HPLC Catalogue

HPLC/UHPLC Columns Selectivity Comparison of Reversed Phase Columns Inertsil ODS-80A InertSustainSwift C8 7 HPLC/UHPLC Columns 2 7 1 2 4 6, 5 3 4 56 13 10 0 0 10 20 30 40 Time(min) 20 Time(min) Inertsil C8-4 7 20 20 InertSustain C8 4 7 2 5,6 20 65 13 4 20 2 10 20 13 Time(min) 20 0 10 Time(min) 0 Inertsil C8-3 Inertsil WP300 C8 7 2 7 2 5,6 13 5,6 13 4 0 4 20 0 10 Time(min) 10 Time(min) Inertsil WP300 C4 7 Inertsil C4 7 2 45 6 7 20 1,2 4 6 5 10 13 20 3 Time(min) 0 10 0 7 Time(min) InertSustain Phenyl InertSustain Phenylhexyl 2 5 2 46 5 13 13 46 10 Time(min) 0 10 0 5,7 Time(min) Inertsil Ph-3 5,7 Inertsil Ph 46 2 46 13 2 13 0 10 20 0 10 Time(min) Time(min) InertSustain Cyano 7 2 13 46 5 0 10 20 Time(min) HPLC Column Catalog Ex 18 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-7 LIFE SCIENCE

Colonnes HPLC/UHPLC InertSustain® C18 Colonnes HPLC/UHPLC WWiiddeeppHHcocmopmatpibaitliitbyiwlitityhwLointhg CLoolunmgnCLoifleutmimne Lifetime % Initial N 100 Purging Conditions Physical Properties 80 Column : 4.6 mm I.D.× 150 mm 5 μm Eluent : A) 50 mM Triethylamine (pH 10.0) Silica : ES Silica Gel 60 Flow Rate B) CH3OH Col. Temp. A / B = 70 / 30. V/V. Particle Size : 2 μm, 3 μm, 5 μm Tailing developed after 72 hours : 1.0 mL / min on XTerra MS C18 and Gemini-NX C18. : 50°C Surface Area : 350 m2/g 40 Analytical Conditions Pore Size : 100 Å InertSustain C18 Column : 4.6 mm I.D.× 150 mm 5 μm Eluent :CH3CN / H2O (65 / 35) Pore Volume : 0.85 mL/g 20 XTerra MS C18 (Waters) Flow Rate :1.0 mL / min Gemini-NX C18 (Phenomenex) Col. Temp. :40°C Bonded Phase : Octadecyl Groups Detection :UV 254 nm (Naphthalene) 0 0 50 100 150 200 250 300 End-capping : Complete Hours in 50 mM TEA (pH 10) / CHHoOuHr=s7i0n/5300m, aMt 5T0E°CA (pH 10) / CH3OH = 70 / 30 , at 50°C AsAsshsohwonwinnitnhteheexepxepreimrimenetnat baobvoev,eI,nIneertrStSuustsataininCC1188mmaaiinnttaaiinneedd hhiigghh eeffffiicciieennccyyaannddppeeaakksshhaappeefoforr330000hhrsrs Carbon Loading : 14 % USP Code : L1 pH Range : 1-10 InertSustain C18 inherits the advantages of all the current Inertsil HPLC columns (e.g., extremely low operating back pressure, superior inertness to typically any analyte, high efficiency and compatibility with a wide range of solvents, consistent performance from column-to-column and lot-to-lot), with the additional feature of the extended pH range durability. InertSustain = Performance, Reliability, Reproducibility, Versatility Strong Basic Compound Test Strong Acidic Compound Test Strong Chelating Compound Test 1 2 1 Sharp peak shape and reproducible 3 chromatograms are obtained even with dextromethorphan which is a very strong basic compound. Lot.1 1 Lot.1 Lot.1 Lot.2 Lot.2 Lot.2 Lot.3 Lot.3 Lot.3 0 2 4 6 8 10 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 Time(min) Time(min) Time(min) Conditions Conditions Conditions Column Size : 5 µm, 250 × 4.6 mm I.D. Column Size : 5 µm, 150 × 4.6 mm I.D. Column Size : 5 µm, 150 × 4.6 mm I.D. Eluent : A) CH3CN Eluent : A) CH3CN B) 0.1 % H3PO4 Eluent : A) CH3CN B 0.1 % H3PO4 B) 25 mM phosphate buffer (pH 7.0) A / B = 25 / 75, v / v A / B = 40 / 60, v / v A / B = 40 / 60, v / v Flow Rate : 1.0 mL / min Flow Rate : 1.0 mL / min Flow Rate : 1.0 mL / min Col. Temp. : 40 °C Col. Temp. : 40 °C Col. Temp. : 40 °C Detection : UV 254 nm Detection : UV 254 nm Detection : UV 220 nm Sample : 1. Brilliant Blue FCF Sample : 1. Hinokitiol Sample : 1. Dextromethorphan 2. Phenol 3. Salicylic acid E-8 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Dimensions 2 μm InertSustain C18 3 μm 5 μm InertSustain® C18 HPLC/UHPLC Columns (800 bar) 3 μm HP Series (200 bar) (200 bar) ID x Length (in mm) Guard Column E (200 bar) - (500 bar) IN5020-14306 IN5020-14206 1.0 x 250 - IN5020-14305 IN5020-14205 Reusable holder with universal self adjusting 1.0 x 150 - - IN5020-14304 IN5020-14204 connections and cartridges containing the same 1.0 x 100 - - IN5020-14303 IN5020-14203 1.0 x 75 - - IN5020-14302 IN5020-14202 high performance packing material 1.0 x 50 - - IN5020-14301 IN5020-14201 1.0 x 30 - - IN5020-07416 IN5020-07316 UHPLC Guard Column (800 bar) 2.1 x 250 IN5020-14355 - IN5020-07415 IN5020-07315 2.1 x 150 - IN5020-14416 IN5020-07417 IN5020-07317 Dedicated Holder 2.1 x 125 IN5020-14354 IN5020-14415 IN5020-07414 IN5020-07314 2.1 x 100 IN5020-14353 - IN5020-07413 IN5020-07313 PEEK Guide Packing 2.1 x 75 IN5020-14352 IN5020-14414 IN5020-07412 IN5020-07312 2.1 x 50 IN5020-14351 IN5020-14413 IN5020-07411 IN5020-07311 Tubing 2.1 x 30 - IN5020-14412 IN5020-07426 IN5020-07326 3.0 x 250 IN5020-14365 IN5020-14411 IN5020-07425 IN5020-07325 Replacement Cartridges 3.0 x 150 - IN5020-14426 IN5020-07427 IN5020-07327 3.0 x 125 IN5020-14364 IN5020-14425 IN5020-07424 IN5020-07324 Simple to use cartridge system which includes 3.0 x 100 IN5020-14363 - IN5020-07423 IN5020-07323 a tubing installed to the holder offering 3.0 x 75 IN5020-14362 IN5020-14424 IN5020-07422 IN5020-07322 minimized dead volume. 3.0 x 50 IN5020-14361 IN5020-14423 IN5020-07421 IN5020-07321 3.0 x 30 - IN5020-14422 IN5020-07436 IN5020-07336 4.0 x 250 - IN5020-14421 IN5020-07435 IN5020-07335 4.0 x 150 - - IN5020-07437 IN5020-07337 4.0 x 125 - - IN5020-07434 IN5020-07334 4.0 x 100 - - IN5020-07433 IN5020-07333 4.0 x 75 - - IN5020-07432 IN5020-07332 4.0 x 50 - - IN5020-07431 IN5020-07331 4.0 x 30 - - IN5020-07446 IN5020-07346 4.6 x 250 - - IN5020-07445 IN5020-07345 4.6 x 150 - IN5020-14446 IN5020-07447 IN5020-07348 4.6 x 125 - IN5020-14445 IN5020-07444 IN5020-07344 4.6 x 100 - - IN5020-07443 IN5020-07343 4.6 x 75 - IN5020-14444 IN5020-07442 IN5020-07342 4.6 x 50 - IN5020-14443 IN5020-07441 IN5020-07341 4.6 x 30 IN5020-14442 IN5020-14441 InertSustain C18 Guard Columns Dimensions 2 μm 2 μm 3 μm 3 μm HP 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 + 2 cartridges + 2 cartridges 2.1, 3.0 1.0 x 10 -- - - IN5020-19300 IN5020-19250 IN5020-19299 IN5020-19249 3.0 1.5 x 10 4.0, 4.6 2.1 x 10 IN5020-20365 IN5020-20314 IN5020-20357 IN5020-20306 IN5020-19400 IN5020-19350 IN5020-19399 IN5020-19349 2.1, 3.0 3.0 x 10 4.0, 4.6 4.0 x 10 IN5020-20382 IN5020-20331 IN5020-20374 IN5020-20323 - - - - 3.0 x 20 4.0 x 20 IN5020-20399 IN5020-20348 IN5020-20391 IN5020-20340 IN5020-19200 IN5020-19150 IN5020-19199 IN5020-19149 -- - - IN5020-19100 IN5020-19050 IN5020-19099 IN5020-19049 -- - - IN5020-19600 IN5020-19550 IN5020-19599 IN5020-19549 -- - - IN5020-19500 IN5020-19450 IN5020-19499 IN5020-19449 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-9 LIFE SCIENCE

Colonnes HPLC/UHPLC InertSustain® AQ-C18 Colonnes HPLC/UHPLC RReteennttioionnPProrpoepretiretsieosf IonfeIrtnSeursttSaiunsAtaQi-nC1A8Q- C 1 8 Retention Factor of acidic, basic and neutral compounds using 100% water mobile phasePhysical Properties I nertS ustain AQ-C18 R emarks : 1. T he horizontal and vertical retention factor Silica : ES Silica Gel Aquasil C18 Atlantis T3 values were calculated with reference to the Particle Size : 1.9 μm, 3 μm, 5 μm I nertsil ODS-4 I nertsil ODS-3 retention factor values obtained from I nertsil ODS-SP I nertSustain C18 I nertS ustain AQ-C18. Surface Area : 350 m2/g 2. T he horizontal values represents the retention factor of amylbenzene using Pore Size : 100 Å CAPCELL PAK C18-AQ methanol/water= 80/20. Synergi Fusion RP 3. T he vertical values represents the retention Pore Volume : 0.85 mL/g factor of acidic, basic and neutral compounds XSelect CSH C18 using 100% water mobile phase. Bonded Phase : Octadecyl Groups Retention Factor of amylbenzene using methanol/water= 80/20 End-capping : Complete Carbon Loading : 13% ® AQ-C18 InertSustainUSP Code : L1 pH Range : 1-10 InertSustain AQ-C18 columns are designed to achieve strong retention for highly polar compounds, which is a challenging goal in reversed-phase mode. The optimization of bonding of the C18 groups at equal distance to the silica gel enable InertSustain AQ-C18 to offer significant retention for highly polar compounds even under water-rich mobile phases. Analysis of Water-Soluble Vitamins Analysis of Organic Acids 3 PDA-210 nm 300 7 026 200 300 100 2 11 1 4 8 6 9 10 mAU mAU 200 2 4 5 37 8 10 1 5 9 11 12 6 100 0 20 0 6 8 0 5 10 15 024 Time (min) min Conditions: Conditions: Column: 5 μm, 150 × 4.6 mm I.D. Column : 3 μm, 150 × 4.6 mm I.D. Eluent: 50 mM NaH2PO4 in H2O Eluent: A: 0.1% H3PO4 in H2O B: CH3CN (pH 2.1, H3PO4) A/B = 99/1 (5 min)- 99/1 – (20min) – 80/20 (5min), 99/1 Flow Rate: 1.0 mL / min Flow Rate: 1.0 mL / min Col. Temp.: 40°C Col. Temp.: 40°C Detection: UV 210 nm Detection: UV 220 nm Inj. Vol. : 10 μL Inj. Vol.: 5μL Sample: Sample: 1. Oxalic acid 50 mg/L, 2. Tartaric acid 500 mg/L, 1. Pyridoxamine 2. Thiamin 3. Nicotinic Acid 4. Ascorbic acid 3. Formic acid 1000 mg/L, 4. Malic acid 1000 mg/L, 5. Lactic acid 1000 mg/L, 5. Nicotinamide 6. Pyridoxal 7. Pyridoxine 8. Pantonenic acid 6. Acetic acid 1000 mg/L, 7. Maleic acid 10 mg/L , 8. Citric acid 1000 mg/L, 9. Folic Acid 10. Cyanocobalamin 11. Riboflavin 12. Biotin 9. Pyroglutamic acid 100 mg/L, 10. Succinic acid 1000 mg/L, 11. Fumaric acid 10 mg/L E-10 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns InertSustain AQ-C18 InertSustain® AQ-C18 Dimensions 1.9 μm 3 μm HP Series 3 μm 5 μm Guard Column E (200 bar) HPLC/UHPLC Columns (800 bar) (500 bar) (200 bar) (200 bar) ID x Length (in mm) - - IN5020-89876 IN5020-89746 1.0 x 250 - IN5020-89745 1.0 x 150 - - IN5020-89875 IN5020-89744 1.0 x 100 - IN5020-89743 1.0 x 75 - - IN5020-89874 IN5020-89742 1.0 x 50 - IN5020-89741 1.0 x 30 - - IN5020-89873 IN5020-89707 2.1 x 250 IN5020-89940 IN5020-89706 2.1 x 150 - - IN5020-89872 IN5020-89705 2.1 x 125 IN5020-89939 IN5020-89704 2.1 x 100 - - IN5020-89871 IN5020-89703 2.1 x 75 IN5020-89938 IN5020-89702 2.1 x 50 - IN5020-89925 IN5020-89837 IN5020-89701 2.1 x 30 - IN5020-89715 3.0 x 250 IN5020-89943 IN5020-89924 IN5020-89836 IN5020-89714 3.0 x 150 - IN5020-89713 3.0 x 125 IN5020-89942 - IN5020-89835 IN5020-89712 3.0 x 100 - IN5020-89711 3.0 x 75 IN5020-89941 IN5020-89923 IN5020-89834 IN5020-89710 3.0 x 50 - IN5020-89709 3.0 x 30 - IN5020-89922 IN5020-89833 IN5020-89723 Reusable holder with universal self adjusting 4.0 x 250 - IN5020-89722 connections and cartridges containing the same 4.0 x 150 - IN5020-89921 IN5020-89832 IN5020-89721 4.0 x 125 - IN5020-89720 high performance packing material 4.0 x 100 - IN5020-89920 IN5020-89831 IN5020-89719 4.0 x 75 - IN5020-89718 4.0 x 50 - IN5020-89931 IN5020-89845 IN5020-89717 4.0 x 30 - IN5020-89731 4.6 x 250 - IN5020-89930 IN5020-89844 IN5020-89730 4.6 x 150 - IN5020-89729 4.6 x 125 - - IN5020-89843 IN5020-89728 4.6 x 100 - IN5020-89727 4.6 x 75 - IN5020-89929 IN5020-89842 IN5020-89726 4.6 x 50 - IN5020-89725 4.6 x 30 IN5020-89928 IN5020-89841 UHPLC Guard Column (800 bar) IN5020-89927 IN5020-89840 IN5020-89926 IN5020-89839 Dedicated Holder - IN5020-89853 PEEK Guide Packing - IN5020-89852 - IN5020-89851 - IN5020-89850 - IN5020-89849 Tubing - IN5020-89848 Replacement Cartridges - IN5020-89847 Simple to use cartridge system which includes a tubing installed to the holder offering IN5020-89937 IN5020-89861 minimized dead volume. IN5020-89936 IN5020-89860 - IN5020-89859 IN5020-89935 IN5020-89858 IN5020-89934 IN5020-89857 IN5020-89933 IN5020-89856 IN5020-89932 IN5020-89855 InertSustain AQ-C18 Guard Columns Dimensions 1.9 μm 1.9 μm 3 μm HP 3 μm HP 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 + 2 cartridges + 2 cartridges 2.1, 3.0 1.0 x 10 -- - - IN5020-89911 IN5020-89910 IN5020-89809 IN5020-89908 3.0 1.5 x 10 4.0, 4.6 2.1 x 10 IN5020-89947 IN5020-89944 IN5020-89827 IN5020-89824 IN5020-89913 IN5020-89912 IN5020-89811 IN5020-89810 2.1, 3.0 3.0 x 10 4.0, 4.6 4.0 x 10 IN5020-89948 IN5020-89945 IN5020-89828 IN5020-89825 - - - - 3.0 x 20 4.0 x 20 IN5020-89949 IN5020-89946 IN5020-89829 IN5020-89826 IN5020-89909 IN5020-89908 IN5020-89807 IN5020-89806 -- - - IN5020-89907 IN5020-89906 IN5020-89805 IN5020-89804 -- - - IN5020-89917 IN5020-89916 IN5020-89815 IN5020-89814 -- - - IN5020-89915 IN5020-89914 IN5020-89813 IN5020-89812 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-11 LIFE SCIENCE

Colonnes HPLC/UHPLC InertSustainSwift C18 HPLC Columns InertSustainSwift® C18 Rapid Elution of Samples in Isocratic Methods Colonnes HPLC/UHPLC The retentivity of InertSustainSwift C18 is weaker even comparing with a conventional C8 column, which makes it ideal for methods requiring maximum throughput and efficiency. Physical Properties 2 Conditions : A) CH3OH 3 Eluent B) H2O 4 A/B = 80/20, v/v 56 Column Size Flow Rate : 3 μm, 150 x 2.1 mm I.D. 1 Col. Temp. Detection : 0.3 mL/min InertSustainSwift C18 Sample : 40 ℃ : UV 254 nm Silica : ES Silica Gel 0 2 4 6 8 10 12 14 ：1. Uracil Time (min) Particle Size : 1.9 μm, 3 μm, 5 μm 2. Toluene InertSustain C8 3. Ethylbenzene 4. Propylbenzene 0 2 4 6 8 10 12 14 5. n-Butylbenzene Time (min) 6. n-Amylbenzene Surface Area : 200 m2/g Inertil ODS-SP 0 2 4 6 8 10 12 14 Time (min) Pore Size : 200 Å (20 nm) Inertil ODS-4 Pore Volume : 1.00 mL/g 0 2 4 6 8 10 12 14 Time (min) Bonded Phase : Octadecyl Groups InertSustain C18 End-capping : Complete 0 2 4 6 8 10 12 14 Carbon Loading : 9.0 % Time (min) Inertsil ODS-3 0 2 4 6 8 10 12 14 Time (min) Sample No.6, n-Amylbenzene was used to determine the hydrophobic property of columns. As shown above, InertSustainSwift C18 delivers rapid elution of samples in isocratic methods. USP Code : L1 pH Range : 1-10 InertSustainSwift C18 maintains the same extreme inertness, wide pH range and provides rapid separations with symmetric peaks. The optimization of surface area, pore size and chemical bonding delivers superior peak shapes. InertSustainSwift C18 is also ideal for LC/MS/MS applications which offer higher sensitivity results and enables MS compatible buffers to be used due to the extremely I inneerrtt suilsictaa igneSl.w ift C 1 8 H P L C C olum ns Rapid Analysis of Dissolution Test of Formulations InertSustainSwift C18 provides moderate retentivity of analytes without the accumulation of strong hydrophobic contaminants in the column. The optimized surface area (200 m2/g) and carbon load (9.0%) enables rapid elution of samples in isocratic methods which makes it ideal for formulation dissolution tests. 1 Conditions: . I nertSustainSwift C18 Column Size: 5 μm, 50 x 4.6 mm I.D. 1 Eluent: C=H530C0N//5H020O/1/C, Hv/3vC/OvOH Flow Rate: 1.0 mL/min Col. Temp.: 30 °C Inj.Vol.: 100 μL Detection: UV 225 nm Sample: 1. Valsartan The adsorption of peak leads to poor peak shape with longer analysis time. Other Brands C18 Column E-12 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns InertSustainSwift C18 InertSustainSwift® C18 Dimensions 1.9 μm 3 μm HP Series 3 μm 5 μm Guard Column E (200 bar) HPLC/UHPLC Columns (800 bar) (500 bar) (200 bar) (200 bar) ID x Length (in mm) - - IN5020-88165 IN5020-88043 1.0 x 250 - IN5020-88042 1.0 x 150 - - IN5020-88164 1.0 x 125 - IN5020-88041 1.0 x 100 - - IN5020-88040 1.0 x 75 - IN5020-88039 1.0 x 50 - - IN5020-88163 IN5020-88038 1.0 x 30 - IN5020-88006 2.1 x 250 IN5020-88231 - IN5020-88162 IN5020-88005 2.1 x 150 - IN5020-88249 2.1 x 125 IN5020-88230 - IN5020-88161 IN5020-88004 2.1 x 100 - IN5020-88003 2.1 x 75 IN5020-88228 - IN5020-88160 IN5020-88002 2.1 x 50 - IN5020-88001 2.1 x 30 - IN5020-88214 IN5020-88129 IN5020-88013 3.0 x 250 IN5020-88236 IN5020-88012 3.0 x 150 - IN5020-88213 IN5020-88128 IN5020-88250 3.0 x 125 IN5020-88235 IN5020-88011 3.0 x 100 - - IN5020-88253 IN5020-88010 3.0 x 75 IN5020-88233 IN5020-88009 3.0 x 50 - IN5020-88212 IN5020-88127 IN5020-88008 Reusable holder with universal self adjusting 3.0 x 30 - IN5020-88020 connections and cartridges containing the same 4.0 x 250 - - IN5020-88126 IN5020-88019 4.0 x 150 - IN5020-88251 high performance packing material 4.0 x 125 - IN5020-88210 IN5020-88125 IN5020-88018 4.0 x 100 - IN5020-88017 4.0 x 75 - - IN5020-88124 IN5020-88016 4.0 x 50 - IN5020-88015 4.0 x 30 - IN5020-88220 IN5020-88136 IN5020-88027 4.6 x 250 - IN5020-88026 4.6 x 150 - IN5020-88219 IN5020-88135 IN5020-88252 4.6 x 125 - IN5020-88025 4.6 x 100 - - IN5020-88254 IN5020-88024 4.6 x 75 - IN5020-88023 4.6 x 50 - IN5020-88218 IN5020-88134 IN5020-88022 UHPLC Guard Column (800 bar) 4.6 x 30 - IN5020-88133 IN5020-88216 IN5020-88132 Dedicated Holder - IN5020-88131 PEEK Guide Packing - IN5020-88143 - IN5020-88142 - IN5020-88255 - IN5020-88141 Tubing - IN5020-88140 Replacement Cartridges - IN5020-88139 Simple to use cartridge system which includes a tubing installed to the holder offering - IN5020-88138 minimized dead volume. IN5020-88226 IN5020-88150 IN5020-88225 IN5020-88149 - IN5020-88256 IN5020-88224 IN5020-88148 - IN5020-88147 IN5020-88222 IN5020-88146 - IN5020-88145 InertSustainSwift C18 Guard Columns Dimensions 1.9 μm 1.9 μm 3 μm HP 3 μm HP 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 + 2 cartridges + 2 cartridges 2.1, 3.0 1.0 x 10 -- - - IN5020-88200 IN5020-88199 IN5020-88106 IN5020-88105 3.0 1.5 x 10 4.0, 4.6 2.1 x 10 IN5020-88244 IN5020-88238 IN5020-88243 IN5020-88237 IN5020-88202 IN5020-88201 IN5020-88108 IN5020-88107 2.1, 3.0 3.0 x 10 4.0, 4.6 4.0 x 10 IN5020-88246 IN5020-88240 IN5020-88245 IN5020-88239 - - - - 3.0 x 20 4.0 x 20 IN5020-88248 IN5020-88242 IN5020-88247 IN5020-88241 IN5020-88198 IN5020-88197 IN5020-88104 IN5020-88103 -- - - IN5020-88196 IN5020-88195 IN5020-88102 IN5020-88101 -- - - IN5020-88206 IN5020-88205 IN5020-88112 IN5020-88111 -- - - IN5020-88204 IN5020-88203 IN5020-88110 IN5020-88109 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-13 LIFE SCIENCE

