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AMT-Enviroclass-Catalog_FINAL-small

Published by Canadian Life Science, 2020-09-21 15:20:39

Description: AMT-Enviroclass-Catalog_FINAL-small

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QUALITY POLICY AMT is committed to providing world-class innovative products that uniquely fill the growing needs of small molecule and large molecule separation scientists. We take pride in delivering products that exceed customers’ expectations on quality and delivery time and collaborate to break down any barriers that would prevent an exceptional customer experience. We continually strive to improve our organization to stay focused on safety, quality, and cost. We embrace ISO9001 standards in our work systems and daily work. We pledge to have dynamic leadership promoting culture of excellence embedded in every employee. INNOVATION YOU CAN TRUST - PERFORMANCE YOU CAN RELY ON

ENVIRONMENTAL SOLUTIONS HALO® ENVIROCLASS is the newest family of products in Advanced Materials Technology’s product line. Comprised of chromatography columns designed specifically to address environmental analysis, HALO® delivers results. Built upon proven Fused- Core® technology, HALO® ENVIROCLASS delivers high efficiency separations with the rugged performance required to meet challenging environmental sample matrices. From performance designed application specific phases to separate per-and polyfluorinated alkyl substances (PFAS) and polycyclic aromatic hydrocarbons (PAH), HALO® ENVIROCLASS represents more than method assured products. It offers a suite of solutions for other persistent, high environmental-impact contamination agents such as pesticides, mycotoxins, herbicides and more all based upon HALO’s 15 years of innovative and trusted technology all with the understanding of the unique needs of the environmental laboratory.   |  1

HALO® PFAS HALO® PFAS Designed for separation of novel and legacy short CH3 chain and long chain PFAS compounds containing O Si C18H37 branched and linear isomers, along with EPA methodology requirements in mind, HALO® PFAS CH3 offers a holistic solution. With both a PFAS specific delay column optimized to prevent background HALO® PFAS DELAY CH3 PFAS contamination from interfering with the sample results and an analytical column for PFAS sample CH3 separation and detection, the HALO® PFAS solution O Si (CH2)n delivers excellent selectivity, peak shape and necessary retention to perform fast, high resolution CH3 separations in EPA methods 537.1, 533 and 8327. The HALO® PFAS solution is different from other APPLICATIONS C18 offerings in that it is quality assured for PFAS analysis providing confidence it will meet • EPA 533 application demands. • EPA 537.1 • Application-assured through method • EPA 8327 • Emerging PFAS qualified lot analysis • Optimal 2.7 µm Fused-Core® particle for rugged, reliable performance delivering high efficiency, low back pressure separations • Endcapped alkyl phases for high sensitivity (no bleed) LCMS analysis • Pressure limit: 600 bar/9000 psi |2     fused-core.com

