pmal c5e

Plasmids Design and Construction Plasmid vector construction is an essential step in molecular biology. The plasmid design and construction platform developed by Creative Biostructure is aimed to deliver application-specialized plasmids design and construction services. Our services include constitutive and inducible vectors for various expression systems. We can also assist you in case your required plasmid vector is not available. Services • Sequence analysis • Vector design • Gene synthesis • PCR cloning • DNA fragment assembling • DNA sequencing • Site-directed mutagenesis • Plasmid production Following is a list of vectors available in different expression systems: Expression Vectors for Bacterial Systems pGS-21a pETDuet-1 pCDFDuet- pCOLADue pET-3a pET-3b 1 t-1

pET-3c pET-3d pET-9a pET-11a pET- pET-11c 11b pET-11d pET-14b pET-15b pET-16a pET- pET-19b 17b pET- pET- pET- pET- pET- pET- 20b(+) 21a(+) 21b(+) 20d(+) 22b(+) 23a(+) pET- pET- pET- pET- pET- pET- 24a(+) 24b(+) 24c(+) 24d(+) 25b(+) 26b(+) pET- pET- pET- pET- pET- pET- 27b(+) 28a(+) 28b(+) 28c(+) 29a(+) 29b(+) pET- pET- pET- pET- pET- pET- 29c(+) 30a(+) 30b(+) 30c(+) 31b(+) 32a(+) pET- pET- pET- pET- pET- pET- 32b(+) 41a(+) 41b(+) 41c(+) 42a(+) 42b(+) pET- pET- pET- pET- pET- pET- 42c(+) 43.1a(+) 43.1b(+) 45b(+) 50b(+) 51b(+) pET- pGEX-2TK pGEX-4T-1 pGEX-4T- pGEX- pGEX-5X- 52b(+) 2 4T-3 1 pGEX-5X-2 pGEX-5X-3 pGEX-6P-1 pGEX-6P- pGEX- pMAL-c4x 2 6P-3 pMAL-c5E pMAL-c5x pMAL-p5E pMAL-p5G pMAL- pQE-1 p5X Expression Vectors for Yeast Systems pPIC3.5k pPIC9 pPIC9K pPICZA pPICZB pPICZC pPICZakoh pPICZakoh pPICZakoh pESC-TRP pESC- pESC-HIS aA aB aC URA Expression Vectors for Insect Systems

pBacPAK8 pBacPAK9 pAcG2T pAcHLTA pAcHLT pAcHLTC pAcGHLTA pAcGHLTB pAcGHLTC pAcSG2 B pBAC-1 pFastBac 1 Expression Vectors for Mammalian Systems pcDNA3.1(+) pcDNA3.1(-) pcDNA3.1(+)_myc pcDNA3.1(+)_myc -HisA -HisB pCI-Neo pcDNA3.1/Zeo( pcDNA3.1(+)_myc pcDNA3.1(-)_myc +) -HisC -HisA pcDNA3.1/Zeo( pCMV-3Tag-1a pcDNA3.1(-)_myc pcDNA3.1(-)_myc -) -HisB -HisC pCMV-3Tag-2a pCMV-3Tag-3a pcDNA3.1+C-DYK pcDNA3.1+C-HA pCMV-3Tag-4a pcDNA3.1+C- pcDNA3.1+C-6His pcDNA3.1+C-Myc eGFP pcDNA3.1+P2A- pcDNA3.1+N- pcDNA3.1+N-DYK pcDNA3.1+N-HA eGFP eGFP pcDNA3.1+P2A pET-42(+) pcDNA3.1+N-6His pcDNA3.1+N-Myc pET-43.1a(+) pET-43.1b(+) pcDNA3.1+N- pcDNA3.1/Hygro( GST(TEV) +) pET-45b(+) pET-52b(+) pcDNA3.1/Hygro( pcDNA3.1+C-DYK- -) P2A pGEX-2TK pGEX-4T-1 pcDNA3.1+N-DYK- pGEX-4T-1- P2A M(RBS) pGEX-4T-2 pGEX-5X-2 pGEX-5X-1- pGEX-5X-1- H(RBS) M(RBS) pGEX-5X-3 pGEX-6P-1 pGEX-6P-1- pQE-60 H(RBS)

pGEX-6P-2 pMAL-c5E pGS-21a pETDuet-1 pMAL-c5x pMAL-p5E pMAL-p5G pCDFDuet-1 Creative Biostructure performs the custom plasmid construction from design to production. We have successfully designed and constructed numerous plasmid vectors. With our extensive experiences, we are certain that we can help with your research projects, please feel free to contact us for a detailed quote Reference: 1. S. M. Yoo, et al. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature Protocols, 8: 1694–1707 2. J. W. Bok, et al. (2012). Fast and easy method for construction of plasmid vectors using modified Quickchange mutagenesis. Methods Mol. Biol., 944:163-174 3. S. Kwon, et al. (2015). Comparative genomics and experimental evolution of Escherichia coli BL21(DE3) strains reveal the landscape of toxicity escape from membrane protein overproduction. Scientific Reports, 5:16076