - 127-0123 -60to340 / 360 0.10 20 0.40 CH1MS-201040 - -60to340 / 360 25 0.52 CH1-253252 - - -60to340 / 360 - -60to340 / 360 1.05 CH1-2532105 - 10 0.18 CH1MS-101818 - 128-0122 -60to340 / 360 0.18 - 128-0122 -60to340 / 360 0.10 CH1-303210 10109 122-0112 -60to340 / 360 20 0.18 CH1MS-201818 122-0131 -60to340 / 360 122-0132 -60to340 / 360 0.25 CH1-303225 10124 12 0.33 CH1MS-122033 - 122-0162 -60to340 / 360 0.20 - 123-0112 -60to340 / 360 0.32 30 0.50 CH1-303250 10139 123-0131 -60to340 / 360 1.00 CH1-3032100 10154 25 0.33 CH1MS-252033 123-0132 -60to340 / 360 123-0162 15 0.25 CH1MS-152525 11620 3.00 CH1-3032300 10184 0.25 30 0.10 CH1MS-302510 11608 0.25 CH1MS-302525 11623 5.00 CH1-3032500 10178 0.25 CH1-503225 60 0.25 CH1MS-602525 - 50 0.52 CH1-503252 18010 15 0.25 CH1MS-153225 11621 1.05 CH1-5032105 0.32 30 0.10 CH1MS-303210 11609 0.25 CH1MS-303225 11624 0.10 CH1-603210 10112 0.25 CH1-603225 10127 60 0.25 CH1MS-603225 - 0.50 CH1-603250 10142 CH-1HT 60 1.00 CH1-6032100 10157 ChromGC 3.00 CH1-6032300 10187 5.00 CH1-6032500 10180 0.45 30 1.27 CH1-3045127 - 2.55 CH1-3045255 - CH-1HT 100% methyl polysiloxane for high temperature (mm) (m) (µm) Part No. Restek J&W No. iso/prog. (°C) 122-1111 -60to380 0.25 15 0.10 CH1HT-152510 - 122-1131 -60to380 30 0.10 CH1HT-02510 - 123-1111 -60to380 123-1131 -60to380 0.32 15 0.10 CH1HT-153210 - 30 0.10 CH1HT-303210 - 82 [email protected] :: www.chrom4.com

CH-5 GC Columns ChromGC CH-5MS CH-5 • Equivalent to USP G27, G36, G41 ChromGC (5%-Phenyl) - methylpolysiloxane (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) CH-5MS • Equivalent to USP G27 127-5012 -60to325 / 350 0.10 CH5-101010 41201 127-501E -60to325 / 350 (5%-Phenyl) - methylpolysiloxane 127-5013 -60to325 / 350 10 0.17 CH5-101017 127-5022 -60to325 / 350 (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 127-5023 -60to325 / 350 121-5522 -60to325 / 350 0.10 0.40 CH5-101040 41203 121-5012 -60to325 / 350 20 0.18 CH5MS-201818 - 121-5542 -60to325 / 350 121-5013 -60to325 / 350 0.18 - 128-5512 -60to325 / 350 0.10 CH5-201010 41202 121-5022 -60to325 / 350 128-5522 -60to325 / 350 20 121-5023 -60to325 / 350 40 0.18 CH5MS-401818 128-5552 -60to325 / 350 128-5012 -60to325 / 350 122-5511 -60to325 / 350 0.40 CH5-201040 41204 128-50H7 -60to325 / 350 12 0.33 CH5MS-122033 - 122-5512 -60to325 / 350 128-5022 -60to325 / 350 122-5516 -60to325 / 350 10 0.18 CH5-101818 - 122-5011 -60to325 / 350 0.20 25 0.33 CH5MS-252033 - 122-5513 -60to325 / 350 122-5012 -60to325 / 350 122-5522 -60to325 / 350 0.18 0.40 CH5-101840 40210 122-501E -60to325 / 350 50 0.33 CH5MS-502033 - 122-5531 -60to325 / 350 0.18 CH5-201818 - 122-5013 -60to325 / 350 122-5532 -60to325 / 350 20 122-5022 -60to325 / 350 0.10 CH5MS-152510 12705 122-5536 -60to325 / 350 0.40 CH5-201840 40211 122-5031 -60to325 / 350 122-5533 -60to325 / 350 122-5032 -60to325 / 350 0.25 CH5MS-152525 12720 122-5552 -60to325 / 350 12 0.33 CH5-122033 - 122-503E -60to325 / 350 15 0.50 CH5MS-152550 12735 122-5561 -60to325 / 350 122-5033 -60to325 / 350 122-5562 -60to325 / 350 0.20 15 0.20 CH5-152020 - 122-5052 -60to325 / 350 1.00 CH5MS-1525100 12750 122-5563 -60to325 / 350 122-5061 -60to325 / 350 123-5511 -60to325 / 350 25 0.33 CH5-252033 - 122-5062 -60to325 / 350 25 0.25 CH5MS-252525 - 123-5512 -60to325 / 350 122-506E -60to325 / 350 123-5513 -60to325 / 350 0.10 CH5-152510 10205 122-5063 -60to325 / 350 0.10 CH5MS-302510 12708 123-5526 -60to325 / 350 123-5011 -60to325 / 350 123-5531 -60to325 / 350 15 0.25 CH5-152525 10220 123-5012 -60to325 / 350 0.25 30 0.25 CH5MS-302525 12723 123-5532 -60to325 / 350 0.50 CH5-152550 12335 123-5013 -60to325 / 350 0.50 CH5MS-302550 12738 123-5536 -60to325 / 350 123-5026 -60to325 / 350 123-5533 -60to325 / 350 1.00 CH5-1525100 10250 123-5031 -60to325 / 350 1.00 CH5MS-3025100 12753 123-5561 -60to325 / 350 123-5032 -60to325 / 350 123-5562 -60to325 / 350 25 0.25 CH5-252525 - 123-503E -60to325 / 350 50 0.25 CH5MS-502525 - 123-5563 -60to325 / 350 123-5033 -60to325 / 350 125-5512 -60to300 / 320 0.10 CH5-302510 10208 123-5053 -60to325 / 350 0.10 CH5MS-602510 - 125-553J -60to300 / 320 123-5062 -60to325 / 350 125-5532 -60to300 / 320 0.25 30 0.25 CH5-302525 10223 123-5063 -60to325 / 350 60 0.25 CH5MS-602525 - 0.50 CH5-302550 12338 124-5012 -60to300 / 320 124-5032 -60to300 / 320 1.00 CH5MS-6025100 - 125-50HB -60to300 / 320 1.00 CH5-3025100 10253 125-501K -60to300 / 320 0.10 CH5MS-153210 12706 125-5017 -60to300 / 320 50 0.25 CH5-502525 - 125-501J -60to300 / 320 15 0.25 CH5MS-153225 12721 125-5012 -60to300 / 320 0.10 CH5-602510 10211 125-503K -60to300 / 320 1.00 CH5MS-1532100 12751 125-5037 -60to300 / 320 0.25 CH5-602525 10226 125-503D -60to300 / 320 25 0.52 CH5MS-253252 - 125-503J -60to300 / 320 60 0.50 CH5-602550 10241 125-5032 -60to300 / 320 0.10 CH5MS-303210 - 125-5034 -60to280 / 300 1.00 CH5-6025100 10256 125-5035 -60to280 / 300 0.32 30 0.25 CH5MS-303225 12724 125-5062 -60to300 / 320 0.50 CH5MS-303250 - 0.10 CH5-602510 10206 125-5065 -60to260 / 280 15 0.25 CH5-153225 10221 1.00 CH5MS-3032100 12754 1.00 CH5-1532100 - 0.10 CH5MS-603210 - 25 0.52 CH5-253252 - 60 0.25 CH5MS-603225 - 0.10 CH5-303210 10209 1.00 CH5MS-6032100 - 0.32 30 0.25 CH5-303225 10224 15 1.50 CH5MS-1553150 12767 0.50 CH5-303250 10239 0.53 30 1.00 CH5MS-3053100 12755 1.00 CH5-3032100 10254 1.50 CH5MS-3053150 12770 50 1.00 CH5-5032100 - 0.25 CH5-603225 10227 60 1.00 CH5-6032100 10257 15 1.27 CH5-1545127 - CH-5DF 0.45 - ChromGC 30 1.27 CH5-3045127 10 2.65 CH5-1053265 - 0.25 CH5-155325 10222 15 0.50 CH5-155350 10237 CH-5DF (Dioxin Furan) 1.00 CH5-1553100 12352 5% silphenylen 1.50 CH5-1553150 10267 0.25 CH5-305325 10225 0.53 0.50 CH5-305350 10240 (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 122-2461 350 / 360 0.88 CH5-305388 - 0.15 CH5DF-602515 - 122-2462 350 / 360 0.25 60 - 30 1.00 CH5-3053100 12355 0.25 CH5DF-602525 1.50 CH5-3053150 10270 3.00 CH5-3053300 10285 5.00 CH5-3053500 10279 1.50 CH5-6053150 10273 60 5.00 CH5-6053500 10283 [email protected] :: www.chrom4.com 83

