Grace catalogue

Customer Service About Canadian Life Science Au suject de Canadian Life Science Founded in 1997, Canadian Life Science (CLS) has steadily grown Fondée en 1997, Canadian Life Science (CLS) n’a cessé de croître to Canada’s leading supplier of LC/GC columns, chromatography pour devenir un chef de file canadien dans la vente de colonnes and dissolution accessories as well as 2d long-term sample HPLC / GC, d’accessoires de chromatographie et dissolution, storage tubes and racks for bio-banking and compound ainsi que dans la vente de tubes et systèmes d’entreposage long management. terme pour échantillons. For over 22 years, CLS has provided customers with unparalleled Depuis plus de 22 ans, CLS offre à ses clients un service et service, expertise and a wide selection of quality and cost- une expertise inégalés, en plus d’un large éventail de produits effective products that offer maximum flexibility to solve your économiques de qualité offrant une flexibilité maximale afin de analytical challenges. CLS is your foundation for what matters. résoudre vos problèmes analytique. CLS est votre pilier pour ce qui compte. Everyone is accessible to you Nous sommes très accessible We believe that our customers like our personal touch. Nous avons une approche personnelle et nous répondons à tous We take your calls personally, not a computer, not voicemail . les appels lorsque vous téléphonez au bureau chef. Nous n’avons We have dedicated inside customer service staff who care, and pas de boîte vocale. take care for specific areas. We have knowledgeable outside staff Notre personnel est dévoué au service à la clientèle et ce à based in most of the larger cities accross Canada. travers le Canada. Si vous désirez parler directement à notre We supply world class products Directrice de comptes au Québec, vous pouvez contacter Canadian Life Science distributes for top-quality manufacturers. Genevieve Lemieux au 514.428.8034. Our product offering has been chosen in a way that will give you Nous choisissons les meilleurs produits au monde the maximum flexibility solving your analytical challenges. En tant que distributeur nous avons l’avantage de choisir les Technical Support meilleurs fournisseurs et notre gamme complète de produit vous Our technical staff is happy to respond to customer enquiries. donne l’embarras du choix. We can also supply extensive application information from one Support Technique of the world’s largest data bases. Notre équipe technique se fait toujours un plaisir de vous aider à Plan the best delivery faire le meilleur choix pour vos applications. When you decide to purchase a product from Canadian Life Service et logistique Science our customer service department will find the fastest Lorsque vous placez une commande chez nous, nous nous and most economical way to send your order to you. For assurons de choisir le moyen le plus efficace et économique your convenience we have two warehouse locations, one in d’acheminer la marchandise. Edmonton, AB and one in Peterborough, ON. Nous avons deux entrepôts dont un en Alberta pour servir nos We’re glad to help clients de l’Ouest et un en Ontario pour servir nos clients de l’Est. We are never too busy to take your call. C’est un plaisir de vous servir Try us. You’ll notice the difference. Nous ne sommes jamais trop occupés pour vous répondre. Canadian Life Science, friendly people to do business with! Essayez-nous et vous verrez la différence. Il est agréable de faire affaire avec nous! I-22 CANADIAN Proud member of the Chrom4 buying group Fier membre du groupe dʼacheteur Chrom4 LIFE SCIENCE 1 888-226-2775 :: [email protected] :: www.lifescience.ca