RAPID ANALYSIS OF 33 PFAS COMPOUNDS IN UNDER 5 MINUTES High speed separation of 33 PFAS species found in EPA 537.1, EPA 533, and EPA 8327, completed in under 5 minutes. 550000 12,13 PEAK # COMPOUND PEAK # COMPOUND 500000 1 PFBA 18 PFOS 2 4:2FTS 19 450000 3 PFPeA 20 9Cl-PF3ONS 4 PFBS 21 8:2FTS 400000 5 PFHpS 22 PFNS 6 PFPeS 23 PFDA 350000 16 7 PFMPA 24 300000 8 PFHxA 25 N-MeFOSAA 250000 15 9 26 PFNA 200000 10 PFEESA 27 NFDHA 150000 11 18 20-22 11 HFPO-DA 28 PFUnA 100000 25-28 12 50000 30 13 PFHxS N-EtFOSAA 14 NaDONA 6:2FTS 0 19 31 15 ADONA 16 12 3 4 6 10 14 17 23 29 3233 17 FOSA 1.0 5 78 9 24 5.0 PFOA 4.0 PFMBA 2.0 3.0 PFHpA 29 11Cl-PF3OUdS Time (min) 30 PFTrDA 31 PFDoA TEST CONDITIONS Flow Rate: 0.4 mL/min 32 PFTeDA Analytical Column: HALO® PFAS, 2.7 µm, 2.1 x 100 mm Pressure: 479 bar 33 PFDS Delay Column: HALO® PFAS Delay, 2.7 µm, 3.0 x 50 mm Temperature: 35 °C Mobile Phase A: 10 mM Ammonium Acetete Injection Volume: 2.0 µL Mobile Phase B: Methanol Sample Solvent: Methanol (96%) Water (4%) Gradient: 33-98 %B in 4.0 min: 98-100 %B in 0.1 min.; hold @ 100 %B for 2min. PFAS ANALYSIS ACCORDING TO EPA 8327 HALO® PFAS provides a high resolution separation for EPA 8327 used for the analysis of non-potable water samples. 40000 10 PEAK # COMPOUND PEAK # COMPOUND 12 1 PFBA 13 PFNA 16 2 4:2FTS 14 PFOS 3 PFPeA 15 PFNS 30000 4 PFBS 16 PFDA 5 PFHpS 17 8:2FTS 8 14 18 6 PFPeS 18 11 7 PFHxA 19 N-MeFOSAA 8 PFHpA 20 6:2FTS 20000 13 22 24 9 PFHxS 21 PFUnA 10000 17 21 10 FOSA 22 1 5 15 20 23 11 PFOA 23 N-EtFOSAA 0 6 9 12 PFDS 24 PFDoA PFTrDA 4 19 PFTeDA 3 7 2 2.5 5.0 7.5 10.0 12.5 min Time (min) TEST CONDITIONS Flow Rate: 0.4 mL/min Analytical Column: HALO® PFAS, 2.7 µm, 2.1 x 100 mm Initial Back Pressure: 485 bar Delay Column: HALO® PFAS Delay, 2.7 µm, 3.0 x 50 mm Temperature: 35 °C Mobile Phase A: 10 mM Ammonium Acetete Injection Volume: 2.0 µL Mobile Phase B: Methanol Sample Solvent: Methanol (96%) / Water (4%) Gradient: 33-98 %B in 18 min. For complete method information see Technical Library at fused-core.com   |  3

