JC STEM Summer Lessons & Resources K-4

List of Materials, 1 Material Bottle of dish soap Container of salt 15 mL Conical Tube 5ml disposable pipette* Isopropol alcohol (500 mL) Sharpie Clear Plastic Cups 3 Proprietary & Confidential

Qty 1 1 2 1 1 1 2

Table Set Up Volunteers sta SOAP SALT Students stan 5 Proprietary & Confidential

and on this side IPA water SOAP SALT nd on this side

Preparation Prior to Activity With Dispense 10 mL of water into one of the 15ml conicals. Volume ac 1 not too important. Dispense 5 mL of alcohol into one of the 15ml conicals. Volume ac not too important. Quarter the strawberries using the scissors (4 pieces) and place on 2 quarter of strawberry into separate ziplock bags. Remove stem fro strawberry. Prepare kits for Students • Ziplock bag containing strawberry piece • Conical containing 10 mls of water • Empty 15 mL conical 3 • 5 ml Disposable pipette bulb • Small cup • Coffee filter • Student Instructions • Paper towel 6 Proprietary & Confidential

h Students 10 mL 10 mL ccuracy is ccuracy is ne om

Student Activity Instructions Hand out one kit to each group Prepare Cell Lysis buffer • Add a generous pinch of salt to 15 mL conical containing water • Dissolve salt by inverting conical 10-15x • Add 10 drops of soap using 5 mL 1 disposable pipette • Carefully and slowly invert conical to dissolve thoroughly, be careful not to create bubbles Question 1: Why is detergent good at dissolving grease? What might the detergent be doing to the cells? 7 Proprietary & Confidential

Avoid Bubbles Slowly

Student Activity Instructions Prepare strawberries for DNA extraction • Mash the strawberry inside of the ziplock bag until it is a paste, use your 2 fists. • Pour the cell lysis buffer into the ziplock bag, pair with partner to do this • Gently mix the strawberry and lysis buffer together for 1-2 minutes. Gent 8 Proprietary & Confidential

tly

Student Activity Instructions Separate DNA from Proteins • Place coffee filter on top of Dixie cup • Slowly pour the mixture into the funnel shaped filter and allow the solution to drip through into the cup. Do not squeeze the filter as it may allow 3 proteins to also drip through • Once the solution has stopped dripping, remove the filter and place on paper towel. Discard into trash. • Add the solution in the Dixie cup to the 15 mL conical. Pinch both sides of cup to facilitate pouring. 9 Proprietary & Confidential

Reuse conical with lysis buffer

Student Activity Instructions ?m Precipitate DNA in Alcohol solution • Determine the total recovered volume from strawberry mixture in 15 mL conical by visual inspection 4 • Add an equal volume of Isopropyl alcohol slowly along the side of the 15 ml conical so that it layers on top of the strawberry mixture • Do not mix or invert the conical after adding alcohol Do not mix 10 Proprietary & Confidential

mL x

Student Activity Instructions Watch as the DNA comes out of solution! • The DNA will start to form on the interface between the strawberry 4 mixture and the alcohol. It will look like little bubbles on fine white stringy snot. Question 2: Why are we able to see the DNA after we add alcohol? 11 Proprietary & Confidential



How does this work!? • What does DNA stand for? • DNA stands for deoxyribonucleic acid. DNA two spirals twisting around each other. • What does DNA do? • DNA is the genetic instruction manual for ma are controlled by DNA. • Why do we use Strawberries? • Ripe strawberries are an excellent source fo and most important, strawberries have eight are octoploid), so there is a lot of DNA to iso • Why do we use the dishwashing liquid • The dishwashing liquid bursts open the cells • What does the salt do? • Helps to remove proteins that are bound to dissolved in the aqueous layer so they don’t • Why does alcohol clump the DNA? • When molecules are insoluble (unable to be visible. DNA is not soluble in alcohol; therefo and become visible to the naked eye. 12 Proprietary & Confidential

A is a molecule in the form of a double helix - aking an organism. All processes in the body or extracting DNA because they are squishy t times the amount of DNA as other fruits (they olate. d? s of the strawberries, releasing the DNA. the DNA. It also helps to keep the proteins t precipitate in the alcohol along with the DNA. e dissolved), they clump together and become ore, it makes the DNA strands clump together













Strawberry DNA E Step by Step Guid Corporate Social Responsibility Proprietary & Confidential

Extraction Activity de The world leader in serving science

Lab Safety Guidelines 1. Remember to conduct yourself in a responsible manner at all times 2. Review all safety information and procedures before beginning any activity or investigation. 3. Never eat, drink, chew gum or touch your eyes while working in the lab. 4. Keep your work area clean and neat. 5. Always make sure your instructor is present when you are working in the lab. 6. Never remove chemicals or equipment from the lab area. 7. Always wear ANSI Z87.1 approved chemical splash goggles when you are conducting experiments. 8. Be aware of the location of all safety equipment, including eye-wash stations, deluge showers, fire extinguishers and safety blankets. 9. When laboratory aprons are required, be sure to keep your apron tied throughout the entire activity. 2 Proprietary & Confidential