Colonnes HPLC/UHPLC InertSustain® C8 Colonnes HPLC/UHPLC First Choice Column C18 Physical Properties Change in Change in Retentivity Selectivity Silica : ES Silica Gel Particle Size : 2 μm, 3 μm, 5 μm C8 Phenyl Surface Area : 350 m2/g Change in Selectivity Pore Size : 100 Å (10 nm) Pore Volume : 0.85 mL/g Bonded Phase : Octyl Groups End-capping : Complete Carbon Loading : 8% USP Code : L7 pH Range : 1-10 InertSustain C8 is an octyl group (C8) bonded column delivering the same extreme inertness to any type of compounds just like InertSustain C18, which enables rapid analysis of highly hydrophobic compounds delivering symmetric peaks over a wide pH range. InertSustain C18 InertSustain C8 2 2 3 4 9.39 min 3 15.43 min 5 4 6 5 6 1 1 0 10 20 0 10 20 Time(min) Time(min) Analysis of Losartan Potassium and Hydrochlorothiazide Tablets, Assay Test (Based on the Condition of United States Pharmacopeia 36-NF31) Conditions: 2 Column: InertSustain C8 (5 μm, 150 × 4.0 mm I.D.) CCHHECC3l3oouCClenuNNnmdt/intBiounfsfe::rIA*n)eC=rHt7S3Cu/Ns9t/a3Biun, ffCve8/r(v5*(μ=Pm7r/,e91m35,0vi/×xv)4(P.0remmmix)I.D.) 1 Eluent: A) B) A/B = 100/0 – 12Bm)CHin3C–N 92/8 – 16min – 38/62 – 2min – 100/0 –A– /25Bmm=i1ni0n–0/1–00–01/10020–m/5i0nm,–ivn9/–2v/180–0/106,vm/vin – 38/62 Flow Rate: 1.0 mFlLo/wmRainte : 1.0mL/min Col. Temp.: 35 °CCDoelt.eTcetmiopn. : 35 °C : UV280 nm(GL-7450 UV Detector) DInejetecctitoionnV:o Ul:V2208ISn0aμjmeLncptmileon(VGolL. -7:: 12240..5LHμo0yLsdaUrrotVcahnDloPreootttaehsicsaituzoimdre)00.4.1mmgg//mmLL Sample: 1. HydrochlorothTiaailzinigdfeac0to.1r omf 2g:/1m.79L( < 2.5) 2. Lo*saBrutffaenr :P1.o2t5ags/sLioufmmo0no.4bamsicgp/omtaLssium phosphate and Tailing1.f5agc/tLoorf doibfa2sic:s1o.d7iu9m(p<ho2sp.5ha)te n water. 0 10 20 30 * Buffer: 1.25 g/L of monobasic potassium phosphate and 1.5 g/L of dibasic sodium phosphate in water. Time(min) E-14 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Dimensions 2 μm InertSustain C8 3 μm 5 μm InertSustain® C8 HPLC/UHPLC Columns (800 bar) 3 μm HP Series (200 bar) (200 bar) ID x Length (in mm) Guard Column E (200 bar) - (500 bar) IN5020-16173 IN5020-16044 1.0 x 250 - IN5020-16172 IN5020-16043 Reusable holder with universal self adjusting 1.0 x 150 - - IN5020-16171 IN5020-16042 connections and cartridges containing the same 1.0 x 100 - - IN5020-16170 IN5020-16041 1.0 x 75 - - IN5020-16169 IN5020-16040 high performance packing material 1.0 x 50 - - IN5020-16168 IN5020-16039 1.0 x 30 - - IN5020-16137 IN5020-16007 UHPLC Guard Column (800 bar) 2.1 x 250 IN5020-16239 - IN5020-16136 IN5020-16006 2.1 x 150 - IN5020-16222 IN5020-16855 IN5020-16851 Dedicated Holder 2.1 x 125 IN5020-16238 IN5020-16221 IN5020-16135 IN5020-16005 2.1 x 100 IN5020-16237 - IN5020-16134 IN5020-16004 PEEK Guide Packing 2.1 x 75 IN5020-16236 IN5020-16220 IN5020-16133 IN5020-16003 2.1 x 50 IN5020-16235 IN5020-16219 IN5020-16132 IN5020-16002 Tubing 2.1 x 30 - IN5020-16218 IN5020-16144 IN5020-16014 3.0 x 250 IN5020-16244 IN5020-16217 IN5020-16143 IN5020-16013 Replacement Cartridges 3.0 x 150 - IN5020-16228 IN5020-16856 IN5020-16852 3.0 x 125 IN5020-16243 IN5020-16227 IN5020-16142 IN5020-16012 Simple to use cartridge system which includes 3.0 x 100 IN5020-16242 - IN5020-16141 IN5020-16011 a tubing installed to the holder offering 3.0 x 75 IN5020-16241 IN5020-16226 IN5020-16140 IN5020-16010 minimized dead volume. 3.0 x 50 IN5020-16240 IN5020-16225 IN5020-16139 IN5020-16009 3.0 x 30 - IN5020-16224 IN5020-16151 IN5020-16021 4.0 x 250 - IN5020-16223 IN5020-16150 IN5020-16020 4.0 x 150 - - IN5020-16857 IN5020-16853 4.0 x 125 - - IN5020-16149 IN5020-16019 4.0 x 100 - - IN5020-16148 IN5020-16018 4.0 x 75 - - IN5020-16147 IN5020-16017 4.0 x 50 - - IN5020-16146 IN5020-16016 4.0 x 30 - - IN5020-16158 IN5020-16028 4.6 x 250 - - IN5020-16157 IN5020-16027 4.6 x 150 - IN5020-16234 IN5020-16858 IN5020-16854 4.6 x 125 - IN5020-16233 IN5020-16156 IN5020-16026 4.6 x 100 - - IN5020-16155 IN5020-16025 4.6 x 75 - IN5020-16232 IN5020-16154 IN5020-16024 4.6 x 50 - IN5020-16231 IN5020-16153 IN5020-16023 4.6 x 30 IN5020-16230 IN5020-16229 InertSustain C8 Guard Columns Dimensions 2 μm 2 μm 3 μm 3 μm HP 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 + 2 cartridges + 2 cartridges 2.1, 3.0 1.0 x 10 -- - - IN5020-16208 IN5020-16207 IN5020-16107 IN5020-16106 3.0 1.5 x 10 4.0, 4.6 2.1 x 10 IN5020-20366 IN5020-20315 IN5020-20358 IN5020-20307 IN5020-16210 IN5020-16209 IN5020-16109 IN5020-16108 2.1, 3.0 3.0 x 10 4.0, 4.6 4.0 x 10 IN5020-20383 IN5020-20332 IN5020-20375 IN5020-20324 - - - - 3.0 x 20 4.0 x 20 IN5020-20400 IN5020-20349 IN5020-20392 IN5020-20341 IN5020-16206 IN5020-16205 IN5020-16105 IN5020-16104 -- - - IN5020-16204 IN5020-16203 IN5020-16103 IN5020-16102 -- - - IN5020-16214 IN5020-16213 IN5020-16113 IN5020-16112 -- - - IN5020-16212 IN5020-16211 IN5020-16111 IN5020-16110 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-15 LIFE SCIENCE

Colonnes HPLC/UHPLC Colonnes HPLC/UHPLC InertSustainSwift® C8 InertSustainSwift C8 InertSustainSwift C8 Phys●icBalaPseroMpaetretriieasl : High Purity ES Silica Gel Silica● P article Size: High :P1u.9ritμymE,S3Sμilmic,a5Gμeml Parti●clSeuSrifzaec e Area: 1.9 μ:m2,030 mμm2/g, 5 μm eexotrpetmimeizeindPSeuo2rtr0r●●●nfe0ae●●●SBPScÅsauaPFPeiszrprsuooetfAtoeinarro crreecMeleaeetSVaasinooSiAi tyzzenliereuzrateieymslaapiGlleie::cr22aoo00uef00np:::caoÅm2H1:::b.m0i921Ol2(ge0/2.p0chμ0sgm00ot0mtPyuoÅ2nmln,u/gam3dr(Ln2ist)μ/a0gymljyunEz,smSe5t )aSlμinkimledica Gel to severalPdoor●zee●PnVoEokrnDleudaSm-.cizeae pping: 1.00: 2:m0Y0Le/Åsg (20 nm) EBnod●●●n-d●●●FPEceauonUpCdpnrdHSaepcP-rPctiRVbhniaooCaagpnnlsno upeagdmLil neeoGegardo:: iuYOnepgcst:::yO1Yl:::.ec016Lst0%7-ym1l 0L/g ConditionsCar●bCoanrLbooandLinoga din: g6%: 6% SizeUp::HSA5BA●●P))/μRBHCmIIrpUC=ann2HeO1H3o1enSteC5a0r0drgNP/iRt9tn×See0SCa,4 uv pu.n/6ovsesgmtdptameaetiinidIn.SDeS.wswi::afit1LnftC7d-1C1o::088l1L,ig7wi-oshn1ai0ucnchleoisoctitdiydeleagsl Column roup (C8) bo nded column offering the same extreme inertness to any t ype of compounds just like Eluent for analyzing low polarity analytes. In addition, the optimized 200 Å pore size silica enables to analyze and which have a molecular weight from several kDa to several dozen kDa. Flow Rate : 14I.0n0C̊ emrL/tmSinustainSwift Col. Temp : C8 is an octyl group (C8) bonded column offering the same extreme inertness to any type of compounds just like Detection IInn:: eeU12rI..nVrreRCtteFt3ehSSa0rtoii0uuitnlgenSoncsslmauuptt(lVcaaseriitftiiaepeannmrtoiSSiinln1d(wwSVAei):twsiiaffmCittafitnnCCoCDd813m1)8o8isl,p,igwawaonhhnriiuioccschcholteisyinosltidiigodderefeaoslaRufwlopefhrot(iaceCrnh8naa)hntlyaibazvvolieyinntzgaydinlmeogwdollopceoowclulaulpramiotrylnwaaeroniigatffyhleytatrefinrnsoa.gmlIynttshaeeedsvd.esiaIrtnaimolnake,dDtedhaxeitttiooroepsnmtei,mvteeihzriaeenldeodr2pot0ztni0emenÅsiskzpDetoodare.a2sn0izy0etÅysiplpiceoaroeefnscaiozbmelesspioltiocuaanndeasnlyajzubesletasnlidktoe Sample an34a.. Pαly-htoyzlcelooqpauhiennroodnl e(V1r(iVetaitmtaaminiinnE)Kp1)eptides and oligonucleotides which have a molecular weight frCoonmditsioenvs eral kDa to several dozen kDa. Co5Fm. Iimpgpuaurirtriiesesoof1n1 o: fCRoemtepnatirviistyon of Retentivity Column Size : 5 μm 150 × 4.6 mm I.D. Eluent : A) H2O 12 5 B) CH3CN Conditions A/B = 10/90,v/v ColuFmlonwSRizaete : 5:μ1m.01m50L/×m4in.6 mm I.D. 3 ElueCnotl. Temp: A:) 4H02OC̊ Detection B:) UCVH33C0N0 nm 2 Sample A:/B1.=R1e0t/in9o0l,v(/Vvitamin A) 45 Flow Rate : 1.02m. CL/hmoilnecalciferol (Vitamin D3) 3 Col. Temp : 40 3C̊ . α-tocopherol (Vitamin E) Detection : UV43. 0P0hynlmloquinone (Vitamin K1) 0 10 20 30 40 50 Sample : 1. R5.eItminoplu(rVitiietasmoifn 1A) 4 Time(min) 2. Cholecalciferol (Vitamin D3) InertSustainSwift C8 3. α-tocopherol (Vitamin E) 4. Phylloquinone (Vitamin K1) 0 10 20 30 40 50 5. Impurities of 1 1 Time(min) InertSustainSwift C8 25 1 3 2 45 0 10 3 20 30 40 50 Time(min)4 40 50 Conditions InertSustain C8 Colum0 n Size : 5 μm 21500 × 4.6 mm I.D. 20 30 Eluent : A) CH3CN Time(min) B) 25 mM K2HPO4 (pH 7.0I,nKeHr2PtSOu4) stain C8 A/B = 60/40, v/v AnaFCllFooylw.isTgRiesamuteporfeA:: 214n.00t:°miChAL/imsnintaamlyisniessof Antihistamines Detection : 230 nm 5 2 Injection.Vol. : 5 μL 10 12 FSiagmpulere 2: :1. AnalysisChlorpheniramine of Antihistamines Conditions 2. Triprolidine 3. HHoydmroocxhy1zl2oinrceycliz3ine 23 Column Size : 5 μm 250 × 4.6 mm I.D. 4. Clemastine 5. 4 Eluent : A) CH3CN B) 25 mM K2HPO4 (pH 7.0, KH2PO4) 1 Conditions A/B = 60/40, v/v 23 13 5 4 CoFlulmown RSaiztee : 5:μ1m.0 2m5L0/m×i4n.6 mm I.D. 4 EluCeonlt. Temp : A:) 4C0H°3CCN 5 Detection B:) 22350mnMmK2HPO4 (pH 7.0, KH2PO4) Injection.Vol.A:/B5=μL60/40, v/v 1 5 4 FloSwaRmaptlee : 1:.01m. CL/hmloinrpheniramine 02 4 6 8 10 12 02 46 8 10 12 Col. Temp : 40 2°C. Triprolidine Time (min) 02 Time (min) 5 Detection : 2303.nHmomochlorcyclizine InertSustainSwift C8 Hypersil BDS C8 Injection.Vol. : 5 μ4L. Hydroxyzine Sample : 1. C5.hClolrepmheansitrinaemine 02 4 6 8 10 12 4 68 10 12 2. Triprolidine E-16 Time (min) H1y8pe8Tirm8se-il2(mB2inD)6S-2C7875 3. Homochlorcyclizine :: [email protected]. Hyd:r:oxyziwneww.lifescience.ca CANADIAN 5. Clemastine InerLtSIFusEtaSinCSwIEifNt CC8E

InertSustainSwift C8 HPLC/UHPLC Columns ● Base Material : HIingehrtPSuursittayinESSwSifitliCc8a Gel InertSustainSwift® C8 IDDximLenegnths(ii●●nomnSPmsau) rrtfiacclee(SA810ir.z9e0eaμbmar) : 21.0903μmμm(m25,/g03H0μPbmSae,rr)5ieμsm 3 μm 5 μm Guard Column E (200 bar) HPLC/UHPLC Columns : (200 bar) (200 bar) 1.0 x ●●2PP50oorree SVoizleume- : 21.0000Åm(L2/0g-nm) IN5020-88471 IN5020-88347 : IN5020-88470 IN5020-88346 1.0 x ●1F50unctional G- roup : Octyl - IN5020-88345 IN5020-88344 1.0 x ●1E00nd-capping- : Yes - IN5020-88469 IN5020-88343 IN5020-88342 1.0 x ● 7C5arbon Load- ing : 6% - IN5020-88468 ● 5Up0HSPRCanogdee : L7 - IN5020-88467 1.0 x ● - : 1 - 10 - IN5020-88466 1.0 x 30 - 2.1 x In2e50rtSustainSw- ift C8 is an IoNc50t2y0l-8g8r5o18up (C8) bINo5n02d0e-8d843c2olumn ofINfe50ri2n0-g88t3h08e same extreme inertness to any type of compounds just like 2.1 x In1e5r0tSustaIiNn5S0w20i-f8t8C53158, which IiNs5i0d2e0-a88l 5f1o7r analyzinINg50lo2w0-8p84o3l1arity analyINt5e0s2.0-I8n83a0d7dition, the optimized 200 Å pore size silica enables to analyze and 2.1 x re1t2a5in peptides-and oligonucleotid-es which haveINa50m20o-8l8e4c3u0lar weighINt 5f0ro20m-88s3e06veral kDa to several dozen kDa. 2.1 x 100 IN5020-88534 IN5020-88516 IN5020-88429 IN5020-88305 2.1 x Fi7g5 ure 1 : C-omparison of -RetentivityIN5020-88428 IN5020-88304 IN5020-88303 Reusable holder with universal self adjusting IN5020-88302 2.1 x 50 1 IN5020-88533 IN5020-88515 IN5020-88427 IN5020-88316 conCnonedcittioionsns and cartridges containing the same IN5020-88315 2.1 x 30 - - IN5020-88426 IN5020-88314 Columhn iSgizhe :p5eμrmfo15r0m×a4.n6 cmem pI.Da. cking material IN5020-88313 Eluent : A) H2O IN5020-88312 3.0 x 250 2- IN5020-88522 IN5020-88440 IN505200-88311 B) CH3CN 3.0 x 150 IN5020-88521 IN5020-88439 IN5020-88310 3.0 x 125 IN5020-88538 5 IN5020-88438 IN5020-88324 A/B = 10/90,v/v 3.0 x 100 -3 - IN5020-88437 IN5020-88323 IN5020-88520 Flow Rate : 1.0 mL/min Col. Temp : 40 ̊C Detection : UV 300 nm IN5020-885374 Sample : 1. Retinol (Vitamin A) 2. Cholecalciferol (Vitamin D3) 3.0 x 75 - - IN5020-88436 3. α-tocopherol (Vitamin E) 3.0 x 50 0 IN5020-8180536 IN205020T-i8m8e5(m19in) 30 IN5020-8844305 UHPLC Guard54..CIPmohpyluullormiqtieunsinoo(8fne010(Vbitaamri)n K1) 3.0 x 30 - InertSust-ainSwift C8 IN5020-88434 Dedicated Holder 4.0 x 250 - - IN5020-88448 PEEK Guide Packing 4.0 x 150 1 - IN5020-88447 - 4.0 x 125 -2 - IN5 5020-88446 IN5020-88322 4.0 x 100 - 3- IN5020-88445 IN5020-88321 Tubing 4.0 x 75 - - 4 IN5020-88444 IN5020-88320 4.0 x 50 - - IN5020-88443 IN5020-88319 4.0 x 30 0 - 10 IN5020-8844402 IN505020-88318 20 - 30 - IN5020-88456 IN5020-88332 Time(min) Replacement Cartridges - IN5020-88455 IN5020-88331 4.6 x 250 IINn5e0r2t0S-8u8s5t2a6in C8 IN5020-88330 Simple to use cartridge system which includes IN5020-88329 a tubing installed to the holder offering 4.6 x 150 IN5020-88525 IN5020-88328 minimized dead volume. IN5020-88327 4.6 x 125 - - IN5020-88454 4.6 x F1i0g0ure 2 : A-nalysis of IAN5n02t0i-h88i5s24tamineIsN5020-88453 4.6 x 75 - - IN5020-88452 4.6 x 50 2 IN5020-88523 IN5020-88451 - 4.6 x 30 - - IN5020-88450 IN5020-88326 Conditions 13 23 Column Size : 5 μm 250 × 4.6 mm I.D. 4 Eluent : A) CH3CN InertSustainSwift C8 Guard Columns B) 25 mM K2HPO4 (pH 7.0, KH2PO4) 1 3 μm 4 A/B = 60/40, v/v Dimensions 1.9 μm 1.9 5μm 3 μm HP 3 μm HP (Guard Column E Set) 3 μm μm5(DCIFGn*luoeojIenaltw+.eccrTctdl2RiuoteiCdcaonmaoetn.reVlspturo1midlh.ngo::::eElsd5241Se3.0eμ0r0t°L)mCnLm/mRineCpalr5atcrμiedmmgeesnt Column Replacement Replacement *Includes 1 holder ReCpalr5atcriedmgeesnt ID (mm) ID x Length (mm) (UHPLC Guard Set) (UHPLC Guard Set) + 2 cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges + 2 cartridges + 2 cartridges Sample : 1. Chlorpheniramine 1.0 x 100 2- 4 6 -8 10 -12 0 -2 4 IN50260-885068 IN105020-8851205 IN5020-884120. TriproliIdNin5e020-88409 1.0 1.5 x 10 IN5020-88530 IN5020-88507 2.1 x IN5020-88542 TimeI(Nm5in0)20-88539 IN5020-88527 TIiNm5e0(2m0in-)88508 3. Homochlorcyclizine 1.5, 2.1 10 IN502I0n-8e8r5t4S3ustaiInNS50w20if-t88C5480 IN5020-88531 IN5020-88528 Hypersil B-DS C8 - IN5020-884142. HydroxIyNz5in0e20-88411 - 5. Clemastine - 2.1, 3.0 3.0 x 10 IN5020-88544 IN5020-88541 IN5020-88532 IN5020-88529 IN5020-88504 IN5020-88503 IN5020-88408 IN5020-88407 3.0 4.0 x 10 - - - - IN5020-88502 IN5020-88501 IN5020-88406 IN5020-88405 4.0, 4.6 3.0 x 20 - - - - IN5020-88512 IN5020-88511 IN5020-88416 IN5020-88415 2.1, 3.0 4.0 x 20 - - - - IN5020-88510 IN5020-88509 IN5020-88414 IN5020-88413 4.0, 4.6 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-17 LIFE SCIENCE

