Western Blot

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Western Blot

After the blotting process, which can take about an hour (semidry blotting) to overnight (tank blotting), the proteins are exposed on a thin surface layer for antibody detection. Besides, the free binding sites of the membrane are blocked with a protein mixture, which will not interfere with the subsequent probing with an antibody. The proteins are first detected by the primary antibody, and then detected with a secondary antibody, which recognizes this particular primary antibody. The secondary antibody is conjugated with a set of specific molecules, which can be easily detected with a subsequent development procedure with high sensitivity.

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Western Blot