GC Columns CH-BAC1 ChromGC CH-PONA ChromGC CH-PONA CH-BAC1 100%- Dimethylpolysiloxane Ethanol/Acetone (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 128-1056 -60to325 / 350 123-9134 20to260 / 280 0.20 50 0.50 CHPONA-502050 - 122-10A6 -60to325 / 350 0.32 30 1.80 CHBAC1-3032180 - 125-9134 20to260 / 280 0.25 100 0.50 CHPONA-002550 10195 0.53 30 3.00 CHBAC1-3053300 - CH-BIO-HT CH-35 ChromGC ChromGC CH-BIO-HT CH-35 • Equivalent to USP G42 5% phenyl (35%-Phenyl) - methylpolysiloxane (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) - 360 / 380 122-1932 40to300 / 320 10 0.10 CHBIOHT-103210 10292 - 360 / 380 0.25 30 0.25 CH35-302525 - 123-1932 40to300 / 320 0.32 123-1933 40to300 / 320 0.25 CH35-303225 - 125-1937 40to280 / 300 15 0.10 CHBIOHT-153210 10293 0.32 30 0.50 CH35-303250 - 125-1932 40to300 / 320 0.50 CH35-305350 - 0.53 30 - 1.00 CH35-3053100 CH-PAH CH-35MS ChromGC ChromGC CH-PAH 8-10% phenyl (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) CH-35MS • Equivalent to USP G42 - 340 / 360 0.18 40 0.07 CHPAH-40187 49316 - 340 / 360 (35%-Phenyl) - methylpolysiloxane - 340 / 360 30 0.10 CHPAH-302510 49318 (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 0.25 60 0.10 CHPAH-602510 49317 122-3812 50to340 / 360 122-3832 50to340 / 360 15 0.25 CH35MS-152525 - 122-3862 50to340 / 360 123-3832 50to340 / 360 0.25 30 0.25 CH35MS-302525 - 125-3837 50to320 / 340 125-3832 50to320 / 340 60 0.25 CH35MS-602525 - CH-CARBOWAX-20M 0.32 30 0.25 CH35MS-303225 - ChromGC 0.50 CH35MS-305350 - 0.53 30 1.00 CH35MS-3053100 - CH-CARBOWAX-20M CH-BAC2 ChromGC Polyethylene Glycol • Equivalent to USP G14, G15, G16) (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 112-2032 60to230 / 250 0.25 30 0.25 CH20-302525 - 113-2032 60to230 / 250 0.32 30 0.25 CH20-303225 - CH-BAC2 Ethanol/Acetone (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 123-9234 20to260 / 280 0.32 30 1.20 CHBAC2-3032120 - 125-9234 20to260 / 280 0.53 30 2.00 CHBAC2-3053200 - 84 [email protected] :: www.chrom4.com

GC Columns CH-624 CH-1701 ChromGC ChromGC CH-624 • Equivalent to USP G43 CH-1701 • Equivalent to USP G46 (6% cyanopropyl-phenyl) - methylpolysiloxane (14%-Cyanopropyl-phenyl) - methylpolysiloxane (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 121-1324 -20to260 127-0722 -20to280 0.18 20 1.00 CH624-2018100 40924 122-1334 -20to260 0.10 20 0.10 CH1701-201010 42202 127-0723 -20to280 122-1364 -20to260 121-0713 -20to280 30 1.40 CH624-3025140 10968 123-1334 -20to260 0.40 CH1701-201040 - 122-0712 -20to280 0.25 123-1364 -20to260 122-0713 -20to280 124-1334 -20to260 0.18 10 0.40 CH1701-101840 - 122-0731 -20to280 60 1.40 CH624-6025140 10969 124-1374 -20to260 122-0732 -20to280 125-1334 -20to260 30 1.80 CH624-3032180 10970 125-1364 -20to260 0.25 CH1701-152525 - 122-0733 -20to280 0.32 60 1.80 CH624-6032180 10972 125-1374 -20to260 15 1.00 CH1701-1525100 12050 122-0761 -20to280 122-0762 -20to280 30 2.55 CH624-3045255 - 0.15 CH1701-302515 - 122-0763 -20to280 0.45 75 2.55 CH624-7545255 - 123-0712 -20to280 0.25 30 0.25 CH1701-302525 12023- 123-0713 -20to280 30 3.00 CH624-3053300 10971 600 123-0731 -20to280 123-0732 -20to280 0.53 60 3.00 CH624-6053300 10973 1.00 CH1701-3025100 12053 123-0733 -20to280 123-0753 -20to280 75 3.00 CH624-7553300 10974 0.15 CH1701-602515 - 123-0762 -20to280 123-0763 -20to280 60 0.25 CH1701-602525 12026 125-0712 -20to280 125-0737 -20to280 1.00 CH1701-6025100 12056 125-0732 -20to280 CH-17 0.25 CH1701-153225 12021 ChromGC 15 1.00 CH1701-1532100 12051 0.15 CH1701-303215 - 0.32 30 0.25 CH1701-303225 12024 1.00 CH1701-3032100 12054 CH-17 • Equivalent to USP