Forte GC columns

Customer Service About Canadian Life Science Au suject de Canadian Life Science Founded in 1997, Canadian Life Science (CLS) has steadily grown Fondée en 1997, Canadian Life Science (CLS) n’a cessé de croître to Canada’s leading supplier of LC/GC columns, chromatography pour devenir un chef de file canadien dans la vente de colonnes and dissolution accessories as well as 2d long-term sample HPLC / GC, d’accessoires de chromatographie et dissolution, storage tubes and racks for bio-banking and compound ainsi que dans la vente de tubes et systèmes d’entreposage long management. terme pour échantillons. For over 22 years, CLS has provided customers with unparalleled Depuis plus de 22 ans, CLS offre à ses clients un service et service, expertise and a wide selection of quality and cost- une expertise inégalés, en plus d’un large éventail de produits effective products that offer maximum flexibility to solve your économiques de qualité offrant une flexibilité maximale afin de analytical challenges. CLS is your foundation for what matters. résoudre vos problèmes analytique. CLS est votre pilier pour ce qui compte. Everyone is accessible to you Nous sommes très accessible We believe that our customers like our personal touch. Nous avons une approche personnelle et nous répondons à tous We take your calls personally, not a computer, not voicemail . les appels lorsque vous téléphonez au bureau chef. Nous n’avons We have dedicated inside customer service staff who care, and pas de boîte vocale. take care for specific areas. We have knowledgeable outside staff Notre personnel est dévoué au service à la clientèle et ce à based in most of the larger cities accross Canada. travers le Canada. Si vous désirez parler directement à notre We supply world class products Directrice de comptes au Québec, vous pouvez contacter Canadian Life Science distributes for top-quality manufacturers. Genevieve Lemieux au 514.428.8034. Our product offering has been chosen in a way that will give you Nous choisissons les meilleurs produits au monde the maximum flexibility solving your analytical challenges. En tant que distributeur nous avons l’avantage de choisir les Technical Support meilleurs fournisseurs et notre gamme complète de produit vous Our technical staff is happy to respond to customer enquiries. donne l’embarras du choix. We can also supply extensive application information from one Support Technique of the world’s largest data bases. Notre équipe technique se fait toujours un plaisir de vous aider à Plan the best delivery faire le meilleur choix pour vos applications. When you decide to purchase a product from Canadian Life Service et logistique Science our customer service department will find the fastest Lorsque vous placez une commande chez nous, nous nous and most economical way to send your order to you. For assurons de choisir le moyen le plus efficace et économique your convenience we have two warehouse locations, one in d’acheminer la marchandise. Edmonton, AB and one in Peterborough, ON. Nous avons deux entrepôts dont un en Alberta pour servir nos We’re glad to help clients de l’Ouest et un en Ontario pour servir nos clients de l’Est. We are never too busy to take your call. C’est un plaisir de vous servir Try us. You’ll notice the difference. Nous ne sommes jamais trop occupés pour vous répondre. Canadian Life Science, friendly people to do business with! Essayez-nous et vous verrez la différence. Il est agréable de faire affaire avec nous! I-22 CANADIAN Proud member of the Chrom4 buying group Fier membre du groupe dʼacheteur Chrom4 LIFE SCIENCE 1 888-226-2775 :: [email protected] :: www.lifescience.ca

SGE Capillary Columns SGE Analytical Science Few companies can claim to be able to provide a total solution for analytical science; SGE is one of those few. From sample preparation, to injection, the separation and throughout the chromatographic process in either LC or GC there is a SGE product that has been designed to provide you with the results you need time and again. This brochure highlights some of SGE’s capabilities in capillary gas chromatography, specifically in the technically demanding area of capillary columns. SGE columns are engineered for consistent separation performance and can be found in many laboratories being tasked to perform everything from routine analyses to the latest R&D challenges. forte™ GC Capillary Columns With over 30 year’s experience, GC capillary columns truly are SGE’s strength, its forte. SGE is the only independent manufacturer of GC capillary columns that has the skill and technology to control all critical processes from producing the fused silica capillary tubing, through the phase synthesis, to the validation of the performance of each column. SGE offers a comprehensive range of forte™ GC capillary columns for almost any chromatographic application providing the best possible combination of, Performance, Robustness, Reproducibility, Low Bleed and Inertness. With this brochure we bring to you a small taste of the applications you might consider running on an SGE GC capillary column. If the application is not in this guide, please visit our website www.sge.com. For further information on our full range of products, please visit www.sge.com

Contents Guidelines for Choosing Columns 1-2 Recommended Column by Application 3 Environmental 4-12 Food, Flavors and Fragrances 13-18 Fuels and Petrochemical 19-22 Chemical 23-27 Pharmaceutical 28-32 33-36 Forensic SGE Capillary Column 37-39 Part Number Listing 39 GC Column Replacement Guide GC Troubleshooting and Maintenance 40 For further information on our full range of products, please visit www.sge.com

Guidelines for Choosing Columns 1 Stationary Phase Components • S elect the least polar phase that will perform the 2 separation you require 1 3 45 1. Benzene 2. Toluene • N on-polar stationary phases separate analytes 3. Ethylbenzene predominantly by order of boiling point. 6 4. p-Xylene • Increase the amount of phenyl and/or cyanopropyl 5. m-Xylene content in the phase, and the separation is then 6. o-Xylene influenced more by differences in dipole moments or charge distributions (BP10 (1701), BPX35, 1 SolGel-WAX™ BPX50, BP225 and BPX70) 2 Polar • To separate compounds that differ more in their 3 4, 5 hydrogen bonding capacities (for example aldehydes and alcohols), polythylene glycol type phases are 6 SolGel-1ms™ best suited - SolGel-WAX™, BP20 (WAX) and BP21 Non-Polar (FFAP) 0.5 4.0 min Effect of Stationary Phase. BTEX analysis on a polar (SolGel-WAX™) column and a 100% dimethyl polysiloxane (SolGel-1ms™), both 30 m x 0.25 mm ID X 0.25 µm film. Stationary Phase Polarity Van der Waals BP1, BPX1, SolGel-1ms BPX5 HT8 HT5 BPX35 BPX-Volatiles BPX50 BPX70 BP225 BP21 Cydex-B BP20 SolGel Wax �-bonding H-bonding BPX90 Dimethyl polysiloxane Phenyl Polysilphenylene siloxane Polycarborane siloxane Cyanopropylphenyl siloxane Cyanopropyl Polysilphenylene siloxane Polyethylene glycol 2 Internal Diameter • The smaller the diameter the greater NORMAL - 0.25 mm ID FAST - 0.10 mm ID the efficiency, hence better resolution. Fast columns (0.1 mm ID) are used for faster Chromatogram using a conventional Chromatogram using a FAST analysis because the same resolution can be achieved in a shorter time. (30 m x 0.25 mm ID) BPX5 column with (10 m x 0.1 mm ID) BPX5 column 1 23 4 a 0.25 µm film. 2 3 4 with a 0.1 µm film. 5 5 6 7 8 9 10 1 6 78 10 12 9 11 13 11 15 12 13 16 14 14 15 16 0 min 26 0 min 12 CoCommppoonneenntsts 5. Phenanthrene 9. Benzo(a)anthracene 13. Benzo(a)pyrene 6. Anthracene 10. Chrysene 14. Indeno(1,2,3,-c,d)pyrene 1. Naphthalene 7. Fluoranthene 11. Benzo(b)fluoranthene 15. Dibenzo(a,h)anthracene 2. Acenaphthylene 8. Pyrene 12. Benzo(k)fluoranthene 16. Benzo(g,h,i)perylene 3. Ethylbenzene 4. Fluorene Effect of Internal Diameter. Polynuclear Aromatic Hydrocarbon (PAH) analysis. 1 For further information on our full range of products, please visit www.sge.com

3 Column Length R = 2.05 • Always try to select the shortest column that will provide the required resolution for the application. If the maximum column length 3.7 min 15 metres available is being used, and resolution of the sample mixture is still inadequate then try changing the stationary phase or internal diameter. R = 2.91 • Resolution is proportional to the square root of the column 7.5 min efficiency. Therefore, doubling the column length will only increase the resolving power of the column by approximately 40%. 0 30 metres Effect of Length R = 4.15 15.3 min 60 metres 20 min 4 Film thickness R (Resolution) = 3.14 • F or samples with a variation in solute concentration, a thicker film column is recommended. This will reduce the possibility of broad overloaded peaks 4.7 min 0.25 µm co-eluting with other compounds of interest. If the separation of two solutes is sufficient and co-elution is still unlikely, even with large differences in 7.4 min R = 3.60 concentration, then a thinner film can be used. The greater the film thickness the greater the retention of solutes, therefore the higher the elution 0.50 µm temperature. As a rule, doubling the film thickness results in an increase in elution temperature of approximately 15-20° under isothermal conditions. R = 4.96 Using a temperature program, the increase in elution temperature is slightly less. 13.2 min 1.0 µm 0 20 min Effect of Film Thickness. PHASE RATIO ß= —4id—df— Film thickness 0.1 0.15 Column ID (mm) 0.32 0.53 where (µm) 250 - 0.22 0.25 800 1325 ß = ratio 0.10 - 550 625 id = column internal diameter (µm) 0.15 - 250 -- - 883 df = film thickness (µm) 0.25 - 150 220 250 320 530 0.50 - 110 125 160 265 Formula to calculate Phase Ratio 1.00 - 75 55 63 132 3.00 - - -- 80 • From the phase ratio value ß, a column can be categorized for the type of 5.00 - -- 27 44 application it would best suit. The smaller the ß value, the greater the ratio - 16 26 of phase to the column inner diameter, making it better suited for analyzing volatile compounds. Columns which have thin films are generally better Above shows the phase ratio (ß) available for the SGE range of capillary suited for high molecular weight compounds and are characterized by large ß columns. Keeping a similar phase ratio when changing column internal values. diameters will ensure that your chromatographic parameters will not need substantial changes. For further information on our full range of products, please visit www.sge.com 2

Recommended Column by Application PHASE Application SolGel-1ms BPX1 BPX5 HT5 HT8 BPX35 BPX-Volatiles BP10 BPX50 SolGel-WAX™ BP21 BPX70 CYDEX-B BPX90 BP1 Acidic/Neutral Drugs BP1-PONA BP5 BP624 BP20 Acids Alcohols Amines Aliphatic Amines Aromatic Antidepressants Aromatic -PAH Aroclors Beverages -Alcohols Butter-Fat Chiral - Componds Chlorinated Aromatics Cigarette Lighter Fuel Dioxins Essential Oils Food - FAME Glucose - Methylated Herbicides Industrial Solvents Ketones Monomers Nitroaromatics Organochlorine Pesticides Organophosphorous Pesticides Paraffins PCB’s Petroleum Phenols Phthalates Plant Sterols Polymers Polywax Pyrethroids Racehorse Doping Mixture Sedatives Semivolatiles Silicon Oil Solvents Sugars-Alditol Acetates Triglycerides TRPH Volatiles Xylenes Indicates recommended phases to be used for the application Indicates alternative phases that can be used for the application 3 For further information on our full range of products, please visit www.sge.com

Environmental The widespread use of GC-MS for Environmental analyses requires both low bleed and inertness. The broad range of compounds of interest means that medium polarity phases become more useful. BPX5, BPX35, and BPX50 provide a range of polarities, all with low bleed, high temperature limits, and robustness. For specialized applications such as PCBs, SGE’s HT8 delivers unique separation capabilities. Applications • Analysis of PCB'S, PCDT'S, and other complex mixtures using BPX5 and SolGel-1ms • HT8: The Perfect PCB Column • Fast Pesticide Screening Using a BPX5 GC Capillary Column • Analysis of Polychlorinated Dibenzodioxins and Furans on BPX5 • Analysis of Polynuclear Aromatic Hydrocarbons on BPX35 • Analysis of Polynuclear Aromatic Hydrocarbons on BPX5 • Analysis of Volatile Organic Pollutants on BPX-Volatiles • Applications using BPX90 • Analysis of Volatiles in Drinking Water on 25 m BP624 Column • Polychlorinated Biphenyls PCB Analysis For further information on our full range of products, please visit www.sge.com 4

Environmental ANALYSIS OF PCB'S, PCDT'S, AND OTHER COMPLEX MIXTURES USING BPX5 AND SOLGEL-1ms Mono- Di- Tri- Tetra- Penta- Hexa- Hepta- Octa- Nona- Deca- 37 77 100 154 138 183 203 206 209 100 2 3 11/8 15 1213 35 202807 85/155 47 126 190 153 181 75 1 28 119 SIM 7-Group Analysis of 44 mono- to deca-PBDE's via 10 17 49 99 cool-on-column (3-µL). Deca-PBDE 209 at 310 °C, PBDEs 33/28 and 138/166 7 32 66 partially resolved, PBDE 85 coeluted with 155. 30 71 118 166 - 44 Mono-Deca-PBDEs - 6890 GC/Autospec HRMS % 2533 - Cool-on-column inlet %% - (No liner but 0.53 mm retention gap) 116 12.5 m BPX5 0.15 mm ID 0.1 µm EXPERT TIP - 0.25 m 0.53 mm ID plus 0 Time - 2 m 0.25 mm retention gaps 5.00 7.50 10.00 12.50 15.00 17.50 20.00 22.50 25.00 27.50 30.00 32.50 35.00 37.50 40.00 42.50 45.00 47.50 50.00 52.50 55.00 - He programmed 245-415 kPa - 150 ºC-315 ºC @ 3 ºC/min Tetra- Penta- Hexa- Hepta- Octa- SIM 2-Group Analysis for non-o-PCBs. Tetra-PCB's 77 and 81 (A) are resolved FF D D F DF OCDD OCDF from residual o-PCB's (97, 87, 110, and 136). Penta-126 (B), and hexa-169 100 2378-D F FD D (C) also detected in eluate 2-basic alumina of DX-3 QC sediment extract (5 F D of 500 µL) Ion chromatograms (not smoothed). PBDE's are in same eluate 2378-F F (Peterman et al., 2006). Tetra-PBDE 47 from (M-2 Br)+ incidentally detected (B); 0.3 ng/g near lab background. 0 16.00 18.00 20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00 46.00 48.00 50.00 Time - Dioxin-like non-ortho PCBs Tri- Tetra- Penta- Hexa- Hepta- Octa- - HP 5890A GC/VG 70S HRMS - Heated (275 ºC) Direct inlet 100 - 4 mm Siltek Cyclo-Uniliner - 30 m SolGel-1ms 0.15 mm ID 0.1 µm OCDT - 2.5 m x 0.25 mm ID retention gap - He constant at 415 kPa - 155 ºC (1 min) - 205 ºC @ 1.8 ºC/min then to 310 ºC @ 3.6 ºC/min ACKNOWLEDGEMENT Paul H. Peterman, US Geological Survey, Columbia Environmental Research Center. For additional information see SGE application note AN-0030-C. 0 16.00 18.00 20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00 46.00 48.00 50.00 Time SGE liners undergo a high temperature deactivation process making them ideal for using with active compounds 5 For further information on our full range of products, please visit www.sge.com

