JC STEM Summer Lessons & Resources K-4

TEACHER MODEL AFTER SHOWING THE FIRST 58 SECO DEMONSTRATE HOW TO EXTRACT ST

ONDS OF THIS VIDEO, YOU WILL TRAWBERRY DNA.

List of Materials, 1 Material Bottle of dish soap Container of salt 15 mL Conical Tube 5ml disposable pipette* Isopropol alcohol (500 mL) Sharpie Clear Plastic Cups 44 Proprietary & Confidential

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List of Materials, 2 Material Basket of Strawberries Coffee filters* Roll of paper towels Small cups (Dixie cups)* scissors Ziplock bags* Water (500 mL) 45 Proprietary & Confidential

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Table Set Up Volunteers sta SOAP SALT Students stand 46 Proprietary & Confidential

and on this side IPA water SOAP SALT d on this side

Preparation Prior to Activity With Dispense 10 mL of water into one of the 15ml conicals. Volume ac 1 not too important. Dispense 5 mL of alcohol into one of the 15ml conicals. Volume ac not too important. Quarter the strawberries using the scissors (4 pieces) and place on 2 quarter of strawberry into separate ziplock bags. Remove stem fro strawberry. Prepare kits for Students • Ziplock bag containing strawberry piece • Conical containing 10 mls of water • Empty 15 mL conical 3 • 5 ml Disposable pipette bulb • Small cup • Coffee filter • Student Instructions • Paper towel 47 Proprietary & Confidential

h Students 10 mL 10 mL ccuracy is ccuracy is ne om

Student Activity Instructions Hand out one kit to each group Prepare Cell Lysis buffer • Add a generous pinch of salt to 15 mL conical containing water • Dissolve salt by inverting conical 10-15x • Add 10 drops of soap using 5 mL 1 disposable pipette • Carefully and slowly invert conical to dissolve thoroughly, be careful not to create bubbles Question 1: Why is detergent good at dissolving grease? What might the detergent be doing to the cells? 48 Proprietary & Confidential

Avoid Bubbles Slowly

Student Activity Instructions Prepare strawberries for DNA extraction • Mash the strawberry inside of the ziplock bag until it is a paste, use your 2 fists. • Pour the cell lysis buffer into the ziplock bag, pair with partner to do this • Gently mix the strawberry and lysis buffer together for 1-2 minutes. Gent 49 Proprietary & Confidential

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Student Activity Instructions Separate DNA from Proteins • Place coffee filter on top of Dixie cup • Slowly pour the mixture into the funnel shaped filter and allow the solution to drip through into the cup. Do not squeeze the filter as it may allow 3 proteins to also drip through • Once the solution has stopped dripping, remove the filter and place on paper towel. Discard into trash. • Add the solution in the Dixie cup to the 15 mL conical. Pinch both sides of cup to facilitate pouring. 50 Proprietary & Confidential

Reuse conical with lysis buffer

Student Activity Instructions ?m Precipitate DNA in Alcohol solution • Determine the total recovered volume from strawberry mixture in 15 mL conical by visual inspection 4 • Add an equal volume of Isopropyl alcohol slowly along the side of the 15 ml conical so that it layers on top of the strawberry mixture • Do not mix or invert the conical after adding alcohol Do not mix 51 Proprietary & Confidential

mL x

Student Activity Instructions Watch as the DNA comes out of solution! • The DNA will start to form on the interface between the strawberry 4 mixture and the alcohol. It will look like little bubbles on fine white stringy snot. Question 2: Why are we able to see the DNA after we add alcohol? 52 Proprietary & Confidential

DNA!!

How does this work!? • What does DNA stand for? • DNA stands for deoxyribonucleic acid. DNA two spirals twisting around each other. • What does DNA do? • DNA is the genetic instruction manual for ma are controlled by DNA. • Why do we use Strawberries? • Ripe strawberries are an excellent source fo and most important, strawberries have eight are octoploid), so there is a lot of DNA to iso • Why do we use the dishwashing liquid • The dishwashing liquid bursts open the cells • What does the salt do? • Helps to remove proteins that are bound to t dissolved in the aqueous layer so they don’t • Why does alcohol clump the DNA? • When molecules are insoluble (unable to be visible. DNA is not soluble in alcohol; therefo and become visible to the naked eye. 53 Proprietary & Confidential

is a molecule in the form of a double helix - aking an organism. All processes in the body or extracting DNA because they are squishy t times the amount of DNA as other fruits (they olate. d? s of the strawberries, releasing the DNA. the DNA. It also helps to keep the proteins t precipitate in the alcohol along with the DNA. e dissolved), they clump together and become ore, it makes the DNA strands clump together





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EVEN MORE FUN PLAY W https://www.kixcereal.com/kix-ceral-play-w

