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CRISPR Application
In CRISPR/Cas9 system, there are two critical parts: sgRNA sequence and Cas9 sequence. These two sequence could be cloned into separated plasmids or combined into one plasmids.

Considering the limited utility of protoplast system, it’s also available to transfect preassembled complexes of purified Cas9 protein and synthesized sgRNA.

https://www.creative-biogene.com/crispr-cas9/

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CRISPR Application

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