HT8: THE PERFECT PCB COLUMN Environmental Separation of CB31 and CB28 Chromatogram on the left demonstrates clearly the significant difference in selectivity of the HT8 column. By GC/MS, quantitation of CB28 using a HT8 Standard 5% Phenylsiloxane standard 5% phenylpolysiloxane column is impossible as coelution with CB31 (with the same number of chlorines) occurs. 28 HT8 separates the two congeners by a full minute allowing quantitation to be performed with ease. 31 31/28 28/53 AROCLOR 1242 HT8, 0.25 µm film 31 Phase: 50 m x 0.22 mm ID Column: 80 °C, 2 min 18 Initial Temp: 30 °C/min 20/33/45 Rate 1: 170 °C Temp 2: 3 °C/min 8 44 70 Rate 2: Split, 300 °C 17 16 52/69 66 Final Temp: He, 40 psi 22 49/43 Carrier Gas: ECD, 330 °C Detector: 74 60/113 56 47/48/65 32 25 101/150 15 26/50 97/117/86 115/87 27 77/149 107/108/134 51 95 110 118 29 40 10 4 6 19 99 85 97 84 38 12/24 36 75 59 67 63 76/80 148 81 91/121/155 AROCLOR 1242 Congener # Cl Position Cl # Identification 20 25 minutes 30 35 72/64 by GC/MS 103/71 42 23-24 4 42 41 68/37 96 236-26 5 40 35 34-3 3 96/35 64 235-4 4 72 25-35 4 103 246-25 5 71 26-34 4 41 234-2 4 68 24-35 4 37 34-4 3 100 246-24 5 AROCLOR 1260 153/122 174/162/128 180 170 209 Int.Std. 179/132 Column and Oven conditions 77/149 138 are as listed for Aroclor 1242 151 101/150 177 190/203 194 199 AROCLOR 1260 187/182 Congener # Cl Position Cl # Identification 138 164/163 by GC/MS 110 197/171 130 234-235 6 136/86 200/172 146 141 178 2356-235 7 183/166 135 156 141 2345-25 6 144 191 97/117 195 115/87 206 164 236-345 6 107/108/134 205 118 163 2356-34 6 176 84 207 138 234-245 6 92 208 173 189 178 160 23456-3 6 35 130 175 2346-235 7 105 131 204 185/159 158 2346-34 6 40 45 129 minutes 50 55 187 2356-245 7 182 2345-246 7 129 2345-23 6 137 For further information on our full range of products, please visit www.sge.com 6

Environmental FAST PESTICIDE SCREENING USING A BPX5 GC CAPILLARY COLUMN 7e+006 6e+006 5e+006 4e+006 3e+006 2e+006 1e+006 ____ TIC 0 300 400 500 600 700 800 900 1000 ACKNOWLEDGEMENT 348 1048 1748 2449 3149 3849 4550 5250 SGE would like to thank Prof Time (s) Jana Hajšlová and Jakub Schurek Spectrum # from VSCHT (Prague, CZ) for providing these chromatograms. 7e+006 Phenol 3-phenoxy- Primicarb Phosphionthiotic amide, P,P-diphenyl- Cycohexene 1,3,4,5,6 pentachloro-, s Mefenoxam Carbaril Fenthion Aldrine Clofenvifos Captan Imazali p,p’-DDT Fenoxycarb Dimpylate Malathion Quinalphos 2-Methylphenothiazine Endosulfan sulfate 6e+006 Chlorpyrifos Phosphamidon Mitotane Btethrin Cyclohexane 1,2,3,4,5,6 hexachloro Dieldrin 5e+006 Ethion Mitotane Trizaophos Octyl adipate 4e+006 3e+006 2e+006 1e+006 0 520 540 560 580 600 620 640 660 680 700 Time (s) Phase: SGE forte BPX5DX Column: 40 m x 0.18 mm ID x 0.18 μm Initial Temp: Rate 1: 80 ºC, 1 min Final Temp: 25 ºC/min to 300 ºC Detector Type: 300 ºC, 7 min Carrier Gas: LECO™ TOF Mass spectrometer Carrier Gas Flow: He Average Linear Velocity: constant flow mode at 1 mL/min Injection Mode: ~75 cm/sec Injection Temperature: splitless Total run time: 270 ºC Detector voltage: 16.8 min Data acquisition speed: 1700 V Column Part Number: 10 Hz 054229 7 For further information on our full range of products, please visit www.sge.com

Environmental Peak Name R.T. (s) Peak Name R.T. (s) number number Methamidophos 341.292 Penconazole 585.765 1 Dichlorovos 342.291 50 Tolylfluanide 587.622 2 Phosphorodithioic acid, O,O-diethyl ester 381.561 51 Quinalphos 590.478 3 Mevinphos 404.552 52 Procymidone 592.191 4 Carbamic acid, phenyl-, 1-methylethyl ester 418.261 53 Captan 599.902 5 Methacrifos 54 Methidathion 601.33 6 Benzene, (3-chloro-1-propenyl) 427.4 55 o,p'-DDE 602.044 7 Butyl dimethyl phosphate 433.969 56 Folpet 602.901 8 1-Naphthalenol 436.539 57 Imazalil 611.755 9 Bibenzyl 442.965 58 Bupirimate 614.325 10 Cyclohexene, pentachloro- 445.107 59 2-Methylphenothiazine 616.896 11 Heptenophos 449.534 60 p,p'-DDE 617.895 12 Endosulfan 453.39 61 Mitotane 623.179 13 Omethoate 462.672 62 Dieldrin 628.605 14 Diphenylamine 465.099 63 Ethion 635.46 15 Carbamic acid, (4-chlorophenyl), 1-methylethyl ester 476.095 64 Mitotane 642.314 16 Monocrotophos 479.094 65 m,p'-DDD 643.171 17 Cyclohexane, 1,2,3,4,5,6-hexachloro- 485.091 66 o,p'-DDT 644.17 18 Benzene, hexachloro- 500.228 67 Triazophos 647.455 19 Phenol, 3-phenoxy- 501.799 68 Endosulfan I 648.883 20 Dimethoate 504.512 69 Octyl adipate 651.168 21 Dimpylate 505.083 70 p,p'-DDT 665.019 22 Phosphamidon 509.796 71 Endosulfan sulfate 670.731 23 α-Lindane 514.651 72 Tebuconazole 673.444 24 Benzonitrile, pentachloro- 515.222 73 Bifenthrin 680.013 25 Cyclohexane, 1,2,3,4,5,6-hexachloro- 517.792 74 Fenoxycarb 693.436 26 Etrimfos 518.792 75 Bromopropylate 695.436 27 Pirimicarb 520.22 76 Phosmet 701.576 28 Tetrachloroisophthalonitrile 526.36 77 Endrin ketone 709.43 29 Phosmet 527.217 78 λ-Cyhalothrin 718.141 30 Phosphinothioic amide, P,P-diphenyl- 527.788 79 Tetradifon 721.854 31 Phosphamidon 532.072 80 Phosalone 723.282 32 γ-Lindane 534.786 81 Fenarimol 754.126 33 Cyclohexene, 1,3,4,5,6-pentachloro-, ç- 535.642 82 Azinphos-ethyl 759.124 34 Methyl chlorpyrifos 540.498 83 Permethrin 768.264 35 Mefenoxam 542.497 84 Permethrin 774.975 36 Pirimiphos methyl 547.78 85 Pyridaben 786.114 37 Carbaril 551.065 86 Cyfluthrin 805.534 38 Heptachlor 553.492 87 Cyfluthrin 807.248 39 Malathion 554.92 88 Cypermethrin 819.957 40 Fenitrothion 557.348 89 Cypermethrin 826.383 41 Chlorpyrifos 558.205 90 Cypermethrin 831.238 42 Dichlofluanid 563.346 91 Cypermethrin 833.095 43 Fenthion 563.774 92 Esfenvalerate 895.927 44 Parathion 567.915 93 Fenvalerate α 914.062 45 Triadimefon 94 Difenoconazole 950.476 46 Aldrine 569.2 95 Difenoconazole 956.617 47 cis-Chlorfenvinphos 569.772 96 Deltamethrin 972.182 48 Clofenvinfos 574.341 97 49 577.483 584.194 The list of some pesticides used in the sample, and their retention times For further information on our full range of products, please visit www.sge.com 8

Environmental ANALYSIS OF POLYCHLORINATED DIBENZODIOXINS AND FURANS ON BPX5 Single Ion Chromatograms of TCDF Isomers Phase: BPX5, 0.25 µm 100% Column: 50 m x 0.22 mm ID m/z 305.8987 2,3,7,8 - TCDF Initial Temp: 80 °C Rate 1: 10 °C/min 0 Temp1: 240 °C 100% Rate 2: 2 °C/min Temp 2 : 280 °C m/z 317.9389 13C12 - 2,3,7,8 - TCDF Rate 3: 10 °C/min 0 Final Temp: 320 °C Detector: Mass Spectrometer Column Part Number: 054114 17 18 19 20 21 minutes Single Ion Chromatograms of TCDD Isomers EXPERT TIP 100% m/z 321.8936 2,3,7,8 -TCDD Remember : the lower the temperature, the longer your column will last. 0 100% m/z 333.9339 13C12 - 2,3,7,8 -TCDD 0 18 19 20 21 minutes SGE wishes to acknowledge Dr P.Ambridge, Dr A.Fernandes and C.Brook at AEA Technology, Harwell, U.K. ANALYSIS OF POLYNUCLEAR AROMATIC HYDROCARBONS ON BPX35 13 Components 9. Benzo (a) anthracene 2 1. Naphthalene 10. Chrysene 2. Acenaphthylene 11. Benzo (b) fluoranthene 4 3. Acenaphthene 12. Benzo (k) fluoranthene 4. Fluorene 13. Benzo (a) pyrene 56 7 5. Phenanthrene 14. Indeno (1,2,3, -c,d)pyrene 8 6. Anthracene 15. Dibenzo (a,h) anthracene 7. Pyrene 16. Benzo (g,h,i) perylene 10 12 8. Fluoranthene 9 11 13 BPX35, 0.25 µm Phase: 30 m x 0.22 mm ID 14 Column: 100 °C,1 min 15 Initial Temp: 10 °C/min Rate: 360 °C, 10 min 16 Final Temp: He, 25 psi Carrier Gas: FID 380 °C 0 10 20 30 minutes Detector: 054714 Column Part Number: ANALYSIS OF POLYNUCLEAR AROMATIC HYDROCARBONS ON BPX5 5 Components 9. Chrysene 68 (0.5ng each component) 10. Benzo (a) anthracene 1. Naphtalene 11. Benzo (b) fluoranthene 2. Acenaphtylene 12. Benzo (k) fluoranthene 3. Acentaphthene 13. Benzo (a) pyrene 4. Fluorene 14. Indeno (1,2,3, -c,d)pyrene 5. Phenanthrene 15. Dibenzo (a,h) anthracene 6. Anthracene 16. Benzo (g,h,i) perylene 7. Pyrene 8. Fluoranthene 7 12 9 10 13 Polynuclear Aromatic Hydrocarbons (EPA 625 PAHs) 4 1 23 11 Phase: BPX5, 0.25 µm film 15 Column: 25 m x 0.22 mm ID 16 Initial Temp: 50 °C, 2 min 14 Rate: 8 °C/min Final Temp: 290 °C, 10 min Carrier Gas: HP5971 MSD 5 10 20 minutes 30 40 Detector: Split 40:1 Carrier Gas: He, 15 psi Column Part Number: 054113 9 For further information on our full range of products, please visit www.sge.com

Environmental ANALYSIS OF VOLATILE ORGANIC POLLUTANTS ON BPX-VOLATILES 6 35, 36 1 23 4 5 33 37 38 40 43, 44 46 48 49 51 52 54 32 41, 42 45 47 50 53 55 1.60 2.00 2.40 2.80 3.20 3.60 4.00 4.40 39 14.00 15.00 16.00 17.00 18.00 19.00 20.00 1600000 8 35, 36 59 1200000 16,17 41, 42 43, 44 45 54 57 60 800000 46 49 51 58 400000 40 33 37 38 48 50 53 0 47 52 55 25 32 18 22 34 13 28 11 15 20 27 12 14 19 21 23 24 29 26 30 39 56 9 10 35 7 31 6 2 4 1 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 Chromatogram showing analysis of commonly screened volatile organic pollutants Components 31. 1,2-Dibromethane Phase: BPX-Volatiles 1 µm film 1. Dichlorodifluoromethane 32. Chlorobenzene 502.2 mix: 2. Chloromethane 33. Ethylbenzene Column: 200 ppm in Methanol 3. Vinyl chloride 34. 1,1,2-Dibromochloromethane 4. Bromomethane 35. p-Xylene Initial Temp: 40 m x 0.18 mm ID 5. Chloromethane 36. m-Xylene Rate 1: 6. Trichlorofluoromethane 37. o-Xylene Rate 2: 40 °C , 0 min 7. 1,1-Dichloroethene 38. Styrene Final Temp: 8. Dichloromethane 39. Bromoform Detector Type: 6 °C to 210 °C 9. trans-1,2-Dichloroethene 40. Isopropylbenze Carrier Gas: 10. 1,1-Dichloroethane 41. Bromobenzene Carrier Gas Flow : 15 °C to 240 °C 11. 2,2-Dichloropropane 42. 1,1,2,2-Tetrachlorethane Constant Flow: 12. cis-1,2-Dichloroethene 43. 1,2,3-Trichloropropane Average Linear Velocity: 240 °C, 5 min 13. Bromochloromethane 44. n-Propyl benzene Injection Mode: 14. Chloroform 45. 2-Chlorotoulene Split Ratio: Mass Spectrometer 15. 1,1,1-Trichloroethane 46.1,3,5-Trimethylbenzene Injection Volume: 16. 1,1-Dichloropropene 47. 4-Chlorotoulene Injection Temperature: He, 40.3 psi 17. Carbon tetrachloride 48. tert-Butylbenzene Autosampler: 18. Benzene 49. 1,2,4-Trimethylbenzene Liner Type : 1.2 mL/min 19. 1,2-Dichloroethane 50. sec-Bythlbenzene Liner Part Number: 20. Trichloroethene 51. 1,3-Dichlorobenzene Column Part Number: On 21. 1,2-Dichloropropane 52. p-lsopropyltoulene 22. Dibromomethane 53. 1,2-Dichlorobenzene ms-NoVent™Part No: 35 cm/sec at 40 °C 23. Bromodichloromethane 54. n-Butylbenzene HP5973 restrictor: 24. cis-1,3-Dichloropropene 55. 1,4-Dichlorobenzene Full scan: Split 25. Toluene 56. 1,2-Dibromo-3-chloropropane 26. trans-1,3-Dichloropropene 57. 1,2,4-Trichlorobenzene 50:1 27. 1,1,2-Trichloroethane 58. Hexachlorobutadiene 28. Tetrachloroethene 59. Naphthalene 1 µL 29. 1,3-Dichloropropane 60.1,2,3-Trichlorobenzene 30. Dibromochloromethane 250 °C No 4 mm ID Single Taper Liner 092017 054860 113400 113409 45-450 m/z For further information on our full range of products, please visit www.sge.com 10