PFAS ANALYSIS ACCORDING TO EPA 533 EPA Method 533 is for drinking water analysis and targets both short and long chain PFAS compounds. 16 PEAK # COMPOUND PEAK # COMPOUND 19 14 PFOA 40000 23 24 1 PFBA 15 PFmbA 16 PFNA 2 4-2FTS 17 PFOS 18 9 14 21 3 PFPeA 19 9Cl-PF3ONS 20 PFDA 30000 4 PFBS 21 8-2FTS 20000 22 6-2FTS 10000 15 5 PFHpS 23 NFDHA 24 PFUnA 0 12 25 6 PFPeS 25 13 11Cl-PF3OUdS 15 17 22 7 PFmpA PFDoA 6 10 11 4 3 78 8 PFHxA 2 5.0 18 20 9 PFEESA 2.5 7.5 10.0 12.5 10 HFPO-DA PFHpA Time (min) min 11 TEST CONDITIONS 12 PFHxS Analytical Column: HALO® PFAS, 2.7 µm, 2.1 x 100 mm Delay Column: HALO® PFAS Delay, 2.7 µm, 3.0 x 50 mm Flow Rate: 0.4 mL/min 13 ADONA Mobile Phase A: 10 mM Ammonium Acetete Initial Back Pressure: 485 bar Mobile Phase B: Methanol Temperature: 35 °C Gradient: 33-98 %B in 18 min. Injection Volume: 2.0 µL Sample Solvent: Methanol (96%) / Water (4%) ANALYSIS OF PFAS IN WELL WATER Method 533 complements EPA Method 537.1 and can be used to test for 11 additional PFAS species.Here we show a clear separation of the branched and linear isomers of PFAS species PFHxS, found in a spiked well water sample. x10 4 -ESI TIC MRM Frag=166.0V (** -> **) Well_05.d x10 4 -ESI TIC MRM Frag=166.0V (** -> **) Well_05.d 16 PEAK # 1COMPOUND PEAK # COMPOUND 3.4 1 16 PFOA 3.4 16 17 PFHpS 3.2 3.2 18 PFNA 3 1 PFBA 19 PFOS 3 20 2.8 2.8 13,14 13,14 21 9Cl-PF3ONS 2.6 12 12 22 8-2FTS 2.4 2.6 20 9 20 2 PFMPA 23 PFDA 2.2 1 21 24,25 27 PFPeA 24 92.4 2 22 PFBS 25 NMeFOSAA 2 PFMBA 26 NEtFOSAA 1.8 2.2 PFEESA 27 1.6 28 PFUnA 1.4 2 21 24,25 237 29 11Cl-PF3OUdS 1.2 22 1.8 PFDoA 1 PFTrA 0.8 1.6 PFTA 10.6 1.4 26 28 29 4 20.4 1.2 26 28 29 0.2 0 3 61 318 19 4,5 6 18 19 5 1234 4,5 0.8 23 7 8 15 17 10 0.6 11 15 17 10 11 70.84 23 6 0.2 5 6 7 80 9 10 11 12 13 14 15 16 17 18 19 x10C4oun-EtsSvIsM. ARcMquFirsaitgio=n16T6im.0eV(CmIiDn@) 40.0 (399.0 -> 80.0) Well_05.d Linear PFHxS 7 1 NFDHA 1.2 1 1.15 1.1 11.457 1.05 1 8 4-2FTS 0.95 0.9 0.85 9 PFHxA 0.8 0.75 0.7 0.65 0.6 10 PFPeS 0.55 0.5 0.45 11 HFPO-DA 0.4 0.35 0.3 0.25 Branched PFHxS 12 PFHpA 0.2 0.15 11.031 0.1 0.05 13 PFHxS 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 Counts vs. Acquisition Time (min) 14 ADONA TEST CONDITIONS Temperature: 44 °C 15 6-2FTS Analytical Column: HALO® PFAS, 2.7 µm, 2.1 x 100 mm Detection: -ESI MRM Data courtesy of STRIDE Center for PFAS Solutions Delay Column: HALO® PFAS Delay, 2.7 µm, 3.0 x 50 mm Injection Volume: 2.0 µL Mobile Phase A: 20 mM Ammonium Acetete Sample Solvent: Methanol (96%) / Water (4%) Mobile Phase B: Methanol LC System: Agilent Triple Quadrupole LC/MS 6400 Gradient: 20-90 %B in 15 min.; hold @ 90 %B for 5 min. Flow Rate: 0.4 mL/min Pressure: 505 bar |4     fused-core.com

HALO® PFAS DELAY COLUMN HALO® PFAS Delay is an application assured column solution. The delay column is used to prevent background PFAS contamination from interfering with the PFAS of interest that are separated with the analytical column. The delay column bonded phase is a highly retentive endcapped silane chosen for its ability to demonstrate delay of background instrument PFAS contamination across multiple mobile phase conditions. For this reason, the HALO® PFAS Delay column is placed upstream of the sample injector. Sample Injector Pump HALO® Solvent Reservoir PFAS Delay HALO® PFAS Data Processing/ Mixer Column Analytical Column System Detector/ Tandem Mass Spectrometer DEMONSTRATION OF THE HALO® PFAS DELAY COLUMN Demonstration of the delay column utility for PFOA extracted ion. The prevalence of PFOA is commonly observed as an instrument materials contaminant- therefore is important to be separated from sample containing PFOA for accurate quantitation. x10 4 -ESI TIC MRM Frag=166.0V (** -> **) Std_01.d 1 2.1 1 2 1.9 1.8 1.7 1.6 1.5 1.4 1.3 1.2 1.1 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 5.654 0 x10 4 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 1.2 1 6 6.5 7 7.5 8 8.5 9 PFOA from sample Counts vs. Acquisition Time (min) 1.15 1.1 1.05 1 0.95 0.9 0.85 TEST CONDITIONS 0.8 PFOA from LC system Analytical Column: HALO® PFAS, 2.7 μm, 2.1 x 100 mm 0.75 Delay Column: HALO® PFAS Delay, 2.7 μm, 3.0 x 50 mm Mobile Phase A: 20 mM Ammonium Acetete 0.7 Mobile Phase B: Methanol Gradient: 20-90 %B in 6 min.; hold @ 90 %B for 2 min. Pressure: 505 0b0.6.a65r Flow Rate: 0.4 mL/min DTeemtepcetrioantu: r-eE:S004I0..544.M555 °RCM Injection Volum0.e4: 2.0 μL Sample Solven0t0.3.:35Methanol (96%) / Water (4%) LC System: Ag0i.2le5 nt Triple Quadrupole LC/MS 6400 0.2 Data courtesy of STRIDE Center for PFAS Solutions 0.15 6.273 0.1 0.05 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 8 8.5 9   |  5

