CS Lab Essentials

life sciences Protein Purification Super and Fastback Loose Resins Affinity Chromatography Resins Overview: • High-capacity Fastback Protein A, Fastback Protein G, Super Ni-NTA, Super Co-NTA, Super Heparin and Super GST affinity resins • Fastback Q and Fastback S resins for ion exchange chromatography • DTT-compatible Ni-NTA, Co-NTA and Glutathione-NTA Agarose • NoEndo™ Resin for specific endotoxin removal • Gold standard in recombinant protein purification • High binding capacity at the right price Direct Order Link ORDERING INFORMATION Description 1ml 5ml 10ml 25ml 100ml Super Ni-NTA Super-NiNTA1 Super-NiNTA10 Super-NiNTA25 Super-NiNTA100 Agarose Resin Super Co-NTA Super-CoNTA1 Super-CoNTA10 Super-CoNTA25 Super-CoNTA100 Agarose Resin Super Glutathione SuperGlu10A SuperGlu25A SuperGlu100A Agarose Resin Super Heparin Super-Hep-10 Super-Hep-25 Super-Hep100 Agarose Resin Fastback Co Fastback-Co-10 Fastback-Co-25 Fastback-Co-100 IMAC Resin Fastback Ni Fastback-Ni-10 Fastback-Ni-25 Fastback-Ni-100 IMAC Resin Fastback Ni Fastback-Ni-Adv-10 Fastback- Ni-Adv-25 Fastback- Ni-Adv-100 Advance Resin Fastback Fastback-PA-1 Fastback-PA-5 Fastback-PA-25 Fastback-PA-100 Protein A Resin Fastback Protein Fastback-PG-1 Fastback-PG-5 Fastback-PG-25 Fastback-PG-100 G Resin Fastback Q Fastback-Q-10 Fastback-Q-25 Fastback-Q-100 IEX Resin Fastback S Fastback-S-10 Fastback-S-25 Fastback-S-100 IEX Resin www.calibrescientific.com 51

life sciences Protein Purification Empty FPLC Column Overview: • Protein Ark offers a range of empty low pressure chromatography columns, which are compatible with FPLC, HPLC, low pressure pump systems or a syringe • Customers can pack their own resin into these columns. Direct Order Link Maximum pressure 1ml column Specifications Maximum pressure 5ml & 10ml column 5bar (70psi) pH stability Recommended flow rate 3bar (42psi) Chemical compatibility 14-Feb 1ml column housing 0.5-2 ml/min 5ml & 10ml column housing • All common aqueous solutions Bed dimensions of the 1ml column • D enaturants e.g., 8 M Urea, 4 M guanidine Bed dimensions of the 5ml column Bed dimensions of the 10ml column hydrochloride and chaotropic salts. End Adaptors (polypropylene) • Not compatible with buffers containing>20% Ethanol Stop Plug Medical grade polypropylene Acrylic 33 x 6.2 mm 52 x 11.0 mm 104 x 11.0 mm 10-32 UNF female thread (1/16”) 10-32 UNF male thread (1/16”) 52 www.calibrescientific.com

life sciences Protein Purification Empty FPLC Column (continued) Direct Order Link ORDERING INFORMATION Quantity PN: Product GEN-FLIQ1 1 unit (1 column body, 2 End Adaptors, GEN-FLIQ5 Proteus 1 ml FliQ column 2 Stop Plugs) GEN-FLIQ10 GEN-10.32 Proteus 5 ml FliQ column 1 unit (1 column body, 2 End Adaptors, 2 Stop Plugs) Proteus 10 ml FliQ column 1 unit (1 column body, 2 End Adaptors, 2 Stop Plugs) 10.32 packing connector Luer/Thread connector for packing FliQ columns www.calibrescientific.com 53

life sciences Protein Purification HiFliQ FPLC Columns Overview: • Pre-packed 1 ml and 5 ml FPLC columns • Fast and reliable affinity purification • Available with high-capacity Protein A, Protein G, Ni-NTA, Co-NTA, Heparin and GST affinity resins • Available with Q and S IEX resins • For use with FPLC, peristaltic pumps and syringes Direct Order Link ORDERING INFORMATION Description: UOM: PN: Protein A FPLC column Protein A FPLC column 5 x 1ml column PAL-HiFliQ1-PA-5 Protein G FPLC column PAL-HiFliQ5-PA-5 Protein G FPLC column 5 x 5ml column PAL-HiFliQ1-PG-1 Ni-NTA FPLC column PAL-HiFliQ1-PG-5 Ni-NTA FPLC column 1 x 1ml column PAL-HiFliQ1-NiNTA-1 PAL-HiFliQ1-NiNTA-5 5 x 5ml column 1 x 1ml column 5 x 1ml column Other Resins Available: • Protein A • Protein G • Nickel-NTA • Cobalt-NTA • Q-type Ion Exchange • S-type Ion Exchange • Glutathione • Heparin • Custom Purification of rabbit IgG’s from serum on a 1 ml HiFliQ Protein A FPLC Column. (A) chromatogram; (B) electrophoresis; lane 1 - MW marker, lane 2 - Rabbit serum, lane 3 - flowthrough, lane 4 - wash pool, lane 5 - eluted IgG. 54 www.calibrescientific.com

life sciences Protein Purification ProteoSEC Dynamic Columns Overview: • 11/30, 16/60 & 26/60 Size Exclusion Columns for AKTAs • Resin is a co-polymer of Dextrose and agarose for identical SuperdexTM S75 & S200 Performance • Packed with 3-70 kDa and 6-600 kDa High Resolution preparative grade resin Direct Order Link ORDERING INFORMATION Description: Order No: SEC-D-11/30-3-70 ProteoSEC Size Exclusion Column 11/30 3-70 HR AKTA Dynamic Column SEC-D-16/60-3-70 SEC-D-26/60-3-70 ProteoSEC Size Exclusion Column 16/60 3-70 HR AKTA Dynamic Column SEC-D-11/30-6-600 SEC-D-16/60-6-600 ProteoSEC Size Exclusion Column 26/60 3-70 HR AKTA Dynamic Column SEC-D-26/60-6-600 ProteoSEC Size Exclusion Column 11/30 6-600 HR AKTA Dynamic Column ProteoSEC Size Exclusion Column 16/60 6-600 HR AKTA Dynamic Column ProteoSEC Size Exclusion Column 26/60 6-600 HR AKTA Dynamic Column ProteoSEC Dynamic 16/60 6-600 HR ProteoSEC Dynamic 16/60 3-70 HR Flowrate: 1 ml/min; sample loading 0.5 ml; mobile phase: PBS (phosphate buffered saline); A. Model proteins 1: Aprotinin (Mr 6,500); 2: Cytochrome c (Mr 12,300); 3: Beta-Lactoglobulin (Mr 35,000); 4: BSA (Mr 67,000); 5: gamma-Globulin IgG (Mr 158,000); 6: Apoferritin (Mr 440,000); 7: Thyroglobulin (Mr 669,000) B. Model proteins 1: Vit B-12 (Mr 1,200); 2: Aprotinin (Mr 6,500); 3: Cytochrome c (Mr 12,300); 4: Beta-Lactoglobulin (Mr 35,000); 5: BSA (Mr 67,000). www.calibrescientific.com 55

