CS Lab Essentials

diagnostics Microbiology QC1™ Quality Control Slides QC1 AFB Slides Overview: QC1™ AFB Quality Control Slides eliminate the need to maintain control cultures or to utilize positive samples, allowing labs to easily standardize their stain quality validation procedures. They offer consistent, reliable results – along with ease of use – for accurate stain quality validation. Key Features • Offer consistent, reliable results along with ease of use for accurate stain quality validation. • Eliminate need to maintain control cultures or retain positive results • Individually packaged with positive and negative controls • Compatible with Kinyoun, Ziehl-Neelsen, and Fluorescent staining procedures • Contain [+] control of Mycobacterium scrofulaceum and [-] control of Escherichia coli www.calibrescientific.com 101

diagnostics Microbiology QC1™ Quality Control Slides QC1 Gram Slides Overview: QC1 Gram Slides are intended to be used to validate each batch of slides stained by the conventional Gram stain technique. They can be used to objectively assess the performance of equipment, reagents, methods and techniques, while eliminating the need to maintain in-house cultures of control organisms. Slides are available in standard format (with only positive and negative controls) or with either one or three wells for patient specimens — making them ideal for the second and third shifts for supervisor review. Key Features • Three configurations to choose from • Individually packed in sealed foil pouches to preserving the positive control Staphylococcus aureus, and negative control Escherichia coli, until you are ready to use them • Quality Control Log sheets are available for download Direct Order Link 102 www.calibrescientific.com

diagnostics Microbiology QC1™ Quality Control Slides QC1 Gram Slides ORDERING INFORMATION Product Name Product Code Product Description Quality 10 slides/pk ATS-0003233 (GG&B Stainer) 40 slides/pk One specimen well 40 slides/pk ATS-0003230 Three specimen wells 40 slides/pk 10 slides/pk QC1™ Gram Slides ATS-0003238 40 slides/pk ATS-0003231 ATS-0003236 ATS-0003235 www.calibrescientific.com 103

diagnostics Microbiology CELL-BOND® Slides Overview: Our CELL-BOND® Adhesive Slides contains a unique non-albumin-based, highly wettable, bio-adhesive coating that allows for rapid sample bonding without the use of heat treatment, methanol fixation, or external adhesives. They decrease competitive background staining, leading to increased microscopic clarity. Adopting CELL-BOND Slides in your lab can allow for easier, more efficient microscopic diagnostics. Key Features • Rapid specimen bonding • Decreased competitive background staining • Reduced altered organism and tissue morphology • Non-albumin-based adhesive • Custom printed configurations available (based upon order quantity). Direct Order Link ORDERING INFORMATION Product Name Product Code Product Description Quantity 100 slides/pk ATS-0003217 1 specimen well, 12 mm round, (black background) CELL-BOND ATS-0003219 Adhesive Slides ATS-0003223 1 specimen well, 12 mm round, (blue background) ATS-0003224 1 red specimen well, 15 mm round, (no background) 1 specimen well, 15 mm round, (black background) 104 www.calibrescientific.com

diagnostics Microbiology NAC-PAC System NAC-PAC RED Overview: NAC-PAC® is a decontamination and digestion system for the recovery of Mycobacterium spp. The included N-acetyl-L-cysteine (NALC) supports mucolytic activity for up to 72 hours. NAC PAC® RED is used in the Digestion and Decontamination procedure of clinical specimens and provides confirmation of neutral pH without titration. Key Features • Provides visual confirmation of a neutral pH without titration • Creates the ideal final sample characteristics for mycobacterial testing methods • Earlier growth detection in automated systems • Compatible with instrumented and manual mycobacterial growth systems, and molecular methods • Reduced reprocessing from breakthrough contamination and false-positive results Direct Order Link ORDERING INFORMATION NOTE: Each NAC-PAC RED package contains (5) NAC-PAC RED bottles, and (5) pre-measured NALC ampules (unless otherwise noted) Product Name Product Code Product Description Quantity ATS-0003441 5 x 50 ml 2.0% NaOH ATS-0003457 5 X 200 ml 5 x 50 ml 2.5% NaOH 5 X 200 ml NAC-PAC RED 1 X 200 ml 2.5% NaOH (no NALC ampules) 5 x 50 ml 3.0% NaOH 5 X 200 ml 1 X 200 ml 3.0% NaOH (no NALC ampules) 5 x 50 ml 4.0% NaOH 5 X 200 ml www.calibrescientific.com 105