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC USP Chromatographic Columns Listing Description Our Brand L1 Octadecyl silane (ODS or C18) chemically bonded to porous or non-porous silica or ceramic micro-particles, ACE C18, ACE Excel C18, ACE SuperC18, ACME C18, 1.5 to 10 µm in diameter, or a monolithic rod ACME F5/C18, ACME AMIDE/C18, ACME PLUS, HALO C18, HALO Peptide ES-C18, Inertsil (ODS, ODS-2, ODS-3, ODS-4, L2 Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid ODS-SP) Inertsil ODS-P, Inertsil ODS-HL InertSustain C18, spherical core, 30 to 50 µm in diameter InertSustain AQ-C18, InertSustainSwift C18 Grace Alltech Pellicular C18, ECONO PREP ODS L3 Porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod ACE Silica, ACME Silica, HALO HILIC, Inertsil SIL-100Å, Inertsil SIL-150Å, Inertsil WP300-SIL L4 Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm in diameter Grace Alltech Pellicular Silica, ECONO PREP SIL L5 Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm in diameter Please enquire L6 Strong cation-exchange packing-sulphonated fluorocarbon polymer coated on a solid spherical core, 30 to 50 µm Adsorbosphere XL SCX, Partisil SCX in diameter L7 Octylsilane (C8) chemically bonded to totally or superficially porous silica particles, 1.5 to 10 µm in diameter, ACE C8, ACE Excel C8, ACME C8, HALO C8, Inertsil (C8, C8-3, or a monolithic silica rod C8-4), InertSustain C8, InertSustainSwift C8 L8 An essentially monomolecular layer of aminopropylsilane (NH2) chemically bonded to totally porous silica gel Inertsil NH2, InertSustain NH2 support, 1.5 to 10 µm in diameter, or a monolithic silica rod. L9 Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, Inertsil CX, Partisil SCX, Partisphere SCX 3 to 10 µm in diameter L10 Nitrile groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod ACE CN, ACE Excel CN-ES, ACME CN, HALO ES-CN, HALO Peptide ES-CN, Inertsil CN-3, InertSustain Cyano ACE Phenyl, ACE Excel Phenyl, ACME Phenyl, HALO Biphenyl, L11 Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod HALO Diphenyl, HALO Phenyl-Hexyl, Inertsil (Ph, Ph-3), InertSustain Phenyl, InertSustain Phenylhexyl L12 A strong anion-exchange packing made by chemically bonding a quartenary amine to a solid silica spherical core, Pellicular SAX 30 to 50 µm in diameter L13 Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameter Adsorbosphere TMS, Adsorbosphere XL C1, Allsphere C1, APEX C1 L14 Silica gel having a chemicallly bonded strongly basic quaternary ammonium anion-exchange coating, 5 to 10 µm Adsorbosphere SAX, Adsorbosphere XL SAX, Allsphere SAX, in diameter Macrosphere 300 SAX, Inertsil AX, Nucleosil SB, Partisil SAX, Partisphere SAX, Platinum SAX, Vydac 3021C 20 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide USP Chromatographic Columns Listing Description Our Brand L15 Hexylsilane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter Allsphere C6, Chomegabond C6, Grom-Sil Hexyl-1 HPLC/UHPLC Column Selection Guide L16 Dimethylsilane chemically bonded to porous silica particles, 5 to 10 µm in diameter Adsorbil C2, Chromegabond C2, Nucleosil C2, Polygosil C2 Alltech OA-1000, Alltech OA-2000, Alltech IOA-1000, Alltech IOA- L17 Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the 2000, Hamilton HC-75H, Hamilton PRP-X200, Hamilton PRP-X300, hydrogen form, 6 to 12 µm in diameter Nucleogel Sugar 810H, Nucleogel ION 300 OA, Shodex IC Y-521, Shodex RSpak KC-811, Shodex SUGAR SH1011, Shodex SUGAR SH1821, Shodex SC1011, Shodex SC1211, Shodex SC1821 L18 Amino and cyano groups chemically bonded to porous silica particles, 3 to 10 µm in diameter Adsorbosphere PAC, Chromegabond A/CN, Legacy L18, Partisil 10 PAC, Partisphere PAC Hamilton HC-40,Hamilton HC-75 Ca, Nucleogel Sugar Ca, L19 Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the Nucleogel Sugar 810 Ca, Shodex SUGAR SC1011, calcium form, 5 to 15 µm in diameter Shodex SUGAR SC1211, Shodex SUGAR SC1821, Shodex USPpak MN-431 L20 Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10 µm in diameter, or a Inertsil Diol, Nucleosil Diol, Shodex PROTEIN KW-800 series, monolithic silica rod Shodex KW-400 series Nucleogel RP, Shodex RSpak DS-413, Shodex RSpak DS-613, L21 A rigid, spherical styrene-divinylbenzene copolymer, 3 to 30 µm in diameter Shodex RSpak RP18-413, Shodex RSpak RP18-415, Shodex RSpak RP18-613 Alltech Anion Exclusion, Nucleogel SCX, Shodex CXpak P-421S, Shodex IC Y-521, Shodex RSpak DC-613, Shodex RSpak KC-811, L22 A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, 5 to 15 µm in diameter Shodex SUGAR KS-800 series, Shodex SUGAR SC1011, Shodex SUGAR SC1211,Shodex SUGAR SC1821, Shodex SUGAR SP0810, Shodex SUGAR SZ5532, Shodex USPak MN-431 L23 An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quartenary ammonium groups, Allsep, Nucleogel SAX, Shodex IEC QA-825 7 to 12 µm in size L24 Polyvinylalcohol chemically bonded to porous silica particles, 5 µm in diameter Please enquire Packing having the capacity to separate compounds with a molecular weight range from 100-5000 Da(as deter- L25 mined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate Shodex OHpak SB-802HQ, Shodex OHpak SB-802.5HQ, resin base, cross-linked with polyhydroxylated ether (surface contained some residual carboxyl functional groups) Shodex SB402.5 was found suitable ACE C4-300, ACE C4, ACE Excel C4, HALO Protein C4, Inertsil L26 Butyl silane chemically bonded to totally porous or superficially porous silica particles, 1.5 to 10 µm in diameter C4, Inertsil WP300-C4, Vydac 214ATP, Vydac 214MS, Vydac 214TP, Vydac C4 L27 Porous silica particles, 30 to 50 µm in diameter Daisogel L28 A multifunctional support, which consists of a high purity, 60 Å, spherical silica substrate that has been bonded with Alltech mixed mode C8/anion, Legacy L28, ProTec C8 a cationic exchanger, sulfonic acid functionality in addition to a convention reversed phase C8 funcionality 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 21 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC USP Chromatographic Columns Listing Description Our Brand L29 Gamma alumina, reverse-phase, low carbon percentage by weight, alumina-based polybutadiene spherical par- Gammabond ARP-1, Gammabond Alumina Potency ticles, 5 µm in diameter with a pore volume of 80 Å L30 Ethyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter APEX Prepsil C2, Chromegabond C2-E, Nucleosil C2 A hydroxide selective, strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core Please enquire L31 of 8.5 µm macroporous particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with CHIRALPAK WH, Nucleosil Chiral-1 55% divinylbenzene L32 A chiral ligand-exchange resin packing-L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10 µm in diameter L33 Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spheri- Inertsil WP 300 Diol, Macrosphere 150Å, Shodex PROTEIN cal, silica-based, and processed to provide pH stability KW-800 series, Shodex KW-400 series L34 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead Nucleogel Sugar Pb, Shodex SUGAR SP0810 form, 7 to 9 µm in diameter L35 A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase Please enquire having a pore size of 150 Å L36 A 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5 µm aminopropyl silica L-Phenylglycine, Nucleosil Chiral-3 L37 Packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000 Da. It is a Shodex OHpak SB-803 HQ, Shodex SB403-4E polymethacrylate gel L38 A methacrylate-based size-exclusion packing for water-soluble samples Shodex SB400 series, Shodex OHpak SB-800HQ series L39 A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin Shodex SB400 series, Shodex OHpak SB-800HQ series, Shodex ODP2 HP, Shodex RSpak DM-614 L40 Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 3 to 20 µm in diameter CHIRALCEL OD, CHIRALCEL OD-H L41 Immobilized 1-acid glycoprotein on spherical silica particles, 5 µm in diameter CHIRALPAK AGP L42 Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5 µm in diameter Chromegabond PSC, HiChrom RPB 22 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide USP Chromatographic Columns Listing Description Our Brand L43 Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 µm in diameter ACE C18-PFP, ACME F5/C18, HALO PFP, HPLC/UHPLC Column Selection Guide InertSustain PFP, NUCLEODUR PFP, L44 A multifunctional support, which consists of a high purity, 60 Å, spherical silica substrate that has been bonded with NUCLEOSHELL PFP a cationic exchanger, sulfonic acid functionality in addition to a convention reversed phase C8 funcionality Chromegabond RP-SCX, Legacy L44 L45 Beta cyclodextrin, R,S-hydroxypropyl ether derivative, bonded to porous silica particles, 3 to 10 µm in diameter Nucleodex Beta OH, Nucleodex Beta PM, Shodex ORpak CDBS-453 L46 Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalized latex beads, Please enquire about 9 to 11 µm in diameter L47 High capacity anion-exchange microporous substrate, fully functionalized with a trimethylamine group, Hamilton PRP-X100, Hamilton PRP-X110, Hamilton RCX-10, 8 µm in diameter Hamilton RCX-30 L48 Sulphonated, cross-linked polystyrene with an outer layer of submicron, porous, anion-exchange microbeads, 5 to Please enquire 15 µm in diameter L49 A reversed-phase packing made by coating a thin layer of polybutadiene on to spherical porous zirconia particles, 3 Please enquire to 10 µm in diameter Please enquire Multifunction resin with reverse-phase retention and strong anion-exchange functionalities. The resin consists of L50 ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15 µm in diameter, and a surface area of not less than 350 m2/g. Substrate is coated with quartenary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene L51 Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 3 to 10 µm in diameter CHIRALPAK AD, CHIRALPAK AD-H, CHIRALPAK AD-3 L52 A strong cation exchange resin made of porous silica with sulfopropyl groups, 5 to 10 µm in diameter Please enquire Please enquire Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to Please enquire L53 15 µm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Universal Cation, Universal Cation HR Please enquire Capacity not less than 500 µEq/column L54 A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, 5 to 15 µm in diameter L55 A strong cation exchange resin made of porous silica coated with polybutadiene-maleic acid copolymer, about 5 µm in diameter L56 Propyl silane chemically bonded to totally or superficially porous silica particles, 3 to 10 µm in diameter 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 23 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC USP Chromatographic Columns Listing Description Our Brand L57 A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 µm in diameter, with a pore Please enquire size of 120 Å NUCLEOGEL Sugar Na, Shodex CXpak P-421S, Shodex RSpak L58 Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the DC-613, Shodex SUGAR KS-801, Shodex SUGAR KS-802, Shodex sodium form, about 6 to 30 µm diameter SUGAR KS-803, Shodex SUGAR KS-804, Shodex SUGAR KS-805, Shodex SUGAR KS-806, Shodex SUGAR KS-807 L59 Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 Shodex KW400 series, Shodex PROTEIN KW-800 series kDa. The packing is spherical 1.5 to 10 µm, silica or hybrid packing with a hydrophilic coating L60 Spherical, porous silica gel, 10 µm or less in diameter, the surface of which has been covalently modified with alkyl ACME Amide/C18, HALO RP-Amide, Nucleodur PolarTec, amide groups and endcapped Nucleosil C18 Nautilus A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 13 µm microporous L61 particles having a pore size less than 10 units and consisting of ethylvinylbenzene cross-linked with 55% divinylben- Please enquire zene with a latex coating composed of 85 nm diameter microbeads bonded with alkanol quarternary ammonium ions (6%) L62 C30 silane bonded phase on a fully porous spherical silica, 3 to 15 µm in diameter Chromegabond C30 L63 Glycopeptide teicoplanin linked through multiple covalent bonds to a 100 Å spherical silica Please inquire L64 Strongly basic anion exchange resin consisting of 8% crosslinked styrene divinylbenzene copolymer with a Please enquire quartenary ammonium group in the chloride form, 45 to 180 µm in diameter Please enquire L65 Strongly acidic cation exchange resin consisting of 2% sulfonated crosslinked styrene divinylbenzene copolymer with a sulfonic acid group in the hydrogen form, 63 to 250 µm in diameter L66 A crown ether coated on a 5 µm particle size silica gel substrate. The active site is (S)-18-crown-6-ether CROWNPAKTM CR (+) L67 Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer, 2 to 10 µm in Shodex Asahipak ODP-40, Shodex Asahipak ODP-50, diameter Shodex ET-RP1 L68 Spherical, porous silica, 10 µm or less in diameter, the surface of which has been covalently modified with alkyl InertSustain Amide, Inertsil Amide amide groups and not endcapped L69 Ethylvinylbenzene/divinylbenzene substrate agglomerated with quaternary amine functionalized 130 nm latex Please enquire beads, about 6.5 µm in diameter L70 Cellulose tris(phenyl carbamate) coated on 5 µm silica CHIRALCEL OC-H 24 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide USP Chromatographic Columns Listing Description Our Brand L71 A rigid, spherical polymetacrylate, 4 to 6 µm in diameter Shodex RSpak DE-213, Shodex RSpak DE-413, HPLC/UHPLC Column Selection Guide Shodex RSpak DE-613 L72 (S)-phenylglycine and 3,5-dinitroaniline urea linkage covalently bonded to silica Please enquire L73 A rigid, spherical polydivinylbenzene particle, 5 to 10 µm in diameter Jordi Resolve DVB Please enquire A strong anion-exchange resin consisting of a highly cross-linked core of 7 µm macroporous particles having a Resolvosil BSA L74 100 Å average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion- Please enquire Please enquire exchange layer grafted to the surface, which is functionalized with alkyl quartenary ammonium ions Primesep D, Primesep SB, Primesep B2 L75 A chiral-recognition protein, bovine serum albumin (BSA), chemically bonded to silica particles, about 7 µm in CHIRALPAK AS-H diameter, with a pore size of 300 Å L76 Silica based weak cation-exchange material, 5 µm in diameter. Substrate is surface polymerized polybutadiene- maleic acid to provide carboxylic acid functionalities. Capacity not less than 29 µEq/column Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 6 to L77 9 µm diameter. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 500 µEq/column (4 mm x 25 cm) A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and anion-exchange (primary, L78 secondary, or tertiary amino groups) functional groups chemically bonded to porous or non-porous or ceramic micro-particles, 1 to 50 µm in diameter or a monolithic rod L79 A chiral-recognition protein, human serum albumin (HSA), chemically bonded to silica particles, about 5 µm in diameter L80 Cellulose tris(4-methylbenzoate)-coated, porous, spherical, silica particles, 5 to 20 µm in diameter CHIRALCEL OJ, CHIRALCEL OJ-H, CHIRALCEL OJ-3 A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 9 µm porous particles L81 having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex Please enquire coating composed of 70 nm diameter microbeads (6% crosslinked) bonded with alkanol quartenary ammonium ions L82 Polyamine chemically bonded to cross-linked polyvinyl alcohol polymer, 4 to 5 µm in diameter Shodex Asahipak NH2P-40, Shodex Asahipak NH2P-50 A hydroxide-selective, strong anion-exchange resin-quartenary amine bonded on latex particles attached to a core Please enquire L83 of 10.5 µm microporous particles having a pore size of 10 Å and consisting of ethylvinylbenzene cross-linked with Please enquire 55% divinylbenzene. Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 5 µm L84 diameter. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 8400 µEq/column (5 mm x 25 cm) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 25 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC USP Chromatographic Columns Listing Description Our Brand L85 A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and weak cation-exchange Primesep 100, Primesep 200, Primesep C, Primesep 500 (carboxyl groups) functional groups chemically bonded to porous or non-porous particles, 1 to 50 µm in diameter L86 A 5 µm fused core particle with a highly polar ligand possessing 5 hydroxyl groups tethered to the silica gel Halo Penta-HILIC outer layer L87 Dodecyl silane chemically bonded to porous silica particles, 1.5 to 10 µm in diameter Please enquire L88 Glycopeptide vancomycin linked through multiple covalent bonds to 100 Å spherical silica Please enquire Packing having the capacity to separate compounds with a molecular weight range from 100 – 3000 Da (as deter- Please enquire L89 mined by polyethylene oxide), applied to neutral and anionic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylate ether (surface contains some residual cationic functional groups) L90 Amylose tris-[(S)-alpha-methylbenzylcarbamate] coated on porous, spherical silica particles, 3 to 10 µm in diameter CHIRALPAK AS, CHIRALPAK AS-H, CHIRALPAK AS-3 L91 Strong anion-exchange resin consisting of monodisperse porous polystyrene/divinyl benzene beads coupled with Please enquire quartenary amine. Bead size is 3 to 10 µm Please enquire CHIRACEL OD-RH, CHIRACEL OD-3R A strong anion-exchange resin consisting of a highly cross-linked core of 5 – 9 µm macroporous particles having Please enquire L92 a 100 Å average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion Halo Penta-HILIC InertSustain AQ-C18 exchange layer grafted to the surface, which is functionalized with alkanol quartenary ammonium ions Please enquire Please enquire L93 Cellulose tris(3,5-dimethylphenylcarbamate) reversed phase chiral stationary phase coated on 3 or 5 µm silica gel particles L94 A strong anion-exchange resin consisting of a highly crosslinked 15 µm microporous particles functionalized with very low crosslinked latex (0.5%) to provide alkanol quartenary ammonium ion exchange sites L95 Highly polar alkyl ligand comprising five hydroxyl groups that are chemically bonded to totally porous or superficially porous silica or a monolithic silica rod Alkyl chain, reversed-phase bonded to totally porous or superficially porous silica designed to retain hydrophilic L96 and other polar compounds when using highly aqueous mobile phases, including 100% aqueous, 1.5 µm to 10 µm in diameter Weak cation-exchange resin consisting of a highly cross-linked core of 5.5 µm porous particles having a pore size of L97 2000 Å units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 2400 µEq/column (4 mm x 25 cm) Weak cation-exchange resin consisting of a highly cross-linked core of 8 µm microporous particles having an aver- L98 age pore size of 10 Å units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 46 µEq/column (4 mm x 5 cm) 26 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide USP Chromatographic Columns Listing Description Our Brand L99 Amylose tris-(3,5-dumethylphenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in CHIRALPAK IA, CHIRALPAK IA-3 HPLC/UHPLC Column Selection Guide diameter Please enquire A 55% crosslinked, microporous, hydrophobic resin core (9 µm microporous particles having a pore size of 10 Å) Cosmocore Cholester, Cosmosil Cholester L100 that consists of a bilayer of anion and cation exchange latex. The first layer is fully sulfonated (140 nm) and the (S,S) Whelk-O 1 Please enquire second layer is fully aminated (76 nm) Please enquire Please enquire L101 Cholesteryl groups chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in Please enquire diameter, or a monolithic rod CHIRALCEL OJ-RH L102 (Naproxen, (S,S)Whelk-O 1) 1-(3,5-dinitrobenzamido)-1,2,3,4-tetrahydrophenanthrene covalently bonded to porous CHIRALPAK CBH spherical silica particles, 5 to 10 µm in diameter Please enquire A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 7.5 μm porous Please enquire L103 particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene Please enquire Please enquire electrostatically bonded with hyperbranched alkanol quaternary ammonium ions L104 Triazole groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter L105 A strong anion-exchange resin consisting of a highly cross-linked 9 μm super macroporous (2000 Å) particles functionalized with very low cross-linked latex (0.2%) to provide alkyl quaternary ammonium ion sites Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, L106 5 to 8 μm diameter, macroporous particles having an average pore size of 100 Å. Substrate is surface grafted with carboxylic acid and phosphonic acid functional groups. Capacity not less than 2800 μEq/column (4 mm x 25 cm) L107 Cellulose tris(4-methylbenzoate)-coated porous spherical particles, 3 to 5 μm in diameter, for use with reversed-phase mobile phases L108 A chiral-recognition protein, cellobiohydrolase (CBH), chemically bonded to silica particles, about 5 μm in diameter L109 Spherical particles of porous graphitic carbon, 3 to 30 μm in diameter L110 A strong anion-exchange resin consisting of a highly cross-linked 13 μm microporous (less than 10 Å) particles coated with very low cross-linked latex (0.5%) to provide alkanol quaternary ammonium ion exchange sites L111 Polyamine chemically bonded to porous spherical silica particles, 5 μm in diameter A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 8.5 μm porous L112 particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (5% cross-linked) bonded with alkanol quaternary ammonium ions 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 27 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC USP Chromatographic Columns Listing Description Our Brand L113 A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 7.5 μm porous Please enquire particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene SeQuant ZIC-HILIC with a latex coating composed of 65 nm diameter microbeads (8% cross-linked) bonded with alkanol quaternary Please enquire ammonium ions Please enquire CROWNPAK CR(-) L114 Sulfobetaine graft-polymerized to totally or superficially porous silica, 1.5 to 10 μm in diameter, or a monolithic rod. ACME™ PAH, NUCLEODUR C18 PAH Packing having densely bonded zwitterionic groups with 1:1 charge balance. CHIRALPAK IC, CHIRALPAK IC-3` Please enquire L115 Ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with quaternary amine functionalized Please enquire 275 nm latex microbeads (6% cross-linked), about 8.5 µm in diameter CHIRALCEL OD-R Please enquire L116 Sulfonated ethylvinylbenzene/divinylbenzene substrate agglomerated with hydrophilic quaternary amine COSMOSIL PYE functionalized glycidyl-derivative methacrylate microbeads, approximately 2 to 50 μm in diameter CHIRALPAK IC, CHIRALPAK IC-3 Please enquire L117 A crown ether coated on a 5 μm particle size silica gel substrate. The active site is (R)-18-crown-6-ether L118 Aqueous polymerized C18 groups on silica particles, 1.2 to 5 μm in diameter L119 Cellulose tris-(3,5-dichlorophenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 13 µm microporous L120 particles having a pore size of less than 10 Å units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (8% crosslinked) bonded with alkanol quaternary ammonium ions. Capacity not less than 10 mEq/column (4 mm x 5 cm) L## Ethylhexyl triazone, FluoFix – Fluorocarbon chains chemically bonded to 5 μm spherical silica particles L## Lanatoprost, Chiracel OD-R – Cellulose tris(3,5-dimethylphenylcarbamate) coated on 10 μm silica gel particles Polyethylene Glycol 3350, Aquagel OH 40 – Packing having the capacity to separate compounds with a molecular L## weight range from 10,000 to 200,000 g/mol (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers, composed of a rigid macroporous material with a hydrophilic surface L## Felodipine Extended-release Tablets, COSMOSIL PYE – Pyrenyl groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter, or a monolithic rod L## Atomoxetine Hydrochloride, Chiralpak IC – Cellulose tris-(3,5-dichlorophenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 μm in diameter Sodium Nitrite, IonPac AS12A – A strong anion-exchange resin consisting of a highly cross-linked 9 μm L## supermacroporous (2000 Å) particles functionalized with very low cross-linked latex (0.2%) to provide alkyl quartenary ammonium ion sites 28 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide USP Chromatographic Columns Listing Description Our Brand L## Liquid Glucose, Aminex HPX-42A – Strong cation-exchange resin consisting of sulfonated cross-linked Please enquire HPLC/UHPLC Column Selection Guide styrene-divinylbenzene copolymer in the silver form, average 9 μm in diameter CHIRALCEL OJ-RH L## Rosuvastatin Calcium, Chiracel OJ-RH – Cellulose tris(4-methylbenzoate)-coated porous spherical particles, Please enquire 3 to 5 μm in diameter, for use with reversed phase mobile phases Cosmosil HILIC Please enquire L## Palonosetron Hydrochloride, Chirobiotic-V – Glycopeptide vancomycin linked through multiple covalent bonds Please enquire to 100 Å spherical silica Please enquire Please enquire L## Allantoin, Cosmosil HILIC – Triazol groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter Please enquire EPTACOG alfa, Dionex CarboPac PA100 – Ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) L## agglomerated with quartenary amine functionalized 275 nm latex microbeads (6% cross-linked), about 8.5 μm in diameter EPTACOG alfa, Dionex DNAPac PA100 – Sulfonated ethylvinylbenzene/divinylbenzene substrate approximately L## 12 to 14 μm in diameter agglomerated with hydrophilic quartenary amine functionalized glycidyl-derivative methacrylate microbeads Adenosine, Dionex IonPac CS12A and CG12A - Weak cation-exchange resin consisting of ethylvinylbenzene, L## 55% cross-linked with divinylbenzene copolymer, 5 to 8 μm diameter, macroporous particles having an average pore size of 100 Å. Substrate is surface grafted with carboxylic acid and phosphonic acid functional groups. Capacity not less than 2800 μEq/column (4 mm x 25 cm) Adenosine, Dionex IonPac AG18 - A hydroxide-selective, strong anion-exchange resin consisting of a highly L## crosslinked core of 13 μm microporous particles having a pore size of <10 Å and consisting of ethylvinylbenzene crosslinked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (8% crosslinked) bonded with alkanol quartenary ammonium ions. Capacity not less than 10 μEq/column (4 mm x 5 cm) Adenosine, Dionex IonPac AS18 - A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 7.5 μm macroporous particles having an average pore size of 2000 Å and consisting of L## ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (8% crosslinked) bonded with alkanol quartenary ammonium ions. Capacity not less than 285 μEq/column (4 mm x 25 cm) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 29 LIFE SCIENCE