Colonnes HPLC/UHPLC InertSustain PFP InertSustain PFP Colonnes HPLC/UHPLC PIhnyseicarl tPrSopuertsiestain PFP ● B: aHsieghMaPtuerriiatly ES :SHiliigchaPGuerilty ES Silica Gel Silica ● PS: au3rrtfμiacmclee,SA5irzeμeam : 3 μm, 5 μm Particle Size ● : 350 m2/g Surface Area ● P: o3r5e0Simze2/g : 100 Å (10 nm) ●●PoPBraaersteSiciMlzeeaS teizrieal ● ::PF:H3uo1inrμ0gecmh0tVi,PooÅ5nuluμar(mi1lmtGy0erEnoSumpS)i::liP0c.ae8n5GtmaeflLlu/gorophenyl ● ●PoSruerfVacoeluAmreea ● :E:3n05d.08-cm5a2pm/pgLin/gg : Yes ●●BoPPnoodrreeeSVdoizPleuhmaese ● ::UC:10SaP0.r8Pe0b5noCÅmtnoaL(d1Lf/0eglouanodmrin)ogph::eL1n04y%3l ● ●EnFdu-nccatipopnainl gG roup● :p:PHCeoRnmtaanfpgluleeotroepheny:l2 - 7.5 ●●●●CUpHaSpECUrPHnSabRrdPCRoab-coCoannannodgLpgdeeLopee o ianadgdiinngg Ispnua::::e:::cttrYL21he1L2t0e4Sr-4a-0%ns3u77s3s%s..5πtw5a-iiπtnh, PFP columns are bonded with pentafluorophenyl groups to our newly developed ES silica gel, which delivers unique separation excellent peak shape and sensitivity. The chromatographic difference stems from the fact that the PFP phases provide interactions dipole, hydrogen bonding, and ionic interactions unlike conventional PFP columns. IInneertrStSusutastinaiPnFPPFcPoFlucimognluusrmearne1sb:oaCnrdeoemdbowpniathdriepsedonnwtafioltuhforspoepelhneectnatyiflvlguirtooyurpobspethtoweoneuyrelnngewrreolyvudepersvseteloodpoepudhrEanSsesewiliccloaylgdueeml,vwnehlsoicpheddeliEveSrssiulinciaqugeesle,pwarhaitciohn delivers unique psspuaehctptaheasranresassπtwip-oiπtrhn,oedvpxipicadoetelletlee,innhrtyntpdeserroawagkceisttnhIhinoabepeonrenxstdcSasiennuudlgslc,esthaaennintandsspiPtioieπvFniat-Piycπk.i,Tnsdhtheeirapacpcohterli1ooeman,snahtudyondgslirrkeaoepnghcseioictnnidvviebfifnteoytrin.eonnTdcahielnePsgFtc,ePhmarcnsoodflmruoimmoannttsohi.cegαrifana(ctpttreahtrhniaasc/tccdttihsiioe)ff=nPe1sFr.Pe3u7npnchleiaksesetscepomECrnoluosvevnniddetfiretniooitnnmistoe:nrtAah)acCletHioP3OfnFaHsPcBt)cHto2hOluatmtnhse. PFP A/B = 80/20, v/v Figure 1 : Comparison of selectivity between 2reversed phase columns Conditions Column Size : 5 μm,150 × 4.6 mm I.D. Flow Rate : 1.0 mL/min 0 2 468 1 0 Eluent: 1 2 A) CHDC3eoOtl.eTHcetimoBnp). H2::O4U0V°2C10 nm 1 α (tranCFsCECFlo/llooocuollwewuiunsnmdRm)tnRiat=ntiSaeoi t1znSee.si0:ze ::: AA15:.)/0μ BCm1A5m=H.,/1Lμ038B5O/m0m0mH/=i2×n,*Sc0Lαi48aB,1s/.(mv-)06t5mS/rHpva/m0tlin22ileOmbns×0e/cIn,.i4seDv)..a:6/nTv:dhm12et..rcatmrrainastsn-ioSI-sS.-toDiStlfiblt.rbeielebntneeenenteion factor of InertSustain PFP Time (miαn) (trans/cis) = 1.37 InertSustain Phenyl 1+2 2 0 2 4 6 8 1 0 1 2 CCDoeoltl.e.TcTetiemomnp. p.::: 4U0V°42C010°nCm Time (min) 1 0 DSeamtepcletion:1 :212.. ctrUiasn-VSs-t2iSlb1teil0bneennem (trans S/*ccaαisims(-tSr)apti=nlblsee1/c:n.ise0 ) a3: nTdhetrar12ant..sio-ctSroitsafilr-bneSetsnet-ineSltbitoienlbnfaeectnoreof 02 4 Time 6 8 InertSustain Phenyl 1+2 (min)α (trans/cis) = 1.0 InertSustain C18 α 1 2*α (trans/cis): The ratio of retention factor of 0 2 4 6 8 1 0 1 2 cis-Stilbene and 1t.racins-sS-tSilbtielnbeene 2. trans-Stilbene Time (min) ( cis) ( t r a n s) InertSustain C18 0 4 Time 6 (trans/cis)8 10 12 (miαn) = 1.03 1 2 0 Figure 2: Comparis4on of high6 polarity c8 ompound10s analysis12 1. cis-Stilbene 2 . trans-Stilbene Time (min) ( cis) ( t r a n s) InertSustain PFP 1 Middle Compound Basic Compound Compound 4 23 Figure 2: Comparison of high polarity compounds analysis InertSustain PFP 1 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 Time (min) Middle Compound Basic Compound Compound 1 : Uracil 2 : Cytosine 44 23 1 2 Luna 1P.0FP 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 3 Time (min) 7.0 8.0 2 3.0 4 4 1 : Uracil 3 : Th2ym: Cinyetosine 4 : Adenine 1 1.0 1 2.0 4.0 5.0 6.0 9.0 10.0 Time (min) Luna PFP Hypersil GOLD 2 3 Conditions PFP 3 Hypersil GOLD 3 : Thymine Column 4:: 03:.1μAm%d, e1H5n0Cin×OeO2.1H mm I.D. PFP 1.0 2.0 3.0 7.0 8.0 Eluent in H2O 4 4.0 5.0 6.0 9.0 10.0 Flow Rate : 0.2 mL/min 1 Time (min) 3.0 Col. Temp. : 40 °C 1.0 2.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 Detection : UV 254 nm Time (min) 2 Conditions 3 Column : 3 μm, 150 × 2.1 mm I.D. Eluent : 0.1 % HCOOH in H2O Flow Rate : 0.2 mL/min Col. Temp. : 40 °C 038 HPL1.C0 Colu2m.0 n Ca3ta.0log 4.0 5.0 6.0 7.0 8.0 9.0 Detection : UV 254 nm Time (min) 10.0 E-18 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns InertSustain PFP InertSustain PFP InertSustain® PFP Dimensions 3 μm HP Series 3 μm 5 μm● Base Material : High Purity ES Silica Gel HPLC/UHPLC Columns (500 bar) (200 bar) (200 bar)● Particle Size ID x Length (in mm) ● Surface Area - : 3 μm, 5 μm 1.0 x 250 - : 350 m2/g 1.0 x 150 - 1.0 x 125 - IN5020-87873●● Pore Size IN50:: 2010.080-58Åm7L(71/04g6nm) 1.0 x 100 Pore Volume 1.0 x 75 1.0 x 50 ● Functional Group : Pentafluorophenyl 1.0 x 30 2.1 x 250 IN5020-87872● End-capping IN50: 2Ye0s-87745 2.1 x 150 2.1 x 125 ● Carbon Loading : 10% 2.1 x 100 - ● USP Code : L4-3 2.1 x 75 2.1 x 50 ● pH Range : 2 - 7.5 2.1 x 30 3.0 x 250 IN5020-87871 IN5020-87744 3.0 x 150 3.0 x 125 - IN5020-87870InertSustain PFP IcNo5lu0m2n0s-a8r7e7b4o3nded with pentafluorophenyl groups to our newly developed ES silica gel, which delivers unique separation 3.0 x 100 Guard Column E (200 bar)patterns with excellent peak shape and sensitivity. The chromatographic difference stems from the fact that the PFP phases provide interactions 3.0 x 75 3.0 x 50 - IN5020-87869such as π-π, dipoIleN, 5hy0d2r0og-8en77bo4n2ding, and ionic interactions unlike conventional PFP columns. 3.0 x 30 4.0 x 250 - IN5020-87868Figure 1 : ComINp5a0r2is0o-8n77o4f1selectivity between reversed phase columns 4.0 x 150 4.0 x 125 IN5020-87922 IN5020-87834 InertSuIsNta5i0n2P0F-8P7707 1 α (trans/cis) = 1.37 Conditions 4.0 x 100 IN5020-87921 IN5020-87833 4.0 x 75 IN5020-87832 Eluent : A) CH3OH B) H2O 4.0 x 50 - IN5020-87831 4.0 x 30 IN5020-87920 IN5020-87830 A/B = 80/20, v/v 4.6 x 250 IN5020-87919 IN5020-87829 4.6 x 150 IN5020-87918 IN5020-87828 IN5020-87706 2 Column Size : 5 μm,150 × 4.6 mm I.D. 4.6 x 125 IN5020-87917 IN5020-87842 4.6 x 100 IN5020-87928 IN5020-87841 Flow Rate : 1.0 mL/min 4.6 x 75 IN5020-87927 4.6 x 50 Col. Temp. : 40 °C 4.6 x 30 IN0 5020-877205 4 6 8 1 0 1 2 Detection : UV 210 nm InertSIuNs5t0ai2n0P-8h7e7n0y4l 1+2 Time (min) : 1. cis-Stilbene Sample 2. trans-Stilbene α (trans/cis) = 1.0 *α (trans/cis) : The ratio of retention factor of cis-Stilbene and trans-Stilbene IN5020-87703 4 6 R8 eusab1l0e holde12r with universal self adjusting Time (min) 02 connecαt(itroa1nns/csis)a=n1.0d3 cartridges containing the same 6 InertSIuNs5t0ai2n0C-8178702 Time (min) high performance packing material2 IN5020-87701 IN5020-87715 1. cis-Stilbene 2 . trans-Stilbene 0 ( cis) ( t r a n s) IN5020-87714 4 8 10 12 - IN5020-87840 IN5020-87713 IN5020-87926 Figure 2: Comparison of high polarity compounds analysis IN5020-87925 IN5020-87924 IN5020-87839 IN5020-87712 IN5020-87923 InertSustain PFP 1 Middle Compound Basic Compound - 4 Compound - IN5020-87838 IN5020-87711 23 - IN5020-87837 UHPLC Guard Column (800 bar) - IN5020-87710 - - IN5020-87836 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 - IN5020-87850 Time (min) 3 IN5020-87849 Luna PFP IN5020-87709 1 : Uracil 2 : Cytosine 4 IN5020-87723 1 Dedicated Holder PEEK Guide Packing 2 IN5020-87722 IN5020-87848 IN5020-877211.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 3 : Thymine 4 : Adenine IN5020-87847 Time (min) IN5020-87720 4 1 IN5020-87846 Hypersil GOLDIN5020-877192 3 Conditions PFP Column : 3 μm, 150 × 2.1 mm I.D. Tubing Eluent : 0.1 % HCOOH in H2O IN5020-87845 IN5020-87718 Flow Rate : 0.2 mL/min Col. Temp. : 40 °C 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 Detection : UV 254 nm IN5020-87844 IN5020-87717 Time (min) IN5020-87934 IN5020-87858 IN5020-87731 Replacement Cartridges IN5020-87933 IN5020-87805378 HPLC Column ICNat5al0og20-87730 - IN5020-87856 IN5020-87729 Simple to use cartridge system which includes IN5020-87932 IN5020-87855 IN5020-87728 a tubing installed to the holder offering IN5020-87931 IN5020-87854 IN5020-87727 minimized dead volume. IN5020-87930 IN5020-87853 IN5020-87726 IN5020-87929 IN5020-87852 IN5020-87725 InertSustain PFP Guard Columns Dimensions 3 μm HP 3 μm HP 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 + 2 cartridges 2.1, 3.0 1.0 x 10 - - IN5020-87908 IN5020-87907 IN5020-87808 IN5020-87807 3.0 1.5 x 10 4.0, 4.6 2.1 x 10 IN5020-87824 IN5020-87821 IN5020-87910 IN5020-87909 IN5020-87810 IN5020-87809 2.1, 3.0 3.0 x 10 4.0, 4.6 4.0 x 10 IN5020-87825 IN5020-87822 - - - - 3.0 x 20 4.0 x 20 IN5020-87826 IN5020-87823 IN5020-87906 IN5020-87905 IN5020-87806 IN5020-87805 - - IN5020-87904 IN5020-87903 IN5020-87804 IN5020-87803 - - IN5020-87914 IN5020-87913 IN5020-87814 IN5020-87813 - - IN5020-87912 IN5020-87911 IN5020-87812 IN5020-87811 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-19 LIFE SCIENCE

Colonnes HPLC/UHPLC InertSustain® Phenyl Colonnes HPLC/UHPLC First Choice Column C18 Physical Properties Change in Change in Retentivity Selectivity Silica : ES Silica Gel Particle Size : 2 μm, 3 μm, 5 μm Surface Area : 350 m2/g Pore Size : 100 Å (10 nm) C8 Phenyl Pore Volume : 0.85 mL/g Change in Selectivity Bonded Phase : Phenyl Groups End-capping : None Carbon Loading : 10 % USP Code : L11 pH Range : 2-7.5 InertSustain Phenyl delivers extremely unique reverse phase characteristics that are critical to resolving compounds that cannot be separated on a C18 or C8 phase. InertSustain Phenyl provides not only π-π interactions, but also hydrogen bonding secondary interactions, which results in retaining polar compounds at the same time. As the phenyl groups are directly bonded to the silica gel, InertSustain Phenyl is compatible with the analysis of structural isomers due to its high stereo-selectivity while other alkyl phenyl type columns fail to separate. Comparison of Selectivity between InertSustain C18, Phenyl and other Phenyl brand columns InertSustain C18 InertSustain Phenyl Other Phenyl brand columns 4 2,4 4 13 1 1 2,3 2 3 0 20 40 60 80 0 10 20 30 0 10 20 T ime (min) T ime (min) T ime (min) Conditions Column Size ：5 μm, 150 x 4.6 mm I.D. 1. Butylbenzene 2. o-Terphenyl 3. Amylbenzene 4. Triphenylene Eluent ： A) CH3OH (Steric Structure) (Planar Structure) Flow Rate Col. Temp. B) H2O Detection A/B = 70/30, v/v ：0.8 mL / min ：40 ℃ ：UV 254 nm Analysis of Structural Isomers InertSustain Phenyl Other Phenyl brand Column A Other Phenyl brand Column B 1 2,3 2,3 23 Not Separated 1 1 Not Separated 0 10 20 30 40 0 10 20 30 40 0 10 20 30 40 T ime (min) T ime (min) T ime (min) Conditions OH Column Size ：5 μm, 150 x 4.6 mm I.D. OH OH Eluent C H3 Col. Temp. ：A) HCH2O3OH Flow Rate B) 1. o-Cresol Detection A/B = 20/80, v/v ：40 ℃ C H3 ：0.8 mL / min 2. m-Cresol ：UV 254 nm C H3 3. p-Cresol E-20 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Dimensions 2 μm InertSustain Phenyl 3 μm 5 μm InertSustain® Phenyl HPLC/UHPLC Columns (800 bar) 3 μm HP Series (200 bar) (200 bar) ID x Length (in mm) Guard Column E (200 bar) - (500 bar) IN5020-16344 - IN5020-16343 1.0 x 250 - - IN5020-16473 IN5020-16342 1.0 x 150 - IN5020-16341 1.0 x 100 - - IN5020-16472 IN5020-16340 1.0 x 75 - IN5020-16339 1.0 x 50 - - IN5020-16471 IN5020-16307 1.0 x 30 IN5020-16539 IN5020-16306 2.1 x 250 IN5020-16538 - IN5020-16470 IN5020-16305 2.1 x 150 IN5020-16537 IN5020-16304 2.1 x 100 IN5020-16536 - IN5020-16469 IN5020-16303 2.1 x 75 IN5020-16535 IN5020-16302 2.1 x 50 - - IN5020-16468 IN5020-16314 2.1 x 30 IN5020-16544 IN5020-16313 3.0 x 250 IN5020-16543 IN5020-16522 IN5020-16437 IN5020-16312 3.0 x 150 IN5020-16542 IN5020-16311 3.0 x 100 IN5020-16541 IN5020-16521 IN5020-16436 IN5020-16310 3.0 x 75 IN5020-16540 IN5020-16309 3.0 x 50 - IN5020-16520 IN5020-16435 IN5020-16321 3.0 x 30 - IN5020-16320 4.0 x 250 - IN5020-16519 IN5020-16434 4.0 x 150 - - 4.0 x 125 - IN5020-16518 IN5020-16433 IN5020-16319 Reusable holder with universal self adjusting 4.0 x 100 - IN5020-16318 connections and cartridges containing the same 4.0 x 75 - IN5020-16517 IN5020-16432 IN5020-16317 4.0 x 50 - IN5020-16316 high performance packing material 4.0 x 30 - IN5020-16528 IN5020-16444 IN5020-16328 4.6 x 250 - IN5020-16327 4.6 x 150 - IN5020-16527 IN5020-16443 IN5020-16326 - IN5020-16325 - IN5020-16526 IN5020-16442 IN5020-16324 IN5020-16323 IN5020-16525 IN5020-16441 IN5020-16524 IN5020-16440 IN5020-16523 IN5020-16439 UHPLC Guard Column (800 bar) - IN5020-16451 - IN5020-16450 Dedicated Holder -- PEEK Guide Packing - IN5020-16449 - IN5020-16448 - IN5020-16447 - IN5020-16446 Tubing IN5020-16534 IN5020-16458 Replacement Cartridges IN5020-16533 IN5020-16457 Simple to use cartridge system which includes a tubing installed to the holder offering 4.6 x 100 IN5020-16532 IN5020-16456 minimized dead volume. 4.6 x 75 IN5020-16531 IN5020-16455 4.6 x 50 4.6 x 30 IN5020-16530 IN5020-16454 IN5020-16529 IN5020-16453 InertSustain Phenyl Guard Columns Dimensions 2 μm 2 μm 3 μm HP 3 μm HP 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 1.0 x 10 + 2 cartridges + 2 cartridges 1.5, 2.1 1.5 x 10 + 2 cartridges + 2 cartridges 2.1, 3.0 2.1 x 10 3.0 x 10 -- - - IN5020-16508 IN5020-16507 IN5020-16407 IN5020-16406 3.0 4.0 x 10 4.0, 4.6 3.0 x 20 IN5020-20367 IN5020-20316 IN5020-20359 IN5020-20308 IN5020-16510 IN5020-16509 IN5020-16409 IN5020-16408 2.1, 3.0 4.0 x 20 4.0, 4.6 IN5020-20384 IN5020-20333 IN5020-20376 IN5020-20325 - - - - IN5020-20401 IN5020-20350 IN5020-20393 IN5020-20342 IN5020-16506 IN5020-16505 IN5020-16405 IN5020-16404 -- - - IN5020-16504 IN5020-16503 IN5020-16403 IN5020-16402 -- - - IN5020-16514 IN5020-16513 IN5020-16413 IN5020-16412 -- - - IN5020-16512 IN5020-16511 IN5020-16411 IN5020-16410 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-21 LIFE SCIENCE