Environmental APPLICATIONS USING BPX90 10.0 15. 20.0 4.0 10.0 15.0 HCCH isomers and HCB Separation of aromatics Ronnel and heptachlor ceolute Aldrin and fenthion coelute Baseline separation Adrin and fenthion coelute 10 20 10 20 5 15 9 14 12 17 9 14 fenthion (m/z 278) Aldrin and methylparathion (m/z 263) Ronnel (m/z 285) Heptachlor (m/z 272) Chlorpyrifos (m/z 314) Chlorphyifos methyl (m/z 286) Diazinon (m/z 137) Ronnel (m/z 285) Parathion (m/z 291) Fenthion (m/z 278) Separation of hexabromocylododecanes Selectivity for pesticides and thiophosphate esters ANALYSIS OF VOLATILES IN DRINKING WATER ON 25 M BP624 COLUMN 47 Components 6 1. Difluorobenzene 8 2. Benzene 3. Toluene - (d8) 4. Toluene 5. Chlorobenzene - (d5) 6. Ethyl benzene 7. p and m-Xylene 8. o-Xylene 9. Bromofluorobenzene 2 VOLATILES IN DRINKING WATER BP624, 1.2 µm 3 Phase: 25 m x 0.22 mm ID Column: 1 5 9 50 °C, 2 min 5 7 9 minutes Initial Temp: 15 °C/min Rate: 170 °C Final Temp: HP5970 MSD Detector: Hexadecane extract Injection Mode: He, 15 psi Carrier Gas: 054826 Column Part Number: Condition column at either 20 ° above the maximumEXPERT TIP method temperature or the recommended maximum column temperature (whichever is lower). 11 For further information on our full range of products, please visit www.sge.com

Environmental POLYCHLORINATED BIPHENYLS PCB ANALYSIS GC/MS Analysis of PCB mixture Phase: HT8, 0.25 µm Column: 25 m x 0.22 mm ID 3 Components Initial Temp: 60 °C, 2 min 2 1. PCB 18 Rate: 12 °C/min 2. PCB 31 Final Temp: 360 °C, 10 min 6 10 3. PCB 28 Detector: lon Trap MS 4. PCB 52 Injection Mode: PTV 9 5. PCB 44 Carrier Gas: He, 15 psi 6. PCB 70 14 7 11 7. PCB 101 8 8. PCB 110 9. PCB 149 12 10. PCB 153 11. PCB 138 5 12. PCB 180 13. PCB 170 13 Performance Specifications HT8 8% Phenyl (Equiv) Polycarborane - Siloxane 16:40 18:20 20:00 21:40 23:00 MINUTES Minimum Operating Temp.: -20 °C Maximum Cycling Temp.: 370 °C (Polyimide) Aroclor 1254 30 35 40 45 50 min Phase: HT8, 0.25 µm Column: 50 m x 0.22 mm ID Initial Temp: 60 °C Rate 1: 40 °C/min Temp 1: 200 °C Rate 2: 3 °C/min Temp 2: 320 °C Detector: ECD Carrier Gas: He, 40 psi 27 38 PCB ANALYSIS HT8, 0.25 µm film 26 Phase: 50 m x 0.22 mm ID Column: 90 °C, 1 min 30 34 56 Initial Temp: 20 °C/min 11 Rate 1: 170 °C,7.5 min Temp 1: 3.5 °C/min 36 58 Rate 2: 285 °C 37 Temp 2: 20 °C/min 42 Rate 3: 320 °C Temp 3: H₂, 43 cm/sec 9 63 Carrier Gas: ECD 31 Detector: 53 54 Components 1. PCB1 13 19 40 43 65 2. PCB2 18 44 64 3. PCB3 4. PCB10 25 5. PCB4 48 59 6. PCB9 7. PCB7 15 24 39 67 8. PCB6 60 9. PCB 8/5 8 45 51 10. PCB19 1 5 67 12 17 23 32 47 50 11. PCB18 41. PCB35 80. PCB144 119. PCB157 23 4 10 16 28 12. PCB11 42. PCB64 81. PCB147 120. PCB180 21 13. PCB17 43. PCB71/103 82. PCB135 121. PCB193 14 22 14. PCB13 44. PCB41 83. PCB77/82 122. PCB191 15. PCB24 45. PCB37 84. PCB149 123. PCB198 20 29 33 35 41 46 49 52 57 62 66 16. PCB27 46. PCB68 85. PCB124 124. PCB199 55 61 17. PCB15 47. PCB100 86. PCB143 125. PCB170 18. PCB32 48. PCB40 87. PCB134/ 126. PCB190/ 5 MINUTE S 20 19. PCB16 49. PCB57 107/131 196/203 20. PCB54 50. PCB67 88. PCB123 127. PCB169 84 95 120 21. PCB23 51. PCB63 89. PCB133 128. PCB208 103 125 22. PCB34 52. PCB102 90. PCB118 129. PCB207 111 23. PCB29 53. PCB95 91. PCB165 130. PCB195 106 24. PCB26 54. PCB74 92. PCB143/114 131. PCB189 25. PCB25 55. PCB121/ 93. PCB132/179 132. PCB194 93 26. PCB31 155/91 94. PCB122 133. PCB205 27. PCB8/53 56. PCB70 95. PCB153 134. PCB206 78 28. PCB51 57. PCB80 96. PCB176 135. PCB209 29. PCB21 58. PCB66 97. PCB141 Part Number: 79 97 102 112 30. PCB33/45/20 59. PCB96/55 98. PCB105 054676 31. PCB22 60. PCB84/92 99. PCB137 107 124 126 132 32. PCB46 61. PCB125 100. PCB130 33. PCB36 62. PCB90 101. PCB178 90 114 118 134 34. PCB52/69 63. PCB101 102. PCB163 75 83 133 135 35. PCB43 64. PCB60 103. PCB138 36. PCB49 65. PCB56 104. PCB160 70 92 98 130 40 37. PCB47/48/75 66. PCB152 105. PCB158/175 72 77 100 38. PCB44 67. PCB99 106. PCB187/182 80 109 117 121 39. PCB59 68. PCB119 107. PCB183/129 82 108 116 122 40. PCB42 69. PCB83 108. PCB126 70. PCB136 109. PCB185 87 91 128 129 131 71. PCB86 110. PCB159/202 68 72. PCB97 111. PCB174/128 73. PCB89 112. PCB177/201 85 74. PCB115 113. PCB167 75. PCB87 114. PCB171 69 71 73 74 76 81 86 88 89 94 99 104 105 110 113 115 119 123 127 76. PCB154 115. PCB197 77. PCB85 116. PCB173 78. PCB10/81 117. PCB200 79. PCB151 118. PCB156/172 25 MINUTE S For further information on our full range of products, please visit www.sge.com 12

Food, Flavors and Fragrances GC analysis in the Food, Flavors and Fragrances area covers a diverse range of compounds that vary in both polarity and boiling point. As a consequence a range of different columns are often required. Chromatograms are often complex, and any single column may not give enough separation of all of the compounds that may be present. Pairs of columns such as BPX5 and SolGel-WAX™ may be used to overcome this problem. For specific classes of compounds such as fatty acids, specialized columns are often necessary. Short chain fatty acids may be analyzed as free acids on the Nitroterephthalic acid (TPA) modified Polyethylene Glycol BP21 phase. Longer chain fatty acids are usually analyzed as fatty acid methyl esters on wax phases such as BP20 and SolGel-WAX™, or for more demanding applications, BPX70 or BPX90. Applications • Comparison of Geranium Oils on SolGel-WAX™ • Analysis of Eucalyptus Oil on SolGel-WAX™ • Analysis of Lavender Oil on Cydex-B • Analysis of Lavender Oil on BPX-5 • Analysis of Tasmanian Lavender Oil on SolGel-WAX™ • Analysis of Wine on BP20 • Analysis of Scotch Whisky on BP20 • Analysis of Teatree Oil on BPX5 • Analysis of Omega-3 Fatty Acids using BPX70 • BPX90 – a Highly Polar Phase for FAME Analysis • Analysis of PUFA-1 Marine FAME on BPX70 • Analysis of PUFA-2 Animal FAME on BPX70 • Analysis of PUFA-1 Marine FAME on BPX70 13 For further information on our full range of products, please visit www.sge.com

FoFoodo,d,FlFalvaovrosrsanadndFrFargargarnacnecses COMPARISON OF GERANIUM OILS ON SOLGEL-WAX™ EXPERIMENTAL CONDITIONS Column: SGE forte SolGel-WAX™, 30 m x 0.25 mm x 0.25 µm 5.00 10.00 15.00 20.00 25.00 30.00 (SGE P/N 054796) Comparison of total ion chromatograms (TIC) for Temperature Geranium oils from Reunion Island (green trace) and South Africa (red trace). Program: 60 °C for 3 min, then 4 °C/min to 220 °C Carrier Gas: He Flow: 0.6 mL/min Injection 0.3 µL split ratio 30:1 Volume Injection temp: 250 °C Liner: SGE P/N 092019 single tapered with quartz wool 5.00 10.00 15.00 20.00 25.00 30.00 ACKNOWLEDGEMENT Subtraction of the two TIC chromatograms shows the We thank M. Bernet and M. Didtsch of the ISIPCA Group, Research and difference between the two essential oils. Studies Centre for Fragrance, Cosmetics and Food Flavors, France, for providing these chromatograms. For more information see SGE application note AN-0020-C. ANALYSIS OF EUCALYPTUS OIL ON SOLGEL-WAX™ Components Phase: SolGel-WAX™, 0.25 µm film Sample: Neat 1. α-Pinene Column: 30 m x 0.25 mm ID 2. ß -Pinene Initial Temp: 40 °C, 1 min 3. Sabinene Rate 1: 8 °C/min to 220 °C 4. Limonene Final Temp: 220 °C, 5 min 5. 1,8 -Cineole Detector Type: Mass Spectrometer 6. p-Cymene Carrier Gas: He, 25.7 psi 7. Linalool L Carrier Gas Flow: 1.8 mL/min 8. Terpinen-4-ol Constant Flow: On Average Linear Velocity: 35 cm/sec at 40 °C 9. α-Terpineol Injection Mode: Split 10. α-Terpinenyl acetate Split Ratio: 100:1 Injection Volume: 0.2 µL 11. d-Carvoαne Injection Temperature: 250 °C Autosampler: No 5 Liner Type: 4 mm ID Single Taper Liner Liner Part Number: 092017 8000000 9 Column Part Number: 054796 7000000 6000000 1 ms-NoVent™ Part Number: 113400 5000000 HP5973 restrictor: 113409 4000000 4 8 10 Full Scan/SIM: 45-450 m/z 3000000 6 7 11 2000000 1000000 2 3 0 4.00 6.00 8.00 10.00 12.00 14.00 For further information on our full range of products, please visit www.sge.com 14

Food, Flavors and Fragrances ANALYSIS OF LAVENDER OIL ON CYDEX-B EXPERT TIP Better resolution is often obtained from chiral columns by using lower temperatures and higher carrier gas velocities than for conventional columns. Components 1. (+) Linalool 2. ( - ) Linalool LAVENDER OIL CYDEX-B, 0.25 µm film 50 m x 0.22 mm ID Phase: Column: Isothermal at 90 °C Initial Temp: FID Final Temp: 32 x 10-¹²AFS Detector: Split Injection Mode: 054901 Column Part Number: ANALYSIS OF LAVENDER OIL ON BPX5 Components 12. Camphor Phase: BPX5, 0.25 µm film 1. ß- Pinene 13. Lavandulol Lavender Oil: Lavender Oil in ethanol 2. 1-Octen-3-ol 14. Borneol L Column: 30 m x 0.25 mm ID 3. 3-Octanol 15. Terpinen-4-ol 40 °C, 1 min 4. 3-Octanol Initial Temp: 5 °C/min to 260 °C 5. p-Cymene 16. α-Terpineol Rate 1: 260 °C 6. Limonene Final Temp: Mass Spectrometer 7. cis-Ocimene 17. Linalyl acetate Detector Type: He, 7.0 psi 8. cis-Linaloxide 18. Lavundyl Acetate Carrier Gas: 1.0 mL/min 9. trans-Linalool oxide 19. Geranyl Acetate Carrier Gas Flow: On 10. Linalool L Constant Flow: 36 cm/sec at 40 °C 11. Octenyl acetate 20. α-Santalene Average Linear Velocity: Split Injection Mode: 200:1 21. ß-Caryophyllene Split Ratio: 200 mL/min Purge on (Split) Vent Flow: 0.2 µL 22. α-Cadinine Injection Volume: 250 °C 23. α-Cryophyllene oxide Injection Temperature: No Autosampler: 4 mm ID Double Taper Liner 280000 10 17 Liner Type: 092018 240000 18 Liner Part Number: 054101 Column Part Number: 200000 3 15 21 SolGel-WAX™, 0.25 µm film 160000 11 Neat 30 m x 0.25 mm ID 120000 40 °C, 1 min 8 °C/min to 220 °C 80000 16 14 20 23 220 °C, 5 min 40000 16 19 Mass Spectrometer 24 89 He, 25.7 psi 0 7 13 22.0 1.8 mL/min 22 On 5 12 35 cm/sec at 40 °C 16.0 Split 10.0 12.0 14.0 18.0 20.0 24.0 26.0 28.0 100:1 0.2 µL ANALYSIS OF TASMANIAN LAVENDER OIL SOLGEL-WAX™ 250 °C No 1.4e+07 Components Phase: 4 mm ID Single Taper Liner 1.2e+07 1. 3-Octanone Sample: 092017 2. Octenyl acetate Column: 054796 1e+07 3. Octanol 113400 8000000 4. cis Linalool oxide Initial Temp: 113409 6000000 5. trans Linalool oxide Rate 1: 45-450 m/z 4000000 6. Linalool L Final Temp: 2000000 7. Linalyl acetate Detector Type: 8. Terpinen-4-ol Carrier Gas: 0 9. Lavandulyl acetate Carrier Gas Flow: 10. Borneol L Constant Flow: 11. Caryophyllene oxide Average Linear Velocity: Injection Mode: 6 Split Ratio: 7 Injection Volume: Injection Temperature: 18 Autosampler: 29 Liner Type: 45 10 11 Liner Part Number: 3 Column Part Number: 4.00 6.00 8.00 10.00 12.00 14.00 16.00 ms-NoVent™ Part Number: HP5973 restrictor: Full Scan/SIM: 15 For further information on our full range of products, please visit www.sge.com

