B16F10 Cell Line

B16F10 Cell Line General Information Organism Mus musculus, mouse Cell Line B16F10 is a murine melanoma cell line from the C57BL/6J mouse. Description It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, then injecting them into fresh mice, a total of 10 times. B16F10 cells are highly metastatic and can form tumors and metastases post implantation into syngenic C57BL/6 mice or immunocompromised mice. Strain C57BL/6J Tissue Skin Disease Melanoma Morphology Mixture of spindle-shaped and epithelial-like cells Gender Unknown Primary No Product Frozen Format

Growth Mode Adherent Biosafety 1 Level Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Applications Cell culture / growth conditions, stable cell transfection, gene expression, protein expression, transient transfection Shipped in Dry ice Storage −196°C Temperature References J. Natl. Cancer Inst. 60: 1217-1222, 1978 Characteristics Images Isoenzyme NP, G6PD, MD, MPI, AST, LD and Pep B Analysis

Mycoplasma Negative Test Tumorigenic Yes, in syngeneic mice Comments Useful for metastasis study. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. Culture Conditions and Handling Thawing 1. Thaw the vial by gentle agitation in a 37°C water bath. To Frozen Cells reduce the possibility of contamination, keep the oring and cap out of the water. Thawing should be rapid (about 2 minutes). 2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. 3. For cells that are sensitive to DMSO is recommended that the cryoprotective agent be removed immediately. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5-7 minutes. 4. Discard the supernatant and resuspend cell pellet with the complete medium. 5. Incubate the culture in an appropriate atmosphere and temperature. Culture 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine Medium adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum.

Subculturing 1. Remove medium, and rinse with PBS without calcium and magnesium. 2. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. 3. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation The ratio of 1:10 is recommended. Ratio Medium Every 2 to 3 days Renewal Freeze Medium 93% culture medium + 7% DMSO> Culture 37°C Temperature Incubation 5% CO2 Condition