G3, G17 50 1.00 CH1701-5032100 - (50%-Phenyl) - methylpolysiloxane 0.25 CH1701-603225 12027 60 1.00 CH1701-3032100 12057 (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 15 1.00 CH1701-1553100 12052 127-1712 40to280 / 300 0.10 CH17-101010 - 127-1713 40to280 / 300 0.53 30 0.50 CH1701-305350 12040 0.10 10 0.20 CH17-201020 - 127-1722 40to280 / 300 1.00 CH1701-3053100 12055 121-1722 40to280 / 300 20 0.10 CH17-201010 - 121-1723 40to280 / 300 122-1711 40to280 / 300 0.18 CH17-201818 - 122-1712 40to280 / 300 CH-225 0.18 20 - 122-1713 40to280 / 300 ChromGC 122-1731 40to280 / 300 0.30 CH17-201830 122-1732 40to280 / 300 122-1733 40to280 / 300 0.15 CH17-152515 - 122-1762 40to280 / 300 123-1711 40to280 / 300 15 0.25 CH17-152525 10520 123-1712 40to280 / 300 123-1713 40to280 / 300 0.50 CH17-152550 10535 123-1731 40to280 / 300 123-1732 40to280 / 300 0.25 0.15 CH17-302515 - 123-1733 40to280 / 300 123-1762 40to280 / 300 30 0.25 CH17-302525 10523 125-1712 40to260 / 280 CH-225 • Equivalent to USP G7, G19 125-1732 40to260 / 280 0.50 CH17-302550 10538 125-1733 40to260 / 280 (50%-Cyanopropyl-phenyl) - methylpolysiloxane 125-1762 40to260 / 280 60 0.25 CH17-602525 - 0.15 CH17-153215 - (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 127-2222 45to220 / 240 15 0.25 CH17-153225 10521 0.10 20 0.10 CH225-201010 - 121-2223 45to220 / 240 122-2212 45to220 / 240 0.50 CH17-153250 10536 0.18 20 0.20 CH225-201820 - 122-2231 45to220 / 240 122-2232 45to220 / 240 0.32 0.15 CH17-303215 - 15 0.25 CH225-152525 - 123-2232 45to220 / 240 125-2212 40to200 / 220 30 0.25 CH17-303225 - 0.25 30 0.15 CH225-302515 - 125-2232 40to200 / 220 0.25 CH225-302525 - 0.50 CH17-303250 10539 60 0.25 CH17-603225 - 0.32 30 0.25 CH225-303232 - 15 1.00 CH17-1553100 10552 15 1.00 CH225-1553100 - 0.53 30 1.00 CH225-3053100 - 0.53 30 1.00 CH17-3053100 10555 60 1.50 CH17-6053150 - 1.00 CH17-6053100 10558 For EPA Methods (Drinking & Waste Water), please visit www.chrom4.com [email protected] :: www.chrom4.com 85

GC Columns CH-STAR-WAX CH-FFAP ChromGC ChromGC CH-STAR-WAX • Equivalent to USP G14, G15, G16 CH-FFAP • Equivalent to USP G25, G35, G39 Polyethylene Glycol Nitroterephthalic Acid Modified Polyethylene Glycol (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 127-7012 20to250 / 270 122-3212 40to250 0.10 CHSWAX-101010 - 127-7013 20to250 / 270 15 0.25 CHFFAP-152525 11020 122-3232 40to250 10 0.20 CHSWAX-101020 - 127-7022 20to250 / 270 122-3233 40to250 0.10 0.10 CHSWAX-201010 - 127-7023 20to250 / 270 0.25 30 0.25 CHFFAP-302525 11023 123-3212 40to250 20 0.20 CHSWAX-201020 - 121-7012 20to250 / 270 0.50 CHFFAP-302550 11038 123-3232 40to250 121-7022 20to250 / 270 123-3233 40to250 121-7023 20to250 / 270 15 0.25 CHFFAP-153225 11021 123-3234 40to250 121-7043 20to250 / 270 123-3253 40to250 10 0.18 CHSWAX-101818 - 122-7012 20to250 / 270 0.25 CHFFAP-303225 11024 123-3262 40to250 122-7013 20to250 / 270 123-3263 40to250 0.18 CHSWAX-201818 - 122-7031 20to250 / 270 30 0.50 CHFFAP-303250 11039 123-3264 40to250 0.18 20 0.30 CHSWAX-201830 - 122-7032 20to250 / 270 124-3232 40to250 122-7033 20to250 / 270 0.32 1.00 CHFFAP-3032100 11054 125-3217 40to250 122-7061 20to250 / 270 125-3212 40to250 40 0.30 CHSWAX-401830 - 122-7062 20to250 / 270 50 0.50 CHFFAP-503250 - 125-3231 40to250 122-7063 20to250 / 270 125-3237 40to250 0.25 CHSWAX-152525 - 123-7012 20to250 / 270 0.25 CHFFAP-603225 11027 125-3232 40to250 15 - 123-7013 20to250 / 270 125-3233 40to250 123-7031 20to250 / 270 60 0.50 CHFFAP-603250 11042 0.50 CHSWAX-152550 123-7032 20to250 / 270 123-7033 20to250 / 270 0.15 CHSWAX-302515 - 123-7062 20to250 / 270 1.00 CHFFAP-6032100 11057 123-7063 20to250 / 270 0.25 30 0.25 CHSWAX-302525 10673 124-7032 20to250 / 270 0.45 30 0.85 CHFFAP-304585 - 125-7017 20to250 / 270 0.50 CHSWAX-302550 - 125-7012 20to250 / 270 0.50 CHFFAP-155350 11037 125-7031 20to250 / 270 15 1.00 CHFFAP-1553100 11052 0.15 CHSWAX-602515 - 125-7037 20to250 / 270 125-7032 20to250 / 270 60 0.25 CHSWAX-602525 - 125-7062 20to250 / 270 0.25 CHFFAP-305325 11025 0.53 0.50 CHFFAP-305350 11040 0.50 CHSWAX-602550 - 30 1.00 CHFFAP-3053100 11055 0.25 CHSWAX-153225 - 15 - 1.50 CHFFAP-3053150 - 0.50 CHSWAX-153250 0.15 CHSWAX-303215 - 0.32 30 0.25 CHSWAX-303225 10674 CH-88 ChromGC 0.50 CHSWAX-303250 - 0.25 CHSWAX-603225 - 60 - 0.50 CHSWAX-603250 30 0.85 CHSWAX-304585 - 0.45 0.50 CHSWAX-154550 - CH-88 • Equivalent to USP G5, G8, G48 15 1.00 CHSWAX-1545100 - Biscyanopropyl polysiloxane 0.25 CHSWAX-305325 - 30 0.50 CHSWAX-305350 - (mm) (m) (µm) Part No. Restek J&W iso/prog. (°C) 0.53 1.00 CHSWAX-3053100 - 60to250 60to250 60 1.00 CHSWAX-6053100 - 0.20 50 0.20 CH88-502020 - 60to250 60to250 50 0.20 CH88-502520 - 0.25 60 0.20 CH88-602520 - 100 0.20 CH88-1002520 13199 GC Septa :: Lowest bleed of any septa :: Soft and resealable :: M olded for dimensional consistency giving more injections per septum, thereby costing less Precision molded GC Septa made from 100% Pure Premium Silcone Rubber. Our GC septum delivers the ultimate in low bleed properties and longevity. Our unique formulation and conditioning process is what separates our GC Septa from the rest. Manufacturer Size Quantity Part Number OVSC 9.5mm 50 OVSC 11mm 50 CH-237705 Chrom4 11mm 50 CH-237715 Chrom4 9.5mm 50 CHR-HD-A1544 Chrom4 10mm 50 CHR-HD-A1486 CHR-HD-A1405 86 [email protected] :: www.chrom4.com

GC Accessories GC Accessories Inlet Liners :: Deactivated for maximum inertness :: Meets or exceeds original manufacturers’ specifications :: Use liners packed with fused silica wool for the Agilent™ 7673 Autosampler Description ID (mm) OD (mm) Length (mm) Glass Type Deactivated Agilent™ P/N Part Number Deactivated Injection Port Liners 4.0 6.45 78.5 Borosilicate Yes 5181-3316 IS-4209 Tapered Liner - 1 End Tapered Liner - 1 End w/wool* 4.0 6.45 78.5 Borosilicate Yes 5062-3587 IS-4210 Tapered Liner - Both Ends 4.0 6.45 78.5 Borosilicate Yes 5181-3315 IS-4211 Split/Splitless Liner w/wool* 4.0 6.3 78.5 Borosilicate Yes 19251-60540 IS-4212 Cup Split Liner+ 4.0 6.3 78.5 Borosilicate No 18740-80190 IS-4202 DIrect Liner 1.5 6.45 78.5 Borosilicate Yes 18740-80200 IS-4215 DIrect Connect Dual Taper, Bottom Hole 4.0 6.3 78.5 Borosilicate Yes G1544-80700 IS-4217 Split only, Tapered, Low Pressure Drop 4.0 6.3 78.5 Borosilicate Yes 5183-4647 IS-4220 GP Split, 1 Taper Liner w/wool 4.0 6.3 78.5 Borosilicate Yes 5183-4711 IS-4221 + The Cup Split Liner (IS-4202) contain small amounts of packing (10% CH-1 on 80/100 Chromosorb-WHP) retained by silanized glasswool. * Containing deactivated quartz wool packing to ensure vaporization of your sample before reaching the column entrance. The liner is made of borosilicate glass. Liners for other brands available. See www.chrom4.com for details. [email protected] :: www.chrom4.com 87