Colonnes HPLC/UHPLC InertSustain® Phenylhexyl Colonnes HPLC/UHPLC Physical Properties H3C O C on dition s ： GL-7700 S ystem 4000 QTRAP H N C H3 Column Dextromethorphan E luent ： 3 μm, 150 x 2.1 mm I.D. Flow rate ： A) CH3CN Silica : ES Silica Gel Col. Temp. Detection B) 10 mM CH3COONH4 in H2O Particle Size : 3 μm, 5 μm A/B = 30/70 (gradient mixer ), v/v I njection Vol. ：200 μL/min Surface Area : 350 m2/g 3.0e6 S ample ：40 C 2.0e6 ：S I M (ES I , Positive ) Pore Size : 100 Å (10 nm) 1.0e6 Max. 2.3e6 cps. 3.0e6 CUR I S TEM GS1 GS2 ihe 2.0e6 10 5500 500 60 40 ON Pore Volume : 0.85 mL/g 1.0e6 ：1 μL ：Dextromethorphan (50 ng/mL) Max. 1.7e6 cps. Bonded Phase : Phenylhexyl Groups End-capping : Yes 0.0 2 4 6 8 10 12 14 16 18 0.0 2 4 6 8 10 12 14 16 18 Time (min) Time (min) Carbon Loading : 9.0 % I nertSustain Phenylhexyl Other Brands Phenyl Column USP Code : L11 pH Range : 1-10 InertSustain Phenylhexyl columns are bonded with phenylhexyl groups, which employs a phenyl ring with a hexyl (6-carbon) linker and is densely bonded to ES silica gel delivering complementary selectivity to straight alkyl-chain columns, but with industry leading inertness, lot-to-lot reproducibility and low back pressure. Comparison of Selectivity with other brands 2 2,3 7 2,3 1 46 5 16 7,5 1 3 46 45 7 0 10 20 30 0 10 20 30 0 10 20 30 Time(min) Time(min) Time(min) InertSustain Phenylhexyl XBridge Phenyl Luna Phenyl-Hexyl Conditions Column Size : 5 μm, 150 × 4.6 mm I.D. Sample : 1. Uracil 2. Caffeine Eluent : A) CH3OH 3. Phenol 4. Butylbenzene B) H2O 5. o-Terphenyl 6. Amylbenzene A/B = 70/30, v/v 7. Triphenylene Flow Rate : 1.0 mL / min Col. Temp. : 40 °C Detection : UV 254 nm Analysis of Acidic Compounds 2 2 2 3 3 1 3 1 10 0 10 20 0 10 1 0 Time(min) 20 Time(min) Time(min) 20 Luna Phenyl-Hexyl InertSustain Phenylhexyl XBridge Phenyl Conditions Column Size : 5 μm, 150 × 4.6 mm I.D. Sample : 1. Brilliant Blue FCF 2. Phenol Eluent : A) CH3CN 3. Salicylic acid B) 0.1% H3PO4 A/B = 25/75, v/v Flow Rate : 1.0 mL/min Col. Temp. : 40 °C Detection : UV 254 nm E-22 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns InertSustain Phenylhexyl InertSustain® Phenylhexyl Dimensions 3 μm HP Series 3 μm 5 μm Guard Column E (200 bar) HPLC/UHPLC Columns (500 bar) (200 bar) (200 bar) ID x Length (in mm) IN5020-89043 1.0 x 250 - IN5020-89165 IN5020-89042 1.0 x 150 IN5020-89041 1.0 x 100 - IN5020-89164 IN5020-89040 1.0 x 75 IN5020-89039 1.0 x 50 - IN5020-89163 IN5020-89038 1.0 x 30 IN5020-89006 2.1 x 250 - IN5020-89162 IN5020-89005 2.1 x 150 IN5020-89004 2.1 x 100 - IN5020-89161 IN5020-89003 2.1 x 75 IN5020-89002 2.1 x 50 - IN5020-89160 IN5020-89001 2.1 x 30 IN5020-89013 3.0 x 250 IN5020-89214 IN5020-89129 IN5020-89012 3.0 x 150 IN5020-89011 3.0 x 100 IN5020-89213 IN5020-89128 IN5020-89010 3.0 x 75 IN5020-89009 3.0 x 50 IN5020-89212 IN5020-89127 IN5020-89008 3.0 x 30 IN5020-89020 4.0 x 250 IN5020-89211 IN5020-89126 IN5020-89019 4.0 x 150 IN5020-89018 4.0 x 100 IN5020-89210 IN5020-89125 IN5020-89017 Reusable holder with universal self adjusting 4.0 x 75 IN5020-89016 connections and cartridges containing the same 4.0 x 50 IN5020-89209 IN5020-89124 IN5020-89015 4.0 x 30 IN5020-89027 high performance packing material 4.6 x 250 IN5020-89220 IN5020-89136 IN5020-89026 4.6 x 150 IN5020-89025 4.6 x 100 IN5020-89219 IN5020-89135 IN5020-89024 IN5020-89023 IN5020-89218 IN5020-89134 IN5020-89022 IN5020-89217 IN5020-89133 IN5020-89216 IN5020-89132 IN5020-89215 IN5020-89131 UHPLC Guard Column (800 bar) - IN5020-89143 - IN5020-89142 Dedicated Holder - IN5020-89141 PEEK Guide Packing - IN5020-89140 - IN5020-89139 - IN5020-89138 IN5020-89226 IN5020-89150 Tubing IN5020-89225 IN5020-89149 Replacement Cartridges IN5020-89224 IN5020-89148 Simple to use cartridge system which includes a tubing installed to the holder offering 4.6 x 75 IN5020-89223 IN5020-89147 minimized dead volume. 4.6 x 50 IN5020-89222 IN5020-89146 4.6 x 30 IN5020-89221 IN5020-89145 InertSustain Phenylhexyl Guard Columns Dimensions 3 μm HP 3 μm HP 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 + 2 cartridges 2.1, 3.0 1.0 x 10 - - IN5020-89200 IN5020-89199 IN5020-89106 IN5020-89105 3.0 1.5 x 10 4.0, 4.6 2.1 x 10 IN5020-89230 IN5020-89227 IN5020-89202 IN5020-89201 IN5020-89108 IN5020-89107 2.1, 3.0 3.0 x 10 4.0, 4.6 4.0 x 10 IN5020-89231 IN5020-89228 - - - - 3.0 x 20 4.0 x 20 IN5020-89232 IN5020-89229 IN5020-89198 IN5020-89197 IN5020-89104 IN5020-89103 - - IN5020-89196 IN5020-89195 IN5020-89102 IN5020-89101 - - IN5020-89206 IN5020-89205 IN5020-89112 IN5020-89111 - - IN5020-89204 IN5020-89203 IN5020-89110 IN5020-89109 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-23 LIFE SCIENCE

Colonnes HPLC/UHPLC InertSustain® Cyano Colonnes HPLC/UHPLC InSei rtSustaCin CNyano Physical Properties : High Purity ES Silica Gel : 3 μm, 5 μm Silica InertSustain CyanoParticle Size S●uBrfaasceeMAarteerai al ::3H5ig0hmP2u/rgity ES Silica Gel evethteroatl,odrespsteaprdeboql●●oteourBPP.PB●●●●●airrooMoaeaniPFPPSnrsrrsndatugeeooaueoidncnrrrrrlMaeVSyvelteefceiaepiaodctCbSVzcSirtlleatooeeioyeuPhiztzn raldceeShmAuieanauhsmiarllozce-eesGetiea obpeprl-h oreboau.::abspH3tlec:::eiμ:::::hgmC10tmoC1033ho.0y0s.58,yμ8Pbra0005aa55meulnonÅmnmÅμro,mutoidm-t2L5(spp(yt/1oLga1/eμrr0gE/-rodo0melgSnop.pnmtdy(SyEmrle)ilelxsipc):igarUonGSdeeuPdlcuLib1si0ilni)tgy Si C N ● ES●nuErdfn-acdca-ecpAappreipnaign g : 35::0YYemess2/g ● CPP●●aooCUrrreebSarSVoPboinzoCleunLomodLeeaodadiningg :: 100.:8::085L8Åm1%%0L(1/0g nm) ● Si C N ons ● UF●uSpnPHctCiRooandnaegl G e roup : C:y:La21n-o07p.r5opyl 4A1A5.0/)0μBC°mmC=H,L●●●●132C/55m0pINCUpE/tIin7isnhn×HHn5Sase,4grdgPBuvR.bR6em)-e/evcoCa0nmasno.anne1omnseapgrd%LtarIgpre.eeDmoalHei,.na3olo tP,gdhcOdtiece4nhursgerntraiLsnb::::CtgilaL82Yitac1%:eby-to0s2i7amllu.-in5t7madyn.n5ryaetlnepacrdbohodnrrueoacltpooibrgrioilyeitdsya.uvToachfieiltabhIbeinllieeCtrypytSaroonuvfositdtaphinihnegaCsCtehyyeaaamnvnoatoioclaobpbluelhemeainnxstsetrheweamevmeraelayidlraikenebvetelreatl,orespinteapdbtohloteore.arMemnsdaoanrlreyvkepberatohttdceauhsrc-eetiobppl-reboo.abotlcerhm. oMsraalonntd-ytoab-reloatdtrceehspi-grtonode-udbcuaibstiilcnithgy Size : or lot-to- : te p. : : n : 1U.V42-H8Iy0ndnlrTgoomxehtynebreeeInrnazpeal,mrrttoihdSedeuussttcaaibibniliiCtlyityayannidossrceuopelruosmdauncrseibaiolrietcyhcoiufgtrhhrleyinCrgeycaaontmommpheaannsdyeeadlavfaboirloaarblalletpoihnratihremesam.cTaorhpkeeeitaIanmreeerpttohSoourd.ssMtraaeinqnyubCiraiyntcaghna-tooC-cyboaantlcuohmpohrnalsoste-wtoteo-lrobeterdeupserevoded.ul(ocEipbxie:litUdyStPo Lr1e0s)olve these : 2. H4-yHdiysrodspqouurroeinoxsoybbnaeelrneezomoiccsaccuaidrnrindgaartemdaneysliagbnoeradtouriessin.TghethIneermtSoussttaimn CoydaenorncoLluCmcnoslwuemrendteeveclhopneodlotogryesaovlvaeiltahebslee pproroblveimdsinagndthareemdestoignbeed uesxintrgemely inert, stable and 3. 4. PhethnoerFlemipgorusotrdmeuo1cdieb:rnlCeL.oCTmchopeluamInrniestreotcSnhunoostlfoagSiyneaClveaycialatnbiovleicptyorolvuidminngsthaermetohibgehelxytrreemceolyminmeret,nstdaeblde afondr raelplrpodhuacribmlea. copeia methods requiring a Cyano phase to be 5. 4p--HNTyitdhruooeprsohIIxeennyneedborerlt.nStSz(uoEusnstxitaot:iarniliUnCSyCaPynaoLn1o0c)olumns are highly recommended for all pharmacopeia methods requiring a Cyano phase to be used. (Ex: USP L10) 6. Conditions FiCgoumrep1ar:isCoonmopf3aSreisleocntivoiftySelectivity Column Size : 5 μm,150 × 4.6 mm I.D. Eluent : A) CH3CN B) 0.1 % H3PO4 InertSustain Cyan1o2 A/B = 25/75, v/v 3 45 6 ConditioFnlosw Rate : 1.0 mL/min Column SDCizoeetl.e::TcetA5imo)μnCpm.H,13C50::N4U×0V4B°.26C) 80m0.1mn%mI.DH.3PO4 Eluent SamplAe/B = 25:/715.,4v-/Hv ydroxybenzamide 0 12 4 6 8 10 Flow Rate : 1.0 mL/mi2n. Hydroquinone Time(min) 10 2 45 6 Col. Temp. : 40 °C 3. 4-Hydoroxybenzoic acid InertSustain Phenyl Detection : UV 280 nm4. Phenol : 1. 4-Hydro5x.y4b-eHnyzdaomriodxeybenzonitoril 02468 Sample 2. Hydroqu6i.npon-Neitrophenol 3 Time(min) 3. 4-Hydoroxybenzoic acid InertSustain Phen1yl 4. Phenol 2 4+5 6 5. 4-Hydoroxybenzonitoril 3 6. p-Nitrophenol 0 12 468 10 2 4+T5ime(min) 6 InertSustain C18 024 6 8 10 Time(min) 3 45 6 InertSustain C118 2 Condition3s Col1umn Size : 5 μm,150 × 4.6 mm I.D. 0 Eluen2t 2 : A) CH3C4 N B) 0.1% H63PO4 8 10 6 A/B = 25/75, v/Tvime(4m5in) 3 Flow Rate : 1.0 mL/min 8 0 Col.2Temp. : 404°C 6 10 Time(min) Detection : UV 230 nm CFoigmupraSearm2isploe: nCof:m1B.pUarasaciriclisCoonmopfoBuansdiscACnoamlyspios unds Analysis 2. Dextromethorphan Figure 2 : Com3pa3.rBiesrboenrineof Basic Compounds Analysis Conditions Column Size : 5 μm,150 × 4.6 mm I.D. 3 3 ConditionEsluent : A) CH3CN B) 0.1% H3PO4 2 A/B = 25/75, v/v 2 3 Column SizFelo:w5Rμamte,150 :×14..06 mmLm/mI.iDn. 68 10 2 13 Eluent Col:. ATe) mCHp.3CN: 4B0)°0C.1% H3PO4 min) 1 Dextromethorphan 2 2 DeteAct/iBon= 25/7:5U, vV/v230 nm 1 Berberine 1 3 Flow Rate Sam: 1p.l0e mL/mi:n 1. Uracil SS CN 1 2 Col. Temp. : 40 °C 1 Detection : UV 230 nm 2. Dextromethorphan Sample : 1. Uracil 3. Berberine 2. Dextromethorphan 3. Berberine 0 2 4 6 8 10 0 2 4 6 8 10 0 2 4 6 8 10 Time(min) Time(min) Time(min) 0 2InertS4uTismtea(minin6)Cyano8 10 0 2 4ZTiOmeR(mBin6A) X SB8-CN 10 0 2 4TimXeS(mein6l)ect HS8 S CN10 InertSustain Cyano ZORBAX SB-CN XSelect HSS CN Dextromethorphan Berberine E-24 CANADIAN 1 888-226-2775 ::[email protected]:in:e www.lifescience.ca LIFE SCIENCE

InertSustain Cyano HPLC/UHPLC Columns ● Base Material : HiIgnherPtSuuristtyaiEnSCSyailnicoa Gel InertSustain® Cyano IDDx●●imLeSPnegaunthrrst(fiiianoccmnleems)SAirzeea3 μ(m50H0::P33bS5aμe0rm)rmie, s25/gμm 3 μm 5 μm Si C N HPLC/UHPLC Columns PPooxrree (200 bar) (200 bar) ● SV25oiz0leume ::-010.805ÅmL(1/0g nm) IN5020-87415 Guard Column E (200 bar) 1.0● IN5020-89293 IN5020-89292 1.0● Fuxnct1io50nal Group :-Cyanopropyl IN5020-87414 IN5020-89291 IN5020-89290 1.0● Enxd-c1a0p0 ping :- Yes IN5020-87413 IN5020-89289 IN5020-89288 1.0● Caxrbo7n5 Loading :- 8% IN5020-87412 1.0●● UpHSx PRCa5no0gdee ::- L21-07.5 IN5020-87411 1.0 x 30 - IN5020-87410 2.1In gexner2a5l0, the staIbNi5li0t2y0-a8n94d64reproducibIiNlit5y02o0-f8t9h37e9Cyano phaINs5e02a0v-8a9i2la56ble in the market are poor. Many batch-to-batch or lot-to-lot reproducibility 2.1issuex s a1re50occurrinINg50a20t -m89a46n3y laboratorIiNe5s0.2T0h-8e93I7n8ertSustainINC5y0a20n-8o92c5o5lumns were developed to resolve these problems and are designed using 2.1the mx ost10m0 odern LINC50c20o-l8u9m46n2 technologINy5a02v0a-8il9a3b77le providingINt5h0e20m-89t2o54be extremely inert, stable and reproducible. The InertSustain Cyano columns are highly recommended for all pharmacopeia methods requiring a Cyano phase to be used. (Ex: USP L10) 2.1 x 75 IN5020-89461 IN5020-89376 IN5020-89253 2.1 x 50 IN5020-89460 IN5020-89375 IN5020-89252 2.1Figxure310 : ComIN5p0a20r-8i9s45o9n of SeleINc50t2i0v-8i9t3y74 IN5020-89251 IN5020-89263 Reusable holder with universal self adjusting 3.0 InexrtSus2t5a0in CyanoIN5020-89470 IN5020-89262 Conditions connections and cartridges containing the same IN5020-89386 IN5020-89261 IN5020-89260 Column Size : high5 μm,150 × 4.6 0mp.1me%rI.fDHo.3PrmO4 ance 3.0 x 150 3IN5020-89469 IN5020-89385 10 IN5020-89259 Eluent : packing material IN5020-89258 A) CH3CN B) IN5020-89270 3.0 x 100 1 IN5020-89468 IN5020-89384 IN5020-89269 A/B = 25/75, v/v 3.0 x 75 2 IN5020-8495467 IN5020-89383 IN5020-89268 6 IN5020-89267 Flow Rate : 1.0 mL/min 10 IN5020-89266 IN5020-89265 Col. Temp. : 40 °C IN5020-89277 Detection : UV 280 nm 3.0 0 x 50 2 IN5020-489466 6 IN5020-898382 Sample : 1. 4-Hydroxybenzamide IN5020-89381 Time(min) IN5020-89393 Did you know?2. Hydroquinone 3.0 x 30 IN5020-89465 3. 4-Hydoroxybenzoic acid InertSustain Phenyl 4. Phenol 4.0 x 250 - 5. 4-Hydoroxybenzonitoril 4.0 x 150 3- IN5020-89392 6. We sellp-Nitrophenol Gas Chromatography 4.0 x 100 1 - IN5020-89391 Columns & Accessories? 2 - 4+5 IN5020-689390 For more information please 4.0 x 75 ask your Sales Representative today. 4.0 0 x 50 2 -4 6 IN5020-898389 IN5020-89388 4.0 x 30 - Time(min) IN5020-89400 InertSustain C18 4.6 x 250 IN5020-89476 4.6 x 150 3IN5020-89475 IN5020-89399 IN5020-89276 IN5020-89398 4.6 x 100 1 2 IN5020-89474 4 5IN5020-89397 IN5020-89275 6 IN5020-89274 4.6 x 75 IN5020-89473 4.6 0 x 50 2 IN5020-489472 6 IN5020-898396 10 IN5020-89273 4.6 x 30 IN5020-89395 IN5020-89272 Time(min) IN5020-89471 Figure 2 : Comparison of Basic Compounds Analysis 3 Conditions Column Size : 5 μm,150 × 4.6 mm I.D. 3 Eluent : A) CH3CN B) 0.1% H3PO4 2 2 A/B = 25/75, v/v 1 1 3 Flow Rate : 1.0 mL/min Dimensions InertSustain Cyano Guard Co2 lumns Col. Temp. : 40 °C ID x Length (mm) 1 Detection : UV 230 nm 3 μm 3 μm 5 μm Sample : 1. Uracil Replacement (Guard Column E Set) (Guard Column E Set) 2. Dextrom5etμhomrphan Column *Includes 1 holder Cartridges *Includes 1 holder 3. BeRrbeeprinlaecement ID (mm) + 2 cartridges + 2 cartridges Cartridges 1.0 x 10 IN5020-89450 IN5020-89449 0 IN5020-89356 IN5020-89355 1.0 8 10 0 IN5020-89357 1.5, 2.1 0246 2 4 6 8 10 2 4 6 8 10 IN5020-89353 IN5020-89452 DextromethorpINha5n020-89351 3.0 1.5 x 10 Time(min) Time(min) IN5020-89451 Time(minIN) 5020-89358 Berberine 4.0, 4.6 3.0 xInertS10ustain Cyano IN5030-89448 ZORBAX SBI-NC5N020-89447 XSelect HSISN5C02N0-89354 4.0 x 10 IN5020-89446 IN5020-89445 IN5020-89352 3.0 x 20 IN5020-89456 IN5020-89455 IN5020-89362 IN5020-89361 2.1, 3.0 4.0 x 20 IN5020-89454 IN5020-89453 IN5020-89360 IN5020-89359 4.0, 4.6 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-25 LIFE SCIENCE

Colonnes HPLC/UHPLC InertSustain® NH2 Colonnes HPLC/UHPLC 105 InertSustain NH2 Other Brands NH2 Column A Other Brands NH2 Column B 100 Physical Properties Initial retention time (%) 95 Silica : ES Silica Gel 90 Particle Size : 3 μm, 5 μm Surface Area : 350 m2/g 85 Pore Size : 100 Å Stability comparison between 80 Pore Volume : 0.85 mL/g various amino columns Bonded Phase : Aminopropyl Groups 75 0 1000 2000 3000 4000 5000 6000 End-capping : None Volume of eluent passing through the column (mL) Carbon Loading : 7% Conditions Sample:1. Maltose USP Code : L8 Column Size : 5 μm, 250 × 4.6 mm I.D. Flow Rate : 1.0 mL/min pH Range : 2-7.5 Col. Temp. : 40 °C Detection : RI InertSustain NH2 shows far superior stability compared to the other brand available aminopropyl columns, as the “Evolved Surface Silica” is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are difficult to separate in reversed-phase mode, such as the analysis of sugars or vitamin E. InertSustain NH2 delivers highly reliable reproducibility and stability with accurate qualitative and quantitative results. Furthermore, aminopropyl columns generally cannot be washed by a weakly acidic eluent, however InertSustain NH2, being an improved phase, can be washed by a weakly acidic eluent. Analysis of Sugars Conditions System: GL-7400 HPLC system RIƒUÊRIU 3 5 Column: InertSustain NH2 (5 μm, 250 x 4.6 mm I.D.) 20 40 6 Column Cat. No. : IN5020-16628 4 7 Eluent: 12 BAA)/)BHCH2=O38C5N/15, v/v Flow Rate: 1.0 mL/min 0 Col. Temp.: 40°C Detection: RI Injection Vol.: 10 μL Sample: Standard Analyte: 1. Rhamnose 2. Fucose 3. Fructose 4. Glucose 5. Sucrose 6. Maltose 7. Lactose 0 10 20 Time (min) E-26 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Dimensions Inertsil NH2 5 μm HPLC/UHPLC Columns HPLC/UHPLC Columns 3 μm (200 bar) ID x Length (in mm) (200 bar) InertSustain® NH2 IN5020-16644 1.0 x 250 IN5020-16773 IN5020-16643 Guard Column E (200 bar) 1.0 x 150 IN5020-16772 IN5020-16642 1.0 x 100 IN5020-16771 IN5020-16641 Reusable holder with universal self adjusting 1.0 x 75 IN5020-16770 IN5020-16640 connections and cartridges containing the same 1.0 x 50 IN5020-16769 IN5020-16639 1.0 x 30 IN5020-16768 IN5020-16607 high performance packing material 2.1 x 250 IN5020-16737 IN5020-16606 2.1 x 150 IN5020-16736 IN5020-16605 Did you know? 2.1 x 100 IN5020-16735 IN5020-16604 2.1 x 75 IN5020-16734 IN5020-16603 We sell a wide range of HPLC maintenance 2.1 x 50 IN5020-16733 IN5020-16602 products such as Pump Seals, Check Valves, 2.1 x 30 IN5020-16732 IN5020-16614 3.0 x 250 IN5020-16744 IN5020-16613 Piston Seals and Lamps. 3.0 x 150 IN5020-16743 IN5020-16612 3.0 x 100 IN5020-16742 IN5020-16611 For more information please 3.0 x 75 IN5020-16741 IN5020-16610 ask your Sales Representative today. 3.0 x 50 IN5020-16740 IN5020-16609 3.0 x 30 IN5020-16739 IN5020-16621 NEXT DAY DELIVERY! 4.0 x 250 IN5020-16751 IN5020-16620 4.0 x 150 IN5020-16750 IN5020-16619 4.0 x 100 IN5020-16749 IN5020-16618 4.0 x 75 IN5020-16748 IN5020-16617 4.0 x 50 IN5020-16747 IN5020-16616 4.0 x 30 IN5020-16746 IN5020-16628 4.6 x 250 IN5020-16758 IN5020-16627 4.6 x 150 IN5020-16757 IN5020-16626 4.6 x 100 IN5020-16756 IN5020-16625 IN5020-16755 IN5020-16624 4.6 x 75 IN5020-16754 IN5020-16623 IN5020-16753 4.6 x 50 4.6 x 30 Inertsil NH2 Guard Columns Dimensions 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement ID (mm) ID x Length (mm) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 IN5020-16706 1.0 x 10 IN5020-16808 IN5020-16807 IN5020-16707 IN5020-16708 3.0 1.5 x 10 IN5020-16810 IN5020-16704 4.0, 4.6 3.0 x 10 IN5020-16806 IN5020-16809 IN5020-16709 IN5020-16702 2.1, 3.0 4.0 x 10 IN5020-16804 IN5020-16712 4.0, 4.6 3.0 x 20 IN5020-16814 IN5020-16805 IN5020-16705 IN5020-16710 4.0 x 20 IN5020-16812 IN5020-16803 IN5020-16703 IN5020-16813 IN5020-16713 IN5020-16811 IN5020-16711 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-27 LIFE SCIENCE

