CLS_SIELC_Catalogue

HPLC/UHPLC Columns Innovative manufacturing of mixed-mode HPLC columns Primesep® HPLC columns are specifically designed for mixed-mode HPLC/UHPLC Columns separations and are capable of separating a tremendous range of compounds by different separation modes based only upon mobile phase selection. A wide range of HPLC columns with id’s from 0.5 mm to 22 mm are available. PrimeSep® Phase Particle Size Pore Size ID Lengths Separation Typical (µm) (Å) (mm) Modes Application *A *100 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 Reversed-phase interac- Strongest acidic phase for weak bases, *200 0.5, 0.25 tion and cation-exchange AA, metals *C *500 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 Reversed-phase interac- Strong acidic phase for weak, medium AB 0.5, 0.25 tion and cation-exchange bases, AA, metals B 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 Reversed-phase and Medium acidic phase for strong bases, B2 0.5, 0.25 cation-exchange dibases D 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 Reversed-phase, cation Strong bases, dibases SB 0.5, 0.25 exchange and complex- N Strong bases, dibases, polybases P 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 formation 0.5, 0.25 Separation of complex mixtures of polar Reversed-phase and anionic and cationic compounds 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 cation-exchange 0.5, 0.25 Strong basic column for use with TFA, Reversed-phase, anion- phosphoric or perchloric acid solutions in a 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 exchange and cation 0.5, 0.25 exchange mobile phase Weak basic column for ammonium acetate 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 Reversed-phase and 0.5, 0.25 anion-exchange buffered solution Small hydrophobic and acidic compounds. 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 Reversed-phase and 0.5, 0.25 anion-exchange Low MW plasma components and bio fluids 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 Reversed-phase and 0.5, 0.25 anion-exchange Neutral and acidic compounds 5, 10 100, 300 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 Reversed-Phase, Basic and polar compounds 0.5, 0.25 anion-exchange and ion- Choose for separation of structural isomers 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 exclusion of aromatic compounds. Weak, medium 0.5, 0.25 aromatic bases Normal-phase, cation- exchange and HILIC Reversed-phase, pi-pi and strong cation-exchange *Choose the correct column by their functional group acidity to match the pKa value of your basic compounds. Primesep Phase About 50% of embedded acidic groups ionized at transition pH value A Ionized in all working pH 100 pH 1 200 pH 2 C pH 3.5 500 pH 5 Primesep A, 100, 200, 500 Primesep C Primesep B, B2, D, SB Primesep D Primesep P 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN H-1 LIFE SCIENCE

Colonnes HPLC/UHPLCColonnes HPLC/UHPLC Obelisc™ Obelisc™ columns, Obelisc R with reversed-phase characteristics and Obelisc N with normal-phase characteristics, separate polar and non-polar compounds using multiple separation mechanisms. Based on Liquid Separation Cell (LiSC ™) technology, they offer a new approach to separate a variety of small molecules. Buffer concentration, buffer pH, and organic modifier concentration are three orthogonal parameters of the mobile phase that allow adjustment of column properties to separate complex mixtures. ASK US ABOUT THE NEW SIELC PEI COLUMN! Two Obelisc Columns with Liquid Sepataion Cell Properties Phase Particle Size Pore Size ID Obelisc™ Phase Typical (µm) (Å) (mm) Lengths Characteristics Application R 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, 250, 150, 100, 50, 25, 10 Cationic groups separated from Analysis of complex mixture of acids, 0.5, 0.25 250, 150, 100, 50, 25, 10 anionic groups by hydrophobic chain basis, amino acids and neutral compounds N 5, 10 100 22, 10, 4.6, 3.2, 2.1, 1, Anions separated from the cationic Anlaysis of polar and charged analytes 0.5, 0.25 groups by hydrophilic chain SHARC™ SHARC™ columns are the first commercially available columns with separation based primarily on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding is an interaction (attraction) of bound hydrogen atoms to the molecules with electronegative atoms such as oxygen, nitrogen, fluorine. SHARCTM columns are available in all standard dimensions: Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm Particles: 10 µm, 5 µm, 3 µm Pores: 100 A The SIELC guard column is a uniquely designed direct insert column that does not require any additional holders and allows zero dead volume connections with the column. Guards are available for all packing materials. The packing layer is 10 mm long and is available in 1 mm, 2 mm, 3 mm, and 4 mm internal diameters. Contact your sales representative for more information H-2 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE