PALL eBDs Sample set/Oxygen Analyzer • Sterile weld platelet component to set • Fill pouch with ~3 mL of product • Disconnect sample pouch from set and incubate at 35°C for 24-30 hrs • Measure the O2 content in the air above the plasma sample with insertion of analyzer probe into pouch • LED display will read PASS or FAIL SCACM Conference Jan. 20, 2009
Aliquoting platelet sample for bacterial detection testing SCACM Conference Jan. 20, 2009
Inoculating culture bottle SCACM Conference Jan. 20, 2009
New FDA approved device for transfusion services The Abbott Verax Platelet PGD® Test • A rapid, qualitative immunoassay for the detection of Aerobic and Anaerobic; Gram-positive and Gram-negative bacteria in leukocyte reduced apheresis platelets (SDP) • Approx. 30 minute TAT • SDP must have undergone QC culture by blood supplier!! SCACM Conference Jan. 20, 2009
Verax rapid Platelet PGD® Test Single-use disposable test 1. Rapid: approx. 25 mins (3 mins hand on) 2. Positive typically < 10 minutes 3. Sensitivity approx. 103 CFU/mL 4. Shared 500 uL sample addition well Sample Well Gram-Positive Gram-Negative Read Window Read Window SCACM Conference Jan. 20, 2009
Comparison of three principles Technology Verax PGD BacT/ALERT Pall eBDS Sample Volume Conserved Culture Aerobic culture Bacterial Ag CO2 Measure O2 measure Time to Result Immunoassay 3-5 mL Detect Aerobic 4-20 mL and Anaerobic 500 uL 24 – 30 hrs bacteria? 24 – 96 hrs 10 – 30 min (positives typically within 10 min) Yes Yes, but time varies Misses anaerobes Clinical Specificity 99.7% 99.2-99.8% ~ 99% SCACM Conference Jan. 20, 56 2009
Storage and transportation “Blood cold chain” A system for storing and transporting blood and blood products, within the correct temperature range and conditions, from the point of collection from blood donors to the point of transfusion to the patient. http://www.who.int/bloodsafety/processing/cold_chain/en/
58 https://www.bmedicalsystems.com/en/products/blood-safety/blood-cold-chain/
Storage transportation and shelf-life of blood components Component Storage Transportation Shelf-life Whole blood 1-6 oC* 1-10 oC - ACD, CPD, CP2D (21 days) - CPDA-1 (35 days) Irradiated WB 1-6 oC 1-10 oC 28 days after irradiation or before as dated Red blood cells 1-6 oC 1-10 oC - ACD, CPD, CP2D (21 days) (RBCs) - CPDA-1 (35 days) *inwwcwa.bsleoooddfonpaltaiotnethlaei.tcopmreparation: 20-24 oC
Storage transportation and shelf-life of blood components Component Storage Transportation Shelf-life Frozen RBCs In freezing 10 years condition (In case of >10 years the policy must be implemented) 40% Glycerol ≤ 65 oC 20% Glycerol ≤ 120 oC Irradiated 1-6 oC 1-10 oC 28 days after irradiation or RBCs before as dated LDRC 1-6 oC 1-10 oC - ACD, CPD, CP2D--21 days - CPDA-1--35 days - Additive solution 42 days - Open system 24 hrs Washed RBCs 1-6 oC 1-10 oC 24 hrs www.blooddonationthai.com
Storage transportation and shelf-life of blood components Component Storage Transportation Shelf-life Platelets (all) 20-24 oC* 20-24 oC 4 hrs** (open systrm) Irradiated 20-24 oC* 5 days (closed system) platelets LDPC 20-24 oC* 20-24 oC* 4 hrs** (open systrm) (open system) 5 days (closed system) Pooled LPPC 20-24 oC* 4 hrs 5 days*** (closed system and kept in specified plastic bag www.blo*odadolnla-ttioimnthaei.caomgitate ** if agitate, 24 hrs *** if no agitation, 24 hrs
Storage transportation and shelf-life of blood components Component Storage Transportation Shelf-life FFP ≤ -18 oC ≤ -65 oC In freezing 12 months (≤-18 oC) Thawed FFP 1-6 oC condition 7 years (≤-65 oC) Cryoprecipitate ≤ -18 oC AHF 1-10 oC 24 hrs In freezing 12 months after condition phlebotomy Thawed 20-24 oC 20-24 oC 4 hrs (pooled or open cryoprecipitate system) AHF 6 hrs (single unit) www.blooddonationthai.com
Factors affecting the quality of blood components 1. Selection of donor 2. Quality of blood bag and anticoagulant preservative solution used 3. Techniques of phlebotomy • Clean venipuncture • Minimal tissue trauma • Flow-continuous and uninterrupted and should be completed in 8-10 min • Frequent gentle mixing
Factors affecting the quality of blood components 4. Time period: 5. Transit temperature 6. Refrigerated centrifuge calibration for maximum yield in minimum time • Critical variables – speed, temp, duration, rotor size • Accurate balancing – dry weight preferred • Bag position • Swinging cups better than fixed angle cups 7. Storage temperature 8. Uninterrupted, gentle flat bedded platelet incubator/agitator
Suggest readings 1. Roback JD, editor: Technical Manual, 17th ed, Bethesda, Md, 2011, AABB. 2. วิทยาศาสตรกH ารบรกิ ารโลหติ เลOม 2, 2558 3. มาตรฐานธนาคารเลอื ดและงานบริการโลหติ , ศนู ยHบริการโลหติ แหOงชาติ สภากาชาดไทย, พมิ พคH ร้งั ท่ี 4 พ.ศ. 2558 4. การใชโi ลหิตและสวO นประกอบโลหติ อยOางเหมาะสม, ศนู ยบH รกิ ารโลหติ แหOงชาติ สภากาชาดไทย, พ.ศ. 2554 37
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