Genesis Genesis HPLC columns were developed by Jones Chromatography. Key Features Hichrom acquired this range of columns from Grace. Genesis phases are based on high purity, metal-free, spherical silica. They are suitable for the Good peak shape and reproducibility analysis of a wide range of compounds. Long column lifetime Genesis Phase Specifications pH stability 1 to 10 Phase Particle size / µm Endcapped? Properties USP code C18 3, 4, 5 Yes Excellent peak symmetry. Reduced need for mobile phase modifiers. L1 C8 4, 5 No Long column life. L7 C8(EC) 4 Yes Suitable for lower pH separations. L7 Excellent peak symmetry. Reduced need for mobile phase modifiers. AQ 4 Yes Long column life. L1 Designed for separating hydrophilic and polar compounds. Stable retention Phenyl 4, 5 Yes times in 100 % aqueous mobile phases. Rapid equilibration. L11 Cyano 4 Yes Unique reversed-phase selectivity. L10 Silica 4 − Reversed-phase chemistry. Improves the chromatography of polar aromatics, L3 fatty acids, and basic pharmaceuticals. Suitable for polar analysis, analytes with double- and/or triple-bonds, and compounds that have too much retention on alkyl phases. Highly polar phase for general purpose applications. Ordering Information Analytical Columns 3 µm Length / mm 50 100 150 250 i.d. / mm 3.0 4.6 4.0 4.6 2.1 4.6 3.0 4.6 C18 FL5963E FM5963E FH10963E FM10963E FK15963E FM15963E FL25963E FM25963E 4 µm Length / mm 30 50 100 125 i.d. / mm 3.0 3.0 4.0 4.6 C18 2.1 3.0 2.1 FL5960E 4.6 2.1 FL10960E 4.6 GEN-4C18-125DF GEN-4C18-125D C8 FK5960E − FM5960E FK10960E FL10962E FM10960E −− C8(EC) FK3960E – FK5962E − − FM10962E −− AQ FK5964E − − − − FM10964E −− Cyano – FL3962E − FM5964 − − FM10951E −− − FM5951E − FM10965E1 −− − − − −− −− 1Also available in PEEK, metal-free column hardware (FM10965EP). Length / mm 150 250 Guard cartridges (3/pk)1 i.d. / mm 4.0 4.6 3.0 4.0 4.6 For 3.0-4.6 C18 FH15960E FM15960E FL25960E FH25960E FM25960E 5169650/N C8 − FM15962E − − FM25962E FH1962-2/N C8(EC) − FM15964E − FH25964E FM25964E − AQ − FM15951E − − FM25951E − Experts in Chromatography Cyano − FM15965E2 − − FM25965E − Phenyl − FM15980E − − FM25980E − Silica − − − − FM25961E FH1961-2/N 1To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 2Also available with reversed-phase shipping solvent (FM15965ER). 5 µm Length / mm 125 150 250 I.d. / mm 4.6 4.6 4.6 C18 GEN-5C18-125D GEN-5C18-150D GEN-5C18-250D C8 − − GEN-5C8-250D Phenyl − − GEN-5PH-250D Semi-preparative and Preparative Columns 4 µm Length / mm 150 250 I.d. / mm 10 10 C18 − 8P25960 Silica 8P15961 − 12 For free, impartial, no obligation technical advice and support, contact [email protected]