FoFoodo,d,FlFalvaovrosrsanadndFrFargargarnacnecses ANALYSIS OF WINE ON BP20 58 9 Components 1. Acetaldehyde 3 7 2. Ethyl Acetate Phase: BP20, 1.0 µm 2 6 3. Methanol Column: 25 m x 0.32 mm ID 14 4. Isopropanol 5. Ethanol Initial Temp: 40 °C, 2 min 6. Propanol Rate 1: 5 °C/min 7. Isobutanol Temp 2: 50 °C 8. Isoamyl Alcohol Rate 2: 15 °C/min 9. Acetic Acid Final Temp: 190 °C 10. Unknown Carrier Gas: H₂,6psi Injection Mode: 2 µL 10 Column Part Number: 054442 0 5 10 minutes ANALYSIS OF SCOTCH WHISKY ON BP20 EXPERT TIP For extended life of polar columns, always use an oxygen trap in the carrier gas line. 47 6 5 12 Components Phase: BP20, 1.0 µm 3 1. Acetaldehyde Column: 12 m x 0.53 mm ID 2. Ethyl Acetate 0 3 6 minutes 3. Methanol Initial Temp: 55 °C, 3 min 4. Ethanol Rate: 10 °C/min 5. Propan-1-ol Final Temp: 120 °C, 0 min 6. 2-Methylpropan-1-ol Detector: FID 7. 2-Methylbutan-1-ol+3- Sensitivity: 128 x 10-¹²AFS Methylbutan-1-ol Injection Mode: Split Column Part Number: 054447 ANALYSIS OF TEATREE OIL ON BPX5 Components 13. Terpinen-4-ol Phase: BP20, 0.25 µm film 1. Thujene Column: 30 m x 0.25 mm ID 14. α-Terpineol 2. α-Pinene 15. α -Gurjunene Initial Temp: 40 °C, 1 min Rate 1: 5 °C/min to 200 °C 3. Sabinene 16. (trans)-ß-Caryophyllene Final Temp: 200 °C 4. 3-Octanol 17. Aromadendrene Detector Type: Mass Spectrometer 5. ß-Pinene 18. Alloaromadendrene Carrier Gas: He, 7.0 psi 19. Ledene Constant Flow: 1.0 mL/min. 6. α-Phellandrene 20. Germacrene B Average Linear Velocity: 36 cm/sec at 40 °C 7. α-Terpinene Injection Mode: Split 21. α-Cadinene Split Ratio: 200:1 8. p-Cymene Purge on (Split) Vent Flow: 200 mL/min. 9. Limonene 22. 1s, cis-Calamenene Injection Volume: 0.2 µL 10. 1,8-Cineole 23. Globulol Injection Temperature: 250 °C 11. y-Terpinene Autosampler: No 1.2e+07 12. Terpinolene Liner Type: 4 mm ID Double Taper Liner Liner Part Number: 092018 13 Column Part Number: 054101 11 1e+07 7 8000000 10 14 19 8 18.0 20 6000000 12 4000000 2 2000000 9 0 1 15 17 21 8.0 45 18 22 23 36 16 10.0 12.0 14.0 16.0 20.0 22.0 24.0 26.0 28.0 For further information on our full range of products, please visit www.sge.com 16

Food, Flavors and Fragrances ANALYSIS OF OMEGA-3 FATTY ACIDS USING A BPX70 Components 18. C18:2 trans 9,12 Phase: BPX70, 0.25 µm film 19. C18:2 cis 9,12 Sample: 10 ppm in methanol 1. C6 20. C18:3 cis 6,9,12 Column: 25 m x 0.32 mm ID 2. C8 21. C18:3 cis 9,12,15 80 °C, 2 min 3. C10 22. C18:4 cis 6,9,12,15 Initial Temp: 50 °C/min to 130 °C, 10 min 4. C11 23. C20 Rate 1: 2 °C/min to 172°C 5. C12 24. C21:1 cis 11 Rate 2: 172 °C , 6 min 6. C13 25. C20:2 cis 11,14 Final Temp: FID 7. C14 26. C20:3 cis 8,11,14 Detector Type: 300 °C 8. C14:1 cis 9 27. C20:4 cis 5,8,11,14 Detector Temp: He, 10 psi 9. C15 28. C20:3 cis 11,14,17 Carrier Gas: 2.2 mL/min. 10. C16 29. C22 Carrier Gas Flow: On 3 11. C16:1 cis 9 30. C20:5,8,11,14,17 Constant Flow: 39 cm/sec at 80 °C 31. C22:1 cis 13 Average Linear Velocity: Split 12. C17 32. C22:4 cis 7,10,13,16 Injection Mode: 58:1 13. C17:1 cis 10 33. C24 Split Ratio: 1 µL 14. C18 34. C22:6 cis Injection Volume: 250 °C 15. C18:1 trans 9 4,7,10,13,16,19 Injection Temperature: No 16. C18:1 cis 9 Autosampler: 4 mm ID Focus Liner™ 17. C18:1 cis 12 Liner Type: 092002 Liner Part Number: 054606 pA 5 Column Part Number: ACKNOWLEDGEMENT 45 2 The chromatogram shows the excellent separation SGE would like to thank Masterfoods 40 1 6 of a complex mixture of FAME compounds. UK for supplying the sample and 35 chromatographic conditions for this Note the excellent peak shape and separation chromatogram. For more information of the Omega-1,2 and 3 fatty acid isomers both see SGE technical poster TP-0100-C 7 structural and cis and trans. 30 8 9 25 10 15 20 11 13 14 16 18 19 20 26 27 29 31 23 24 28 33 15 21 25 32 17 12 30 34 10 22 5 55 10 15 20 25 30 35min BPX90 – A HIGHLY POLAR PHASE FOR FAME ANALYSIS trans C23:0 C22:1n9 C20:5n3 (EPA) C22n6 (DHA) cis C22:2 BPX70 C18:0 CC1188::22nn66 C18:3n6 C20:2 C24:0 C24:1 C18:1n9 trans C18:3n3 C20:3n6 C20:4n6 C20:3n3 cis C14:1 C17:0 C17:1 C18:n9 C20:0 C20:1 FAME POLARITY TEST C15:0 C15:1 C14:0 C16:0 C16:1 C21:0 C22:0 trans C20:2 C22n6 (DHA) WHAT IS DIFFERENT ? cis C20:3n6 • BPX90 is a highly polar phase of the C20:3n3 poly (biscyanopropylsiloxane) type. CC1188::22nn66 C18:3n6 C18:3n3 C20:4n6 • The phase has excellent thermal C22:2 stability and a wide operating range C20:5n3 (EPA) (70 - 280 °C). BPX90 C18:1n9 trans • The separation mechanisms gives cis C22:1n9 short elution times relative to other Supelco 37 FAME test mixture. Columns 15 m x 0.25 mm C24:1 polar phases. BPX90 shows low ID x 0.25 micron film. Temperature programmed 70°CC15:0 C14:1C16:0 C15:1C16:1C18:0 C17:1C18:n9C21:0 C20:1 selectivity for non-polar analytes and (hold 1 min) to 150 °C (20 °C/min) to 250 °C (10 °C/min)C17:0 saturated FAME. C14:0then hold at 250 °C (5 min). Injector: 240 °C. C20:0 C22:0 • BPX90 shows enhanced selectivity for Detection MS. C23:0 polyunsaturated FAME and the C24:0 selectivity can be tuned with film thickness. • BPX90 is effective for the separation of cis and trans isomers and positional isomers of FAME analytes. C18-C22 FAME test mixture. Columns 30 m x 0.25 mm ID x 0.25 micron film. Isothermal: 180°C. Injector: 240 °C. Detection FID at 280 °C. 17 For further information on our full range of products, please visit www.sge.com

FoFoodo,d,FlFalvaovrosrsanadndFrFargargarnacnecses ANALYSIS OF PUFA-1 MARINE FAME ON BPX70 13 13 Components EXPERT TIP 2 4 1. C14:0 To fully utilize the high thermal stability of BPX70 5 2. C16:0 columns SGE recommend the use of helium when 3. C16:1n7 operating above 220/230°C for extended periods. 6 10 12 4. C18:1n9 9 5. C18:1n7 6. C 18:2n6 8 7. C18:3n3 7 11 8. C18:4n3 9. C20:0 14 16 10. C20:1n9 11. C20:4n6 Phase: BPX70, 0.25 µm film 12. C20:5n3 Column: 25 m x 0.22 mm ID 15 Initial Temp: 150 °C, 0 min Program Rate: 2 °C/min Final Temp: 210 °C, 5 min Carrier Gas: He, 15 psi Detector: FID, 280 °C Sensitivity: 32 x 10-¹²AFS Injection Mode: Split, 50:1 Column Part Number: 054602 0 15 30 minutes ANALYSIS OF PUFA-2 ANIMAL FAME ON BPX70 2 57 12 Components 9. C20:0 4 1. C14:0 10. C20:1n11 2. C16:0 11. C20:3n6 3. C16:1n7 12. C20:4n6 4. C18:0 13. C20:5n3 5. C18:1n9 14. C22:4n6 6. C 18:2n7 15. C22:5n3 7. C18:2n6 16. C22:6n3 8. C18:3n3 6 9 13 16 PUFA-2 ANIMAL FAME BPX70, 0.25 µm film 8 10 11 15 Phase: 25 m x 0.22 mm ID 14 Column: 150 °C, 0 min 13 Initial Temp: 2 °C/min Program Rate: 210 °C, 5 min Final Temp: He, 15 psi Carrier Gas: FID, 280 °C Detector: 32 x 10-¹²AFS Sensitivity: Split, 50:1 Injection Mode: 054602 Column Part Number: 0 15 30 minutes ANALYSIS OF PUFA-1 MARINE FAME ON BPX70 1 10 Components 6. C 20:0 1. C18:1n9 7. C20:1n9 2. C18:1n7 8. C20:4n6 3. C18:2n6 9. C20:5n3 4. C18:3n3 10. C22:1n11 5. C18:4n3 2 6 9 PUFA-2 ANIMAL FAME BPX70, 0.25 µm 3 57 8 Phase: 50 m x 0.22 mm ID Column: 4 30 150 °C, 0 min Initial Temp: 2 °C/min Program Rate: 210 °C, 5 min Final Temp: He, 15 psi Carrier Gas: FID, 280 °C Detector: 32 x 10-¹²AFS Sensitivity: Split, 50:1 Injection Mode: 054603 Column Part Number: 15 minutes For further information on our full range of products, please visit www.sge.com 18

Fuels and Petrochemical For Fuels and Petrochemical analysis by GC, one of the main considerations is the thermal stability of the column, both physical and chemical. Phases must have high temperature limits to allow the analysis of high boiling point compounds and columns must be able to physically withstand repeated cycling to extreme temperatures. Columns such as SGE’s BPX1 and HT5 have been created with these demands in mind. Where higher polarity is required, such as the separation of aromatic compounds, SolGel-WAX™ and BPX90 provide enhanced selectivity without the unnecessary sacrifice of maximum temperature limits. Applications • Total Recoverable Petroleum Hydrocarbons (TRPH) Analysis on Standard and Fast BPX5 • Analysis of Polywax 655 and Refinery Lubrication Oil on HT5 • The Separation of Aromatics from Olefins in Petroleum Samples using BPX90 • Unleaded Gasoline on BPX5 • Fast GC For TPH Analysis • Simulated Distillation using BPX1-SimD 19 For further information on our full range of products, please visit www.sge.com

Fuels and Petrochemical TOTAL RECOVERABLE PETROLEUM HYDROCARBONS (TRPH) ANALYSIS ON STANDARD AND FAST BPX5 NORMAL FAST Chromatogram showing separation of Total Recoverable Chromatogram showing separation of Total Recoverable Petroleum Hydrocarbons using a conventional 30 m x 0.25 mm Petroleum Hydrocarbon using a FAST BPX5 column. ID BPX5 column with a 0.25 micron film. C8 C8 C10 C10 C12 C12 C14 C14 C16 C16 C18 C18 C20 C20 C22 C22 C24 C24 C26 C30 C28C26 C28 C30 C32 C32 C34 C34 C36 C36 C38 C38 C40 C40 0 minutes 20 0 minutes 10 Phase: BPX5, 0.25 µm film Phase: BPX5, 0.10 µm film TRPH (C8-C40): 5 ng/µL in dichloromethane TRPH (C8-C40) Standard: 5 ng/µL in dichlormethane Column: 30 m x 0.25 mm ID Column: 10 m x 0.10 mm ID Initial Temp: 40 °C , 2 min Initial Temp: 40 °C , 1 min Rate 1: 30 °C/min to 330 °C Rate 1: 30 °C/min to 330 °C Rate 2: NA Rate 2: NA Final Temp: 330 °C, 9 min Final Temp: 330 °C, 0 min Detector Type: FID, 350 °C Detector Type: FID, 350 °C Carrier Gas: He, 14.4 psi Carrier Gas: He, 28 psi Carrier Gas Flow : 1.29 mL/min Carrier Gas Flow : 0.52 mL/min Constant Flow: On Constant Flow: On Average Linear Velocity: 40 cm/sec at 40 °C Average Linear Velocity: 55 cm/sec at 40 °C Injection Mode: Split, 120:1 Injection Mode: Split, 120:1 Purge On Time: NA Purge On Time: NA Purge On (Split) Vent Flow: 160 mL/min Purge On (Split) Vent Flow: 62 mL/min Injection Volume: 1µL Injection Volume: 1 µL Injection Temperature: 250 °C Injection Temperature: 250 °C Autosampler: Yes Autosampler: Yes Liner Type : 4 mm ID FocusLiner™ Liner Type : 2.3 mm ID FocusLiner™ with single taper Liner Part Number: 092003 Liner Part Number: 092005 Column Part Number: 054101 Column Part Number: 054099 ANALYSIS OF POLYWAX 655 AND REFINERY LUBRICATION OIL ON HT5 POLYWAX 655 AND REFINERY LUBRICATION OIL Phase: HT5, 0.1 µm Column: 6 m x 0.53 mm ID Initial Temp: 50 °C Rate: 10 °C/min Final Temp: 480 °C, 15 min Detector: FID Sensitivity: 40 x 10-¹² AFS Injection Mode: On-Column POLYWAX 655 Carrier Gas: H2, 20 mL/min Solvent: CS₂ Column Part Number: 054661 REFINERY LUBRICATION OIL 50 TEMPERATURE(°C) 480 320 450 570 650 700 750 boiling point (0°C) For further information on our full range of products, please visit www.sge.com 20

