Chief, Department of Entomology MAJ Silas A. Davidson Deputy Department Chief CPT Katie Poole-Smith Executive Admin Assistant Quality Administrator (QA) vacant Nantavadee Suwanabun Admin Assistant Secretary Tanukorn Rumakom Yanischa Ketmunin สถาบนั วจิ ัยวทิ ยาศาสตรก ารแพทยท หาร Malaria Research, NRC Research Research Support Kevin Kobylinski, PhD ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES Vector Biology and Controlรายงานประจาํ ป 2561 91 ANNUAL REPORT 2018Diagnostic and Reemerging DiseasesMalariologyInsectary Mite and Rodent Support Alongkot Ponlawat, PhD Ratree Takhampunya, PhD Ratawan Ubalee, PhD Siriporn Phasomkusolsil, PhD Surachai Leepitakrat Medical Research Technologist Medical Research Technologist Medical Research Technologist Medical Research Technologist Medical Research Technologist Thanyalak Fansiri, PhD Achareeya korkusol Vichit Phunkitchar Kanchana Pantuwatana Taweesak Monkanna Patcharee Khongtak Amnart Kayha Orawan Thaveesuk Natthanun Auysawadi Boonsong Jaichapor Sasikanya Taloengsuk Waranya Buadok Arissara Pongsiri Nitma Chanarat Suparat Chairuksa Technician Insectary Technician Wachiraphan Chittham Yossasin Kertmanee Opas Thachin Nattaphol Pathawong Artharee Rungrojn Nattapat Rochanarutaiprida Supakit Wanasith Jaruwan Tawong Medical Research Technician Molecular Biologist Nantaporn Monkanna Udom Kijchalao Sommai Promstaporn Scrub Typhus Model Bousaraporn Tippayachai Piyada Charoensinphon, PhD Sakon Khaosanorh Somsak Tiangtrong Leptospirosis Model Medical Research Technologist Panadda Krairojananan, PhD Sirima Wongwairot Chawin Limsuwan Medical Research Technologist Total Personnel = 45 Kasima Wasuworawong Military = 2, FSN = 13, CA = 29, NRC = 1 As of 30 September 2018
Chief, Department of Immunology and Medicine COL Norman C. Waters, PhD สถาบันวิจัยวทิ ยาศาสตรก ารแพทยท หารDeputy Department ChiefGeneral Support COL Mark M. Fukuda, MD ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCESAdministrative Assistant Senior Clinical Study Physician Medical Research Aide Secretary Prapatsorn Ngaowichit Data Manager รายงานประจาํ ป 2561 92 ANNUAL REPORT 2018Michele D. Spring, MD Vacant Moocda Wongwiwat Nucharee Thongsen Chief, Clinical Trials Deputy Program Manager-DMAP Chief, Laboratory Operations MAJ Mariusz Wojnarski, MD LCDR Nicholas J. Martin, PhD CPT Brian A. Vesely, PhD Field Operations, Thailand Field Laboratory Field Operations, Cambodia Pharmacology and Bioanalytical Molecular Parasitology Laboratory Laboratory Section Chief Field Team Leader/Laboratory National Malaria Center Krisada Jongsakul, MD QA Cambodia Field Team Section Chief Section Chief Pattaraporn Vanachayangkul, PhD Panita Gosi, PhD Clinical Research Associates Worachet Kuntawunginn* Section Chief and Principal Investigator Nichapat U-Thaimongkol Chanthap Lon, MD, PhD Technicians Technicians Nillawan Buathong Lab Technicians Chanikarn Kodchakorn Chaiyaporn Chaisatit Sabaithip Sriwichai Chadin Thongpiam Clinical Research Physician Piyaporn Sai-ngam Vacant Chaiyawat Mathavarat Darapiseth Sea, MD, PhD Winita Ta-aksorn Vacant Kingkan Pidtana Kittijarankon Phontham Investigators Admin. (cont.) Montri Arsanok Somethy Sok, MD Thida San Parat Boonyarangka Saowaluk Wongarunkochakorn Ton Samfanna Malaria Culture and In Vitro Immunology and Vaccinology Drug Sensitivity Lab Laboratory Clinical Research Vandy Cheng Section Chief Investigator Coordinators (CRCs) Nonlawat Boonyalai, PhD Sathit Pichyangkul, PhD Chandara Sok Lab Technicians Technicians Technicians Chantida Praditpol Amporn Limsalakpetch Pheaktra Oung Bunly You Chatchadaporn Thamnurak Kirakarn Kirativanich Kosol Yongvanichit Samon Nou Kheangheng Thay Watcharintorn Fagnark Utaiwan Srichairatanakul Sohei Hom Lychhea Huot Soklyda Chann Mok My Sokna Ly Nareth Kong Phyra Phon Administrators Samel Buth Chantha Keo Sarath Nuom Rangsey R. Mok Soch Va Sea Em Vannak Pheap Sinin Cheam Vy Dav Total Personnel = 70 Military = 5, FSN = 15, CoAg = 17, HJF = 1, Sokha Yos Yom You Cambodia CoAg = 32 Sokhanny Yos As 30 of September 2018 Sokmeng Ry Data & IT Team *Holds multiple duties Sophea Yim Pheakdey Yan Somnang Iem
Chief, Department of Retrovirology COL Robert J. O’Connell Department Science Manager Deputy Department Chief Program Management Assistant Alexandra Schuetz*, PhD (Vacant) Suda Wilairatanaporn Secretary Jantana Uthesanan Director, Clinical Research Division Program Manager, Director, Laboratory Division Tanyaporn Wansom*, MD Research Support Section Head Deputy CAP Laboratory Director Robert A. Morgenthaler Siriwat Akapirat*, PhD สถาบนั วจิ ัยวทิ ยาศาสตรก ารแพทยท หารCOG, HeadECHO Medical ResearchIT Operation ManagerProgram CoordinatorHI, HeadCI Head, Senior Scientist Tanyaporn Wansom*, MD Physician Matthew D. Johnston Vivalya Khongkhakul CPT Thomas A. Musich Alexandra Schuetz, PhD ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES COG Senior Clinical Trials Suchai Kitsiripornchai, MD Lead IT Developer Administrative & HI, Medical Research CI, Laboratory รายงานประจาํ ป 2561 93 ANNUAL REPORT 2018Specialist Krittanandh Kraiwuttinandh Logistics Coordinator Technologist Supervisor Senior Investigator Nampueng Churikanont Somchai Sriplienchan, MD IT Support Nitit Isravudhakul Somsak Chantakulkij Yuwadee Phuang-Ngern Specialist Sirinan Madnote Weerawan Chuenarom COG Clinical Trials Specialist CE Nurse Coordinator Tossapol Ounhapattana Procurement Specialist Saowanit Getchalarat Patchara Charuthamrong Rungnapa Muanyart Jiraporn Puangkaew CI, Laboratory Nongluck Sangnoi HVRC IT Support Surawach Rittiroongrad Deputy Supervisor CE Nurse Technician Logistics Specialist Surat Jongrakthaitae QA Section Thippawan Yamkram Parnu Thongboonlua NHP Head QA Supervisor Wannapha Kaeratiswetanun Nutthapol Pramual Denise Hsu, MD, PhD Medical Research Technologist Sopana Chatnilbandhu Suchada Sukhumvittaya Recruiter/Educator NHP Laboratory Manager Nipattra Tragonlugsana QA Specialist Sophisuda Wiboonphan Decha Silsorn Boot Keawboon Benjapar Vesamavibool Chayada Sajjaweerawan Chettha Plainao NHP Medical Research Putita Saetun QA Coordinator Technologist Ajchariyarat Sangdara RN Laboratory Manager Sujitra Kujareevanich Yanin Kuncharin Supervisor, CAP Lab Dutsadee Inthawong Thunyasuta Prasit Jumpol Sopanaporn (ARCL/MOL) Puangmalee Buapunth Rapee Trichavaroj SPL, Specimen Processing Attapon Triampon and Archiving Manager Bessara Nuntapinit RN Nurse/Pharmacist Rojchana Piyabanharn SPL, Specimen Processing SPL, Specimen Archive CAP Lab SeniorMedical Supervisor Data Manager Research Technologist Research Assistant Chalatip Singhachuta Phiromrat Rakyat Anant Phramtong Pornchanok Panjapornsuk Total Personnel = 77 SPL Laborarory Technician SPL, Senior Laboratory SPL, Repository Technician CAP Laboratory, Military = 2, FSN = 5, HMJF = 6, PRTR = 64 Panjaree Ruangjan Technician Kajornsak Chaiteamwong Medical Research Anake Nuchwong As of 30 September2018 Surasit Inprakong Nitipan Klamyusuk Technologist *Dual-Hatted Sukanya Lucksanawong Anawat Phramtong Nantana Tantibul Pannadda Kruacharoen SPL, Support Technician Sarawoot Mongkolpun Bhubate Tongchanakarn Wayut Sajjaweerawan Thiti In-ngarm Paramate Promnarate Suwanee Techapitaksat SPL, Data Entry Vatcharain Assawadarachai Worakarn Wongchankham Pongstron Peachyai Panupat Nadee Karnjana Techwongtham Witchuda Khamthai Chaichana Chuendoung
Chief, Department of Veterinary Medicine & Attending Veterinarian LTC Luis A. Lugo สถาบันวิจัยวทิ ยาศาสตรก ารแพทยท หารDeputy Department Chief &Chief, Divisions of Comparative Pathology & Chief, Research and Laboratory Animal Medicine Veterinary Medical Research ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCESLTC Matthew D. Wegner Rawiwan Im-Erbsin, DVM รายงานประจาํ ป 2561 94 ANNUAL REPORT 2018 Clinical Veterinarian, Program Management Supervisory NCOIC/Program Manager Administrative Section QC, Training, GLP Medical Research Kesara Chumpolkulwong, DVM SSG Carrie L. Josephson Admin Assistant Wallapa Thajeen Supervisor Scientist IACUC/Secretary Angwara Arinhamapan Clinical Veterinarian Charin Kheowcharas Piyanate Sunyakumthorn, PhD Vacant, DVM Administrative Associate Ganokwan Chaksurak Quality Control Assistant Environmental Enrichment Launderer Boonhome Meepuak Surayuth Seegaewin Coordinator Launderer Sunisa Kaewwaen Siwakorn Sirisrisopa Medical Research Technologist Environmental Enrichment Manutsanun Inthawong Coordinator Technician Sakda Wongsawanonkul Supervisor Supervisor Supervisor Supervisor Clinical Lab Supervisor Vet Care Section Support Section Non-human Primate Section Small Animal Section Laksanee Inamnuay Mana Saithasao Chardchai Burom Natthasorn Komcharoen Anchalee Tungtaeng Veterinary Technicians Support Technicians , Caretakers, Technologists, Technicians Medical Research Alongkorn Hanrujirakomjorn Technologist Support Section NHP Section Small Animal Section Noppon Popruk Nuttawat Wongpim Sawaeng Sripakdee Chakkapat Detpattanan Dejmongkol Onchompoo Khrongsak Saengpha Sarayuth Chienrum Sornsawan Kommee Siriphong Prompim Rachata Jecksaeng Phakorn Wilaisri Thanaphon Rattanathan Yongyut Kongkaew Medical Technologist Sonchai Jansuwan and Histotechnologist Paitoon Hintong Siwadol Sammano Amnat Andang Sujitra Tayamun Sakchai Lerdmontee Chaisit Pornkhunviwat Total Personnel = 46 Vacant Manas Kaewsurind Military = 3, FSN = 9 CoAg = 34 Wuthichai Puenchompu Sitthidech Hanphongsak As of 30 September 2018 Yosswat Kumhun Vacant Vacant
Consultant in Virology/Serology Director, Virology Laboratory Chief, Department of Virology Clinical Research Coordinator Deputy Department Chief Khunying Ananda Nisalak, MD and Epidemiological Services COL Louis R. Macareo, MD Thidarat Intararit COL Alden L. Weg, MD LTC Stefan Fernandez, PhD Regional Clinical Investigator Administrative and Suttiman Wattanasrirote Kamphaeng Phet-AFRIMS John Mark S. Velasco, MD Molecular Logistics Support Wanatsanan Sitkittitad Virology Research Unit Chonticha Klungthong, PhD Prapanrat Kivwongngam (KAVRU) Clinical Investigator Nualanong Akaraputtiporn Kanyakorn Rattanakorn Darunee Buddhari, MD Kathryn B. Anderson, MD, PhD Kittinun Hussem Russama Jittawisutthikul Piyawan Chinnawirotpisan, PhD Ubon Srangsrok Laboratory Section Database Administrator Yuwadee Sae-Yong Forradee Nuchsongsin Benjawan Khuntirat, PhD Tippa Wongstitwilairoong Prinyada Rodpradit Apinop Surayan Thipwipha Phonpakobsin Quality Systems Unit Wallika Kulthongkam Bioinformatics Consultant Wudtichai Manasatienkij Supaporn Sakaekhao Nawarat Charoensri Wichien Sa-Nguansuk Wiriya Rutvisuttinunt, PhD Yongyuth Poolpanichupatam Kanyarat Phutthasophit Philippines AFRIMS Wipa Chawachalasai Khajohn Joonlasak Virology Research Unit Wiangchai Watcharanirun Nantawan Kulchon สถาบนั วจิ ัยวทิ ยาศาสตรก ารแพทยท หารRaveewan Siripokasupkul Virology/Serology Project Management (PAVRU) Study Coordinators/Nurses Butsaya Thaisomboonsuk, PhD ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCESChitchai HemachudhaMaria Theresa P. Alera, MDRungkarn Hangsuwan Chaleaw Saengchan Viral Serologyรายงานประจาํ ป 2561 95 ANNUAL REPORT 2018Information SystemDeputy Head Dararat Kanjana Duangrat Mongkolsirichaikul Management Paula Corazon S. Diones, MD Chuthatip Mahayos Taweewun Hunsawong, PhD Prapapun Ong-Ajchaowlerd Tipawan Thipwong Administrative Log Administrative Support/QC Worrawut Leelanimit Support Data Management Rattiya Wannawong Rungarun Suthangkornkul Jittima Khiannukul Frances Dominique Y. Villano Rungkarn Kaenman Kanittha Sirikajornpan Wicha Panyalikhit Angkana Huang Margie Meca Parado Wanchai Inpho Kamonthip Rungrojcharoenkit Alberto Amora Belleza Kedsara Tayong Specimen Processing Pannarat Cheukanobon Prachakkra Panthusiri Danny C. Obidas Noppol Sirichai Viral Culture Nino J. Embalsado Chuanpis Ajariyakhajorn, DVM Rewadee Klinmala Raymundo P. Sanchez Supaporn Kaewpongsri Parinya Kruacharoen Thongchai Khiankaew Edlyn A. Achay Chutithorn Tawilert Naphat Yimthin Jewernest C. Casquejo Winai Kaneechit Wonlana Theerapolumpun Lab and Clinical Jindarat Lohachanakul Wimonsri Tinrum Catherine B. Lago Susie G. Leonardia URI Research Assistant Maria Theresa G. Valderama Total Personnel = 95 Klanarong Wongsaen Maria Teresa Despacio Michael Ian T. Cuizon Military = 3, FSN = 20, CoAg = 65, Contract = 7 Methee Gomootsukavadee Maricar C. Vismanos As of 30 September 2018 Angeli Mae B. Reyes Jaspher H. Baliguat Jhufelyn Lou T. Inso
สถาบันวจิ ยั วทิ ยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES USAMD-AFRIMS Department of Administration Information Management Division Mission: The Information Management Division (IMD) at USAMD-AFRIMS provides information technological, library, medical audio visual and language services to USAMD-AFRIMS personnel. It does this with a full-time staff of one (1) US Military, one (1) GS Civilian, six (6) Foreign Service Nationals, and nine (9) Cooperative Agreement local nationals. Discuss Significant Activities Related to Organizational Mission: USAMD-AFRIMS IMD continues to provide exceptional administrative and technical support to all organizational areas in the organization’s effort to defeat infection. IMD enables the organization to tell its story to a global audience via scientific posters, publications, photography and videography, library services, research support, as well as WRAIR social media. The division also enables network connectivity, cybersecurity, telecommunications, and lifecycle support for all areas, to include Thailand, Cambodia, Nepal, and the Philippines. Constraints/Problems Encountered: Coordination with DISA and WRAIR IMD continue to present challenges due to inherent time zone differences; limited network administration capabilities hinder ability to administer MIL network in timely manner, resulting in avoidable down times. In addition, AFRIMS and WRAIR Security, coordinating with AFRIMS and WRAIR IMD, continue to clarify interpretation of Department of State background investigations as they relate to Department of Defense requirements for Local National network access. Solutions Devised: 1. Information Technology 1.1. AFRIMS Organizational Network • To clarify Local National network access requirements, WRAIR Personnel Security continued to consult with Army G2 for official interpretation of background investigation requirements. • Deployed Army Golden Master (AGM) Windows 10 to 40% of computers in AFRIMS network • Implemented Work Folder feature allowing end users to store work files in their computers and the feature will automatically replicates the files to data center servers • Purchased and configured: storage server with 60 TB space; Zmanda, backup server, to backup shared files to storage server; Nagios XI, infrastructure monitoring software to monitor servers, switches, and workstations • Purchased and configured: Cisco ESA, mail security gateway, to detect and block email-borne threats such as malware, spam and phishing attempts รายงานประจําป 2561 96 ANNUAL REPORT 2018
สถาบันวจิ ัยวิทยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES • Purchased and configured: Cisco Identity Services Engine (ISE) is the network administration product that can centralize security and access policies for the endpoint devices such as switches. Now all access to switches in AFRIMS are centralized and managed by ISE. • Purchased and configured: Varonis, a security software platform to track, visualize, analyze and protect AFRIMS Personally Identifiable Information and Protected Health Information • Enforced Host Based Security System (HBSS) feature in McAfee on desktop to minimize the risk 1.2. Army.Mil Network • 100% compliance with Windows 10 1709 update established in February 2018 • Established mail.mil access on mobile devices • Continued after-hours coordination with DISA and WRAIR IMD to address network issues • IT Specialist pursuing Cisco Certified Network Associate certification in order to gain network administrative rights to .Mil network 2. Information Resources Center 2.1. Library • Assisted patrons via visitations and correspondence 529 times; catalogued 372 books, videos, and journals; acquired 8 books, 31 journal subscriptions, 4,000+ online journals and electronic books • Maintained a circulation of 2,638 books, journals, and videotapes; conducted 541 inter-library loans; conducted 193 literary database searches from within WRAIR and AFRIMS; conducted updates on 4,715 electronic resources; analyzed 2,487 AFRIMS published articles 2.2. Medical Audio-Visual Section (MAVS) • In FY18, MAVS printed 248 photographs, produced 17 Department of the Army and 79 civilian photos, covered 29 events, edited 151 photographs, and produced 3 videos. • Organized and assisted with production of RTA-US AFRIMS Organizational Video, last updated in 2008 • Produced 2,042 graphics and 123 posters, as well as laminated 792 products and bound 211 books • Prepared the conference room for meetings and trainings 280 times 2.3. Language Instruction • Conducted English classes for 200 students: The classes are Easy basic English (everyone can learn), Email writing, English class for IT staff, English class for special purposes, English for drivers, Essential vocabulary for ALAT, General English 1-6, Grammar and Vocabulary for TOEIC test, Remedial English 1-2, Telephoning skills, English for customer care, English through songs, Boost your TOEIC score • Published English Tips newsletter (52 issues), circulated weekly to 233 subscribers • Provided AFRIMS English recruitment test to 30 AFRIMS candidates รายงานประจําป 2561 97 ANNUAL REPORT 2018
สถาบันวิจัยวทิ ยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES • Conducted Thai classes for 20 American students. The classes encompassed Thai culture and language in all skills with 3 different levels including reading. • Started Thai Culture and Holiday newsletter (9 issues), circulated on Thai holidays to 36 non-Thai members New Missions Added: Ms. Kaewkarn Noonpakdee joined AFRIMS and transitioned to the Information Resources Center team as the Thai Language Instructor, a welcome complement to our English Language Instructor. Old Missions Discontinued: N/A Outcomes: USAMD-AFRIMS IMD team continuously leverages technology and personnel to improve support to the organization’s mission. Laboratory Operations Division Discuss Significant Activities Related to Organizational Mission: The Laboratory Operations Division provides safety and biosafety guidance to USAMD- AFRIMS by interpreting and implementing policies from higher headquarters, local and federal laws to assure safety and biosafety within the organization. Semi-annual occupational health and safety inspections were completed in all laboratories within Thailand, Cambodia, the Philippines and Nepal. Annual Occupational Health and Safety training was completed at all sites and for incoming personnel as well as visiting personnel. Laboratory Operations has two of the three AFRIMS National Sanitation Foundation (NSF) certifiers for certifying approximately 100 biosafety cabinets in seven different locations. Laboratory Operations manages the AFRIMS Freezer Farm research repository that houses more than one million research samples. Laboratory Operations provided training to researchers, students, doctors and nurses at universities, the Thai NIH, and biosafety workshops on safety and biosafety topics upon request. Hands on BSC certification training was provided to two certifiers from the International Organization for Migration (IOM) at USAMD-AFRIMS upon the request of the IOM. The Joint Safety office at WRAIR completed a site assistance visit of AFRIMS facilities in Thailand, Nepal and Cambodia. Several AFRIMS practices were cited as best practices for AFRIMS and WRAIR. Laboratory Operations staff participate in the Bureau of Laboratory Quality Standards, Department of Medical Sciences, Ministry of Public Health, Thailand, committee for the establishment of guidelines, criteria and standards for the Pathogens and Animal Toxins Act. Occupational Health conducted ergonomic training and assessment of laboratory and office staff to improve the workspaces and minimize the risk of injury to AFRIMS employees. Constraints/Problems Encountered: • The Ministry of Public Health in Thailand revised their Infectious Agents and Toxins (IAT) policy and submission guidance requiring an annual fee from each submitting agency. • Reduction of staff due to the retirement of one Foreign Service National (FSN) employee primarily responsible for IAT submission and TSMP management รายงานประจําป 2561 98 ANNUAL REPORT 2018
สถาบนั วจิ ัยวิทยาศาสตรก ารแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES • When GEIS funding is delayed, Laboratory Operations lacks funds to complete tasks such as managing the BSL-3 laboratory. Solutions Devised: • IAT submission is a combined US and RTA-AFRIMS report where the RTA pays the registration fees and provide the required clearances for the new process. • IAT and TSMP duties are assumed by the senior Biosafety Specialist until the IAT Program Manager position is filled. • The BSL-3 maintenance is funded primarily by GEIS and when funds are not received at the beginning of the fiscal year, we must borrow funds to maintain the BSL-3. New Missions Added: None Old Missions Discontinued: None Outcomes: AFRIMS continues to conduct quality research in a safe and low risk environment. Research Support Services Division Mission: The mission of the Research Support Services Division (RSSD) is to support the USAMD- AFRIMS research departments on research activities and to ensure that USAMD-AFRIMS research activities comply with US, DoD and local regulations and WRAIR policies. Discuss Significant Activities Related to Organizational Mission: The newly-established RSSD is composed of the Human Subjects Protection Office (HSPO), Quality Unit (QU), a Technology Transfer Specialist, Research Support Administrators who support scientific review and publication clearance process, a Biostatistician and a Training Coordinator. The major activities of the HSPO are to interpret and apply regulations, polices, and guidelines; develop SOPs and policies; perform protocol/report pre-review to ensure compliance with WRAIR/AFRIMS submission and WRAIR requirements; and provide review and determination for NHSR/exempted protocols. The QU 1) reviews, tracks, distributes, archives, and maintains SOP database, 2) provides monitoring and audit capabilities, and 3) provides research pharmacy support. The Technology Transfer Specialist assists USAMD-AFRIMS with business agreements and potential intellectual property rights/issues. The Research Support Administrators provide support for scientific review and publication clearance process and protocol submission. The Biostatistician provides statistical consultation and services to researchers for human, animal, and laboratory studies. The Training Coordinator coordinates in- house trainings and maintains a database of trainings for all AFRIMS employees. Constraints/Problems Encountered: • Conflicts of US and local regulations/requirements on human subject protection • Lack of coordination between the Technology Transfer Specialist, the HSPO, the Training Coordinator, and the Research Support Administrators to create visibility on protocol scientific review and submission, publication, and visitor clearance to ensure all relating research activities are to be done appropriately รายงานประจาํ ป 2561 99 ANNUAL REPORT 2018