Column: Genesis C18, 4 μm, 100 x 4.6 mm (p/n: FM10960E) Genesis continued Mobile phase: A cetonitrile:50 mM KH2PO4, pH 3.0 (40:60) Column: Genesis CN, 4 μm, 150 x 4.6 mm (p/n: FM15965E) Flow rate: 1.0 mL min−1 Mobile phase: Acetonitrile:0.05 % Trichloroacetic Acid in Water, pH 3.0 Flow rate: 1.0 mL min−1 Detector: UV at 254 nm Detector: UV at 254 nm 1. Atazine Desisopropyl 1. Atenolol 2. Atrazine Desethyl 2. Metoprolol 3. Simazine 3. Propranolol 4. Propazine 4. Diltiazem 1 14 2 3 3 2 4 0 1 2 3 4 5 0 2 4 6 8 10 Figure 4. Anti-Anginal Drugs Mins Figure 5. Atrazine Herbicides Mins +44 (0)118 930 3660 | [email protected] | www.hichrom.com 13

Prevail The Prevail range of HPLC columns were developed by Alltech. Hichrom Key Features acquired this range from Grace. The Prevail range exhibits long lifetimes in both highly aqueous and highly organic mobile phases. The stability Stable from highly organic to highly aqueous of these phases is such that a single column can be switched between highly aqueous, for analysis of highly polar analytes, and highly organic, Speciality phases for specific applications for strong retention of hydrophobic analytes. Excellent sensitivity with microbore and ELSD applications Prevail Phase Specifications Phase Particle size / µm Properties Applications USP code C18 Flexibility to switch between varied mobile phase L1 3, 5 Stable in highly aqueous to highly organic mobile conditions to suit a variety of applications. Excellent C18-Select phases sensitivity for microbore applications L1 C8 Suitable for applications where greater retention than L7 3, 5 Stable in highly aqueous to highly organic mobile the Prevail C18 is required phases Use for highly hydrophobic compounds that retain too L11 strongly on C18 5 Stable C8 phase L10 L8 Phenyl 5 Lowest hydrophobic capacity Selective for aromatic compounds in a variety of mobile − phase conditions L3 − Cyano 3, 5 General purpose cyano suitable for normal or reversed- Rugged normal phase applications phase use Amino Amide 3, 5 Stable in highly aqueous to highly organic mobile Use for carbohydrates or as a weak anion exchanger Silica phases Organic acid 5 Polar embedded phase Suitable for use with highly aqueous mobile phases 3, 5 Highly polar phase General purpose normal phase applications 3, 5 Highly efficient silica-based, acid-stable phase Separates common organic acids with unsurpassed resolution, speed and sensitivity. Lower cost than polymeric columns Prevail Organic Acid Prevail Organic Acid columns are silica-based for maximum efficiency and high resolution. They can separate common organic acids with a combination of speed, sensitivity, and simplicity. A simple acidic phosphate buffer and a Prevail OA column at ambient temperature will separate 11 short chain organic acids in less than 6 minutes. The retention profile can be readily adjusted by changing the mobile phase pH. Prevail Carbohydrate ES The Prevail Carbohydrate ES column was discontinued by Grace prior to the acquisition of the Prevail range by Hichrom. Please contact our technical team on +44 (0)118 930 3660 or [email protected] to discuss suitable alternatives for your application. Ordering Information Experts in Chromatography Analytical Columns 3 µm Length / mm 20 50 100 150 Guard cartridges (3/pk)1 i.d. / mm 2.1 3.0 For 2.1 C18 43827 2.1 4.6 2.1 4.6 2.1 99322 4.6 96683/N C18-Select − 99200 99315 99204 96691/N Cyano − 43818 43829 43871 99202 99313 − 99303 − Amino − 99243 − 99247 − Silica − 99309 − 99312 99302 99255 99341 99259 − Organic acid − − 99271 − −− −− − 88655 − −− − 99257 −− 43805 − −− 88648 − 1To be used with All-Guard cartridge holder (80101/N) and column coupler (HI-081). 88755 – Prevail Organic Acid, 3 µm, 150 x 4.6 mm (compatible with Waters instruments) 14 For free, impartial, no obligation technical advice and support, contact [email protected]

Prevail continued 3 µm – Rocket Columns For column dimensions not listed here, Rocket columns are specifically designed for high-throughput and please contact high-speed methods. +44 (0)118 930 3660 or Length / mm 33 53 [email protected] i.d. / mm 7 7 C18 99280 99279 to discuss your requirements C18-Select 99304 99305 Organic Acid − 50755 5 µm Length / mm 50 100 125 2.1 150 4.6 250 4.6 Guard cartridges (3/pk)1 Experts in Chromatography i.d. / mm 4.6 4.6 4.6 99206 3.0 99208 3.0 99210 For 2.1 For 3.0-4.6 C18 43903 − PRE-5C18-125D 99320 99300 99321 99301 96682/N 99286/N C18-Select − − − − 99316 99224 99317 99229 96690/N 96455/N C8 − − − 99218 − 99239 5131428 99241 99128/N 99287/N Phenyl − − − 99237 99326 99251 99327 99253 99130/N 99288/N Cyano − − − 99329 99263 − 99265 99131/N 99353/N Amino − − − − − 88660 − 88665 99135/N 99290/N Amide − PRE-5AM-100D − 99261 − 99275 − 99277 −− Silica − − − 99339 88640 99340 88645 99133/N 99354/N Organic acid − − − − − PRE-5OA-250DT − 96429/N 99273 − 1To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 5 μm – Columns Compatible with Waters Instruments Length / mm 150 250 Guard cartridges (3/pk)1 i.d. / mm 4.6 4.6 For 3.0-4.6 C18 99209 99211 99286/N Organic Acid 88740 88745 96429/N 1To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (Waters type column port: HI-881). Semi-preparative and Preparative Columns 5 µm Length / mm 150 250 i.d. / mm 22 C18 99296 10 22 99294 99297 +44 (0)118 930 3660 | [email protected] | www.hichrom.com 15

Ion Chromatography Allsep Anion The Allsep Anion column range was developed by Alltech. Hichrom Key Features acquired this column range from Grace. Allsep Anion is a methacrylate- based phase with quaternary ammonium functional groups, optimised 7 µm polymer-based anion exchange phase for use with both suppressed and non-suppressed conductivity Suppressed or non-suppressed detection. Columns are compatible with common IC mobile phases, such as carbonate, bicarbonate, p-hydroxybenzoic acid, phthalic acid, conductivity detection succinic acid, and sodium octane sulfonate. pH range 2–10 Use with 0–100 % organic modifier Allsep Anion is recommended for applications involving inorganic USP L23 anions, weak and strong acid ions, metal complexes and organic acids. It meets the requirements for the EPA method 300.0 Part A for determination of inorganic ions in water. Ordering Information 7 µm Length / mm 50 100 2.1 150 250 i.d. / mm 4.6 4.6 51210 4.6 2.1 Stainless Steel 51214 51200 51212 PEEK (metal-free) 51213 51207 − 51208 − 51209 Length / mm Guard cartridges Guard cartridges Guard cartridge Guard cartridge (3/pk)1 (3/pk)1 kits2 kits2 i.d. / mm For 2.1 For 2.1 Stainless Steel For 3.0–4.6 For 3.0–4.6 PEEK (metal-free) 38110/S 38111/S 38108/S 38109/S − − 38108 38109 1All-Guard cartridge holder and coupler (80101/N and HI-081) for stainless steel hardware and 80101 for PEEK hardware required. 2Includes All-Guard cartridge holder and coupler (80101/N and HI-081) for stainless steel hardware, 80101 for PEEK hardware, and 3 guard cartridges. Hichrom Organic Acid Experts in Chromatography Hichrom Organic Acid columns are ion exclusion columns packed with Key Features sulphonated polystyrene-divinylbenzene. The OA-1000 and OA-2000 columns exhibit excellent selectivity for aliphatic and aromatic acids. Rapid analysis of organic acids and alcohols As with most ion exclusion columns, a column heater in necessary for pH stable polymer resin normal operating procedures. Isocratic 100 % aqueous mobile phases The IOA-1000 and IOA-2000 columns are suitable for the separation only - no organic solvents of citric and other acids from glucose and fructose. USP L17 Ordering Information Part number Phase Particle size / µm Column dimensions / mm Applications 9046 OA-1000 9 300 x 6.5 Inorganic ions, such as fluoride, arsenate, sulphite, alcohols and most organic acids 9048 OA-2000 6.5 100 x 6.5 Organic acids with low pH values, low MW straight chain acids and 9646 IOA-1000 9 300 x 7.8 aromatic acids 9648 IOA-2000 8 150 x 6.5 Acids of the tricarboxylic acid cycle (Krebs cycle) Fast separation of acids and some alcohols 28884 – Guard cartridges for Hichrom Organic Acid columns (2/pk). 28883 – Guard cartridge kit for Hichrom Organic Acid columns, including guard cartridge holder and 2 guard cartridges. 16 For free, impartial, no obligation technical advice and support, contact [email protected]

Ion Chromatography continued Hichrom Anion Exclusion Hichrom Anion Exclusion columns are based on a highly sulphonated Key Features polystyrene-divinylbenzene cation-exchange resin. The phase has a particle size of 10 μm and is designed for the separation of organic Separates organic acids and weakly acids and weakly ionised anions by an anion exclusion mechanism. ionised anions Typical mobile phases contain dilute mineral acids. Acetonitrile (<10%) may be added as organic modifier to decrease the retention of Polymer-based for broad pH stability hydrophobic compounds. USP L22 Ordering Information Key Features 10 µm Silica-based for symmetrical peak shapes Separates inorganic and organic anions Length / mm 100 300 i.d. / mm 7.8 7.8 Anion Exclusion (stainless steel) 269068 269006 Hichrom Anion/S Hichrom Anion/S columns are based on 10 µm silica with quaternary ammonium ion-exchange groups. These columns are optimised for non-suppressed conductivity detection and are best suited for routine separations of chloride, bromide, nitrate, and sulphate. Ordering Information 10 µm Length / mm 100 250 i.d. / mm 4.6 4.6 Stainless Steel 269013 269001 PEEK (metal-free) 269012 269011 Hichrom Carbohydrate Cation The Hichrom Carbohydrate Cation column consists of a highly efficient Key Features sulphonated polystyrene resin supplied in the calcium form. This column provides excellent separations using only water as the mobile phase. Sulphonated polystyrene resin (Ca2+ form) Ordering Information 100% water used as mobile phase Column heating required 70057 – Hichrom Carbohydrate Cation, 10 µm, 300 x 6.5 mm USP L19 Experts in Chromatography Length / mm 300 i.d. / mm 6.5 Hichrom Carbohydrate Cation 70057 Hichrom Pre-saturation Columns All silica HPLC packing materials will gradually dissolve in aqueous solution. This problem is exacerbated when the pH level of the solution is above 7. To avoid silica dissolution, mobile phases should ideally be buffered between pH 3.5–6.5. Where high levels of aqueous solvent or alkaline pH conditions are required, the pre-saturation column (packed with large 40 µm particle diameter silica) saturates the mobile phase with silica to minimise analytical column dissolution problems. Ordering Information 28610 – Hichrom pre-saturation column, 250 x 4.6 mm (1/pk) 28650 – Hichrom pre-saturation column, 250 x 4.6 mm (4/pk) +44 (0)118 930 3660 | [email protected] | www.hichrom.com 17