life sciences Protein Purification Proteus X-Spinner 2.5 Centrifugal Concentrator Overview: • Ideal for membrane proteins (Use 100 kDa MWCO), viscous samples, dilute protein solutions, protein fractions post Size Exclusion or IMAC. Features • Membrane Type: Cellulose Triacetate and Polyethersulphone • MWCO: 5 kDa, 10 kDa, 20 kDa, 100 kDa & 300 kDa MWCO • Sample Volume Range: 0.1 – 2.5 ml • Hold-up Volume: 25 µl • Pack Sizes: 24 pc & 96 pc • % Protein Recoveries: >99% • Max g force: 2,500 g (Swing Bucket Rotor, 2,000 g (Fixed Angle Rotor) Direct Order Link ORDERING INFORMATION Description: UOM: PN: 12 pc 24 pc PAL-X-TR-12 TRIAL Pack (Assorted MWCOs) 96 pc PAL-X-5-24 Proteus X-spinner 2.5 Pack (5 kDa MWCO) PAL-X-5-96 Proteus X-spinner 2.5 Pack (5 kDa MWCO) % Protein recoveries are improved with the Proteus X-spinner 2.5 56 www.calibrescientific.com

life sciences Electrophoresis Elite Agarose Tablets DNA Electrophoresis Overview: • Tablets contain everything for an agarose gel in desired percentage: pre-weighed agarose, Magnite Green DNA stain (2-in-1, 3-in-1), and buffer (3-in-1). • Enhanced gel reproducibility due to no weighing required. • Ultrapure agarose: high resolution of DNA fragments and low background • Magnite Green DNA stain: safer, non-carcinogenic alternative to Ethidium Bromide • Powder agarose available as well! Direct Order Link ORDERING INFORMATION Description: UOM: PN: Elite Agarose tablets 100 tablets Elite 2-in-1 Agarose tablets 100 tablets PAL-E-AG-100 Elite 3-in-1 TBE Agarose tablets 100 tablets PAL-E-2in1-100 Elite 3-in-1 TAE Agarose tablets 100 tablets PAL-E-3in1-TBE Agarose, low EEO, molecular biology PAL-E-3in1-TAE Agarose, low EEO, molecular biology 25g BS-6258-25G 100g BS-6258-100G www.calibrescientific.com 57

life sciences Electrophoresis Elite DNA Ladders DNA Electrophoresis Overview: • Premixed with loading dye and ready to use • Clear band separation for accurate size determination • Volumes available: 100 µl to up to 25 ml. 100 bp: • 10 bands ranging from 100 to 1,000 bp • High intensity reference band at 500 bp contains two dyes: bromophenol blue and xylene cyanole which serve as visual aids to monitor the progress of migration 1 kb: • 11 bands ranging from 500 to 10,000 bp • High intensity reference band at 1,000 bp contains two dyes: orange G loading dye and xylene cyanole which serve as visual aids to monitor the progress of migration Direct Order Link ORDERING INFORMATION Description: UOM: PN: Elite 100 bp DNA ladder 100 µl Elite 1 kb DNA Ladder 100 µl PAL-E-100BP-100 PAL-E-1KB-100 58 www.calibrescientific.com

life sciences Electrophoresis Quick Coomassie Stain Protein Electrophoresis Overview: • Highly sensitive: 5 – 25 ng bands resolved. • Rapid: Protein bands appear after minutes – Fully stained after 1 hour. • Non-hazardous: No organic solvents, no phosphoric acid, and no cloudy precipitants. • Accurate protein quantification: Low background – Linear staining range • Re-useable: Up to 3 times • Storage: Shelf Life of 18 months at 2 – 25°C • Easy to use: no fix or de-stain, just immerse the gel in stain (25 ml for standard mini gels) then store in DI water. Direct Order Link ORDERING INFORMATION Description: UOM: PN: Quick Coomassie Stain 30ml Quick Coomassie Stain PAL-GEN-QC-Stain-30ml Quick Coomassie Stain 1L PAL-GEN-QC-Stain-1L 3L PAL-GEN-QC-Stain-3L QC Stain vs Competitor. Rapid testing. Faster than the leading competitor www.calibrescientific.com 59

life sciences Electrophoresis Pre-Stained Protein Ladders Protein Electrophoresis Overview: • Volumes available: 0.5ml to 25ml. The Elite Pre-stained Protein Ladder • High-intensity, 3-colour molecular weight determination with 10 pre-stained proteins Ultra-clear and expanded molecular weight range (6.5 – 270 kDa) • Clear separation for high molecular weight proteins • Up to 100% electroblot transfer efficiency (Seema Qamar, CIMR, Cambridge University 2018) • LICOR-compatible The Eco Pre-stained Protein Ladder • High-intensity 3-colour protein standard with 9 pre-stained proteins • Covering a wide range of molecular weights from 15 – 180 kDa. • Designed for monitoring protein separation during SDS-PAGE, verifying Western transfer efficiency on membranes, and for approximating the size of proteins. Direct Order Link Elite Pre-Stained Protein Ladder ORDERING INFORMATION Description: UOM: PN: Elite Pre-stained Protein Ladder 0.5 ml Eco Pre-stained Protein Ladder 0.5 ml PAL-EPL-500 PAL-ECL-500 60 www.calibrescientific.com

life sciences Western Blot Animal Sera for Blocking Blocking Reagents Overview: • Used for blocking non-specific binding. • Goat, Donkey, Horse, Rabbit and Mouse sera available • Suitable for both Western Blot and IHC (validated for formalin-fixed paraffin embedded as well as frozen sections) • Heat treated at 56°C (for inactivation of viruses) • 2 mL and 10 mL as well as customized filling and pre-treatment available • In stock, short delivery time • Manufactured in Germany Direct Order Link 10 ml and 2 ml vials of Normal Horse serum. ORDERING INFORMATION PN: Description: UOM: LIN-ENH9010-10 HISTOPRIME Normal Horse Serum, w/Preservative (0.08% NaN3), Non Sterile 10 ml LIN-ENH9010-2 HISTOPRIME Normal Horse Serum, w/Preservative (0.08% NaN3), Non Sterile 2 ml LIN-END9010-10 HISTOPRIME Normal Donkey Serum, w/Preservative (0.08% NaN3); Non Sterile 10 ml LIN-END9010-2 HISTOPRIME Normal Donkey Serum, w/Preservative (0.08% NaN3); Non Sterile 2 ml LIN-ENG9010-10 HISTOPRIME Normal Goat Serum, w/Preservative (0.08% NaN3); Non Sterile 10 ml LIN-ENG9010-2 HISTOPRIME Normal Goat Serum, w/Preservative (0.08% NaN3); Non Sterile 2 ml LIN-ENK9010-10 HISTOPRIME Normal Rabbit Serum, w/Preservative (0.08% NaN3); Non Sterile 10 ml LIN-ENK9010-2 HISTOPRIME Normal Rabbit Serum, w/Preservative (0.08% NaN3); Non Sterile 2 ml LIN-ENM9010-10 HISTOPRIME Normal Mouse Serum, w/Preservative (0.08% NaN3), Non Sterile 10 ml LIN-ENM9010-2 HISTOPRIME Normal Mouse Serum, w/Preservative (0.08% NaN3), Non Sterile 2 ml www.calibrescientific.com 61