สถาบนั วิจัยวิทยาศาสตรการแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES • AFRIMS-wide SOP and policy training is not as robust in AFRIMS’s outlying sites in Cambodia, Nepal, and the Philippines. • Being unable to access WRAIR website reduces awareness of the RSS personnel to keep up with WRAIR SOPs and policies. • Lack of personnel to support the Director of HSPO in the scope of human protection activities since she has been assigned to have broader responsibilities to oversee RSSD Solutions Devised: • Participate in local conferences and research for local regulations/requirements in order to identify the conflicts, ensure local authorities aware of US requirements and work with them to ensure compliance with US requirements • Appropriate visibility in protocol scientific review and submission, publication, and visitor clearance has been given to the Technology Transfer Specialist and the HSPO. • AFRIMS-wide SOP and policy training is planned for AFRIMS staff in outlying sites for 2019. • Request WRAIR POC for regular updates on new/revised SOPs and policies • Request support from WRAIR HSPB to define human subject protection activities to avoid duplication of protocol and report review at AFRIMS and WRAIR level New Missions Added: • Revise AFRIMS SOPs and policies to comply with the new Common Rules (45 CRF 46) effective 21 January 2019, DoD regulation and WRAIR SOPs and policies • Enhance local authorities’ awareness of the new requirement per 45 CFR 46 Old Missions Discontinued: N/A Outcomes: • AFRIMS-wide SOP and policy training was provided to key overseas staff from Nepal and Cambodia in Bangkok adjacent to the GCP training in January 2019. • The WRAIR HSPB and AFRIMS HSPO have conducted monthly teleconference to update policies and SOPs. Routine communication between AFRIMS Research Support and WRAIR Public Affairs Office and weekly Agreements Review Committee meeting has been going well. Connection between AFRIMS and WRAIR QU is to be improved. • The AFRIMS HSPO are working closely with WRAIR HSPB on work assignment and preparing for the changes due to the new 45 CFR 46. • Connection between each area in RSSD has been established so the RSSD can provide services to USAMD-AFRIMS research departments early on in protocol development and can assure USAMD-AFRIMS compliance with DoD and WRAIR requirement in all research related activities. รายงานประจําป 2561 100 ANNUAL REPORT 2018
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FFYY1199 DDooDD ffuunnddiinngg wwaass ffuullllyy ffuunnddeedd bbyy CCoonnggrreessss pprriioorr ttoo tthhee ffiissccaall yyeeaarr ssttaarrtt.. AAFFRRIIMMSS hhaass yyeett ttoo rreecceeiivvee aannyy GGEEIISS ffuunnddiinngg aass ooff 2200 FFeebbrruuaarryy 22001199.. •• SSuubb--CCaasshhiieerr RReessttrriiccttiioonnss –– UUSSAAMMDD--AAFFRRIIMMSS iiss aauutthhoorriizzeedd aa ssuubb--ccaasshhiieerr ffrroomm tthhee UUSS EEmmbbaassssyy BBaannggkkookk.. IInn FFYY1199,, iinnssppeeccttiioonn rreessuulltteedd iinn tthhee ccaasshh ooppeerraattiioonnss nnoott aaddhheerriinngg ttoo tthhee UUSS SSttaattee DDeeppaarrttmmeenntt FFoorreeiiggnn AAffffaaiirrss MMaannuuaall.. TThhiiss hhaass ppuutt mmoorree ccoonnssttrraaiinntt oonn tthhee pprrooccuurreemmeenntt sseeccttiioonn ooff UUSSAAMMDD--AAFFRRIIMMSS.. •• DDiissbbuurrsseemmeenntt RRaatteess ffoorr UUSSAAMMDD--AAFFRRIIMMSS –– AAnnyy ttrraannssaaccttiioonn tthhaatt iiss eexxeeccuutteedd tthhrroouugghh tthhee UUSS EEmmbbaassssyy,, BBaannggkkookk wwiillll hhaavvee aa ttiimmee ddeellaayy iinn tthhee ddiissbbuurrsseemmeenntt.. TThheessee ttrraannssaaccttiioonnss ccoonnssiisstt ooff aallll FFoorreeiiggnn SSeerrvviiccee NNaattiioonnaall ssaallaarryy,, TTDDYYss tthhaatt aarree wwiitthhiinn tthhee UUSS SSttaattee DDeeppaarrttmmeenntt ttrraavveell ssyysstteemm ((EE22)) aanndd aallll pprrooccuurreemmeenntt iitteemmss ssuubbmmiitttteedd tthhrroouugghh tthhee UUSS EEmmbbaassssyy,, BBaannggkkookk aanndd KKaatthhmmaanndduu GGeenneerraall SSeerrvviiccee OOffffiiccee ooffffiicceess.. UUSSAAMMDD mmuusstt iinnppuutt aa mmaannuuaall oobblliiggaattiioonn iinn GGFFEEBBSS ffoorr aallll ooff tthheessee ttrraannssaaccttiioonnss.. UUSS EEmmbbaassssyy wwiillll ssuubbmmiitt rreeiimmbbuurrsseemmeenntt vvoouucchheerr tthhrroouugghh tthhee GGlloobbaall FFiinnaanncciiaall SSeerrvviicceess ttoo tthhee DDeeffeennssee FFiinnaannccee aanndd AAccccoouunnttiinngg ooffffiicceess.. TThhee DDFFAASS ooffffiicceess wwiillll sseenndd tthheessee uunnmmaattcchheedd ddiissbbuurrsseemmeennttss ttoo WWRRAAIIRR RRMM ttoo mmaattcchh aaggaaiinnsstt tthhee mmaannuuaall oobblliiggaattiioonnss.. TThhiiss ccyyccllee ttaakkeess aa mmiinniimmuumm ooff 44 mmoonntthhss ttoo ccoommpplleettee ttoo sshhooww aa ddiissbbuurrsseemmeenntt iinn GGFFEEBBSS.. SSoolluuttiioonnss DDeevviisseedd:: •• DDeeppaarrttmmeennttss aarree aabbllee ttoo ddeellaayy ssoommee pprroojjeeccttss iinn rreellaattiioonn ttoo ffuunnddss aarrrriivviinngg llaattee.. IInn ssoommee ccaasseess,, ffuunnddss ffrroomm ootthheerr pprroojjeeccttss mmuusstt bbee uusseedd ttoo mmaaiinnttaaiinn mmiissssiioonn rreeaaddiinneessss aanndd aaccccoommpplliisshhmmeenntt.. •• SSoommee ddeeppaarrttmmeenntt cchhiieeffss hhaavvee hhaadd ttoo ffiillee rreeiimmbbuurrsseemmeenntt ccllaaiimmss bbyy hhaavviinngg ttoo ppaayy ffoorr mmiissssiioonn rreellaatteedd ccoossttss dduuee ttoo pprrooccuurreemmeenntt uunnaabbllee ttoo pprroocceessss ppaarrttiiccuullaarr mmiissssiioonnss.. •• AAss lloonngg aass pprrooccuurreemmeenntt iiss tthhrroouugghh UUSS SSttaattee,, tthheerree wwiillll bbee aa llaagg iinn ddiissbbuurrsseemmeenntt bbeeccaauussee GGFFEEBBSS ddooeess nnoott ccoommmmuunniiccaattee wwiitthh UUSS SSttaattee DDeeppaarrttmmeenntt ffiinnaanncciiaall ssyysstteemmss.. AAFFRRIIMMSS iiss eexxpplloorriinngg aalltteerrnnaattiivvee pprrooccuurreemmeenntt ssttrraatteeggiieess.. NNeeww MMiissssiioonnss AAddddeedd:: NNoonnee OOlldd MMiissssiioonnss DDiissccoonnttiinnuueedd:: NNoonnee รายงานประจาํ ป 2561 101 ANNUAL REPORT 2018
สถาบันวิจัยวิทยาศาสตรการแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES Outcomes: USAMD-AFRIMS RM was able to close out all expiring funding in accordance with fiscal laws. Department of Logistics Mission: The mission of the Department of Logistics is to ensure that the USAMD-AFRIMS research departments, and other supported US Government agencies, as directed, receive required general and laboratory supplies, equipment, biomedical maintenance, facilities maintenance, transportation, glassware, shipping, property accountability, and life cycle management services in an efficient and cost effective manner. This logistics support extends to various field sites and laboratories located throughout the Kingdom of Thailand, Nepal, the Philippines, Cambodia, Bhutan, and in other countries in the INDOPACOM AOR as required. Discuss Significant Activities Related to Organizational Mission: USAMD-AFRIMS needed to shift programmatic procurement support requirements to DoD supporting elements IAW MOU-DoD & STATE CABLE 00030953, ISSUED 4 April 2011 and 18 August 2015 SAV the GSO conducted for the Office of the Procurement Executive. Strengthen partnerships and information sharing amongst DoD enablers thought the Pacific area of responsibility (AOR) such as: US Indo-Pacific Command (USINDOPACOM); US Army Pacific (USARPAC); Theater Lead Agent for Medical Materiel-Pacific (TLAMM-P); Defense Logistics Agency Pacific (DLA); US Army Medical Materiel Center-Korea (USAMMC-K); and the 6th Medical Logistics Management Center (6th MLMC) Constraints/Problems Encountered: • Significant reduction in support provided by the GSO office at the US Embassy Bangkok • Conducting facility planning and execution for the same FY Solutions Devised: • Hire a Facility Operations Specialist GS12 to create depth in the facilities section and serve as the full time chief of the facilities department • Develop a concept of support that outlines simplistic and economic processes and procedures to increase responsiveness while minimizing the complexity of sustainment • Increase the number of procurement agents to process requests • Increase the revolving credit limit of GPC New Missions Added: N/A Old Missions Discontinued: N/A รายงานประจาํ ป 2561 102 ANNUAL REPORT 2018
สถาบันวจิ ยั วทิ ยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES Outcomes: As AFRIMS continues to foster streamlining of requirements and develop a JIIM (joint, interagency, intergovernmental, and multinational) Concept of logistics support we anticipate a more responsive supply chain. Department of Enteric Diseases Mission: The mission of the Department of Enteric Diseases is to develop and evaluate interventions to diagnose, treat, and prevent diarrheal disease and other infectious diseases of military importance. Common, recurring threats to joint military force health protection and mission success remain diarrheal diseases and loss of effective treatment options from antibiotic/antimicrobial resistance. The department conducts disease surveillance studies in South and Southeast Asia to help identify disease patterns, incidence rates, and trends for Geographic Combatant Commander awareness, improve medical planning, and identify effective treatments. The department also contributes to the evaluation, development, and implementation of new diagnostic tests for these purposes. Discuss Significant Activities Related to Organizational Mission: 1. Diarrheal Diseases Surveillance in South and Southeast Asia 1.1. The department conducts diarrheal surveillance in Thailand: • Conducted a prospective case-control study at a local civilian hospital (Bumrungrad, Bangkok) to determine the etiology of travelers’ diarrhea and to describe development of post-infectious sequelae and chronic intestinal symptoms for up to one year in study subjects • Cobra Gold is an annual exercise between the Royal Thai military and INDOPACOM units conducted in multiple locations in Thailand. Surveillance for acute diarrhea in the 6,500 US military members participating in Cobra Gold was conducted in 2018. Distillation of prior Cobra Gold surveillance data into a practical medical annex reduced diarrheal disease from 8, to 4, to zero cases over the last three years. • A prospective prevalence study to determine intestinal parasitic infections among school children aged 3-12 years was conducted at small villages in the Kanchanaburi province, along the Thai-Myanmar border in rural Thailand, 2017-2018. A total of 661 students from 3 schools submitted stool samples for testing. Among the clinical important intestinal parasites, Giardia was most common (17%), while others were infrequently detected at or below 1%. Characterization of Giardia parasites and their drug susceptibility patterns will be useful information for epidemiology and treatment of Giardiasis in Thailand. 1.2. The department conducts diarrheal surveillance in Nepal: Initiated a traveler’s diarrhea study at the CIWEC (Canadian International Water and Energy Consultants) Travel Medicine Clinic, with two locations in Kathmandu and Pokhara, Nepal. This study is a part of the multicenter Global Traveler’s Diarrhea Study. Enrollment and surveillance of Western expats and travelers in-country suffering from diarrhea serve as a surrogate population for what joint military members may face if deployed to the region. รายงานประจาํ ป 2561 103 ANNUAL REPORT 2018
สถาบันวิจยั วทิ ยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 1.3. The department conducts diarrheal surveillance in Cambodia: A prospective case control study to describe acute diarrhea etiologic agents and antimicrobial resistance was initiated in July 2014 and surveillance has continued into 2018. Study volunteers are Cambodian children and adult military personnel at the Battambang Referral Hospital, SvayPor Health Center, and the Military R5 Hospital in Battambang, Cambodia. 2. Antibiotic and Antimicrobial Surveillance in Southeast Asia 2.1. The department conducts disease resistance surveillance in Thailand: • Phramongkutklao Military Hospital, located in Bangkok, is the major teaching and referral hospital for the 36 hospitals in the Royal Thai Army Medical Department. Currently, the project has characterized over 700 multidrug resistant bacteria isolated from patients. 37 isolates were sequenced at the Walter Reed Army Institute of Research Multidrug Resistant Organisms Repository and Surveillance Network (MRSN). • Queen Sirikit Naval Hospital is a premier healthcare facility for the Royal Thai Navy with a 1,000 bed capacity in Chonburi, Thailand. As part of their internal infection control program, the facility collects and monitors antibiotic and antimicrobial resistance. Over 600 bacterial isolates were shared with AFRIMS and the MRSN, and results identified unique resistance patterns never before reported in Thailand. • Surveillance of antibiotic resistant N. gonorrhoeae in adult patients seeking care at Phramongkutklao and other Thai military hospitals. To date 120 samples were collected (112 from Thai military) from Chonburi (1/120; 0.8%), Lampang (2/120; 1.7%), Chiang Rai (3/120; 2.5%), Lopburi (42/120; 35%), and Bangkok (72/120; 60%). Many of these were resistant to tetracycline (88/102; 86.3%), penicillin (91/102; 89.2%), and ciprofloxacin (96/102; 94.1%). 2.2. The department conducts disease resistance surveillance in the Philippines: The Armed Forces of the Philippines Medical Center is the major referral hospital for military service members and their dependents in the Philippines. Similar to the study being conducted at Phramongkutklao Hospital located in Bangkok, Thailand, bacterial isolates from patients are collected and characterized to assess multidrug antimicrobial resistance. To date, over 1,400 bacterial isolates have been characterized for the study. 3. Other Pathogen Surveillance Efforts in Southeast Asia Departmental assessments conducted in Thailand: 3.1. Nasal and nasopharyngeal swab samples were collected from influenza-like illness patients visiting 8 Royal Thai Army border hospitals in Chiang Rai, Chumporn, Kanchanaburi, Nakorn Phanom, Nan, Pattani, Sra Kaeo and Ubon Ratchathani provinces during March 2007 to February 2013 and tested by real-time RT-PCR to identify H1 and H3 subtypes. A total of 174 samples (103 of H1 and 71 of H3) were selected for influenza virus isolation by cell culture using MDCK cells. Whole genome sequencing was performed on 120 selected isolates (80 H1N1 and 40 H3N2) and these were submitted to GenBank as accession numbers KP637275-KP638234. 3.2. 6,627 serum samples collected from Royal Thai Army recruits in 2012 from all 77 provinces in Thailand were tested positive for the exposure to the following pathogens: 124 (2%) to Coxiella burnetti; 2,105 (32%) to scrub typhus; 1,176 (18%) to Leptospira spp; 296 (4.5%) to Spotted Fever group Rickettsia; and 339 (5.1%) to Rickettsia typhi (murine typhus). 3.3. 1,000 serum samples collected from Royal Thai Army recruits in 2012 from all 77 provinces were assessed for evidence of prior exposure to the following pathogens: 88.5% were positive for hepatitis A, 63.3% for herpes simplex virus 1, 7.4% for type 2, 47% human รายงานประจาํ ป 2561 104 ANNUAL REPORT 2018
สถาบันวจิ ัยวิทยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES papilloma virus, and 0.7% hepatitis C. The demographic information (age, educational level, marital status, occupation, province of birth and residence, and urban or rural location) was linked to antibody prevalence data, and this can be analyzed to monitor infection risks. 4. Diagnostic Platform Development and Novel Pathogen Testing 4.1. Surveillance of two emerging gastrointestinal pathogens Tropheryma whipplei (Whipple’s disease) and Escherichia albertii in acute diarrhea cases and controls using molecular methods. A total of 1,030 previously pathogen-negative samples (cases and controls) from travelers and children diarrhea surveillance studies from Nepal, Thailand, and Cambodia were evaluated for these pathogens using a real-time PCR assay, and 11 (1%) were positive for T. whipplei. Escherichai albertii prevalence was obtained by screening non-lactose fermenting Enteropathogenic E. coli isolates from this subset for lysP and mdh genes, both associated with E. albertii, and 18 E. albertii isolates were identified. 4.2. An evaluation of TaqMan Array Cards (TAC) on traveler’s diarrhea samples. This is a 384-well customized real-time PCR array card containing reagents to detect diarrhea pathogens, which was compared to conventional detection methods for these disease agents. Briefly, a comparative analysis showed that results obtained by TAC are similar to results obtained by conventional methods, demonstrating TAC as a faster, more efficient, and effective diagnostic tool for diarrhea etiologic agents. 4.3. Evaluation of xTAG Gastrointestinal Pathogen Panel (GPP) multiplexed nucleic acid test for the detection of diarrheal pathogens in stool samples. This platform can simultaneously detect and identify these pathogens: Campylobacter, Clostridium difficile, Escherichia coli O157, ETEC, STEC, Salmonella, Shigella, Vibrio cholera, Yersinia enterocolitica, adenovirus, norovirus, rotavirus A, Cryptosporidium, Entamoeba histolytica and Giardia). In comparison with conventional diagnostic tests, the xTAG GPP assay demonstrated increased sensitivity towards Campylobacter, Salmonella, norovirus GII, ETEC and shiga-toxin producing E. coli (STEC), with high sensitivity ranging from 97.6-100% and specificity ranging from 94-100%. 5. Diarrhea Countermeasure Development The department completed a placebo-controlled non-human primate (NHP) study of the ability of an internationally commercially available (but not yet evaluated by USFDA) cow colostrum (antibody) product (Travelan) for the prevention of diarrhea in a Shigella challenge model. Eight NHP received Travelan, and 4 received placebo prior to an oral challenge with viable Shigella flexneri. All 4 placebo animals displayed dysentery symptoms, compared to 2 of 8 animals who received the antibody product, demonstrating evidence for protection in this model. The way forward includes adaptation of this production method to create products specific for diarrhea causing bacteria in Southeast Asia for further testing. Constraints/Problems Encountered: • AFRIMS has a diverse workforce comprised of US military, GS civilians, US contractors, Foreign Service Nationals (GS equivalents), and foreign contractors. Funding sources are restricted to only certain staff categories, even though most staff are contributing members to funding agency projects. This creates workforce management challenges. • Protocol and regulatory approval processes to conduct simple surveillance efforts, independent of human subjects, are lengthy, complicated, and inconsistent. Interested collaborators often withdraw their support when they realize how long this process takes, and one cancelled a study mid-stream because we could not honor their reasonable deadlines. รายงานประจําป 2561 105 ANNUAL REPORT 2018
สถาบันวจิ ัยวิทยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES Solutions Devised: • Currently, funding diversity is mirrored by diversity of employee type. Maintenance of this balance requires careful planning, and maintenance of numerous types of employee types. • Careful management of approval processes is the best solution to minimize delays. To this end, AFRIMS scientists from around the region will participate in Good Clinical Practices training, and we have strengthened our Human Subjects Protection training as ways for our protocol creation to more closely conform to regulations. • The fusion of the former Enteric and Immunology/Medicine Departments into the new Department of Bacterial and Parasitic Diseases provided additional resources and synergies each lacked individually, addressing many of the challenges mentioned above. New Missions Added: None Old Missions Discontinued: NSTR Outcomes: • Serve as a regional Subject Matter Expert Exchange to collaborative scientists and medical professionals in Bhutan, Nepal, the Philippines, Cambodia, and Thailand for the pathogens and techniques outlined above. Nine such training events were hosted by the department during this period. • Present research findings to Geographic Combatant Commanders, Ministries of Public Health, international conference proceedings, and peer reviewed publications. For this reporting period, this included presentations to 12 international conferences and 17 peer-reviewed publications on relevant military and public health disease threats. • Conducted 22 diverse research projects on primary disease threats to military and civilian populations of public health relevance • Merged with our fellow AFRIMS Department of Immunology and Medicine to create the Department of Bacterial and Parasitic Diseases, and in doing so efficiently combined the best resources to address the top two disease threats to military and civilian populations: malaria and diarrheal disease Department of Entomology Mission: The mission of the Department of Entomology is to (1) develop and evaluate interventions and vector control products to mitigate vector-borne diseases, (2) provide entomological support for drug and vaccine studies, and (3) conduct comprehensive surveillance for vectors and pathogens of military importance. Discuss Significant Activities Related to Organizational Mission: • The Vector Biology and Control section field tested new repellents and traps to repel and trap vectors of malaria, dengue, and Zika. • The Diagnostics and Emerging Disease section reported Bartonella, Anaplasma, and Ehrlichia species from rodents and ectoparasites from Bhutan. • The Malaria section supported WRAIR’s laboratory screening of three anti-malaria drugs and a clinical trial of รcาoยmงานbปinระaจtiาํ oปn 2s56o1f ant1i-0m6 alarAiNaNl UdArLugREsPiOnRTC2a0m18bodia.
