ILBS Annual Book Report 2017

4. Visiting faculty for the training of Lab Technicians on Blood Safety Training Programme at Department of Transfusion Medicine BMHRC, Bhopal from 04th to 08th September 2017. 5. Expert Faculty for “Training of Trainers for Strengthening of Blood services e-Rakt kosh” under NHM and NIB collaborative program at NIB Noida in July and September 2017. 6. Invited as an expert for two Day Workshop on Strengthening of Blood Services and Haemovigilance at Aizawl, Mizoram in August 2017. 133

Transfusion MedicineCardiology 134

Transfusion MedicineCardiology Cardiology The department of cardiology provides noninvasive cardiology services to outpatients and inpatients for a more complete patient evaluation and management. The department is also involved in research work in cardiology related issues in the liver disease patients. Services Rendered Services 2016 2017 Contrast Echo 550 456 DSE 692 518 Plain Echo 4252 4478 Tread Mill Test (TMT) 119 177 HOLTER 23t 19 ECG 3015 3494 STRESS ECHO 37 10 TOTAL 8688 10651 Dr. Arun Kumar Sood Staff He has recently joined Faculty Designation as a senior consultant Dr Arun Kumar Sood Senior Consultant in the department of Technical staff Cardiology. Mr Jitendra Kumar Singh, Mr Harshit Nurse Soni 135

Department Of Molecular Department Of Molecular and and Cellular Medicine Cellular Medicine 136

Department Of Molecular and Cellular Medicine Mission of the department is to “understand the molecular basis of liver disease and translating findings into clinical applications for better diagnosis, effective treatment and prevention strategies”. Our vision is to develop into a state of the art research centre of excellence in Liver Biology and foster strong collaboration between laboratory scientists, clinicians, and industry. The three pillars of the department are basic research, discovery research and translational science. In this regard, the department fosters multidisciplinary scientific research activities in modern areas of biology. A major focus is on translational approaches whereby research carried out in understanding liver diseases using animal models, in vitro systems and clinical specimens are translated for biomarker discovery. The Department has now extensively invested in development of state of the art molecular biology facility. In this regard ‘multi-omics’ technologies is being extensively used for understanding underlying causes and progression of liver and related diseases. The research in the department is funded dominantly by extramural grants from various national and international funding agencies. The main areas of interests include:  Mechanisms of immune paresis and sepsis in liver failure.  Epigenetics regulation of reactivation of Hepatitis B and liver cancer.  Relation of gut microbiome to liver injury .  Hepatic regeneration.  Bone marrow and hepatic stem cell biology.  Mechanisms of immune paresis and sepsis in liver failure.  Molecular pathways and therapeutic targets in Hepatocellular Cancers and identifying Hepatostats. 137

 “OMICS” including, proteomics, metagenomics and metabolomics in liver injury and regeneration.  Albumin biology and iron dysregulation in liver failure. National and International Recognitions:  ILBS-NIH laboratory of Molecular Immunology.  Metabolome and Metagenomics laboratory in partnership INSERM, France  DST funded core Laboratory for Non- Transplant Therapeutic Strategies For Advanced Liver Diseases.  ILBS-DST-FIST laboratory for Confocal Imaging supported by DST, Ministry of Science and Technology. Highlights of the Research Activities A brief overview of the various research works carried out are highlighted below: ƒ Circulating Endothelial Progenitor Cells indicate an inflammatory phenotype and function in patients with alcoholic liver cirrhosis (ALC) (Indo-Australian Project) (Dr. Nirupma Trehanpati, Dr. Savneet Kaur and Prof. S.K.Sarin) Given an important role of endothelial progenitor cells (EPCs) in liver injury and tissue repair, we have comprehensively characterized the phenotype and functions of EPCs in alcoholic The work revealed CD45int and CD45hi EPCs significantly increased in ALC patients compared to controls. CyTOF data further showed that CD45hi EPCs were distinct from CD45- and CD45int EPCs, with higher expression of T cell and myeloid markers, including CD3, CD4, HLA-DR, CD11c and chemokine receptors, CCR2, CCR5, CCR7 and CX3CR1. To summarize, it may be inferred that CD45hi EPCs have completely different immunophenotype profile compared to CD45- EPCs with increased T cell/myeloid markers and chemokine receptors. Further, high levels of circulating CD45hi EPCs and enhanced inflammatory cytokines and angiogenic mediators in patients suggest an inflammatory role of these cells in ALC. 138

ƒ MicroRNAs in liver disease (Dr. Nirupma Trehanpati, Dr Tanvi Agrawal, Dr Manish Chaudhary, Dr. Avishek, Dr. Vijay Kumar and Prof. S.K.Sarin).  Using a newly added high throughput RNA-miRNA-protein analyser (nano string) the group has recently discovered new miRNAs as biomarkers in Hepatitis C virus recurrence after liver transplantation. miRNA profiling using both liver tissue and plasma lead to identification of miRNAs which are modulated due to active viral replication. Nine miRNAs (miR-422a, miR-4516, miR-4454, 494-3p, miR-1283, 148a-3p, 199a-3p, 223- 3p and 26a-5p) were differentially expressed in pre-transplant plasma of patients who developed fibrosis after transplant compared to those who does not. Of these 9 miRNA, miR-494-3p and miR-422a were significantly upregulated in plasma as well as the liver tissue indicating their role as non-invasive markers differentiating patients who will develop disease recurrence after transplant.  Using high throughput microarray based applications the group has identified miRNA networks and their target genes in the liver of hepatitis B patients involved in HBV replication, liver injury and liver fibrosis. The work revealed that immune tolerant group showed elevated miR-199a-5p, miR-221-3p and Let-7a-3p levels which could target genes involved in innate immune response and viral replication. In acute viral hepatitis miR-125b- by effecting STAT3 pathway which emerged as a key player. In HBV early fibrosis and cirrhotic groups, micro RNAs identified had a role on chronic inflammation (Singh AK, et al, Hepatology, 2017). Fold change A B miRNA (Non reccurence vs Reccurence) hsa-miR-422a -63.57 hsa-miR-4516 -55.9 hsa-miR-4554+hsa-miR-7975 -18.3 hsa-miR-494-3p -8.67 hsa-miR-1283 -3.11 hsa-miR-148a-3p 1.83 hsa-miR-199a-3p+hsa-miR-199b-3p 5.14 hsa-miR-223-3p 6.27 hsa-miR-26a-5p 8.23 Fold change C miRNA (Non reccurence vs Reccurence) hsa-miR-494-3p -12.32 hsa-miR-422a -9.65 hsa-miR-4286 -5.72 hsa-miR-1307-3p 5.72 hsa-miR-216a-5p 6.26 Expression analysis of miRNA using Nanostring Technology showing differential regulation of miRNA profile in (A) HCV RNA positive tissue vs Negative tissue (B) In pre transplant liver tissue and (C) pre transplant plasma of patients who had non reccurence of disease vs recurrence of HCV related disease. 139

ƒ Hepato-Immunology in HBV transmission (Dr. Nirupma Trehanpati and Prof. S.K. Sarin)  Impaired immunity in Hepatitis B infected pregnant females enhances the chance of vertical transmission to newborns. The immunology group has focused on understanding the immune status of HBV positive mother who transmit HBV to their newborn and who do not do so. In brief the work showed HBV transmitting mothers had decreased frequencies and defective HBV specific DCs, CD4, CD8, TFh and B cell mediated protective responses. RNAseq showed decreased expression of CD4, CD8, B, and TFh genes in HBV transmiting mothers. The study for first time showed the serum IL-21 levels, TFh, and plasma B cells are useful predictors of vertical transmission of HBV from infected mothers to the newborns. In conclusion, defective maternal immunity influences HBV transmission (Vyas et al, 2017 manuscript under review in Hepatology and best paper award ISG Immune response in HBV vertical transmission. meeting, 2017). ƒ Liver Cancer (Dr. Nirupma Trehanpati, Dr.Gayatri Ramakrishna and Prof. S.K. Sarin)  miR-26b-5p helps in maintaining EpCAM+ cancer stem cells via HSC71/HSPA8 and augments malignant features in hepatocellular carcinoma Therapies targeting cancer stem cells (CSCs) in hepatocellular carcinoma (HCC) have limitations due to their similarities with normal stem cells (NSCs). Epithelial cell adhesion molecule (EpCAM) helps to enrich CSCs from EpCAM+AFP+HCC cases, but is also expressed on NSCs. Dr. Nirupma Trehanpati’s laboratory has discovered that miR-26b-5p target for both HSPA8 and EpCAM and was significantly reduced in Ep+CSCs. Importantly, it was found that miR-26b-5p imparts metastatic properties and helps in maintenance of EpCAM+CSCs via HSPA8. Thus, miR-26b-5p and HSPA8 could serve as molecular targets for selectively eliminating the Ep+CSC population in human HCCs (Khosla et al 2017 under review). ƒ The Multi-omics Facility (Dr Shvetank Sharma, Dr Jaswinder Maras, Prof. Shiv K Sarin) The multi-omics facility at ILBS involves a well integrated group employing genomics, metagenomics, proteomics and metabolomics, to understand the underlying processes in disease development and progression.  Genomics: The genomics lab at ILBS is directed towards understanding the basic layout of the liver genome and how it correlates with disease. The genomics unit has 140