HALO® PAH HALO® PAH The HALO® PAH is an un-endcapped trifunctional APPLICATIONS C18 bonded phase with proprietary manufacturing • EPA 610 process designed on proven Fused-Core® • EPA 8310 +2 technology to provide a fast, efficient separation • EU 15+1 of PAH compounds. • Emerging PAHs The HALO® PAH delivers a method-specific, robust, high efficiency separation of 16 standard PAH compounds with a resolution value of at least 1.5 in under 5 minutes for EPA 8310 and EPA 610. • Application-assured through method qualified lot analysis • Optimal 2.7 µm Fused-Core® particle for rugged, reliable performance delivering high efficiency, low back pressure separations • Well suited for UV, Fluorescence and MS detection • Pressure limit: 600 bar/9000 psi Rapid LCMS analysis of PAH compounds using HALO® PAH PEAK # COMPOUND 1 Naphthalene 2 Acenaphthylene 3 1-Methylnaphthalene 4 2-Methylnaphthalene 5 Acenaphthene 6 Fluorene 7 Phenanthrene 8 Anthracene 9 Fluoranthene 10 Pyrene 11 Benzo[a]anthracene 12 Chrysene Time (min) 13 Benzo[b]fluoranthene Sample Solvent: Methanol 14 Benzo[k]fluoranthene LC System: Shimadzu Nexera TEST CONDITIONS Mobile Phase A: Water/0.1% formic acid 15 Benzo[a]pyrene Column: HALO 90 Å PAH, 2.7 µm, 2.1 x 100 mm Mobile Phase B: Acetonitrile/0.1% formic acid Flow Rate: 0.4 mL/min Gradient: 40-100 %B in 5.0 min; hold @ 100 %B for 3 min 16 Dibenzo[a,h]anthracene Pressure: 289 bar Column Temperature: 30 °C 17 Benzo[ghi]perylene Injection Volume: 1 µL 18 Indeno[1,2,3-cd]pyrene |6     fused-core.com

HALO® PAH COMPETITIVE ADVANTAGE HALO® PAH outperforms a fully porous particle (FPP) 1.8 μm, 95 Å column for a fast 5 min separation of method EPA 8310 + 2 demonstrating improved speed and resolution. PEAK # COMPOUND 1 Naphthalene Absorbance (mAU) 2 Acenaphthylene 3 1-Methylnaphthalene 4 2-Methylnaphthalene 5 Acenaphthene 6 Fluorene 7 Phenanthrene 8 Anthracene 9 Fluoranthene Time (min) 10 Pyrene 11 Benzo[a]anthracene 12 Chrysene 13 Benzo[b]fluoranthene TEST CONDITIONS 14 Benzo[k]fluoranthene Column: HALO 90 Å PAH, 2.7 μm, 4.6 x 50 mm Competitor Column: FPP 95 Å PAH, 1.8 μm, 4.6 x 50 mm HALO® Back Pressure: 256 bar 15 Benzo[a]pyrene Mobile Phase A: Water Competitor Back Pressure: 344 bar Mobile Phase B: Acetonitrile Temperature: 30 °C 16 Dibenzo[a,h]anthracene Gradient: 50-100 %B in 4 min; hold @ 100 %B for 1 min. Detection: 280 nm Flow Rate: 1.8 mL/min Injection Volume: 2 μL 17 Benzo[ghi]perylene 18 Indeno[1,2,3-cd]pyrene SEPARATION OF EU 15 + 1 USING HALO® PAH The separation is completed on a 4.6 x 50 mm HALO® PAH column in less than 8 minutes with excellent resolution between the critical pairs 4 and 5 which only differ by the presence of a methyl group in this EU 15 + 1 separation. 11 PEAK # COMPOUND 1 Benzo[c]fluorene AbAsbosrobrabannccee (@m2A9U)2 nm 2 Cyclopenta[cd]pyrene 3 Benzo[a]anthracene 4 Chrysene 5 5-Methylchrysene 89 6 Benzo[j]fluoranthene 3 7 10 12 14 7 Benzo[b]fluoranthene 13 8 Benzo[k]fluoranthene 26 15 16 9 Benzo[a]pyrene 1 45 10 Dibenzo[a,l]pyrene 11 Dibenz[a,h]anthracene 0.0 2.0 4.0 6.0 8.0 10.0 12 Benzo[ghi]perylene TEST CONDITIONS TiTmimee, m(miinn) 13 Indeno[1,2,3-cd]pyrene Column: HALO 90 Å PAH, 2.7 µm, 4.6 x 50 mm Mobile Phase A: Water Temperature: 30 °C 14 Dibenzo[a,e]pyrene Mobile Phase B: Acetonitrile Detection: 292 nm Gradient: 50-100% B in 4 min.; hold @ 100% for 6 min Injection Volume: 10 µL 15 Dibenzo[a,i]pyrene Flow Rate: 1.8 mL/min Data Rate: 100 Hz LC System: Shimadzu Nexera X2 16 Dibenzo[a,h]pyrene   |  7

