deamidation analysis

deamidation analysis • PtsrvTgehealhTyqerrMecuiuroroamaSiusrppeynseceldoaduluettibttsiaiooiycercnnaamppl,nsyrreoocooaesxstt.ftei,edfCipteinahnochtsestmia,oreprbapnmfruplf,oeitaedcdxcuaieueopltascnikocmottissnpsmtiehd-erttoaaacrivtytrcaeieesnoii,tnnsnbieofls,ealtn.upcteC,ie.rnodoonnprtmcaeuerloomgsmvleayoesoffnnefidsitcPe,iyafTfa,icfcMnieymadctsatmisoinivnonudecsnflsoauo(tgrPdagtaTbeehiMinn.lisiTtsctyh)iteaoys,rfee • DihDcnhaeetapoaarpmmgaeesiinddp, saamattdriiatoouiscnnrsi,animsangnidtgphdhguetlarupirfrterieacosdmaucuteliitccostisnaniocoocnifdfh/csnlaoo,nanrstgesvueseorpsrafettlbiocnaitognpivldraoeosrgltypeie,caciawnorlamhhagiycibcndthiinervofaiartpnenyht.qdopugbreolinuctttietlayiymn, si.ne

• Oxidation is a major degradation pathway in protein and peptide formulation and storage, and might cause potential problem in protein production, isolation, and purification. Several amino acids are susceptible to oxidation, including methionine, cysteine, histidine, tyrosine, tryptophan and phenylalanine. Besides, oxidation of certain amino acids can cause changes of conformation, binding affinity, stability, lifespan, immunogenicity, which consequently affect the safety and efficacy of therapeutic proteins. • Since deamidation and oxidation are typical degradation for therapeutic proteins, deamidation and oxidation are critical quality attributes (CQAs) and must be presented during drug discovery. • Creative Proteomics provides a high-sensitivity deamidation and oxidation analysis service to identification and quantification of deamidation and oxidation in accordance with relevant guidelines (ICH Q6B, PharmEu and USP<1047>). Our experienced scientists and professional analytic team will deploy different approaches to help you with deamidation and oxidation analyses, and meet the requirements of every stage of drug discovery.

• We Can Provide but Not Limited to: • Asparagine Deamidation analysis • Glutamine Deamidation analysis • Deamidation sites identification • Deamidation products quantification • Oxidation analysis • Oxidation sites identification • Oxidation product quantification • Glucose Unit (GU) values determination • Technology Platform of Deamidation and Oxidation Analysis Service: • Creative Proteomics provides efficient and accurate deamidation and oxidation analysis services through several techniques, including ion exchange chromatography (IEC), capillary isoelectric focusing (cIEF), hydrophobic interaction chromatography (HIC), liquid-chromatography-tandem mass spectrometry (LC-MS/MS), etc. • Both asparagine and glutamine deamidation can be rapidly analyzed by ion IEC and LC-MS/MS. And identification of oxidation sites can be determined by HIC, reverse phase- HPLC coupled with Fabricator digestion, and mixed mode size0exclusion chromatography (SEC). Creative Proteomics offers simultaneous identification and quantification of deamidation and oxidation by bottom-up LC-MS with ultrafast tryptic digestion. Pharmaceutical products will be digested into peptides by a 5 min tryptic digestion method. Then the peptides will be separated with nano-LC before subject to mass spectrometer.