Fuels and Petrochemical THE SEPARATION OF AROMATICS FROM OLEFINS IN PETROLEUM SAMPLES USING BPX90 The separation of a petroleum sample using a BPX90 column (30 m x 0.25 mm ID, 250 micron film) showing the resolution of aromatic families and the separation from more abundant alkanes. UNLEADED GASOLINE ON BPX5 Phase: BPX5, 0.25 µm film Column: 30 m x 0.25 mm ID Column Part Number: 054101 Components Sample Introduction: 240 °C 1. Benzene Injector Temp: 0.1 µL 2. Toluene Injection Volume: 0.5 µL Removable Needle 3. Ethylbenzene Autosampler Syringe: Part No. 000410 4. m,p-Xylene 5. o-Xylene 2 Septa: Auto-Sep T™ Part No. 041882 Injection Type: Split Purge On Time: NA Purge On (Split) Vent: 200 mL/min Split Ratio: 149 :1 Liner Type: FocusLiner™ single taper Part No.092003 He Pressure/Flow Values: On Carrier Gas: 13.6 psi Constant Flow: 1.34 mL/min Pressure: 30 cm/sec @ 25 °C Column Flow: Linear Velocity: 4 Oven Parameters: 25 °C 1 Initial Temp: 1 min Initial Time: 30 °C/min 5 Rate 1: 240 °C 3 Final Temp 1: 1 min Hold Time: 9.17 min Run Time: Detector Parameters: FID @ 280 °C Detector Type: 0 2 4 6 min 21 For further information on our full range of products, please visit www.sge.com

Fuels and Petrochemical FAST GC FOR TPH ANALYSIS Phase: BPX5, 0.1 µm pA Benzene Column: 5 m x 0.1 mm ID 140 p-m, xylene Initial Temp: 50 °C Rate 1: 45 °C/min 100 Toluene Final Temp: 300 °C, 0 min pA Detector Temp: 270 °C 60 Ethyl Benzene Detector: FID C8 o- xylene 140 Carrier: He, Inlet pressure 40 psi (constant flow mode, linear velocity of 75 cm/sec) C8 10 12 13 14 16 9 15 100 11 17 19 30 C32 18 21 23 26 28 20 25 22 24 60 C12 20 20 01 2 3 4 5 6 minutes 0 0.5 1 1.5 2 minutes Chromatogram of TPH standards from C8 to C32 Chromatogram showing elution of BTEX in under one minute SIMULATED DISTILLATION USING BPX1-SIMD Phase: BPX1, 0.1 µm Column: 5 m x 0.53 mm ID C20 C40 Initial Temp: 40 °C Rate: 15 °C C60 Final Temp: 420 °C, 5 min Detector Temp: 440 °C C80 Carrier Gas: He, 10 mL/min C100 Instrument: HP 6890 Column Part Number: 054800 0 10 20 minutes Separation Systems Injector 40 °C Standard mix for HTSD using BPX1-SimD Initial Temp: 15 °C Rate: 420 °C, 5 min C40 C60 Final Temp: A portion of the previous chromatogram from C40 to the end of the analysis (expanded vertically) shows excellent resolution and the ability to see beyond C110. C80 C100 All of the data presented was produced by Dr. Lubkowitz and the staff C90 C110 at Separation Systems Inc. on a system using the Separation System programmed temperature vaporization injector (PTV) and the SIMDIS 0 10 20 minutes EXPERT® software Enlarged section of the chromatogram above Gas Oil Reference 420˚C pA IBP 0.6 400 5% -6.2 10pA base line 10% -3.4 200 20% -2.9 30% -1.1 40% 0.7 50% 1.1 60% 2.1 70% 2.3 80% 1.8 90% 1.2 95% 0.2 FRP 0.3 25pA 3.2 min 21.277 min 30 0 10 minutes 20 0 10 minutes 20 30 CS2 Blank Reference Gas Oil MT-60 For further information on our full range of products, please visit www.sge.com 22

Chemical For general chemical analysis, a good rule of thumb is to use the lowest polarity column that provides sufficient separation. Particular classes of compounds, such as alcohols, amines, or organic acids, may require thicker film phases, or specific phases such as the BPX35 or BP21 to avoid undue peak tailing. Applications • Analysis of 18 Alcohols on BP20 • Analysis of Aliphatic Alcohols on BP1 • Analysis of 15 Organic Acids on BP20 • US EPA 625 Phenols Mix on BPX50 • Analysis of Organic Acids in Water on BP21 • Analysis of Amines on BP1 • Analysis of Aromatic Amines on BP5 • Analysis of Aromatic Amines from Diazo Dyes on BPX35 • Analysis of Ketones on Thick Film BPX5 • Analysis of Triethylamine and Triethanolamine on SolGel-1ms™ 23 For further information on our full range of products, please visit www.sge.com

Chemical ANALYSIS OF 18 ALCOHOLS ON BP20 5 Components EXPERT TIP 1. Methanol 12 6 12 2. Propan-2-ol After installing a new column purge with oxygen free 4 8 11 3. Ethanol carrier gas for at least 30 minutes before heating GC oven. 9 4. sec-Butan-1-ol 3 15 16 17 5. n-Propanol + 7 10 14 2-Methyl-3-Buten-2-ol 18 6. d,l-3-Methyl-2-Butan-1-ol ALCOHOLS 7. Pentan-3-ol Phase: 13 8. d,l-2-Pentan-1-ol Column: BP20, 0.25 µm film 9. n-Butanol 30 m x 0.25 mm ID 10. 2,4-Dimethyl Initial Temp: Pentan-3-ol Rate: 45 °C, 2 min 11. Hexan-3-ol Final Temp: 3 °C/min 12. 2-Methyl Prop- Detector: 80 °C, 0 min 2-en-1-ol Sensitivity: FID 13. Crotyl Alcohol Injection Mode: 128 x 10-¹² AFS (2-Buten-1-ol) Column Part Number: Split 14. Hexan-2-ol 054427 15. Pentan-1-ol 16. 2-Methyl Pentan-1-ol 17. 2-Ethyl Butan-1-ol 18.Hexan-1-ol 0 2 4 6 8 10 12 minutes ANALYSIS OF ALIPHATIC ALCOHOLS ON BP1 1 23 7 ALCOHOLS BP1, 3.0 µm film 5 Phase: 12 m x 0.53 mm ID 6 Components Column: 1. Octanol 100 °C 4 2. Decanol Initial Temp: 10 °C/min 3. Undecanol Rate: 260 °C 4. Dodecanol Final Temp: N2 5. Tetradecanol Carrier Gas: 0.1 µL 6. Hexadecanol Injection Volume: 054097 7. Eicosanol Column Part Number: 0 minutes 10 ANALYSIS OF 15 ORGANIC ACIDS ON BP20 Components 9. Tridecanoic Acid 1. Valeric Acid 10. Tetradecanoic Acid 2. Caproic Acid 11. Pentadecanoic Acid 3. Heptanoic Acid 12. Hexadecanoic Acid 4. Octanoic Acid 13. Heptadecanoic Acid 5. Nonanoic Acid 14. Octadecanoic Acid 6. Decanoic Acid 15. Arachidic Acid 7. Undecanoic Acid 8. Dodecanoic Acid 1 9 ORGANIC ACIDS BP20, 0.25 µm 2 78 Phase: 30 m x 0.32 mm ID Column: 70 °C 3 10 10 °C/min 5 11 Initial Temp: 260 °C, 5 min Rate: FID 4 Final Temp: Split Detector: H2, 6 psi 6 12 Injection Mode: 054433 Carrier Gas: 13 Column Part Number: 14 15 0 minutes 10 20 For further information on our full range of products, please visit www.sge.com 24

Chemical US EPA 625 PHENOLS MIX ON BPX50 5 Components 6 1. 2-Chlorophenol 2. 2-Nitrophenol 24 3. 2, 4-Dimethylphenol 4. 2, 4-Dichlorophenol 3 5. 4-Chloro-3-methylphenol 6. 2, 4, 6-Trichlorophenol 7. 2, 4- Dinitrophenol 8. 4-Nitrophenol 9. 2-Methyl-4, 6-dinitrophenol 10. Pentachlorophenol 10 89 7 Abundance1 US EPA 625 PHENOLS MIX Phase: 30000 EXPERT TIP Column: BPX50, 0.25 µm 20000 30 m x 0.25 mm ID 10000 Injector Mode: Initial Oven Temp: Split, 40:1 Rate 1: 50°C, 1 min Final Temp: 8 °C/min Detector: 300 °C, 10 min Column Part Number: HP 5973 MSD 054751 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 ANALYSIS OF ORGANIC ACIDS IN WATER ON BP21 9 On-column injection and the addition of a 0.03M Oxalic acid (2%) to the injection solution increases the acidity Components of the column to allow lactic acid to be detected. 1. Acetic Acid 8 2. Propanoic Acid 3. iso-Butyric Acid 5 6 7 4. n-Butyric Acid ORGANIC ACIDS IN WATER 34 5. iso-Valeric Acid (0.03M OXALIC ACID) 6. n-Valeric Acid Phase: BP21, 0.5 µm film 2 7. n-Caproic Acid Column: 30 m x 0.53 mm ID 8. n-Heptanoic Acid 9. Lactic Acid Initial Temp: 85 °C, 0 min Rate: 6 °C/min 1 Final Temp: 180 °C, 5 min Detector: FID 0 5 10 15 MINUTES Sensitivity: 64 x 10-¹² AFS Injection Mode: On-Column Column Part Number: 054477 ANALYSIS OF AMINES ON BP1 1 2 Components ANALYSIS OF AMINES BP1, 3.0 µm film 1. Aniline Phase: 12 m x 0.53 mm ID 3 2. Decylamine Column: 4 3. Dicyclohexylamine 70 °C 5 4. Dodecylamine Initial Temp: 10 °C/min 5. Tetradecylamine Rate: 250 °C Final Temp: N2 Carrier Gas: 0.1 µL Injection Volume: 054097 Column Part Number: 0 minutes 10 25 For further information on our full range of products, please visit www.sge.com

ANALYSIS OF AROMATIC AMINES ON BP5 Chemical Components 7. 2,6-Dimethylaniline EXPERT TIP Using a thicker film helps prevent amines from tailing. 1. Pyridine 8. 1,4-Phenyldiamine 2. 2-Methyl Pyridine 9. Nicotine 3. gamma - BHC 10. Biphenylamine 4. Aniline 11. Bibenzylamine 5. o-Toluidine 6. m-Toluidine 89 10 11 3 6 7 Phase: BP5, 1.0 µm film 45 Column: 12 m x 0.53 mm ID Initial Temp: 60 °C, 0 min Rate: 10 °C/min Final Temp: 190 °C, 0 min Detector: FID Sensitivity: 128 x 10-¹²AFS Injection Mode: Split Column Part Number: 054197 0 6 MINUTES 12 ANALYSIS OF AROMATIC AMINES FROM DIAZO DYES ON BPX35 Components 13. 4,4’-Diaminodiphenylmethane Phase: BPX35 0.25 µm film 1. Indolin 14. Benzidine Azo Dyes Standard: 10 ppm solution in DCM 2. o-Toluidine 15. 3,3’-Dimethyl- Column: 30 m x 0.25 mm ID 3. 2,4-Diaminoanisole 4. p-Chloroailine 4,4’diaminodiphenyl- Initial Temp: 50 °C, 2 min 5. p-a residine methane Rate 1: 15 °C/min to 240 °C 6. 2,4,6-Trimethylaniline 16. 3,3’-dimethylbenzidine Rate 2: 10 °C/min to 280 °C 7. 4-Chlorotoludine 17. 4,4’-Thiodianiline Final Temp: 280 °C, 25 min 8. Unknown 18. 3,3’-Dichlorobenzidine Detector Type: MSD 9. 2-Naphtylamine 19. 4,4’-Methylene- Carrier Gas: He, 7.1 psi 10. Unknown bis(2chloraniline) Carrier Gas Flow : 1.0 mL/min 11. 4-Aminodiphenyl 20. 3,3’-Dimethoxybenzidine Constant Flow: On 12. 4,4’-Oxydianiline Average Linear Velocity: 36 cm/sec at 50 °C Injection Mode: Splitless 800000 7 11 Purge On Time: 1.0 min 700000 Purge On (Split) Vent Flow: 60 mL/min 600000 45 Injection Volume: 1 µL 500000 Injection Temperature: 250 °C 400000 6 Autosampler: No 300000 2 Liner Type : 4 mm ID Double Taper 200000 Liner Part Number: 092018 100000 9 18, 19, 20 Column Part Number: 054701 17 0 1 8 10 13 15 3 14 16 12 10.0 15.0 20.0 25.0 30.0 SilTite™ ferrules eliminate the need for re-tighteningEXPERT TIP following temperature cycling and reduce oxygen levels within the system improving performance 26 For further information on our full range of products, please visit www.sge.com

Chemical Chemical Analysis ANALYSIS OF KETONES ON THICK FILM BPX5 Components 13. 2-Methyl-3-hexanone Phase: BPX5, 1.0 µm film 1. Ethanol 14. 4-Methyl-2-hexanone Sample: 300 ppm in dichloromethane 2. Acetone 15. 5-Methyl-2-hexanone Column: 60 m x 0.25 mm ID 3. 2-Butanone 16. 3-Heptanone Initial Temp: 4. 3-Methyl-2-butanone 17. 2-Heptanone Rate 1: 40 °C , 5 min 5. 2-Pentanone 18. Cyclohexanone Rate 2: 10 °C/min to 80 °C 6. 3-pentanone 19. 2-Octanone Final Temp: 30 °C/min to 260 °C 7. 4-Methyl-2-pentanone 20. 2-Nonanone Detector Type: 260 °C, 4 min 8. 3-Methyl-2-pentanone 21. 2-Decanone Detector Temp: FID 9. 3-Hexanone 22. 2-Undecanone Carrier Gas: 360 °C 10. 2-Hexanone 23. 2-Dodecanone He, 27.6 psi 11. Mesityl oxide 1.9 mL/min 12. Cyclopentanone On 35 cm/sec at 40 °C Carrier Gas Flow : Split 100:1 pA 20 Constant Flow: 0.4 µL 60 250 °C 50 Average Linear Velocity: No 40 4 mm ID Single Taper Liner 30 16 17 18 21 Injection Mode: 092017 20 22 054123 10 19 Split Ratio: 14 4 Injection Volume: 1012 15 9 78 Injection Temperature: 5 6 13 Autosampler: 3 2 11 1 6 4 23 Liner Type : Liner Part Number: Column Part Number: 8 10 12 14 16 min ANALYSIS OF TRIETHYLAMINE AND TRIETHANOLAMINE ON SOLGEL-1ms™ Phase: SolGel-1ms, 0.25 µm film 10 ng/µL in dichloromethane Amine mix: 30 m x 0.32 mm ID Column: 40 °C , 5.0 min 20 °C/min to 200 °C Initial Temp: 200 °C, 7 min FID Rate 1: 300 °C He, 9.9 psi Final Temp: 2.2 mL/min On Detector Type: 35 cm/sec at 100 °C Split Triethylamine Detector Temp: 50:1 Carrier Gas: 0.3 µL Solvent Carrier Gas Flow : 250 °C No counts 054798 30000 Constant Flow: 25000 Average Linear Velocity: Triethanolamine Mode of Injection: 20000 Split Ratio: 15000 Injection Volume: 10000 Injection Temperature: Autosampler: 5000 Column Part Number: 0 2 4 6 8 10 12 14 16 18 min EXPERT TIP To prevent decreasing retention times in your chromatography, replace the septum daily. 27 For further information on our full range of products, please visit www.sge.com