diagnostics Sanger Sequencing SupreDye v3.1 Cycle Sequencing Kit Overview: As the proven alternative to BigDye® Terminator Cycle Sequencing Kits, the SupreDye™ Cycle Sequencing Kits provide all the sequencing-required components in a ready, pre- mixed format. Both single-stranded or double-stranded DNA templates from PCR and plasmids can be sequenced. v3.1 is for longer reading such as de novo sequencing and resequencing purpose. Key Features • Proven high-quality performance • Uniform peak heights and long, high-resolution reads • Increased robustness for difficult to sequence templates • Direct, Drop-in Substitute! o No change necessary in protocol, system settings, or calibrations. o Fully compatible with all ABI genetic analyzers Kit Components • SupreDye reaction ready sequencing premix • pGEM control DNA • M13 control primer • 5x sequencing buffer Direct Order Link ORDERING INFORMATION Product Name Unit Size Product Code SupreDye™ v3.1 Cycle Sequencing Kit 24 EDG-063200 SupreDye™ v3.1 Cycle Sequencing Kit 100 EDG-063002 SupreDye™ v3.1 Cycle Sequencing Kit EDG-063008 SupreDye™ v3.1 Cycle Sequencing Kit 1,000 EDG-063020 SupreDye™ v3.1 Cycle Sequencing Kit 2,500 EDG-063040 5,000 106 www.calibrescientific.com

diagnostics Sanger Sequencing Optima™ DTR (Sanger Clean-Up Kit) Optima™ DTR 96-well plate kit. Optima™ DTR Ultra 96-well plate kit. Overview: Optima™ DTR 96 well plate Kits are used in cleaning up Sanger sequencing reactions. The gel filtration plates consist of columns in standardised array and provide optimised performance for the removal of unincorporated BigDye v1.1, v3.0 and v3.1 and other dye terminators, dNTP’s, salts and other molecular weight materials from sequencing reactions. Key Features • Generate long contiguous sequence read lengths (>800 bases) with a low retest rate (average CRL is 900 or more bases) • Utilize a rapid, simple protocol with minimal hands-on time that allows you to process more samples for sequencing every day (only 3 steps and less than 15 minutes to complete) • Provide a cost-effective solution for high throughput applications and are ideal for use with robotic pipettors • Available in a wide range of formats: Single sample, 8-well, 96-well short and Ultra, and 384-well formats (Ultra plates contain a greater quantity of resin a taller column height and provide even higher sensitivity results than with short plates) • Does not require low temperature (4°C) storage Direct Order Link ORDERING INFORMATION Product Name Product Code Column Size Quantty Purifications 440uL 2 plates 192 Optima DTR™ EDG-45637 800uL 10 plates 960 96-Well Plate Kit EDG-17946 2 plates 192 10 plates 960 Optima DTR™ Ultra EDG-45891 96-Well Plate Kits EDG-17832 www.calibrescientific.com 107

diagnostics Sanger Sequencing Performa® DTR Gel Filtration Cartridges Overview: erforma® DTR is our original dye terminator removal product for clean-up of Sanger sequencing reactions and is available in single sample spin cartridges, and 96-well formats. Key Features • Generate long contiguous sequence read lengths (>800 bases) with a low retest rate (average CRL is 900 or more bases) • Utilize a rapid, simple protocol with minimal hands-on time that allows you to process more samples for sequencing every day (only 3 steps and less than 15 minutes to complete) • Provide a cost-effective solution for high throughput applications and are ideal for use with robotic pipettors • Are available in a wide range of formats: Single sample, 8-well, 96-well short and Ultra, and 384-well formats (Ultra plates contain a greater quantity of resin a taller column height and provide even higher sensitivity results than with short plates) • Do not require low temperature (4°C) storage (can be stored at ambient temperature for up to 4 weeks without affecting performance) Direct Order Link ORDERING INFORMATION Product Code Unit Size Product Name EDG-98780 108/pk Performa® DTR Gel Filtration Cartridges 108 www.calibrescientific.com