ENVIROCLASS SOLUTIONS For the protection of human and environmental health, increased research and regulations of chemicals continue to drive more challenging separation and detection demands. HALO® provides a portfolio of selectivities and particle sizes designed for analysis of small molecules of interest to environmental scientists. Whether your research is driven by HPLC, UHPLC, or LCMS, HALO® offers a tailored solution for your demands. MYCOTOXINS: toxins produced by molds (fungi) and can accumulate in crops, where they pose health hazards to humans and animals. PESTICIDES: any substance used to kill, repel, or control certain forms of plant or animal life that are considered to be pests. HERBICIDES: a broad class of pesticides that are used to remove nuisance plants. CARBONYL COMPOUNDS: significant source of organic carbon balance in both aquatic and atmospheric oxidation processes of hydrocarbons often from vehicle emissions and industrial plants. |8     fused-core.com

MYCOTOXINS The 2 µm HALO® PFP is an ideal choice for high throughput LCMS analysis of mycotoxins, in which multiple isobaric species separation is needed. Here 24 compounds are separated in 5.5 minutes. PEAK # COMPOUND 1 Nivalenol 2 Deoxynivalenol 3 Deoxynivalenol-3-glu- coside 4 Fusarenon X 5 Neosolaniol 6 15-Acetyldeoxyniva- lenol 7 3-Acetyldeoxynivalenol 8 Gliotoxin Time (min) 9 Aflatoxin G2 10 Aflatoxin M1 TEST CONDITIONS Gradient: TIME % B Flow Rate: 0.4 mL/min 11 Aflatoxin G1 Column: HALO 90 Å PFP, 2 µm, 2 x 50 mm 0.01 15 Initial Pressure: 485 bar 12 Aflatoxin B2 Mobile Phase A: Water/2mM ammonium 1.0 25 Temperature: 40 °C 13 HT-2 + Na formate/0.1% Formic acid 2.0 40 Injection Volume: 1 μL 14 Diacetoxyscirpenol Mobile Phase B: Methanol/2mM ammonium Sample Solvent: 95/5 water/methanol 15 Aflatoxin B1 formate/0.1% Formic acid LC System: Shimadzu Nexera X2 16 Ochratoxin A Detection: +ESI MS/MS 17 T-2 +Na 18 Ochratoxin B 19 Citrinin 20 Zearalenone 21 Patulin +MEOH 22 Fumonisin B1 23 Fumonisin B3 24 Fumonisin B2 2.50 41 4.50 100 5.50 100 5.51 15 6.50 Finished   |  9