life sciences Western Blot Anti-His-Tag Antibodies Antibodies Overview: • Detects HIS6-tag at any position (N-/C-terminal as well as internal) • Mouse monoclonal IgG1 clone 13/45/31-2 • Great for WB, ELISA, IHC, Flow Cytometry, Electron Microscopy • High affinity with a BIACORETM value of 3x 10-10M • High sensitivity of up to 1 ng protein • More than 100 scientific references with mentions • Different formats and conjugations available Direct Order Link Anti-His Epitope Tag (rec) from Mouse (13/45/31), unconjugated, showing sensitivity of up to 1ng. ORDERING INFORMATION Description: UOM: PN: 100 µg Anti-His Epitope Tag (rec) from Mouse (13/45/31), DNA-DIA-900-100 unconjugated 200 µg DNA-DIA-900-200 DNA-DIA-910-1MG-ABF Anti-His Epitope Tag (rec) from Mouse (13/45/31) 1 mg DNA-DIA-900 FITC unconjugated 100 DNA-DIA-920 BIOT Test Anti-His Epitope Tag (rec) from Mouse (13/45/31) 100 µg unconjugated (Azide-BSA free) Anti-His Epitope Tag (rec) from Mouse (13/45/31) FITC conjugated Anti-His Epitope Tag (rec) from Mouse (13/45/31) Biotin conjugated 62 www.calibrescientific.com

life sciences PCR Purification EnzSAP™ PCR Clean-up Reagent Overview: The EnzSAP™ Reagent is comprised of two hydrolytic enzymes, recombinant Shrimp Alkaline Phosphatase (rSAP) and Exonuclease I (Exo I). The combination of these enzymes ensures complete dephosphorylation of unincorporated dNTPs and degradation of residual primers, enabling Sanger Sequencing and other downstream applications. • Simple 15-minute protocol with minimum hands-on time o Add Directly to the PCR product o Degrades excess primers and completely inactivates dNTPs • Ideal for sequential enzymatic reactions o No need to change reaction buffer • Developed for use with robotic pipettors o Automatable solution for high volume labs • 100% Sample Recovery o No loss of valuable samples Direct Order Link ORDERING INFORMATION Description: UOM: PN: 500rxns 2000rxns EDG-EnzSAP-500 EnzSAP™ Enzymatic PCR Purification (Two-Tube Format) EDG-EnzSAP-2000 EnzSAP™ Enzymatic PCR Purification (Two-Tube Format) www.calibrescientific.com 63

life sciences PCR Purification QuickStep™2 PCR Purification Kit Overview: The QuickStep2 PCR Purification Kit delivers highly purified DNA that can be immediately used for further amplification, cloning and sequencing reactions. The kit consists of two purifying reagents: • An improved patented SOPE™ (Solid phase Oligo/Protein Elimination) Resin that binds primers, ssDNA, enzymes, and other proteins. • The PERFORMA® Gel Filtration Cartridge that eliminates up to 99% of salts, buffers, dNTPs, and other small molecules. QuickStep™2 96-Well PCR Purification Kit also available. Direct Order Link ORDERING INFORMATION PN: Description: UOM: 36/pk EDG-33617 QuickStep™ 2 PCR Purification Kit 108/pk 2 plates EDG-92159 QuickStep™ 2 PCR Purification Kit 10 Plates EDG-61303 QuickStep™ 2 96 Well-PCR Purification Kit EDG-97670 QuickStep™ 2 96 Well-PCR Purification Kit 64 www.calibrescientific.com

life sciences PCR Purification Performa Spin Columns Overview: • A convenient and simple method to purify DNA/RNA samples using a fully hydrated gel matrix • Effectively remove salts, amino acids, nucleotides, traces of solvents and other low molecular weight materials from nucleic acid fragments greater than 20 bases o Broad Fragment Size: Clean up DNA fragments as large as 50 kb o Excellent Reproducible Recovery: Up to 95% recovery depending on fragment size o Quick 2-Spin Protocol: Start to finish in only 5 minutes o R eady For Immediate Use: Sample is eluted and ready for downstream applications. Applications: • Purification of Labeled DNA: Eliminate unincorporated labeled nucleotides and probes • Desalting: Removes up to 99.99% of salts and other small molecules • Alternative Method to Alcohol Precipitation: 99% removal of trace chloroform and phenol, as well as DMSO • RNA PAGE Purification: Recover 80-90% of small RNAs from denaturing PAGE gels Direct Order Link ORDERING INFORMATION Description: UOM: PN: Performa® Spin Columns 36/pk Performa® Spin Columns 108/pk EDG-73328 EDG-13266 www.calibrescientific.com 65

life sciences PCR Purification ExcelaPure™ Overview: • 96-Well ultra-filtration PCR Purification Kits • Simple, automatable, high-throughput method for purifying PCR reactions using UF o Excess primer, primer-dimers, dNTPs, and salts efficiently removed o Contaminants filtered to waste under vacuum pressure or centrifugation while purified PCR products are retained on the membrane o Purified products are recovered after a quick elution and ready for immediate use o Optimized for varying sample volumes: 50-300 µl o Process manual or automated; fits onto any standard vacuum manifold and liquid handling instrument. Direct Order Link ORDERING INFORMATION PN: Description: UOM: 1 Plate EDG-39614 ExcelaPure 96-Well UF PCR Purification Kit 10 Plates EDG-84900 ExcelaPure 96-Well UF PCR Purification Kit 66 www.calibrescientific.com

life sciences Surfectants & Detergents Anapoe-X-100 (TRITON X-100) Detergents Overview: • Purified industrial detergent. • Low oxidant (≤ 20 μM of equivalent peroxide). • Supplied in a 10% (w/v) solution under argon gas. • Great for cell lysis, membrane protein solubilization Direct Order Link ORDERING INFORMATION Description: UOM: PN: ANAPOE-X-100 5X10 ML ANAPOE-X-100 10X10 ML ANA-APX100 5X10 ML ANAPOE-X-100 50X1 ML ANA-APX100 10X10 ML ANAPOE-X-100 500 ML ANA-APX100 50X1 ML ANA-APX100 500 ML Left: scheme of liposomes with target protein VAP-A being formed by removing Anapoe X-100 from protein/lipid/detergent mixture. Right: cryo-EM images of resulting liposomes. From Mora, E. de la et al. Nat Commun 12, 3459 (2021). A CALIBRE SCIENTIFIC COMPANY www.calibrescientific.com 67