diagnostics Sanger Sequencing Adhesive Plate Sealers Overview: Adhesive Plate Sealers are ideal for protecting samples in 96- or 384-well plates during shaking, incubation, and storage at -20°C, 4°C, or ambient temperature. They are packaged in sets of 25 sealers, can be secured to any flat surface to eliminate waste and clutter, and are resistant to heat and cold (-20°C to 100°C). Key Features • Secure, leak-proof seal with no special tools required • Removes cleanly with no adhesive residue • Will not tear when removed, even when stored at -20°C • Designed for easy, rapid application Direct Order Link ORDERING INFORMATION Product Code Unit Size Product Name EDG-48461 100 Adhesive Plate Sealers www.calibrescientific.com 109

diagnostics Buffers & Assay Kits MagBead L6 Viral RNA Lysis Buffer Overview: MagBead L6 Viral RNA Lysis Buffer is intended for the isolation and purification of total nucleic acids (DNA/RNA) from biological specimens for in vitro diagnostic procedures. The L6 buffer is recommended by the COVID-19 Consortium, UK and has been manufactured according to their SOP Methods. Key Features MagBead L6 Viral RNA Lysis Buffer is designed for: • Nasopharyngeal swab samples • Lysis of DTT pre-treated sputum samples which had been spun down to pellets before adding the RNA Lysis buffer • Stabilization of nucleic acids with lysates • Nucleic acid purification using magnetic beads • Chemical inactivation of wet and dry swabs for up to 4 weeks without affecting performance) Direct Order Link ORDERING INFORMATION Product Code Unit Size Product Name BSV-COV-003 125mL BSV-COV-L6-005 500mL 2X MagBead Viral RNA Lysis Buffer BSV-COV-L6-006 10L MagBead L6 Viral RNA Lysis Buffer MagBead L6 Viral RNA Lysis Buffer 110 www.calibrescientific.com

diagnostics Sanger Sequencing Adhesive Plate Sealers Overview: Adhesive Plate Sealers are ideal for protecting samples in 96- or 384-well plates during shaking, incubation, and storage at -20°C, 4°C, or ambient temperature. They are packaged in sets of 25 sealers, can be secured to any flat surface to eliminate waste and clutter, and are resistant to heat and cold (-20°C to 100°C). Key Features • Secure, leak-proof seal with no special tools required • Removes cleanly with no adhesive residue • Will not tear when removed, even when stored at -20°C • Designed for easy, rapid application Direct Order Link ORDERING INFORMATION Product Code Unit Size Product Name EDG-48461 100 Adhesive Plate Sealers www.calibrescientific.com 111

diagnostics Buffers & Assay Kits MagBead L6 Viral RNA Lysis Buffer Overview: MagBead L6 Viral RNA Lysis Buffer is intended for the isolation and purification of total nucleic acids (DNA/RNA) from biological specimens for in vitro diagnostic procedures. The L6 buffer is recommended by the COVID-19 Consortium, UK and has been manufactured according to their SOP Methods. Key Features MagBead L6 Viral RNA Lysis Buffer is designed for: • Nasopharyngeal swab samples • Lysis of DTT pre-treated sputum samples which had been spun down to pellets before adding the RNA Lysis buffer • Stabilization of nucleic acids with lysates • Nucleic acid purification using magnetic beads • Chemical inactivation of wet and dry swabs for up to 4 weeks without affecting performance) Direct Order Link ORDERING INFORMATION Product Code Unit Size Product Name BSV-COV-003 125mL BSV-COV-L6-005 500mL 2X MagBead Viral RNA Lysis Buffer BSV-COV-L6-006 10L MagBead L6 Viral RNA Lysis Buffer MagBead L6 Viral RNA Lysis Buffer 112 www.calibrescientific.com