Pharmaceutical GC analysis of Pharmaceuticals covers a wide range of compounds that can vary greatly in their molecular weight, reactivity, and pH. From the analysis of low molecular weight residual solvents on a G43 (BPX-Volatiles) to higher molecular weight compounds on a G42 (BPX35), a wide range of GC columns are often specified in the test methods. Proper deactivation of GC consumables such as liners and columns becomes increasingly important where system inertness has to be demonstrated. SGE’s unique, high temperature gas phase deactivation ensures maximum inertness and minimal activity from our columns and consumables. Applications 28 • USP Methods • Analysis of Tricyclic Antidepressants on BPX35 • Analysis of Dioxane Impurities on BP20 • Analysis of a Common Solvent Mixture on a Thick Film BPX5 • Analysis of a Common Pharmaceutical Solvent on BPX-Volatiles • Analysis of a Common Pharmaceutical Solvent on BPX-Volatiles • Analysis of Class I Solvents on BPX-Volatiles • Analysis of the Separation of the Class III Solvents on BPX-Volatiles For further information on our full range of products, please visit www.sge.com

USP Methods Method Phase Composition SGE Phase Recommendation G1 Dimethylpolysiloxane oil BP1, SOLGEL-1ms™ G2 Dimethylpolysiloxane gum BP1, SOLGEL-1ms G3 50% Phenyl - 50% Methylpolysiloxane BPX50 G5 3-Cyanopropylpolysiloxane BPX70 G7 50% 3-Cyanopropyl - 50% Phenylmethylsilicone BP225 G14 Polyethylene glycol (average molecular weight of 950-1,050) BP20(WAX), SOLGEL-WAX™ G15 Polyethylene glycol (average molecular weight of 3,000-3,700) BP20(WAX), SOLGEL-WAX G16 Polyethylene glycol (average molecular weight of 15,000) BP20(WAX), SOLGEL-WAX G17 75% Phenyl - 25% Methylpolysiloxane BPX50 G19 25% Phenyl - 25% Cyanopropylmethylsilicone BP225 G20 Polyethylene glycol (average molecular weight of 380-420) BP20(WAX), SOLGEL-WAX G25 Polyethylene glycol TPA (Carbowax 20M terephthalic acid) BP21(FFAP) G27 5% Phenyl - 95% Methylpolysiloxane BP5, BPX5 G28 25% Phenyl - 75% Methylpolysiloxane BPX35 G32 20% Phenylmethyl - 80% Dimethylpolysiloxane BPX35 G35 Polyethylene glycol & diepoxide esterified with nitroterephthalic acid BP21(FFAP) G36 1% Vinyl - 5% Phenylmethylpolysiloxane BP5, BPX5 G38 Phase G1 plus a tailing inhibitor BP1, SOLGEL-1ms G39 Polyethylene glycol (average molecular weight of 1,500) BP20(WAX), SOLGEL-WAX G41 Phenylmethyldimethylsilicone (10% phenyl substituted) BP5, BPX5 G42 35% Phenyl - 65% Dimethylvinylsiloxane BPX35 G43 6% Cyanopropylphenyl - 94% Dimethylpolysiloxane BP624 G46 14% Cyanopropylphenyl - 86% methylpolysiloxane BP10 (1701) Fused Silica Tubing ETP multiplier • Quality guaranteed • Air stable • Chemically inert and thermally stable • 2 year shelf life guarantee • Suitable for organic and aqueous solvents • Discrete dynode design results in extend operating life • Ideal for biotechnology applications • Total compatibility with all major quadrupole, • Custom-made tubing available upon request • Available deactivated for guard column material magnetic sector and TOF instruments • Tubing protected with a high temperature Polyimide resin (+400 ˚C) 29 For further information on our full range of products, please visit www.sge.com

Pharmaceutical Pharmaceutical ANALYSIS OF Tricyclic Antidepressants on BPX35 Components 1. Amitriptyline 2. Trimipramine 3. Nortriptyline 4. Doxepin 5. Desipramine TRICYCLIC ANTIDEPRESSANTS BPX35, 0.25 μm Phase: 25 m x 0.22 mm ID Column: 210 °C, 1 min Initial Temp: 5 °C/min Rate: 280 °C Final Temp: Helium, 150 kpa Carrier Gas: Split (20:1) Injection Mode: FID, 380 °C Detector: 054711 Column Part Number: analysis of Dioxane Impurities on BP20 Components BP20, 1.0 μm 1. Methanol 25m x 0.53 mm ID 2. Dichloromethane 40°C, 2 min 3. Ethanol 10 °C/min 4. Dioxane impurity 120 °C 5. Dioxane FID, 280 °C Split, 30:1, Phase: Hydrogen, 2 psi Column: 0.2 μL Initial Temp.: 054448 Rate: Final Temp.: BPX5, 1.0 μm film Detector: neat Injector Mode: 60m x 0.25 mm ID Carrier Gas: 32 °C, 5 min. Injection Volume: 20 °C/min to 190 °C, Column Part Number.: 190°C, 2 min. FID Analysis of a Common Solvent Mixture on a Thick Film BPX5 360 °C He, 26.9 psi Components Phase: 1.9 mL/min. 1. Methanol Sample: On 2. Ethanol Column: 35 cm/sec at 40 °C 3. Acetone Split 4. Isopropyl alcohol Initial Temp: 100:1 5. Acetonitrile Rate 1: 0.3 μL 6. Dichloromethane Final Temp: 250 °C 7. 2-Butanone Detector Type: No 8. Ethyl acetate Detector Temp.: 4 mm ID Single Taper Liner 9. Benzene Carrier Gas: 092017 Carrier Gas Flow: 054123 Constant Flow: Average Linear Velocity: Injection Mode: Split Ratio: Injection Volume: Injection Temperature: Autosampler: Liner Type: Liner Part Number: Column Part Number: For further information on our full range of products, please visit www.sge.com 30

Pharmaceutical ANALYSIS OF Common pharmaceutical solvent on BPX-Volatiles Components 12. Ethyl acetate Phase: BPX-Volatiles, 1.4 μm film 1. Methanol 13. 2-Butanol Sample: 200 ppm in methanol 2. Ethanol 14. Tetrahydrofuran Column: 30 m x 0.25 mm ID 3. Ethyl ether 15. Chloroform 4. Acetone 16. Cyclohexane Initial Temp: 35 °C, 15 min 5. iso-propyl alcohol 17. Benzene Rate 1: 5 °C/min to 100 °C 6. Acetonitrile 18. n-Heptane Final Temp: 100 °C, 2 min 7. Methylene chloride 19. n-Butanol Detector Type: Mass Spectrometer 8. t-Butanol 20. 1,4-Dioxane Carrier Gas: He, 25.7 psi 9. Hexane 21. Pyridine Carrier Gas Flow: 1.8 mL/min 10. Propanol 22. Toluene Constant Flow: On 11. 2-Butanone 23. Dimethylformamide Average Linear Velocity: 35 cm/sec at 35 °C Injection Mode: Split Split Ratio: 100:1 Injection Volume: 0.5 μL Injection Temp: 250 °C Autosampler: No Liner Type: 4 mm ID Single Taper Liner Liner Part Number: 092017 Column Part No: 054980 Full Scan / SIM: Full scan 25-450 ANALYSIS OF Common pharmaceutical solvent on BPX-VOLATILES Components 32. Chlorobenzene Phase: BPX-Volatiles 1.4 μm film 1. Dichlorodifluoromethane 33. Ethylbenzene USEPA 502.2 mix: 200 ppm in Methanol 2. Chloromethane 34. Column: 30 m x 0.25 mm ID 3. Vinyl chloride 1,1,1,2-Tetrachloroethane 4. Bromomethane 35. m-Xylene Initial Temp: 40 °C, 0 min 5. Chloroethane 36. p-Xylene Rate 1: 6 °C to 210 °C 6. Trichlorofluoromethane 37. o-Xylene Rate 2: 15 °C to 240 °C 7. 1,1-Dichloroethene 38. Styrene Final Temp: 240 °C, 5 min 8. Dichloromethane 39. Bromoform Detector Type: Mass Spectrometer 9. trans-1,2-Dichloroethene 40. Isopropylbenzene Carrier Gas: He, 22.8 10. 1,1-Dichloroethane 41. Bromobenzene Carrier Gas Flow: 1.3 mL/min 11. 2,2-Dichloropropane 42. Constant Flow: On 12. cis-1,2-Dichloroethene 1,1,2,2-Tetrachloroethane Average Linear Velocity: 35 cm/sec at 40 °C 13. Bromochloromethane 43. 1,2,3-Trichloropropane Injection Mode: Split 14. Chloroform 44. n-Propyl benzene Split Ratio: 50:1 15. 1,1,1-Trichloroethane 45. 2-Chlorotoluene Injection Volume: 1 mL 16. 1,1-Dichloropropene 46. 1,3,5-Trimethylbenzene Injection Temp: 250 °C 17. Carbon tetrachloride 47. 4-Chlorotoluene Autosampler: No 18. Benzene 48. tert-Butylbenzene Liner Type: 4mm ID Single Taper Liner 19. 1,2-Dichloroethane 49. 1,2,4-Trimethylbenzene Liner Part No: 092017 20. Trichloroethene 50. sec-Butylbenzene Column Part No: 054980 21. 1,2-Dichloropropane 51. 1,3-Dichlorobenzene Full Scan / SIM: Full scan 45-450 22. Dibromomethane 52. p-Isopropyltoluene 23. Bromodichloromethane 53. 1,2-Dichlorobenzene 24. cis-1,3-Dichloropropene 54. n-Butylbenzene 25. Toluene 55. 1,4-Dichlorobenzene 26. trans-1,3- 56. 1,2-Dibromo-3- Dichloropropene chloropropane 27. 1,1,2-Trichloroethane 57. 1,2,4-Trichlorobenzene 28. Tetrachloroethene 58. Hexachlorobutadiene 29. 1,3-Dichloropropane 59. Naphthalene 30. Dibromochloromethane 60. 1,2,3-Trichlorobenzene 31. 1,2-Dibromoethane 31 For further information on our full range of products, please visit www.sge.com

Pharmaceutical Pharmaceutical ANALYSIS OF Class i solvents on bpx-volatiles Components Phase: BPX-Volatiles 1.4 μm film 1. 1,1-Dichloroethene Sample: 200 ppm in methanol 2. 1,1,1-Trichloroethane Column: 30 m x 0.25 mm ID 3. Carbon tetrachloride 4. Benzene Initial Temp: 40 °C, 1 min 5. 1,2-Dichloroethane Rate 1: 6 °C/min to 80 °C Final Temp: 80 °C Detector Type: Mass Spectrometer Carrier Gas: He, 6.7 psi Carrier Gas Flow: 0.9 mL/min Constant Flow: On Average Linear Velocity: 35 cm/sec at 50 °C Injection Mode: Split Split Ratio: 100:1 Injection Volume: 0.4 μL Injection Temp: 250 °C Autosampler: No Liner Type: 4 mm ID Single Taper Liner Liner Part Number: 092017 Column Part No: 054980 Full Scan / SIM: Full scan 30-450 ANALYSIS OF THE separation of THE CLASS III SOLVENTS ON BPX-VOLATILES Components 14. iso-Butanol Phase: BPX-Volatiles 1.4 μm film 1. Pentene 15. sec-Butanol Sample: 200 ppm in methanol 2. Ethanol 16. iso-Propyl acetate Column: 30 m x 0.25 mm ID 3. Ethyl ether 17. Heptane 4. Acetone 18. Acetic acid Initial Temp: 50 °C, 5 min 5. iso-Propyl alcohol 19. n-Butanol Rate 1: 10 °C/min to 85 °C, 1 min 6. Ethyl formate 20. Propyl acetate Rate 2: 15 °C/min to 170 °C, 7. Methyl acetate 21. 4-Methyl-2-pentanone Final Temp: 170 °C 8. Dichloromethane 22. Iso-Amyl alcohol Detector Type: Mass Spectrometer 9. Methylt-butyl ether 23. Iso-Butyl acetate Carrier Gas: He, 6.7 psi 10. n-Propanol 24. n-Amyl alcohol Carrier Gas Flow: 0.9 mL/min 11. Ethyl acetate 25. Butyl acetate Constant Flow: On 12.2-Butanone (MEK) 26. Dimethyl sulfoxide Average Linear Velocity: 35 cm/sec at 50 °C 13. Tetrahydrofuran Injection Mode: Split Split Ratio: 100:1 Injection Volume: 0.4 μL Injection Temp: 250 °C Autosampler: No Liner Type: 4 mm ID Single Taper Liner Liner Part Number: 092017 Column Part No: 054980 For further information on our full range of products, please visit www.sge.com 32

FFoorerennssicic Forensic and Toxicology analyses face similar challenges as those found in pharmaceutical assays. These methods are often very challenging due to the analysis of very active compounds as well as coming from samples that are detrimental to GC systems. These compounds are generally basic in nature that makes inertness of the system components critical to successful determinations. The robustness is another critical aspect of clinical analyses. The natures of sample compounds and matrices are extremely arduous on the analytical system. SGE’s columns are designed to withstand these ordeals and provide excellent lifetimes in difficult analyses. Applications • Analysis of Acid/Neutral Drugs on BPX35 • Analysis of Basic Drugs on BPX35 • Analysis of Underivatized Barbiturates on BP5 • Analysis of Various Drugs on BPX50 • Analysis of a Variety of Antidepressant and Anticonvulsant Drugs on BPX50 33 For further information on our full range of products, please visit www.sge.com

Forensic Analysis of Acid/Neutral Drugs on BPX35 Components BPX35, 0.25 μm 1. Ethosuximide 25 m x 0.22 mm ID 2. Barbital 3. Aprobarbital 100 °C, 1min 4. Butabarbital 10 °C/min 5. Amobarbital 300 °C, 5 min 6. Pentabarbital He, 150 kpa 7. Secobarbital Split, ( 20:1 ) 8. Meprobamate FID, 380 °C 9. Carisoprodal 054711 10. Glutethimide 11. Phenobarbital 12. Methaqualone 13. Primidone ACID/NEUTRAL DRUGS Phase: Column: Initial Temp: Rate: Final Temp: Carrier Gas: Injection Mode: Detector: Column Part Number: Analysis of Basic Drugs on BPX35 Components 7. Amitriptyline (Concentration: 8. Tetracaine 100-200 ug/ml) 9. Pyrilamine 1. Benzocaine 10. Unknown 2 Unknown 11. Diazepam 3. Meperidine 12. Flurazepam 4. Diphenhydramine 13. Papaverine 5. Lidocaine 14. Triazolam 6. Tripelennamine BASIC DRUGS BPX35, 0.25 μm Phase: 25m x 0.22 mm ID Column: 100 °C Initial Temp: 5 °C/min Rate: 325 °C, 5 min. Final Temp.: Helium 150 kpa Carrier Gas: Split, 0.5 μL (20:1) Injection Mode: FID, 380 °C Detector: 054711 Column Part Number: For further information on our full range of products, please visit www.sge.com 34