Colonnes HPLC/UHPLC InertSustain® Amide Colonnes HPLC/UHPLC Retention Mechanism in HILIC Mode Mobile Phase ACN H2O Physical Properties Silica : ES Silica Gel Analyte ＋ Particle Size : 1.9 μm, 3 μm, 5 μm Analyte Surface Area : 350 m2/g Hydration Layer Pore Size : 100 Å (10 nm) Hydrogen H2O Bonding Pore Volume : 0.85 mL/g OH OH OH O- O Si Bonded Phase : Carbamoyl Groups -O O H SHi O Si O Si O Si O Si O Si O OO O O Si OO Si O End-capping : No Si O O O OO Si O Si Si O O Carbon Loading : 15 % Si Si O OO O Si O Si O O OO USP Code : L68 OO pH Range : 2-8.5 HILIC (Hydrophilic-Interaction Chromatography) column for enhanced retention of extremely polar compounds. This InertSustain offers the strongest retentivity among the Amide columns available in the market due to the usage and bonding of carbamoyl groups. InertSustain AMIDE offers superior stability and durability even when using water-rich mobile phases. Comparison of retention between commercially available HILIC columns InertSustain Amide 2 Conditions 1 Column ： 5 μm, 150 × 2.1 mm I.D 3 4 Eluent ： A) CH3CN B) 10 mM HCOONH4 0 10 20 A/B = 90/10, v/v Time (min) 20 Flow Rate ： 0.2 mL/min 2 20 Col. Temp.： 40 ℃ 1 3 20 Detection ： UV 215 nm Sample ：1. Cyanuric Acid TSKgel Amide-80 2 4 (Amide) 2. Melamine 3. Ammelide 4. Ammeline 0 10 Time (min) XBridge BEH Amide (Amide) 3 4 0 1 Atlantis Silica HILIC (Unbonded Silica) 3 10 1 Time (min) 0 2 4 10 Time (min) 1st Injection Conditions ： InertSustain Amide (5 μm, 150 × 3.0 mm I.D) Column Eluent ： A) CH3CN B) 0.1% H3PO4 A/B = 87/13, v/v 1 Flow Rate ： 0.8 mL / min Col. Temp. ： 40 ℃ Detection ： UV 243 nm Injection ： 2 μL Vol. E-28 1.0 2.0 1 8838.-0226-2775 Sam::pleinfo@：li1fe. Assccioernbiccea.cciiad :: www.lifescience.ca CANADIANTime (min) Diluent : 2 % metaphosphoric acid aqueous solution LIFE SCIENCE

HPLC/UHPLC Columns Dimensions InertSustain Amide 5 μm InertSustain® Amide HPLC/UHPLC Columns 3 μm (200 bar) ID x Length (in mm) (200 bar) Guard Column E (200 bar) IN5020-88647 1.0 x 250 IN5020-88771 IN5020-88646 Reusable holder with universal self adjusting 1.0 x 150 IN5020-88770 IN5020-88645 connections and cartridges containing the same 1.0 x 100 IN5020-88769 IN5020-88644 1.0 x 75 IN5020-88768 IN5020-88643 high performance packing material 1.0 x 50 IN5020-88767 IN5020-88642 1.0 x 30 IN5020-88766 IN5020-88608 Did you know? 2.1 x 250 IN5020-88732 IN5020-88607 Canadian Life Science is an 2.1 x 150 IN5020-88731 IN5020-88606 Authorized Distributor for 2.1 x 125 IN5020-88730 IN5020-88605 Honeywell Research Chemicals? 2.1 x 100 IN5020-88729 IN5020-88604 2.1 x 75 IN5020-88728 IN5020-88603 For more information please 2.1 x 50 IN5020-88727 IN5020-88602 ask your Sales Representative today. 2.1 x 30 IN5020-88726 IN5020-88616 3.0 x 250 IN5020-88740 IN5020-88615 3.0 x 150 IN5020-88739 IN5020-88614 3.0 x 125 IN5020-88738 IN5020-88613 3.0 x 100 IN5020-88737 IN5020-88612 3.0 x 75 IN5020-88736 IN5020-88611 3.0 x 50 IN5020-88735 IN5020-88610 3.0 x 30 IN5020-88734 IN5020-88624 4.0 x 250 IN5020-88748 IN5020-88623 4.0 x 150 IN5020-88747 IN5020-88622 4.0 x 125 IN5020-88746 IN5020-88621 4.0 x 100 IN5020-88745 IN5020-88620 4.0 x 75 IN5020-88744 IN5020-88619 4.0 x 50 IN5020-88743 IN5020-88618 4.0 x 30 IN5020-88742 IN5020-88632 4.6 x 250 IN5020-88756 IN5020-88631 4.6 x 150 IN5020-88755 IN5020-88630 4.6 x 125 IN5020-88754 IN5020-88629 4.6 x 100 IN5020-88753 IN5020-88628 4.6 x 75 IN5020-88752 IN5020-88627 4.6 x 50 IN5020-88751 IN5020-88626 4.6 x 30 IN5020-88750 InertSustain Amide Guard Columns Dimensions 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement ID (mm) ID x Length (mm) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 IN5020-88709 1.0 x 10 IN5020-88806 IN5020-88805 IN5020-88710 IN5020-88711 3.0 1.5 x 10 IN5020-88808 IN5020-88707 4.0, 4.6 3.0 x 10 IN5020-88804 IN5020-88807 IN5020-88712 IN5020-88705 2.1, 3.0 4.0 x 10 IN5020-88802 IN5020-88715 4.0, 4.6 3.0 x 20 IN5020-88812 IN5020-88803 IN5020-88708 IN5020-88713 4.0 x 20 IN5020-88810 IN5020-88801 IN5020-88706 IN5020-88811 IN5020-88716 IN5020-88809 IN5020-88714 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-29 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® ODS-4V (Validated for GLP/GMP Compliance) Inertsil® ODS-4 Colonnes HPLC/UHPLC Physical Properties Physical Properties Silica : 3 Series High Purity Silica Gel Silica : 3 Series High Purity Silica Gel Particle Size : 2 μm, 3 μm, 5 μm Particle Size : 3 μm, 5 μm Surface Area : 450 m2/g Surface Area : 450 m2/g Pore Size : 100 Å Pore Size : 100 Å Pore Volume : 1.05 mL/g Pore Volume : 1.05 mL/g Bonded Phase : Octadecyl Groups Bonded Phase : Octadecyl Groups End-capping : Yes End-capping : Yes Carbon Loading : 11% Carbon Loading : 11% USP Code : L1 USP Code : L1 pH Range : 2-7.5 pH Range : 2-7.5 Inertsil ODS-4 delivers the same extreme inertness to any Each Inertsil ODS-4V is delivered with a Manufacturer’s type of compound just like InertSustain C18 along with Validation Certificate showing the detailed results of every QA unprecedented stability under 100% aqueous mobile phases and QC step in manufacturing. By choosing Inertsil ODS-4V, you for qualitative and quantitative analysis. However, as the base can be assured that you are using one of the most trusted and silica gel and carbon loading are different on Inertsil ODS-4, enduring HPLC columns for validation. differences in selectivity can be observed for certain analytes. Figure 1. Analysis of Haloacetic Acids by LC-MS/MS Dimensions Inertsil ODS-4V 5 μm 3 μm (200 bar) System : LC800 LC system Detection: LC-MS/MS(ESI,Negative,MRM) ID x Length (in mm) (200 bar) 4000 Q TRAP® Injection Vol. : 10 µL - Column : Inertsil ODS-4 3µm, 150mm × 2.1mm 2.1 x 250 IN5020-30216 - Eluent : A) C0.H13%OHHCOOH 2.1 x 150 IN5020-30215 - B) A/B = 5/95 - 2.5 min- 5/95 - 2.5 min ‒ 95/5 - 5 min- 95/5 2.1 x 100 IN5020-30214 - Flow Rate : 0.2 mL/min 2.1 x 75 IN5020-30213 - Col. Temp. : 40°C 2.1 x 50 IN5020-30212 IN5020-10922 3.0 x 250 IN5020-30226 IN5020-10921 Sample: 3.0 x 150 IN5020-30225 - 1. Bromoacetic acid 3.0 x 100 IN5020-30224 - 2. Dibromoacetic acid 3.0 x 75 IN5020-30223 - 3. Tribromoacetic acid 3.0 x 50 IN5020-30222 IN5020-10912 4. Chloroacetic acid 4.0 x 250 IN5020-30236 IN5020-10911 5. Dichloroacetic acid 4.0 x 150 IN5020-30235 - 6. Trichloroacetic acid 4.0 x 100 IN5020-30234 - 7. Bromochloroacetic acid 4.0 x 75 IN5020-30233 - 4.0 x 50 IN5020-30232 IN5020-10902 4.6 x 250 IN5020-30246 IN5020-10901 4.6 x 150 IN5020-30245 - 4.6 x 100 IN5020-30244 - 4.6 x 75 IN5020-30243 - 4.6 x 50 IN5020-30242 E-30 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Dimensions 2 μm Inertsil ODS-4 3 μm 5 μm Inertsil® ODS-4/ODS-4V HPLC/UHPLC Columns (800 bar) 3 μm HP Series (200 bar) (200 bar) ID x Length (in mm) Guard Column E (200 bar) - (500 bar) IN5020-81116 IN5020-81016 1.0 x 250 - IN5020-81115 IN5020-81015 Reusable holder with universal self adjusting 1.0 x 150 - - IN5020-81114 IN5020-81014 connections and cartridges containing the same 1.0 x 100 - - IN5020-81113 IN5020-81013 1.0 x 75 - - IN5020-81112 IN5020-81012 high performance packing material 1.0 x 50 - - IN5020-81111 IN5020-81011 1.0 x 30 - - IN5020-04016 IN5020-03916 UHPLC Guard Column (800 bar) 2.1 x 250 IN5020-81205 - IN5020-04015 IN5020-03915 2.1 x 150 - IN5020-14003 IN5020-04017 IN5020-03917 Dedicated Holder 2.1 x 125 IN5020-81204 IN5020-14002 IN5020-04014 IN5020-03914 2.1 x 100 IN5020-81203 - IN5020-04013 IN5020-03913 PEEK Guide Packing 2.1 x 75 IN5020-81202 IN5020-14001 IN5020-04012 IN5020-03912 2.1 x 50 IN5020-81200 IN5020-14063 IN5020-04011 IN5020-03911 Tubing 2.1 x 30 - IN5020-14062 IN5020-04026 IN5020-03926 3.0 x 250 IN5020-81215 IN5020-14061 IN5020-04025 IN5020-03925 Replacement Cartridges 3.0 x 150 - IN5020-14006 IN5020-04027 IN5020-03927 3.0 x 125 IN5020-81214 IN5020-14005 IN5020-04024 IN5020-03924 Simple to use cartridge system which includes 3.0 x 100 IN5020-81213 - IN5020-04023 IN5020-03923 a tubing installed to the holder offering 3.0 x 75 IN5020-81212 IN5020-14004 IN5020-04022 IN5020-03922 minimized dead volume. 3.0 x 50 IN5020-81210 IN5020-14066 IN5020-04021 IN5020-03921 3.0 x 30 - IN5020-14065 IN5020-04036 IN5020-03936 4.0 x 250 - IN5020-14064 IN5020-04035 IN5020-03935 4.0 x 150 - - IN5020-04037 IN5020-03937 4.0 x 125 - - IN5020-04034 IN5020-03934 4.0 x 100 - - IN5020-04033 IN5020-03933 4.0 x 75 - - IN5020-04032 IN5020-03932 4.0 x 50 - - IN5020-04031 IN5020-03931 4.0 x 30 - - IN5020-04046 IN5020-03946 4.6 x 250 - - IN5020-04045 IN5020-03945 4.6 x 150 - IN5020-14009 IN5020-04047 IN5020-03947 4.6 x 125 - IN5020-14008 IN5020-04044 IN5020-03944 4.6 x 100 - - IN5020-04043 IN5020-03943 4.6 x 75 - IN5020-14007 IN5020-04042 IN5020-03942 4.6 x 50 - IN5020-14069 IN5020-04041 IN5020-03941 4.6 x 30 IN5020-14068 IN5020-14067 Inertsil ODS-4 Guard Columns Dimensions 2 μm 2 μm 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 + 2 cartridges IN5020-19201 2.1, 3.0 1.0 x 10 - IN5020-19252 IN5020-19202 IN5020-19251 IN5020-19301 1.5 x 10 - 3.0 2.1 x 10 IN5020-20360 IN5020-20309 IN5020-19352 IN5020-19302 IN5020-19351 - 4.0, 4.6 3.0 x 10 IN5020-20377 IN5020-19101 2.1, 3.0 4.0 x 10 IN5020-20394 IN5020-20326 - - - IN5020-19001 4.0, 4.6 3.0 x 20 IN5020-19501 4.0 x 20 - IN5020-20343 IN5020-19152 IN5020-19102 IN5020-19151 IN5020-19401 - - - IN5020-19052 IN5020-19002 IN5020-19051 - IN5020-19552 IN5020-19502 IN5020-19551 - IN5020-19452 IN5020-19402 IN5020-19451 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-31 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® C8-4 Colonnes HPLC/UHPLC 7 Physical Properties Silica : 3 Series High Purity Silica Gel 7 Particle Size : 2 μm, 3 μm, 5 μm 2 5+ 6 Shortening the 5+ 6 13 4 analysis time 4 Surface Area : 450 m2/g 2 Pore Size : 100 Å 13 Pore Volume : 1.05 mL/g Bonded Phase : Octyl Groups 0 10 20 0 2 4 6 8 10 Time (min) Time (min) End-capping : Yes Inertsil ODS-4 Inertsil C8-4 Carbon Loading : 5% USP Code : L7 pH Range : 2-7.5 Many chromatographers prefer a C8 column when an ODS phase shows excessive retention values. Inertsil C8-4 provides the same separation pattern (selectivity) and extreme inertness to any type of compounds just like Inertsil ODS-4, which enables easy method transfer from ODS-4 to C8-4 while other commercially available ODS and C8 columns can show dramatically different selectivity even though they are part of the same brand/series. Analysis of 14 kinds of Phthalates Conditions: Analyte: 1. Bis(2-methoxyethyl)phthalate(DMEP) 2 System: GL-7400 HPLC system 2. Dimethyl phthalate(DMP) 3. Diethyl Phthalate(DEP) 20 mAU 40 60 Column: Inertsil C8-4 4. Diisopropyl Phthalate(DIPP) (5 m, 250 x 4.6 mm I.D.) 5. Dipropyl Phthalate(DPrP) 6. Diphenyl Phthalate(DPhP) Column Cat. No.: IN5020-04086 7. Benzyl butyl Phthalate(BBP) 8. Diisobutyl Phthalate(DIBP) 1 3 45 6 7 Eluent: 9. Dipentyl Phthalate(DPeP) 10. Dihexyl Phthalate(DHxP) 8 A) HCH2O3CN 11. Diheptyl Phthalate(DHpP) B) 12. B is(2-ethylhexyl) Phthalate(DEHP) A/B = 60/40 – 20 min – 90/10 – 5 min 13. DioctylPhthalate(DOP) 9 13 – 90/10, v/v 14. Dinonyl Phthalate(DNP) 10 12 14 Flow Rate: 1.0 mL/min 11 Col. Temp.: 40 °C 0 Detection: UV 230 nm (GL-7450 UV Detector) Injection Vol.: 10 μL 0 10 20 Sample: Standard Time (min) E-32 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Dimensions 2 μm Inertsil C8-4 3 μm 5 μm Inertsil® C8-4 HPLC/UHPLC Columns (800 bar) 3 μm HP Series (200 bar) (200 bar) ID x Length (in mm) Guard Column E (200 bar) - (500 bar) IN5020-81266 IN5020-81226 1.0 x 250 - IN5020-81265 IN5020-81225 Reusable holder with universal self adjusting 1.0 x 150 - - IN5020-81264 IN5020-81224 connections and cartridges containing the same 1.0 x 100 - - IN5020-81263 IN5020-81223 1.0 x 75 - - IN5020-81262 IN5020-81222 high performance packing material 1.0 x 50 - - IN5020-81261 IN5020-81221 1.0 x 30 - - IN5020-03976 IN5020-04056 UHPLC Guard Column (800 bar) 2.1 x 250 IN5020-81285 - IN5020-03975 IN5020-04055 2.1 x 150 - IN5020-14053 IN5020-03977 IN5020-04057 Dedicated Holder 2.1 x 125 IN5020-81284 IN5020-14052 IN5020-03974 IN5020-04054 2.1 x 100 IN5020-81283 - IN5020-03973 IN5020-04053 PEEK Guide Packing 2.1 x 75 IN5020-81282 IN5020-14051 IN5020-03972 IN5020-04052 2.1 x 50 IN5020-81280 IN5020-14073 IN5020-03971 IN5020-04051 Tubing 2.1 x 30 - IN5020-14072 IN5020-03983 IN5020-04066 3.0 x 250 IN5020-81295 IN5020-14071 IN5020-03982 IN5020-04065 Replacement Cartridges 3.0 x 150 - IN5020-14056 IN5020-03984 IN5020-04067 3.0 x 125 IN5020-81294 IN5020-14055 IN5020-03981 IN5020-04064 Simple to use cartridge system which includes 3.0 x 100 IN5020-81293 - IN5020-03980 IN5020-04063 a tubing installed to the holder offering 3.0 x 75 IN5020-81292 IN5020-14054 IN5020-03979 IN5020-04062 minimized dead volume. 3.0 x 50 IN5020-81290 IN5020-14076 IN5020-03978 IN5020-04061 3.0 x 30 - IN5020-14075 IN5020-03990 IN5020-04076 4.0 x 250 - IN5020-14074 IN5020-03989 IN5020-04075 4.0 x 150 - - IN5020-03991 IN5020-04077 4.0 x 125 - - IN5020-03988 IN5020-04074 4.0 x 100 - - IN5020-03987 IN5020-04073 4.0 x 75 - - IN5020-03986 IN5020-04072 4.0 x 50 - - IN5020-03985 IN5020-04071 4.0 x 30 - - IN5020-03997 IN5020-04086 4.6 x 250 - - IN5020-03996 IN5020-04085 4.6 x 150 - IN5020-14059 IN5020-03998 IN5020-04080 4.6 x 125 - IN5020-14058 IN5020-03995 IN5020-04084 4.6 x 100 - - IN5020-03994 IN5020-04083 4.6 x 75 - IN5020-14057 IN5020-03993 IN5020-04082 4.6 x 50 - IN5020-14079 IN5020-03992 IN5020-04081 4.6 x 30 IN5020-14078 IN5020-14077 Inertsil C8-4 Guard Columns Dimensions 2 μm 2 μm 3 μm HP 3 μm HP 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 + 2 cartridges + 2 cartridges 2.1, 3.0 1.0 x 10 -- - - IN5020-19297 IN5020-19247 IN5020-19296 IN5020-19246 3.0 1.5 x 10 4.0, 4.6 2.1 x 10 IN5020-20361 IN5020-20310 IN5020-20356 IN5020-20305 IN5020-19397 IN5020-19347 IN5020-19396 IN5020-19346 2.1, 3.0 3.0 x 10 4.0, 4.6 4.0 x 10 IN5020-20378 IN5020-20327 IN5020-20373 IN5020-20322 - - - - 3.0 x 20 4.0 x 20 IN5020-20395 IN5020-20344 IN5020-20390 IN5020-20339 IN5020-19197 IN5020-19147 IN5020-19196 IN5020-19146 -- - - IN5020-19097 IN5020-19047 IN5020-19096 IN5020-19046 -- - - IN5020-19597 IN5020-19547 IN5020-19596 IN5020-19546 -- - - IN5020-19497 IN5020-19447 IN5020-19496 IN5020-19446 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-33 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® ODS-3V Inertsil® ODS-3 Colonnes HPLC/UHPLC Physical Properties Physical Properties Silica : 3 Series High Purity Silica Gel Silica : 3 Series High Purity Silica Gel Particle Size : 2 μm, 3 μm, 4 μm, 5 μm, 7 μm, 10 μm Particle Size : 3 μm, 5 μm Surface Area : 450 m2/g Surface Area : 450 m2/g Pore Size : 100 Å Pore Size : 100 Å Pore Volume : 1.05 mL/g Pore Volume : 1.05 mL/g Bonded Phase : Octadecyl Groups Bonded Phase : Octadecyl Groups End-capping : Yes End-capping : Yes Carbon Loading : 15% Carbon Loading : 15% USP Code : L1 USP Code : L1 pH Range : 2-7.5 pH Range : 2-7.5 Inertsil ODS-3 is still GL Sciences’ most popular phase and Each Inertsil ODS-3V is delivered with a Manufacturer’s continues to be widely used for long established methods in Validation Certificate showing the detailed results of every QA pharmaceutical and contract research labs. Inertsil ODS-3 has a and QC step in manufacturing. By choosing Inertsil ODS-3V, you relatively strong retentivity compared to commercially available can be assured that you are using one of the most trusted and ODS columns. In addition, the introduction of higher surface enduring HPLC columns for validation. area silica provides high preparative loading capacity without sacrificing peak shape. Dimensions Inertsil ODS-3V 5 μm Comparison of Retentivity 3 μm (200 bar) ID x Length (in mm) (200 bar) 27 - 13 4 56 2.1 x 250 IN5020-30116 - 2.1 x 150 IN5020-30115 - 0 10 20 30 40 2.1 x 100 IN5020-30114 - Time(min) 7 40 2.1 x 75 IN5020-30113 - Inertsil ODS-3 2.1 x 50 IN5020-30112 IN5020-01822 4 56 3.0 x 250 IN5020-30126 IN5020-01821 2 3.0 x 150 IN5020-30125 - 1 3.0 x 100 IN5020-30124 - 3.0 x 75 IN5020-30123 - 3 3.0 x 50 IN5020-30122 IN5020-01812 4.0 x 250 IN5020-30136 IN5020-01811 0 10 20 30 4.0 x 150 IN5020-30135 - Time(min) 4.0 x 100 IN5020-30134 - InertSustain C18 4.0 x 75 IN5020-30133 - 7 4.0 x 50 IN5020-30132 IN5020-01802 2 5,6 4.6 x 250 IN5020-30146 IN5020-01801 4.6 x 150 IN5020-30145 - 13 4 4.6 x 100 IN5020-30144 - 4.6 x 75 IN5020-30143 - 0 10 20 30 40 4.6 x 50 IN5020-30142 Time(min) Inertsil ODS-4 Conditions Sample : 1. Uracil Column Size : 5 μm, 250 × 4.6 mm I.D. 2. Caffeine 3. Phenol Eluent : A) CH3OH 4. Butylbenzene 5. o -Terphenyl B) H2O 6. Amylbenzene 7. Triphenylene A/B= 80/20, v/v Flow Rate : 1.0 mL/min Col.Temp. : 40 °C Detection : UV 254 nm E-34 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Dimensions 2 μm Inertsil ODS-3 4 μm 5 μm Inertsil® ODS-3/ODS-3V HPLC/UHPLC Columns (800 bar) 3 μm (200 bar) (200 bar) ID x Length (in mm) Guard Column E (200 bar) - (200 bar) - IN5020-13252 1.0 x 250 - - IN5020-13251 Reusable holder with universal self adjusting 1.0 x 150 - - - IN5020-84514 connections and cartridges containing the same 1.0 x 100 - IN5020-13360 - IN5020-84513 1.0 x 75 - IN5020-84414 - IN5020-84512 high performance packing material 1.0 x 50 - IN5020-84413 - IN5020-84511 1.0 x 33 - IN5020-84412 IN5020-04616 IN5020-01742 UHPLC Guard Column (800 bar) 2.1 x 250 IN5020-84655 IN5020-84411 IN5020-04615 IN5020-01741 2.1 x 150 - IN5020-04416 IN5020-04617 IN5020-04515 Dedicated Holder 2.1 x 125 IN5020-84654 IN5020-04415 IN5020-04614 IN5020-04514 2.1 x 100 IN5020-84653 IN5020-04417 IN5020-04613 IN5020-04513 PEEK Guide Packing 2.1 x 75 IN5020-84652 IN5020-04414 IN5020-04612 IN5020-04512 2.1 x 50 - IN5020-04413 IN5020-04611 IN5020-04511 Tubing 2.1 x 33 - IN5020-04412 IN5020-04626 IN5020-01752 3.0 x 250 IN5020-84665 IN5020-04411 IN5020-04625 IN5020-01751 Replacement Cartridges 3.0 x 150 - IN5020-04426 IN5020-04627 IN5020-04525 3.0 x 125 IN5020-84664 IN5020-04425 IN5020-04624 IN5020-04524 Simple to use cartridge system which includes 3.0 x 100 IN5020-84663 IN5020-04427 IN5020-04623 IN5020-04523 a tubing installed to the holder offering 3.0 x 75 IN5020-84662 IN5020-04424 IN5020-04622 IN5020-04522 minimized dead volume. 3.0 x 50 - IN5020-04423 IN5020-04621 IN5020-04521 3.0 x 33 - IN5020-04422 IN5020-04636 IN5020-01762 4.0 x 250 - IN5020-04421 IN5020-04635 IN5020-01761 4.0 x 150 - IN5020-04436 IN5020-04637 IN5020-01767 4.0 x 125 - IN5020-04435 IN5020-04634 IN5020-01766 4.0 x 100 - IN5020-01791 IN5020-04633 IN5020-04533 4.0 x 75 - IN5020-01790 IN5020-04632 IN5020-04532 4.0 x 50 - IN5020-04433 IN5020-04631 IN5020-04531 4.0 x 33 - IN5020-04432 IN5020-04646 IN5020-01732 4.6 x 250 - IN5020-04431 IN5020-04645 IN5020-01731 4.6 x 150 - IN5020-01772 IN5020-04647 IN5020-01768 4.6 x 125 - IN5020-01771 IN5020-04644 IN5020-01765 4.6 x 100 - IN5020-01776 IN5020-04643 IN5020-01764 4.6 x 75 - IN5020-01775 IN5020-04642 IN5020-01763 4.6 x 50 - IN5020-01770 IN5020-04641 IN5020-04541 4.6 x 33 IN5020-01774 IN5020-04441 Inertsil ODS-3 Guard Columns Dimensions 2 μm 2 μm 3 μm 3 μm 4 μm 4 μm 5 μm 5 μm Column Replacement Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 1.0 x 10 + 2 cartridges + 2 cartridges + 2 cartridges 1.5, 2.1 1.5 x 10 + 2 cartridges 2.1, 3.0 2.1 x 10 3.0 x 10 - - IN5020-19255 IN5020-19205 IN5020-19254 IN5020-19204 IN5020-19253 IN5020-19203 3.0 4.0 x 10 4.0, 4.6 3.0 x 20 IN5020-20362 IN5020-20311 IN5020-19355 IN5020-19305 IN5020-19354 IN5020-19304 IN5020-19353 IN5020-19303 2.1, 3.0 4.0 x 20 4.0, 4.6 IN5020-20379 IN5020-20328 - - -- -- IN5020-20396 IN5020-20345 IN5020-19155 IN5020-19105 IN5020-19154 IN5020-19104 IN5020-19153 IN5020-19103 - - IN5020-19055 IN5020-19005 IN5020-19054 IN5020-19004 IN5020-19053 IN5020-19003 - - IN5020-19555 IN5020-19505 IN5020-19554 IN5020-19504 IN5020-19553 IN5020-19503 - - IN5020-19455 IN5020-19405 IN5020-19454 IN5020-19404 IN5020-19453 IN5020-19403 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-35 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® ODS-P Colonnes HPLC/UHPLC Physical Properties Silica : 3 Series High Purity Silica Gel Particle Size : 3 μm, 5 μm Surface Area : 450 m2/g Pore Size : 100 Å Pore Volume : 1.05 mL/g Bonded Phase : Octadecyl Groups End-capping : None InertSearchTM for LC Carbon Loading : 29% : L1 Inertsil ® Applications USP Code pH Range : 2-7.5 Analysis of Polycyclic aromatic hydrocarbons (PAHs) Using FL Detector Inertsil ODS-P polymeric typDeaCt1a8Ncoo. lLuAm7n31d-e0l8iv7e1rs complete baseline separation of structurally similar compounds such as vitamins D2 and D3. Inertsil ODS-P columns are also ideal for the HPLC analysis of 17 PAH compounds, listed as target pollutants by the U.S. EPA. Analysis of Polycyclic aromatic hydrocarbons (PAHs) Using FL Detector 6 2 13 34 9 10 12 14 15 11 78 15 16 17 0 2 4 6 8 10 12 14 Time (min) Conditions Analyte: 1. Naphthalene System : GL-7400 HPLC system 2. 1-Methylnaphthalene (10 μg/L) 3. Acenaphthene (10 μg/L) Column : Inertsil ODS-P HP (3μm, 100 x 3.0 mm I.D.) 4. Fluorene (10 μg/L) 5. Phenanthrene (10 μg/L) Column Cat. No. : IN5020-87036 6. Anthracene (10 μg/L) 7. Fluoranthene (10 μg/L) Eluent : A) CH3OH 8. Pyrene (10 μg/L) B) H2O 9. Triphenylene (10 μg/L) A/B = 80/20 - 2 min- 80/20 – 3 min – 100/0, v/v 10. Benzo-[a]-anthracene (10 μg/L) 11. Chrysene (10 μg/L) Flow rate : 0.6 mL/min 12. Benzo-[b]-fluoranthene (10 μg/L) 13. Benzo-[k]-fluoranthene (10 μg/L) Col. Temp. : 40 ℃ 14. Benzo-[a]-pyrene (10 μg/L) 15. DiBenz-[a,h]-anthracene (10 μg/L) Detection : FL with time program (GL-7453A FL Detector) 16. Benzo-[g,h,i]-perylene (10 μg/L) 17. Indeno-[1,2,3-cd]pyrene (10 μg/L) Time (min) Ex. (nm) Em.(nm) (10 μg/L) 0.0 285 325 4.0 296 367 4.7 250 400 5.7 358 435 6.4 320 391 7.0 258 355 7.7 280 380 8.8 295 420 11.2 300 400 13.1 300 500 Injection Vol. : 5 μL Sample : PAHs LC LA731 Inertsil ODS-P HP N Naphthalene ナ ナフタレン Naphthalene ナフタレン 91-20-3 LC LA731 Inertsil ODS-P HP M Methylnaphthalene 1 マ メチルナフタレン1 1-Methylnaphthalene 1-メチルナフタレン 90-12-0 Reference LA337, LA607LC LA731 Inertsil ODS-P HP A Acenaphthene ア アセナフテン Acenaphtheneアセナフテン83-32-9 LC LA731 Inertsil ODS-P HP F Fluorene ハ フルオレン Fluorene フルオレン 86-73-7 E-36 LC LA731 Inertsil ODS-P HP P Phenanthrene ハ フェナントレン Phenanthrene フェナントレン 85-01-8 1 888-226-2775 :: [email protected] :: www.lifescience.ca LC LA731 Inertsil ODS-P HP A Anthracene ア アントラセン Anthracene アントラセン 120-12-7 CANADIANLC LA731 Inertsil ODS-P HP F Fluoranthene ハ フルオランテン Fluoranthene フルオランテン 206-44-0 LC LA731 Inertsil ODS-P HP P Pyrene ハ ピレン Pyrene ピレン 129-00-0 LC LA731 Inertsil ODS-P HP T Triphenylene タ トリフェニレン Triphenylene Benzo[ｌ]phenanthrene Isochrysene トリフェニレン ベンゾ[ｌ]フェナンスレン イソクリセン 217-59-4 LC LA731 Inertsil ODS-P HP B Benzo［a］anthracene ハ ベンゾ[a]アントラセン Benzo［a］anthracene ベンゾ[a]アントラセン 56-55-3 LC LA731 Inertsil ODS-P HP B Benzo[a]phenanthrene ハ ベンゾ[a]フェナントレン Benzo[a]phenanthrene Chrysene ベンゾ[a]フェナントレン クリセン 218-01-9 LIFE SCIENCELC LA731 Inertsil ODS-P HP B Benzo［b］fluoranthene ハ ベンゾ[b]フルオランテン Benzo［b］fluoranthene ベンゾ[b]フルオランテン 205-99-2 LC LA731 Inertsil ODS-P HP B Benzo[k]fluoranthene ハ ベンゾ[k]フルオランテン Benzo[k]fluoranthene ベンゾ[k]フルオランテン 207-08-9 LC LA731 Inertsil ODS-P HP B Benzo［a］pyrene ハ ベンゾ[a]ピレン \"Benzo［a］pyrene (3,4-Benzopyrene)\" ベンゾ[a]ピレン \"3,4-ベンゾピレン\" 50-32-8 LC LA731 Inertsil ODS-P HP D \"Dibenz［a,h］anthracene\" サ \"ジベンゾ［a,h］アントラセン\" \"Dibenz［a,h］anthracene\" \"Naphtho[1',2',2,3]phenanthrene\" \"ジベンゾ［a,h］アントラセン\" \"ナフト[1',2',2,3]ナフトフェナントレン\" 53-70-3 LC LA731 Inertsil ODS-P HP B \"Benzo[g,h,i]perylene\" ハ \"ベンゾ[g,h,i]ペリレン\" \"Benzo[g,h,i]perylene\" \"ベンゾ[g,h,i]ペリレン\" 191-24-2 LC LA731 Inertsil ODS-P HP I \"Indeno[1,2,3-cd]pyrene\" ア \"インデノ[1,2,3-cd]ピレン\" \"Indeno[1,2,3-cd]pyrene\" \"インデノ[1,2,3-cd]ピレン\" 193-39-5 LA731 0871 GL Sciences Inc. Cas. No. HPLC Liquid Chromatography Chromatogram C18 ODS OctaDecylSilyl polycyclic aromatic LA337 LA607 PAH arene polymeric column \"oil, coal, tar\" 高速液体クロマトグラフィー HPLCカラム ジーエルサイエンス株式会社 イナートサーチ イナートシル クロマトグラム 多環芳香族化合物 多核芳香族炭化水素 オクタデシル基 オクタ デシルシリル基 アレーン