diagnostics Buffers & Assay Kits Sorensen’s Buffers Sorensen’s Buffer pH6.8 | Sorensen’s Buffer pH6.9 Sorensen’s Buffer pH7.0 | Sorensen’s Buffer pH7.2 Overview: Sorensen’s Phosphate Buffer is used in the differential staining of blood films and pathological specimens where rinsing in “buffered water” or “low salt buffer” is stipulated in the protocol. It is supplied at a 10 fold concentration (0.5g/L) solution of sodium phosphate that can be made within a pH range of pH 5 to pH 8 by adjusting the proportions of monobasic and dibasic phosphate salts. Key Features • Can be used as “buffered water” in a variety of Romanovsky staining procedures • Available in a range of different pH’s Direct Order Link ORDERING INFORMATION Description Product Code Unit Size Product Name 610210 5 x 25 mL Supplied in concentrated liquid 625080 5 x 25 mL Sorensen’s Buffer pH6.8 form. Each pack of 5 x 25 mL 610450 5 x 25 mL Sorensen’s Buffer pH6.9 and will make a total of 25 Litres 610460 5 x 25 mL Sorensen’s Buffer pH7.0 working solution. Dilute 5mL to 1 Sorensen’s Buffer pH7.2 Litre for use. www.calibrescientific.com 113

diagnostics Buffers & Assay Kits Shephards Stain Kit (Modified Kleihauer) Overview: Shepard’s Stain Kit (Modified Kleihauer) is used for screening and quantification of feto-maternal haemorrhage (FMH) in a maternal blood smear. It works on the principle that Foetal haemoglobin (Hb F) is less soluble in an acidic environment than adult haemoglobin (Hb A) and the kit exploits this difference. Key Features • Can be performed at room temperature and does not require heat as in the older technique. • RBC’s containing Hb F stain brilliantly with eosin, while those without Hb F appear as colorless “ghosts”. • The hematoxylin stains the nuclei of the WBC’s blue to prevent misinterpretation as eosin-positive cells. Direct Order Link Kit Components • Shephards Counterstain • Shephards Stain 1 (Alcoholic Haematoxylin) • Shephards Stain 2 (Acidified Ferric Chloride) ORDERING INFORMATION Product Code Quantity Product Name 610920 Counterstain (1L) Shephards Complete Stain Solution 1 (500mL) (Modified Kleihauer) Solution 2 (250mL) 114 www.calibrescientific.com

diagnostics Buffers & Assay Kits Sickle Test Kit Overview: The Sickle-Test is a haemoglobin solubility screening test to detect haemoglobin S (Hb-S) found in patients with sickle cell anaemia and sickle cell trait. It can be used to demonstrate the presence of Haemoglobin S (Hb-S) in an EDTA-anticoagulated blood sample by the haemoglobin solubility test method. Key Features • The kits are offered in a range of sizes to suit your workload with sickle-cell controls in a separate pack. • The working solution of test reagent is stable for up to one month if kept refrigerated Direct Order Link Kit Components • Reagent A (Buffered Saponin) • Reagent B (Sodium Dithionite) • Viewing Card ORDERING INFORMATION Description Product Code Number of Tests Product Name 5 bottles of Reagent A 610830-LU 25 Sickle Test (25 test Kit) 5 bottles of Reagent B Sickle Test (50 test Kit) (different pack sizes depending 610680-LU 50 Sickle Test (100 test Kit) on the number of tests) 610850-LU 100 1 viewing card www.calibrescientific.com 115

diagnostics Buffers & Assay Kits Measles IgM Capture ELISA Overview: An enzyme Immunoassay (EIA) for the detection of human IgM antibodies to measles virus in serum, plasma and oral fluid. This product is for in-vitro diagnostic use by trained laboratory personnel. Testing of oral fluid samples is recommended for surveillance of measles. Positive IgM results should be confirmed with other tests, such as PCR on well timed specimens. Key Features • The kit uses oral fluid which makes collection of sample simple, cheap and non-invasive. • Reduces the risk of exposure to HIV and hepatitis associated with use of needles. Direct Order Link Kit Components • Anti-human IgM plates • Serum diluent • Wash buffer • Positive Control ORDERING INFORMATION Description Product Code Number of Tests Product Name Anti-human IgM plates (8x12) MeVM010 Tests 92 Measles IgM Capture EIA Serum diluent (100mL) specimens Wash buffer (100mL) Positive Control (1.4mL) 116 www.calibrescientific.com

