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Home Explore Peptide Mass Fingerprinting analysis

Peptide Mass Fingerprinting analysis

Published by Sherry Cryer, 2018-09-05 02:38:44

Description: Researchers are still working on providing a comprehensive protein identification by applying Peptide mass fingerprinting in which help shorten the time of protein identification and enhance the accuracy of protein sequencing. Creative proteomics provides you ideal results to speed up your research.

Keywords: Peptide Mass Fingerprinting analysis


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Peptide Mass Fingerprinting analysis

• Protein• Information about Peptide Mass Fingerprinting (PMF)• MS/MS analysis vs Peptide Mass Fingerprinting (PMF)• Customer service

Protein• A pure protein is digested using a proteolytic enzyme (often trypsin) to cleave the protein (a single protein or a complex mixture of different proteins) into constituent peptides. The peptide masses are then measured very accurately by mass spectrometry and searched against a theoretical digest of all protein maintained in a database.

Peptide Mass Fingerprinting (PMF)• Peptide Mass Fingerprinting (PMF) grew from a need for a faster, more efficient method to identify proteins.• PME is employed by majority of proteomic studies.• Peptide mass fingerprinting was a rapid method that was useful in identifying known proteins as there is no requirement for a free amino terminus.

MS/MS analysis vs Peptide Mass Fingerprinting(PMF)• Although sequence information from MS/MS analysis can be used to validate PMF-based protein identification, it may not be practical when analyzing a large amount protein and when high- throughput MS/MS instrumentation is not readily available. Besides, in comparison to MS/MS analysis on a single peptide, identification of proteins using PMF has two significant advantages that are often ignored.

Customer service• For more information, please visit https://www.creative-• Email: [email protected]• Phone: 1-631-619-7922

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