GC Accessories Agilent™ Compatible Accessories Ferrules (10 per pack) Ferrule Tubing Style 100% Agilent™ 85% Polyimide Agilent™ Size Size Graphite Part Number 15% Graphite Part Number - Agilent™ Compatible Ferrules 1/16” 0.4mm Agilent Short OV-GF-04 500-2004 OV-MS-04 5062-3508 1/16” 0.4mm - OV-GF-04-HP 500-2114 OV-MS-04-HP 5181-3323 1/16” 0.5mm Agilent Short OV-GF-05 - OV-MS-05 5062-3506 1/16” 0.5mm - OV-GF-05-HP 5080-8853 OV-MS-05-HP 5062-3514 1/16” 0.8mm Agilent Short OV-GF-08 500-2008 OV-MS-08 5062-3538 1/16” 0.8mm - OV-GF-08-HP 500-2118 OV-MS-08-HP 5062-3512 1/16” 1.00mm Agilent Short OV-GF-10 500-2010 OV-MS-10 - 1/16” 1.0mm - OV-GF-10-HP 5080-8773 OV-MS-10HP - 1/16” 1.2mm - OV-GF-12 500-2012 OV-MS-12 - 1/16” 1/16” - OV-GF-01 0100-1326 OV-MS-01 - 1/8” 1/8” - OV-GF-02 0100-1325 OV-MS-02 - 1/4” 1/4” - OV-GF-03 0100-1324 OV-MS-03 - 1/8” 1/16” - OV-GF-68 0100-1336 OV-MS-68 - 1/4” 1/16” - OV-GF-64 0100-1335 OV-MS-64 - 1/4” 1/8” 2-Hole OV-GF-81 0100-1334 OV-MS-81 - 1/16” 0.4mm 2-Hole OV-GF-14 - OV-MS-14 5062-3580 1/16” 0.5mm No-Hole OV-GF-25 - OV-MS-15 5062-3581 1/16” 0.5mm OV-GF-01-NH - OV-MS-01-NH 5181-3308 Ferrules (10 per pack) Ferrule Tubing 60% Polyimide 100% 100% Size Size 40% Graphite Polyimide Teflon Capillary Ferrules 1/16” 0.4mm OV-GH-04 OV-HF-04 OV-TF-04 1/16” 0.4mm OV-GH-04-HP OV-HF-04-HP - 1/16” 0.5mm OV-GH-05 OV-HF-05 OV-TF-05 1/16” 0.5mm OV-GH-05-HP OV-HF-05-HP - 1/16” 0.8mm OV-GH-08 OV-HF-08 OV-TF-08 1/16” 0.8mm OV-GH-08-HP OV-HF-08-HP - 1/16” 1.0mm OV-GH-10 OV-HF-10 OV-TF-10 1/16” 1.2mm OV-GH-12 OV-HF-12 OV-TF-12 Straight Ferrules 1/16” 1/16” OV-GH-01 OV-HF-01 OV-TF-01 1/8” 1/8” OV-GH-02 OV-HF-02 OV-TF-02 3/16” 3/16” - - OV-TF-316 1/4” 1/4” OV-GH-03 OV-HF-03 OV-TF-03 3/8” 3/8” - - OV-TF-138 1/2” 1/2” - - OV-TF-13 Electron Multipliers Analyzer Type Technique Part Quadrupole GC-MS Number Instrument 5971, 5972, 5973, 5975, LC/MSD DT-2300A 88 [email protected] :: www.chrom4.com

GC Accessories GC Accessories Press-Fit Connectors :: Easy to handle :: A simple pressure to assure a perfect seal :: Easy to install ChromGC Press-Fit connectors allow you to simply connect, with a tight seal, different columns or Retention Gaps together in many ways. Our Press-Fit connectors are custom-made to fit any tubing size and to ensure the minimal dead volume. Press-Fit Union linear connectors, ideal to connect two columns or a Retention Gap to the analytical column. Press-Fit “Y” three way connectors, ideal to connect two analytical columns to a single injector port or split the exit of one column to a dual detector GC system. Many other configurations are possible using ChromGC “Y” Press-Fit. ChromGC also produces personalized Multiways Press-Fit connectors for advanced analytical system configurations as MDGC and other custom settings. Guard Columns - Retention Gaps :: Inert :: Pretested :: Increased analytical column lifetime Guard columns consist of 5 meters of deactivated fused silica supplied with high temperature Length I.D. Catalog string. All guard columns are extremely inert and each is pretested. mm Number 5 Meters Guard columns are useful in protecting your analytical column from harmful non-volatile 5 Meters 0.18 CH-6180-5 contaminates that are commonly found in many environmental and industrial samples. 