diagnostics Buffers & Assay Kits Mumps IgM Capture EIA Overview: An enzyme immunoassay (EIA or ELISA) for the detection of mumps virus specific IgM in human oral fluid, serum and plasma samples Key Features • The kit uses oral fluid which makes collection of sample simple, cheap and non- invasive. • Reduces the risk of exposure to HIV and hepatitis associated with use of needles. Direct Order Link Kit Components • Anti-human IgM plates • Serum diluent • Wash buffer • Positive Control ORDERING INFORMATION Description Product Code Number of Tests Product Name Anti-human IgM plates (8x12) MuVM004-LU Tests 92 Mumps IgM Capture EIA Serum diluent (100mL) specimens Wash buffer (100mL) Positive Control (1.4mL) www.calibrescientific.com 117

chemical essentials Chemical Essentials Cell Culture Description: Application: ORDERING INFORMATION For determining best media Product Code: formulation of recombinant proteins expressed in E. coli MDL-MD12-185 Media Optimization Kit Expression of recombinant PAL-GEN-S-02122-5G Ultrapure IPTG (> 99.5%) proteins in E. Coli PAL-GEN-S-02122-25G Ultrapure IPTG (> 99.5%) Culturing a wide range PAL-IPTG-200-5 1M IPTG Solution of cell types PAL-IPTG-1000-5 1M IPTG Solution PAL-IPTG-200-100 1M IPTG Solution PAL-IPTG-1000-100 1M IPTG Solution MD12-410 DMEM/F12 1:1 Serum-Free MD12-411 IMDM - Serum-Free Media Used for rapid proliferating and high-density cell cultures  MD12-402 BRFF-EPM2 Optimized for culturing human oesophageal epithelial cell lines. MD12-401 BRFF-BMZERO™ Optimized for culturing human breast cells in a serum free media 118 www.calibrescientific.com

chemical essentials Chemical Essentials Plant Science ORDERING INFORMATION Description: Application: Product Code: Plant tissue culture growth PTL-M524-1L Murashige and Skoog Basal Salt medium Mixture Used as a solution for tissue P4001 PhytoAx™ culture to promote the growth PTL-A111-100G Agar, Micropropagation Grade of axillary shoots PTL-A1375-10L Arabidopsis Germination Medium Gelling agent for plant tissue culture production Medium containing all the components for Arabidopsis seed germination www.calibrescientific.com 119

chemical essentials Chemical Essentials Standard Lab Buffers ORDERING INFORMATION 100ml 250ml 1L Chemical MD2-011-PH MD2-011-PH-250 MD2-011-PH-1L 1M HEPES MD2-013-PH MD2-013-PH-250 MD2-013-PH-1L 1M MES MD2-100-116 MD2-1000-116 MD2-012-PH MD2-250-116 MD2-012-PH-1L 5M Sodium Chloride MD2-012-PH-4M MD2-012-PH-250 MD2-012-PH-1L-4M 1M Imidazole MD2-100-263 MD2-012-PH-250-4M MD2-1000-263 4M Imidazole MD2-100-263-8M MD2-1000-263-8M 2m Urea MD2-250-263 8M Urea MD2-028-PH MD2-250-263-8M MD2-028-PH-1L MD2-100-126 MD2-1000-126 1.6M Sodium citrate MD2-100-29 MD2-022-03 MD2-1000-29 1M Tris Hydrochloride MD2-100-43 MD2-028-PH-250 MD2-1000-43 4M Ammonium acetate MD2-100-45 MD2-1000-45 3.5M Ammonium chloride MD2-250-126 MD2-250-29 5M Lithium chloride MD2-250-43 2M Lithium sulfate MD2-250-45 5M Magnesium chloride MD2-100-47 MD2-250-47 hexahydrate 1M Bis-Tris MD2-004-PH MD2-004-PH-250 MD2-004-PH-1L 1M Bis-Tris propane MD2-005-PH MD2-005-PH-250 MD2-005-PH-1L 1M Potassium Phosphate MD2-018-PH MD2-018-PH-250 MD2-018-PH-1L 1M Potassium phosphate monobasic MD2-100-164 MD2-250-164 MD2-1000-164 1M Potassium phosphate dibasic trihydrate MD2-1000-163 MD2-250-163 MD2-1000-163 1M Sodium phosphate monobasic monohydrate MD2-100-189 MD2-250-189 MD2-1000-189 100% Glycerol MD2-100-65 MD2-250-65 MD2-1000-65 120 www.calibrescientific.com