PESTICIDES High resolution LCMS separation of pesticides using HALO® Biphenyl, which offers 100% aqueous compatibility, ideal to enhance retention of the early eluting polar pesticides. PEAK # COMPOUND PEAK # COMPOUND 1 Daminozide 17 Piperonyl 2 Flonicamid butoxide 3 Thiamethoxam 18 Pyrethrin I 4 Imidacloprid 19 Etoxazole 5 Paclobutrazol 20 6 Fenhexamid 21 Abamectin A 7 Myclobutanil 22 Cypermethrin 8 Bifenazate 23 9 Dimethomorph Bifenthrin Acequinocyl 10 Isomer 1 11 Spirotetramat Fludioxonil Dimethomorph (observed in negative ion 12 mode) 13 Isomer 2 Time (min) 14 Spinosad A 15 Spinosad D TEST CONDITIONS Flow Rate: 0.2 mL/min 16 Trifloxystrobin Column: HALO 90 Å Biphenyl, 2.7 μm, 2.1 x 100 mm Pressure: 89 bar (initial) Spinetoram Temperature: 40 °C Pyrethrin II A=Water/0.1% formic acid/4 mM ammonium formate Injection Volume: 1 μL B=Acetonitrile/0.1% formic acid/4 mM ammonium Sample Solvent: acetonitrile Detection: +ESI formate Gradient: 0-15% B in 1.01 min; 15-35% B in 3.99 min; 35-62% B in 1 min; 62-100% B in 25 min; hold at 100% B for 4 min 11 triazine pesticides are separated in less than 8 minutes using a HALO® AQ-C18 column for its symmetrical peak shape and high resolution. Absorbance (mAU) PEAK # COMPOUND 1 Time (min) 2 Acetone (solvent) 3 Atraton TEST CONDITIONS 4 Column: HALO AQ-C18, 2.7 μm, 4.6 x 150 mm 5 Prometon Mobile Phase A: 0.02 M sodium phosphate buffer, pH=3.0 6 Simazine Mobile Phase B: Acetonitrile 7 Simetryn Gradient: hold at 40% B for 8 min; 40-75% B in 2 min 8 Atrazine Flow Rate: 1.6 mL/min. 9 Ametryn 10 Propazine 11 Prometryn Terbutryn Terbuthylazine Pressure: 310 bar at start Temperature: 35°C Detection: UV 254 nm, VWD Injection Volume: 2.0 μL Sample Solvent: 25/75: acetone/acetonitrile |10     fused-core.com

HERBICIDES EPA Method Absorbance (mAU) PEAK # COMPOUND 549.2 specifies 1 Diquat dibromide the use of 2 Paraquat dichloride two different wavelengths TEST CONDITIONS to limit Column: HALO 90 Å Phenyl-Hexyl, 5 μm potential matrix interferences. 3.0 x 100 mm The separation Mobile Phase: 13.5 mL orthophosphoric acid, of diquat and paraquat is 10.3 mL diethylamine and 3.0 g complete in of hexane-sulfonic acid, sodium salt less than 1.5 in 1 L of water minutes on a Flow Rate: 1.0 mL/min HALO® Phenyl- Pressure: 156 bar Hexyl column Temperature: 30 °C using an ion- Detection: UV 257, 308 nm, VWD pair containing Injection Volume: 40 μL mobile phase. Sample Solvent: Water Time (min) CARBONYL COMPOUNDS By using a HALO® Absorbance (mAU) PEAK # COMPOUND C18 column 1 with a ACN/ Time (min) 2 Formaldehyde-2,4-DNPH THF-containing 3 mobile phase, the Flow Rate: 1.5 mL/min 4 Acetaldehyde-2,4-DNPH DNPH-derivatized Pressure: 355 bar 5 carbonyl Temperature: 30 °C 6 Acetone-2,4-DNPH compounds are Detection: UV 360 nm, VWD 7 fully resolved from Injection Volume: 0.3 μL 8 Acrolein-2,4-DNPH their isomers with Sample Solvent: Acetonitrile 9 high resolution. 10 Propionaldehyde-2,4-DNPH 11 TEST CONDITIONS 12 Crotonaldehyde-2,4-DNPH Column: HALO 90 Å C18, 2.7 μm, 4.6 x 150 mm 13 Mobile Phase: 55/45 - A/B 2-Butanone-2,4-DNPH A: Water Methacrolein-2,4-DNPH B: Acetonitrile/THF (80/20) Gradient: 45-58% B in 7.5 min; 58-80% B in Butyraldehyde-2,4-DNPH 1.5 min; hold at 80% B for 3 min Benzaldehyde-2,4-DNPH Valeraldehyde-2,4-DNPH m-Tolualdehyde-2,4-DNPH Hexaldehyde-2,4-DNPH 2,4-DNPH = 2,4-Dinitro- phenylhydrazone i = anti, syn, isomers of the respective DPNH derivatives |    11

