Peptide Mass Fingerprinting analysis

Peptide Mass Fingerprinting analysis

• Protein• Information about Peptide Mass Fingerprinting (PMF)• MS/MS analysis vs Peptide Mass Fingerprinting (PMF)• Customer service

Protein• A pure protein is digested using a proteolytic enzyme (often trypsin) to cleave the protein (a single protein or a complex mixture of different proteins) into constituent peptides. The peptide masses are then measured very accurately by mass spectrometry and searched against a theoretical digest of all protein maintained in a database.

Peptide Mass Fingerprinting (PMF)• Peptide Mass Fingerprinting (PMF) grew from a need for a faster, more efficient method to identify proteins.• PME is employed by majority of proteomic studies.• Peptide mass fingerprinting was a rapid method that was useful in identifying known proteins as there is no requirement for a free amino terminus.

MS/MS analysis vs Peptide Mass Fingerprinting(PMF)• Although sequence information from MS/MS analysis can be used to validate PMF-based protein identification, it may not be practical when analyzing a large amount protein and when high- throughput MS/MS instrumentation is not readily available. Besides, in comparison to MS/MS analysis on a single peptide, identification of proteins using PMF has two significant advantages that are often ignored.

Customer service• For more information, please visit https://www.creative- proteomics.com/services/peptide-mass-fingerprinting-pmf.htm• Email: [email protected]• Phone: 1-631-619-7922