chemical essentials Chemical Essentials PCR Description: Application: ORDERING INFORMATION For cleanup of primers Product Code: and oligonucleotides EDG-EnzSAP-500 EnzSAP™ PCR Clean-up Reagent EDG-EnzSAP-2000 VRYS-AG314-2 2X PCR Master Mix Ready to use solution containing all the essential ingredients of a PCR reaction SERVA-3971201 dNTP PCR Mix, solution 10 mM Ready to use dNTP mixture for PCR Reactions ABCL-RK20626-100RXN FirstTaq 2X PCR Master Mix with Ready to use solution for Standard Buffer with Dye PCR Reactions ABCL-RK20603-100RXN HotStart Taq 2X PCR Master Mix Ready-to-use solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl2 www.calibrescientific.com 121

chemical essentials Solvent Essentials Structural Biology Overview: • Overview text • Overview text ORDERING INFORMATION Chemical Acronym Name Product Code Dichloromethane, Meets ACS/BP/USP/EP, 25L CS-DCM-R-25L Dichloromethane DCM Dichloromethane, Meets ACS/BP/USP/EP, 200L CS-DCM-R-200L CS-NMP-200L Dichloromethane DCM N-methylpyrrolidone, Meets EP/BP, 200L CS-DMF-PE-200L N,N-dimethylformamide, Peptide Grade, 200L CS-DMA-PE-200L N-methylpyrrolidone NMP N,N-Dimethylacetamide, Peptide Grade, 200L CS-ACN-PU-200L CS-ACN-PU-ISO N,N-dimethylformamide DMF Acetonitrile, Pure Grade, 200L CS-ACN-UP-200L Acetonitrile, Pure Grade, Isotank CS-ACN-UP-ISO N,N-Dimethylacetamide DMA Acetonitrile, Ultra Pure Grade, 200L CS-THF-UP-25L Acetonitrile, Ultra Pure Grade, Isotank CS-THF-UP-200L Acetonitrile ACN Tetrahydrofuran, Ultra Pure Grade, 25L CS-TFA-PE-200L Tetrahydrofuran, Ultra Pure Grade, 200L CS-TFE-PE-200L Acetonitrile ACN Trifluoracetic acid, Peptide Grade, 200L CS-PIP-PE-25L Trifluroethanol, Peptide Grade, 200L CS-PIP-PE-200L Acetonitrile ACN Piperidine, Peptide Grade, 25L CS-TOL-UP-25L Piperidine, peptide Grade, 200L CS-TOL-UP-200L Acetonitrile ACN Toluene, Ultra Pure Grade, 25L CS-IPA-R-200L Tetrahydrofuran THF Toluene, Meets USP, 200L Isopropyl Alcohol, 99%, Meets ACS/BP/USP/EP, 200L Tetrahydrofuran THF Trifluoracetic acid TFA Trifluroethanol TFE Piperidine PIP Piperidine PIP Toluene Toluene Isopropyl Alcohol, 99% IPA 122 www.calibrescientific.com

chemical essentials Solvent Essentials Structural Biology Overview: • Overview text • Overview text ORDERING INFORMATION Chemical Acronym Name Product Code Acetone, Meets ACS/EP/BP/USP, 25L CS-ACE-R-25L Acetone ACE Acetone, Meets ACS/EP/BP/USP, 200L CS-ACE-R-200L Acetone, Meets ACS/EP/BP/USP, 1000L CS-ACE-R-1000L Acetone ACE Acetone, Meets ACS/EP/BP/USP, Isotank CS-ACE-R-ISO CS-ACE-NF-ISO Acetone ACE Acetone, NF Grade, Isotank CS-GAA-R-25L Glacial Acetic Acid, Meets ACS/BP/USP/EP, 25L CS-GAA-R-205L Acetone ACE Glacial Acetic Acid, Meets ACS/BP/USP/EP, 205L CS-PYR-R-200L CS-PYR-UP-200L Acetone ACE Pyridine, Meets EP/BP, 200L CS-PYR-PE-200L Pyridine, Ultra Pure Grade, 200L Glacial Acetic Acid Pyridine, Peptide Grade, 200L Glacial Acetic Acid Pyridine PYR Pyridine PYR Pyridine PYR www.calibrescientific.com 123

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