5 Meters 5 Meters 0.25 CH-6250-5 Attaching the guard column to the front of the analytical column using fused silica connectors, or zero dead volume union, will extend your column’s lifetime. The non-volatile contaminants are 0.32 CH-6320-5 deposited onto the guard column. 0.53 CH-6530-5 Inlet Gold Seals Instrument Description Pack Part Size Number For Agilent™ Compatible Parts 7890/6890/6850 0.8mm Inlet Seal, Gold-plated Splitless, Gold-plated Washers 2 OV-4300 0.8mm Inlet Seal, Gold-plated, Splitless, Gold-plated Washers 10 OV-4300-10 0.8mm Inlet Seal, Gold-plated, Split Double Groove (Cross), Gold-plated Washers 2 OV-4400 0.8mm Inlet Seal, Gold-plated, Split Double Groove (Cross), Gold-plated Washers 10 OV-4400-10 Replacement Gold-plated Washers for Inlet Seal 12 OV-4800 GC Septa Manufacturer Size Quantity Part Number Perkin Elmer 11mm 50 Shimadzu Plug 50 CHR-HD-P1544 CHR-HD-S1544 [email protected] :: www.chrom4.com 89

GC Columns XTRM (Extreme) Columns What is an XTRM (Extreme) column? XTRM columns are wall coated open tubular (WCOT) columns consisting of stainless steel tubing that has a special internal surface treatment. This special surface treatment makes the surface similar to deactivated fused silica. The unique surface treatment process permits us to offer XTRM columns in a wide range of internal diameters and phases. Because the special surface treatment permeates the stainless steel surface, rather than the conventional coating, the layer is particularly easy to bend, so the tubing can be coiled to very small diameters. Compare XTRM columns and fused silica columns: • Metal columns can be coiled under 4.5 inches without breaking, ideal for small instruments. • Metal tubing allows XTRM columns to be used to higher temperatures (430°C) than fused silica columns (standard rating is 380°C). • Coating efficiency (plates/meter) of XTRM columns is comparable to that of fused silica. • D ormancy of XTRM columns and fused silica columns is comparable, due to the unique properties of the special surface treatment in XTRM columns. • XTRM columns will not break under stress. XTRM column phases available from Chrom4: • CH-1-XTRM • CH-35-XTRM • CH-5-XTRM • CH-SE52-XTRM • CH-SE54-XTRM • CH-624-XTRM • CH-17-XTRM • CH-SimDis-XTRM For dimensions and information of the phases listed above, please visit www.chrom4.com or ask your local distributor. 90 [email protected] :: www.chrom4.com