Forensic Analysis of Underivatized Barbiturates on BP5 Components UNDERIVATIZED BARBITURATES BP5, 1.0 μm 1. Barbital Phase: 12m x 0.53 mm I.D. 2. Butabarbital Column: 3. Amobarbital 195 °C 4. Pentabarbital Temp: Hydrogen 5. Secobarbital Carrier Gas: 10 mL/min 6. Hexabarbital Carrier Flow: 0.1 μL Injection Volume: 054197 Column Part Number: Analysis of Various Drugs on BPX50 Components 8. Methadone Phase: BPX50, 0.25 μm film 1. N,N-Dimethylaniline 9. N,N-Dimethylstearamide Sample: 5-10 ppm in methanol 2. Benzothiazole 10. Chloroquine Column: 30 m x 0.25 mm ID 3. Selegeline 11. Dextromoramide 4. Pethidine 12. Sitosterol Initial Temp: 150 °C, 0.5 min. 5. Unknown 13. Buspirone Rate 1: 10 °C/min to 180 °C, 6. a-octadecene Rate 2: 1.5 °C/min to 220 °C 7. Octadecanoic acid Rate 2: 30 °C/min to 260 °C Final Temp: 260 °C, 5 min. butyl ester Detector Type: FID Detector Temp.: 320 °C Carrier Gas: He, 25.7 psi Carrier Gas Flow: 1.8 mL/min. Constant Flow: On Average Linear Velocity: 35 cm/sec at 40 °C Injection Mode: Splitless Purge on Time: 0.5 min Purge on (Split) Vent Flow: 60 mL/min Injection Volume: 1 μL Injection Temperature: 250 °C Autosampler: No Liner Type: 4 mm ID Single Taper Liner Liner Part Number: 092017 Column Part Number: 054751 Full Scan / SIM: Full scan 45-450 35 For further information on our full range of products, please visit www.sge.com

Forensic Analysis of a Variety of Antidepressant and Anticonvulsant Drugs on BPX50 Components Phase: BPX50, 0.25 μm film 1. Benzothiazole Sample: 5-10 ppm in methanol 2. Selegeline Column: 30 m-x 0.25 mm ID 3. Methsuximide 4. Ketamine Initial Temp: 150 °C, 0.5 min. 5. Unknown Rate 1: 10 °C/min to 180 °C, 6. Brompheniramine Rate 2: 1.5 °C/min to 220 °C 7. Imipramine Rate 2: 30 °C/min to 260 °C 8. Mianserin Final Temp: 260 °C, 5 min. 9. Diothiepin Detector Type: FID 10. Carbamazepine Detector Temp.: 320 °C 11. Prazepam Carrier Gas: He, 25.7 psi 12. Diltiazem Carrier Gas Flow: 1.8 mL/min. 13. Pholcodine Constant Flow: On Average Linear Velocity: 35 cm/sec at 40 °C Injection Mode: Splitless Purge on Time: 0.5 min Purge on (Split) Vent Flow: 60 mL/min Injection Volume: 1 μL Injection Temperature: 250 °C Autosampler: No Liner Type: 4 mm ID Single Taper Liner Liner Part Number: 092017 Column Part Number: 054751 Full Scan / SIM: Full scan 45-450 FocusLiner™ improves reproducibility by: 4 mm ID liner Promoting uniform sample vaporization with quartz Maximizing sample vaporization on an inert surface wool in center of Acting as a particulate filter for dirty samples liner (incorrectly Improving injection reproducibility 10-fold positioned) Wiping needle tip during injection through fixed quartz wool Liner deactivated at high temperatures with wool in situ using SGE SGE’s high quality deactivation processes FocusLiner ™ (quartz wool correctly positioned) Acenaphytlene 2-Chlorophenol Decylamine Undecanol For further information on our full range of products, please visit www.sge.com 36

SGE Capillary Column Part Number Listing Phase Length ID Film Thickness Part No. Phase Length ID Film Thickness Part No. (m) (mm) (µm) (m) (mm) (µm) BP1 054022 SolGel-1ms 054793 BP1 10 0.1 0.1 054028 SolGel-1ms 60 0.25 0.25 054798 BP1 12 0.15 0.25 054029 SolGel-1ms 30 0.32 0.25 054794 BP1 25 0.15 0.25 054040 60 0.32 0.25 054167 BP1 12 0.22 0.1 054046 BP5 12 0.22 0.25 054168 BP1 12 0.22 0.25 054052 BP5 25 0.22 0.25 054171 BP1 12 0.22 054049 BP5 30 0.22 0.25 054169 BP1 15 0.22 1 054041 BP5 50 0.22 0.25 054175 BP1 25 0.22 0.25 054047 BP5 50 0.22 054182 BP1 25 0.22 0.1 054053 BP5 15 0.25 1 054183 BP1 25 0.22 0.25 054050 BP5 30 0.25 0.25 054203 BP1 30 0.22 054042 BP5 30 0.25 0.25 054184 BP1 50 0.22 1 054048 BP5 60 0.25 054215 BP1 50 0.22 0.25 054054 BP5 60 0.25 1 054179 BP1 50 0.22 0.1 054051 BP5 12 0.32 0.25 054176 BP1 60 0.22 0.25 054039 BP5 15 0.32 054180 BP1 15 0.25 054043 BP5 25 0.32 1 054186 BP1 15 0.25 1 054044 BP5 25 0.32 0.25 054192 BP1 30 0.25 0.25 054820 BP5 25 0.32 0.25 054177 BP1 30 0.25 0.1 054056 BP5 30 0.32 0.25 054216 BP1 30 0.25 0.25 054045 BP5 30 0.32 0.5 054189 BP1 60 0.25 0.25 054812 BP5 30 0.32 054187 BP1 60 0.25 0.5 054815 BP5 50 0.32 1 054193 BP1 60 0.25 054058 BP5 50 0.32 0.25 054178 BP1 12 0.32 1 054064 BP5 60 0.32 0.5 054188 BP1 12 0.32 0.25 054070 BP5 60 0.32 054197 BP1 12 0.32 0.5 054061 BP5 12 0.53 1 054194 BP1 15 0.32 054059 BP5 15 0.53 0.5 054199 BP1 25 0.32 1 054065 BP5 15 0.53 1 054198 BP1 25 0.32 0.25 054071 BP5 25 0.53 0.25 0541935 BP1 25 0.32 0.5 054076 BP5 30 0.53 1 054195 BP1 25 0.32 054081 BP5 30 0.53 1 054196 BP1 25 0.32 1 054062 BP5 30 0.53 1 054204 BP1 30 0.32 0.25 054068 BP5 60 0.53 1.5 054099 BP1 30 0.32 0.25 054813 BPX5 10 0.1 1 054106 BP1 30 0.32 0.5 054811 BPX5 10 0.15 0.5 054103 BP1 30 0.32 054073 BPX5 12 0.15 1 054107 BP1 30 0.32 1 054077 BPX5 12 0.15 5 054104 BP1 30 0.32 4 054060 BPX5 25 0.15 1.5 054108 BP1 50 0.32 5 054066 BPX5 25 0.15 0.1 054110 BP1 50 0.32 0.25 054072 BPX5 30 0.15 1.2 054105 BP1 50 0.32 0.5 054082 BPX5 50 0.15 0.25 054229 BP1 50 0.32 1 054067 BPX5 40 0.18 0.4 054112 BP1 60 0.32 1.5 054069 BPX5 12 0.22 0.25 054113 BP1 60 0.32 3 054810 BPX5 25 0.22 0.4 054116 BP1 60 0.32 4 054085 BPX5 25 0.22 0.15 054142 BP1 60 0.32 0.25 054086 BPX5 30 0.22 0.25 054114 BP1 12 0.53 0.5 054097 BPX5 50 0.22 0.18 054117 BP1 12 0.53 1 054870 BPX5 50 0.22 0.25 054149 BP1 15 0.53 5 054089 BPX5 7 0.25 0.25 0542170 BP1 15 0.53 0.25 054087 BPX5 15 0.25 1 054100 BP1 25 0.53 0.5 054098 BPX5 15 0.25 0.25 054121 BP1 25 0.53 1 054095 BPX5 15 0.25 0.25 0541011 BP1 25 0.53 5 054092 BPX5 30 0.25 1 054101 BP1 30 0.53 1 054090 BPX5 30 0.25 0.25 0541025 BP1 30 0.53 3 054819 BPX5 30 0.25 0.1 054122 BP1 30 0.53 0.5 054808 BPX5 30 0.25 0.25 054102 BP1 30 0.53 1 054806 BPX5 60 0.25 1 054123 BP1 30 0.53 1 054088 BPX5 60 0.25 0.1 0541261 BP1 50 0.53 3 054096 BPX5 6 0.32 0.25 054118 BP1 50 0.53 5 054871 BPX5 12 0.32 0.5 054124 BP1 60 0.53 0.5 054809 BPX5 12 0.32 1 054127 BP1 60 0.53 1 054807 BPX5 12 0.32 0.25 054144 BP1-PONA 60 0.53 2.6 054950 BPX5 15 0.32 1 054152 BP1-PONA 50 0.15 3 054818 BPX5 15 0.32 1 054119 BPX1 100 0.25 5 054777 BPX5 25 0.32 0.25 BPX1 10 0.1 1 0548025 0.5 054125 BPX1 6 0.53 5 054803 BPX5 25 0.32 1 054128 BPX1 10 0.53 0.5 054801 BPX5 25 0.32 0.25 BPX1 10 0.53 3 054802 1 054136 BPX1 Aluminum Clad 10 0.53 5 054800 BPX5 25 0.32 0.25 054145 BPX1 Aluminum Clad 5 0.53 0.5 054782 BPX5 30 0.32 0541205 BPX1 Aluminum Clad 5 0.53 0.5 054779 BPX5 30 0.32 0.5 054153 BPX1 10 0.53 0.1 054802 BPX5 30 0.32 1 054120 BPX1 Aluminum Clad 10 0.53 2.65 054800 BPX5 50 0.32 054126 BPX1 Aluminum Clad 5 0.53 0.1 054782 BPX5 50 0.32 3 054129 BPX1 Aluminum Clad 5 0.53 0.9 054779 BPX5 50 0.32 0.25 054146 SolGel-1ms 10 0.53 2.65 054795 BPX5 60 0.32 0.5 054154 30 0.25 0.1 BPX5 60 0.32 054133 0.17 BPX5 12 0.53 1 054130 0.1 BPX5 12 0.53 0.25 2.65 0.5 0.1 0.17 1 0.1 0.25 0.25 1 0.25 1 37 For further information on our full range of products, please visit www.sge.com

SGE Capillary Column Part Number Listing Phase Length ID Film Thickness Part No. Phase Length ID Film Thickness Part No. (m) (mm) (µm) (m) (mm) (µm) BPX5 054138 BP624 054839 BPX5 12 0.53 3 0541344 BP624 15 0.25 1.4 054840 BPX5 15 0.53 0.5 054147 BP624 30 0.25 1.4 054842 BPX5 15 0.53 1 0541347 BP624 60 0.25 1.4 054830 BPX5 15 0.53 1.5 054159 BP624 25 0.32 1.8 054832 BPX5 15 0.53 3 054134 BP624 30 0.32 1.8 054831 BPX5 25 0.53 0.25 054131 BP624 50 0.32 1.8 054841 BPX5 25 0.53 1 054139 BP624 60 0.32 1.8 054834 BPX5 25 0.53 3 0541345 BP624 25 0.53 3 054836 BPX5 30 0.53 0.5 054148 BP624 30 0.53 3 054835 BPX5 30 0.53 1 0541348 BP624 50 0.53 3 054838 BPX5 30 0.53 1.5 054160 BPX-Volatiles 60 0.53 3 054978 BPX5 30 0.53 3 054132 BPX-Volatiles 20 0.18 1 054979 50 0.53 1 BPX-Volatiles 40 0.18 1 054980 BPX5 054158 BPX-Volatiles 30 0.25 1.4 054981 HT5 60 0.53 1 054631 BPX-Volatiles 60 0.25 1.4 054982 12 0.22 0.1 BPX-Volatiles 30 0.32 1.8 054983 HT5 054632 BPX-Volatiles 60 0.32 1.8 054984 HT5 25 0.22 0.1 054633 BPX-Volatiles 054985 HT5 15 0.25 0.1 054634 BP10 30 0.53 3 054252 HT5 30 0.25 0.1 054641 BP10 60 0.53 3 054253 HT5 12 0.32 0.1 054667 BP10 12 0.22 0.25 054254 HT5 15 0.32 0.5 054642 BP10 25 0.22 0.25 054255 HT5 25 0.32 0.1 054668 BP10 50 0.22 0.25 054256 HT5 30 0.32 0.5 054655 BP10 15 0.25 0.25 054271 HT5 6 0.53 0.1 054670 BP10 30 0.25 0.25 054257 HT5 10 0.53 0.5 054657 BP10 30 0.25 1 054258 HT5 12 0.53 0.15 054671 BP10 60 0.25 0.25 054264 HT5 15 0.53 0.5 054658 BP10 15 0.32 0.25 054262 HT5 25 0.53 0.15 054672 BP10 15 0.32 0.5 054268 HT5 Aluminum Clad 30 0.53 0.5 054635 BP10 25 0.32 0.25 054259 HT5 Aluminum Clad 12 0.22 0.1 054636 BP10 25 0.32 0.5 054265 HT5 Aluminum Clad 25 0.22 0.1 054651 BP10 30 0.32 0.25 054270 HT5 Aluminum Clad 12 0.32 0.1 054652 BP10 30 0.32 0.5 054269 HT5 Aluminum Clad 25 0.32 0.1 054653 BP10 30 0.32 1 054260 HT5 Aluminum Clad 50 0.32 0.1 054673 BP10 50 0.32 0.5 054266 HT5 Aluminum Clad 5 0.53 0.075 054661 BP10 60 0.32 0.25 054282 HT5 Aluminum Clad 6 0.53 0.1 054662 BP10 60 0.32 0.5 054280 HT5 Aluminum Clad 12 0.53 0.15 054665 BP10 15 0.53 1 054283 HT8 25 0.53 0.15 054690 BPX50 25 0.53 1 054739 HT8 10 0.1 0.1 054686 BPX50 30 0.53 1 054738 HT8 40 0.18 Proprietary 054674 BPX50 10 0.1 0.05 054740 HT8 12 0.22 0.25 054675 BPX50 10 0.1 0.07 054741 HT8 25 0.22 0.25 054676 BPX50 10 0.1 0.1 054750 HT8 50 0.22 0.25 054677 BPX50 30 0.15 0.15 054751 HT8 30 0.25 0.25 054683 BPX50 15 0.25 0.25 054752 HT8 60 0.25 0.25 054679 BPX50 30 0.25 0.25 054760 HT8 12 0.32 0.25 054680 BPX50 60 0.25 0.25 054761 HT8 25 0.32 0.25 054681 BPX50 15 0.32 0.25 054762 HT8 50 0.32 0.25 054682 BPX50 30 0.32 0.25 054770 HT8 60 0.32 0.25 054684 BPX50 60 0.32 0.25 054771 HT8 12 0.53 0.5 054685 BPX50 15 0.53 0.5 054772 BPX35 25 0.53 0.5 054699 BP225 30 0.53 0.5 054352 BPX35 10 0.1 0.1 054713 BP225 30 0.53 1 054353 BPX35 15 0.22 0.25 054711 BP225 25 0.22 0.25 054358 BPX35 25 0.22 0.25 054714 BP225 50 0.22 0.25 054364 BPX35 30 0.22 0.25 054712 BP20 25 0.32 0.25 054405 BPX35 50 0.22 0.25 054700 BP20 25 0.53 0.5 054420 BPX35 15 0.25 0.25 054703 BP20 10 0.1 0.1 054421 BPX35 15 0.25 1 054701 BP20 12 0.22 0.25 054424 BPX35 30 0.25 0.25 0547025 BP20 25 0.22 0.25 054422 BPX35 30 0.25 0.5 054704 BP20 30 0.22 0.25 054425 BPX35 30 0.25 1 054702 BP20 50 0.22 0.25 054426 BPX35 60 0.25 0.25 054705 BP20 60 0.22 0.25 054427 BPX35 60 0.25 1 054723 BP20 15 0.25 0.25 054415 BPX35 15 0.32 0.25 054718 BP20 30 0.25 0.25 054439 BPX35 15 0.32 0.5 054716 BP20 30 0.25 0.5 054428 BPX35 15 0.32 1 054721 BP20 30 0.25 1 054458 BPX35 25 0.32 0.25 054724 BP20 60 0.25 0.25 054432 BPX35 30 0.32 0.25 0547158 BP20 60 0.25 0.5 054430 BPX35 30 0.32 0.5 054717 BP20 15 0.32 0.25 054436 BPX35 30 0.32 1 054722 BP20 25 0.32 0.25 054442 BPX35 50 0.32 0.25 054725 BP20 25 0.32 0.5 054433 BPX35 60 0.32 0.25 054734 BP20 25 0.32 1 054438 BPX35 15 0.53 0.5 054736 BP20 30 0.32 0.25 054444 BPX35 15 0.53 1 054735 BP20 30 0.32 0.5 054431 BPX35 30 0.53 0.5 054737 BP20 30 0.32 1 054437 BPX608 30 0.53 1 054823 BP20 50 0.32 0.25 054443 BP624 25 0.32 0.4 054826 50 0.32 0.5 BP624 25 0.22 1.2 054827 50 0.32 1 30 0.22 1.2 For further information on our full range of products, please visit www.sge.com 38