• The Vector Biology and Control section field tested new repellents and traps to repel and trap vectors of malaria, dengue, and Zika. สถาบนั วิจัยวทิ ยาศาสตรก ารแพทยท หาร • The Diagnostics and Emerging Disease secAtRioMEnD rFeORpCoESrtReESdEABRCaHrINtoSTnITeUlTlEaO,FAMEnDaICpALlaSCsIEmNCaE,S and Ehrlichia species from rodents and ectoparasites from Bhutan. • The Malaria section supported WRAIR’s laboratory screening of three anti-malaria drugs and a clinical trial of combinations of anti-malarial drugs in Cambodia. • The Leptospirosis section has isolated and sequenced leptospirosis isolates from Cobra Gold surveillance. • The Scrub Typhus and Chigger section (1) discovered and reported the first evidence of scrub typhus from wild rodents and chiggers from Kenya and (2) supported the Royal Thai Army with a scrub typhus outbreak investigation. Constraints/Problems Encountered: • Logistics – The Entomology Department is experiencing significant delays in ordering supplies. Supplies are received four to six months after orders are placed. This timeline delays research. • GEIS Funding Delay – FY18 GEIS funds arrived in June, near the end of the fiscal year. Late receipt of funding delayed initiation of multiple projects and ability to complete projects on original timelines. • IRB Delays – Delays in IRB approval of study protocols has delayed malaria studies. Solutions Devised: • Logistics – Delays in ordering supplies is an unresolved issue and is still causing significant problems and hinders ability for Entomology Department to complete its mission. • GEIS Funding Delay – The Entomology Department started work on GEIS projects and used other funds to order supplies. • IRB Delays – There is no resolution to IRB delays. New Missions Added: • Sand Fly Surveillance and Colonization – The Entomology Department was initiated surveillance for sand flies and is now maintaining a colony of sand flies in its insectary. This work will support research on prevention of leishmaniasis, a vector-borne disease of military interest. • Cobra Gold – The Entomology Department is providing vector-borne disease surveillance support for the multi-national military exercise Cobra Gold and reporting scrub typhus and leptospirosis surveillance results to GEIS. Old Missions Discontinued: NSTR Outcomes: In FY18, the Entomology Department brought in $2.63 million in research funding supporting our scientists to provide 11 trainings for research partners from Thailand, Cambodia, and Laos, and produce14 publications, 16 agreements, and 23 conference presentations. รายงานประจําป 2561 107 ANNUAL REPORT 2018
สถาบันวจิ ัยวิทยาศาสตรก ารแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES Department of Immunology and Medicine Mission: The mission of the Department of Immunology and Medicine is to conduct medical product research, development, and disease surveillance to minimize the threat from malaria. The department also conducts disease surveillance studies to help identify patterns and incidence rates of other diseases of military importance in South East Asia. The department also plays an important role in evaluating new diagnostic tests under field conditions. Discuss Significant Activities Related to Organizational Mission: 1. Manages the Malaria Drug Discovery and Advanced Development 1.1. The department conducts clinical trials at established field sites in Cambodia and Thailand, with a focus on therapeutic efficacy studies (TES) of antimalarial drugs and new drug combinations for areas of multi-drug resistance. 1.2. The department maintains robust research platforms and clinical site capabilities, staffed by a highly motivated and competent scientists to deliver requirement-driven biomedical products to protect US military from malaria and bacterial infections. 1.3. Conducts preclinical studies of new drug candidates for the US Military Malaria Research Program (MMRP), the Military Infectious Diseases Research Program (MIDRP), and for external collaborators engaged in malaria drug development. These studies are conducted in nonhuman primates in collaboration with Veterinary Medicine Department. Pharmacokinetic-pharmacodynamics (PKPD) analysis of new drug candidates include all of the following: • Drug identity and purity testing • Uniformity of dosage tests • Correlation of drug levels to clinical outcomes • POPPK modeling 2. Conducts Antimicrobial and Malaria Drug Resistance Surveillance 2.1. Monitors drug resistance for commonly used antimalarials 2.2. Studies antimicrobial drug resistance among high risk military populations 2.3. Characterizes antibiotic resistance profiles and virulence factor genes using traditional culture and molecular biology platforms 2.4. Monitors artemisinin resistance and its implications on treatment outcomes 2.5. Conducts in vitro drug sensitivity assays to characterize the drug susceptibility phenotype of clinical P. falciparum isolates 2.6. Evaluates field isolates for new genetic markers of anti-malarial drug resistance 3. Supports Malaria Vaccine Program and Influenza Surveillance Studies 3.1. Tests new regimens of vaccines for their ability to induce protective immune responses against primary infection and relapse using a relapsing P. cynomolgi rhesus model 3.2. Studies immunity that may be required for protection against localized infections such as influenza, TB and malaria and conducts the research on Zika virus (ZIKV) by examining the generation of memory T cells in rhesus monkeys รายงานประจาํ ป 2561 108 ANNUAL REPORT 2018
สถาบันวจิ ยั วิทยาศาสตรก ารแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 3.3. Supports GEIS Respiratory Surveillance project of pathogens that cause Influenza-Like-Illness (ILI) and monitors for emergence of highly pathogenic influenza strains among military and civilian populations in Cambodia 4. Regional Malaria Elimination Initiatives Supports regional malaria elimination initiatives to include studies aimed at identifying the most effective, appropriate, implementable strategies for military forces stationed along the Cambodia-Thai border. 5. Defense Malaria Assistance Program (DMAP) The program was established in 2017 to target military and associated malaria high risk groups. The program objectives include: 5.1. Execute and maintain joint DoD Mil-Mil operational research program to mitigate DoD malaria threat 5.2. Enhance DoD research relevance in anticipation of future research priorities 5.3. Optimize impact of DoD malaria products in military deployment settings 5.4. Enhance FHP by reducing malaria risk to mil-mil operations personnel 6. Public Health Response The department assists the Ministry of Health in infectious disease outbreak investigation and response. Constraints/Problems Encountered: • Procurement Process – The procurement process is slow and complicated causing delays in ordering supplies, reagents, and laboratory equipment. These delays impacted study initiation timelines. • Workload for clinical studies and diagnostic laboratories is high. Hiring process is complicated, administratively burdensome, and number of trained candidates with appropriate background is low. Solutions Devised: • Procurement Process – Streamlined the process and addressed the communication problems which resulted in faster processing of requests • Staffing and Workload – Improvements in management systems and better delegation of roles and responsibilities resulted in more efficient systems; new hiring actions are pending. New Missions Added: • Established new partnerships in Cambodia with village malaria workers, health centers, malaria supervisors, and host-nation hospitals that are located within disease endemic areas. Similarly, expanded DoD collaborations with NAMRU-2, MRD-Africa, and NAMRU-6 on projects of mutual interest, resulting in better assay harmonization between labs • Received IRB approval and initiated enrollment of volunteers for a new clinical trial “Multicenter Therapeutic Efficacy Assessment of Pyronaridine-Artesunate (Pyramax®) and new drug combinations with Atovaquone-Proguanil for the treatment of uncomplicated P. falciparum malaria in Cambodia”. This study aims to identify new drug regimens that are effective against the most resistant strains of malaria in Cambodia. The proposed combinations may delay the spread of drug resistance and prolong the lifespan of currently available drugs, such as atovaquone-proguanil. รายงานประจําป 2561 109 ANNUAL REPORT 2018
สถาบนั วจิ ยั วิทยาศาสตรการแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES • Expanded the monitoring of an in vitro and molecular markers of antimalarial drug resistance for malaria along Thai-Cambodia borders • Established in vitro culture for P. vivax • Established in vitro system to evaluate new drug combinations for synergy to optimize the selection of drugs for clinical trials • Conducted training on radical cure treatment and G6PD screening in support of the WHO and regional efforts to expand the access to curative doses of primaquine, and in the near future tafenoquine • Initiated Royal Thai Army (RTA) Unit Based Surveillance (UBS) at Thai-Myanmar and Thai-Cambodia Borders • Established key stakeholder engagements with Laos militaries to include meeting and discussions on the new work plan under DMAP program Old Missions Discontinued: NSTR Outcomes: • Assisted in data analysis and publication of Phase III study (WR2134) that evaluated tafenoquine in Cambodian volunteers. Study provided essential data for the drug filing application with the FDA for two separate indications, radical cure and chemoprophylaxis. Tafenoquine has the potential to become an important drug to support malaria elimination efforts throughout Southeast Asia. • Study outcomes from therapeutic efficacy study of atovaquone-proguanil (malarone) and artesunate atovaquone proguanil provided new information on the role of malarone for the areas of MDR in Cambodia. • Completed data analysis for WR2211 with demonstrated reduction of malaria in 8 military camps. Although underpowered to evaluate insecticide treated uniforms from the other interventions, the study outcomes from this Pilot Elimination Study in Cambodia provided new information on the likely benefit of insecticide treated uniforms in reducing the number of malaria infections during 6 month follow-up period, when the ITUs are deployed under field conditions, in the high malaria endemic areas near Cambodia-Thai border. Study outcomes were also presented during the WHO meeting in Geneva, Switzerland and helped guide new recommendations for chemoprophylaxis among mobile and military populations. Publication of the results is planned for later this year. • Surveillance of molecular markers provided important insights into the drug resistance of Plasmodium parasites and informed new studies that will be instrumental to malaria control in Southeast Asia. • Assisted Ministry of Public Health in the investigation of malaria outbreak. Led efforts, along with Ministry of Health staff, to characterize a malaria outbreak in Sisaket province with nearly 4 thousand people screened for malaria in the areas of the reported outbreak • The surveillance data on drug resistance helped to characterize the actual threat from malaria with ‘triple mutant’ phenotype, which was first detected in Cambodia in 2017. • Using our field isolates, conducted drug susceptibilities to WRAIR lead compounds (i.e. Triazines), helping in down-selecting the best candidates • Characterized the antimicrobial drug susceptibilities among bacterial pathogens responsible for diarrheal illness in Cambodia รายงานประจําป 2561 110 ANNUAL REPORT 2018
สถาบันวิจยั วิทยาศาสตรก ารแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES • Conducted the first primaquine pharmacokinetic study in healthy Cambodians to assess the effect of CYP2D6 genotype on relapse • Completed an operational research study on G6PD screening tests which provided valuable information to national malaria programs on the test performance, and community perceptions of risk with radical cure treatment. The lessons learned and challenges with implementation of new diagnostic tests will be important in anticipation of wide-spread use of primaquine for radical cure of P. vivax. • Provided latest data for antiviral susceptibility of influenza viruses circulating in Cambodia • Established the ex vivo Ring-Stage Survival Assay (RSA) and harmonized the assay between other DoD labs • Supported External Competency Assessment of Malaria Microscopists (ECAMM) for RCAF and CNM staff in collaboration with WHO WPRO • Continued to build the bench by hosting infectious disease fellows, residents, and active duty personnel for 1 month rotation in overseas laboratories and field sites. Department of Retrovirology Mission: The mission of the Department of Retrovirology is to support the mission of the Military HIV Research Program (MHRP) to protect the US Military from HIV and improve global health by conducting research to develop an HIV vaccine, reduce new infections and find a cure. Discuss Significant Activities Related to Organizational Mission: • Identify and characterize cohorts of volunteers at high risk of HIV for future clinical research studies in Thailand, principally through a study called RV348, at four sites in Thailand • Conduct acute HIV infection studies as a platform for laboratory research to better understand infection, as well as a platform for cure research clinical trials • Conduct HIV cure studies evaluating novel approaches including a therapeutic vaccine and a monoclonal antibody after two different durations of time in volunteers found to be acutely infected with HIV • Evaluate novel approaches to cure HIV-like viruses in non-human primate models to understand HIV-related biology and model potential therapeutic interventions or vaccines • Established new assays to be able to assess functions of granulocytes, and antibody- mediated immune cell functions that we could not previously assess in our vaccine, cure, or non- human primate studies Constraints/Problems Encountered: Significant reductions in funding required closure of RV217 in FY18, which is one year earlier than the original plan that had been planned to finish follow-up of RV217 (i.e. all clinical visits) in FY19. รายงานประจาํ ป 2561 111 ANNUAL REPORT 2018
สถาบนั วิจยั วทิ ยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES Solutions Devised: Responsibly drew RV217 to a close through careful coordination with all stakeholders, especially the participants, regarding study closure. Communicated with the community advisory board and community at large regarding study outcomes and results New Missions Added: None Old Missions Discontinued: None Outcomes: • The Department of Retrovirology’s ability to conduct clinical trials meeting local and international regulatory standards is supported by its exemplary College of American Pathologists (CAP) certified AFRIMS Retrovirology Clinical Laboratory (ARCL), specimen processing and archiving laboratory, and its basic science research program. CAP re-accreditation is valid through September 2020. • Successful on-schedule enrollment of RV348, a high-risk HIV incidence cohort independently assessing four locations for inclusion as future vaccine efficacy trial sites. The tentative completion of clinical activities will be December 2019. o RV348B (Bangkok): A total of 1,017 participants were enrolled (502 participants at Vaccine Trial Centre, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, and 515 at Royal Thai Army Clinical Research Center, AFRIMS). o RV348M (MoPH): A total of 1,003 participants were enrolled (503 participants at Potharam, Ratchaburi and 500 participants at Nakorn Ratchasima). • RV398, conducted in collaboration with the NIH Vaccine Research Center and the US Military HIV Research Program (MHRP) designed to investigate whether a very potent and broad antibody product given to acute HIV infected volunteers either before, with, or after interruption of antiretroviral treatment will benefit the volunteers, including limiting the amount of virus in their bodies and possibly cure, has enrolled the majority of volunteers in this multicenter study. Currently, 6 participants are eligible and recruited in this study. • In support of cure research, the department successfully conducts of a SHIV cure study in non-human primates. This study is investigating whether a combination of two investigational therapies can cure or otherwise improve the health of monkeys infected with a virus very similar to HIV, hopefully paving the way for the ability of HIV-infected service members to remain clinically stable without the daily burden and lifetime cost of antiretroviral therapy. • The department has implemented Cambridge Neuropsychological Test Automated Battery (CANTAB), a neurobehavioral testing platform in rhesus macaques in collaboration with the Department of Veterinary Medicine. This platform will be used to correlate neurocognitive changes with neuropathologies in rhesus macaques. • In support of acute HIV infection and HIV cohort studies, the department has developed two molecular assays in collaboration with MHRP; o Multi-Region Subtype Specific PCR (MSSP) assay to characterize HIV-1 subtypes and their recombinant viruses circulating in Thai HIV-infected participants o Total HIV DNA assay to quantify all forms of HIV DNA in Thai HIV-infected participants • The department has developed new capabilities to use in support ongoing studies; o Granulocyte isolation and functional characterization รายงานประจําป 2561 112 ANNUAL REPORT 2018