recently determined a possible cause of lack of steroid response in severe alcoholic hepatitis patients utilizing next generation sequencing technologies (Sharma S et al Scientific Reports, 2017). The genomics group is now engaged in determination and characterization of gene communities that function in coherence and yield the effect at a system level. This reduction in granularity of ‘gene communities’ is being utilised to gain insight into the systems level functioning between patients who respond to steroids and those who don’t, by establishing ‘functional modules’. These modules can then be correlated with the specific clinical outcome, thus having a direct translational value.  Metagenomics: The study of microbial communities, and genes thereof, colonizing the human body has emerged as a powerful tool in understanding disease establishment and progression. Currently the lab is working on the alcohol induced metagenomic alterations in the gut and corresponding variations in functions of the hepatocytes. As a significant progress in liver disease treatment, ILBS has for the first time in the world run a pilot study on fecal microbiota transplantation (FMT) from a healthy stool donor in server alcoholic hepatitis patients, who could not be given steroids. The outcome of the study have been very promising.  Proteomics: Scientist at ILBS are utilising high resolution mass spectrometry coupled to advance labelling technologies for quantitative estimation of proteins in bio fluids and tissue samples of various liver diseases. Working on the urinary albumin in SAH patients we have clearly demonstrated that modification patterns of urinary albumin correlates with serum albumin and outcome in severe alcoholic hepatitis (S Das, Maras JS et.al 2017). We are also trying to utilize the D2O labelling in identification of time bound variations in the proteins synthesized in the human body at the time of setting in of the inflammation.  Metabolomics: The most complex of the omics technologies is investigation of the metabolome in cells and tissues. With liver as a highly metabolically active organ, it was natural to initiate investigations on the healthy and diseases human liver metabolome. With this study scientist at the ILBS intends to establish an Indian Liver Metabolome database (ILMD) to help clinicians and researchers to better understand the key players in the physiological processes. A major breakthrough has been characterization of urinary metabolites profile of serve alcoholic hepatitis patients. Using a metabolomics approach we identified L-acetyl carnitine as a prominent biomarker candidate for early(baseline) identification of non-responders to corticosteroid therapy in severe alcoholic hepatitis (abstract published as Maras et.al 2015, 2016) ƒ Iron Biology in Liver Disease (Dr. Jaswinder Maras and Prof. S.K.Sarin) Using a proteomics based approach we were the first to characterise iron overload associated mortality in plasma samples of ACLF patients (Maras.et al. Hepatology 2015). In this line we have now characterised the gene expression of severe alcoholic hepatitis patients with and without iron overload. This was further extended with the experiment on THP1 cells treated with Iron/Fenton reagent in presence and absence of 141

Deferiprone (Iron Chelator) (Maras et.al 2016). The main focus of our lab is to understand the pathophysiology of liver diseases and identify potential biomarker candidate which may be utilized for benefit of patients with liver disease. Scheme for Evaluating the metabolome in steroid responders vs non responders ƒ Bioartificial Liver Support Programme (Dr. Anupam Kumar and Prof. S.K.Sarin)  Development of an integrated hybrid cryogel based cellular bioreactor as a bioartificial liver support The liver injury is often massive and progressive, not all the time liver transplantation is possible, hence supportive strategies are needed to give time for native liver to regenerate. In this regard artificial and bio-artificial liver support system has potential application. In a collaborative initiative with IIT, Kanpur, we have developed a hybrid bioreactor with a compact design for improving the efficacy of the cryogel based bioreactor as a bioartificial liver support. This bioartificial support has shown efficacy 142

in supporting liver function both in-vitro and in-vivo condition of animal model of acute liver failure ( Published in Scientific Reports, 2017).  Decellularized liver matrix improve the potential of cryogel scaffolds for supporting hepatocyte growth and function Earlier studies have reported the possibility of repopulating decellularized matrices with hepatic cell lines or stem cells to improve liver regeneration. We now show versatility of decellularized liver matrix as a substrate coating of 3D cryogel scaffolds and decellularized liver matrices coated cryogels have significantly better ability to support growth and function of hepatocytes in comparision to non-coated scaffold bot in-vito and in-vivo (published in ACS Appl Mater Interfaces Journal. 2017). Design of integrated hybrid cryogel based bioreactor ƒ Exploring the Role of Angiogenesis in NASH Pathogenesis (Dr. Savneet Kaur and Prof. S.K.Sarin) Disruption of the liver vascular architecture, in particular angiogenesis, has been linked to progression of fibrosis, cirrhosis, and HCC in chronic liver diseases. We have now analyzed the angiogenic gene expression profile in patients with NAFLD (both steatosis and NASH) by microarray and RT-PCR studies. In the clinical analysis, the expression of some genes, namely, CCL2, VEGFR1, HO1 and RUNX1 showed significant correlations 143

with both fibrosis scores and NASH activity score of the patients. Runt-related transcription factor 1 (RUNX1), also known as acute myeloid leukemia 1 (AML1), is a powerful and pivotal regulator of haematopoiesis. Intriguingly, we observed no expression of RUNX1 in the hepatocytes and a very significant expression in the liver sinusoidal endothelial cells and the inflammatory cells in the biopsies. In the ongoing studies, we are studying the relationship between RUNX1 and angiogenesis in NASH by addressing several in vitro angiogenic studies and in vivo mice studies. ƒ Hepatostats (Dr Gayatri Ramakrishna, Dr Sukruti, Prof. S.K.Sarin) While actively the group is perusing in areas of liver regeneration to help the patients, it is equally challenging to understand the degenerative changes occurring in diseased liver for identification of therapeutic targets. In this regard the main areas of focus are microparticles as injury markers , growth arrest phenomenon of cellular senescence vs quiescence and nanoparticle delivery for elimination of cancer cells.  Microparticles in diagnostics of liver injury Circulating microparticles (MPs) are emerging as markers of immune cell stress. Work from our group has shown hematopoietic stem cell (CD34+) and Hepatocytes (ASGPR+) MPs were markedly higher in alcoholic hepatitis patients in steroid non- responder group and was also responsible for triggering sterile inflammation. These findings will certainly pave way for novel therapeutics and prognostic markers and revamp the management of sever alcoholic hepatitis (Sukruti et al, under review). In addition, we have now shown that hepatic MPs can act as reservoir of HBV infection and persistence and this novel work is suggestive of MPs as new therapeutic strategies to target HBV (Sukruti et al, under review).  Novel Hepatostats Earlier our group had identified SIRT2 as as a specific feature associated with stress induced premature senescence but not with either quiescence or cell death (Anwar et al, Cell Cycle, 2016). More recently we have identified gene expression signatures specific to senecnece and quiescence and and now report ARID5A as a quiescence associated marker (Anwar et al, Journal of Cell Physiology, 2017). Additionally we have shown that blocking NFAT2 pathway can effectively prevent malignant growth and viral protein can activate calcineurin-NFAT2 axis promoting growth and survival of epithelial cells (Ram et al, Exp Cell Res, 2017).  Targeted delivery of nanoparticles efficiently reduces hepatocellular carcinoma Here, we describe the synthesis and characterization of arginine α,β- dehydrophenylalanine (RΔF) nanoparticles for the selective delivery of tumor suppressive microRNA , miR-199a-3p which was achieved by conjugating lactobionic acid (LA) with ligand for the asialoglycoprotein receptor overexpressed in HCC . The nanoparticle showed an enhanced cellular uptake and an efficient delivery of miR- 199a-3p resulting in cellular apoptosis and downregulation of the specific target gene (mTOR) in vitro.amd NPs treated mice demonstrated >50% decline in tumor growth, 144

which also corresponded well with suppression of mTOR protein expression, tumor cell proliferation and increased survival rate (p<0.0.5). (Varshney et al , Hepatology, 2017). EXPERIMENTAL ANIMAL FACILITY (EAF) ILBS has an experimental animal facility to meet the requirements of the researchers for advanced Hepatobiliary research. The Experimental Animal Facility (EAF) at ILBS is registered with the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of Environment and Forests, Government of India vide renewed registration no. 1562/GO/Re/S/11/CPCSEA for conducting experiment on small animals such as rats and mice. The Animal Facility procures a variety of strains of rats and mice from the CPCSEA registered traders for the IAEC approved research projects for in-house researchers, which are used as animal models for understanding the human liver in health, diseases and cure. EAF also extends complete research and technical support to facilitate animal experimentation in the Institute. ILBS is committed to the highest standards of research and recognizes that laboratory animals must receive the best possible care, not only to obtain valid research data, but also to ensure the health and safety of animals, researchers, and animal caretakers. Animal facility ensures compliance of the statutory guidelines and all activities of the facility are carried out as per the standard operating procedures (SOPs). The Animal Facility maintains records of day-to-day activities as well as maintenance and experimentation as per the statutory requirement of CPCSEA. Qualified and trained veterinarians oversee all the animal health concerns, and provide all necessary veterinary and husbandry care to ensure that healthy animals are available for research. The staff of the animal facility ensures humane and appropriate animal care under the supervision of trained veterinarian. ƒ Training courses  Hands on Training for Laboratory Animal Science.  This training course imparts principles of three R’s, ethics, laws and guidelines on the regulation of scientific experiments on animals, general biology and reproduction of the laboratory animals, animal identification techniques, husbandry and care, sex differentiation, handling and restraint, and approved techniques for blood collection, injections, anaesthesia and monitoring, humane euthanasia, etc.  Total Number of PhD Student and Research fellow trained in 2017: 11 145