life sciences Surfectants & Detergents n-Dodecyl-β-D-Maltoside (DDM) Detergents Overview: • #1 detergent for membrane protein solubilization and purification [1]. • Low CMC. • Highest purity on the market – anagrade, ≥ 99% by HPLC analysis. Direct Order Link ORDERING INFORMATION Description: UOM: PN: DDM, anagrade 1g DDM, anagrade 5g ANA-D310 1 GM DDM, anagrade 25g ANA-D310 5 GM ANA-D310 25 GM [1] Detergents most frequently used for membrane protein solubilization; DDM is #1 accounting for ~50% of cases. Adapted from Choy, B. C., et al. Biochimica Et Biophysica Acta Bba - Biomembr 1863, 183533 (2021). A CALIBRE SCIENTIFIC COMPANY 68 www.calibrescientific.com

life sciences Surfectants & Detergents Sodium Dodecyl Sulfate Detergents Overview: • Used in important lab applications: protein SDS-PAGE gels, cell lysis • Solubilizer and emulsifier in pharma and food industries • Surfactant in cleaning and cosmetics Direct Order Link ORDERING INFORMATION Description: UOM: PN: Sodium Dodecyl Sulfate 100 g Sodium Dodecyl Sulfate 500 g ANA-S1001 100 GM Sodium Dodecyl Sulfate 1 kg ANA-S1001 500 GM ANA-S1001 1 KG A CALIBRE SCIENTIFIC COMPANY www.calibrescientific.com 69

life sciences Surfectants & Detergents Styrene Maleic Anhydride Nanodisc-forming Polymers) Detergents Overview: • Nanodiscs allow structural as well as biophysical studies of membrane proteins in their native lipid environment • Directly solubilize membrane proteins in absence of detergents • Stable in a variety of pH and at up to 200 mM Ca2+ or Mg2+ Direct Order Link ORDERING INFORMATION Description: UOM: PN: SMA-QA 250 mg SMA-QA 500 mg ANA-SMA-QA 250 MG SMA-QA ANA-SMA-QA 500 MG 1g ANA-SMA-QA 1 G From: Krishnarjuna B, et al. 2020. Chem Commun 56 6511–4 A CALIBRE SCIENTIFIC COMPANY 70 www.calibrescientific.com

life sciences Surfectants & Detergents APEEH9 (Purified from ECOSURF™ EH-9) Detergents Overview: • Biodegradable substitute for environmentally harmful Polyoxyethylene detergents (like Triton, Brij, Thesit, Lubrol) • Original formulation pre-approved by the US EPA to meet the criteria of their Safer Choice label • Purified from EcoSurf™ EH-9, Anapoe® grade (≤ 20 μM of equivalent peroxide) • Promising for structural biology, general biochemistry as well as molecular biology applications Direct Order Link ORDERING INFORMATION Description: UOM: PN: Anapoe APEEH9 10X10 ML Anapoe APEEH9 50X1 ML ANA-APEEH9 10X10 ML Anapoe APEEH9 5X10 ML ANA-APEEH9 50X1 ML ANA-APEEH9 5X10 ML Anapoe® detergents in XRD protein structures Left: UDP glucose: glycoprotein glucosyltransferase; Anapoe-C12E8 in cyan, from Zhu et al. Sci Rep 4, 7322 (2014)]. Right: P450 domain of M. capsulatus CYP51fx; Anapoe-X-114 in cyan, from Lamb et al. Mol Biol Evol 38, msaa260- (2020). A CALIBRE SCIENTIFIC COMPANY www.calibrescientific.com 71

life sciences Surfectants & Detergents Nonionic Detergents & Surfactants Membrane Protein Detergents and Surfactants Overview: • Most detergents available in Anagrade and Sol-grade. Anagrade detergents are the highest purity option and are typically >99% pure. Solgrade may have slightly higher levels of contaminants and generally have a purity level of around 95-98%. • Maltoside and glucoside detergent classes available in highly alpha and low alpha anomer compositions Direct Order Link ORDERING INFORMATION PN: Description: UOM: ANA-D322 1 GM n-Decyl-β-D-Maltopyranoside, Anagrade (Purity: ≥ 99% β+α) 1g ANA-D322 5 GM n-Decyl-β-D-Maltopyranoside, Anagrade (Purity: ≥ 99% β+α) 5g ANA-D322 25 GM n-Decyl-β-D-Maltopyranoside, Anagrade (Purity: ≥ 99% β+α) 25g 1g ANA-NG310 1 GM Lauryl Maltose Neopentyl Glycol (Purity: ≥98% all anomers) 5g 25g ANA-NG310 5 GM Lauryl Maltose Neopentyl Glycol (Purity: ≥98% all anomers) 1g 1g ANA-NG310 25 GM Lauryl Maltose Neopentyl Glycol (Purity: ≥98% all anomers) 1g ANA-O311 1 GM n-Octyl-β-D-Glucopyranoside, Anagrade (Purity: ≥ 99% β+α) 500mg 1g ANA-O311 5 GM n-Octyl-β-D-Glucopyranoside, Anagrade (Purity: ≥ 99% β+α) 5g 25g ANA-O311 25 GM n-Octyl-β-D-Glucopyranoside, Anagrade (Purity: ≥ 99% β+α) ANA-GDN101 500 MG Glyco-diosgenin; Synthetic substitute for Digitonin (Purity: ≥ 97%) ANA-GDN101 1 G Glyco-diosgenin; Synthetic substitute for Digitonin (Purity: ≥ 97%) ANA-GDN101 5 G Glyco-diosgenin; Synthetic substitute for Digitonin (Purity: ≥ 97%) ANA-GDN101 25 G Glyco-diosgenin; Synthetic substitute for Digitonin (Purity: ≥ 97%) A CALIBRE SCIENTIFIC COMPANY 72 www.calibrescientific.com

life sciences Surfectants & Detergents Nonionic Detergents & Surfactants (continued) Membrane Protein Detergents and Surfactants ORDERING INFORMATION PN: Description: UOM: 5g ANA-AZ314 5 GM Anzergent 3-14, Analytical Grade (Purity: ≥ 98%) 25g ANA-AZ314 25 GM Anzergent 3-14, Analytical Grade (Purity: ≥ 98%) 100g 1g ANA-AZ314 100 GM Anzergent 3-14, Analytical Grade (Purity: ≥ 98%) 5g 25g ANA-F308 1 GM Fos-Choline-12, Anagrade (Purity: ≥ 99%) 50mg ANA-F308 5 GM Fos-Choline-12, Anagrade (Purity: ≥ 99%) 100mg 500mg ANA-F308 25 GM Fos-Choline-12, Anagrade (Purity: ≥ 99%) 1g ANA-A835 50 MG Amphipol (maintains membrane proteins solubilized w/o detergent) 5g 25g ANA-A835 100 MG Amphipol (maintains membrane proteins solubilized w/o detergent) ANA-A835 500 MG Amphipol (maintains membrane proteins solubilized w/o detergent) ANA-CH210 1 GM Cholesteryl Hemisuccinate Tris Salt ANA-CH210 5 GM Cholesteryl Hemisuccinate Tris Salt ANA-CH210 25 GM Cholesteryl Hemisuccinate Tris Salt Other products by Anatrace: • Detergents • Maltosides, Glucosides, CYMALs, Amine Oxides, Neopentyl glycols, etc. • Lipids • Cholesterols, Fos-Choline, etc. • Amphipols • A8-35, PMAL-C8, biotinylated amphipols, etc. • Nanodiscs • SMAs, DIBMA • Anapoe • ultrapure X-100, C12E8, etc. • LCP • Kits…and more http://www.anatrace.com www.calibrescientific.com 73

life sciences Structural Biology Morpheus® Crystallization Screens X-ray Crystallography Overview: • Suite includes: Morpheus®, Morpheus® II, Morpheus® III, Morpheus® Fusion. • Derived from extensive data mining of PDB database. • Simple and effective screens covering a range of pH, PEGs and additives. • Unique low molecular weight ligands/additives promote both initial crystal formation and lattice stability. • Innovative buffer systems included. • All conditions are cryo-protected. • Ideal for both membrane proteins (contains PEGS and low salt), and soluble proteins. • Morpheus® Fusion allows 4 crystallization variables sampled simultaneously: buffer system, precipitant mix, 2 additive mixes • Available in 10 mL tube and 96 condition deep well block formats. • All conditions and/or stock reagents available separately! Direct Order Link ORDERING INFORMATION Description: UOM: PN: Morpheus Fusion 96 x 10ml Morpheus Fusion 96 x 1ml MDL-MD1-129 Morpheus Fusion 96 x 100ul MDL-MD1-130 Morpheus single reagent MDL-MD1-130-FX 100ml MDL-MDSR-46 Crystals of a human retinoblastoma-binding protein, obtained during preliminary trials of Morpheus Fusion at the LMB, and the corresponding diffraction pattern (1.6 Å) collected at Diamond Light Source. 74 www.calibrescientific.com

life sciences Structural Biology Protein Stability Screens Cryo-EM, X-Ray Crystallography Overview: • The use of protein stability screen in at the start of any structure determination project is vital to establish stabilizing conditions and eliminate undesirable specific interactions. • A more stable protein often has better protease resistance, greater solubility and improved crystal growth or resolution in Cryo-EM. • Screen is used with thermal shift assay • Provides information on the effect various factors (pH, salts, etc) have on the thermal denaturation of a protein, which is linked to protein stability. • Molecular Dimensions supply RUBIC and Durham protein stability screens. These two screens are complementary to one another and for optimum results, both should be used together. Direct Order Link Thermal shift protein stability assay ORDERING INFORMATION Description: UOM: PN: Durham pH Screen 96 x 0.5 ml Durham Salt Screen MDL-MD1-101 Durham Osmolyte Screen 96 x 0.5 ml MDL-MD1-102 RUBIC Buffer Screen MDL-MD1-120 96 x 0.5 ml MDL-MD1-96 RUBIC Additive Screen 96 x 0.5 ml MDL-MD1-97 96 x 0.25 mL 24 x 1.5 mL 5X buffers www.calibrescientific.com 75

life sciences Structural Biology Cryocrystallography Loops Cryo-EM, X-Ray Crystallography Overview: • Mounted Litholoops offer excellent reproducibility with standard distances from the base of the loop to the end of the mounting pin, making crystal centering easier. • Extended lifetime achieved by easy cleaning in an ultrasonic bath. • Etched from polyamide resin yielding practically zero background diffraction. • Fast and simple retrieval of crystals from drop, even in viscous solutions. Low hydrophobicity minimizes the volume of cryoprotectant retained. • Best of Both Worlds - very strong yet flexible in the drop, rigidity increases upon freezing giving solid mechanical stability during data collection. • Available in round, elliptical, and mesh; size range 0.02-1.0mm • Cryomount sets available (mounted in CryoCaps and Magnetic CryoVials – everything traceable) Direct Order Link ORDERING INFORMATION Description: UOM: PN: Mounted LithoLoops 0.3 mm 20 CryoMount Set (0.3mm Round) 20 MDL-MD7-138 MDL- MD7-138CM Mounted Round LithoLoop 76 www.calibrescientific.com

life sciences Structural Biology Cryoelectron Microscopy Grids Cryo-EM, X-Ray Crystallography Overview: • Gold grids: reduced beam-induced motion during imaging; biocompatible; durable • C-FLAT Carbon Grids: allow thinner and more even ice; ready to use (no cleaning needed); wide range of hole (1-4µm) and mesh (200-400) sizes; available in copper or gold; affordable. Direct Order Link C-FLAT Carbon Grid: ultra-flat carbon support ORDERING INFORMATION Description: UOM: PN: Au-Flat 0.6/1.0 Cryo-EM Grid - Gold 5 Au-Flat 0.6/1.0 Cryo-EM Grid - Gold 50 GF-0.6/1.0-3Au-45nm-5 C-Flat 1.2/1.3 Cryo-EM Grid - Gold 50 GF-0.6/1.0-3Au-45nm-50 CF-1.2/1.3-2AU-50 www.calibrescientific.com 77

life sciences Structural Biology NeXtal EasyXtal 15-Well Plates X-Ray Crystallography Overview: • Used for manual setup of hanging-drop protein crystallization trials. • Excellent optical properties and SBS footprint format makes the plates suitable for both manual and automatic visualization systems. • Available with standard, X-Seal, DropGuard, or DropGuard X-Seal Crystallization Supports. • The X-Seal, which is made from an advanced O-ring material, minimizes evaporation and prolongs the reuse of reservoir solutions. • EasyXtal DropGuard (DG) Supports enable easy setup of multiple drops per well and eliminate problems of drop spreading while using organic chemicals such as MPD or detergents. • Three cavities in each DG support can be used to accommodate 1 μl + 1 μl drops or 2 μl + 2 μl drops. The cavities isolate drops and prevent contact among them, enabling easy recovery of crystals without disrupting neighboring drops. Direct Order Link ORDERING INFORMATION Description: UOM: PN: EasyXtal 15-well Tool (20) 20 units EasyXtal 15-well Tool (100) 100 units NXT-132006 EasyXtal 15-Well Tool X-Seal (20) 20 units NXT-132007 EasyXtal 15-Well Tool X-Seal (100) 100 units NXT-132008 EasyXtal 15-well DG-Tool (20) 20 units NXT-132009 EasyXtal 15-well DG-Tool (100) 100 units NXT-132106 EasyXtal 15-Well DG-Tool X-Seal (20) 20 units NXT-132107 EasyXtal 15-Well DG-Tool X-Seal (100) 100 units NXT-132108 NXT-132109 78 www.calibrescientific.com