สถาบนั วจิ ยั วิทยาศาสตรก ารแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES o Antibody-dependent complement-mediated lysis is being developed for use by MHRP to further characterize non-neutralizing antibodies and their contribution to the immune response in the vaccine and cure studies. • RV397 (VRC01 monoclonal antibody given to HIV infected volunteers): Total of 23 participants were enrolled, 18 participants underwent treatment interruption and received study infusions according to study protocol. Only one participant achieved the primary endpoint of HIV viral load (VL) < 50 copies/mL at 24 weeks after treatment interruption. Median time to VL rebound above 20 copies/mL was 29 days (range 9-296) for the VRC01 arm and 14 days (range 14-29) for the placebo arm (p = 0.051 for Kaplan-Meier test). All participants achieved VL < 20 copies/mL within 5 weeks after ART resumption. Completion of clinical activity and continue laboratory and data analysis phase • RV217 (Early Capture acute HIV Cohort): For Thailand, a total of 1,413 potential volunteers were screened and 947 participants (67%) enrolled the study; 71 incident cases were identified. Clinical activities were completed this year. Laboratory and data analysis are ongoing. • RV405 (HIV therapeutic vaccines for HIV infected volunteer): 27 participants enrolled in this study and in follow up phase. There was no clinical abnormality finding. Completion of vaccinations and clinical activities and now at laboratory and data analysis phase • RV305 (Phase 2 late boost follow-on study to RV144): A total of 164 participants were enrolled and completed all study vaccination, showing benefit to late boost, ongoing laboratory and data analysis. • RV306 (Phase 2 evaluation of various one-year boosts following the RV144 regimen in previously unvaccinated volunteers): 367 volunteers were enrolled, and all study visits were completed. Late boosting preferentially improves plasma IgG over IgA responses and neutralization activity as well as cellular immune functions, ongoing laboratory and data analysis. • RV328 (Evaluating the protein boost used in RV144 and a previous efficacy trial in injecting drug users called Vax003): A total of 40 participants completed all vaccinations and clinical activities. The study is now at laboratory analysis phase. Department of Veterinary Medicine Mission: The mission of the Department of Veterinary Medicine is to protect military personnel and their families against tropical disease threats through pre-clinical product development of new prophylactic and therapeutic drugs and new or improved vaccines. Discuss Significant Activities Related to Organizational Mission: To fulfill this mission, the Department of Veterinary Medicine (DVM): • Conducts bio-medical research in animal models and zoonotic disease surveillance • Provides veterinary training and expertise in the areas of animal research, laboratory animal care, animal pathology, Institutional Animal Care and Use Committee to students, faculty and potential collaborators • Maintains research animals that are free of potential confounding diseases • Ensures that all animals receive humane, proper, and safe care and that the USAMD- AFRIMS' Animal Care and Use Program complies with appropriate laws, regulations and guidelines รายงานประจาํ ป 2561 113 ANNUAL REPORT 2018
สถาบันวจิ ยั วทิ ยาศาสตรการแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES Constraints/Problems Encountered: • Procurement Process – The procurement process is slow and complicated causing delays in ordering supplies (animal feed, bedding, enrichment items such as fresh fruits and vegetables, etc.) and laboratory equipment. These delays have a negative impact on the animal colony maintenance and research study time plan. • Facilities – ABSL-3 containment, building 5 cannot be used due to the ventilation system failure. BioBubble installed in room A6 cannot be utilized since it is waiting for commissioning (project undergoing). Additionally, there is no elevator to clinical pathology laboratory or laundry room on 2nd floor. It is very difficult to move freezers, big equipment and heavy items. Oftentimes this has resulted in damaged equipment and facilities in addition to risks to personnel carrying the equipment. • Storage Space – Very limited space in clinical pathology laboratory and animal vivarium. Inadequate space to store and maintain tissue blocks, documents, supplies, equipment, animal cages and accessories • Staff Shortages – Heavy workload in each section due to vacated positions and not enough manpower to sustain the mission. Additionally, the prolonged hiring process, in processing/ clearance of new personnel has, in some circumstances, negatively impacted our ability to complete our mission. • Animal Facility Renovation Projects and Nonhuman Primates Cage Modification – Projects have been prolonged and contractors cannot finish the projects within time. This affects the animal colony management and research activities. • Pest Control Management – The current pest control program does not effectively control all pests that have been found within the animal facility. Additionally, the lack of airlocks at animal facility access points (i.e. loading dock corridors 9 and B12) complicate the problem. • Grounds Maintenance – Currently in need of a gardener who can be responsible for cleaning litter, leaves, and cutting or trimming the trees around the facility Solutions Devised: • Procurement Process – Delays in ordering items/supplies is an unresolved issue and is still causing significant problems and hinders ability for Veterinary Medicine Department to carry out its mission. • Facility – Ongoing project to address ABSL-3 and BioBubble issue. No solution for the lack of elevator • Facility Space – No resolution yet • Staff Shortage – Unresolved issue • Facility Renovation Projects – No solution yet. This will require professional and reliable contractors to perform the renovation or modification. • Pest Control – Working with facilities on this issue • Gardener – Unresolved concern New Missions Added: • Immunology – Clinical pathology laboratory recently established new laboratory capabilities for immunology (flow cytometry and ELISpot assays) and molecular biology (qPCR and cell culture). รายงานประจาํ ป 2561 114 ANNUAL REPORT 2018
สถาบันวิจยั วทิ ยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES • Genetic Typing – Clinical pathology laboratory recently established a DNA paternity testing for monkey colony. • Currently determining the feasibility and work towards bringing to AFRIMS the ability to harvest, process, and store our own NHP hepatocytes in support of our Malaria protocols • Exploring collaborator’s interest and the ability of the DVM to bring new animal species and animal models to AFRIMS • Continued to use and improve animal models of malaria in nonhuman primates. This effort will include not only the relapsing malaria model for Plasmodium cynomolgi but also a model of severe cerebral malaria (Plasmodium coatneyi) and the fifth human malaria Plasmodium knowlesi. • Working on the development of new animal models of human disease, including a natural challenge Rhesus scrub typhus model in collaboration with Mahidol-Oxford Research Unit, Naval Medical Research Center, and Louisiana State University • Rhesus dengue vaccine models for two large long-term immunity studies • Continued to develop partnerships for testing vaccines and therapeutics against dengue fever and ZIKA virus in the rhesus macaque model • Continued to develop animal models of human disease, including a Rhesus simian- human immunosuppressive virus (SHIV) model in collaboration with the Military HIV Research Program (MHRP) • Developed the training program in rhesus monkeys for blood collection techniques utilizing positive reinforcement and the training program in rhesus monkeys to utilize CANTAB apparatus for evaluating the cognitive/memory and motor (behavioral) changes in SHIV infected rhesus monkeys • Provided expertise and assistance supporting the development of a regional primate center and other research organizations in Thailand • Provided expertise, training and assistance to the Chulalongkorn University, Department of Veterinary Pathology, through technician exchanges and direct training of faculty and students • Continued to support the USAMD-AFRIMS research mission by providing veterinary expertise and animal resources for product testing Old Missions Discontinued: NSTR Outcomes: • Antimalarial Drug Efficacy Testing in the Rhesus Monkey (Macaca mulatta)/ Plasmodium cynomolgi Malaria Models: Two studies for prophylaxis and radical curative treatment were conducted in 2018 for WRAIR-ET. Eight (8)-aminoquinoline and Avermectin compounds were tested: 46 monkeys were utilized for testing antimalarial compounds. • Care and Maintenance of Rhesus (Macaca mulatta) Monkeys and Management of Breeding Colonies: Fifty (50) baby rhesus monkeys were born this year. • Care and Maintenance of Laboratory Rodents and Rabbits, Maintenance of Rodent Breeding Colonies and Quality Assurance/ Quality Surveillance Program: 5,917 ICR mice (Mus musculus) were produced, and 2,686 ICR mice were used for seven active protocols. • Mosquito and Sand Fly Feeding Using in Vitro and in Vivo Techniques with Mice (Mus musculus) as a Blood Source: 173 culled mice were used to maintain mosquito and sand fly colonies. รายงานประจาํ ป 2561 115 ANNUAL REPORT 2018
สถาบนั วิจัยวทิ ยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES • Maintenance of the Leptotrombidium Larval Mite Colonies: Chigger Feeding on ICR Mice (Mus musculus). 600 mice were used to maintain the chigger mite colony. • Inoculation of Dengue Viruses in Suckling Mice: 1,269 mice were used to produce high titers of dengue virus antigens. • Production of Plasmodium cynomolgi-Infected Blood Products Utilizing a Rhesus Monkey (Macaca mulatta) Malaria Model: Three (3) monkeys were employed for producing infective P. cynomolgi blood products to be provided to research collaborators on a pre-determined schedule for in vitro or in vivo research efforts, which may include evaluation and screening of novel antimalarial compounds or vaccine discovery efforts. • Bio-Sample Collection Procedures for Mice and Nonhuman Primates: 11 mice and 22 monkeys were utilized this year to provide blood, blood products, or other biological samples (including urine, cervical vaginal mucosal samples, semen and anal mucosal samples) from normal, healthy animals to investigators, veterinarians, and diagnostic laboratories supported by AFRIMS and other DoD institutes. • In Vivo Virulence Determination of Different O. tsutsugamushi Strains in Mouse Models: 82 mice were used to determine the virulence of O. tsutsugamushi strain Karp and UT176 and Comparing in vivo virulence of high passage and low passage of O. tsutsugamushi strain TM4942. • Induction of Protective Immunity Against Primary Infection and Relapse in a Relapsing P. cynomolgi Rhesus Model by Chemoprophylaxis with Sporozoite (CPS) Immunization: Four (4) monkeys were used this year to assess the standard inoculation dose for inducing relapse using the P. cynomolgi rhesus monkeys. • Efficacy of Latency Reversing Agent TLR7 Agonist GS-986 and HIV-Specific Broadly Neutralizing Antibodies N6-LS and PGT121 to Achieve Virologic Remission in SHIV-Infected Rhesus Macaques (Macaca mulatta) that Initiated Antiretroviral Therapy During Acute Infection: 16 rhesus monkeys were all successfully inoculated with SHIV and subsequently all initiated on antiretroviral therapy for the treatment of acute SHIV infection. • Re-Evaluation of the Rhesus Monkey (Macaca mulatta) Intra-Gastric Challenge Model of Shigellosis with Wild-Type Shigella flexneri 2a 2457T Strain, and Pre-Clinical Evaluation of Hyper-Immune Bovine Colostrum Against Wild Type Shigella flexneri Strain in the Rhesus Monkey Challenge Model: 28 naïve rhesus monkeys were utilized for Shigella model validation and testing hyper-immune bovine colostrum (HBC). • Characterization of Host Pathology and Immunity in Response to Orientia tsutsugamushi Infection Following Chigger Bite: 414 ICR mice were used for determination of the effect of chigger feeding duration on transmission efficiency of O. tsutsugamushi to host. • Induction of Protective Immunity Against Primary Infection and Relapse in a Relapsing P. cynomolgi Rhesus Model Using Two Regimens of Chemoprophylaxis with Sporozoite (CPS) Vaccine: 18 rhesus monkeys were used for evaluating two regimens of chemoprophylaxis with sporozoites immunization for their ability to induce protective immunity. Five (5) splenectomized rhesus monkeys were used as malaria donor monkeys. • Evaluation of Scrub Typhus Vaccine Based on TSA56 and ScaA Proteins in Rhesus Macaque Model: A total of nine (9) rhesus monkeys were utilized for testing a novel vaccine antigen for scrub typhus. • Testing of the Novel Compound (Anti-Von Willebrand Factor Aptamer) for the Treatment of Severe Malaria Using Rhesus Macaques (Macaca mulatta)/Plasmodium coatneyi Model: Two (2) rhesus monkeys were utilized for testing the infectivity and virulence of frozen Plasmodium coatneyi stock. รายงานประจําป 2561 116 ANNUAL REPORT 2018
สถาบันวจิ ัยวิทยาศาสตรการแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES • Teaching and Training Protocol Using Small Animals and Rhesus Monkeys: 137 ICR mice were used for hand on training and 19 rhesus monkeys were trained to be familiar with the CANTAB apparatus in support of HIV/SHIV research. Department of Virology Mission: The mission of the department is to develop and evaluate products, and collect epidemiologic data to defeat infection. Discuss Significant Activities Related to Organizational Mission: Our activities revolve around several pillars: • Conduct advanced phase clinical trials to evaluate vaccine and drug products • Evaluate and validate diagnostic devices and kits • Conduct expansive regional surveillance in order to both determine where to conduct clinical trials and to determine evolving pathogen threats of importance to the COCOMs • Assay development in the laboratory to better identify pathogens identified in or studied in surveillance studies, clinical trials and diagnostic device evaluation Constraints/Problems Encountered: • Cebu Facilities – There was a previous plan to have the department’s Cebu laboratory occupy space on the grounds of a large local public hospital. This proved unworkable and over the last several years the department has been renting and developing commercial space. However, this lease is managed by the US Army Corps of Engineers who have repeatedly failed to renew the lease in a timely fashion. This runs an existential risk to the functioning of the Cebu, Philippines laboratory. • Maintain Sufficient Military Personnel – In 2009, with a department of half the size of its current composition, the department was previously manned with 3 senior physicians and 1 experienced microbiologist. Since 2014, the department has been manned with only 2 physicians. • Timely Receipt of Awarded Funds – A large portion of the department’s annual operative budget comes from GEIS (The Global Emerging Infectious Disease Surveillance) program. Work on such projects is expected to begin 1 October or each year, but funds are often not received until late February of the following year. • Increasing Difficult in Sending Specimens Outside Our Partner Countries – There has been an increasing and spreading move towards our partner countries more highly regulating the sending of specimens to laboratories outside of the country of origin of the specimens. The move is driven to ensure greater control over indigenous specimens and to encourage capacity building within countries. Solutions Devised: • Cebu Facilities – The department has appointed a point person in the department to continuously follow-up, write and call USACE. This has only been marginally impactful. Working with USACE has proven to be extremely difficult regardless of the personnel assigned over the last 5 years. รายงานประจาํ ป 2561 117 ANNUAL REPORT 2018
สถาบนั วิจัยวทิ ยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES • Maintain Sufficient Military Personnel – The department chief and AFRIMS Director have worked aggressively to locate appropriate staff to at least maintain current staffing and the WRAIR Commander and AFRIMS Director have acquired a status of tier one for our staffing needs. • Timely Receipt of Awarded Funds – The department works to utilize existing multi- year funds in order to meet short-term needs pending receipt of the targeted funds, but the solution is suboptimal. • Increasing Difficult in Sending Specimens Outside Our Partner Countries – The department is actively seeking to push higher level of technology downwards to our local laboratories and to our partners in order to allow more to be done locally, decreasing the impact of this issue on the mission. New Missions Added: Engagement in India – We have initiated this year a new relationship with a partner in India, THSTI (Translational Health Science and Technology Institute) of the Indian Ministry of Health as a gateway to explore future and expanding collaborative projects consistent with INDOPACOM goals. Old Missions Discontinued: None Outcomes: • We have neared the end of a large phase III dengue vaccine trial with a product manufactured by Sanofi. This vaccine has proven effective in reducing severe diseases and hospitalization in patients with pre-existing exposure to dengue and also offers moderate protection against infection. However, it may increase severity of disease in those not previously infected who become infected a year after completing the vaccination series and is poorly protective against infection as well in such populations. • We have achieved the primary endpoint in another phase III dengue vaccine study with a product manufactured by Takeda pharmaceuticals Inc. This product showed broad protection against dengue infection during the first year following vaccination. It remains to be seen what the protection or risk assessment is in the following years. • We completed a phase II study with the National Institute of Health dengue vaccine. Detailed results are still pending, but it appears that this vaccine may have the most robust immunological response of the three vaccines assessed by the department. • We have continued to identify shifting trends in both arboviral and upper respiratory viruses circulating in the region and provided this information to the COCOMs and to DoD authorities that communicate with the CDC. รายงานประจําป 2561 118 ANNUAL REPORT 2018