 Available facilities Animal house has facility to maintained immunodeficient, inbred and outbred strain of rodents. Following laboratory animals are maintained at the facility: Animal species: Mice: C57BL/6J (IB) and NOD/SCID (ID), nu/nu (ID) Rat: Sprague Dawley (OB)  Equipments: Individually Ventilated Caging System, Thermoregulated Surgical Platform, Isoflurane Inhalation Anaesthesia Machine, Animal Weighing Balance, Animal restrainers, Biosafety Hood, Refrigerator, Deep Freezer, Autoclave, CO2 chamber for Euthanasia.  IAEC Approved Projects in 2016-2017: 8  Animal Model of Liver Diseases at ILBS  Lieber-Decarli Liquid Diet-Alcoholic mice model  HFD/MCD induced NAFLD/NASH model  CCL4 induced liver fibrosis, cirrhosis and fibrosis related HCC  Thiacetamide (TAA) induced Liver fibrosis  Acetaminiphen (APAP) induced ALF animal Model  SQ Xenograft HCC model Lieber-Decarli Liquid Diet-Alcoholic mice model HFD/MCD induced NAFLD/NASH model 146

CCL4 induced liver brosis, cirrhosis and brosis related HCC Xenograft HCC model Staff Faculty Designation Dr. Shvetank Sharma Scientist in-Charge Dr. Anupma Kumari Veterinary Ocer) Mr. Karamjeet, Mr. Krishan Jr. Animal Technician Educational Activities Workshop/Meeting Date Flow cytometry program August 16th to August 18th Immunology Day April 29th Mammalian Cell Culture and Immunofluorescence March 1 to March 3 rd st Courses offered Courses Ph.D in Biomedical Sciences ( 5 seats) Short term and long training for MSc and MTech candidates for thesis Summer training programme Student Trainees Training INSA Student Name University Period Fellowship Jisha V Central University of Tamil Nadu May-July 2017 Yes Sathish S Bharathiyar University May-July 2017 Yes Uchit Bhaskar R.V. College of engineering May-July 2017 Yes Aakansha Kakran Amity University Dec 2016-Jun 2017 No Happy Chauhan Amity University Dec 2016-Jun 2017 No Anamika Banasthali Vidyapeeth Jan-Jun 2017 No Darpan Phagiwala JECRC University Jan-Jun 2017 No Abhishree Aggarwal Amity University May-July 2017 No Angel Bangalore University May-June 2017 No Waseem Ali Jamia Hamdard University June-July 2017 No Chandan Prasad Singh Sharda University June-July 2017 No 147

Department’s Achievements  The department was awarded the prestigious DST infrastructural grant to establish a facility for alternative methods for liver regeneration.  The department was awarded with the DST-FIST infrastructural grant (Level 1).  Early career grants awarded to Principal Investigators, Dr. Manish, Dr Sukruti, Dr Jasswinder for their liver-centric proposal.  Patent filed on micro RNA (miR-450b) as a non-invasive biomarker in pregnant women infected with Hepatitis E Virus (HEV) (Application No. 201711002807, patent authors are Dr. Nirupma Trehanpati, Prof. Shiv K Sarin, Dr. Sharda Patra.  Work on nanoparticles has been filed for patent. Dipeptide based Nanostructures for Targeted Delivery of miRNA for HCC treatment. Application No. 01611023040 (2016) Patent authors are Dr A Varshney, Dr J Panda, Dr A K. Singh, Dr.Rooge, Dr.S. Biswas, Prof. S K. Sarin, Prof. V S. Chauhan.  The department has received extramural fundings form national agencies for conducting focused research in areas of molecular medicine.  Students have presented their ongoing work in the prestigious national and international conferences like AASLD (3), EASL (2), ISGCON (4).  The students have bagged travel grants, bursaries, and best presentation awards in National and International meetings.  A total of 23 research articles have been published in the international peer reviewed journal with average impact factors of 4.5.  Few publications from the department have been cited extensively, more than 40 times.  Four students awarded PhD Degree in Biomedical Sciences. 148

 Award of DST grant for setting of Non-Transplant Therapeutic Strategies For Advanced Liver Diseases.  SERB Early career grants to Dr Sanal , Dr Sukruti, Dr Manish, Dr Jaswinder.  The department has received 7 major extramural funding form national agencies.  Work form the department has been published in peer reviewed international journals of high impacts.  The students have presented their ongoing work in the prestigious national and international conferences like AASLD, EASL, APASL and ISGCON.  The department also successfully conducted workshop on Flow cytometry applications and Mammalian Cell Culture and Immunofluorescence applications. Faculty and Students Achievements Name Achievements Dr. Sanal Fellowship to visit France Dr. Sukruti Fellowship to visit France EASL Travel Bursary Early Career Grant DST Dr. Jaswinder Singh Maras DST Grant Presidential Award ISGCON, India Dr. Manish DST Grant Ritu Khosla PhD Awarded Aditi Varshney PhD Awarded Ashish Kumar Vyas PhD Awarded Young Investigator with Registration bursary, EASL-2017 Young Investigator award third prize in oral presentation ISGCON 2017 Sukanta Das PhD Degree Awarded Bijoya Sen Poster in AASLD-2016, Boston, USA International DBT Travel Award th Invited for oral presentation in 7 Update on Hot Topics at AASLD-India 2017 Staff Faculty Designation Dr. N.K Ganguly Hony. Professor Dr. Vijay Kumar Professor Dr. Nirupma Trehanpati Additional Professor, Faculty In-charge Dr. Gayatri Ramakrishna Additional Professor Dr. Shvetank Sharna Associate Professor Dr. Anupam Kumar Assistant Professor 149

Dr. G.M. Sanal Assistant Professor Dr. Savneet Kaur Assistant Professor Dr. Jaswinder Maras DST-INSPIRE Faculty Dr. Anupama Kumari Veterinary Officer Adjunct Faculty Dr. Shyam Kotillil (Univ Maryland, USA), Dr. Devanshi Seth (Univ Syndney, Australia) Senior Research Fellows Dr. Sukruti Baweja and Dr Manish Choudhary Research Associates Dr. Tanvi Agarwal and Dr. Shabir Hussain PhD Students Ms. Ritu Khosla (Degree Completed), Ms. Aditi Varshney (Degree Completed), Ms. Sukanta Das(Degree Completed), Mr. Ashish Kumar Vyas (Degree Completed), Ms. Sadaf, Ms.Smriti Shubham, Mr. Dhananjay Mathur, , Ms. Bijoya Sen, Mr. Mojahidul Islam, Ms. Adil Bhat, Mr. Sachin Sharma, Ms. Sudristhi, Ms. Swati Arora, Ms. Savera, Ms. Sarita, Ms. Swati Thangriyal, Ms. Poornima, Mr. Jitender, Ms. Deepika, Ms. Hamda Siddiqui, Ms. Rashi Sehgal, Ms. Nisha Research Fellows Mr. Deepanshu Maheshwari, Ms. Nidhi Nautiyal, Ms. Rekha, Pooja Negi, and Varsha Dwivedi Technical Staff Mr. Dileep Singh, Ms. Surinder Kapoor, Mr. Arun Kumar , Ms. Mohini Sharma Academic and Research Achievements of Faculty Dr. Vijay Kumar Dr. Vijay Kumar obtained his PhD from the All India Institute of medical Sciences, New Delhi and received his post-doctorall training at Institute of Chemical Biology, Strasbourg, France. He has over thirty years of post- graduate teaching and research in Molecular Cell Biology and Molecular Virology. His work has focused on the oncogenic potential of HBx protein of Hepatitis B Virus (HBV) and has established its role in mitogenic signaling, DNA replication, cell cycle deregulation, ribosome biogenesis and ubiquitin-proteasomal system. Besides, his team has demonstrated the role of ‘Active DNA demethylation’ in DNA replication and transcription. His new research interests include miRNA based diagnostics, new antivirals for HBV and exosome-based drug delivery.  Total Number of publications 120  Total Number of publications in 2017 4  Total Number of Projects in 2017 1 Conference Attended RNA & Oligonucleotide Therapeutics, March 29-April 1, 2017 at Cold Spring Harbor laboratory, Cold Spring Harbor, USA. 150