life sciences HPLC Chromatography RNA Separation Columns HPLC columns Overview: • High resolution enables precise separation and quantification of RNA fragments and contaminants • Widest target RNA size range: same column works for range of 15 – 8,000 bases • No carryover due to non-porous resin • Supports HPLC, UHPLC, Flash modes • Possible to couple with MS Direct Order Link ORDERING INFORMATION Description: UOM: PN: 1 column 1 column RPC-99-3550 RiboSep™ RNA Column, 4.6 x 50 mm, analytical 1 column 1 column RPC-99-3810 RiboSep™ RNA Column, 7.8 x 50 mm, analytical 1 column RPC-99-2110 RiboSep™ RNA Semi Prep, 21.2 x 100 mm, semi-prep RPC-99-3010 RiboSep™ RNA Semi Prep, 30 x 100 mm, semi-prep RPC-99-3015 RiboSep™ RNA Semi Prep, 30 x 150 mm, semi-prep RNA chromatograms: siRNA range (left) and high-range RNA separation. www.calibrescientific.com 79

life sciences HPLC Chromatography Ligand Exchange Columns HPLC columns Overview: • Best columns for precise separation of carbohydrates and organic acids • Wide application range: plasma/plant extracts, biofuels (801FA), food (syrup, cookie, wine, etc.) • Specialized columns for “quick and dirty” (CHO 87C) as well as high-resolution precision (CHO 682) runs • Multiple metal ligands offered to efficiently separate complex sugar matrices Direct Order Link ORDERING INFORMATION Description: UOM: PN: 1 column 1 column CHO-99-9854 CarboSep CHO 682, Lead Form, 7.8 x 300 mm CHO-99-9860 CarboSep CHO 87C, Calcium Form, 7.8 x 300 mm CHO 682 (left) and CHO 87C (right) separations. 80 www.calibrescientific.com

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life sciences HPLC Chromatography Smart Healthy Caps Chromatography Supplies Overview: • One-way air check valve • Reduce evaporation, maintain accuracy of HPLC solutions • Protect people in the lab from potentially toxic fumes • Made of inert materials, resistant to chemicals • Available in a variety of thread sizes and configurations Air In Solvent Out Direct Order Link ORDERING INFORMATION Description: UOM: PN: 1 cap 1 cap AIT-SHC-2 SmartHealthyCaps GL45 of 2 outlets (1/8\")+ 1 air valve AIT-SHC-6 SmartHealthyCaps GL45 of 6 outlets(1/8\")+ 1 air valve 82 www.calibrescientific.com

life sciences HPLC Chromatography Smart Waste Caps Chromatography Supplies Overview: • Airtight system to safely contain all waste chemicals • Protecting the laboratory staff • Activated charcoal filter allows for clean ventilation (cartridge sold separately) • Filters out 98% of the solvent vapors released by the waste • Available in various thread sizes, tube diameters • Compatible with all major HPLC instruments. Clean, Safe Air Out Waste Solvent In Direct Order Link ORDERING INFORMATION PN: Description: UOM: AIT-SW60-1-C-2L SmartWasteCap S60 with 1 charcoal cartridge filter replacement 1 + 2 Barbed Fitting (6-9 mm)+ 1 entry (1/8\" or 1/16\") cap AIT-FC-25 Charcoal cartridge filter (exhaust filter) 25 gr 1 (service life: 3 months) cartridge www.calibrescientific.com 83

life sciences HPLC Chromatography Autosampler Vials & Caps Chromatography Supplies Overview: • Overview text to go here • Overview text to go here • Overview text to go here • Overview text to go here 6mm Inserts Screw, Crimp, Snap IO25BS-629 IO25-630 Screw Caps C395-09B ND9 Screw Vials Crimp Caps Amber or Clear C141-11B VT009M-1232A VT009M-1232 Snap Caps C270CS-11 84 www.calibrescientific.com

life sciences HPLC Chromatography Autosampler Vials & Caps Chromatography Supplies ORDERING INFORMATION Descripion Colour UOM Insert Size SKU ND9 Screw Cap Vials & Caps VT009M-1232 2mL, 12x32mm 9mm Screw Cap, w/ Marking Spot Clear 100 6mm VT009M-1232A 2mL, 12x32mm 9mm Screw Cap, w/ Marking Spot Amber 100 6mm C395-09B Blue* 100 N/A C397-09B Blue ND9 Ribbed Screw Cap, Press-Fit Blue* 100 N/A Red PTFE/Silicone Septa Blue ND9 Ribbed Screw Cap, Press-Fit Red PTFE/Silicone Septa w/ Slit *Also available in Red, Green and Black ND11 Crimp Cap Vials & Caps VT011LM-1232 2mL, 12x32mm 11mm Crimp Cap, w/ Marking Spot Clear 100 6mm VT011M-1232A 2mL, 12x32mm 11mm Crimp Cap, w/ Marking Spot Amber 100 6mm C141-11B Blue* 100 N/A C150-11 Blue ND11 Crimp Cap, PTFE/Red Silver 100 N/A Rubber Silicone Septa Silver ND11 Crimp Cap, PTFE/Silicone Septa *Also available in Red, Green and Gold ND11 Snap Cap Vials & Caps VT011SM-1232 2mL, 12x32mm 11mm Snap Cap, w/ Marking Spot Clear 100 6mm VT11SM-1232A 2mL, 12x32mm 11mm Snap Cap, w/ Marking Spot Amber 100 6mm C250-11 Clear ND11 Snap Cap, Red/PTFE Silicone Septa Clear 100 N/A C270CS-11 Clear ND11 Snap Cap, Red/PTFE Silicone Septa w/ Slit Clear 100 N/A 6mm Inserts I025BS-629 250µL Glass Conical Insert Bottom Spring, 6x29mm Clear 100 250µl I025-630 350µL Glass Flat Bottom Insert, 6x30mm Clear 100 350µl www.calibrescientific.com 85

life sciences Headspace Screw Caps CHR-1414 HPLC Chromatography Headspce Crimp Caps Headspace Vials & Caps C141-11B Chromatography Supplies Round or Flat Bottom Overview: • Overview text to go here • Overview text to go here • Overview text to go here • Overview text to go here Volume (20mL & 10mL) VXX020A-2375X VX018-2346CTC Amber or Clear VXX020A-2375X VX0020-2375X 86 www.calibrescientific.com