List of Materials, 1 Material Bottle of dish soap Container of salt 15 mL Conical Tube 5ml disposable pipette* Isopropol alcohol (500 mL) Sharpie Clear Plastic Cups 3 Proprietary & Confidential

Qty 1 1 2 1 1 1 2

List of Materials, 2 Material Basket of Strawberries Coffee filters* Roll of paper towels Small cups (Dixie cups)* scissors Ziplock bags* Water (500 mL) 4 Proprietary & Confidential

Qty 1 1 1 1 1 1 1

Table Set Up Volunteers sta SOAP SALT Students stan 5 Proprietary & Confidential

and on this side IPA water SOAP SALT nd on this side

Preparation Prior to Activity With Dispense 10 mL of water into one of the 15ml conicals. Volume ac 1 not too important. Dispense 5 mL of alcohol into one of the 15ml conicals. Volume ac not too important. Quarter the strawberries using the scissors (4 pieces) and place on 2 quarter of strawberry into separate ziplock bags. Remove stem fro strawberry. Prepare kits for Students • Ziplock bag containing strawberry piece • Conical containing 10 mls of water • Empty 15 mL conical 3 • 5 ml Disposable pipette bulb • Small cup • Coffee filter • Student Instructions • Paper towel 6 Proprietary & Confidential

h Students 10 mL 10 mL ccuracy is ccuracy is ne om

Student Activity Instructions Hand out one kit to each group Prepare Cell Lysis buffer • Add a generous pinch of salt to 15 mL conical containing water • Dissolve salt by inverting conical 10-15x • Add 10 drops of soap using 5 mL 1 disposable pipette • Carefully and slowly invert conical to dissolve thoroughly, be careful not to create bubbles Question 1: Why is detergent good at dissolving grease? What might the detergent be doing to the cells? 7 Proprietary & Confidential

Avoid Bubbles Slowly

Student Activity Instructions Prepare strawberries for DNA extraction • Mash the strawberry inside of the ziplock bag until it is a paste, use your 2 fists. • Pour the cell lysis buffer into the ziplock bag, pair with partner to do this • Gently mix the strawberry and lysis buffer together for 1-2 minutes. Gen 8 Proprietary & Confidential

tly

Student Activity Instructions Separate DNA from Proteins • Place coffee filter on top of Dixie cup • Slowly pour the mixture into the funnel shaped filter and allow the solution to drip through into the cup. Do not squeeze the filter as it may allow 3 proteins to also drip through • Once the solution has stopped dripping, remove the filter and place on paper towel. Discard into trash. • Add the solution in the Dixie cup to the 15 mL conical. Pinch both sides of cup to facilitate pouring. 9 Proprietary & Confidential

Reuse conical with lysis buffer

Student Activity Instructions ?m Precipitate DNA in Alcohol solution • Determine the total recovered volume from strawberry mixture in 15 mL conical by visual inspection 4 • Add an equal volume of Isopropyl alcohol slowly along the side of the 15 ml conical so that it layers on top of the strawberry mixture • Do not mix or invert the conical after adding alcohol Do not mix 10 Proprietary & Confidential

mL x

Student Activity Instructions Watch as the DNA comes out of solution! • The DNA will start to form on the interface between the strawberry 4 mixture and the alcohol. It will look like little bubbles on fine white stringy snot. Question 2: Why are we able to see the DNA after we add alcohol? 11 Proprietary & Confidential



How does this work!? • What does DNA stand for? • DNA stands for deoxyribonucleic acid. DNA two spirals twisting around each other. • What does DNA do? • DNA is the genetic instruction manual for ma are controlled by DNA. • Why do we use Strawberries? • Ripe strawberries are an excellent source fo and most important, strawberries have eight are octoploid), so there is a lot of DNA to iso • Why do we use the dishwashing liquid • The dishwashing liquid bursts open the cells • What does the salt do? • Helps to remove proteins that are bound to dissolved in the aqueous layer so they don’t • Why does alcohol clump the DNA? • When molecules are insoluble (unable to be visible. DNA is not soluble in alcohol; therefo and become visible to the naked eye. 12 Proprietary & Confidential

A is a molecule in the form of a double helix - aking an organism. All processes in the body or extracting DNA because they are squishy t times the amount of DNA as other fruits (they olate. d? s of the strawberries, releasing the DNA. the DNA. It also helps to keep the proteins t precipitate in the alcohol along with the DNA. e dissolved), they clump together and become ore, it makes the DNA strands clump together

Chemistry of Slime Corporate Social Responsibility Proprietary & Confidential

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