Dr. Nirupma Trehanpati Dr. Nirupma Trehanpati is presently Additional Professor and Faculty-in- Charge of Department of Molecular and Cellular Medicine at ILBS. She obtained her PhD from Kurkeshtra University and did her postdoctoral studies in Medical College Wisconsin, USA. Her major interests are in Transplant and Hepatic immunology. She has more than 20 years of research experience. In the laboratory of Molecular and Cellular Immunology, major thrust is to understand the mechanism of the HBV vertical transmission, HBV reactivation, sepsis and liver failure. Our protocols are focused in understanding the regulatory parameters that influence the outcome of an immune response in HBV, HEV infection and alcohol induced injury. Group is basically involved in understanding the suppressive mechanisms of Regulatory T cells, Th17, Tfh, monocytes macrophages and Neutrophils.  Total Number of publications 62  Total Number of publications in 2017 14  Total Number of projects in 2017 3 Conference attended  Guest Lecture at Center for Research on Inflammation (CRI), INSERM.  University of Paris on 25th April, 2017.  Vice-Chairperson in IC-SCR at Datt Mediproducts Pvt Ltd (DMPL).  Faculty in WHO CC on Liver Diseases and Viral Hepatitis.  Course Co-coordinator of Flow-Cytometry workshops at ILBS.  Member Secretary of Institutional Animal Ethics committee (IAEC).  Member Secretary of Institutional Stem Cells Research committee (IC-SCR).  Applied one National Patent.  Recipient of Rashtriya Gaurav Award.  2 Abstracts were accepted in EASL 2017, Amsteradam, Netherlands.  2 Abstracts were accepted in AASLD 2017, USA. Dr. Gayatri Ramakrishna Dr. Gayatri graduated from Jawaharlal Nehru University and perused her postdoctoral work at National Cancer Institute, NIH, USA. Prior to joining ILBS she work as staff scientist at CDFD, Hyderabad. Her areas of research interests include understanding mechanisms which are important in stress response and key players of hepatostats i.e growth inhibition by senescence or quiescence.  Total Number of publications 47  Total Number of publications in 2017 5  Total Number of projects in 2017 1 151

Conference attended  Invited talk at the Indo-French meeting.  Invited talk on Sirtuins in DNA damage, Biochemistry TRS Meeting, Bangalore.  Poster Presentations in AASLD.  Appointed as reviewer for the Journals; Nature Reports, Scientic Reports, Nature Communication, Oncotarget, Hepatology International, Cell Molecular Life Sciences, Digestive Disease, World Journal of Gastroenterology and Hepatology. Dr. Shvetank Sharma Research focuses on elucidation of molecular pathways at cellular and systems level during disease progression using integrated analysis of genomic (total RNA, miRNA), proteomic, metabolomic and metagenomic data. An intense area of research in this regard is the gut microbial alterations during disease onset. Using the Next Generation Sequencing tools the group has analyzed the gut metagenome in patients of alcoholic liver disease. In the same set of patients, the PBMC transcriptome and plasma metabolome has also been analyzed. New tools are being developed for identifying transcriptome modules in blood and liver, that provide insight at a system level functioning of genes/cells during disease progression. The strongholds of the lab are employing mathematical modelling for analysis of the omics data and also that it is the only unit at ILBS to perform bioinformatic analysis using R and Python, besides other tools. Another area of investigation is genetic analysis of inheritance patterns of the potential candidate genes which when mutated results in genetic disorders which finally affect the liver functioning. The group has screened affected families to identify potentially susceptible individuals by mutation analysis screening.  Total Number of publications 22  Total Number of publications in 2017 8  Total Number of projects 2017 1 Dr. Anupam Kumar Dr Anupam kumar has graduated from All India Institute of Medical Science (AIIMS) , New Delhi in December 2006. He worked as postdoctoral fellow in UT MD Anderson Cancer Centre Huston Texas, USA from March 2007 to June 2011 before he Joined ILBS as Senior fellow research in September 2011 and become instructor in February 2012. Currently He is working as assistant professor since January 2013 and heading the laboratory of stem cell and Regenerative medicine. Major interest of his Group is to understand the mechanism of impairment of liver regeneration in Liver failure. Developing novel and effective regenerative medicine based therapeutic protocol to rescue liver regeneration and repair in liver failure, Liver tissue engineering and Development of bio-artificial Liver as an extra/intra corporal support for patient with acute and Acute on Chronic Liver failure. 152

 Total Number of publications 21  Total Number of publications in 2017 3  Total Number of projects 4 Dr. Sanal Madhusudana Girija Dr. Sanal is currently an Assistant Professor and the major interests of his laboratory are:  Making functional and transplantable ‘hepatocyte like cells’ from various cell types, especially induced pluripotent stem cells and embryonic stem cells.  Gene crafting using various genome editing tools, viral vectors, transposons etc.  Regeneration of the liver, especially the metabolomics and proteomics during the course of regeneration and termination. Studying liver regeneration in living donor liver transplantation (LDLT) will help us design better cell culture environments which would facilitate the generation and maintenance of hepatocytes which might engraft better upon transplantation, bridging the bench to bedside. We are also interested in the events that are involved in early development in mammals, how one cell becomes another, the gain and loss of cellular identity.  Total Number of publications 16  Total Number of project 1 Dr. Savneet Kaur Dr. Savneet Kaur obtained her PhD in Biochemistry from Institute of Genomics and Integrative Biology (IGIB), New Delhi and thereafter, she pursued DBT postdoctoral fellowship at Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCTIMST), Kerala. After her postdoctoral studies, she worked as a CSIR pool scientist the Department of Gastroenterology, GB Pant Hospital, New Delhi from. Later, she worked as an Assistant Professor in the School of Biotechnology, Gautam Buddha University, Greater Noida from 2010-2017. She has specialization in clinical immunology, stem cell biology and angiogenesis. Since last few years, her efforts are focused to investigate the role of EPC-mediated angiogenesis in liver physiology and pathophysiology. vascularization strategies for effective liver regeneration.  Total Number of publications 26  Total Number of publications in 2017 2  Total Number of ongoing project 1 153

Dr. Jaswinder Singh Maras Dr. Maras is currently a DST Inspire faculty. He received his PhD in Medical Sciences from Delhi University . His area of interests include high-throughput technologies in areas of proteomics and metabolomics. The main focus of his laboratory is to understand the pathophysiology of liver diseases and identify potential biomarker candidate which may be utilized for benefit of patients with liver disease.  Total Number of publications 11  Total Number of publications in 2017 4  Total Number of ongoing projects 2 Awards First prize in the presidential poster at ISGCON-2017, Bhubaneshwar 2017for the work on Iron promotes ADAM-17 mediated sCD163: TNF-α liberation and modulates inflammation in severe alcoholic hepatitis”. Dr. Sukriti Dr. Sukruti received her PhD in medical sciences from Delhi University with specialization in clinical immunology, vascular biology and molecular biology. She has three years of postdoctoral training at University of Illinois at Chicago (UIC), USA. She was a recipient of highly meritorious American Heart Association (AHA) postdoctoral award. She has also specialized in studying microvesicles (MVs), its putative role in intercellular communication and in mediating transfer of genetic material. Presently she is deputed at Centre for research on Inflammation, INSERM, Xavier Bichat School of Medicine (organisation MOU with ILBS) to work on Immuno-modulatory pathways during liver diseases and also to investigate immune modulation by cell specific endosomes.  Total Number of publications 20  Total Number of publications in 2017 2  Total Number of project 1 Awards Awarded with Young investigator full bursary award at European association for study of liver meeting at Amsterdam, Netherlands 2017 to present poster at the Annual meeting. 154

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Clinical Research 156

Clinical Research Clinical Research The clinical research facility at ILBS, conducts multi-centric, global, complex clinical trials of new therapies, molecules to meet the unmet need of medical sciences. Such complex clinical research and epidemiological studies are being conducted by the ILBS clinical faculty and dedicated staff specialized in conducting clinical trials as per the international standards, which are highly specialized in the area of Liver and Biliary sciences and have a vast experience in medical research with major publications. Experienced research staff shepherd studies through a required safety approval by the ILBS’s highly competent Institutional Ethics Committee and Drug Controller General of India, counsel and recruit constantly monitoring and laying of the patients throughout the study, and ensure compliance with privacy regulations and Good Clinical Practices. ILBS carries out Clinical Trials in various areas like drug trials, in-house, national & International projects. The Clinical Research Department of ILBS is run by a team of active workers who coordinate the various activities and maintain quality in research as per ICH-GCP Guidelines and other regulatory authorities (DCGI, FDA, EMEA, and the like). Clinical research determines the safety and effectiveness of medications, devices, diagnostic products and treatment regimens intended for human use. These may be used for prevention, treatment, diagnosis or for relieving symptoms of a disease. Clinical trials involving new drugs are commonly classified into four phases. Each phase of the drug approval process is treated as a separate clinical trial. The drug-development process will normally proceed through all four phases over many years. If the drug successfully passes through Phases I, II, and III, it will usually be approved by the national regulatory authority for use in the general population. Phase IV are ‘post- approval’ studies. 157

The Clinical Research at ILBS provides the bridge between medical research and patient care. Patients, however, may derive benefit from clinical trials by gaining early access to new treatments. Most, however, have altruistic motives and participate in order to further the understanding or treatment of particular diseases. Patients who join clinical trials receive access to new treatments and the dedicated attention from physicians, research nurses and coordinators. Patients are getting these new treatments and investigations free of cost, which otherwise cost very high. In short span the facility of clinical research has established itself and attained vital position in the Institute’s objective and mission of being pioneer institution in research. Currently there are many clinical trials ongoing in different stages. In almost every clinical trial we have enrolled highest numbers of patients globally at ILBS which has enhanced the image of the Institution and set standards of clinical research among one of the top at the International level. We are being looked by multinational companies and CROs as major recruiting site with state of art facilities and dedicated staff. With clinical research expanding its arms at ILBS, there is an immediate effect seen on raising the funds for the Institution. We have been getting fund from International and National agencies. Most of faculty of the department is member Ethical, SRB and IRB. The department also helps in smooth conduct of various research trials (internal as well as external). The clinical research department has initiated new courses like: 1. Post graduate Certificate course ( Epidemiology) 2. Post graduate Certificate course ( Clinical Research) 3. PhD program (Epidemiology of Viral Hepatitis and Liver Diseases) And also developed academic curriculum for the PGCC (Epidemiology), PGCC (Clinical Research) and PhD program (Epidemiology). Another course in certificate course in Biostatistics is on the way and hopefully it will be materialized in the coming year. 158