สถาบันวจิ ยั วิทยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES AFRIMS Publications and Presentations FY18 (1 October 2017 - 30 September 2018) Publications 1. Akapirat S; Karnasuta C; Vasan S; Rerks-Ngarm S; Pitisuttithum P; Madnote S; Savadsuk H; Rittiroongrad S; Puangkaew J; Phogat S; Tartaglia J; Sinangil F; de Souza MS; Excler JL; Kim JH; Robb ML; Michael NL; Ngauy V; O'Connell RJ; Karasavvas N; on behalf of The RV305 Study Group. Characterization of HIV-1 gp120 antibody specificities induced in anogenital secretions of RV144 vaccine recipients after late boost immunizations. PLoS One 2018 Apr; 13(4):e0196397. 2. Ake JA; Schuetz A; Pegu P; Wieczorek L; Eller MA; Kibuuka H; Sawe F; Maboko L; Polonis V; Karasavva N; Weiner D; Sekiziyivu A; Kosgei J; Missanga M; Kroidl A; Mann P; Ratto-Kim S; Eller LA; Earl P; Moss B; Dorsey-Spitz J; Milazzo M; Ouedraogo GL; Rizvi F; Yan J; Khan AS; Peel S; Sardesai NY; Michael NL; Ngauy V; Marovich M; Robb ML. Safety and immunogenicity of PENNVAX(R)-G DNA prime administered by Biojector(R) 2000 or CELLECTRA(R) electroporation device with modified vaccinia Ankara-CMDR Boost. J Infect Dis 2017 Nov; 216(9):1080-90. 3. Anderson KB; Simasathien S; Watanaveeradej V; Weg AL; Ellison DW; Suwanpakdee D; Klungthong C; Phonpakobsin T; Kerdpanich P; Phiboonbanakit D; Gibbons RV; Fernandez S; Macareo LR; Yoon IK; Jarman RG. Clinical and laboratory predictors of influenza infection among individuals with influenza-like illness presenting to an urban Thai hospital over a five-year period. PLoS One 2018 Mar; 13(3):e0193050. 4. Chao CC; Ingram BO; Lurchachaiwong W; Ching WM. Metabolic characterization of serum from mice challenged with Orientia tsutsugamushi-infected mites. New Microbes New Infect 2018 May; 23:70-6. 5. Chenine AL; Merbah M; Wieczorek L; Molnar S; Mann B; Lee J; O'Sullivan AM; Bose M; Sanders-Buell E; Kijak GH; Herrera C; McLinden R; O'Connell RJ; Michael NL; Robb ML; Kim JH; Polonis VR; Tovanabutra S. Neutralization sensitivity of a novel HIV-1 CRF01_AE panel of infectious molecular clones. J Acquir Immune Defic Syndr 2018 Jul; 78(3):348-55. 6. Colby DJ; Trautmann L; Pinyakorn S; Leyre L; Pagliuzza A; Kroon E; Rolland M; Takata H; Buranapraditkun S; Intasan J; Chomchey N; Muir R; Haddad EK; Tovanabutra S; Ubolyam S; Bolton DL; Fullmer BA; Gorelick RJ; Fox L; Crowell TA; Trichavaroj R; O'Connell R; Chomont N; Kim JH; Michael NL; Robb ML; Phanuphak N; Ananworanich J; RV411 study group. Rapid HIV RNA rebound after antiretroviral treatment interruption in persons durably suppressed in Fiebig I acute HIV infection. Nat Med 2018 Jul; 24(7):923-6. 7. Colston JM; Ahmed AMS; Soofi SB; Svensen E; Haque R; Shrestha J; Nshama R; Bhutta Z; Lima IFN; Samie A; Bodhidatta L; Lima AAM; Bessong P; Paredes Olortegui M; Turab A; Mohan VR; Moulton LH; Naumova EN; Kang G; Kosek MN; Mal-Ed network. Seasonality and within-subject clustering of rotavirus infections in an eight-site birth cohort study. Epidemiol Infect 2018 Apr; 146(6):688-97. 8. Congpuong K; Ubalee R. Population genetics of Plasmodium vivax in four high malaria endemic areas in Thailand. Korean J Parasitol 2017 Oct; 55(5):465-72. 9. Crawford MA; Margulieux KR; Singh A; Nakamoto RK; Hughes MA. Mechanistic insights and therapeutic opportunities of antimicrobial chemokines. Semin Cell Dev Biol 2018 Feb; [Epub ahead of print]. 119 ANNUAL REPORT 2018 รายงานประจาํ ป 2561
MN; Mal-Ed network. Seasonality and within-subject clustering of rotavirus infections สiถnาบaนั nวิจeัยiวgทิ hยtา-ศsาiสtตeรกbาiรrแtพhทcยoทหhาoรrt study. Epidemiol Infect 2018 Apr; 146(6):688-97. 8. ARCMoEDnFgOpRCuESoRnESgEAKRCH; IUNSTbITaUlTeEeOFRM.EDPICoALpSuClIEaNtCiEoSn genetics of Plasmodium vivax in four high malaria endemic areas in Thailand. Korean J Parasitol 2017 Oct; 55(5):465-72. 9. Crawford MA; Margulieux KR; Singh A; Nakamoto RK; Hughes MA. Mechanistic insights and therapeutic opportunities of antimicrobial chemokines. Semin Cell Dev Biol 2018 Feb; [Epub ahead of print]. 10. Crowell TA; Colby DJ; Pinyakorn S; Fletcher JLK; Kroon E; Schuetz A; Krebs SJ; Slike BM; Leyre L; Chomont N; Jagodzinski LL; Sereti I; Utay NS; Dewar R; Rerknimitr R; Chomchey N; Trichavaroj R; Valcour VG; Spudich S; Michael NL; Robb ML; Phanuphak N; Ananworanich J; for the RV254/SEARCH010 Study Group. Acute retroviral syndrome is associated with high viral burden, CD4 depletion, and immune activation in systemic and tissue compartments. Clin Infect Dis 2018 May; 66(10):1540-9. 11. Dawood FS; Hunt D; Patel A; Kittikraisak W; Tinoco Y; Kurhe K; Soto G; Hombroek D; Garg S; Chotpitayasunondh T; Gonzales O; Bhargav S; Thompson MG; Chotpitayasunondh B; Florian R; Prakash A; Arriola S; Macareo L; Das P; Cabrera S; La Rosa S; Azziz-Baumgartner E; Pregnancy and Influenza Multinational Epidemiologic (PRIME) Study Working Group. The Pregnancy and Influenza Multinational Epidemiologic (PRIME) study: a prospective cohort study of the impact of influenza during pregnancy among women in middle-income countries. Reprod Health 2018 Sep; 15(1):159. 12. Dumre SP; Bhandari R; Shakya G; Shrestha SK; Cherif MS; Ghimire P; Klungthong C; Yoon IK; Hirayama K; Na-Bangchang K; Fernandez S. Dengue virus serotypes 1 and 2 responsible for major dengue outbreaks in Nepal: clinical, laboratory, and epidemiological features. Am J Trop Med Hyg 2017 Oct; 97(4):1062-9. 13. Ewers EC; Anisowicz SK; Ferguson TM; Seronello SE; Barnhill JC; Lustik MB; Agee W 3rd; Washington MA; Nahid MA; Burnett MW; Bodhidatta L; Srijan A; Rukasiri S; Wassanarungroj P; Ruekit S; Nobthai P; Swierczewski BE; Lurchachaiwong W; Serichantalergs O; Ngauy, Viseth Ngauy V. Antibiotic resistance, molecular characterizations, and clinical manifestations of campylobacteriosis at a military medical center in Hawaii from 2012-2016: a retrospective analysis. Sci Rep 2018 Aug; 8(1):11736. 14. Fukuda MM; Krudsood S; Mohamed K; Green JA; Warrasak S; Noedl H; Euswas A; Ittiverakul M; Buathong N; Sriwichai S; Miller RS; Ohrt C. A randomized, double-blind, active-control trial to evaluate the efficacy and safety of a three day course of tafenoquine monotherapy for the treatment of Plasmodium vivax malaria. PLoS One 2017 Nov; 12(11):e0187376. 15. Gonwong S; Chuenchitra T; Khantapura P; Islam D; Ruamsap N; Swierczewski BE; Mason CJ. Nationwide seroprevalence of leptospirosis among young Thai men, 2007-2008. Am J Trop Med Hyg 2017 Dec; 97(6):1682-5. 16. Hang J; Zavaljevski N; Yang Y; Desai V; Ruck RC; Macareo LR; Jarman RG; Reifman J; Kuschner RA; Keiser PB. Composition and variation of respiratory microbiota in healthy military personnel. PLoS One 2017 Dec; 12(12):e0188461. 17. Heger E; Schuetz A; Vasan S. HIV vaccine efficacy trials: RV144 and beyond. Adv Exp Med Biol 2018 Jul; 1075:3-30. 18. Hsu DC; Ananworanich J. Immune interventions to eliminate the HIV reservoir. Curr Top Microbiol Immunol 2017; 417:181-210. รายงานประจาํ ป 2561 120 ANNUAL REPORT 2018
สถาบันวจิ ัยวทิ ยาศาสตรก ารแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 19. Hsu DC; Sunyakumthorn P; Wegner M; Schuetz A; Silsorn D; Estes JD; Deleage C; Tomusange K; Lakhashe SK; Ruprecht RM; Lombardini E; Im-Erbsin R; Kuncharin Y; Phuang-Ngern Y; Inthawong D; Chuenarom W; Burke R; Robb ML; Ndhlovu LC; Ananworanich J; Valcour V; O'Connell RJ; Spudich S; Michael NL; Vasan S. Central nervous system inflammation and infection during early, non-accelerated simian-human immunodeficiency virus infection in rhesus macaques. J Virol 2018 Jun; 92(11):e00222-18. 20. Kittikraisak W; Suntarattiwong P; Kanjanapattanakul W; Ditsungnoen D; Klungthong C; Lindblade KA; Fernandez S; Dawood FS; Chotpitayasunondh T; Olsen SJ. Comparison of incidence and cost of influenza between healthy and high-risk children <60 months old in Thailand, 2011-2015. PLoS One 2018 May; 13(5):e0197207. 21. Kobylinski KC; Escobedo-Vargas KS; Lopez-Sifuentes VM; Durand S; Smith ES; Baldeviano GC; Gerbasi RV; Ballard SB; Stoops CA; Vasquez GM. Ivermectin susceptibility, sporontocidal effect, and inhibition of time to re-feed in the Amazonian malaria vector Anopheles darlingi. Malar J 2017 Nov; 16(1):474. 22. Lertsethtakarn-Ketwalha P; Silapong S; Sakpaisal P; Serichantalergs O; Ruamsap N; Lurchachaiwong W; Anuras S; Platts-Mills JA; Liu J; Houpt ER; Bodhidatta L; Swierczewski BE; Mason CJ. Travelers' diarrhea in Thailand: a quantitative analysis using TaqMan(R) Array Card. Clin Infect Dis 2018 Jun; 67(1):120-7. 23. Lima AAM; Soares AM; Filho JQS; Havt A; Lima IFN; Lima NL; Abreu CB; Junior FS; Mota RMS; Pan WK; Troeger C; Medeiros PHQS; Vera HN; Prata MMG; McCormick B; McGrath McGrath M; Rogawski E; Houpt E; Platts-Mills J; Gratz J; Samie A; Bessong P; Babji S; Kang G; Shahida Q; Shakoor S; Bhutta Z; Haque R; Ahmed T; Mduma E; Svensen E; Kosek M; Penataro Yori P; Bodhidatta L; Jasmin S; Mason C; Lang D; Gottlieb M; Guerrant RL. Enteroaggregative Escherichia coli subclinical infection and coinfections and impaired child growth in the MAL-ED cohort study. J Pediatr Gastroenterol Nutr 2018 Feb; 66(2):325-33. 24. Linsuwanon P; Krairojananan P; Rodkvamtook W; Leepitakrat S; Davidson S; Wanja E. Surveillance for scrub typhus, rickettsial diseases, and leptospirosis in US and multinational military training exercise Cobra Gold sites in Thailand. US Army Med Dep J 2018 Jan(1-18):29-39. 25. Margulieux KR; Srijan A; Ruekit S; Nobthai P; Poramathikul K; Pandey P; Serichantalergs O; Shrestha SK; Bodhidatta L; Swierczewski BE. Extended-spectrum beta-lactamase prevalence and virulence factor characterization of enterotoxigenic Escherichia coli responsible for acute diarrhea in Nepal from 2001 to 2016. Antimicrob Resist Infect Control 2018 Jul; 7:87. 26. Mattie DR; Wegner MD; Wong BA; James RA; Mumy KL; McInturf SM; Marcel BJ; Sterner TR. Acute and two-week inhalation toxicity studies in rats for polyalphaolefin (PAO) fluid. Ohio: Air Force Research Laboratory, 2017:252 p. 27. Miller LJ; Nasar F; Schellhase CW; Norris SL; Kimmel AE; Valdez SM; Wollen- Roberts SE; Shamblin JD; Sprague TR; Lugo-Roman LA; Jarman RG; Yoon IK; Alera MT; Bavari S; Pitt MLM; Haddow AD. Zika virus infection in Syrian golden hamsters and strain 13 guinea pigs. Am J Trop Med Hyg 2018 Mar; 98(3):864-7. 28. Moranga C; Ayieko C; Awinda G; Achilla R; Moseti C; Ogutu B; Waitumbi J; Wanja E. Stabilization of RDT target antigens present in dried Plasmodium falciparum- infected samples for validating malaria rapid diagnostic tests at the point of care. Malar J 2018 Jan; 17(1):10. รายงานประจาํ ป 2561 121 ANNUAL REPORT 2018
สถาบนั วิจัยวิทยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 29. Morseth MS; Henjum S; Schwinger C; Strand TA; Shrestha SK; Shrestha B; Chandyo RK; Ulak M; Torheim LE. Environmental enteropathy, micronutrient adequacy and length velocity in Nepalese children - the MAL-ED birth cohort study. J Pediatr Gastroenterol Nutr 2018 Aug; 67(2):242-9. 30. Morseth MS; Torheim LE; Chandyo RK; Ulak M; Shrestha SK; Shrestha B; Pripp AH; Henjum S. Severely inadequate micronutrient intake among children 9-24 months in Nepal-the MAL-ED birth cohort study. Matern Child Nutr 2018 Apr; 14(2):e12552. 31. Morseth MS; Torheim LE; Gebremariam MK; Chandyo RK; Ulak M; Shrestha SK; Shrestha B; Henjum S. Tracking of infant and young child feeding practices among 9- to 24-month-old children in Nepal: the MAL-ED birth cohort study. Public Health Nutr 2018 Feb; 21(2):355-64. 32. Mukhopadhyay M; Galperin M; Patgaonkar M; Vasan S; Ho DD; Nouel A; Claireaux M; Benati D; Lambotte O; Huang Y; Chakrabarti LA. DNA vaccination by electroporation amplifies broadly cross-restricted public TCR clonotypes shared with HIV controllers. J Immunol 2017 Nov; 199(10):3437-52. 33. Nisalak A. Laboratory diagnosis of dengue virus infections (Chapter 13). In: Dengue / edited by Nattachai Srisawat, Teerapong Tantawichien; Usa Thisayakorn. Bangkok: Text and General Publication Co.Ltd., 2018:161-75. 34. Olortegui MP; Rouhani S; Yori PP; Salas MS; Trigoso DR; Mondal D; Bodhidatta L; Platts-Mills J; Samie A; Kabir F; Lima A; Babji S; Shrestha SK; Mason CJ; Kalam A; Bessong P; Ahmed T; Mduma E; Bhutta ZA; Lima I; Ramdass R; Moulton LH; Lang D; George A; Zaidi AKM; Kang G; Houpt ER; Kosek MN; on behalf of the MAL-ED Network. Astrovirus infection and diarrhea in 8 countries. Pediatrics 2018 Jan; 141(1):e20171326. 35. Phuphisut O; Ajawatanawong P; Limpanont Y; Reamtong O; Nuamtanong S; Ampawong S; Chaimon S; Dekumyoy P; Watthanakulpanich D; Swierczewski BE; Adisakwattana P. Transcriptomic analysis of male and female Schistosoma mekongi adult worms. Parasit Vectors 2018 Sep; 11(1):504. 36. Pinilla YT; CP Lopes S; S Sampaio V; Andrade FS; C Melo G; Orfano AS; Secundino NFC; Guerra MGVB; Lacerda MVG; Kobylinski KC; Escobedo-Vargas KS; Lopez-Sifuentes VM; Stoops CA; Baldeviano GC; Tarning J; Vasquez GM; Pimenta PFP; Monteiro WM. Promising approach to reducing malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi. PLoS Negl Trop Dis 2018 Feb; 12(2):e0006221. 37. Ratto-Kim S; Schuetz A; Sithinamsuwan P; Barber J; Hutchings N; Lerdlum S; Fletcher JLK; Phuang-Ngern Y; Chuenarom W; Tipsuk S; Pothisri M; Jadwattanakul T; Jirajariyavej S; Sajjaweerawan C; Akapirat S; Chalermchai T; Suttichom D; Kaewboon B; Prueksakaew P; Karnsomlap P; Clifford D; Paul RH; de Souza MS; Kim JH; Ananworanich J; Valcour V. Characterization of cellular immune responses in Thai individuals with and without HIV-associated neurocognitive disorders. AIDS Res Hum Retroviruses 2018 Aug; 34(8):685-9. 38. Ratto-Kim S; Yoon IK; Paris RM; Excler JL; Kim JH; O'Connell RJ. The US military commitment to vaccine development: a century of successes and challenges. Front Immunol 2018 Jun; 9:1397. รายงานประจําป 2561 122 ANNUAL REPORT 2018
สถาบนั วิจยั วิทยาศาสตรการแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 39. Riddle MS; Connor P; Fraser J; Porter CK; Swierczewski B; Hutley EJ; Danboise B; Simons MP; Hulseberg C; Lalani T; Gutierrez RL; Tribble DR; TrEAT TD Study Team. Trial evaluating ambulatory therapy of travelers' diarrhea (TrEAT TD) study: a randomized controlled trial comparing 3 single-dose antibiotic regimens with loperamide. Clin Infect Dis 2017 Nov; 65(12):2008-17. 40. Rodkvamtook W; Kuttasingkee N; Linsuwanon P; Sudsawat Y; Richards AL; Somsri M; Sangjun N; Chao CC; Davidson S; Wanja E; Gaywee J. Scrub typhus outbreak in Chonburi province, central Thailand, 2013. Emerg Infect Dis 2018 Feb; 24(2):361-5. 41. Roth A; Adapa SR; Zhang M; Liao X; Saxena V; Goffe R; Li S; Ubalee R; Saggu GS; Pala ZR; Garg S; Davidson S; Jiang RHY; Adams JH. Unraveling the Plasmodium vivax sporozoite transcriptional journey from mosquito vector to human host. Sci Rep 2018 Aug; 8(1):12183. 42. Roth A; Maher SP; Conway AJ; Ubalee R; Chaumeau V; Andolina C; Kaba SA; Vantaux A; Bakowski MA; Thomson-Luque R; Adapa SR; Singh N; Barnes SJ; Cooper CA; Rouillier M; McNamara CW; Mikolajczak SA; Sather N; Witkowski B; Campo B; Kappe SHI; Lanar DE; Nosten F; Davidson S; Jiang RHY; Kyle DE; Adams JH. A comprehensive model for assessment of liver stage therapies targeting Plasmodium vivax and Plasmodium falciparum. Nat Commun 2018 May; 9(1):1837. 43. Ruksasiri S; Lurchachaiwong W; Wassanarungroj P; Serichantalergs O; Sivhour C; Samon N; Ly S; Chanthap L; Bodhidatta L; Crawford J. Antimicrobial resistant Helicobacter fennelliae isolated from non-diarrheal child stool sample in Battambang, Cambodia. Gut Pathog 2018 May; 10:18. 44. Sakamoto H; Takeo S; Takashima E; Miura K; Kanoi BN; Kaneko T; Han ET; Tachibana M; Matsuoka K; Sattabongkot J; Udomsangpetch R; Ishino T; Tsuboi T. Identification of target proteins of clinical immunity to Plasmodium falciparum in a region of low malaria transmission. Parasitol Int 2018 Apr; 67(2):203-8. 45. Salje H; Cummings DAT; Rodriguez-Barraquer I; Katzelnick LC; Lessler J; Klungthong C; Thaisomboonsuk B; Nisalak A; Weg A; Ellison D; Macareo L; Yoon IK; Jarman R; Thomas S; Rothman AL; Endy T; Cauchemez S. Reconstruction of antibody dynamics and infection histories to evaluate dengue risk. Nature 2018 May; 557(7707):719-23. 46. Sampaio VS; Rivas GBDS; Kobylinski K; Pinilla YT; Pimenta PFP; Lima JBP; Bruno RV; Lacerda MVG; Monteiro WM. What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis. Parasit Vectors 2017 Dec; 10(1):623. 47. Sivro A; Schuetz A; Sheward D; Joag V; Yegorov S; Liebenberg LJ; Yende-Zuma N; Stalker A; Mwatelah RS; Selhorst P; Garrett N; Samsunder N; Balgobin A; Nawaz F; Cicala C; Arthos J; Fauci AS; Anzala AO; Kimani J; Bagaya BS; Kiwanuka N; Williamson C; Kaul R; Passmore JS. Integrin alpha4beta7 expression on peripheral blood CD4(+) T cells predicts HIV acquisition and disease progression outcomes. Sci Transl Med 2018 Jan; 10(425):eaam6354. 48. Smith DR; Sprague TR; Hollidge BS; Valdez SM; Padilla SL; Bellanca SA; Golden JW; Coyne SR; Kulesh DA; Miller LJ; Haddow AD; Koehler JW; Gromowski GD; Jarman RG; Alera MTP; Yoon IK; Buathong R; Lowen RG; Kane CD; Minogue TD; Bavari S; Tesh RB; Weaver SC; Linthicum KJ; Pitt ML; Nasar F. African and Asian zika virus isolates display phenotypic differences both in vitro and in vivo. Am J Trop Med Hyg 2018 Feb; 98(2):432-44. รายงานประจําป 2561 123 ANNUAL REPORT 2018