HALO® ENVIROCLASS SPECIFICATIONS TABLES SPECIFICATIONS BONDED PHASE PARTICLE SIZE (µm) SURFACE AREA LOW pH/T HIGH pH/T ENDCAPPED (m2/g) LIMIT LIMIT PFAS Analytical 2.7 135 2/60 °C 9/40 °C Yes PFAS 2.7 90 2/60 °C 9/40 °C Yes Delay PAH 2.7 135 2/60 °C 9/40°C No ORDERING INFORMATION DELAY COLUMNS ANALYTICAL COLUMNS PFAS Dimensions: ID x Length (in mm) PN Dimensions: ID x Length (in mm) PN 2.1 x 50 92812-413 3.0 x 50 92113-415 2.1 x 100 92812-613 4.6 x 50 92114-415 2.1 x 150 92812-713 2.1 x 250 92812-913 3.0 x 50 92813-413 3.0 x 100 92813-613 3.0 x 150 92813-713 3.0 x 250 92813-913 ANALYTICAL COLUMNS GUARD COLUMNS 2.7 µm Guard Columns 3-pk Dimensions: ID x Length (in mm) PN Dimensions: ID x Length (in mm) PN 2.1 x 50 92842-412 2.1 x 100 92842-612 2.1 x 5 92842-112 2.1 x 150 92842-712 3.0 x 50 92843-412 3.0 x 5 92843-112 3.0 x 100 92843-612 PAH 3.0 x 150 92843-712 4.6 x 5 92844-112 4.6 x 50 92844-412 4.6 x 100 92844-612 Guard Column Holder 94900-001 4.6 x 150 92844-712 |12     fused-core.com

OTHER SMALL MOLECULE HALO® OFFERINGS FOR ENVIRONMENTAL APPLICATIONS Below is a list of other phases that accomplish complex separations for environmental needs as well as many other applications of interest for small molecule separations. SMALL MOLECULE BONDED PHASE FEATURES AND BENEFITS AQ-C18 • Ideal for mixtures of polar and non-polar solutes C18 • High retentivity of C18 with alternate selectivity HILIC • 100% aqueous compatible • Universal phase for acids, bases and neutral solutes • Excellent stability at low to mid mobile phase pH • Wide range of published literature applications • Ideal for polar analytes • Alternate mode to reversed-phase modes • Can be used in HILIC and normal-phase modes • Ideal for broad range of analytes C8 • Less hydrophobic (less retentive) than C18 • Better ion-pair applications than C18 ES-CN • Ideal for polar analytes • Alternate selectivity to alkyl phases • 100% aqueous compatible PHENYL-HEXYL • Ideal for separating aromatic compounds using pi-pi interactions • Alternate selectivity to alkyl phases • Also available with 160 Å pores for peptide mapping BIPHENYL • Ideal for aromatic (pi-pi) compounds • Alternate selectivity to alkyl phases • 100% aqueous compatible PFP • Ideal for aromatics and electron-rich compounds RP-AMIDE • Alternate selectivity to alkyl phases PENTA-HILIC • Useful in RPLC and HILIC modes • Ideal for basic compounds (alcohols, acids, phenols, catechins) • Alternate selectivity to alkyl phases • 100% aqueous compatible • Ideal for polar compounds poorly retained in RPLC • Alternate selectivity for HILIC mode • Excellent peak shape for basic compounds in HILIC mode C30 • Ideal for hydrophobic, long chain, structurally related isomers • Alternate alkyl phase with high shape selectivity • 100% aqueous compatible |    13

|A1M4T 2 0_01_ENVIROCLASS fused-core.com HALO and Fused-Core are registwewrewd.fturasdeedm-caorrkes.coofmAdvanced Materials Technology, Inc.


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