Courses Offered Name Candidate Admitted 1. Post graduate Certificate course ( Epidemiology) 2. Post graduate Certificate course ( Clinical Research) Dr. Ankit 3. PhD program (Epidemiology of Viral Hepatitis and Liver Diseases) Short Term Training Training Participants Hands on Practices on medical data” Thirty eight participants, all faculty members from various medical 16th August to 19th 2017during the period college attended the workshop and was highly appreciated Department’s Achievements  Department conducts regular statistical and research methodology classes for students from within and also attracts outside the Institute.  Successfully conducted the hands on training in statistical methodology which was attended by candidates from all over India and this course was highly appreciated by the participants.  The Department has actively participated in coordination of various clinical trials as listed below. Phase Indication #of studies handled Sponsor III Chronic HBV (HBeAg-Negative) 01 Gilead III Chronic HBV (HBeAg-Positive) 02 Gilead III b HCV (Chronic Genotype 1 or 3) 01 Gilead observational Chronic HBV & HCV 01 Gilead III Liver Cirrhosis- HCV related 01 NATCO III Hepatocellular Carcinoma 02 BMS II Chronic HCV 01 Pfizer III Chronic Liver Disease 01 Exalenz III Acute on Chronic Liver Failure 01 Exalenz IV Chronic HCV 01 Gilead III NASH 02 Gilead Staff Faculty Designation Dr. Ankit Bhardwaj Jr. Clinical Trial Coordinator Dr. Priyanka Jain Statistician Dr. Guresh Kumar Instructor (Research) Technical Staff Ms. Mohini Sharma 159

Academic and Research Achievements of Faculty Dr. Guresh Kumar Dr. Guresh Kumar is working as an Instructor (Research). He has done Post Graduation and M.Phil from Punjab University, Chandigarh. He is also a Doctor of Philosophy (Ph.D.) in Statistics from UOR Jaipur. He has vast experience of more than 30 years in the prestigious All India Institute of Medical Sciences (AIIMS), New Delhi in the fields of Biostatistics. He is involved in teaching and research activities of DM, M.Ch. and Ph. D. scholars in the Institute. He is facilitating the faculty, residents and PhD scholar from protocol development to final data analysis during their research projects and thesis work. He is an active member of institutional ‘Data Safety and Monitoring Board (DSMB)’ and WHO CC on viral hepatitis and liver diseases. He is also a member of Institute Research Board and SRB committee. The achievements during the period ending with 2017 are:  Invited as a resource person twice in a workshop conducted by ICPO (ICMR).  Reviewer for three renowned national/International journals indexed in Medline.  Active member of three prestigious National associations (Indian Society of Medical Statistics (ISMS), Indian Association of Medical Informatics (IAMI), and Indian Association of Preventive and Social Medicine (IAPSM)).  He attended the Indian Society on Medical Statistics (ISMS) conference held at SGPGI Lucknow.  Conducted a 04 days’ workshop from 16th August to 19th 2017 entitled “Hands on Practices on medical data” during the period. Thirty eight participants, all faculty members from various medical college attended the workshop and was highly appreciated.  Total Number of publications 114  Total Number of publications in 2017 15 Dr. Ankit Bhardwaj Dr. Ankit Bhardwaj is presently working as a Jr Clinical Trial Coordinator in Department of Clinical Research at ILBS. He has done Post Graduate Diploma in Clinical Research from IIPH, PHFI. He is managing all the issues related with clinical trials (both internal as well external undergoing projects). He is also helping the Ethitical committee member for its smooth conduction. He is also helping the DM/ PDCC students in the passing of their research protocol. He is mainly responsible for smooth conduction of clinical trials undergoing in the Institute, starting from data collection, consent, drug distribution and finally the result after updating the data. He is also acting as liaison officer between Institute and the various external pharmaceutical companies. He has vast experience of handing Internal, External & Sponsor audits. 160

 Total Number of publications 10  Total Number of publications in 2017 06 Conferences attended  Poster presentation in an international conference (AASLD 2016) Boston, USA.  Poster presentation in national conference (ISG 2016) New Delhi, India. Dr. Priyanka Jain Dr Priyanka Jain is presently working as a Statistician in the department of clinical research at ILBS. She has done M.Sc (Distinction), M.Phil (Honours) and Doctor of Philosophy (Ph.D.) in Statistics from Dr B. R. A. University Agra. She has three years experiences as Assistant Professor in Degree College and four years and seven months experiences as a Statistician in research.  Total Number of publications 12  Total Number of publications in 2017 6 Conferences attended  Poster presentation in an international conference (AASLD 2017) in Washington, 20-24 October,2017.  Poster presentation in an international conference (AASLD STC 2017) in Chicago, 15-16 September, 2017.  He is also helping the Ethitical committee member for its smooth conduction.  Presently she has a responsibility of:  As a Project coordinator cum Statistician at AARC (APASL ACLF Research consortium), a largest patient database with multicentric-multinational project. She is actively involved in managing online database as well as helping investigators in their compiling and analyzing the data.  Actively involved in collecting relevant information, developing database and data analysis for the protocols of AKI (Acute Kidney Injury) in Intensive care Unit (ICU).  She is actively involved in biostatistics workshop and conferences held in ILBS for their smooth conduction.  Help faculty, Senior Residents, DM and PDCC students in extracting, compiling and analyzing the data and provide results into graphs and tables as per requirement.  Helping in analysis of quality control (NABH) as per requirement in the department of medical operation.  Handling clinical data of ILBS database and provide relevant information, prepare graph as per requirement of ILBS. 161

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Academics 163

Academics 164

Academics Institute of Liver and Biliary Sciences (ILBS) is a premier medical institute established by the Government of the National Capital Territory (NCT) of Delhi as an Autonomous Super- speciality Institute, under the Societies Registration Act - 1860, New Delhi. Conceptually, this has been planned and executed as an innovative, medical institution not only for patient-care but also to undertake advance research and educational teaching programmes in the field of Liver and Biliary diseases. Besides imparting quality care patient service, a major vision of the Institute is to provide world-class training, teaching and performing cutting-edge scientific research in a focused niche area of liver and biliary diseases. ILBS has been granted a deemed-to-be-University status was granted by the University Grants Commission (UGC) under Section 3 of the UGC Act, 1956 under the de-novo category through the Ministry of Human Resource Development, Government of India. This deemed to be university is a ‘de-novo institute with a promise for excellence’ in Hepatobiliary Medicine, Surgery and Research and is a unique institute that is dedicated to patient management, teaching and research. The Institute of Liver and Biliary Sciences was graded ‘A’ in Year 2017 by the National Assessment and Accreditation Council (NAAC). The institute and its vision were conceived by eminent academicians and visionaries to provide world-class training and teaching, including excellent patient care and cutting- edge research for curing liver and biliary diseases. All activities of the university have been directed towards this vision . ILBS offers training programs for super-specialties related to Hepato-Biliary and Pancreatic Sciences through Post-Doctoral 3-year degree courses: DM (Hepatology), DM (Paediatric). Hepatology), DM (Organ Transplant Anaesthesia and Critical Care) and M.Ch. in Hepato- Pancreato-Biliary Surgery. These degree programmes are duly approved by the Medical Council of India. The Institute offers PhD programmes in Biomedical Sciences and Clinical 165

Nutrition and Epidemiology. Apart from these degree courses, ILBS also has several Post- Doctoral Certificate Courses (PDCC) in various specialities viz Liver Transplant Anesthesia, HPB Interventional Radiology, Pediatric Hepatology, Hepatopathology, Nephrology, Hepato-Virology, Critical Care Medicine (with specialization in Liver Intensive Care), HPB/ GI Oncology, Abdominal Imaging, Blood Banking and Immunohaematology, Apheresis, Clinical Microbiology, Clinical Nutrition, Epidemiology and also Certificate Courses in Nephrology. ILBS also provides observership and short-term training programs in various specialities. The aim of these programs is to provide targeted training and mentorship designed to impart important skills. The students seeking admission in various courses are selected through open and transparent examination system. The Institute focuses on high quality patient care, training of international standards and health research of social relevance and has been successfully running the enumerated Doctoral and other research Degree programs through open all India competitive Examinations. World Gastroenterology organization (WGO) and Asia Pacific Association of Study of Liver Disease (APASL) have decided to use our campus as well as faculty for the short training programs in the field of hepatology. Our Institute regularly runs APASL-supported School of Hepatology program in which many foreign fellows/ junior consultants are offered three months training on different aspects of hepatology like portal hemodynamics, endoscopic ultrasound, liver support systems and pediatric liver diseases. In addition the institute has started APASL supported international program of College of Hepatology in which foreign delegates are trained. Examination at ILBS The admission to DM/M.Ch. courses in Year 2017 has been made through National Eligibility cum Entrance Test Super Specialty Examination (NEET SS) organised by the National Board of Examination. The admission to PDCC/Ph.D. and Certificate courses at ILBS is done through an open and transparent examination system on an All India basis. The admission notification is issued in the month of March and September every year inviting applications from eligible candidates to appear for the entrance Examination scheduled on the last Sunday of May and the second Sunday of December for the academic session July and January respectively. The admission notification is issued in the press and also on the Institute website. The applications are invited from eligible candidates through an online system and after scrutiny, for eligibility for various courses of ILBS, candidates are called for the entrance examination. The entrance examination consists of two parts viz. Theory Examination and Department Assessment Examination. The Result of the Theory Examination is declared on the same day in the evening (Day 1), and displayed on the website, and successful candidates are called for the Department Assessment examination on very next day (Day 2). The final result based on the combined performance of the Theory and the Department Assessment examination declared in the evening (Day 2), and also displayed on the Institute’s website. In the interest of fairness and transparency, all results are declared and displayed same day on the website. The Examination committee, consisting of Senior faculty members of ILBS, ensures validity and reliability of the Examination so as to incorporate necessary checks at different stages to hold the examination effectively. 166