Dimensions Inertsil ODS-P 5 μm HPLC/UHPLC Columns HPLC/UHPLC Columns 3 μm (200 bar) ID x Length (in mm) (200 bar) Inertsil® ODS-P IN5020-84716 1.0 x 250 IN5020-84736 IN5020-84715 Guard Column E (200 bar) 1.0 x 150 IN5020-84735 IN5020-84714 1.0 x 100 IN5020-84734 IN5020-84713 Reusable holder with universal self adjusting 1.0 x 75 IN5020-84733 IN5020-84712 connections and cartridges containing the same 1.0 x 50 IN5020-84732 IN5020-84711 1.0 x 33 IN5020-84731 IN5020-04716 high performance packing material 2.1 x 250 IN5020-04666 IN5020-04715 2.1 x 150 IN5020-04665 IN5020-04714 Did you know? 2.1 x 100 IN5020-04664 IN5020-04713 2.1 x 75 IN5020-04663 IN5020-04712 We also sell a wide selection of 2.1 x 50 IN5020-04662 IN5020-04711 solid phase extraction, syringe filters, 2.1 x 33 IN5020-04661 IN5020-04726 3.0 x 250 IN5020-04676 IN5020-04725 flash cartridges and a lot more. 3.0 x 150 IN5020-04675 IN5020-04724 3.0 x 100 IN5020-04674 IN5020-04723 For more information please 3.0 x 75 IN5020-04673 IN5020-04722 ask your Sales Representative today. 3.0 x 50 IN5020-04672 IN5020-04721 3.0 x 33 IN5020-04671 IN5020-04736 NEXT DAY DELIVERY! 4.0 x 250 IN5020-04686 IN5020-04735 4.0 x 150 IN5020-04685 IN5020-04734 4.0 x 100 IN5020-04684 IN5020-04733 4.0 x 75 IN5020-04683 IN5020-04732 4.0 x 50 IN5020-04682 IN5020-04731 4.0 x 33 IN5020-04681 IN5020-02002 4.6 x 250 IN5020-04696 IN5020-02001 4.6 x 150 IN5020-04695 IN5020-04744 4.6 x 100 IN5020-04694 IN5020-04743 IN5020-04693 IN5020-04742 4.6 x 75 IN5020-04692 IN5020-04741 IN5020-04691 4.6 x 50 4.6 x 33 Inertsil ODS-P Guard Columns Dimensions 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement ID (mm) ID x Length (mm) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 IN5020-19208 1.0 x 10 IN5020-19259 IN5020-19209 IN5020-19258 IN5020-19308 3.0 1.5 x 10 IN5020-19359 IN5020-19108 4.0, 4.6 3.0 x 10 IN5020-19159 IN5020-19309 IN5020-19358 IN5020-19008 2.1, 3.0 4.0 x 10 IN5020-19059 IN5020-19508 4.0, 4.6 3.0 x 20 IN5020-19559 IN5020-19109 IN5020-19158 IN5020-19408 4.0 x 20 IN5020-19459 IN5020-19009 IN5020-19058 IN5020-19509 IN5020-19558 IN5020-19409 IN5020-19458 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-37 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® C8-3 Colonnes HPLC/UHPLC Physical Properties Change in First Choice Column Retentivity Silica : 3 Series High Purity Silica Gel ODS-3 Particle Size : 2 μm, 3 μm, 5 μm, 10 μm Change in Selectivity Surface Area : 450 m2/g C8-3 Ph-3 Pore Size : 100 Å Change in Selectivity Pore Volume : 1.05 mL/g Bonded Phase : Octyl Groups End-capping : Yes Carbon Loading : 9% USP Code : L7 pH Range : 2-7.5 The same base silica gel and bonding technology that is used for Inertsil ODS-3 is also employed for Inertsil C8-3. The difference between the two phases is just the length of the hydrocarbon ligands. 2 to 10 μm particle size columns are also available to meet the requirement of various pharmacopeia methods. We recommend InertSustain C8 columns for all new method development. Comparison of Retentivity between Inertsil® ODS-3 and Inertsil® C8-3 4 4 Conditions 1 Column Size : 5 μm, 150 × 4.6 mm I.D. 2 35 Eluent : A) CH3CN B) H2O A/B = 70/30, v/v 1 Flow Rate : 1.0 mL/min Col. Temp. : 40 °C Detection : UV 254 nm Sample : 1. Naphthalene 2. Ethylbenzene 2 3. n-Propylbenzene 3 4. Anthracene 5 5. n-Butylbenzene 0 2 4 6 8 10 12 0 2 4 6 8 10 12 Time(min) Time(min) Inertsil C8-3 Inertsil ODS-3 Analysis of Losartan Potassium and Hydrochlorothiazide Tablets, Dissolution Test Conditions (Based on the Condition of United States Pharmacopeia 36-NF31) Column : Inertsil C8-3 (10 μm, 250 × 4.6 mm I.D.) 2 Eluent : A) CH3CN 1 B) 10 mM KH2PO4 (pH 2.5, H3PO4) A/B = 40/60, v/v Flow Rate : 2.3 mL/min Col. Temp. : 35 °C Detection : UV 230 nm Injection Vol. : 20 μL Sample : 1. Hydrochlorothiazide (14 mg/L) 2. Losartan potassium (60 mg/L) 0 2 46 8 10 Time (min) E-38 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN LIFE SCIENCE

