Design of Bispecific Antibody

Recombinant Anti-Ad fiber knob x Anti-CD40 Bispecific Antibody (Fab-scFv-Fc) Cat. No.: FABVC-002 This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Introduction Recombinant Anti-Ad fiber knob x Anti-CD40 Bispecific Antibody (Fab-scFv-Fc) requires expression of three polypeptide chains, encoding two heavy chains (Fc-containing chains) and one light chain, Targets respectively. The Fab of anti-Ad fiber knob antibody variable domain is fused to the Fc-knob domain with a Type hinge region and the same as the scFv of anti-CD40 to the Fc-hole domain, or vice versa. The Fab-Fc and Species Reactivity scFv-Fc fragments can be paired via the interaction of the Fc regions. This BsAb can retarget of adenoviral Target 1 vectors to tumour cells. It can be used in cancer gene therapy to transfer a therapeutic gene into tumour Host Animal 1 cells aiming at selective and efficient cell killing. Target 2 Host Animal 2 Ad fiber knob & CD40 Application Related Disease Fab-Fc Fusion Proteins Research Area Human Ad fiber knob Mouse CD40 Mouse FuncS; Promising therapeutic agent Acute myeloid leukaemic (AML) Oncology; Gene therapy BACKGROUND SUITE 203, 17 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-416-1478 Fax: 1-631-207-8356 © Creative Biolabs All Rights Reserved Page 1 of 2

Target 1 Ad fiber knob Alternative Names Description Ad fiber knob; Adenovirus fiber knob; adenovirus fiber protein; knob domain of Ad fiber Target 2 In adenovirus, fiber protein is used to attach the virus to the host cell surface. Knob is a domain of fiber Gene ID protein, which is a long and thin shaft. Ad fiber knob has an important role in fiber protein structure and UniProt ID function. It mediates the interaction of adenovirus with the host cells. Alternative Names Description CD40 958 P25942 CD40; CD40 molecule; p50; Bp50; CDW40; TNFRSF5 CD40 (Cluster of differentiation 40), also referred to TNFRSF5, is a 48 kDa phosphorylated glycoprotein encoded at the chromosome of 20q13.12 and contains 277 amino acids. This type I transmembrane protein is originally characterized on B cells, and later found on platelets, monocytes, macrophages and dendritic cells, as well as on non-hematopoietic cells including fibroblasts, myofibroblasts, epithelial and endothelial cells. The CD40 receptor on antigen-presenting cells of the immune system and important for mediating a variety of immune and inflammatory processes including memory B cell development, T cell-dependent immunoglobulin class switching, and germinal center formation. CD40 has a ligand known as CD40L or CD154, a type II transmembrane protein, composed of a sandwich extracellular structure and belonging to the TNF superfamily. SUITE 203, 17 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-416-1478 Fax: 1-631-207-8356 © Creative Biolabs All Rights Reserved Page 2 of 2