สถาบันวจิ ัยวทิ ยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 49. Soonwera M; Phasomkulsolsil S. Adulticidal, larvicidal, pupicidal and oviposition deterrent activities of essential oil from Zanthoxylum limonella Alston (Rutaceae) against Aedes aegypti (L.) and Culex quinquefasciatus (Say). Asian Pac J Trop Biomed 2017 Nov; 7(11):967-78. 50. Srijan A; Lurchachaiwong W; Wongstitwilairoong B; Bodhidatta L; Mason C; Swierczewski B. Prevalence and antimicrobial resistance of non-typhoid Salmonella in military personnel, 1988-2013. Asian Pac J Trop Med 2018 May; 11(6):387-92. 51. Srijan A; Margulieux KR; Ruekit S; Snesrud E; Maybank R; Serichantalergs O; Kormanee R; Sukhchat P; Sriyabhaya J; Hinkle M; Crawford JM; McGann P; Swierczewski BE. Genomic characterization of nonclonal mcr-1-positive multidrug- resistant Klebsiella pneumoniae from clinical samples in Thailand. Microb Drug Resist 2018 May; 24(4):403-10. 52. Sunyakumthorn P; Somponpun SJ; Im-Erbsin R; Anantatat T; Jenjaroen K; Dunachie SJ; Lombardini ED; Burke RL; Blacksell SD; Jones JW; Mason CJ; Richards AL; Day NPJ; Paris DH. Characterization of the rhesus macaque (Macaca mulatta) scrub typhus model: Susceptibility to intradermal challenge with the human pathogen Orientia tsutsugamushi Karp. PLoS Negl Trop Dis 2018 Mar; 12(3):e0006305. 53. Takhampunya R; Korkusol A; Promsathaporn S; Tippayachai B; Leepitakrat S; Richards AL; Davidson SA. Heterogeneity of Orientia tsutsugamushi genotypes in field-collected trombiculid mites from wild-caught small mammals in Thailand. PLoS Negl Trop Dis 2018 Jul; 12(7):e0006632. 54. Tam CC; Offeddu V; Anderson KB; Weg AL; Macareo LR; Ellison DW; Rangsin R; Fernandez S; Gibbons RV; Yoon IK; Simasathien S. Association between semi- quantitative microbial load and respiratory symptoms among Thai military recruits: a prospective cohort study. BMC Infect Dis 2018 Sep; 18(1):462. 55. Thongkamkoon P; Tohmee N; Morris EK; Inamnuay L; Lombardini ED. Combined fatal systemic Chlamydia sp. and Aeromonas sobria infection in juvenile Siamese crocodiles (Crocodylus siamensis). Vet Pathol 2018 Sep; 55(5):736-40. 56. Warrasak S; Euswas A; Fukuda MM; Ittiverakul M; Miller RS; Krudsood S; Ohrt C. Comparative ophthalmic assessment of patients receiving tafenoquine or chloroquine/primaquine in a randomized clinical trial for Plasmodium vivax malaria radical cure. Int Ophthalmol 2018 Sep; [Epub ahead of print]. Conference Papers 1. Ajariyakhajorn C; Hunsawong T; Mongkolsirichaikul D; Phonpakobsin T; Hussem K; Thaisomboonsuk B; Kathryn AB; Weg AL; Macareo LR; Klungthong C; Ellison DW. Factors involved in human influenza virus isolation using MDCK cell culture from South and Southeast Asian surveillance specimens. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):50. (Abstract no. 159; Session 28 - Poster Session A: Presentations and Light Lunch). 2. Akapirat S; Vasan S; Rerks-Ngarm S; Pitisutthithum P; Smith KS; Rittiroongrad S; Puangkaew J; Phogat S; Sinangil F; Michael NL; Excler J; Kim JH; Karasavvas N; O'Connell RJ; for the RV305 Study Group. Additional boost of AIDSVAX B/E further increased RV305 IgG but not IgA antibodies. Poster. 25th Conference on Retroviruses and Opportunistic Infections. Boston, USA. 4-7 March 2018. (Abstract no. 306; Poster P-C5 Vaccine Design and Testing). รายงานประจาํ ป 2561 124 ANNUAL REPORT 2018
สถาบนั วิจยั วิทยาศาสตรก ารแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 3. Alera MT. Trends and Impact of molecular and genetic diagnostic methods. 9th PAMET Visayas Regional Conference. Cebu, Philippines. 1-2 March 2018. (Technical Competence 5). 4. Anderson KB; Buddhari D; Macareo LR; Weg AL; Ellison DH; Thomas SJ; Nisalak A; Jarman RG; Yoon IK; Aldstadt J; Libraty DH; Gibbons RV; Rothman AL; Endy TP. Prior year's transmission intensity informs current risk of dengue virus infection in Thai villages. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):438. (Abstract no. 1407; Session 145 - Poster Session C: Presentations and Light Lunch). 5. Anderson KB; Simasathien S; Watanaveeradej V; Weg AL; Ellison DW; Klungthong C; Bhoomiboonchoo P; Kerdpanich P; Piboonbanakit D; Sitkittitad W; Fernandez S; Macareo LR; Yoon IK; Gibbons RV; Jarman RG. Sentinel influenza surveillance in an urban Thai military hospital, 2009-2014. Poster. 1st ASEAN Military Medicine Conference. Yangon, Myanmar. 18-21 February 2018. 6. Bodhidatta L; Pandey P; Sornsakrin S; Suksawad U; Khadka B; Shakya S; Shrestha S; Riddle MS; Mason CJ; Swierczewski B. Traveler's diarrhea and post- infectious irritable bowel syndrome in a tourist population in Nepal. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB-5255; Session 86 - Poster Session B: Presentations and Light Lunch). 7. Boonyalai N. Functional and enzymatic dissection of Plasmodium falciparum plasmepsin V. 6th International Conference on Biochemistry and Molecular Biology. 21 June 2018. 8. Boonyalai N. Methods for determining anti-malarial efficacy of Plasmodium falciparum and P. vivax isolates. Advanced Courses and Scientific Conference: Malaria Experimental Genetics. Bangkok, Thailand. 22-27 April 2018. 9. Brazeau NF; Lon C; Rao P; Parobek C; Balasubramanian S; Fukada MM; Wojnarski M; Smith P; Spring M; Juliano JJ; Carlton JM; Saunders DL; Lin JT. Plasmodium vivax whole genome sequencing to assess genetic relatedness of polyclonal relapses in Cambodia. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):425. (Abstract no. 1367; Session 137 - Malaria: Genetics and Genomics). 10. Britch S; Linthicum K; Aldridge R; Golden F; Ponlawat A; Pongsiri A; Khongtak P. Comparative efficacy of Bti, spinosad, and methoprene larvicides applied with thermal fog in a hot-humid tropical environment against Aedes and Anopheles. AMCA2018: 84th Annual Meeting. Kansas City, MO, USA. 26 February-2 March 2018. (Abstract no. 11; Session: Larval Mosquito Control I). 11. Buddhari D; Yingyong T; Ouppapong A; Kaewhiran S; Klungthong C; Macareo LR. Clinical presentations associated with influenza infection in Kamphaeng Phet province during year 2012 to 2017. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB-5322; Session 86 - Poster Session B: Presentations and Light Lunch). รายงานประจาํ ป 2561 125 ANNUAL REPORT 2018
สถาบันวจิ ยั วทิ ยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 12. Buranapraditkun S; Takata H; Sacdalan C; Manasnayakorn S; Chottanapund S; Thantiworasit P; Prueksakaew P; Benjapornpong K; Nuntapinit B; Schacker T; Robb ML; Michael NL; Ananworanich J; Trautmann L. T AND B cell alterations in peripheral blood and lymph node in acute HIV infection. Poster. 25th Conference on Retroviruses and Opportunistic Infections. Boston, USA. 4-7 March 2018. (Abstract no. 285; Poster P-C2 T and B Cell Interactions in Lymph Nodes). 13. Bystrom PV; Anderson KB; Buddhari D; Rothman AL; Weg AL; Ellison DW; Macareo LR; Endy TP. Dengue as a rural disease? Findings from a household study in Kamphaeng Phet, Thailand. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):36. (Abstract no. 112; Session 28 - Poster Session A: Presentations and Light Lunch). 14. Carrington LB; Ponlawat A; Nitatsukprasert C; Khongtak P; Sunyakumthorn P; Ege CA; Im-erbsin R; Chumpolkulwong K; Thaisomboonsuk B; Klungthong C; Ellison D; Macareo L; Simmons CP. Development of a nonhuman primate-mosquito- dengue virus infection model. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB-5235; Session 86 - Poster Session B: Presentations and Light Lunch). 15. Chaisatit C; Sai-ngam P; Kirativanich K; Kheang T; Kuntawunginn Gaywee J; Saunders D; Lon C; Jongsakul K; Spring M; Wojnarski M; Smith P; Fukuda M; Gosi P. Pattern of K13 polymorphisms among Plasmodium falciparum isolates from border areas in the Mekong subregion. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):590. (Abstract no. 1899; Session 149 - Malaria: Chemotherapy and Drug Resistance - Clinical Studies). 16. Chaitaveep N; Somsri M; Siriwatthanakul K; Thongsen N; Maza J. Trend of reemerging Neisseria gonorrhoeae infections in Thai people from 2014 to 2017. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. 17. Chinnawirotpisan P; Joonlasak K; Manasatienkij W; Klungthong C; Huang A; Mongkolsirikul D; Macareo LR; Ellison DW. Comparison of sample preparation methods for next generation sequencing of influenza A viruses. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):51. (Abstract no. 162; Session 28 - Poster Session A: Presentations and Light Lunch). 18. Chuenchitra T; Bodhidatta L; Sirisopana N; Eamsila C; Khemnu N; Wongstitwilairoong T; Gaywee J; Ellison DW; Maza JP; Macareo LR. Royal Thai Army respiratory diseases surveillance network along the border regions of Thailand: collaborative network for global health security. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. 19. Colby D; Akapirat S; Pattanachaiwit S; Trichaviroj R; Pinyakorn S; Ubolyam S; Kroon E; Sacdalan C; Chomchey N; Phanuphak N; Robb ML; Phanunphak P; Ananworanich J; de Souza M; for the RV254/SEARCH010 Study Team. How low can you go? Diagnosis of acute HIV infection at very low HIV RNA levels. Poster. 25th Conference on Retroviruses and Opportunistic Infections. Boston, USA. 4-7 March 2018. (Abstract no. 998; Poster P-S3 Early Infection and Recency Assays). รายงานประจาํ ป 2561 126 ANNUAL REPORT 2018
สถาบันวจิ ัยวิทยาศาสตรการแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 20. Colby D; Posuwan N; Sacdalan C; Intasan J; Kroon E; Wansom T; Phanuphak N; Ananworanich J; Robb ML; Phanunphak P; Poovorawan Y. Acute hepatitis A among HIV-infected Thai MSM is linked to MSM in Europe and Taiwan. Poster. 25th Conference on Retroviruses and Opportunistic Infections. Boston, USA. 4-7 March 2018. (Abstract no. 626; Poster P-K07 Non-B, Non-C Hepatitis). 21. Deutsch-Feldman M; Norris L; Wojnarski M; Brazeau N; Chaorattanakawee S; Somethy S; Saunders DL; Berjohn C; Vanachayangkul P; Spring MD; Huy R; Fukuda MM; Dysoley L; Smith P; Lon C; Juliano JJ; Lin JT. Effect of Plasmodium falciparum CRT single nucleotide polymorphism and plasmepsin 2-3 copy number increase on ex vivo piperaquine resistance in Plasmodium falciparum isolates from northwestern Cambodia, 2012-2015. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):324. (Abstract no. 1042; Session 86 - Poster Session B: Presentations and Light Lunch). 22. Dumre S; Bhandari Dumre R; Chinnawirotpisan P; Klungthong C; Shakya G; Marasini B; Shrestha S; Ghimire P; Karbwang J; Na-Bangchang K; Fernandez S; Hirayama K. Molecular epidemiological investigation revealed serotype switching observed during major dengue outbreaks (2007-2015) in Nepal. Poster. ASM Microbe 2018. Atlanta, Georgia, USA. 7-11 June 2018. (Poster no. 5; Session 282 - The Proof is in the Pattern: Epidemiology of Microbes and Poster no. 314; Session 223 - CPHM08 - Diagnostic Public Health Microbiology: Circulating, Emerging, and Reemerging Pathogens). 23. Dumre SP; Chinnawirotpisan P; Bhandari R; Klungthong C; Shakya G; Shrestha SK; Ghimire P; Yoon IK; Na-bangchang K; Hirayama K; Fernandez S. Molecular epidemiology of dengue virus serotypes in Nepal. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):41-2. (Abstract no. 130; Session 28 - Poster Session A: Presentations and Light Lunch). 24. Duncan EH; Atre T; Beck K; Dutta S; Spring M; Bergmann-Leitner ES. Quest for immune correlates of protection using a streamlined analysis approach. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):519. (Abstract no. 1671; Session 145 - Poster Session C: Presentations and Light Lunch). 25. Ellison DW; Rodpradit P; Huang A; Mongkolsirichaikul D; Wangchuk S; Shrestha SK; Velasco JM; Alera MT; Watanaveeradej V; Suwanpakdee D; Simasathien S; Weg AL; Anderson KB; Macareo LR; Klungthong C. Detection of antiviral resistance of influenza viruses in Bhutan, Nepal, the Philippines, and Thailand during 2013 to 2015. Poster. 6th International Influenza Meeting. Muenster, Germany. 2-4 September 2018. (Abstract no. 70). 26. Fansiri T; Pongsiri A; Chittham W; Kijchalao U; Phasomkusolsil S; Davidson SA; Ponlawat A. Evaluation of spatial repellent and attractant devices against malaria vectors in Thailand. Poster. 7th International Congress of the Society for Vector Ecology (SOVE): New Technology Conquering Old Vectors? Palma of Mallorca, Spain. 1-7 October 2017. (Poster: Vector control; Poster no. 115). รายงานประจําป 2561 127 ANNUAL REPORT 2018
สถาบันวิจัยวิทยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 27. Fukuda M; Smith E; Durand S; Onyango I; Spring M; Baldeviano GC; Majid E; Sriwichai S; Cummings J; Tapia L; Akala H; Gosi P; Cabezas C; Juma D; Wongararunkochakorn S; Sihuincha M; Chebon L; Kuntawunginn W; Halbach A; Forshey B; Edgel K; Saunders D; Cheruiyot A; Chuang I; Fernandes P; Lescano AG; Kamau E; Bethell D; Jongsakul K; Andagalu B. Characterization of artemisinin resistance in three continents: a multicenter trial in Peru, Kenya and Thailand in patients with uncomplicated Plasmodium falciparum malaria. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):485. (Abstract no. 1563; Session 145 - Poster Session C: Presentations and Light Lunch). 28. Gonwong S; Chuenchitra T; Khantapura P; Islam D; Ruamsap N; Mason CJ. Nationwide seroprevalence of scrub typhus among young Thai men, 2007-2008. Poster. Joint International Tropical Medicine Meeting 2017 \"Tropical Medicine 4.0: Effective Collaboration for an Impact on Global Health\". Amari Watergate Bangkok, Thailand. 6- 8 December 2017. (Poster no. 42). 29. Gosi P; Wojnarski M; Lin J; Spring M; Berjohn C; Harrison D; Sok S; Saingam P; Kirativanich K; Chaisatit C; Ittiverakul M; Buathong N; Chann S; Kuntawunginn W; Arsanok M; Rahman R; Heang V; Kong N; Chum B; Ratchmat A; Vaughn A; Prom S; Lek D; Fukuda M; Smith P; Saunders D; Lon C. Molecular detection methods to estimate Plasmodium falciparum gametocyte carriage in northwestern Cambodia. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):96. (Abstract no. 311; Session 28 - Poster Session A: Presentations and Light Lunch). 30. Gruner W; Fries A; Garrett C; Couch M; Powell M; Hanson J; Bogue A; Heang V; Ellison D; Bulimo W; Macias E. 2016-2017 DoD influenza surveillance: H3N2 predominance and appearance of influenza B amino acid deletions. Poster. ASM Microbe 2018. Atlanta, Georgia, USA. 7-11 June 2018. (Poster no. 750; Session 422 - CIV06 - Vaccines and Immunization Science: Viruses and Pertussis). 31. Hsu DC; Silsorn D; Inthawong D; Kuncharin Y; Sopanaporn J; Tayamun S; Im-Erbsin R; Ege C; Wegner M; Sunyakumthorn P; O'Connell RJ; Michael NL; Vasan S. Differential viral rebound between lymph node and colon after treatment interruption in SHIV-infected rhesus macaques. International Workshop on HIV Persistence During Therapy: Reservoirs and Eradication Strategies Workshop. Florida, Miami, USA. 12-15 December 2017. (OP 3.6; Session 3: In vitro and Animal Model Studies of HIV Persistence). 32. Hunsawong T; Lohachanakul J; Chusri S; Thaisomboonsuk B; Anderson KB; Weg AL; Macareo LR; Ellison DW. Ivermectin inhibitory effects on zika virus and chikungunya virus infection. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):46. (Abstract no. 145; Session 28 - Poster Session A: Presentations and Light Lunch). 33. Islam D; Ruamsap N; Imerbsin R; Kanellos J; Crawford JM; Swierczewski BE; Kaminski RW. Efficacy of a commercially available hyperimmune bovine colostrum product for the prevention and treatment of shigellosis in rhesus macaques. Poster. Vaccines Against Shigella and ETEC. Mexico City, Mexico. 12-14 June 2018. (Abstract no. GEN047; Session: Host Parameters and Genomics that Predict Responses to Infection and Disease). รายงานประจาํ ป 2561 128 ANNUAL REPORT 2018
สถาบันวิจยั วทิ ยาศาสตรการแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 34. Jaichapor B; Khongtak P; Nitatsukprasert C; Evans B; Ponlawat A. Field evaluation of the BG-Sentinel trap baited with different attractants for collecting Aedes albopictus in Thailand. Poster. AMCA2018: 84th Annual Meeting. Kansas City, MO, USA. 26 February-2 March 2018. (Abstract no. P-02; Poster Session: Adult Control). 35. Khantapura P; Ruamsap N; Gonwong S; Ketwalha P; Lurchachaiwong W; Serichantalergs O; Srijan A; Khemnu N; Oransathid W; Islam D; Swierczewski BE. Gastrointestinal infections in deployed US military personnel to Thailand (Cobra Gold- 2015). Poster. Joint International Tropical Medicine Meeting 2017 \"Tropical Medicine 4.0: Effective Collaboration for an Impact on Global Health\". Amari Watergate Bangkok, Thailand. 6-8 December 2017. (Poster no. 88). 36. Khantapura P; Ruamsap N; Gonwong S; Lurchachaiwong W; Lertsethtakarn P; Islam D; Swierczewski BE. Evaluation of the multiplex Luminex Gastrointestinal Pathogen Panel (xTAG GPP) testing in the diarrheic military deployed to Cobra Gold and Balikatan exercises. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):140. (Abstract no. 454; Session 28 - Poster Session A: Presentations and Light Lunch). 37. Khongtak P; Jaichapor B; Evans B; Ponlawat A. Field evaluation of CDC light traps for malaria vector surveillance in Thailand. Poster. AMCA2018: 84th Annual Meeting. Kansas City, MO, USA. 26 February-2 March 2018. (Abstract no. P-07; Poster Session: Adult Control). 38. Klungthong C; Manasatienkij W; Chinnawirotpaisan P; Macareo LR; Ellison DW; Chotpitayasunondh T; Suntarattiwong P; Olsen SJ; Kittikraisak W; Anderson KB; Rutvisuttinunt W. Co-circulation of two Yamagata-lineage clades of influenza B viruses during the 2013 and 2014 influenza seasons in Thailand and implication for vaccine effectiveness. Poster. 6th International Influenza Meeting. Muenster, Germany. 2-4 September 2018. (Abstract no. 68). 39. Kobylinski KC. Ivermectin for malaria in Southeast Asia (IMSEA Study, Thailand). American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Session 74 - Ivermectin and Mosquitoes: The Vital Role of Pharmacokinetics and Pharmacodynamics). 40. Kobylinski KC. Ivermectin mass drug administration for malaria elimination in the Greater Mekong Subregion. Joint International Tropical Medicine Meeting 2017 \"Tropical Medicine 4.0: Effective Collaboration for an Impact on Global Health\". Amari Watergate Bangkok, Thailand. 6-8 December 2017:34. (S7: Malaria Elimination Towards Eradication in 21st Century). 41. Kobylinski K; Davidson S; Phasomkusolsil S; Pantuwatana K; Jittamala P; Pukrittayakamee S; Hanboonkunupakarn B; van der Pluijm R; Hoglund R; Mukaka M; Dondorp A; Day N; White N; Tarning J. Pharmacokinetic and pharmacodynamic properties of ivermectin: ivermectin eurartesim trial, Thailand. Tracking Resistance to Artemisinin and Containment II. Bangkok, Thailand. 26 March 2018. 42. Kobylinski K. Ivermectin mass drug administration for malaria elimination in the Greater Mekong Subregion. 14th International Congress of Parasitology. Daegu, Korea. 19-24 August 2018. (Abstract no. 5302-1; Session: Anti-malaria Drug Development). รายงานประจาํ ป 2561 129 ANNUAL REPORT 2018