HPLC/UHPLC Columns Dimensions 2 μm Inertsil C8-3 3 μm 5 μm Inertsil® C8-3 HPLC/UHPLC Columns (800 bar) 3 μm HP Series (200 bar) (200 bar) ID x Length (in mm) Guard Column E (200 bar) - (500 bar) - IN5020-84916 1.0 x 250 - IN5020-13522 IN5020-13512 Reusable holder with universal self adjusting 1.0 x 150 - - IN5020-84814 IN5020-84914 connections and cartridges containing the same 1.0 x 100 - - IN5020-84813 IN5020-84913 1.0 x 75 - - IN5020-84812 IN5020-84912 high performance packing material 1.0 x 50 - - IN5020-84811 IN5020-84911 1.0 x 33 - - IN5020-04816 IN5020-04916 UHPLC Guard Column (800 bar) 2.1 x 250 IN5020-84934 - IN5020-04815 IN5020-04915 2.1 x 150 - IN5020-14033 IN5020-04817 IN5020-04917 Dedicated Holder 2.1 x 125 IN5020-84933 IN5020-14032 IN5020-04814 IN5020-04914 2.1 x 100 IN5020-84932 - IN5020-04813 IN5020-04913 PEEK Guide Packing 2.1 x 75 IN5020-84931 IN5020-14031 IN5020-04812 IN5020-04912 2.1 x 50 - IN5020-14103 IN5020-04811 IN5020-04911 Tubing 2.1 x 33 IN5020-84930 IN5020-14102 2.1 x 30 - - - - Replacement Cartridges 3.0 x 250 IN5020-84939 IN5020-14101 IN5020-04826 IN5020-04926 3.0 x 150 - IN5020-14036 IN5020-04825 IN5020-04925 Simple to use cartridge system which includes 3.0 x 125 IN5020-84938 IN5020-14035 IN5020-04827 IN5020-04927 a tubing installed to the holder offering 3.0 x 100 IN5020-84937 - IN5020-04824 IN5020-04924 minimized dead volume. 3.0 x 75 IN5020-84936 IN5020-14034 IN5020-04823 IN5020-04923 3.0 x 50 - IN5020-14106 IN5020-04822 IN5020-04922 3.0 x 33 IN5020-84935 IN5020-14105 IN5020-04821 IN5020-04921 3.0 x 30 - - 4.0 x 250 - IN5020-14104 - - 4.0 x 150 - - IN5020-04836 IN5020-01903 4.0 x 125 - - IN5020-04835 IN5020-01902 4.0 x 100 - - IN5020-04837 IN5020-04935 4.0 x 75 - - IN5020-01913 IN5020-04934 4.0 x 50 - - IN5020-04833 IN5020-04933 4.0 x 33 - - IN5020-04832 IN5020-04932 4.6 x 250 - - IN5020-04831 IN5020-04931 4.6 x 150 - IN5020-14039 IN5020-01912 IN5020-01901 4.6 x 125 - IN5020-14038 IN5020-01911 IN5020-01900 4.6 x 100 - - IN5020-04845 IN5020-04945 4.6 x 75 - IN5020-14037 IN5020-04844 IN5020-04944 4.6 x 50 - IN5020-14109 IN5020-01910 IN5020-04943 4.6 x 33 - IN5020-14108 IN5020-04842 IN5020-04942 4.6 x 30 - IN5020-04841 IN5020-04941 IN5020-14107 - - Inertsil C8-3 Guard Columns Dimensions 2 μm 2 μm 3 μm HP 3 μm HP 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (UHPLC Guard Set) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 + 2 cartridges + 2 cartridges 2.1, 3.0 1.0 x 10 -- - - IN5020-19265 IN5020-19215 IN5020-19264 IN5020-19214 3.0 1.5 x 10 4.0, 4.6 2.1 x 10 IN5020-20363 IN5020-20312 IN5020-20354 IN5020-20303 IN5020-19365 IN5020-19315 IN5020-19364 IN5020-19314 2.1, 3.0 3.0 x 10 4.0, 4.6 4.0 x 10 IN5020-20380 IN5020-20329 IN5020-20371 IN5020-20320 - - - - 3.0 x 20 4.0 x 20 IN5020-20397 IN5020-20346 IN5020-20388 IN5020-20337 IN5020-19165 IN5020-19115 IN5020-19164 IN5020-19114 -- - - IN5020-19065 IN5020-19015 IN5020-19064 IN5020-19014 -- - - IN5020-19565 IN5020-19515 IN5020-19564 IN5020-19514 -- - - IN5020-19465 IN5020-19415 IN5020-19464 IN5020-19414 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-39 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® Ph-3 Colonnes HPLC/UHPLC Physical Properties Change in First Choice Column Retentivity Silica : 3 Series High Purity Silica Gel ODS-3 Particle Size : 2 μm, 3 μm, 5 μm Change in Selectivity Surface Area : 450 m2/g C8-3 Ph-3 Pore Size : 100 Å Change in Selectivity Pore Volume : 1.05 mL/g Bonded Phase : Phenyl Groups End-capping : None Carbon Loading : 9.5 % USP Code : L11 pH Range : 2-7.5 Just like InertSustain Phenyl, Inertsil Ph-3 has phenyl groups directly bonded to the silica gel which provides pure reverse phase characteristics that are critical to resolving highly polar compounds like acidic and basic pharmaceuticals. The near perfect phenyl phase coverage on this material results in symmetric, narrow peaks for even the most polar compounds while using simple eluents like aqueous acetonitrile or methanol. Comparison of Selectivity with Inertsil® ODS-3 2 1,2 Conditions Column Size : 5 μm, 150 × 4.6 mm I.D. Eluent : A) CH3OH B) H2O A/B = 90/10, v/v (ODS-3) A/B = 70/30, v/v (Ph-3) Flow Rate : 1.0 mL/min Col. Temp. : 30 °C Detection : UV 254 nm 1 1. Fluorene 0 2 4 6 8 10 0 2 4 6 8 10 Time(min) Time(min) Inertsil Ph-3 Inertsil ODS-3 2. Phenanthrene Analysis of Aromatic Hydrocarbons with various Bonded Phases Conditions Column : 5 μm, 150 × 4.6 mm I.D. 5 4,5 5 Eluent : A) CH3CN 1,2 2 B) H2O 3 A/B = 50/50, v/v (Phenyl columns) 2 A) CH3CN B) H2O 1 A/B = 70/50, v/v (ODS) 2 Flow Rate : 1 mL/min Col. Temp. : 40 °C Detection : UV 254 nm 4 1 4 3 C2H5 2. Naphthalene (CH2)2 CH3 1. Ethylbenzene 5. Anthracene 3. n-Propyl benzene 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 (CH2)3 CH3 Time(min) Time(min) Time(min) 4. n-Butylbenzene Inertsil Ph-3 Inertsil Ph Inertsil ODS-3 (Phenyl Group) (Alkyl Phenyl Group) (Alkyl Group) E-40 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Dimensions 2 μm Inertsil Ph-3 3 μm 5 μm Inertsil® Ph-3 HPLC/UHPLC Columns (800 bar) 3 μm HP Series (200 bar) (200 bar) ID x Length (in mm) Guard Column E (200 bar) - (500 bar) IN5020-13622 IN5020-85116 1.0 x 250 - IN5020-85014 IN5020-13612 Reusable holder with universal self adjusting 1.0 x 150 - - IN5020-85013 IN5020-85114 connections and cartridges containing the same 1.0 x 100 - - IN5020-85012 IN5020-85113 1.0 x 75 - - IN5020-85011 IN5020-85112 high performance packing material 1.0 x 50 - - IN5020-05016 IN5020-85111 1.0 x 33 - - IN5020-05015 IN5020-05116 UHPLC Guard Column (800 bar) 2.1 x 250 IN5020-85134 - IN5020-05014 IN5020-05115 2.1 x 150 IN5020-85133 IN5020-14043 IN5020-05013 IN5020-05114 Dedicated Holder 2.1 x 100 IN5020-85132 IN5020-14042 IN5020-05012 IN5020-05113 2.1 x 75 IN5020-85131 IN5020-14041 IN5020-05011 IN5020-05112 PEEK Guide Packing 2.1 x 50 - IN5020-14113 IN5020-05111 2.1 x 33 IN5020-85130 IN5020-14112 - Tubing 2.1 x 30 - - IN5020-05026 - 3.0 x 250 IN5020-85139 IN5020-14111 IN5020-05025 IN5020-05126 Replacement Cartridges 3.0 x 150 - IN5020-14046 IN5020-05125 3.0 x 125 IN5020-85138 IN5020-14045 - Simple to use cartridge system which includes 3.0 x 100 IN5020-85137 - IN5020-05024 - a tubing installed to the holder offering 3.0 x 75 IN5020-85136 IN5020-14044 IN5020-05023 IN5020-05124 minimized dead volume. 3.0 x 50 - IN5020-14116 IN5020-05022 IN5020-05123 3.0 x 33 IN5020-85135 IN5020-14115 IN5020-05021 IN5020-05122 3.0 x 30 - - IN5020-05121 4.0 x 250 - IN5020-14114 - 4.0 x 150 - - IN5020-05036 - 4.0 x 100 - - IN5020-05035 IN5020-01923 4.0 x 75 - - IN5020-01933 IN5020-01922 4.0 x 50 - - IN5020-05033 IN5020-05134 4.0 x 33 - - IN5020-05032 IN5020-05133 4.6 x 250 - - IN5020-05031 IN5020-05132 4.6 x 150 - IN5020-14049 IN5020-01932 IN5020-05131 4.6 x 100 - IN5020-14048 IN5020-01931 IN5020-01921 4.6 x 75 - IN5020-14047 IN5020-05044 IN5020-01920 4.6 x 50 - IN5020-14119 IN5020-01930 IN5020-05144 4.6 x 33 - IN5020-14118 IN5020-05042 IN5020-05143 4.6 x 30 - IN5020-05041 IN5020-05142 IN5020-14117 IN5020-05141 - - Inertsil Ph-3 Guard Columns Dimensions 2 μm 2 μm 3 μm HP 3 μm HP 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement Replacement Replacement ID (mm) ID x Length (mm) (UHPLC Guard Set) (UHPLC Guard Set) (Guard Column (Guard Column *Includes 1 holder Cartridges *Includes 1 holder Cartridges E Set) Cartridges E Set) Cartridges 1.0 1.5, 2.1 + 2 cartridges + 2 cartridges *Includes 1 holder *Includes 1 holder 2.1, 3.0 + 2 cartridges + 2 cartridges 3.0 1.0 x 10 - - - - IN5020-19267 IN5020-19217 IN5020-19266 IN5020-19216 4.0, 4.6 1.5 x 10 2.1, 3.0 2.1 x 10 IN5020-20364 IN5020-20313 IN5020-20355 IN5020-20304 IN5020-19367 IN5020-19317 IN5020-19366 IN5020-19316 4.0, 4.6 3.0 x 10 4.0 x 10 IN5020-20381 IN5020-20330 IN5020-20372 IN5020-20321 - - - - 3.0 x 20 4.0 x 20 IN5020-20398 IN5020-20347 IN5020-20389 IN5020-20338 IN5020-19167 IN5020-19117 IN5020-19166 IN5020-19116 - - - - IN5020-19067 IN5020-19017 IN5020-19066 IN5020-19016 - - - - IN5020-19567 IN5020-19517 IN5020-19566 IN5020-19516 - - - - IN5020-19467 IN5020-19417 IN5020-19466 IN5020-19416 UHPLC Guard Holder, 10 mm length (IN5020-08630), HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-41 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® CN-3 Colonnes HPLC/UHPLC Physical Properties 2.0 4) n-Butylbenzene Alkylbenzene 1.5 2) Naphthalene Polyaromatic Silica : 3 Series High Purity Silica Gel Relative Retention 5) Anthracene Particle Size : 3 μm, 5 μm Surface Area : 450 m2/g 1.0 Pore Size : 100 Å (10 nm) 0.5 Pore Volume : 1.05 mL/g Inertsil ODS-EP Inertsil Ph-3 Inertsil CN-3 Relative Retention to n-Propylbenzene Bonded Phase : Cyanopropryl G5roups 5 End-capping : None Carbon Loading : 14%2 Conditions USP Code : L110 52 Column Size : 5 μm, 150 × 4.6 mm I.D. 1 Eluent : A) CH3CN pH Range : 2-7.5 3 4 2 3 B) H2O 1 4 A/B = 70/30, v/v (ODS-EP) A/B = 40/60, v/v (Ph-3, CN-3) Inertsil CN-3 is a cyanopropyl bonded phase that 3 dipo4le and dipole interactions offered by the triple Flow Rate : 1.0 mL/min Due to provides boDCneotld.eTcetiinmonpc. y::a4Un0Vo°2C5g4rnomups. its chemistry, separation can be achieved for those compounds that could not be separated on a straight-chain-Saalmkpylel colu: 21m.. ENntahpsyhlbstheuanlzceenhneeas C8 or C18 bonded0 ph2ase4s. A6ltho8 ug10h In12ert14sil1O6 DS-E0P and In1e0 rtsil Ph2-03 has th30e abili0ty t2o re4cog6niz8e t1h0e e12lec1t4ro1n6-state, each colum34n.. nn--dBPeurotlypilyvblebenerznseznedeneifferent elution pattern. InertsiTlimCeN(m-in3) column can be tuhseedcofolurImnbenoTrtimtsfhieol(mPr12ri..hen50r)-ev3eversreseddpphhasaeseanapdpnliocramtiIoanenlTrpistms,heil(fmaCuisn425Nl)el)))y-NnA3-snaBetpehuhrqttyaphlcbuaaeeleninrnelziaeebnterioatneAPsolk.tlyyhlTabreohenmzeacetniocce olulummnnbiesfoo5.rrAeingtuihnrsaaceel.lnye shipped in a mixture of n-hexaInneer/tseiltOhDaSn-oElP. When using Relative Retention 1.0 Comparison of Recognition of Electronic-State between0.5various Inertsil® columns Inertsil ODS-EP Inertsil Ph-3 Inertsil CN-3 Relative Retention to n-Propylbenzene 55 2 Conditions 1 5 2 Column Size : 5 μm, 150 × 4.6 mm I.D. 3 4 1 Eluent : A) CH3CN 3 2 4 B) H2O 1 4 A/B = 70/30, v/v (ODS-EP) 3 A/B = 40/60, v/v (Ph-3, CN-3) Flow Rate : 1.0 mL/min Col. Temp. : 40 °C Detection : UV 254 nm Sample : 1. Ethylbenzene 2. Naphthalene 3. n-Propylbenzene 0 2 4 6 8 10 12 14 16 0 10 20 30 0 2 4 6 8 10 12 14 16 4. n-Butylbenzene Time(min) Time(min) Time(min) 5. Anthracene Inertsil ODS-EP Inertsil Ph-3 Inertsil CN-3 Comparison of Recognition of Electronic-State with Inertsil® ODS-3 13 1,2 3 Conditions 2 4 Column Size : 5 μm, 250 × 4.6 mm I.D. Eluent : 20 mM KH2PO4(pH 4.0, H3PO4) Flow Rate : 1.0 mL/min 4 Col. Temp. : 40 °C Detection : UV 210 nm 0 2 4 6 8 10 0 2 4 6 8 10 COOH COOH H COOH H H Time(min) Time(min) H2N C H H2N C H C C C C Inertsil CN-3 Inertsil ODS-3 CH2 CH2 HOOC H HOOC COOH CONH2 COOH 3. Fumaric acid 4. Maleic acid 1. Asparagine 2. Aspartic acid E-42 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Dimensions Inertsil CN-3 5 μm HPLC/UHPLC Columns HPLC/UHPLC Columns 3 μm (200 bar) ID x Length (in mm) (200 bar) Inertsil® CN-3 IN5020-85316 1.0 x 250 IN5020-85336 IN5020-13712 Guard Column E (200 bar) 1.0 x 150 IN5020-85335 IN5020-85314 1.0 x 100 IN5020-85334 IN5020-85313 Reusable holder with universal self adjusting 1.0 x 75 IN5020-85333 IN5020-85312 connections and cartridges containing the same 1.0 x 50 IN5020-85332 IN5020-85311 1.0 x 33 IN5020-85331 IN5020-05316 high performance packing material 2.1 x 250 IN5020-05266 IN5020-05315 2.1 x 150 IN5020-05265 IN5020-05314 Did you know? 2.1 x 100 IN5020-05264 IN5020-05313 We sell Soft Grip One-Piece 2.1 x 75 IN5020-05263 IN5020-05312 High Pressure Fingertight PEEK Fittings? 2.1 x 50 IN5020-05262 IN5020-05311 2.1 x 33 IN5020-05261 IN5020-05326 For more information please 3.0 x 250 IN5020-05276 IN5020-05325 ask your Sales Representative today. 3.0 x 150 IN5020-05275 IN5020-05324 3.0 x 100 IN5020-05274 IN5020-05323 3.0 x 75 IN5020-05273 IN5020-05322 3.0 x 50 IN5020-05272 IN5020-05321 3.0 x 33 IN5020-05271 IN5020-01943 4.0 x 250 IN5020-05286 IN5020-01942 4.0 x 150 IN5020-05285 IN5020-05334 4.0 x 100 IN5020-05284 IN5020-05333 4.0 x 75 IN5020-05283 IN5020-05332 4.0 x 50 IN5020-05282 IN5020-05331 4.0 x 33 IN5020-05281 IN5020-01941 4.6 x 250 IN5020-05296 IN5020-01940 4.6 x 150 IN5020-05295 IN5020-05344 4.6 x 100 IN5020-05294 IN5020-05343 IN5020-05293 IN5020-05342 4.6 x 75 IN5020-05292 IN5020-05341 IN5020-05291 4.6 x 50 4.6 x 33 Inertsil CN-3 Guard Columns Dimensions 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement ID (mm) ID x Length (mm) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges IN5020-19218 1.5, 2.1 1.0 x 10 IN5020-19269 IN5020-19219 IN5020-19268 3.0 1.5 x 10 4.0, 4.6 3.0 x 10 IN5020-19369 IN5020-19319 IN5020-19368 IN5020-19318 2.1, 3.0 4.0 x 10 4.0, 4.6 3.0 x 20 IN5020-19169 IN5020-19119 IN5020-19168 IN5020-19118 4.0 x 20 IN5020-19069 IN5020-19019 IN5020-19068 IN5020-19018 IN5020-19569 IN5020-19519 IN5020-19568 IN5020-19518 IN5020-19469 IN5020-19419 IN5020-19468 IN5020-19418 HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-43 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® NH2 Colonnes HPLC/UHPLC Physical Properties Silica : 3 Series High Purity Silica Gel Particle Size : 3 μm, 5 μm Surface Area : 450 m2/g Pore Size : 100 Å (10 nm) Pore Volume : 1.05 mL/g Bonded Phase : Aminopropyl Groups End-capping : None Carbon Loading : 8 % USP Code : L8 pH Range : 2 ~ 7.5 Inertsil NH2 column is chemically bonded with Aminopropyl groups. It is widely used for analyzing sugar in reversed phase mode. When compared to InertSustain NH2, the InertSustain NH2 provides a better performance on durability. However, compared to other brand aminopropyl columns, the Inertsil NH2 shows excellent and strong retentivity. Comparision of Aminopropyl columns 4 Conditions 23 1 Column Size : 5 μm, 250 × 4.6 mm I.D. 12 34 Eluent : A) CH3CN B) H2O A/B = 75/25, v/v Flow Rate : 1.0 mL/min Col. Temp. : 40 °C 0 10 20 0 2 4 6 8 10 12 14 Detection : RI Time(min) Time(min) E-44 Sample : 1. Fructose Inertsil NH2 InertSustain NH2 2. Glucose Selectivity of Inertsil® NH2 3. Sucrose 4. Maltose Conditions H3CO SIL-100A HO Diol Column : Inertsil Diol (5 μm, 150 × 3.0 mm I.D.) 1. Eugenol NH2 2. Pyridine CN-3 Eluent : A) n-Hexane/Ethanol = 100/1, v/v 2.5 5 7.5 10 12.5 15 17.5 20 22.5 B) Ethanol N Retention Time(min) A/B = 100/0 – 30 min – 25/75, v/v Flow Rate : 0.4 mL/min Col. Temp. : 40 °C NH2 3. Aniline OH 4. Benzyl alcohol Detection : UV 215 nm 6, 7 OH 5. Phenol OH 6. α-Naphthol 2 45 89 H 1 3 N 7. Carbazole N CH3 8. Caffeine NO N H3C N CH3 O 0 10 20 HO OH Time(min) 30 9. Resorcinol CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Dimensions Inertsil NH2 5 μm (200 bar) Inertsil® NH2 HPLC/UHPLC Columns 3 μm (200 bar) ID x Length (in mm) IN5020-85516 Guard Column E (200 bar) 1.0 x 250 IN5020-85536 IN5020-85515 1.0 x 150 IN5020-85535 Reusable holder with universal self adjusting 1.0 x 125 - connections and cartridges containing the same 1.0 x 100 - IN5020-85514 1.0 x 75 IN5020-85534 IN5020-85513 high performance packing material 1.0 x 50 IN5020-85533 IN5020-85512 1.0 x 33 IN5020-85532 IN5020-85511 Did you know? 2.1 x 250 IN5020-85531 IN5020-05516 2.1 x 150 IN5020-05466 IN5020-05515 We also sell Vials, Caps, 2.1 x 125 IN5020-05465 Inserts & 96-well plates. 2.1 x 100 - 2.1 x 75 - IN5020-05514 For more information please 2.1 x 50 IN5020-05464 IN5020-05513 ask your Sales Representative today. 2.1 x 33 IN5020-05463 IN5020-05512 3.0 x 250 IN5020-05462 IN5020-05511 DENLDIEVAXEYTRY! 3.0 x 150 IN5020-05461 IN5020-05526 3.0 x 125 IN5020-05476 IN5020-05525 3.0 x 100 IN5020-05475 3.0 x 75 - 3.0 x 50 - IN5020-05524 3.0 x 30 IN5020-05474 IN5020-05523 4.0 x 250 IN5020-05473 IN5020-05522 4.0 x 150 IN5020-05472 IN5020-05520 4.0 x 125 IN5020-05470 IN5020-05536 4.0 x 100 IN5020-05486 IN5020-05535 4.0 x 75 IN5020-05485 4.0 x 50 - 4.0 x 33 - IN5020-05534 4.6 x 250 IN5020-05484 IN5020-05533 4.6 x 150 IN5020-05483 IN5020-05532 4.6 x 125 IN5020-05482 IN5020-05531 4.6 x 100 IN5020-05481 IN5020-05546 4.6 x 75 IN5020-05496 IN5020-05545 4.6 x 50 IN5020-05495 4.6 x 33 - - IN5020-05544 IN5020-05494 IN5020-05543 IN5020-05493 IN5020-05542 IN5020-05492 IN5020-05541 IN5020-05491 Inertsil NH2 Guard Columns Dimensions 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement ID (mm) ID x Length (mm) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 1.0 x 10 IN5020-19271 IN5020-19221 IN5020-19270 IN5020-19220 3.0 1.5 x 10 IN5020-19371 4.0, 4.6 3.0 x 10 IN5020-19171 IN5020-19321 IN5020-19370 IN5020-19320 2.1, 3.0 4.0 x 10 IN5020-19071 4.0, 4.6 3.0 x 20 IN5020-19571 IN5020-19121 IN5020-19170 IN5020-19120 4.0 x 20 IN5020-19471 IN5020-19021 IN5020-19070 IN5020-19020 IN5020-19521 IN5020-19570 IN5020-19520 IN5020-19421 IN5020-19470 IN5020-19420 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-45 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® Diol Colonnes HPLC/UHPLC Physical Properties Silica : 3 Series High Purity Silica Gel Particle Size : 3 μm, 5 μm Surface Area : 450 m2/g Pore Size : 100 Å Pore Volume : 1.05 mL/g Bonded Phase : Diol Groups (Dihydroxypropyl Groups) End-capping : None Carbon Loading : 20% USP Code : L20 pH Range : 2-7.5 Inertsil Diol, dihydroxypropyl groups chemical bonded onto silica gel, can be used for water group SEC (GFC) or organic solvents SEC (GPC). As features of the packing material, it can analyze with several columns in series since 20 Mpa as the maximum operating pressure, and it is higher than polymer base columns. With coupling 2 columns of different pore size, in series, it can be used for broader range of molecular weight. General internal diameter of SEC columns are 7-8 mm. But even if it’s a 4.6 mm I.D. column, it can obtain calibration curve with smaller elution volume rather than the 7.6 mm I.D. column. Therefore it can be analyzed with saving solvent, environment conservation, and low cost. Comparison of selectivity between Diol column and Silica column 1 Conditions Column Size : 5 μm, 150 × 4.6 mm I.D. Eluent : A) n-Hexane B) Ethanol 24 A/B = 85/15, v/v 3 5 Flow Rate : 1.0 mL/min Col. Temp. : 40 °C Detection : UV 254 nm 0 10 Time(min) 20 Sample : O OH 1 O Inertsil Diol H HO H 2,3 4 5 O HH 1. Progesterone HH O 2. Corticosterone OH OH HO OH O O HO OH O OH OH OH H H 0 10 Time(min) 20 HH HH HH O O Inertsil SIL-100A O 3. Cortisone 4. Prednisone 5. Prednisolone Selectivity of Inertsil Diol Conditions H3CO SIL-100A HO Diol Column : Inertsil Diol (5 μm, 150 × 3.0 mm I.D.) 1. Eugenol NH2 2. Pyridine CN-3 Eluent : A) n-Hexane/Ethanol = 100/1, v/v 3. Aniline 4. Benzyl alcohol B) Ethanol N A/B = 100/0 – 30 min – 25/75, v/v Flow Rate : 0.4 mL/min Col. Temp. : 40 °C NH2 OH Detection : UV 215 nm 6, 7 OH 5. Phenol OH 6. α-Naphthol 2 89 H 45 N 7. Carbazole 3 1 N CH3 8. Caffeine NO N H3C N CH3 O 0 10 20 HO OH Time(min) 30 9. Resorcinol 2.5 5 7.5 10 12.5 15 17.5 20 22.5 Retention Time(min) E-46 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Dimensions Inertsil Diol 5 μm HPLC/UHPLC Columns HPLC/UHPLC Columns 3 μm (200 bar) ID x Length (in mm) (200 bar) Inertsil® Diol IN5020-86516 1.0 x 250 IN5020-86536 IN5020-86515 Guard Column E (200 bar) 1.0 x 150 IN5020-86535 IN5020-86514 1.0 x 100 IN5020-86534 IN5020-86513 Reusable holder with universal self adjusting 1.0 x 75 IN5020-86533 IN5020-86512 connections and cartridges containing the same 1.0 x 50 IN5020-86532 IN5020-86511 1.0 x 33 IN5020-86531 IN5020-05616 high performance packing material 2.1 x 250 IN5020-05416 IN5020-05615 2.1 x 150 IN5020-05415 IN5020-05614 Did you know? 2.1 x 100 IN5020-05414 IN5020-05613 2.1 x 75 IN5020-05413 IN5020-05612 We sell Gas Chromatography 2.1 x 50 IN5020-05412 IN5020-05611 Columns & Accessories? 2.1 x 33 IN5020-05411 IN5020-05626 3.0 x 250 IN5020-05426 IN5020-05625 For more information please 3.0 x 150 IN5020-05425 IN5020-05624 ask your Sales Representative today. 3.0 x 100 IN5020-05424 IN5020-05623 3.0 x 75 IN5020-05423 IN5020-05622 3.0 x 50 IN5020-05422 IN5020-05621 3.0 x 33 IN5020-05421 IN5020-05636 4.0 x 250 IN5020-05436 IN5020-05635 4.0 x 150 IN5020-05435 IN5020-05634 4.0 x 100 IN5020-05434 IN5020-05633 4.0 x 75 IN5020-05433 IN5020-05632 4.0 x 50 IN5020-05432 IN5020-05631 4.0 x 33 IN5020-05431 IN5020-05646 4.6 x 250 IN5020-05446 IN5020-05645 4.6 x 150 IN5020-05445 IN5020-05644 4.6 x 100 IN5020-05444 IN5020-05643 IN5020-05443 IN5020-05642 4.6 x 75 IN5020-05442 IN5020-05641 IN5020-05441 4.6 x 50 4.6 x 33 Inertsil Diol Guard Columns Dimensions 3 μm 3 μm 5 μm 5 µm Column Replacement Replacement ID (mm) ID x Length (mm) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 1.0 x 10 IN5020-19273 IN5020-19223 IN5020-19272 IN5020-19222 3.0 1.5 x 10 4.0, 4.6 3.0 x 10 IN5020-19373 IN5020-19323 IN5020-19372 IN5020-19322 2.1, 3.0 4.0 x 10 4.0, 4.6 3.0 x 20 IN5020-19173 IN5020-19123 IN5020-19172 IN5020-19122 4.0 x 20 IN5020-19073 IN5020-19023 IN5020-19072 IN5020-19022 IN5020-19573 IN5020-19523 IN5020-19572 IN5020-19522 IN5020-19473 IN5020-19423 IN5020-19472 IN5020-19422 HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-47 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® SIL-100A Colonnes HPLC/UHPLC Physical Properties Silica : 3 Series High Purity Silica Gel Particle Size : 3 μm, 5 μm Surface Area : 450 m2/g Pore Size : 100 Å (10 nm) Pore Volume : 1.05 mL/g Bonded Phase : None End-capping : None Carbon Loading : - % USP Code : L3 pH Range : 2-7.5 Inertsil SIL-100A is a pure silica gel column available in normal phase mode. Because of the high quality of its silica gel, Inertsil SIL-100A achieves separation with sharp peaks and provides high column-to-column reproducibility. This excellent silica gel ideally designed for HPLC is the basis for “Inertsil 3-series” of GL Sciences. GL Sciences is the first company which emphasized the importance of silica-gel purity and determined the nature of the silanol impurities in the silica gel. GL Sciences has established a successful manufacturing process for ultra pure silica gel with smooth and rigid surfaces. Particles of Inertsil SIL-100A stand out by the smooth surface, uniformity in size and spherical shape. SEM photos of Inertsil SIL-100A and other silica gels brand Inertsil SIL-100A Silica gel A Silica gel B Silica gel C Silica gel D Selectivity of InertsilTM SIL-100A Conditions H3CO 1. Eugenol SIL-100A Column : Inertsil SIL-100A (5 μm, 150 × 3.0 mm I.D.) HO 2. Pyridine Diol Eluent : A) n- Hexane/Ethanol = 100/1, v/v NH2 N CN-3 B) Ethanol A/B = 100/0 – 30 min – 25/75, v/v NH2 3. Aniline Flow Rate : 0.4 mL/min OH Col. Temp. : 40 °C 4. Benzyl alcohol Detection : UV 215 nm 6 2 OH 5. Phenol OH 6. α-Naphthol 3 H 7. Carbazole N 1 7 5 9 CH3 4 NO 8 N N N H3C CH3 8. Caffeine HO O 9. Resorcinol OH 0 10 20 30 Time(min) 2.5 5 7.5 10 12.5 15 17.5 20 22.5 CANADIAN Retention Time(min) E-48 LIFE SCIENCE 1 888-226-2775 :: [email protected] :: www.lifescience.ca

