Etrolizumab

Etrolizumab • Recombinant monoclonal antibody to integrin alpha 4 beta 7. Etrolizumab is a humanized monoclonal antibody designed for the treatment of inflammatory bowel disease.

• (A) Upper panels: Flow cytometry of peripheral blood mononuclear cells (PBMCs) from patients with Crohn's disease (CD), ulcerative colitis (UC), and control donors (CON) treated with etrolizumab-s for 24 h at 4 and 37°C. Left: Representative flow cytometry. The frequency of 9D8+ staining on CD4+ T cells is indicated. Right: Quantitative flow cytometry showing surface expression of 9D8 at 37°C relative to expression at 4°C (n = 5 per group). Lower panels: Representative (left) and quantitative flow cytometry (right) of cells cultured at 4 and 37°C without etrolizumab-s treatment. (B) Flow cytometry of PBMCs from CD, UC, and CON donors treated with AF-647-labeled etrolizumab-s. Left: Representative flow cytometry. The frequency of etrolizumab-s+9D8- cells among CD4+ T cells is indicated. Right: Quantitative flow cytometry showing the proportion of these cells at 4°C in relation to 37°C (n = 5 per group). (C) Quantitative flow cytometry of PBMCs treated with AF647-labeled etrolizumab-s at 4 or 37°C and subsequently subjected to acid wash (left) or not (right). n = 5 per group; data are normalized to expression at 37°C. ∗ ∗ ∗ p < 0.001; ns – not significant.