Hostel Accommodation The Institute of Liver and Biliary Sciences, New Delhi is a fully residential complex. All residents have to compulsorily reside in the campus during their course of study. The older Adhayan Hostel at ILBS was established to provide accommodation facilities for the Residents’ and Research Scholars enrolled in various courses. The recently acquired New Hostel accommodation consists of a 1-room apartment including kitchenette and living area to provide comfortable stay to the residents and their families. The close proximity, 24 hour internet facility, and compact unit provided helps the students to focus on the training. The main objective of the hostel is to provide a comfortable, homely stay, while allowing intellectual stimulation, cross-cultural interaction and freedom with responsibility to the residents. This Hostel is aesthetically designed in a wide space. The common room and open green area attached to it provides moments of leisure and promotes interaction amongst the students. The entire area is Wi-Fi enabled for the convenience of the students. There is a designated well maintained visiting area for visitors. The warden and the assistant warden look after the internal administration and day-to-day discipline. Hostel Officials Dr. Senthil Kumar, Addl. Professor (HPB Surgery) Senior Faculty Incharge Dr. Ankur Jindal, Asstt. Professor (Hepatology) Junior Faculty Incharge E-library The E-Library at ILBS supports the academic as well as the research needs of students and faculty of the University round the clock with its 131 online journals. The E-Library can be accessed by Residents’ and Faculty from all corners of the Hospital and Hostel . Its collection comprises hybrid resources (Print and Electronic) of books, theses and journals. It houses 536 books, 49 theses, 330 bound volumes of journals. It also subscribes 131 full text scholarly current e-Journals to keep users updated with trends in their field. ILBS library has received an award for highest usage of the Wiley journals in 2017 within ERMED consortium (Tier II) by Wiley on 20th December 2017. 167

Academic Programs Degree Courses S. No. Name of Course Duration Seats 1* DM Hepatology 3 Years 4 Seats 2* DM Organ Transplant Anaesthesia and Critical Care 3 Years 1 Seat 3* DM Paediatric Hepatology 3 Years 1 Seat 4* M. Ch Hepato-Pancreato-Biliary Surgery 3 Years 3 Seats 5* DNB (Nephrology) 3 Years 2 seats 6 MD-PhD 5 Years 4 Seats 7 Ph.D. in Biomedical Sciences 5 Years 10 Seats 8 PDCC in Liver Transplant Anesthesia 1 Year 3 Seats 9 PDCC in HPB Interventional Radiology 1 Year 3 Seats 10 PDCC in Pediatric Hepatology 1 Year 3* Seats 11 PDCC in Hepatopathology 1 Year 3* Seats 12 PDCC in Nephrology 1 Year 3* Seats 13 PDCC in Hepato-Virology 1 Year 2 Seats PDCC in Critical Care Medicine 14 1 Year 3 Seats (with specialization in Liver Intensive care) 15 PDCC in HPB / GI Oncology 1 Year 2 Seats 16 PDCC in Abdominal Imaging 1 Year 3 Seats 17 PDCC in Blood Banking and Immunohaematology 1 Year 2 Seats 18 PDCC in Advanced Diagnostic and Clinical Microbiology 1 Year 1 seat 19 PDCC in Apheresis Technology 1 Year 2 seats 20 Ph.D. in Clinical Nutrition 5 Years 2 Seats 21 PGCC in Clinical Nutrition 1 Year 3 Seats 22 Ph.D. in Epidemiology of Viral Hepatitis and Liver Diseases 5 Years 1 Seat 168

23 PGCC in Epidemiology of Viral Hepatitis and Liver Diseases 1 Year 2 Seats 24 PGCC in Clinical Research in Liver Diseases 1 Year 2 Seats 25 Certificate Course in Nephrology 6 Months 2* Seats 26 Critical Care Certification Course 6 months 2 seats *Admission to the courses mentioned at S.No. 1,2,3,4 and 5 is through NEET (SS) w.e.f Academic Year 2017. The admission to PDCC courses are offered twice in a Year. Degree Courses S. No. Name of Course Duration Seats 1 Short Term Training in HVPG and TJLB 3 months 2 seats every 3 months Short Term Training in Department of Molecular and 2 1-6 months Variable Cellular Medicine 3 Short Term Training in Clinical Virology 3 months 2 seats every 3 months 4 Short Term Training in Hepatopathology 6 months 2 seats every 6 months Short Term Training in Hematology (for technical 5 3 months 2 seats every 3 months grade) 6 Short Term Training in Hematology (for MD/DCH) 3 months 1 seat every 3 months Short Term Training in Biochemistry (Automation in 7 3 months 2 seats every 3 months Diagnostics) Short Term Training in Biochemistry (Immunological 8 3 months 2 seats every 3 months Techniques) Short Term Course in Hepato-Pancreato-Biliary 9 3 months 2 seats every 3 months Radiology 10 Short Term Training in Transient Elastography 2 weeks 2 seats / course 11 Short Term Training in Endoscopic Ultrasound 3 months 2 seats every 3 months Student Enrolled in Year 2017 July ’2017 DNB, PDCC and Ph.D Batch 1 Dr. Dibya jyoti Kalita DNB (Nephrology) 08.05.2017 2 Dr. Anuradha DNB (Nephrology) 17.05.2017 3 Dr. Harshita Omprakash Singh PDCC- Liver Transplant Anesthesia 01.07.2017 4 Dr. Udit Dhingra PDCC- Liver Transplant Anesthesia 07.07.2017 5 Dr. Juhi Chawla PDCC- Hepatopathology 03.07.2017 6 Dr. Shaloo PDCC- Abdominal Imaging 01.07.2017 7 Dr. Subha Ashok PDCC- Abdominal Imaging 01.07.2017 8 Dr. Sumit Singhania PDCC- Abdominal Imaging 01.07.2017 9 Dr. Brinda Kakkar PDCC- Blood Banking and mmunohaematology 01.07.2017 169

10 Dr. Ayushi Tayal PDCC- Hepato Virology 01.07.2017 11 Dr. Sowmya B. U. PDCC- Hepatopathology 01.07.2017 12 Dr. Tanvi M. Meshram PDCC- Liver Transplant Anesthesia 01.07.2017 13 Dr. Aniket Mondal PDCC-Interventional Radiology 01.07.2017 14 Dr. Lalit Garg PDCC-Interventional Radiology 01.07.2017 15 Dr. Swati Das PDCC-Interventional Radiology 01.07.2017 PDCC-Advanced Diagnostic and Clinical 16 Dr. Pradheer Gupta 14.07.2017 Microbiology 17 Ms. Swati Thangariyal PhD-Biomedical Sciences 01.07.2017 18 Ms. Harshita Tripathi PhD-Clinical Nutrition 01.07.2017 19 Ms. Purnima Tyagi PhD-Biomedical Sciences 08.08.2017 September’2017 DM/M.Ch. Batch 1 Dr. Sushrut Singh DM Hepatology 21.08.2017 2 Dr. Satyam Sinha DM Hepatology 13-09-2017 3 Dr. Vinay Kumar B.R. DM Hepatology 14-09-2017 4 Dr. Rakesh Kr. Jagdish DM Hepatology 20-10-2017 5 Dr. Sahil Gupta MCh. HPB Surgery 13-09-2017 6 Dr. Vivek R MCh. HPB Surgery 13-09-2017 7 Dr. Venkatesh. B.S. MCh. HPB Surgery 13-09-2017 8 Dr. Neha Tandon DM Organ Transplant Anaestheisa 13-09-2017 9 Dr. Priti Vijay DM Pediatric Hepatology 21-10-2017 January’2018 PDCC/Ph.D. Batch 1 Dr. Nimje Ganesh Ramaji PDCC- Liver Transplant Anesthesia 01.01.2018 2 Dr. Lakshmi Kumar Chalamarla PDCC-HPB Interventional Radiology 01.01.2018 3 Dr. Krishna Bhardwaj PDCC-HPB Interventional Radiology 01.01.2018 4 Dr. Arpit Taunk PDCC-HPB Interventional Radiology 01.01.2018 5 Dr. Neha A Sharma PDCC- Hepato Virology 01.01.2018 6 Dr. R Krithiga PDCC- Hepato Virology 01.01.2018 7 Dr. Gaurav Khanna PDCC- Hepatopathology 01.01.2018 8 Dr. Riti Yadav PDCC- Hepatopathology 01.01.2018 9 Dr. Trupti Laxman Lokhande PDCC-Apheresis Technology 01.01.2018 10 Dr. Dnyaneshwar Shridharrao Patale PDCC-Apheresis Technology 01.01.2018 11 Dr. Ansuman Sahu PDCC- Blood Banking and Immunohaematology 01.01.2018 12 Dr. Smita Chouhan PDCC- Blood Banking and Immunohaematology 01.01.2018 PDCC-Advanced Diagnostic and Clinical 13 Dr. Nitya Batra 01.01.2018 Microbiology PDCC in Critical Care Medicine (With specialization 14 Dr. Sudhir Kumar Bisherwal 01.01.2018 in Liver Intensive Care) 170