Vydac Vydac 300 Å columns are a commonly employed range in bioseparations. Following the acquisition of Grace HPLC ranges by Hichrom, Vydac columns continue to be available exclusively from Hichrom in capillary to microbore and analytical to preparative dimensions. Separate biomolecules from small peptides to large intact proteins with the Vydac family of reversed-phase columns (including Vydac TP, MS, Denali, and Everest) and ion exchange (Vydac 302IC) columns. Reversed-phase columns for a polypeptide separation should be considered on the basis of the polypeptide’s hydrophobicity, with molecular weight as a secondary consideration. Vydac TP Vydac TP reversed-phase material consists of aliphatic groups bonded Key Features to the surface of 300 Å pore diameter silica. The large pores of the TP silica give polypeptide molecules complete access to the interior of the Long column lifetime and negligible silica pores. Vydac TP silica is the standard that has defined large pore phase leaching HPLC for polypeptide separations for nearly two decades. Reliable protein purifications, scalable Vydac TP Phase Specifications from analytical to preparative scale Referenced in a large number of patents and publications Phase Functional group Particle size / µm Properties Applications USP code 218TP C18 3, 5, 10 First generation polymeric C18 phase Small polypeptides 4-5 kDa MW, enzymatic digest L1 with unique selectivity fragments, natural and synthetic peptides, multi- 238TP C18 5 ring compounds L1 201TP C18 5, 10 First generation monomeric C18 phase Use for same applications as 218TP, but offers L1 202TP C18 different C18 selectivity L1 208TP C8 5 Non-endcapped C18 phase L7 214TP C4 3, 5, 10 Higher carbon load than 201TP Developed for separation of PAHs L26 214ATP C4 5, 10 Less hydrophobic than C18TP phase L26 Developed for separation of PAHs 219TP Diphenyl 5 First generation C4 phase L11 C4 phase with lower level of Polypeptides 10-20 kDa MW 5, 10 endcapping Glycoproteins, haemoglobin variants, histones, Lowest capacity first generation insulin variants, membrane proteins diphenyl phase Optimised for analysis of human growth hormone Polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides, fusion proteins from inclusion bodies Ordering Information Capillary Columns 5 μm Experts in Chromatography Length / mm 150 i.d. / mm 218TP 0.3 0.5 214TP 218TP5.315 − 214TP5.315 214TP5.515 Analytical Columns 3 μm Length / mm 50 100 i.d. / mm 3.0 218TP 2.1 218TP3305 4.6 3.0 4.6 208TP 218TP3205 − 218TP3405 208TP3405 − 218TP3410 − 208TP3310 208TP3410 Length / mm 150 250 i.d. / mm 218TP 2.1 4.6 3.0 4.6 208TP 218TP3215 218TP3415 − 218TP34 −− 208TP33 − 18 For free, impartial, no obligation technical advice and support, contact [email protected]

Length / mm Guard cartridges (2/pk)1 Guard cartridge kits2 Vydac TP continued i.d. / mm For 2.1 For 3.0–4.6 For 2.1 For 3.0–4.6 218TP 218GD32/N 218GD34/N 218GK32/N 218GK34/N 208TP − 208GD34/N − 208GK34/N 1 To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 2 Includes holder (80101/N), column coupler (HI-081), and 1 guard cartridge. 5 μm Length / mm 30 50 i.d. / mm 2.1 218TP − 1.0 2.1 3.0 4.6 238TP − 218TP5105 218TP5405 201TP 201TP5203 238TP5105 218TP5205 218TP5305 238TP5405 208TP − 201TP5105 201TP5405 214TP − 208TP5105 238TP5205 − 208TP5405 214ATP − 214TP5105 214TP5405 219TP − 201TP5205 − 214ATP5405 − 219TP5405 219TP5105 208TP5205 − 214TP5205 214TP5305 −− 219TP5205 − Length / mm 1.0 100 3.0 4.6 i.d. / mm 218TP5110 2.1 − 218TP5410 218TP 238TP5110 218TP5210 − 238TP5410 238TP − 201TP5310 201TP5410 201TP 208TP5110 201TP5210 − 208TP5410 208TP 214TP5110 208TP5210 214TP5310 214TP5410 214TP 214TP5210 − 214ATP − 214ATP5210 − − 219TP − 219TP5210 219TP5410 Length / mm 1.0 150 3.0 4.6 i.d. / mm 218TP5115 2.1 − 218TP5415 218TP 238TP5115 218TP5215 238TP5315 238TP5415 238TP 201TP5115 238TP5215 201TP5315 201TP5415 201TP 201TP5215 − 202TP5415 202TP − − 208TP5315 208TP5415 208TP 208TP5115 208TP5215 214TP5315 214TP5415 214TP 214TP5115 214TP5215 − 214ATP5415 214ATP 214ATP5215 219TP5315 219TP5415 219TP − 219TP5215 219TP5115 4.0 Length / mm 2.1 250 − 4.6 i.d. / mm 1.0 218TP52 3.0 − 218TP54 218TP 218TP51 238TP52 218TP53 201TP540 238TP54 238TP 238TP51 201TP52 238TP53 − 201TP54 201TP 201TP51 − − 202TP54 202TP − − − 208TP54 208TP − 208TP52 208TP53 − 214TP54 214TP 208TP51 214TP52 214TP53 − 214ATP54 214ATP 214TP51 214ATP52 − 219TP54 219TP 219TP52 219TP53 − 219TP51 Length / mm Guard cartridges (2/pk)1 Guard cartridge kits2 i.d. / mm For 1.0 For 2.1 For 3.0-4.6 For 1.0 For 2.1 For 3.0–4.6 218TP 218GD51/N 218GD52/N 218GD54/N 218GK51/N 218GK52/N 218GK54/N 238TP 238GD51/N 238GD52/N 238GD54/N 238GK51/N 238GK52/N 238GK54/N Experts in Chromatography 201TP − 201GD52/N 201GD54/N − 201GK52/N 201GK54/N 202TP − − 202GD54/N − − 202GK54/N 208TP 208GD51/N 208GD52/N 208GD54/N 208GK51/N 208GK52/N 208GK54/N 214TP 214GD51/N 214GD52/N 214GD54/N 214GK51/N 214GK52/N 214GK54/N 214ATP − − 214AGD54/N − − 214AGK54/N 219TP − 219GD52/N 219GD54/N − 219GK52/N 219GK54/N 1 To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 2 Includes holder (80101/N), column coupler (HI-081), and 1 guard cartridge. 10 μm Length / mm 50 150 1.0 250 4.6 Guard cartridges (2/pk)1 Guard cartridge kits2 i.d. / mm 4.6 4.6 218TP101 2.1 218TP104 For 3.0-4.6 For 3.0-4.6 218TP − 218TP10415 218TP102 201TP104 218GD104/N 218GK104/N 201TP − 201TP10415 − − 208TP104 201GD104/N 201GK104/N 208TP 208TP10405 208TP10415 − − 214TP104 208GD104/N 208GK104/N 214TP − 214TP10415 214TP101 − 214GD104/N 214GK104/N 1 To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 19 2 Includes holder (80101/N), column coupler (HI-081), and 1 guard cartridge. +44 (0)118 930 3660 | [email protected] | www.hichrom.com

