Aptamer-Development-PLATFORM

Aptamer COMPLEMENT THERAPEUTICS Development PLATFORM Creative Biolabs offers comprehensive one-stop DNA or RNA aptamer development services against different targets. https://www.creative-biolabs.com/complement-therapeutics/

About Us Creative Biolabs is a leading custom service provider with extensive experience in antibody production and engineering. We have For more than 10 years, recently built a one-stop aptamer development platform. We are we have been bringing confident in screening aptamers with high affinity against different values to our valuable forms of targets, such as small organic, peptide, protein, cell, and customers by providing bacteria. high-quality products and services quickly and at We provide a broad portfolio of laboratory services for aptamer competitive prices. development: • One-stop Aptamer In Vitro Selection Service • Aptamer-based Conjugation Services • Aptamer Modification Services • Aptamer Optimization Services • Aptamer Characterization Services

01 02 Outline One-stop Aptamer In Vitro Aptamer-based 03 Selection Service Conjugation Services Aptamer Modification 04 05 Services Aptamer Optimization Aptamer Characterization 06 Services Services Our Advantages

1 One-stop Aptamer In Vitro Selection Service CREATIVE BIOLABS INC. APTAMER IN VITRO SELECTION STEP 03 STEP STEP Identification and STEP STEP 01 02 Characterization of 04 05 Monoclonal Aptamer Library Enrichment Selection of Polyclonal Optimization of the Delivery and (SELEX) Aptamers 1. Synthesize and purify the top 5 Best Aptamer Report monoclonal aptamer oligo candidate(s) 1. Chemically synthesize and 1. Produce 5 pools from phases 1 2. Quantitatively test each candidate 1. Truncate the best aptamer (1 1. Supply 5 nanomoles of the modify single-stranded DNA library with indexed primers for NGS for Kd affinities by SPR, BLI, ELISA, aptamer) and test the binding best monoclonal aptamer or or RNA library sequencing Microscopy, Flow Cytometry, etc. properties of the variants. modified variant. 2. Iterative rounds of target 2. NGS sequencing (105 to 106 reads 2. Provide suggestions for chemical 2. Deliver project report screening (positive and negative), per round) and identifying up to 20 modification(s) of the best variant, containing all sequences from including small organic, peptide, sequences. such as modification of the NGS sequencing. protein, cell, and bacteria. backbone to improve the nuclease resistance; conjugation to a fluorophore…

2 Aptamer-based CREATIVE BIOLABS INC. Conjugation Services Because of the high targeting specificity, aptamer-based conjugates can improve the efficacy and biological distribution of molecules, reducing the accumulation in non-target tissues that could result in off-target effects. At Creative Biolabs, we can conjugate various interesting drugs, antibodies, nanomaterials with aptamers and study them to provide effective and safe aptamer-based conjugates for researchers worldwide. OUR APTAMER-BASED CONJUGATE DEVELOPMENT SERVICES INCLUDE: • Aptamer-therapeutic Oligonucleotide Conjugates Development Service • Aptamer-antibody Conjugates Development Service • Aptamer-drug Conjugates Development Service • Aptamer-cholesterol Conjugates Development Service • Aptamer-nanoparticles Conjugates Development Service • Bivalent Aptamer Development Service • Aptamer-based Biosensor Development Services

3 Aptamer Modification Services CREATIVE BIOLABS INC. Equipped with state-of-the-art technologies and rich expertise in aptamer development, we provide a full range of aptamer modification services for global clients. We have completed several challenging aptamer modification projects and accumulated extensive experience. According to clients’ specific requirements, we can optimize aptamer modification solutions to improve their performance, such as stability or specificity. OUR APTAMER-BASED MODIFICATION SERVICES INCLUDE: • 2' Fluoro Modification • 2'-O-methyl modification • 3' Inverted Thymidine Modification • Fluorescence Modification • Biotin Modification • Digoxigenin Modification • Phosphorylation Modification • Spacer Modification • Amino and thiol Modification

02 4 Aptamer Optimization Services CREATIVE BIOLABS INC. A number of therapeutic aptamers are currently evaluated in preclinical and clinical trials. However, although sometimes successful, the practical applications of aptamer are usually much less efficient and successful than initially predicted. As a pioneer and the undisputed global leader in novel drug discovery and manufacturing, Creative Biolabs has earned a good reputation in supporting aptamer development and optimization. Our professional scientists are confident in offering domestic and international customers a range of tailored strategies and services to optimize aptamers. OUR APTAMER OPTIMIZATION SERVICES INCLUDE: • Aptamer basic sequences optimization • Aptamer structure stabilization • Aptamer hydrophobic/hydrophilic portions optimization • Chemical modifications • SELEX optimization • Post-SELEX optimization

02 CREATIVE BIOLABS INC. 5 Aptamer Characterization After several rounds of screening by SELEX, the aptamers Services of interest with target molecule specificity will be obtained. To ensure the efficiency of therapeutic applications, these selected aptamers must be highly stable, of moderate length, and have good pharmacokinetic properties. Therefore, specific assays are needed to characterize and evaluate the properties of aptamers. Usually, aptamer characterization involves structure determination, binding kinetics measurement, and specificity test, etc. Based on an advanced technology platform, our professional scientists provide comprehensive aptamer characterization services to evaluate every aptamer. OUR APTAMER CHARACTERIZATION SERVICES INCLUDE: • Tertiary structure analysis • Binding affinity measurement • Thermodynamic profiling • Competition assays

07 CREATIVE BIOLABS INC. 6 Our Advantages Service Features • Extensive experience and advanced biotechnologies • Fully customizable experimental design to meet your R&D timeline and budget • Best quality with competitive price • Best after-sale service Why Us • 10 years+ of exploration & development • Full range of one-stop services for aptamer development • Specially modified libraries enable the production of aptamers with high affinity and specificity • Fully customizable project procedure • Your best partner in aptamer-based diagnosis and treatment

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