PDCC in Critical Care Medicine (With specialization 15 Dr. Shilpa Tiwari 01.01.2018 in Liver Intensive Care) PDCC in Critical Care Medicine (With specialization 16 Dr. Shailesh Kumar Sahu 01.01.2018 in Liver Intensive Care) Academic Calendar The Institute of Liver and Biliary Sciences, Deemed-to-be-University academic calendar provides important planning information for students and faculty. The calendar includes semester start dates, deadlines for Thesis, exam dates, and much more time-sensitive information. The Academic Calendar for Year 2018 is mentioned below: Academic Calendar for 2018-19 Date of the Examination Name of the Course Name of the Activities 1st January 2018 PDCC / PhD Induction of new batch admitted in January 2018 Thesis submission of DM/M.Ch. batch admitted in Year 31st January 2018 DM/M.Ch. 2015 Project Proposal submission of batch admitted in January 28th February 2018 PDCC 2018 26th May 2018 PDCC/PhD and CC Entrance Written Examination 27th May 2018 PDCC/PhD and CC Entrance Departmental assessment 31st May 2018 PDCC Project Submission of batch admitted in July 2017 4th , 5th, 6th and 7th DM/MCh Departmental Prefinal June’2018 11th, 13th and 15th June PDCC Exit Theory Exam PDCC batch admitted in Year July 2017 2018 19th June 2018 PDCC Exit Practical Examination DM/MCh/PDCC /PhD 2nd July 2018 Induction of new batch admitted in July 2018 and CC 07th ,09th, 11th and 13th DM /MCh Exit Theory Examination of the batch admitted in July 2015 July 2018 17th and 18th July 2018 DM /MCh. Exit Practical Examination Submission of Project Proposal of batch admitted in July 31st August 2018 PDCC / CC 2018 Project Submission for PDCC batch admitted in January 30th October 2018 PDCC 2018 11th , 12th , 13th and 14th Nov 2018 DM and MCh Departmental Midterm/Semester Examination 15th , 16th and 17th November 2018 PDCC Departmental Pre-final of batch admitted in January 2018 171

3rd , 5th and 7th December 2018 PDCC/CC Theory Examination batch admitted in January 2018 9th December 2018 PDCC/CC Practical Examination 16th December 2018 PDCC/CC Entrance Written Examination 17th December 2018 PDCC/CC Entrance Departmental assessment 29th December 2018 DM/M.Ch. Submission of Thesis protocols batch admitted 01/07/2018 31st December 2018 DM/M.Ch. Thesis submission of batch admitted in July 2016 Academic Excellence Residents of various courses at ILBS attended International Conferences in Year 2017 funded by ILBS / Government Funding Agencies i.e. DST, ICMR, CSIR to enhance their knowledge and exposure. SN Name of Resident Course Detail Conference Attended 1 Dr. Arti DM Pediatric Hepatology APASL 2017, Shanghai, China 2 Dr. Arti DM Pediatric Hepatology AASLD 2017, Washington DC 3 Dr. Babu Lal Meena PDCC Pediatric Hepatology APASL 2017, Shanghai, China 4 Dr. Sheetal Ganju PDCC Pediatric Hepatology MCLD 2017, Global Hospital 5 Dr. Ayushi Agarwal PDCC Hepatopathology APASL 2017, Shanghai, China The International Liver ongress 2017, 6 Dr. Varsha Shashtry PhD Clinical Nutrition Amsterdam, Netherlands 2017 Joint Congress of ILTS, ELITA 7 Dr. Rommel S MCh HPB Surgery and LICAGE, Prague, Czech Republic 2017 Joint Congress of ILTS, ELITA 8 Dr. Nihar R. Mohapatra MCh HPB Surgery and LICAGE, Prague, Czech Republic 2017 Joint Congress of ILTS, ELITA 9 Dr. Ankur Vagadiya MCh HPB Surgery and LICAGE, Prague, Czech Republic Dr. Sujata Lall National Virology Conference, 10 PDCC Hepato Virology CMC Vellore, India Dr. Sujata Lall 11 PDCC Hepato Virology CIDSCON 2017, Nagpur, India Dr. Tanvi M Meshram PDCC Liver Transplant AAGBI Annual Conference 2017, 12 Anaesthesia Liverpool, England Dr. Brinda Kakkar PDCC Blood Banking and TRANSMEDCON 2017, Lucknow, 13 Immunohaematology India Dr. Pradheer Gupta PDCC Advanced Diagnostic MICROCON 2017, RIMS Ranchi, 14 and Clinical Microbiology India 15 Dr. Sahil Gupta MCh HPB Surgery ESMO Asia 2017, Singapore Dr. Ayushi Tayal MICROCON 2017, RIMS Ranchi, 16 PDCC Hepato Virology India 172

ISGCON 2017, Bhubaneswar, 17 Ms. Harshita Tripathi PhD Clinical Nutrition India PhD Molecular and Cellular 18 Mr. M Islam AASLD, 2017, USA Medicine PhD Molecular and Cellular 19 Ms. Bijoya Sen AASLD India Medicine PhD PhD Molecular and Cellular 20 Mr. Ashish Vyas ISGCON, 2017, Orissa Medicine PhD PhD Molecular and Cellular 21 Mr. Sukanat Das ISGCON, 2017, Orissa Medicine Trainees at ILBS Residents from various Institute undergone training/observership in Year 2017 to attain expertise in their speciality. Name of S N. Course Detail Country Duration Resident Doctoral Resarch Fellow, New Zealand Liver New 18-12-2017 to 1 Dr. Debi Prasad Transplant Unit Zealand 19-01-2018 Sr. Resident, Dept. of Radiodiagnosis, PGIMS 01-12-2017 to 2 Dr. Nitin Yadav India Rohtak 15-12-2017 Sr. Resident, DM-Hepatology, PGIMER 3 Dr. Jamil Shah India Chandigarh Dr. Roshan Sr. Resident, DM-Hepatology, PGIMER 15-10-2017 to 4 India Agarwala Chandigarh 11-11-2017 Dr. Katrine Holtz Research Assistant, Gastroenterology and 15-10-2017 to 5 Denmark Thorhauge Hepatology, University of Southern Denmark 11-11-2017 12-09-2017 to 6 Dr. Karen Manukyan GI Endoscopist, Armenia Armenia 11-10-2017 Dr. Kamala Retnam Consultant Radiologist, JJ Hospital, Anna Nagar, 08-09-2017 to 7 India Mayilvaganan Madurai 08-10-2017 Sr. Resident, DM-Hepatology, PGIMER 01-09-2017 to 8 Dr. Siddharth Shukla India Chandigarh 15-09-2017 Sr. Resident, DM-Hepatology, PGIMER 01-09-2017 to 9 Dr. Balaji India Chandigarh 15-09-2017 Addl Prof., Anesthesiology and Critical Care, 24-07-2017 to 10 Dr. Suman Lata India JIPMER, Puducherry 22-08-2017 Sr. Resident Devision of Pediatric 11 Dr. Subhamoy Das Gastroenterology, Dept of Gastroenterology, India 01-07-2017 to 15-07-2017 PGIMER Chandigarh 173

Sr. Resident Devision of Pediatric 12 Dr. Srikanth KP Gastroenterology, Dept of Gastroenterology, India 16-06-2017 to 30-06-2017 PGIMER Chandigarh Sr. Resident, DNB Gastroenterology, Department 31-05-2017 to 13 Dr.Rohan Badave of Gastroenterology, KMC hospital, Dr.BR India Ambedkat Circle, Mangaluru 30-06-2017 Prof. and Head, Gastroenterology, KMC Hospital, 31-05-2017 to 14 Dr. BV Tantry India Dr. BR Ambedkar Circle, Mangaluru, 575001 30-06-2017 Sr. Resident, DM Gastroenterology, PGIMER 03-05-2017 to 15 Dr. Rizwan Ahmed India Chandigarh 17-05-2017 Dr. Bhim Ahmed Sr. Resident, DM Gastroenterology, PGIMER 03-05-2017 to 17- 16 India Kettagiri Chandigarh 05-2017 03-05-2017 to 17- 17 Dr. Saurabh Jaiswal Sr. Resident, DM Hepatology, CMC Vellore India 05-2017 Sr. Resident, DM Gastroenterology, PGIMER 01-05-2017 to 14- 18 Dr. Atul Rana India Chandigarh 05-2017 Sr. Resident, DM Hepatology, Madras Medical 16-04-2017 to 30- 19 Dr. M Jaiganesh India College, Chennai 04-2017 Department of Hepatology, School of Digestive 16-03-2017 to 30- 20 Dr. Jiban Basak India and Liver Diseases, 03-2017 Sr. Resident, DM Hepatology, Meenakshi Medical 09-03-2017 to 07- 21 Dr. Rajan Mittal India College, 04-2017 Dr. S. Sr. Resident, DM Hepatology, Madras Medical 01-03-2017 to 15- 22 India Kumaragurubaran College, Chennai 03-2017 Sr. Resident, DM Hepatology, Meenakshi Medical 01-03-2017 to 30- 23 Dr. Padmanabham India College, Kanchipuram 03-2017 Consultant, Department of Gastroenterology, 27-01-2017 to 17- 24 Dr. Abid Shoukat India Bansal Hospital, Bhopal, Madhya Pradesh 02-2017 Short Courses S N. Name of Course Duration Seats 1 Short Term Training in HVPG and TJLB 3 months 2 seats every 3 months 2 Short Term Training in Research Department 1-6 months Variable 3 Short Term Training in Clinical Virology 3 months 2 seats every 3 months 4 Short Term Training in Hepatopathology 6 months 2 seats every 6 months 5 Short Term Training in Hematology (for technical grade) 3 months 2 seats every 3 months 6 Short Term Training in Hematology (for MD/DCH) 3 months 1 seat every 3 months Short Term Training in Biochemistry (Automation in 7 3 months 2 seats every 3 months Diagnostics) 174

