Basic Biochemistry_Course Outline

BASIC BIOCHEMISTRY YEAR ONE: SEMESTER 1 MLT 103: BASIC BIOCHEMISTRY The purpose of this course is to enable the student to understand the principles underlying body processes as a basis for recognizing, interpreting and correcting deviation from normal body functions. Course Objectives On completion of this course the students will be able to: 1. Explain biochemistry and its importance 2. Classify carbohydrates into aldoses and ketoses, and their various structural levels 3. Differentiate between saturated and unsaturated fatty acids 4. Enumerate the chemical structure, classification and functions of proteins 5. Differentiate the five classes of immunoglobulin based on the primary structure of their heavy chains 6. Explain the concept of enzymology 7. Explain Metabolism and metabolic processes 8. Explain the importance of glycolysis and pentose phosphate pathway 9. Describe Water, electrolyte and acid base balance, the mechanism of homeostasis Course Content  Introduction to Biochemistry; Historical background and definitions of biochemistry, Carbohydrate. Classification of carbohydrates into monosaccharide’s i.e. Aldoses and ketoses, oligosaccharides and polysaccharides. Immunoglobulins: Definition of immunoglobulins, structure of immunoglobulins, classification of Immunoglobulins.  Enzymes :Definition , Nomenclature ,Classification , Factors affecting enzyme activity, Active site, Coenzyme , Enzyme Inhibition, Units of enzyme , Isoenzymes , Enzyme pattern in diseases. Metabolism: Describe the metabolic processes in the human body. Water and electrolyte balance: Electrolytes distribution between the ECF and ICF compartments, movement of electrolytes in response to some metabolic cellular

conditions and their effect on volume, composition of body fluids, mechanism of sodium and potassium balance in the body.  Definition : acids, bases, pH, Molarity, Molality , Sources of acids in the body, major chemical buffer systems in the body, regulation of hydrogen and bicarbonate ion concentration in the blood. Mode of Delivery: Lectures, discussions, laboratory work and activities with role play by students. Recommended Reading Materials: Albert L., David L. N. and Michael M. C. (Jun 2008); Lehninger Principles of Biochemistry, 5th ed., W. H. Freeman ( Hardback), New York Lubert S. L. (1997); Biochemistry, 3rd ed., W.H. Freeman, New York Thomas M. D. (1997); Textbook of biochemistry-with clinical correlations, 4th ed., Wiley, New York Campbell P. N. and Smith A. D. (2000); Biochemistry Illustrated, 4th ed., Churchill Livingstone, Edinburg Matthew C. K., Van Holde K. E. and Ahern K. G. (2000); Biochemistry, 3rd ed., Benjamin Cummings, San Francisco. Lieberman, M. & Marks, A. D. (2009); Marks’ Basic Medical Biochemistry; A clinical Approach. Murray, Bender, Botham, Kennelly, Rodwell, Weil: Harper’s Illustrated Biochemistry (28th ed.) – McGraw Hill Publishers Robert, David, Kathleen, Peter Victor & P. Anthony (2009): Harper’s Illustrated Biochemistry Nigam, A., &Ayyagari, A. (2007): Lab Manual in Biochemistry, Immunology and Biotechnology Nelson, D. L. & Cox, M. M. (2004) – Lehninger Principles of Biochemistry. Harrison, K. (1965): A Guide-Book to Biochemistry