Vydac TP continued 10–15 µm 150 250 4.6 4.6 Length / mm 5119751 218TP10154 i.d. / mm − 214TP10154 218TP − 214TP101015 214TP 214TP 15–20 µm Length / mm 50 100 150 250 Guard cartridges (2/pk)1 Guard cartridge kits2 i.d. / mm 4.6 4.6 4.6 4.6 For 3.0-4.6 For 3.0-4.6 218TP 218TP1520405 218TP1520410 218TP1520415 − − − 208TP − 208TP1520410 − − − − 214TP − − − 214TP15204 214GD15204/N 214GK15204/N 1To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 2 Includes holder (80101/N), column coupler (HI-081), and 1 guard cartridge. 20–30 µm Length / mm 150 i.d. / mm 4.6 218TP 218TP2030415 Semi-preparative and Preparative Columns 5 µm Length / mm 100 150 250 Guard cartridges (2/pk)1 Guard cartridge kits2 i.d. / mm 10 10 For 10–22 For 10–22 218TP 10 22 218TP51015 218TP510 218GCC510 218FSK510 238TP − 238TP510 − − 201TP 218TP51010 − − 201TP510 − − 208TP − 208TP510 208GCC510 208FSK510 214TP −− 214TP51015 214TP510 214GCC510 214FSK510 214ATP − 214ATP510 − − 219TP −− − 219TP510 219GCC510 219FSK510 208TP51010 − 214TP51010 214TP52210 −− −− 1To be used with semi-prep guard holder (GCH10) and column coupler for All-Guard cartridges (HI-081). 2Includes holder (GCH10), column coupler (HI-081), and 1 guard cartridge. 10 µm Length / mm 100 150 250 Guard cartridges (2/pk)1 i.d. / mm 22 For 10–22 218TP 218TP102210 10 22 10 22 218GCC1010 208TP − 208GCC1010 214TP − 218TP101015 218TP102215 218TP1010 218TP1022 214GCC1010 219TP − 219GCC1010 −− 208TP1010 208TP1022 214TP101015 214TP102215 214TP1010 214TP1022 −− 219TP1010 219TP1022 1To be used with semi-prep guard holder (GCH10) and column coupler for All-Guard cartridges (HI-081). 10–15 µm Experts in Chromatography Length / mm 150 250 Guard cartridges – 12 µm (2/pk)1 Guard cartridge kits – 12 µm2 i.d. / mm 10 22 10 22 For 10–22 For 10–22 218TP 218TP101510 218TP101522 218GCC1210 218FSK1210 208TP 214TP10151015 214TP10152215 208TP101510 208TP101522 208GCC1210 208FSK1210 214TP 214TP101510 214TP101522 214GCC1210 214FSK1210 1To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 2Includes holder (80101/N), column coupler (HI-081), and 1 guard cartridge. 15–20 µm Length / mm 100 150 250 Guard cartridges (2/pk)1 i.d. / mm 22 22 For 10–22 218TP 218TP15202210 218TP15202215 10 22 − 208TP − − − 214TP − − 218TP152010 218TP152022 214GCC152010 −− 214TP152010 214TP152022 1To be used with semi-prep guard holder (GCH10) and column coupler for All-Guard cartridges (HI-081). 20 For free, impartial, no obligation technical advice and support, contact [email protected]

Vydac MS Vydac MS is a further development of the Vydac range for reversed- Key Features phase separation of biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns unique selectivity. A variety 300 Å pore size spherical silica of reversed-phases makes this product line suitable for the analysis of Four reversed-phase chemistries small peptides to large intact proteins. Excellent peak shape with little or no TFA High protein recoveries make scale-up easy Vydac MS Phase Specifications Phase Functional Particle size Properties Applications USP code 218MS group / µm L1 238MS C18 5, 10 Polymeric bonding, highest hydrophobic interaction Use for simple enzymatic digests (<12 proteins) or L1 208MS L7 214MS C18 5 and unique geometric selectivity biomolecules 0–5 kDa MW L26 C8 5, 10 Monomeric bonding offers increased peptide Use for same applications as 218MS, but offers C4 5, 10 interaction and generally yields higher peak counts different C18 selectivity Lower hydrophobicity is better for larger biomolecules Ideal for biomolecules 5–10 kDa MW Lower capacity than C18 or C8, suitable for Ideal for biomolecules >10 kDa MW, intact proteins, hydrophobic proteins or when minimal organic antibodies, oligonucleotides, human growth hormone solvent is desired Ordering Information Capillary Columns 5 µm Length / mm 0.075 50 0.3 0.5 i.d. / mm 218MS5.07505 0.15 218MS5.305 218MS5.505 218MS 238MS5.07505 218MS5.1505 238MS5.305 238MS5.505 238MS 208MS5.07505 238MS5.1505 208MS5.305 208MS5.505 208MS 214MS5.07505 208MS5.1505 214MS5.305 214MS5.505 214MS 214MS5.1505 0.3 Length / mm 0.075 0.1 100 218MS5.310 0.5 i.d. / mm 218MS5.07510 − 0.15 238MS5.310 218MS5.510 218MS 238MS5.07510 − 218MS5.1510 208MS5.310 238MS5.510 238MS 208MS5.07510 208MS5.1010 238MS5.1510 214MS5.310 208MS5.510 208MS 214MS5.07510 − 208MS5.1510 214MS5.510 214MS 214MS5.1510 0.5 0.075 218MS5.515 Length / mm 218MS5.07515 150 238MS5.515 i.d. / mm 238MS5.07515 208MS5.515 218MS 208MS5.07515 0.15 0.3 214MS5.515 Experts in Chromatography 238MS 214MS5.07515 208MS 218MS5.1515 218MS5.315 0.5 214MS 218MS5.525 238MS5.1515 238MS5.315 238MS5.525 208MS5.525 208MS5.1515 208MS5.315 214MS5.525 214MS5.1515 214MS5.315 Length / mm 250 i.d. / mm 218MS 0.075 0.15 0.3 238MS 218MS5.07525 208MS 238MS5.07525 218MS5.1525 218MS5.325 214MS 208MS5.07525 214MS5.07525 238MS5.1525 238MS5.325 208MS5.1525 208MS5.325 214MS5.1525 214MS5.325 +44 (0)118 930 3660 | [email protected] | www.hichrom.com 21

Vydac MS continued Analytical Columns 5 µm Length / mm 20 50 i.d. / mm 2.1 218MS 2.1 4.6 1.0 218MS5205 4.6 238MS 218MS5105 238MS5205 218MS5405 208MS 218MS5202 218MS5402 238MS5105 208MS5205 214MS 208MS5105 214MS5205 − −− 214MS5105 − 4.6 214MS5405 −− 218MS5410 238MS5410 200 −− 1.0 − 218MS5120 Length / mm 100 214MS5410 − i.d. / mm − 218MS 1.0 2.1 3.0 4.6 − 238MS 218MS5110 218MS5415 208MS 238MS5110 218MS5210 218MS5310 238MS5415 214MS 208MS5110 208MS5415 214MS5110 238MS5210 − 214MS5415 208MS5210 − 4.6 218MS54 214MS5210 214MS5310 238MS54 208MS54 Length / mm 150 214MS54 i.d. / mm 218MS 1.0 2.1 3.0 238MS 218MS5115 208MS 238MS5115 218MS5215 − 214MS 208MS5115 214MS5115 238MS5215 − 208MS5215 208MS5315 214MS5215 214MS5315 Length / mm 250 i.d. / mm 218MS 1.0 2.1 3.0 238MS 218MS51 208MS 238MS51 218MS52 218MS53 214MS 208MS51 214MS51 238MS52 − 208MS52 − 214MS52 214MS53 Length / mm Guard cartridges (2/pk)1 Guard cartridge kits2 i.d. / mm 218MS For 1.0 For 2.1 For 3.0-4.6 For 1.0 For 2.1 For 3.0-4.6 238MS 208MS 218GD51MS/N 218GD52MS/N 218GD54MS/N 218GK51MS/N 218GK52MS/N 218GK54MS/N 214MS 238GD51MS/N 238GD52MS/N 238GD54MS/N 238GK51MS/N 238GK52MS/N 238GK54MS/N 208GD51MS/N 208GD52MS/N 208GD54MS/N 208GK51MS/N 208GK52MS/N 208GK54MS/N 214GD51MS/N 214GD52MS/N 214GD54MS/N 214GK51MS/N 214GK52MS/N 214GK54MS/N 1To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 2Includes holder (80101/N), column coupler (HI-081), and 1 guard cartridge. 10 µm Length / mm 250 Guard cartridges (2/pk)1 Guard cartridge kits2 i.d. / mm For 3.0-4.6 For 3.0-4.6 Experts in Chromatography 218MS 1.0 4.6 208MS 218GD104MS/N 218GK104MS/N 214MS 218MS101 218MS104 − − − − − 208MS104 − 214MS104 1To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 2Includes holder (80101/N), column coupler (HI-081), and 1 guard cartridge. 10–15 µm 250 4.6 Length / mm 214MS10154 i.d. / mm 214MS 22 For free, impartial, no obligation technical advice and support, contact [email protected]

Vydac MS continued Semi-preparative and Preparative Columns For column dimensions not listed here, 5 µm please contact Length / mm 250 i.d. / mm 10 +44 (0)118 930 3660 or 218MS 218MS510 208MS 208MS510 [email protected] 214MS 214MS510 to discuss your requirements 10 µm 150 250 Experts in Chromatography 22 Length / mm 218MS102215 10 22 i.d. / mm 208MS102215 218MS − 218MS1010 218MS1022 208MS 214MS − 208MS1022 214MS1010 214MS1022 10–15 µm Length / mm 250 Guard cartridges – 12 µm (2/pk)1 i.d. / mm 22 For 22 214MS 214MS101522 214FSK1210MS 1To be used with semi-prep guard holder (GCH10) and column coupler for All-Guard cartridges (HI-081). +44 (0)118 930 3663 | [email protected] | www.hichrom.com 23

