3D Invasion Assay

3D Invasion Assay • In experimental cellular biology, invasion is defined as disruptive, proteolytic, cellular movement through three-dimensional barriers. Despite intense study, the complex and heterogenous mechanisms of invasion and metastasis remain imperfectly understood and several mechanisms exist in collective or individual invasion of cancer cells. Thus, there is a need to establish robust, reproducible assays to target the process and get a detailed analysis of invasion. The three- dimensional (3D) spheroid invasion assay is a simple and convenient method to research cell invasion, which is based on the uniform and self-assembling 3D tumor spheroids.

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• This method to study cell invasion can provide great advantages over other in vitro cell invasion assays. In three-dimensional (3D) spheroid invasion assay, the tumor cells are organized into a 3D culture, which could mimic a tumor micro-region or micro-metastasis. Another advantage of this assay is high reproducibility, since the spheroids are highly reproducible in size and this assay is performed in the same plate without the need to move the spheroids to another plate. In our laboratory, the application of the latest technologies of automated image analysis enables the high throughput analysis of cell invasion.