Vascular Permeability Assay In Vivo

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• The vascular permeability assay In Vivo at Creative Proteomics, also known as Miles assay, is a well-established method to determine vascular permeability In Vivo. This method is based on the intravenous injection of Evans Blue, which is a dye with high affinity for albumin in mouse models. Under physiological conditions, albumin does not cross the endothelial barrier and Evan Blue bound albumin restricted within blood vessels. In pathologic conditions that promote increased vascular permeability, endothelial cells partially lose their close contacts and the endothelium becomes permeable to small proteins such as albumin. Thus, since blood vessels start to leak protein and also the Evans Blue that is bound to albumin, Evans Blue will appear in the organs with increased permeability. Whereas, a healthy endothelium prevents the Evans Blue crossing the blood vessels and accumulating in organs. Organs with increased permeability will show significantly increased blue coloration compared to organs with intact endothelium. The extent of vascular permeability can be quantified by simple visualization or by quantitative measurement of the dye incorporated of the tissue or animal.

• The simplicity and quantitative characteristics of this method makes it a great advantage over other permeability assays. This assay is also a powerful and versatile method to determine the effect of different compounds on vascular permeability. The results of this assay can be accompanied by molecular studies, which lead a further understanding of vascular permeability.