GC Columns Applications Compound Column Compound Column Acetal - Ethyl Acetate CH-STAR-WAX CH-5 - EPA Acetic Acid monitoring CH-FFAP (inquire) Haloethers Method 611-8110 Alcohol Ethoxylates CH-5HT Alkyl Naphthalens CH-5 Halogenated Volatiles CH-624 - EPA Anaesthetics Basic Drugs CH-5 Method 551 Aroclor 1254 - 1260 PCBs Quick Screening CH-5 Ketones Basic Drugs Underivatized CH-5 CH-1 Benzene aromatic volatile compounds in air CH-STAR-WAX Natural Gas Hydrocarbons Benzyl Alcohol impurities CH-FFAP (inquire) CH-1 Bergamot Oil FAST-GC CH-1701 Nitrogen-Phosphorus Pesticides Herbicides Bergamot Oil FAST-GC CH-WAX (inquire) (Triazines) CH-STAR-WAX Biodiesel - EN 14105 - ASTM D6584 CH-BIO EPA Method 619 Biodiesel FAMEs CH-BIO Nitrosamines Biodiesel Glycerin - Triglycerides CH-BIO CH-5 - EPA Method 607 Blood Alcohol (Headspace) CH-BAC1 & CH-BAC2 Nitrosamines Blood Alcohols - HS CH-BAC1 & CH-BAC2 CH-STAR-WAX Blood Alcohols - HS dual column CH-BAC1 & CH-BAC2 Olive Oil FAMEs (Fatty Acid Methyl Esters) BTEX FAST-GC CH-624 Cis-Trans Isomers - CH-88 Butter Triglycerides CH-1 Chamomile Essential Oil FAST-GC CH-STAR-WAX (inquire) Organic Acids CH-FFAP Chamomile Essential Oil FAST-GC Comparison CH-1701 Chlorinated Hydrocarbons CH-5 - EPA Method 612 Organochlorine Pesticides CH-5 - EPA Method Critical Flavor & Fragrances Compounds CH-FFAP 608-8081 Cyclic Hydrocarbons CH-1 Organophosphorus Pesticides Determination of mineral oil hydrocarbons in CH-1HT PAH Polycyclic Aromatic Hydrocarbons CH-1 and CH-1701 vegetable oils Diethylene Glycol and Diols CH-FFAP PAHs CH-PAH - EPA Method Dimethylanilines CH-STAR-WAX 610-8100 Dioxins and Furans GC-MS CH-5MS PAHs Drugs and Adulterants CH-5 (inquire by GC-MS) CH-5MS - EPA 610 - 8100 Drugs of Abuse - Basic Drugs CH-1 PAHs - 8270 Drugs of Abuse - Cannabinoids CH-1 PAHs EPA 8270 - Helium Carrier Gas Drugs Precursors CH-5 PAHs FAST-GC CH-PAH (inquire) FAMEs (Fatty Acid Methyl Esters) C4 - C18 CH-STAR-WAX Pesticides FAST-GC EPA Methods 610 - 8100 FAMEs (Fatty Acid Methyl Esters) C6 - C24 CH-STAR-WAX Phenols - 8270 FAMEs (Fatty Acid Methyl Esters) Standard CH-88 Phenols Mixture Phthalate Esters CH-PAH (inquire) FAMEs (Fatty Acid Methyl Esters) Standard CH-88 (inquire) Purgeable Aromatics Mixture FAST-GC CH-PAH FAMEs and CLA isomers CH-88 Residual Solvents UPS 467 FAMEs cis trans Isomers CH-88 Serum Volatiles Control CH-PAH (inquire) Flavor & Fragrances Compounds CH-FFAP Solvents Food Packaging Residual Solvents CH-1701 Sulfur compounds in coffee aroma CH-1701 (inquire) Fragrance Allergens FAST-GC CH-1701 (inquire) USP 467 Residual Solvents (OVIs) FAST-GC Fragrance Allergens FAST-GC CH-STAR-WAX (inquire) Headspace CH-1 Free Acids CH-FFAP VOCs in Drinking Water Free Fatty Acids in Whisky CH-FFAP CH-5 - EPA Method 604 Vodka Volatile Acidic Compounds CH-1 - EPA Method 606 Volatile Free Acids Whisky direct split injection CH-STAR-WAX Xylene Isomers EPA Method 602 CH-624 CH-BAC1 CH-WAX CH-FFAP CH-624 (inquire) (G43 Phase) CH-624 - EPA Method 524-3 CH-STAR-WAX CH-FFAP CH-FFAP CH-624 CH-FFAP [email protected] :: www.chrom4.com 91

FUTURE DEVELOPMENTS: Sample Transfer DataM8Riks Ritter Robotic Tips Biolink: Durable U(H)PLC Fittings Electron Multipliers DT-2300A (Agilent GC-MS) (U) HP LC-MS Columns: uattro ISOSCELES PELLICULAR CORE Bulk Silica 92 [email protected] :: www.chrom4.com

[email protected] :: www.chrom4.com 93

CHROM4 GmbH, Haupstrasse 365, Suhl-Wichtshausen, Thüringen, Germany, D-98530 HRB, Registergericht Jena, [email protected]