สถาบันวิจยั วทิ ยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 43. Krairojanan P; Thaipadungpanit J; Srilohasin P; Jones JW; Wanja EW; Davidson SA. Diversity of pathogenic Leptospira subtypes in Ranong province, Thailand. Poster. 10th International Leptospirosis Society Conference 2017 \"Science for People\". Palmerston North, New Zealand. 27 November - 1 December 2017:255. (Poster no. 44; Session 7-Group2-ID 85005). 44. Lago CB. Molecular diagnosis in clinical virology. 9th PAMET Visayas Regional Conference. Cebu, Philippines. 1-2 March 2018. (Technical Competence 2). 45. Lertsethtakarn-Ketwalha P; Sakpaisal P; Silapong S; Sherstha S; Pandey P; Liu J; Houpt E; Bodhidatta L; Swierczewski BE; Mason CJ. Quantitative analysis of diarrhea etiology in travelers' diarrhea in Nepal analysis of diarrhea etiology in travelers' diarrhea in Nepal. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):139. (Abstract no. 450; Session 28 - Poster Session A: Presentations and Light Lunch). 46. Liu J; Platts-Mills J; Rogawski E; Lima A; Kang G; Samie A; Haque R; Mduma E; Kosek M; P J; Bodhidatta L; Iqbal N; Page N; Kiwelu I; Houpt E; MAL-ED Network Investigators. Profiling of Shigella and ETEC diarrheal cases in the Mal-Ed study. Poster. ASM Microbe 2018. Atlanta, Georgia, USA. 7-11 June 2018. (Poster no. 281; Session 222 - CPHM03 - Diagnostic Bacteriology: Stool is Cool). 47. Lurchachaiwong W. Enteric etiological surveillance in acute diarrhea stool of U.S. military personnel on deployment in Thailand 2013-2017. Poster. ASM Microbe 2018. Atlanta, Georgia, USA. 7-11 June 2018. (Poster no. 421; Session 405 - AAR01 - Surveillance of AMR: Molecular Typing, Clinical Molecular Epidemiology: Multidrug Resistance in Foodborne and Waterborne Pathogens). 48. Macareo LR; Sunyakumthorn P. All about dengue viruses, vectors and animal models - Why it is complicated? (Keynote). 12th TALAS International Conference. Bangkok, Thailand. 5-7 June 2018. 49. Manasatienkij W; Chinnawirotpaisan P; Macareo L; Ellison D; Chotpitayasunondh T; Suntarattiwong P; Olsen S; Kittikraisak W; Anderson K; Rutvisuttinunt W; Klungthong C. Predominant of influenza A (H3N2) clade 3C.2a may have contributed to the low vaccine effectiveness against influenza A (H3N2) in the 2014 influenza season in Thailand. Poster. 6th International Influenza Meeting. Muenster, Germany. 2-4 September 2018. (Abstract no. 67). 50. Margulieux KR; Srijan A; Nobthai P; Ruekit S; Bodhidatta L; Pandey P; Serichantalergs O; Shrestha SK; Crawford JM; Swierczewski B. Determination of ESBL prevalence and common mechanisms in enterotoxigenic Escherichia isolated from diarrhea samples collected in Nepal during 2001-2016. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):138. (Abstract no. 447; Session 28 - Poster Session A: Presentations and Light Lunch and Session Young Investigator Award Session E). 51. Margulieux KR; Srijan A; Ruekit S; Snesrud E; Maybank R; Serichantalergs O; Kormanee R; Sukhchat P; Crawford JM; Hinkle M; McGann P; Swierczewski BE. Genomic characterization of XDR Acinetobacter baumannii isolates collected from Queen Sirikit Naval Hosp. in Thailand during 2017. Poster. ASM Microbe 2018. Atlanta, Georgia, USA. 7-11 June 2018. (Poster no. 4; Session 204 - Genomic Studies of Antibacterial Resistance and Poster no. 444; Session 228 - AAR01 - Surveillance of AMR: Molecular Typing, Clinical Molecular Epidemiology: Genomic Investigation Deciphering Antimicrobial Resistance and Lineages). รายงานประจําป 2561 130 ANNUAL REPORT 2018
สถาบนั วจิ ยั วิทยาศาสตรก ารแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 52. Margulieux KR; Srijan A; Ruekit S; Snesrud E; Ong A; Serichantalergs O; Kormanee R; Sukhchat P; Jones A; McGann P; Crawford JM; Swierczewski BE. Genomic characterization of carbapenem-resistant Escherichia coli isolated from clinical samples in Thailand. Poster. ASM Conference on Rapid Applied Microbial Next- Generation Sequencing and Bioinformatic Pipelines. Washington, DC, USA. 23-26 September 2018. (Poster no. 130; Poster Session B). 53. Monkanna N; Phasomkusolsil S; Pantuwatana K; Tawong J; Kertmanee Y; Khaosanorh S; Wanja EW; Davidson SA. Oviposition preference of Toxorhynchites splendens (Diptera: Culicidae) on the larval and pupal rearing water of Aedes aegypti and Anopheles dirus. Poster. Entomology2017. Denver, Colorado, USA. 5-8 November 2017. (Poster no. D3443; Session 360 Poster: MUVE, Behavior and Ecology). 54. Moser KA; Stucke EM; Dwivedi A; Drabek EF; Korsen S; Phillippy A; Adams M; Ouattara A; Coulibaly D; Doumbo OK; Thera MA; Saunders D; Lon C; Nyunt MM; Laufer MK; Travassos MA; Tracking Resistance to Artemisinin Collaboration (TRAC); Harrison ST; Plowe CV; Silva JC. A catalogue of structural variants in P. falciparum genomes from clinical isolates in malaria endemic regions. Poster. 7th Genomic Epidemiology of Malaria conference. Cambridge, UK. 11-13 June 2018. 55. Musich T. Granulocyte influence on adaptive immunity. HVTN Translational HIV Vaccine Early Investigator Conference. Washington, DC, USA. 17-18 May 2018. 56. Musich T; Hsu DC; Ege C; Wegner M; Silsorn D; Inthawong D; Kuncharin Y; Sopanaporn J; Tayamun S; Im-Erbsin R; Sunyakumthorn P; Michael NL; O'Connell RJ; Vasan S. Establishment of a non-human primate model of acute HIV infection as a platform to advance cure strategies to benefit HIV infected warfighters. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. 57. Nyagaya-Wojnarski B; Lon C; Sea D; Sok S; Sriwichai S; Chann S; Hom S; Ly S; Sok C; Nou S; Oung P; Kong N; Pheap V; Thay K; Dao V; Kuntawunginn W; Boonchan T; Feldman M; Buathong N; Ittiverakul M; Rekol H; Dysoley L; Spring M; Smith P; Wojnarski M; Fukuda M. Evaluation of the CareStart. Glucose-6- phosphate dehydrogenase (G6PD) rapid diagnostic test at community and health center level in Cambodia. George Washington University Conference. Washington, DC, USA. 18 May 2018. 58. Nyagaya-Wojnarski B; Lon C; Sea D; Sok S; Sriwichai S; Chann S; Hom S; Ly S; Sok C; Nou S; Oung P; Kong N; Pheap V; Thay K; Dao V; Kuntawunginn W; Boonchan T; Feldman M; Buathong N; Ittiverakul M; Rekol H; Dysoley L; Spring M; Smith P; Wojnarski M; Fukuda M. Evaluation of the CareStartTM glucose-6- phosphate dehydrogenase (G6PD) rapid diagnostic test at community and health center level, Cambodia. Poster. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. 59. O'Connell RJ; Schuetz A; Sacdalan C; Ratnaratorn N; Phuang-Ngern Y; Kroon E; Rerknimitr R; Chottanapund S; Hsu D; Wansom T; Michael NL; Robb ML; Ananworanich J; Vasan S; for the RV304 Study Group. Assessing biologic risk for HIV transmission in transgender women, an important key risk group. Poster. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. (Track: Total Force Health; Breakout Session: Medicine and Surgery). 60. Pathawong N; Puntim P; Fansiri T; Nitatsukprasert C; Pongsiri A; Davidson SA; Ponlawat A. Effect of pyriproxyfen on the fecundity of Aedes aegypti. Poster. 7th International Congress of the Society for Vector Ecology (SOVE): New Technology Conquering Old Vectors? Palma of Mallorca, Spain. 1-7 October 2017. (Poster: Vector Control; Poster no. 111). รายงานประจาํ ป 2561 131 ANNUAL REPORT 2018
สถาบันวจิ ยั วทิ ยาศาสตรก ารแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 61. Phasomkusolsil S; Monkanna N; Pantuwatana K; Tawong J; Kertmanee Y; Khaosanorh S; Wanja EW; Davidson SA. Laboratory studies on oviposition preference of Toxorhynchites splendens in the presence of conspecific and Aedes aegypti larvae. Poster. Entomology2017. Denver, Colorado, USA. 5-8 November 2017. (Poster no. D3442; Session 360 Poster: MUVE, Behavior and Ecology). 62. Pinilla YT; Lopes S; Sampaio V; Andrade F; Vasquez G; Escobedo K; Lopes V; Melo GC; Velasquez C; Kobilynski K; Stoops C; Guerra MG; Lacerda MV; Pimenta PF; Monteiro WM. Ivermectin inhibits the development of Plasmodium vivax in Anopheles aquasalis and Anopheles darlingi. Poster. 7th International Congress of the Society for Vector Ecology (SOVE): New Technology Conquering Old Vectors? Palma of Mallorca, Spain. 1-7 October 2017. (Poster: Vector-Pathogen interactions; Poster no. 143). 63. Pitisuttithum P; Nitayaphan S; Chariyalertsak S; Akapirat S; Schuetz A; Wieczorek L; Polonis VR; Eller M; Phogat S; Sinangil F; Dhitavat J; Phonrat B; Kaewkungwal J; Smith KS; Robb ML; Michael NL; Excler J; Kim JH; O'Connell RJ; Vasan S; on behalf of the RV306 Study Team. Safety and immune responses of additional boosting of the RV144 ALVACr-HIV/AIDSVAXr B/E vaccine prime and boost regimen. 3rd International Conference on Vaccines Research and Development. Washington, DC, USA. 13-15 November 2017. 64. Platts-Mills J; Liu J; Gratz J; Operario D; Rogawski E; McMurry T; Nshama R; Maro A; Mduma E; Samie A; Page N; Praharaj I; Kang G; Mason C; Bodhidatta L; Lertsehtakarn P; Shakoor S; Kabir F; Qureshi S; Iqbal N; Haque R; Taniuchi M; Khan S; Kosek M; Salas M; Havt A; Maciel I; Leite J; Lima A; Houpt ER. A comprehensive reanalysis of the etiology of community diarrhea in the MALED study using quantitative PCR. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB-5259; Session 86 - Poster Session B: Presentations and Light Lunch). 65. Platts-Mills J; Liu J; Rogawski E; McMurry T; Nshama R; Mujaga B; Maciel I; Havt A; Qureshi S; Kabir F; Islam MO; Praharaj I; Rajendiran R; Salas M; Yori P; Taniuchi M; Gratz J; Operario D; Lertsethtakarn P; Murei A; Aziz F; Kalam A; Rizvi I; Sakpaisal P; Silapong S; Seidman J; Lang D; Gottlieb M; Lima A; Samie A; Bessong P; Mduma E; Leite JP; Bodhidatta L; Mason C; Page N; Kiwelu I; Iqbal N; Ahmed T; Bhutta Z; Kang G; Haque R; Kosek M; Houpt E. Etiology, burden, and characteristics of diarrhea in children in low-resource settings using quantitative molecular diagnostics: Results from the MAL-ED study. Vaccines Against Shigella and ETEC. Mexico City, Mexico. 12-14 June 2018. (Abstract no. GB069; Session: Global Burden of Disease, Epidemiology, and Health Economics). 66. Pongsiri A; Nitatsukprasert C; Phasomkusolsil S; Schuster AL; McCardle PW; Ponlawat A. Evaluation of pyriproxyfen and spinosad treated device against Aedes aegypti (Diptera: Culicidae). Poster. 7th International Congress of the Society for Vector Ecology (SOVE): New Technology Conquering Old Vectors? Palma of Mallorca, Spain. 1-7 October 2017. (Poster: Vector Control; Poster no. 114). 67. Rogawski E; Platts-Mills J; Liu J; Nshama R; Mujaga B; Araujo I; Havt A; Qureshi S; Kabir F; Islam MO; Praharaj I; Rajendiran R; Yori P; Salas M; Taniuchi M; Gratz J; Operario D; Lertsethtakarn P; Murei A; Aziz F; Kalam A; Rizvi I; Sakpaisal P; Silapong S; Seidman J; Lang D; Gottlieb M; Lima A; Samie A; Bessong P; Mduma E; Liete JP; Bodhidatta L; Mason C; Page N; Kiwelu I; Iqbal N; Ahmed T; Bhutta Z; Kang G; Haque R; Kosek M; Houpt E. Impact of enteropathogen infection on linear growth using quantitative molecular diagnostics: รายงานประจําป 2561 132 ANNUAL REPORT 2018
สถาบนั วิจยั วทิ ยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES Results from the MAL-ED study. Vaccines Against Shigella and ETEC. Mexico City, Mexico. 12-14 June 2018. (Abstract no. GB065; Session: Global Burden of Disease, Epidemiology, and Health Economics). 68. Rouhani S; Olortegui MP; Yori PP; Salas MS; Rengifo DT; Mondal D; Bodhidatta L; Platts-Mills J; Samie A; Kabir F; Lima A; Babji S; Mason CJ; Kalam A; Bessong P; Ahmed T; Mduma E; Bhutta ZA; Lima I; Ramdass R; Moulton LH; Lang D; George A; Zaidi AKM; Kang G; Houpt ER; Kosek MN. Astrovirus infection, diarrhea and immunity in eight countries: Results from the MAL-ED study. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB-5326; Session 86 - Poster Session B: Presentations and Light Lunch). 69. Ruamsap N; Khantapura P; Gonwong S; Islam D; Chuenchitra T; Jones JW. A serologic survey using ELISA to determine the prevalence of Q fever among Royal Thai Army recruits, 2012. Poster. Joint International Tropical Medicine Meeting 2017 \"Tropical Medicine 4.0: Effective Collaboration for an Impact on Global Health\". Amari Watergate Bangkok, Thailand. 6-8 December 2017. (Poster no. 44). 70. Ruamsap N; Imerbsin R; Kaminski RW; Swierczewski BE; Crawford JM; Islam D. Efficacy of a non-antibiotic based drug, and a passive immunotherapeutic agent, for the treatment of diarrhea in rhesus monkey model. Poster. 2018 Global Vaccine and Immunization Research Forum. Bangkok, Thailand. 20-22 March 2018. 71. Ruamsap N; Khantapura P; Gonwong S; Khemnu N; Chuenchitra T; Islam D; Swierczewski BE; Mason CJ. Absence of H5N1 avian influenza exposure in young Thai men residing in endemic areas. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. 72. Ruamsap N; Khantapura P; Gonwong S; Khemnu N; Chuenchitra T; Islam D; Swierczewski BE; Mason CJ. Avian influenza exposure in young Thai males from Suphanburi province of Thailand. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):50-1. (Abstract no. 160; Session 28 - Poster Session A: Presentations and Light Lunch). 73. Ruekit S; Srijan A; Margulieux KR; McGann P; Serichantalergs O; Kormanee R; Sukhchat P; Crawford JM; Swierczewski BE. Prevalence and molecular characterization of multidrug-resistant ESKAPE pathogens isolated from patients at a naval hospital in Thailand: 2016-2018. Poster. 9th International Conference on Emerging Infectious Diseases. Zurich, Switzerland. 27-28 August 2018. J AIDS Clin Res 2018; 9:85-6. 74. Ruekit S; Srijan A; Serichantalergs O; Margulieux KR; Kormanee R; Sukhchat P; Sriyabhaya J; McGann P; Hinkle M; Crawford JM; Swierczewski BE. Molecular characterization of extended-spectrum beta-lactamase and carbapenem-resistant genes present in multidrug resistant clinical isolates in Thailand. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB-5266; Session 86 - Poster Session B: Presentations and Light Lunch). 75. Ruksasiri S; Bodhidatta L; Serichantalergs O; Mason CM; Tribuddharata C. Integron analysis of multidrug-resistant diarrheagenic E. coli isolates from travelers in Thailand. Poster. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. รายงานประจาํ ป 2561 133 ANNUAL REPORT 2018
สถาบนั วิจยั วิทยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 76. Rungrojn A; Korkusol A; Tippayachai B; Leepitakrat S; Davidson SA; Takhampunya R. Improvement of sequences based typing of Leptospira species using single gene versus multigene sequence analysis. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB-5395; Session 145 - Poster Session C: Presentations and Light Lunch). 77. Schuetz A; Akapirat S; Chuenarom W; Pitisutthithum P; Nitayaphan S; Chariyalertsak S; Kaewkungwal J; Phogat S; Sinangil F; Robb ML; Michael NL; Kim JH; de Souza M; O'Connell RJ; Vasan S. Increase in B cell responses upon late boost strategies of Alvac-HIV/AIDSVAX B/E. Poster. 25th Conference on Retroviruses and Opportunistic Infections. Boston, USA. 4-7 March 2018. (Abstract no. 306; Poster P-C5 Vaccine Design and Testing). 78. Schuetz A. Inflammatory mucosal macrophages and plasmacytoid dendritic cells are expanded in HIV-infected Thais on suppressive ART and are associated with Proteobacteria abundance in the GI tract. Poster. 1st HIV Mucosal Systems Meeting. California, USA. 29 April-2 May 2018. 79. Schuetz A. Mucosal immunology in acute HIV infection and cure studies in Thailand. U.S.-Japan Cooperative Medical Sciences Program (USJCMSP) 20th International Conference on Emerging Infectious Diseases in the Pacific Rim. Shenzhen, China. 10-11 January 2018. (AIDS and Immunology Joint Panel; Session 4: Mucosal Immunology and Vaccine Responses). 80. Schuetz A. Mucosal immunology in acute HIV infection and cure studies in Thailand. 1st HIV Mucosal Systems Meeting. California, USA. 29 April-2 May 2018. (Session 6: Next Steps). 81. Schuetz A; Sacdalan C; Ratnaratorn N; Phuang-Ngern Y; Kroon E; Rerknimitr R; Chottanapund S; Hsu DC; Wansom T; Michael NL; Robb ML; O'Connell RJ; Ananworanich J; Vasan S. Assessing biologic risk for HIV transmission in transgender women. Poster. 25th Conference on Retroviruses and Opportunistic Infections. Boston, USA. 4-7 March 2018. (Abstract no. 204; Poster P-B1 HIV Transmission and Disease Progression). 82. Silapong S; Lertsethtakarn-Ketwalha P; Sherstha S; Pandey P; Bodhidatta L; Mason C; Swierczewski B. Detection Cryptosporidium and Cyclospora of digital droplet PCR (ddPCR) from travelers' diarrhea and Nepalese stool samples. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):139. (Abstract no. 451; Session 28 - Poster Session A: Presentations and Light Lunch). 83. Sirikajornpan K; Thaisomboonsuk B; Nisalak A; Klungthong C; Hunsawong T; Poolpanichupatam Y; Kaneechit W; Imerbsin R; Buathong R; Chusri S; Theresa M; Watanaveeradej V; Buddhari D; Fernandez S; Yoon IK; Weg AL; Macareo LR; Ellison DW. Development and evaluation of a zika virus IgM MAC-ELISA compared to two commercially available ZIKV-IgM ELISA Kits for serological diagnosis of zika virus infection. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB-5074; Session 28 - Poster Session A: Presentations and Light Lunch). 84. Smith KS; Sriplienchan S; Kitsiripornchai S; Charuthamrong P; Kroon E; Vasan S; O'Connell RJ; Nitayaphan S; Robb M. Monitoring and evaluating community stakeholder engagement strategies in populations at high risk of HIV in Pattaya, Thailand. Poster. Drug Information Association 2018, Global Annual Meeting. Boston, MA, USA. 24-28 June 2018. รายงานประจาํ ป 2561 134 ANNUAL REPORT 2018