Vydac Everest Everest columns (238EV) have unique selectivity and sensitivity, Key Features which are the result of bonding technology that improves C18 surface coverage and deactivates residual silanols. Leading 300 Å C18 Unique selectivity for hydrophilic and chemistries have had carbon coverage in the 2.8 to 3.6 µmol m−2 range. hydrophobic peptides Everest C18 coverage is in excess of 4 µmol m−2 and approximates the theoretical limit based on surface area. The increased shielding of the 300 Å pore size spherical silica base silica increases column lifetime and reduces the amount of TFA required to shield the silica. Excellent sensitivity with little or no TFA in mobile phase Ordering Information Ideal for complex enzymatic digests (>12 proteins) Capillary Columns 5 µm Length / mm 0.075 50 0.5 0.075 100 0.3 i.d. / mm 238EV5.07505 0.3 238EV5.505 238EV5.07510 0.15 238EV5.310 C18 238EV5.305 238EV5.1510 0.5 Length / mm 150 238EV5.515 250 i.d. / mm C18 0.075 0.15 0.3 0.15 0.3 238EV5.07515 238EV5.1515 238EV5.315 238EV5.1525 238EV5.325 Analytical Columns 5 µm Length / mm 50 100 150 i.d. / mm 2.1 C18 1.0 4.6 2.1 3.0 1.0 238EV5215 4.6 238EV5115 238EV5415 238EV5105 238EV5405 238EV5210 238EV5310 Length / mm 250 Guard cartridges (2/pk)1 Guard cartridge kits2 i.d. / mm 2.1 For 3.0-4.6 C18 1.0 238EV52 4.6 For 2.1 For 3.0-4.6 238GK54EV/N 238EV51 238EV54 238GD52EV/N 238GD54EV/N 1To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 2Includes holder (80101/N), column coupler (HI-081), and 1 guard cartridge. Semi-preparative Columns 5 µm 10 µm Length / mm 250 Guard cartridges (2/pk)1 Length / mm 250 i.d. / mm 10 For 10-22 i.d. / mm 22 C18 238EV510 C18 238EV1022 238GCC510EV 1To be used with semi-prep guard holder (GCH10) and column coupler for All-Guard cartridges (HI-081). Experts in Chromatography Column: Vydac Denali C18, 5 μm, 150 x 4.6 mm (p/n: 218DE5415) 9 1. Phenol 2. 4-Nitrophenol Mobile phase: A : 1 % Acetic Acid in Water 3. 2-Chlorophenol B:11 % Acetic Acid in Acetonitrile 4. 2-Nitrophenol 5. 2,4-Dinitrophenol Gradient: Time / min 0 25 3 26 27 28 6. 2,4-Dimethylphenol 7. 4-Chloro-3-Methylphenol 1. Atenolol % A 07 49 30 30 70 8. 2,4-Dichlorophenol 2. Metoprolol 9. 2-Methyl-4,6-Dinitrophenol %B 30 51 70 70 30 10. 2,4,6-Trichlorophenol 2 2 11. Pentachlorophenol F3l.oPwropraratneo: l1olmL min−1 4 10 11 D4.eDteilctitaozre:mUV at 280 nm 57 1 3 68 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Figure 6. EPA 604 Phenols Mixture 24 For free, impartial, no obligation technical advice and support, contact [email protected]

Vydac Denali Key Features Vydac Denali (238DE) is a 120 Å C18 bonded phase with high High retentiveness carbon coverage, suitable for the analysis of both acidic and basic LC/MS of small molecules analytes. It has applications for small molecule analyses of interest to Fully scalable from capillary to process pharmaceutical and environmental laboratories. Ordering Information Capillary Columns 5 µm 0.075 50 0.3 0.5 238DE5.07505 0.15 238DE5.305 238DE5.505 Length / mm 238DE5.1505 i.d. / mm 0.5 C18 238DE5.515 Length / mm 100 0.075 150 250 i.d. / mm 0.075 238DE5.07515 0.15 0.3 C18 238DE5.07510 238DE5.1515 238DE5.325 Analytical Columns 5 µm Length / mm 50 100 i.d. / mm 2.1 3.0 C18 1.0 238DE5205 238DE5305 4.6 1.0 2.1 3.0 4.6 238DE5105 238DE5405 238DE5110 238DE5410 238DE5210 238DE5310 4.6 Length / mm 150 250 238DE54 i.d. / mm 2.1 C18 1.0 2.1 3.0 4.6 1.0 238DE52 238DE5115 238DE5415 238DE51 238DE5215 238DE5315 Length / mm Guard cartridges (2/pk)1 Guard cartridge kits2 i.d. / mm C18 For 1.0 For 2.1 For 3.0-4.6 For 1.0 For 2.1 For 3.0-4.6 238GD51DE/N 238GD52DE/N 238GD54DE/N 238GK51DE/N 238GK52DE/N 238GK54DE/N 1To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). 2Includes holder (80101/N), column coupler (HI-081), and 1 guard cartridge. 10 µm Length / mm 50 150 250 4.6 300 i.d. / mm 4.6 4.6 4.0 238DE104 4.6 C18 238DE10405 238DE10415 DE-10C18-250DF 5174066 DE-10C18-300DF Silica − − – – Semi-preparative and Preparative Columns 5 µm Length / mm 100 10 150 250 Experts in Chromatography i.d. / mm 10 5174053 22 10 C18 238DE51010 5174054 238DE510 Silica − 238DE52215 − − 10 µm Length / mm 150 250 Guard cartridges (2/pk)1 i.d. / mm 10 22 For 10-22 C18 238DE101015 238DE102215 10 22 Silica − 5174057 238GCC1010DE 238DE1010 238DE1022 − 5174063 − 1To be used with semi-prep guard holder (GCH10) and column coupler (HI-081). +44 (0)118 930 3660 | [email protected] | www.hichrom.com 25

Vydac 302IC Anion-Exchange Vydac 302IC is a low capacity anion-exchange (quaternary amine) Key Features material based on a high purity 10 µm large pore size silica. Well referenced for non-suppressed ion Ordering Information chromatography applications Used with standard HPLC systems for analysis of common ions Widely used for environmental analysis Analytical Columns 10 µm Length / mm 100 250 Guard cartridges (2/pk)1 i.d. / mm 4.6 For 4.6 302IC 302IC4610 2.1 4.6 302GD4/N 302IC102 302IC4.6 1To be used with All-Guard cartridge holder (80101/N) and column coupler for All-Guard cartridges (HI-081). Experts in Chromatography 26 For free, impartial, no obligation technical advice and support, contact [email protected]

Guard Cartridge Systems To support these newly acquired column ranges, Hichrom continues to Benefits of the hardware recommend the use of guard cartridges for analytical column protection improvement include: and increased column lifetime. The hardware of the Hichrom All-Guard Cartridge System has been improved and all guard cartridges and holders Higher pressure rating are now supplied in this new hardware, leading to more robust and higher efficiency separations. Chromatographic selectivity will be unaffected by Low internal swept volume this change. Please note that the new Hichrom guard cartridges and holders are not compatible with the old design Alltech guard cartridges and holders. Guard cartridges and holders supplied in the new improved hardware are denoted by the addition of an ‘N' at the end of the old part number. HPLC Column Guard Cartridge Holder (p/n 80101/N) Column Coupler Guard Cartridge for Guard Holder (p/n HI-081) Ordering information 80101/N – All-Guard cartridge holder HI-081 – Column Coupler for All-Guard Cartridge Holder (standard column port) HI-881 – Column Coupler for All-Guard Cartridge Holder (Waters type column port only) C-1000 – Semi-prep guard holder (for 10-22 mm i.d. columns) GCH10 – Vydac semi-prep guard holder (for 10-22 mm i.d. columns) HI-226 – Analytical Guard Holder Wrench Set - contains a pair of 9/16” stainless steel wrenches HI-050X – Hichrom PEEK Fingertight Fittings (10/pack) - suitable for connection of HPLC columns and guard holders to all 1/16” tubing types, are compatible with both standard and Waters port designs, and are slip free to >5,000 psi Please contact Hichrom for other former Grace brands not featured in this catalogue, including: Experts in Chromatography • Adsorbosil • Brava • Exsil • Grom Sapphire • ProSphere • Adsorbosphere • Econosil • GraceSmart • Macrosphere • VisionHT • Allsphere • Econosphere • Grom Sil • Platinum This catalogue contains HPLC column ranges acquired by Hichrom from Grace. For information on any other product ranges within the Hichrom portfolio, please contact [email protected] or [email protected] Alternatively, call us on: +44 (0)118 930 3660 +44 (0)118 930 3660 | [email protected] | www.hichrom.com 27

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