NGS Technology

NEXT GENERATION SEQUENCING SERVICE Next-generation sequencing 1 DNA Library Preparation 2 Clonal Amplification (NGS) technologies have progressive advantages in 1 23 terms of cost-effectiveness, unprecedented sequencing 45 speed, high resolution and accuracy in genomic analy- 3 Cyclic Array Sequencing ses, thus are playing an increasing important role in T fields of oncology and immu- C nology. G T 3' A The sequencing principles of 5' AA common sequencing instru- ments on the market: (1) first, T 3' construct a DNA template A 5' library. Obtain DNA library T 3' fragments (tens to hundreds A 5' of bases in length) by T 3' randomly breaking genomic DNA, or construct paired-end 5' fragments that control the distance distribution. Adapter A sequences were ligated to both ends of the DNA T 3' fragment and then denatured A A 5' to obtain a single-stranded A template library and immobi- A lized on a solid surface. (2) Second amplify the clones, A A which performed by one of A several methods, such as bridge PCR and emulsion 3' G A C A n n n z z PCR. (3) Then, forming DNA clusters or amplifying micro- A CG T GAn n n z z z CT GT 3' spheres of DNA cluster arrays A CT on a chip, performing a series C 3' 3' 3' G A C A of cyclic reaction operations G 3' CT GT using a polymerase\\ or ligase, 3' and monitoring the optical T events generated in each cycle biochemical reaction by 3' 5' 3' 5' G A n n n 5' z z z GA CA T A AA a microscopic detection syst- AAnnn z z z CAn n n z z z C T 3' CT GT AT T T em. Time series analysis of TG CC GT the resulting array images is 3' 5' 3' 5' A C GG C A performed to obtain sequenc- GA n n n z z z TAnnn z z z es of DNA fragments. These fragments are then assem- WHAT WE DO: Whole Transcriptome Sequencing (WTS) bled into longer contigs Service for Cancer according to certain computer Whole Genome Sequencing (WGS) algorithms. Service for Cancer Immune Repertoire Sequencing (Rep-seq) Service for Cancer Creative Biolabs uses the Whole Exome Sequencing (WES) advanced SuPrecision™ pl- Service for Cancer Bioinformatics Analysis Service atform to support researchers all over the world with their Targeted Sequencing Service for sequencing needs for cancer. Cancer Creative Biolabs Next Generation Sequencing Service © 2007 - 2019 Creative-Biolabs All Rights Reserved Email: [email protected] Tel: 1-631-381-2994