SGE Capillary Column Part Number Listing Phase Length ID Film Thickness Part No. Phase Length ID Film Thickness Part No. (m) (mm) (µm) (m) (mm) (µm) BP20 60 0.32 0.25 054434 BP21 30 0.53 0.5 054477 BP20 60 0.32 0.5 054457 BP21 30 0.53 1 054478 BP20 60 0.32 1 054445 BPX70 10 0.2 054600 BP20 12 0.53 1 054447 BPX70 12 0.1 0.25 054601 BP20 12 0.53 2 054455 BPX70 25 0.22 0.25 054602 BP20 15 0.53 0.5 054961 BPX70 30 0.22 0.25 054612 BP20 15 0.53 1 054450 BPX70 50 0.22 0.25 054603 BP20 25 0.53 1 054448 BPX70 60 0.22 0.25 054613 BP20 25 0.53 2 054456 BPX70 15 0.22 0.25 054621 BP20 30 0.53 0.5 054440 BPX70 30 0.25 0.25 054622 BP20 30 0.53 1 054451 BPX70 0.25 054623 BP20 60 0.53 0.5 054963 BPX70 60 0.25 054624 BP20 60 0.53 1 0544515 BPX70 120 0.25 0.25 054605 SolGel-WAX™ 30 0.25 0.25 054796 BPX70 12 0.25 0.25 054606 SolGel-WAX™ 30 0.25 1 054787 BP21 25 0.32 0.25 054469 SolGel-WAX™ 60 0.25 0.25 054791 BP21 50 0.32 0.25 054472 SolGel-WAX™ 30 0.32 0.25 054788 BP21 0.32 054473 SolGel-WAX™ 30 0.32 0.5 054797 BP21 60 0.25 054476 SolGel-WAX™ 60 0.32 0.25 054789 BP21 12 0.32 0.5 054474 SolGel-WAX™ 054792 BP21 15 0.53 0.5 054477 SolGel-WAX™ 60 0.32 0.5 054786 BP21 25 0.53 0.5 054478 SolGel-WAX™ 30 0.53 0.5 054785 BPX70 30 0.53 0.5 054600 BP21 30 0.53 1 054462 BPX70 30 0.53 1 054601 BP21 25 0.22 0.25 054463 BPX70 10 0.53 0.2 054602 BP21 50 0.22 0.25 054464 BPX70 12 0.1 0.25 054612 BP21 15 0.25 0.25 054465 BPX70 25 0.22 0.25 054603 BP21 30 0.25 0.25 054466 BPX70 30 0.22 0.25 054613 BP21 60 0.25 0.25 054467 BPX70 0.22 054621 BP21 12 0.32 0.25 054470 BPX70 50 0.25 054622 BP21 15 0.32 0.25 054468 BPX70 60 0.22 0.25 054623 BP21 25 0.32 0.25 054471 BPX70 15 0.22 0.25 054624 BP21 30 0.32 0.25 054469 BPX70 30 0.25 0.25 054605 BP21 50 0.32 0.25 054472 BPX70 60 0.25 0.25 054606 BP21 60 0.32 0.25 054473 BPX90 120 0.25 0.25 054570 BP21 12 0.53 0.5 054476 BPX90 12 0.25 0.25 054573 BP21 15 0.53 0.5 054474 BPX90 25 0.32 0.25 054580 BP21 25 0.53 0.5 054477 BPX90 15 0.32 0.25 054590 BP21 30 0.53 0.5 054478 BPX90 15 0.25 0.50 05493 BP21 30 0.53 1 054474 BPX90 30 0.32 0.25 054980 25 0.53 0.5 60 0.25 0.25 60 0.25 0.50 30 0.32 0.25 0.25 GC Column Replacement Guide Column to Replace Description SGE Phase 100% Dimethyl Polysiloxane BP1 DB-1, HP-1, Ultra-1, SPB-1, CP-Sil 5CB, RSL-150, RSL-160, Rtx®-1, ZB-1, CB-1, OV®-1, PE-1, 007-1(MS), SP-2100, SE-30, RH-1, CC-1, CP-Sil 5CB MS, VF-1ms, Petrocol DH Unique highly inert phase SolGel + 100% Dimethyl Polysiloxane SolGel-1ms™ DB-HT Sim Dis, DB-2887, Rtx-2887, HP-1, Petrocol 2887, Petrocol EX2887 100% Dimethyl Polysiloxane BPX1 Petrocol DH, DB-Petro 100% Dimethyl Polysiloxane BP1-PONA DB-5, DB-5.625, Rtx-5, HP-5, Ultra-2, PTE-5, PB-5, MDN-5, CP-Sil 8CB, VB-5 & ZB-5 5% Phenyl Polysiloxane BP5 DB-5, DB-5ms, HP-5, Ultra-2, Rtx®-5, Rtx-5Sil MS, Rtx 5MS, AT-5, AT-5MS, 007-5MS, 5% Phenyl Polysilphenylene-siloxane BPX5 SPB-5,CP-Sil 8CB, VF-5ms, RSL-200, CB-5, OV®-5, PE-5, 007-2(MPS-5), SE-52, SE-54, XTI-5, PTE-5, CC-5, RH-5ms, ZB-5 5% Phenyl Polycarborane-siloxane HT5 MXT-1 SimDist, HT-SimDist, DistCB, MXT-500 8% Phenyl Polycarborane-siloxane HT8 No equivalent, unique high temperature column with special selectivity 35% Phenyl Polysilphenylene-siloxane BPX35 DB-35, DB-35ms, Rtx-35, HP-35, HP-35MS, SPB-35, MDN-35, VB-50, ZB-35 35% Phenyl Polysilphenylene-siloxane BPX608 DB-608, Rtx-35, SPB-608 50% Phenyl Polysilphenylene-siloxane BPX50 OV-17, SP-2250, DB-17ms, DB-17ht, Rtx-50, SPB-50, HP-50+, HP-17, VB-50/608, ZB-50 70% Cyanopropyl Polysilphenylene-siloxane BPX70 DB-23, CP-Sil 88, VF-23ms, SP-2330, SP-2380, Rtx®-2330, 007-23, AT-Silar, PE-23 SolGel + Polyethylene Glycol SolGel-WAX™ Unique highly inert phase DB-Wax, Rtx-Wax, Stabilwax, HP20M, HP-Wax, HP-INNOWax, Supelcowax-10, AT-Wax, Nukol, Polyethylene Glycol BP20 (Wax) CP Wax 2CB, VB-WAX, ZB-WAX DB-FFAP, HP-FFAP, Stabilwax-DA, CP Wax 58CB, VB-FFAP, ZB-FFAP Polyethylene Glycol (TPA treated) BP21 (FFAP) DB-1701, Rtx-1701, HP-1701, SPB-7, CP-Sil 19CB, VB-1701, ZB-1701 14% Cyanopropylphenyl Polysiloxane BP10 (1701) HP-225, DB-225, Rtx-225 50% Cyanopropylphenyl Polysiloxane BP225 DB-624, HP-VOC, Rtx Volatiles, VOCOL, VB-624, ZB-624 Cyanopropylphenyl Polysiloxane BP624, BPX-Volatiles Cyclodex-B, Rt-BDEXm Permethylated Beta Cyclodextrin CYDEX-B Unique highly polar phase 90% Cynopropyl Polysilphenylene-siloxane BPX90 39 For further information on our full range of products, please visit www.sge.com

GC Troubleshooting and Maintenance Symptom Possible cause Solution High baseline level Septum bleed and/or contaminated liner Replace septa and insert a new inlet liner. Ensure gas traps are installed correctly to remove moisture, organics and oxygen. Poor carrier gas quality causing phase decomposition Ensure high purity carrier gas is used. Lower maximum program temperature. Neutralize sample before injecting Filter sample. Use a FocusLiner™. Maximum temperature of the phase has been exceeded Dilute sample. Use a high purity solvent. Highly acid or alkaline samples Increase the plunger depression speed. Change to a single solvent Dirty samples Can happen with some pesticides. Lower injector temperature. Recondition capillary column and re-inject sample. Reposition column according to manufacturer’s instructions. Dilute sample. Contaminated solvent Use a thicker film. Increase the split ratio. Split peaks Poor manual injection technique Cut 50cm off the front of the column and re-install in the injector. Recondition column. Mixed solvent Choose a more polar column. Replace with a new fully deactivated inlet liner. Compound degradation Cut 5cm off the front of the column and re-install in the injector Increase make up gas flow according to manufacturer’s instructions. Check carrier gas flow. Increase split flow or use the ‘solvent effect’ to focus peaks. Column inserted too far into injector Cut 50cm off the front of the column and re-install in the injector. Change column polarity or lower temp. program ramp rate to separate peaks. Fronting peaks Too much sample injected on to column Check injector conditions are reproducible e.g. temperature and split ratio. Increase sampling rate or reduce number of ions detected in SIM mode. Tighten septum cap or replace with new septum. Verify carrier gas flow rate. Check inlet pressure on GC. Ensure pressure in gas line and gas cylinder is OK Tailing peaks Column contamination Re-check temperature method conditions Re-check dimensions on column tag against column description. Dilute sample or increase split ratio. Sample not suitable for phase polarity Cut 50cm off the front of the column and re-install in the injector Poorly deactivated inlet liner Recondition column. Replace column. Graphite ferrule contamination in the start of the column Re-check temperature method conditions Broad peaks Make-up gas flow rate for atmospheric detectors is low Re-check carrier gas velocity and optimize to Van Deemter optima. Cut 50cm off the front of the column and re-install in the injector. Carrier gas flow is low Ensure technique is consistent. Split gas flow is too low Check all connections from GC to computer / integrator. Check injector number is consistent with detector number being monitored. Column contamination If using water, reduce injection volume. Water can extinguish the flame. Check flame gas pressures Co-elution of peaks Use a known good syringe to confirm this is the problem. Check all column and injector connections. Change in sample concentration Check column for breakage. This can sometimes be difficult to locate as the fused silica can break leaving the polyimide outer coating intact. Mass spectrometer sampling rate is too low causing Cut 5cm off the front and back ends of the column and re-install. Re-confirm with a known standard concentration injection. triangular-looking peaks Check flame gas pressures Replace inlet liner with a new deactivated liner. Shifting Leaking septum Cut 50cm off the front of the column, re-install in the injector and re-condition the column retention times Carrier gas velocity has changed Re-check solvent and method temperatures. Ensure syringe has been thoroughly washed with solvent between injections. Sometimes this can involve 20 solvent rinses. Confirm by using a different source of primary standards. Method temperature has changed Use a different type of solvent or confirm by using a different source of the same solvent. Column dimensions and film thickness have changed after Can appear as discrete peaks in temperature program runs. Will disappear with isothermal analysis. Replace septum. Could also be from sample vial septa installing a new column Peaks will appear broader for that part of the chromatogram. Confirm by extending run time and ensuring all peaks have eluted from sample. Sample concentration has changed – more has been Inject twice the amount of pure solvent (this may need to be repeated). Carrier gas lines may also need to be cleaned injected on column Dirty column. Extra non-volatile material deposited on the column has caused a change in column polarity Loss of Aging column has resulted in a substantial loss of phase peak resolution causing a loss in column resolving power Method temperature has changed Carrier gas velocity has changed Dirty column. Extra non-volatile material deposited on the column has caused a change in column polarity Manual injection technique or operator has changed No peaks GC incorrectly wired Wrong detector is being monitored FID flame is out Syringe is blocked or leaking around plunger Massive leak in system Column is blocked Loss of sensitivity Concentration of sample has changed Flame gas flow rates and/or make-up gas flow have changed System has become active Splitless conditions have changed Ghost peaks Syringe has become contaminated from previous sample New standards have impurities Impurities in solvent Septum bleed Peaks are still eluting from previous run Flashback has happened For further information on our full range of products, please visit www.sge.com 40

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