life sciences HPLC Chromatography Headspace Vials & Caps Chromatography Supplies ORDERING INFORMATION Descripion Colour UOM Insert Size SKU ND18 Screw Cap Headspace Vials VXX018-2375S 20ml, 23x75mm 18mm Screw Cap, Headspace Vial Clear 100 20mL VXX018A-2375S 20ml, 23x75mm 18mm Screw Cap, Headspace Vial Amber 100 20mL VXX018-2375CTC 20mL, 23x75mm 18mm Screw Cap, Clear 100 20mL Round Bottom Headspace vial VXX018-2346S 10ml, 23x75mm 18mm Screw Cap, Headspace Vial Clear 100 10mL VXX018A-2346S 10ml, 23 x 75mm 18mm Screw Cap, Headspace Vial Amber 100 10mL VX0018-2346CTC 10mL, 23x46mm 18mm Screw Cap, Clear 100 10mL Round Bottom Headspace Vial CHR-1414 ND18 Screw Cap, Blue PTFE / Silicone Septa Silver 100 N/A CLS-1412 ND18 Screw Cap, Red PTFE / Silicone Septa Silver 100 N/A CHR-1416 ND18 Screw Cap, Grey PTFE / Red Butyl Septa Silver 100 N/A ND20 Crimp Cap Headspace Vials VXX020-2375X 20ml, 23x75mm 20mm Crimp Cap, Headspace Vial Clear 100 20mL VXX020A-2375X 20ml, 23x75mm 20mm Crimp Cap, Headspace Vial Amber 100 20mL VXX020-2375CTC 20mL, 23x75mm 20mm Crimp Cap, Round Bottom Clear 100 20mL Headspace Vial VX0020-2346X 10ml, 23x46mm 20mm Crimp Cap, Headspace Vial Clear 100 10mL VX0020AB-2346X 10ml, 23x46mm 20mm Crimp Cap, Round Bottom Amber 100 10mL VX0020-2346CTC Headspace Vial Clear 100 10mL 10ml, 23x46mm 20mm Crimp Cap, Round Bottom Headspace Vial CHR-0030 ND20 Crimp Cap, PTFE/Butyl Pharma-Fix Septa Silver 100 N/A CLS-9981 ND20 Crimp Cap, PTFE/Silicone Septa Silver 100 N/A CLS-1535B ND20 Magnetic Crimp Cap, Tan PTFE/Silicone Septa Blue* 100 N/A *Also available in Red, Green, Gold and Silver www.calibrescientific.com 87

life sciences Plant Science Murashige and Skoog Basal Salt Mixture (MS) Plant Growth Media Overview: • Most widely used plant tissue culture growth medium with best pricing. • Contains the base mix established by Murashige & Skoog. • Does not contain other nutrients, gelling agents, carbohydrates, etc. • Powder is simply dissolved in the appropriate volume of water and autoclaved. For semi-solid culture systems agar is added before autoclaving. • It can be easily combined with vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture. • Used to support the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants; for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises. • Available options: Murashige & Skoog Basal Medium w/ Vitamins – Same as M524 but with vitamins; Murashige & Skoog Basal Medium w/ Gamborg Vitamins. • Available volumes: 1L to 500L. Direct Order Link ORDERING INFORMATION Description: UOM: PN: Murashige & Skoog Basal Salt Mixture, Solid 1L PTL-M524-1L Effect of GST inhibitor, NBD-Cl, and S-metolachlor on seedling growth of Amaranthus palmeri. Plants were grown on PhytoTech Labs M&S supplemented with agar. From Rangani, G., et al. Front Plant Sci 12, 652581 (2021). 88 www.calibrescientific.com

life sciences Plant Science Media Optimization Kit (DKW) Plant Growth Media Overview: • DKW-based medium for walnut (Driver & Kuniyuki 1984) is presented in a Design of Experiments (DOE) format (Niedz & Evens 2007). • Kit allows to vary NH4+, K+, NO3-, Ca2+, SO42- ions in search of best growth conditions. • Kit comes with detailed instructions for setup of 5 series of media optimization experiments as well as a spreadsheet for values plug-in. • For use plant tissue culture medium for various crops such as fruit, nut, and ornamental trees, as well as cannabis. Direct Order Link ORDERING INFORMATION Description: UOM: PN: Media Optimization Kit (DKW) 1 kit D2400 www.calibrescientific.com 89

life sciences Plant Science PhytoAx™ Plant Tissue Culture Components Overview: • A patent-pending solution for tissue culture that is used in plant media to promote axillary shoots in cannabis or other species. • With PhytoAx™, you get high fidelity tissue replication, reproducible multiplication rates, and enhanced longevity in culture. • Used in conjunction with DKW medium for optimal plant multiplication. • Working concentration is 0.5 – 1.0 mL/L. • Recommended Products: DKW Medium w/ Vitamins; Agar; D-Sucrose Direct Order Link ORDERING INFORMATION Description: UOM: PN: PhytoAx™ 10 mL PhytoAx™ 25 mL P4001 P4001 Initiate into DKW + DKW Medium PhytoAxTM 90 www.calibrescientific.com

life sciences Plant Science Agar, Micropropagation Grade Plant Tissue Culture Components Overview: • A high gel strength agar produced from the seaweed genus, Gracilaria. • One of the most commonly used gelling agents for plant tissue culture production and research. • Working concentration is 6 – 10 g/L with a common usage range of 6 – 8 g/L. • Recommended Products: MS Medium; DKW Medium; Lloyd & McCown Medium; D-Sucrose • Available package sizes: 100g to 25 kg Direct Order Link ORDERING INFORMATION Description: UOM: PN: Agar, Micropropagation Grade 100g A111 www.calibrescientific.com 91

diagnostics Calibre Scientific is a proud global provider and distributor of a range of diagnostic products including TB diagnostic kits and reagents, blood transfusion reagents together with the consumables and ancillary devices required to meet the growing needs of researchers and health care professionals. Our diagnostic portfolio spans key health and therapeutic areas, including Haematology, Microbiology and Sanger Sequencing in hospitals, reference labs, molecular labs, blood banks, physician offices and clinics. Our commitment has been to revolutionize how laboratories and clinicians do their work, by providing cutting edge diagnostic products to meet your diagnostic and laboratory product needs at every step of the way. Visit our website https://calibrescientific.com/en for more information. 92 www.calibrescientific.com