Short Term Training in Biochemistry (Immunological 8 3 months 2 seats every 3 months Techniques) 9 Short Term Course in Hepato-Pancreato- Biliary Radiology 3 months 2 seats every 3 months 10 Short Term Training in Transient Elastography 2 weeks 2 seats / course 11 Short Term Training in Endoscopic Ultrasound 3 months 2 seats every 3 months Successful Candidates in Year 2017 S N. Name of Student Designation 1 Dr. Vinod Arora DM Hepatology 2 Dr. Vikash Prakash DM Hepatology 3 Dr. Sumeet Kainth DM Hepatology 4 Dr. V. Rajan DM Hepatology 5 Dr. Kelika Prakash DM Organ Transplant Anesthesia and Critical Care 6 Dr. Bikrant Raghuvanshi DM Pediatric Hepatology 7 Dr. Kapil Chakarvarti MCh (HPB Surgery) 8 Dr. Rommel S MCh (HPB Surgery) 9 Dr. Nihar Mohapatra MCh (HPB Surgery) 10 Dr. Kumar Abhinav DM Hepatology 11 Dr. Swati Das PDCC Abdominal Imaging (Joind in IR) 12 Dr. Akshay Batra PDCC in Blood Banking and Immunohaematology 13 Dr. Sukanya Baruah PDCC in Blood Banking and Immunohaematology 14 Dr. Mohd Qurram Parveez PDCC in Liver Transplant Anesthesia 15 Dr. Ankur Sharma PDCC in Liver Transplant Anesthesia 16 Dr. Neha Nigam PDCC in Hepatopathology 17 Dr. Ayushi Agarwal PDCC in Hepatopathology 18 Dr. Mousumi Paul PDCC in Hepato-Virology 19 Dr. Pradheer Gupta PDCC in Hepato-Virology 20 Dr. Ankusha Yadav PDCC in HPB Interventional Radiology 21 Dr. Rohit Shriram Khisti PDCC in HPB Interventional Radiology 22 Dr. Sheetal Ganju PDCC in Pediatric Hepatology 23 Dr. Ruchika Kumar PDCC in Pediatric Hepatology 24 Ms. Harshita Tripathi PGCC Clinical Nutrition 25 Dr. Sumit Gupta PDCC in Liver Transplant Anesthesia 26 Dr. Atchya Arunkumar Mannan PDCC in Liver Transplant Anesthesia 27 Dr. Kishore Kumar PDCC in Liver Transplant Anesthesia 28 Dr. Nishant Singla PDCC in HPB Interventional Radiology 29 Dr. Naveen Kumar Condati PDCC in HPB Interventional Radiology 30 Dr. Jitender Singh PDCC Abdominal Imaging 31 Dr. Sujata Lall PDCC in Hepato-Virology 32 Dr. Abhijit Prakash Chougule PDCC in Hepatopathology 175

33 Dr. Anu Chawla PDCC in Hepatopathology 34 Dr. Mitali Singhal PDCC in Hepatopathology 35 Dr. Lakshmi Kumar Chalamarla PDCC Abdominal Imaging 36 Dr. Prayas Vats PDCC- Abdominal Imaging 37 Dr. Ritu Khosla PhD –Biomedical Sciences 38 Dr. Ashish Kumar Vyas PhD-Biomedical Sciences 39 Dr. Sukanta Das PhD-Biomedical Sciences 40 Dr. Aditi Varshney PhD-Biomedical Sciences Events and Conferences Year 2017 The Institute of Liver and Biliary Sciences, Deemed-to-be-University organised range of events out of which mostly are educational like CMEs, CPCs, workshops and conferences in all specialities/ Departments to enhance and update residents/students knowledge and knowhow on the subject. S N. Details of Events/Conferences Date Department 1 Induction Program 2017 02.01.2017 Academics 2 4th Convocation Day at ILBS 13.01.2017 Academics 3 CSPLD 2017 04.03.17-05.03.17 Pediatric Hepatology 4 World Kidney Day 09.03.2017 Nephrology 5 World Hepatitis Day 28.07.2017 Hepatology 6 ILBS ICON Oration by Dr. Pratap C. Reddy 07.08.2017 Academics 7 6th Ramalingaswami oration 08.08.2017 Pathology 8 Flow Cytometry Camp 19th -20th Aug17 Molecular and Cellular Medicine 9 Mammalian Cell Culture and Microscopy 1st -3rd March 2017 Molecular and Cellular Medicine 10 Biostatistics Workshop 16th-19th August 17 Statistics 11 Hepatobiliary imaging and Interventions 18.11.2017 Radiology 12 20th Hepatitis Day 04.12.2017 Hepatology Clinic Pathological Conferences in Year 2017 The Institute of Liver and Biliary Sciences, Deemed-to-be-University organised range of events out of which mostly are educational like CMEs, CPCs, workshops and conferences in all specialities/ Departments to enhance and update residents/students knowledge and knowhow on the subject. 176

Date Details of Events/Conferences Department Dr. Sundeep Singh Saluja, Professor March HPB Surgery Department of G.I. Surgery,G.B. Pant Hospital Dr. S.K. Yacha, Professor and Head Department of Pediartric April Pediatric Gastroenterology SGPGI Lucknonw Hepatology Dr. Ranjit Kumar Sahu, Assistant Professor, Department of Medical Oncology, May Oncology AIIMS, New Delhi June Resident Presentation By Sr. Resident Nephrology and Pathology Nephrology Dr. H. H. Nag, Professor Surgical August HPB Surgery Gastroenterol ogy. Department of G I Surgery, G B Pant Hospital Staff Faculty Designation Prof. S.K. Sarin Director Dr. Vijay Kumar Dean Dr. Seema Alam Controller of Examination Dr. B.C.Sharma Associate Dean (Reasearch) Dr. Archana Rastogi Associate Dean (Academics) Staff Designation Ms. Vandna Talwar Assistant Registrar Vanita Pal, Jr. Exceutive Ravindra S Jr. Exceutive 177

178 ILBS Residents

ILBS Faculty 179

College of Nursing 180

College of Nursing In view of the requirement for highly specialised nurse to undertake liver critical care and transplant care it was decided to start MSc Nursing and other post graduate diploma courses in GI and Liver Nursing. The College of Nursing, ILBS was started in 2016 and is the first of its kind in the country for Critical Care in Liver Diseases. MSc NURSING AT ILBS MSc Nursing programme at ILBS provides specialization in Medical Surgical Nursing with sub- specialization in Gastroenterology Nursing. The programme is recognized by Indian Nursing Council and Delhi Nursing Council.  Duration of the Programme: 2 yrs  Total student Intake per year: 15 Session for first batch of MSc Nursing commenced on 3rd October 2016 with 11 students. Session for second batch of MSc Nursing commenced on 1st of August 2017 with 13 students. Educational Activities The two year M.Sc Nursing programme will enable the graduate to practice as a nurse specialist in Liver and Biliary Sciences, in leadership roles and function effectively as nurse educator and manager It will help them to ddemonstrate skill in conducting nursing research and exhibit advanced competence in practice of nursing  The course is structured based on the guidelines given by the Indian Nursing Council.  Students begin their study in a blended format with classroom-based didactic courses building the theoretical and contextual background necessary for advanced practice nursing. 181

 This curriculum is of two years. Students will take 3-4 subjects per year.  All students are to participate in a hands-on practical during their entire course.  It concludes with a culminating research study.  Ongoing evaluation of students learning outcomes ensures that they have achieved the competencies consistent with professional standard. Admissions are done on the basis of Entrance Examination and Counselling. Out of the 129 applications received for 2017-19 batch, 3 male and 12 female candidates were shortlisted and joined the course. Major highlights of 2017 are :  Second batch of MSc Nursing students admitted.  University examination conducted for the first batch MSc Nursing.  Started departmental research activities. Other Education activities include the following:  Training for various course in Nursing.  Continuing Education of Nursing Staff.  Involving in recruitment of Nursing Staff  Research in Nursing Science. 182