WITH YOUR FOOD with-your-food-berry-paintings/

ICE CREAM Strawberry Ice Cream in a Bag Homemade ice cream is more than enough this recipe takes things to a whole new level bag! It all starts with 6 simple ingredients t We opted for a fresh strawberry flavor, but chocolate or vanilla. If you’re going for van the vanilla to 2 teaspoons and the sugar to 3 get the kids involved. Once the ingredients shake vigorously until the mixture is thick a come in. Give this fun and interactive recip

to make any Southerner’s heart soar, but l. This velvety treat is made in a Ziplock that are probably in your kitchen already. t you can transform the ice cream to nilla, omit the strawberries and increase 3 tablespoons. This is an excellent recipe to are combined in the bag, you have to and chilled – and that’s where the kids pe a try and let us know what you think.

INSTRU Serves: 2 (serving size: about 1 cup) Strawberry Ice Cream in a Bag Ingredients Ingredient Checklist 4 cups ice 1/4 cup rock salt or kosher salt 1 cup half and half 2 tablespoons granulated sugar 1 teaspoon vanilla extract 1/2 cup chopped strawberries Directions Instructions Checklist Step 1 Combine ice and salt in a gallon Ziplock bag; set asi strawberries in a quart Ziploc bag; seal tightly. Plac bag in a kitchen towel. Shake vigorously until half a minutes. Remove from bag to serve. © Copyright 2021 southernliving.com. All rights reserved. Printed from https://www.southernliving.com 06/30/2021

UCTION ide. Combine half and half, sugar, vanilla, and ce quart bag inside gallon bag; seal. Cover gallon and half mixture is thick and chilled, about 7-10

Cita Beatty, A. (n.d.). The Questionnaire. For Educators – The Ques Beaty, A. (2020, February 18). Ada Twist Scientist STFS 7 01 1 Garrett, G. (2020, June 3). Scientists Ask Questions. YouTube. Harrison, V. (2020, July 14). Cece Loves Science--Read Aloud How to Make Strawberry Ice Cream in a Bag. Southern Living. https://www.southernliving.com/recipes/strawberry-ice-cream-in Inc., E. C. (2017). From Seed to Strawberry. Read From Seed https://www.getepic.com/book/40753704/from-seed-to-strawbe t&share=27425597168. Living and Nonliving Things - BrainPOP Jr. (n.d.). https://jr.brain Living and Nonliving Things - BrainPOP Jr. (n.d.). https://jr.brain Pippett kids. (n.d.). DNA coloring page. Teachers Pay Teachers https://www.teacherspayteachers.com/FreeDownload/Color-the 9. Ramsey, K. (2015, February 2). Using Science Tools. BetterLes https://betterlesson.com/lesson/626676/using-science-tools. Science Mom Extracts DNA from a Strawberry. YouTube. (2018 Science Skills - BrainPOP Jr. (n.d.). https://jr.brainpop.com/scie StGermainFamily. (2018, February 4). The Little Mouse, The R Don Wood. YouTube. https://www.youtube.com/watch?v=WO2

ations stionnaires. https://questioneers.com/for-educators/. 19. YouTube. https://youtu.be/Q7TLqqct42M. . https://www.youtube.com/watch?v=jxYJHu7x2kk. d! YouTube. https://youtu.be/IGd7pxA1WNo. (n.d.). n-bag?printview. to Strawberry on Epic. erry?utm_source=t2t&utm_medium=link&utm_campaign=conten npop.com/science/plants/livingandnonlivingthings/. npop.com/science/plants/livingandnonlivingthings/. s. e-DNA-Worksheet-Pipette-Kids-Science-Coloring-Book-534068 sson. 8, July 6). https://youtu.be/d2IBcOI22uI. ence/beascientist/scienceskills/. Red Ripe Strawberry, and the Big Hungry Bear by Audrey and 2vZi32d6U.















Strawberry DNA E Step by Step Guid Corporate Social Responsibility Proprietary & Confidential

Extraction Activity de The world leader in serving science

Lab Safety Guidelines 1. Remember to conduct yourself in a responsible manner at all times 2. Review all safety information and procedures before beginning any activity or investigation. 3. Never eat, drink, chew gum or touch your eyes while working in the lab. 4. Keep your work area clean and neat. 5. Always make sure your instructor is present when you are working in the lab. 6. Never remove chemicals or equipment from the lab area. 7. Always wear ANSI Z87.1 approved chemical splash goggles when you are conducting experiments. 8. Be aware of the location of all safety equipment, including eye-wash stations, deluge showers, fire extinguishers and safety blankets. 9. When laboratory aprons are required, be sure to keep your apron tied throughout the entire activity. 2 Proprietary & Confidential



List of Materials, 2 Material Basket of Strawberries Coffee filters* Roll of paper towels Small cups (Dixie cups)* scissors Ziplock bags* Water (500 mL) 4 Proprietary & Confidential

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