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diagnostics Haematology Blood Grouping Reagents ABO Blood Grouping Reagents • Anti-A, Anti-B and, Anti-A, B Overview: Blood grouping reagents are solutions that are used to determine human blood group antigens (such as ABO, Rhesus, Kell, and MNS blood group antigens). Our quality blood grouping reagents are manufactured in full compliance with all UK and European regulatory requirements and is supplied at optimal dilution for use with slide, tube, gel card & microplate blood grouping techniques Key Features • High-quality blood grouping reagents that are accurate and easy to use. • Show batch to batch consistency, high potency, high specificity and titres. • Products are CE marked in full compliance with the latest EC directives and British standards and BCSH/BBTS UK guidelines. Direct Order Link ORDERING INFORMATION Product Code Size Shelf Life Product Name 600010 10ml 36 mths 600000 1000ml 36 mths Anti-A Monoclonal 610010 10ml 36 mths 610000 1000ml Anti-B Monoclonal 620010 10ml 620000 1000ml Anti-A, B Monoclonal 94 www.calibrescientific.com

diagnostics Haematology Blood Grouping Reagents Rhesus Reagents (Anti-D) • Anti-D Clone 1 and Clone 2 Monoclonal • Anti-D Duoclone Monoclonal Overview: Blood grouping reagents are solutions that are used to determine human blood group antigens (such as ABO, Rhesus, Kell, and MNS blood group antigens). Our quality blood grouping reagents are manufactured in full compliance with all UK and European regulatory requirements and is supplied at optimal dilution for use with slide, tube, gel card & microplate blood grouping techniques. Direct Order Link ORDERING INFORMATION Product Code Size Shelf Life Product Name 730010 10ml 30 mths 730000 1000ml 30 mths Anti-D Clone 1 Monoclonal 710010 10ml 30 mths 710000 1000ml Anti-D Clone 2 Monoclonal 740010 10ml 740000 1000ml Anti-D Duoclone Monoclonal www.calibrescientific.com 95

diagnostics Haematology Blood Grouping Reagents ABO Blood Grouping Reagents • Anti-human Globulin reagent A.H.G Elite (Green) Overview: Polyspecific Anti-Human Globulin Elite Green reagent contains anti-human IgG derived from rabbits with non-specific activity removed by absorption. When used by recommended techniques, the reagent will cause agglutination (clumping) of red cells, carrying IgG, in the antiglobulin phase of testing. The antibodies are diluted in a buffered solution containing bovine albumin. Each reagent is supplied at optimal dilution for use with spin tube techniques. Direct Order Link ORDERING INFORMATION Product Code Size Shelf Life Product Name 435010 10ml 24 mths A.H.G Elite (Green) 96 www.calibrescientific.com

diagnostics Haematology CRP Latex Kit Overview: The CRP-latex is a rapid latex agglutination test on a slide for the detection of C-reactive protein (CRP). The reagent contains polystyrene latex particles coated with goat antiserum anti-human CRP in buffer solution. The CRP present in the patient sera reacts with the coated latex particles causing a macroscopic agglutination that can be seen visually. Kit Components • CRP - Latex (Latex particles coated with goat IgG anti-human CRP, in a buffer solution) • CRP Positive Control • CRP Negative Control Key Features • Kits are CE marked and sized to suit the frequency and nature of each test. • Available in quantities of either 50 or 100 tests • Additional bulk reagents are available in 1 litre quantities Direct Order Link ORDERING INFORMATION Product Code Size Shelf Life Product Name 850100A 100 Tests 30 Months CRP Latex Kit www.calibrescientific.com 97

diagnostics Covid-19 SARS-CoV-2 Antigen Variants • Omicron Variant (B.1.1.529) • Delta Variant (B.1.617.2) • Wild Type Overview: SARS-CoV-2 has experienced a variety of mutations in the RBD domain of the spike protein which has led to the development of numerous variants in recent months. We offer a range of antigens including the full-length SARS-CoV-2 Spike Protein and the mutant strains B.1.1.529 (Omicron Variant) and B.1.617.2(Delta Variant). The proteins are manufactured in HEK293 mammalian cells, ensuring the most authentic post- translational modifications. Key Features • All variants are full-length antigens, which are active in their native trimeric • form and stabilized in LMNG detergent. • We are the only supplier, to scale, of the native, wild-type Trimeric Spike Antigen, which has been independently validated with an accuracy of >99% in the large-scale manufacturing of COVID-19 lateral flow antigen tests in the EU. A Direct Order Link ORDERING INFORMATION Product Code Pack Size Product Name BSV-COV-PR-33 100 μg BSV-COV-PR-34 1 mg Trimeric SARS-CoV-2 BSV-COV-PR-35 10 mg Spike Protein, BSV-COV-OM-0.1 100 µg Full-length BSV-COV-OM-1 1 mg BSV-COV-OM-10 10 mg SARS-CoV-2 full-length Trimeric BSV-COV-PR-97 100 μg Spike Recombinant Antigen BSV-COV-PR-98 1 mg Omicron Variant BSV-COV-PR-99 10 mg SARS-CoV-2 full-length Trimeric Spike Recombinant Antigen Delta Variant 98 www.calibrescientific.com

diagnostics Covid-19 Elite Anti-SARS-CoV-2 Antibodies Overview: These highly-specific antibodies were raised to the four main SARS-CoV-2 targets (S1, S1-RBD, S2 and N proteins). During development, all candidate antibodies were screened against SARS and MERS to ensure each epitope was unique to SARS-CoV-2, and no cross-reactivity with pre-COVID coronaviruses was observed. Antibody pairs were also independently validated using Sandwich ELISA assays. Key Features Our antibodies are ideal for use in the development of lateral flow antigen detection tests, a staple of COVID-19 testing today! Direct Order Link ORDERING INFORMATION Product Code Concentration BSV-S1-01 1mg/ml Product Name BSV-RBD-01 1mg/ml BSV-S2-01 1mg/ml Elite Anti-SARS-CoV-2 Spike BSV-N-01 1mg/ml (S1) Monoclonal Antibody, Clone COS1α Elite Anti-SARS-CoV-2 Spike (S1-RBD) Monoclonal Antibody, Clone CORBDα Elite Anti-SARS-CoV-2 Spike (S2) Monoclonal Antibody, Clone COS2α-1 Elite Anti-SARS-CoV-2 Nucleocapsid Protein Monoclonal Antibody, Clone CONα-1 www.calibrescientific.com 99

diagnostics Microbiology QC1™ Quality Control Slides QC1 AFB Slides Overview: QC1 Slides are the easy and reliable way to validate stain quality and procedures – just open and stain. Our slides are available for multiple diagnostic methodologies including AFB, Gram, Nocardia, Cryptosporidium, Parasitology (Giardia), Mycology, Trichomonas, and our newest slide, Malaria. Direct Order Link ORDERING INFORMATION Product Name Product Code Product Description Quality 10 slides/pk ATS-0003243 High organism count 40 slides/pk per high-powered field QC1™ AFB Slides ATS-0003240 w/ simulated sputum background 40 slides/pk ATS-0003245 ATS- 0003247 Moderate organism count per high-powered field Low organism count per high-powered field 100 www.calibrescientific.com