ubiquitin mass spectrometry

ubiquitin mass spectrometry • Ubiquitin is a small (8.6 kDa) regulatory protein and ubiquitination is an important post-translational modification, in which ubiquitins are attached to a substrate protein. This process contains three main steps: activation, conjugation, and ligation and requires three types of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s).

• The ubiquitin-proteasome system mediates 80%-85% protein degradation in eukaryotes. In addition to participating in protein degradation, ubiquitination is involved in almost all life activities, including the cell cycle regulation, proliferation, apoptosis, differentiation, transcriptional regulation, gene expression, transcriptional regulation, signal transmission, damage repair, inflammatory immunity. Ubiquitination is related to the pathogenesis of diseases, such as cancer and cardiovascular diseases. Therefore, it has become a new target for research and development of new drugs.

• Overview of Protein Ubiquitination Analysis • Creative Proteomics has already developed a professional platform for ubiquitin-proteomics analysis services to help you address research problems of ubiquitination identification and quantification. Through comprehensive protein ubiquitination analysis services, including protein extraction, digestion, peptide enrichment, LC- MS/MS analysis, data analysis and bioinformatics analysis, we can quickly, accurately and efficiently analyze the changes of various disease-related protein modifications to meet the needs of biomarker identification and drug target screening.