สถาบันวจิ ัยวิทยาศาสตรการแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 85. Smith PL. The US Army's malaria research and development program: an overview. 1st ASEAN Military Medicine Conference. Yangon, Myanmar. 18-21 February 2018. 86. Spring M; Lon C; Wojnarski M; Sok S; Sea D; Chann S; Sriwichai S; Nou S; Ittiverakul M; Kuntawunginn W; Arsanok M; Vanachayangkul P; So M; Prom S; Huy R; Fukuda M; Saunders D; Smith P. Prevalence of CYP2D6 polymorphisms in a Cambodian population and relationship to Plasmodium vivax recurrence rate and hemolytic toxicity. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):296. (Abstract no. 954; Session 86 - Poster Session B: Presentations and Light Lunch). 87. Srijan A; Lurchachaiwong W; Wongstitwilairoong B; Bodhidatta L; Mason C; Swierczewski B. Prevalence and antimicrobial resistance of non-typhoid Salmonella in US military personnel to Thailand, 1988-2013. Poster. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. 88. Srijan A; Margulieux KR; Ruekit S; McGann P; Snesrud E; Maybank R; Serichantalergs O; Kormanee R; Sukhchat P; Hinkle M; Crawford JM; Swierczewski BE. Genetic characterization and epidemiology of multidrug-resistant gram-negative ESKAPE pathogens, including extremely drug-resistant strains at a naval hospital in Thailand. Poster. 9th International Conference on Emerging Infectious Diseases. Zurich, Switzerland. 27-28 August 2018. J AIDS Clin Res 2018; 9:83-4. 89. Srijan A; Margulieux KR; Ruekit S; Snesrud E; Maybank R; Serichantalergs O; Kormanee R; Sukhchat P; Sriyabhaya J; McGann P; Hinkle M; Crawford JM; Swierczewski BE. Non-clonal Klebsiella pneumoniae strains carrying multiple antimicrobial resistance genes including plasmid-mediated mcr-1 isolated from clinical samples in Thailand. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB- 5020; Session 28 - Poster Session A: Presentations and Light Lunch). 90. Srisawat N; Tachaboon S; Krairojananan P. The correlation of leptospiremia and organ dysfunction in leptospirosis: result from Thai-Lepto AKI study. 10th International Leptospirosis Society Conference 2017 \"Science for People\". Palmerston North, New Zealand. 27 November - 1 December 2017:128. (Session 4-Oral-ID 85057). 91. Sunyakumthorn P; Anantatat T; Im-erbsin R; Sumonwiriya M; Chumpolkulwong K; Wongwairot S; Vongsawan A; Dunachie SJ; Wegner MD; Ege CA; Jones JW; Swierczewski BE; Richards AL; PJ NP; Mason CJ; Paris DH. Immunopathological study of scrub typhus in rhesus macaques following intradermal inoculation of Orientia tsutsugamushi Karp and Gilliam strains. Joint International Tropical Medicine Meeting 2017 \"Tropical Medicine 4.0: Effective Collaboration for an Impact on Global Health\". Amari Watergate Bangkok, Thailand. 6-8 December 2017:61. (S11: Free paper II: Tropical Medicine I). 92. Sunyakumthorn P; Sumonwiriya M; Im-erbsin R; Anantatat T; Chumpolkulwong K; Wongwairot S; Dunachie SJ; Lombardini ED; Burke RL; Wegner MD; Ege CA; Jones JW; Swierczewski BE; Richards AL; Day NP; Mason CJ; Paris DH. Immune response to scrub typhus in rhesus macaques by intradermal inoculation of Orientia tsutsugamushi. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB-5269; Session 86 - Poster Session B: Presentations and Light Lunch). รายงานประจําป 2561 135 ANNUAL REPORT 2018
สถาบนั วจิ ัยวิทยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 93. Ta-aksorn W; Kodchakorn C; Smith PL; Vanachayangkul P; Wojnarski M. UPLC- MSMS method development and validation of atovaquone in human plasma for pharmacokinetic study. Poster. Joint International Tropical Medicine Meeting 2017 \"Tropical Medicine 4.0: Effective Collaboration for an Impact on Global Health\". Amari Watergate Bangkok, Thailand. 6-8 December 2017:111. (Poster no. 104;S13: Turbo Talk II). 94. Tachaboon S; Krairojananan P; Sirsawat N. Study of diagnostic accuracy comparison of 3 diagnostic tests for early diagnosis human leptospirosis: result from Thai-Lepto AKI study. 10th International Leptospirosis Society Conference 2017 \"Science for People\". Palmerston North, New Zealand. 27 November - 1 December 2017:122. (Session 4-Oral- ID 85054). 95. Takhampunya R; Klangthong K; Promsthaporn S; Leepitakrat S; Kosoy M; Silas DA. Prevalence and diversity of Bartonella species in ectoparasites from wild-caught rodents and domestic animals in the northern and northeastern regions of Thailand. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB-5396; Session 145 - Poster Session C: Presentations and Light Lunch). 96. Takhampunya R. Prevalence and diversity of Bartonella species in ectoparasites from wild-caught rodents and domestic animals in the northern and northeastern regions of Thailand. Poster. International Conference on Zoology, Microbiology Medical Parasitology. Chicago, Illinois, USA. 30 October - 1 November 2017. (Poster no. ZM-03). 97. Tawong J; Phasomkusolsil S; Pantuwatana K; Monkanna N; Kertmanee Y; Khaosanorh S; Wanja EW; Davidson SA. Effects of human and rhesus macaque blood meal sources for optimization of reproduction and adult survivorship of mosquitoes under laboratory conditions. Poster. Joint International Tropical Medicine Meeting 2017 \"Tropical Medicine 4.0: Effective Collaboration for an Impact on Global Health\". Amari Watergate Bangkok, Thailand. 6-8 December 2017. (Poster no. 100). 98. Thaisomboonsuk B. How reliable are laboratory diagnosis methods for arboviral diseases? (Development of rapid diagnosis tests?). 3rd Asia Dengue Summit. Kuala Lumpur, Malaysia. 5-6 July 2018. (Session 2: Diagnostics and Dengue Management; Symposium 3: Laboratory Diagnosis of Dengue). 99. Thamnurak C; Chattrakarn S; Praditpol C; Heang V; Chum B; Ratchmat A; Vaughn A; Kong N; Lek D; Prom S; Sok S; Kuntawunginn W; Chann S; lttiverakul M; Chaorattanakawee S; Gosi P; Boonyalai N; Vanachayangkul P; Spring M; Fukuda M; Lon C; Smith P; Saunders D; Wojnarski M; Ly S; Sok C. Variability in antimalarial drug sensitivities across regions in Cambodia may pose unique challenges to National Malaria Program. Poster. Joint International Tropical Medicine Meeting 2017 \"Tropical Medicine 4.0: Effective Collaboration for an Impact on Global Health\". Amari Watergate Bangkok, Thailand. 6-8 December 2017:71. (Poster no. 64; S13: Turbo Talk II). 100. Trichavaroj R; Pattanachaiwit S; Ubolyam S; Sawangsinth P; Intasan J; Kroon E; Colby D; O'Connell RJ; Vasan S; Phanuphak P; Phanuphak N; Ananworanich J; de Souza M; Akapirat S; for the SEARCH019/RV409 and SEARCH022/RV411 Study Groups. HIV serology following treatment interruption in very early treated people. Poster. 25th Conference on Retroviruses and Opportunistic Infections. Boston, USA. 4-7 March 2018. (Abstract no. 343; Poster P-D03 HIV Reservoirs in Acute Infection). รายงานประจาํ ป 2561 136 ANNUAL REPORT 2018
สถาบันวจิ ัยวิทยาศาสตรการแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 101. Ubalee R; Lorena MJ; Wanasit S; Sootichote R; Kayha A; Phunkitchar V; Rochanarutaiprida N; Buadok W; Im-erbsin R; Kobylinski K; Davidson SA. Inhibition of P. cynomolgi infection in vector mosquitoes by genetic engineered symbiotic bacteria. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. (Abstract no. LB- 5061; Session 28 - Poster Session A: Presentations and Light Lunch). 102. Valderama MT. Research opportunities for career advancement and growth for Medical Technologists: AFRIMS experience. 53rd Philippine Association of Medical Technologists Annual Convention \"Raising the Gold Standard for World-class Medical Technologists\". Marriott Hotel, Newport City, Pasay City, Philippines. 20-23 November 2017. 103. Velasco JM. AFRIMS collaborative projects in Manila, Philippines: research updates. 53rd Philippine Association of Medical Technologists Annual Convention \"Raising the Gold Standard for World-class Medical Technologists\". Marriott Hotel, Newport City, Pasay City, Philippines. 20-23 November 2017. 104. Velasco JM; Gaurano N; Diones PC; Valderama MT; Nogrado K; Alera MT; Chua D Jr; Ellison D; Weg A; Macareo L; Swierczewski B. Mycobacterium tuberculosis prevalence in a military population. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):581. (Abstract no. 1871; Session 145 - Poster Session C: Presentations and Light Lunch). 105. Velasco JM; Valderama MT; Lopez MN; Chua D Jr; Alera MT; Diones PC; Nogrado K; Develos M; Sanchez E; Ellison D; Weg A; Macareo L. Dengue prevalence in a military vs civilian population. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):252. (Abstract no. 807; Session 86 - Poster Session B: Presentations and Light Lunch). 106. Walker KD; Rowland T; McDermott E; Jin-Clark Y; Ubalee R; Kayha A; Buadok W; Phunkitchar V; Lopez J; Davidson S; Garver L. Ability of commercially available human rapid diagnostic tests (RDTs) to detect dengue and malaria in arthropod vectors. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97 (5 suppl):462. (Abstract no. 1490; Session 145 - Poster Session C: Presentations and Light Lunch). 107. Wangchuck S; Phonpakobsin T; Thapa B; Zangmo S; Chinnawirotpisan P; Manasatienkij W; Thaisomboonsuk B; Ajariyakhajorn C; Mongkolsirichaikul D; Macareo LR; Weg AL; Anderson KB; Ellison DW; Klungthong C. Phylogenetic analysis of influenza viruses in Bhutan during 2008-2017. Poster. 6th International Influenza Meeting. Muenster, Germany. 2-4 September 2018. (Abstract no. 69). 108. Wassanarungroj P; Nobthai P; Ruekit S; Srijan A; Sukhchat P; Kormanee R; Margulieux K; Serichantalergs O; Crawford J; Swierczewski B. Molecular characterization of methicillin resistant Staphylococcus aureus strains isolated from Queen Sirikit Naval Hospital, Thailand. Poster. ASM Microbe 2018. Atlanta, Georgia, USA. 7-11 June 2018. (Poster no. 429; Session 050 - AAR01 - Surveillance of AMR: Molecular Typing, Clinical Molecular Epidemiology: Molecular Typing and Evolution of Resistance in Staphylococci, Enterococci and Streptococci). 109. Weg A. \"Winter is Coming\": Prevention, management, and treatment of freezing and non-freezing cold injuries. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. รายงานประจาํ ป 2561 137 ANNUAL REPORT 2018
สถาบนั วจิ ัยวทิ ยาศาสตรการแพทยทหาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 110. Weg AL. Pre/post deployment medical screenings. Clinical Laboratory and Biopreparedness Subject Matter Expert Exchange (SMEE). Dhaka, Bangladesh. 5-8 August 2018. 111. Wegner M; Blacksell S; Lombardini E; Yingst S. A Model for veterinary laboratory capacity building for the early detection of zoonotic diseases, Laos PDR. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. (TRACK Breakout Session: 5 Preventive Medicine, b Zoonotic Diseases-Veterinary Health). 112. Wojnarski M. Emergence of \"superbug\" multi-drug resistant malaria in Southeast Asia: Implications on treatment. Subject Matter Expert Exchange (SMEE) on Military Medical Epidemiology for Emergency Response Among Military Medical Services of ASEAN Member State. Samut Songkhram Province, Thailand. 30 July - 3 August 2018. 113. Wojnarski M; Gosi P; Saingam P; Spring M; Lin J; Chaisatit C; Kirativanich K; Vanachayangkul P; Boonyalai N; Kuntawunginn W; Chattrakarn S; Thamnurak C; Soveasna K; Martin N; Jongsakul K; Takala-Harrison S; Rekol H; Dysoley L; Sok S; Satharath P; Saly K; Fukuda M; Saunders D; Smith P; Lon C. The emergence of \"superbug\" multi-drug resistant malaria in Southeast Asia and implications on malaria treatment. Poster. Military conference MHSRS. Kissimmee, Florida, USA. 20-23 August 2018. 114. Wojnarski M; Gosi P; Waltmann A; Lin J; Berjohn C; Spring M; Chaorattanakawee S; Boonyalai N; Vanachayangkul P; Harrison D; Sok S; Ittiverakul M; Buathong N; Chann S; Kuntawunginn W; Heang V; Kong N; Chum B; Ratchmat A; Vaughn A; Prom S; Lek D; Smith P; Fukuda M; Saunders D; Lon C. Current state of malarone resistance in Cambodia and its implications on the treatment of Plasmodium falciparum in Southeast Asia. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):404. (Abstract no. 1299; Session 91 - Malaria: Chemotherapy and Drug Resistance - Molecular Biology). 115. Wojnarski M; Gosi P; Waltmann A; lin J; Spring M; Berjohn C; Chaorattanakawee S; Boonyalai N; Vanachayangkul P; Harrison D; Sok S; lttiverakul M; Buathong N; Chann S; Kuntawunginn W; Heang V; Kong N; Chum B; Ratchmat A; Vaughn A; Prom S; Lek D; Smith P; Fukuda M; Saunders D; Lon C. Most patients in Cambodia with treatment failure post atovaquone-proguanil lack cytb mutations in Y268 locus by Sanger sequencing. Poster. Joint International Tropical Medicine Meeting 2017 \"Tropical Medicine 4.0: Effective Collaboration for an Impact on Global Health\". Amari Watergate Bangkok, Thailand. 6-8 December 2017:112. (Poster no. 105; S13: Turbo Talk II). 116. Wojnarski M; Lon C; Kuntawunginn W; Spring M; Berjohn C; Harrison D; Sok S; Ittiverakul M; Buathong N; Chann S; Heang V; Kong N; Chum B; Ratchmat A; Vaughn A; Prom S; Lek D; Smith P; Fukuda M; Saunders D. The performance of G6PD rapid diagnostic tests in Cambodia and implications for primaquine therapy. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):498. (Abstract no. 1605; Session 145 - Poster Session C: Presentations and Light Lunch). 117. Wojnarski M; Lon C; Sok S; Spring M; Lin J; Gosi P; Vanachayangkul P; Boonyalai N; Chaisatit C; Kirativanich K; Saingam P; Soveasna K; Rekol H; Shannon T; Martin N; Dysoley L; Satharath P; Saly K; Fukuda M; Smith P. Rising challenges for the Asia Pacific region's militaries in the treatment of \"superbug\" multi- drug resistant malaria. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. รายงานประจาํ ป 2561 138 ANNUAL REPORT 2018
สถาบนั วิจยั วทิ ยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 118. Wojnarski M; Sok S; Lon C; Chann S; Bun R; Spring M; Boonchan T; Gosi P; Soveasna K; Buathong N; Ittiverakul M; Sriwichai S; Kuntawunginn W; Rekol H; Sinoun M; Thay K; So M; Lin J; Manning J; Satharath P; Saly K; Saunders D; Smith P; Fukuda M. The impact of primaquine (PQ) deployment and insecticide treated uniforms on Plasmodium vivax incidence in a pilot malaria elimination study in Cambodia. Poster. American Society of Tropical Medicine and Hygiene 66th Annual Meeting. Baltimore, Maryland, USA. 5-9 November 2017. Am J Trop Med Hyg 2017; 97(5 suppl):310. (Abstract no. 998; Session 86 - Poster Session B: Presentations and Light Lunch). 119. Wongstitwilairoong T; Shrestha SK; Velasco JM; Lon C; Chuenchitra T; Buddhari D; Dorji T; Smith PL; Macareo LR; Maza JP. Rising to the global health challenges of AFRIMS influenza surveillance. Poster. Asia Pacific Military Health Exchange. Xi'an, China. 17-21 September 2018. Other Presentations, Trainings & Lectures 1. Anderson K. Zika in Asia: unique challenges, unique opportunities. 2nd Regional Workshop on Zika. Phuket, Thailand. 18-19 April 2018. 2. Chinnawirotpisan P. Phylogenetic tree construction and other specific molecular characterization of flaviviruses. From ABC to NGS in Virology: Molecular Diagnosis and Genetic Characterization of Influenza and Other Viruses Workshop. Chulalongkorn University, Bangkok, Thailand. 24-26 July 2018. 3. Crawnford J. AMR collaborations: Queen Sirikit Naval Hospital, Thailand; Military Region 5 Hospital, Cambodia. Antibiotic Awareness Week. National Maternal and Children Health Center, Cambodia. 16-17 November 2017. 4. Klungthong C. Flaviviruses: genetic difference among flaviviruses, update on molecular epidemiology and evolution of flaviviruses. From ABC to NGS in Virology: Molecular Diagnosis and Genetic Characterization of Influenza and Other Viruses Workshop. Chulalongkorn University, Bangkok, Thailand. 24-26 July 2018. 5. Klungthong C. NGS for pathogen identification at AFRIMS. From ABC to NGS in Virology: Molecular Diagnosis and Genetic Characterization of Influenza and Other Viruses Workshop. Chulalongkorn University, Bangkok, Thailand. 24-26 July 2018. 6. Kobylinski K; Davidson S; Ubalee R; Phasomkusolsil S; Roth A; Adams J; Foy B; Jittamala P; Pukrittayakamee S; Hanboonkunupakarn B; Pluijm R; Mukaka M; Wattanakul T; Tarning J; Dondorp A; Day N; White N. Ivermectin mass drug administration for malaria elimination in the Greater Mekong Subregion. Johns Hopkins University. Baltimore, MD, USA. 31 July 2018. 7. Kobylinski K; Davidson S; Ubalee R; Phasomkusolsil S; Roth A; Adams J; Foy B; Jittamala P; Pukrittayakamee S; Hanboonkunupakarn B; Pluijm R; Mukaka M; Wattanakul T; Winterberg M; Hoglund R; Tarning J; Dondorp A; Day N; White N. Ivermectin mass drug administration to suppress outdoor malaria transmission. Asia Pacific Malaria Elimination Network - Vector Control Working Group. Bangkok, Thailand. 3-5 September 2018. 8. Prasit T; Charuthamrong P. Experiences, obstacles and resolved of counseling to participants in ECHO Center (ECHO or Early Capture HIV Cohort). Counselling Sharing Training. Vaccine Trial Center, Faculty of Tropical Medicine, Mahidol University, Rose Garden Resort, Nakhompathom. 5 April 2018. รายงานประจาํ ป 2561 139 ANNUAL REPORT 2018
สถาบนั วิจยั วทิ ยาศาสตรก ารแพทยท หาร ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES 9. Smith KS. Good participatory practice lessons learned from ECHO center, Pattaya, Thailand. AVAC Good Participatory Practice (GPP) Online Training Course - Web Conference. 16 November 2017. 10. Spring MD. The contribution of humoral immunity to clinical outcomes of Plasmodium falciparum infection in Cambodia. Giving a talk at University of Maryland. Baltimore, MD, USA. 25 June 2018. 11. Sukpanichnant B. Human subjects protection. Pharmacy Faculty, Silpakorn University, Thailand. 29 March 2018. 12. Sukpanichnant B. Safety issues and reporting. Good Clinical Research Practice: GCP Training. Faculty of Medicine Siriraj Hospital, Bangkok, Thailand. 7-8 December 2017. 13. Vasan S. Current state of HIV vaccine development. AVAC HIV Vaccine Awareness Day 2018 Webinar. 17 May 2018. 14. Vasan S. RV305, RV306, RV308: objectives and trial designs. Celebration of 25 Years of US-Thai Partnership on HIV Vaccine Development. Thailand. 9 May 2018. 15. Velasco JM; Diones PC. Collaborative projects with the Armed Forces of the Philippines. Meeting with AFP and AFRIMS. V. Luna Medical Center, Quezon City, Philippines. 3 May 2018. 16. Velasco JM. Impact of temperature on infectious disease transmission. Der Forscher - ECCRI Lecture Series 4. Environmental and Climate Change Research Institute, De La Salle Araneta University, Malabon City, Philippines. 13 March 2018. รายงานประจาํ ป 2561 140 ANNUAL REPORT 2018
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