Dimensions Inertsil SIL-100A 5 μm HPLC/UHPLC Columns HPLC/UHPLC Columns 3 μm (200 bar) ID x Length (in mm) (200 bar) Inertsil® SIL-100A IN5020-84316 1.0 x 250 - IN5020-13412 Guard Column E (200 bar) 1.0 x 150 IN5020-13422 IN5020-84314 1.0 x 100 IN5020-84214 IN5020-84313 Reusable holder with universal self adjusting 1.0 x 75 IN5020-84213 IN5020-84312 connections and cartridges containing the same 1.0 x 50 IN5020-84212 IN5020-84311 1.0 x 33 IN5020-84211 IN5020-04316 high performance packing material 2.1 x 250 IN5020-04216 IN5020-04315 2.1 x 150 IN5020-04215 IN5020-04314 Did you know? 2.1 x 100 IN5020-04214 IN5020-04313 2.1 x 75 IN5020-04213 IN5020-04312 We sell a wide range of HPLC maintenance 2.1 x 50 IN5020-04212 IN5020-04311 products such as Pump Seals, 2.1 x 33 IN5020-04211 IN5020-04326 3.0 x 250 IN5020-04226 IN5020-04325 Check Valves, Piston Seals and Lamps. 3.0 x 150 IN5020-04225 IN5020-04324 3.0 x 100 IN5020-04224 IN5020-04323 For more information please 3.0 x 75 IN5020-04223 IN5020-04322 ask your Sales Representative today. 3.0 x 50 IN5020-04222 IN5020-04321 3.0 x 33 IN5020-04221 IN5020-04336 NEXT DAY DELIVERY! 4.0 x 250 IN5020-04236 IN5020-04335 4.0 x 150 IN5020-04235 IN5020-04334 4.0 x 100 IN5020-01703 IN5020-04333 4.0 x 75 IN5020-04233 IN5020-04332 4.0 x 50 IN5020-04232 IN5020-04331 4.0 x 33 IN5020-04231 IN5020-01712 4.6 x 250 IN5020-01702 IN5020-01711 4.6 x 150 IN5020-01701 IN5020-04344 4.6 x 100 IN5020-04244 IN5020-04343 IN5020-01700 IN5020-04342 4.6 x 75 IN5020-04242 IN5020-04341 IN5020-04241 4.6 x 50 4.6 x 33 Inertsil SIL-100A Guard Columns Dimensions 3 μm 3 µm 5 μm 5 µm Column Replacement Replacement ID (mm) ID x Length (mm) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 1.0 x 10 IN5020-19277 IN5020-19227 IN5020-19276 IN5020-19226 3.0 1.5 x 10 4.0, 4.6 3.0 x 10 IN5020-19377 IN5020-19327 IN5020-19376 IN5020-19326 2.1, 3.0 4.0 x 10 4.0, 4.6 3.0 x 20 IN5020-19177 IN5020-19127 IN5020-19176 IN5020-19126 4.0 x 20 IN5020-19077 IN5020-19027 IN5020-19076 IN5020-19026 IN5020-19577 IN5020-19527 IN5020-19576 IN5020-19526 IN5020-19477 IN5020-19427 IN5020-19476 IN5020-19426 HPLC Guard Holder, 10 mm length (IN5020-08500), HPLC Guard Holder, 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-49 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® ODS-HL (High Carbon Load) Inertsil ODS-HL Colonnes HPLC/UHPLC Dimensions 3 μm HP Series 3 μm 5 μm (500 bar) (200 bar) (200 bar) ID x Length (in mm) - IN5020-87271 IN5020-87147 1.0 x 250 IN5020-87146 1.0 x 150 - IN5020-87270 IN5020-87145 1.0 x 100 IN5020-87144 1.0 x 75 - IN5020-87269 IN5020-87143 1.0 x 50 IN5020-87142 Physical Properties 1.0 x 30 - IN5020-87268 IN5020-87108 2.1 x 250 IN5020-87107 Silica : 3 Series High Purity Silica Gel 2.1 x 150 - IN5020-87267 IN5020-87106 2.1 x 125 IN5020-87105 Particle Size : 3 μm, 5 μm 2.1 x 100 - IN5020-87266 IN5020-87104 2.1 x 75 IN5020-87103 Surface Area : 450 m2/g 2.1 x 50 IN5020-87320 IN5020-87232 IN5020-87102 2.1 x 30 IN5020-87116 Pore Size : 100 Å 3.0 x 250 IN5020-87319 IN5020-87231 IN5020-87115 3.0 x 150 IN5020-87114 Pore Volume : 1.05 mL/g 3.0 x 125 - IN5020-87230 IN5020-87113 3.0 x 100 IN5020-87112 Bonded Phase : Octadecyl Groups 3.0 x 75 IN5020-87318 IN5020-87229 IN5020-87111 3.0 x 50 IN5020-87110 End-capping : Yes 3.0 x 30 IN5020-87317 IN5020-87228 IN5020-87124 4.0 x 250 IN5020-87123 Carbon Loading : 23% 4.0 x 150 IN5020-87316 IN5020-87227 IN5020-87122 4.0 x 125 IN5020-87121 USP Code : L1 4.0 x 100 IN5020-87315 IN5020-87226 IN5020-87120 4.0 x 75 IN5020-87119 pH Range : 2-7.5 4.0 x 50 IN5020-87326 IN5020-87240 IN5020-87118 4.0 x 30 IN5020-87132 4.6 x 250 IN5020-87325 IN5020-87239 IN5020-87131 4.6 x 150 IN5020-87130 Ultra high retentivity, high-density bonding of C18 phase. Enable 4.6 x 125 - IN5020-87238 IN5020-87129 to recognize even slight isomer differences as o-, m-, p-structure. 4.6 x 100 IN5020-87128 Highly inert packing material: Pure hydrophobic interaction 4.6 x 75 IN5020-87324 IN5020-87237 IN5020-87127 without generating any secondary interaction. 4.6 x 50 IN5020-87126 4.6 x 30 IN5020-87323 IN5020-87236 Column IN5020-87322 IN5020-87235 ID (mm) IN5020-87321 IN5020-87234 1.0 1.5, 2.1 - IN5020-87248 2.1, 3.0 4.0, 4.6 - IN5020-87247 3.0 - IN5020-87246 4.0, 4.6 Guard Column E (200 bar) - IN5020-87245 Reusable holder with universal - IN5020-87244 self adjusting connections and cartridges containing the same - IN5020-87243 high performance packing material - IN5020-87242 IN5020-87332 IN5020-87256 IN5020-87331 IN5020-87255 - IN5020-87254 IN5020-87330 IN5020-87253 IN5020-87329 IN5020-87252 IN5020-87328 IN5020-87251 IN5020-87327 IN5020-87250 Inertsil ODS-HL Guard Columns Dimensions 3 μm 3 μm 5 μm 5 μm Replacement Replacement ID x Length (mm) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges + 2 cartridges + 2 cartridges IN5020-87209 1.0 x 10 IN5020-87306 IN5020-87305 IN5020-87210 IN5020-87211 1.5 x 10 IN5020-87308 IN5020-87207 3.0 x 10 IN5020-87304 IN5020-87307 IN5020-87212 IN5020-87205 4.0 x 10 IN5020-87302 IN5020-87215 3.0 x 20 IN5020-87312 IN5020-87303 IN5020-87208 IN5020-87213 4.0 x 20 IN5020-87310 IN5020-87301 IN5020-87206 IN5020-87311 IN5020-87216 IN5020-87309 IN5020-87214 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) E-50 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Inertsil® SIL-150A Physical Properties HPLC/UHPLC Columns Silica : 2 Series High Purity Silica Gel Particle Size : 5 μm Surface Area : 320 m2/g Pore Size : 150 Å Pore Volume : 1.20 mL/g Bonded Phase : None End-capping : None Carbon Loading : - % USP Code : L3 pH Range : 2-7.5 Inertsil SIL-150A is ultra pure silica gel column, and this ultra pure silica gel contains very low levels of metal impurities and is durable and free from dents and cracks which can cause premature column failure. Compared to Inertsil SIL-100A, the silica’s surface area is smaller (320 m²/g). Retentivity of Inertsil SIL-150A is weaker than that of Inertsil SIL-100A. Dimensions Inertsil SIL-150A Guard Column E (200 bar) 5 μm ID x Length (in mm) Reusable holder with universal (200 bar) self adjusting connections and 2.1 x 250 cartridges containing the same 2.1 x 150 IN5020-01025 high performance packing 3.0 x 250 IN5020-01021 material 3.0 x 150 IN5020-01026 4.0 x 250 IN5020-01022 4.0 x 150 IN5020-01027 4.6 x 250 IN5020-01023 4.6 x 150 IN5020-01028 IN5020-01024 Inertsil SIL-150A Guard Columns Dimensions 5 μm 5 μm Column Replacement ID (mm) ID x Length (mm) (Guard Column E Set) *Includes 1 holder Cartridges + 2 cartridges 3.0 x 10 IN5020-19189 IN5020-19139 2.1, 3.0 4.0 x 10 IN5020-19089 IN5020-19039 4.0, 4.6 3.0 x 20 IN5020-19589 IN5020-19539 2.1, 3.0 4.0 x 20 IN5020-19489 IN5020-19439 4.0, 4.6 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-51 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® HILIC Colonnes HPLC/UHPLC Physical Properties Silica : 3 Series High Purity Silica Gel Particle Size : 3 μm, 5 μm Surface Area : 450 m2/g Pore Size : 100 Å Pore Volume : 1.05 mL/g Bonded Phase : Diol Groups (Dihydroxypropyl Groups) End-capping : None Carbon Loading : 20% USP Code : L20 pH Range : 2-7.5 HILIC is the abbreviated name for Hydrophilic Interaction Chromatography. It was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when organic concentration is raised, it shows a strong retention. Moreover, it can get more stable analysis by adding a basic solvent to the eluate, such as ammonium acetate. Separation mode from Reverse phase to HILIC ODS column ODS column ODS column HILIC column HILIC column Reverse phase mode pH control Using Ion pair reagent Using higher organic solvent Add buffer No retention No retention No retention Improve to a stable condition 1. Allantoin Reproducibility of adjust eluent 1 1 1 1 1 first time 2nd time 3rd time 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 Time(min) Time(min) Time(min) Time(min) Time(min) Conditions Conditions Conditions Conditions Conditions Column : ODS Column : ODS Column : ODS Column : Inertsil HILIC Column : Inertsil HILIC Eluent : A) CH3CN Eluent : A) CH3CN Eluent : A) CH3CN Eluent : A) CH3CN Eluent : A) CH3CN B) H2O B) 10 mM phosphate buffer(pH 7.5) B) IPCC-04 B) H2O B) 10 mM CH3COONH4 A/B = 5/95, v/v A/B = 5/95, v/v A/B = 5/95, v/v A/B = 95/5, v/v A/B = 95/5, v/v E-52 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Dimensions Inertsil HILIC 5 μm HPLC/UHPLC Columns HPLC/UHPLC Columns 3 μm (200 bar) ID x Length (in mm) (200 bar) Inertsil® HILIC IN5020-86716 1.0 x 250 IN5020-86736 IN5020-86715 Guard Column E (200 bar) 1.0 x 150 IN5020-86735 IN5020-86714 1.0 x 100 IN5020-86734 IN5020-86713 Reusable holder with universal self adjusting 1.0 x 75 IN5020-86733 IN5020-86712 connections and cartridges containing the same 1.0 x 50 IN5020-86732 IN5020-86711 1.0 x 33 IN5020-86731 IN5020-07706 high performance packing material 2.1 x 250 IN5020-07766 IN5020-07705 2.1 x 150 IN5020-07765 IN5020-07704 Did you know? 2.1 x 100 IN5020-07764 IN5020-07703 Canadian Life Science is an 2.1 x 75 IN5020-07763 IN5020-07702 Authorized Distributor for 2.1 x 50 IN5020-07762 IN5020-07701 Honeywell Research Chemicals? 2.1 x 33 IN5020-07761 IN5020-07716 3.0 x 250 IN5020-07776 IN5020-07715 For more information please 3.0 x 150 IN5020-07775 IN5020-07714 ask your Sales Representative today. 3.0 x 100 IN5020-07774 IN5020-07713 3.0 x 75 IN5020-07773 IN5020-07712 3.0 x 50 IN5020-07772 IN5020-07711 3.0 x 33 IN5020-07771 IN5020-07726 4.0 x 250 IN5020-07786 IN5020-07725 4.0 x 150 IN5020-07785 IN5020-07724 4.0 x 100 IN5020-07784 IN5020-07723 4.0 x 75 IN5020-07783 IN5020-07722 4.0 x 50 IN5020-07782 IN5020-07721 4.0 x 33 IN5020-07781 IN5020-07736 4.6 x 250 IN5020-07796 IN5020-07735 4.6 x 150 IN5020-07795 IN5020-07734 4.6 x 100 IN5020-07794 IN5020-07733 4.6 x 75 IN5020-07793 IN5020-07732 4.6 x 50 IN5020-07792 IN5020-07731 4.6 x 33 IN5020-07791 Inertsil HILIC Guard Columns Dimensions 3 μm 3 μm 5 μm 5 μm Column Replacement Replacement ID (mm) ID x Length (mm) (Guard Column E Set) (Guard Column E Set) *Includes 1 holder Cartridges *Includes 1 holder Cartridges 1.0 + 2 cartridges + 2 cartridges 1.5, 2.1 1.0 x 10 IN5020-19275 IN5020-19225 IN5020-19274 IN5020-19224 3.0 1.5 x 10 IN5020-19375 4.0, 4.6 3.0 x 10 IN5020-19175 IN5020-19325 IN5020-19374 IN5020-19324 2.1, 3.0 4.0 x 10 IN5020-19075 4.0, 4.6 3.0 x 20 IN5020-19575 IN5020-19125 IN5020-19174 IN5020-19124 4.0 x 20 IN5020-19475 IN5020-19025 IN5020-19074 IN5020-19024 IN5020-19525 IN5020-19574 IN5020-19524 IN5020-19425 IN5020-19474 IN5020-19424 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-53 LIFE SCIENCE

Colonnes HPLC/UHPLC Inertsil® AX Colonnes HPLC/UHPLC Physical Properties Silica : 3 Series High Purity Silica Gel Particle Size : 5 μm Surface Area : 450 m2/g Pore Size : 100 Å Pore Volume : 1.05 mL/g Bonded Phase : Diethylaminopropyl Groups End-capping : None Inertsil AX Carbon Loading : 17% Dimensions 5 μm (200 bar) USP Code :- ID x Length (in mm) pH Range : 2-7.5 1.0 x 250 IN5020-80116 1.0 x 150 IN5020-80115 Inertsil AX has diethylamino groups bonded to silica gel by an alkyl chain. The diethylamino 1.0 x 100 IN5020-80114 groups offer anionic functions required for anion exchange chromatography. It is mainly used for analyses of acidic compounds. Conventional ion-exchange columns used to show 1.0 x 75 IN5020-80113 inconsistent results from lot to lot. However, Inertsil AX is manufactured under strict quality control in order to offer excellent lot-to-lot reproducibility. The retentivity of Inertsil AX 1.0 x 50 IN5020-80112 is influenced by the concentration of buffer. The retention time can be adjusted by the concentration of buffer. 1.0 x 33 IN5020-80111 2.1 x 250 IN5020-07216 2.1 x 150 IN5020-07215 2.1 x 100 IN5020-07214 2.1 x 75 IN5020-07213 2.1 x 50 IN5020-07212 Guard Column E (200 bar) 2.1 x 33 IN5020-07211 Reusable holder with universal 3.0 x 250 IN5020-07226 self adjusting connections and cartridges containing the same 3.0 x 150 IN5020-07225 high performance packing material 3.0 x 100 IN5020-07224 3.0 x 75 IN5020-07223 3.0 x 50 IN5020-07222 3.0 x 33 IN5020-07221 4.0 x 250 IN5020-07236 Inertsil AX Guard Columns 4.0 x 150 IN5020-07235 Dimensions 5 μm 5 μm Column 4.0 x 100 IN5020-07234 Replacement ID (mm) ID x Length (mm) (Guard Column E Set) 4.0 x 75 IN5020-07233 *Includes 1 holder Cartridges + 2 cartridges 4.0 x 50 IN5020-07232 1.0 x 10 IN5020-19283 IN5020-19233 1.0 4.0 x 33 IN5020-07231 1.5 x 10 IN5020-19383 IN5020-19333 1.5, 2.1 4.6 x 250 IN5020-07246 3.0 x 10 IN5020-19183 IN5020-19133 3.0 4.6 x 150 IN5020-07245 4.0 x 10 IN5020-19083 IN5020-19033 4.0, 4.6 4.6 x 100 IN5020-07244 3.0 x 20 IN5020-19583 IN5020-19533 2.1, 3.0 4.6 x 75 IN5020-07243 4.0 x 20 IN5020-19483 IN5020-19433 4.0, 4.6 4.6 x 50 IN5020-07242 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 4.6 x 33 IN5020-07241 E-54 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Inertsil® CX Physical Properties HPLC/UHPLC Columns Silica : 3 Series High Purity Silica Gel Particle Size : 5 μm Surface Area : 450 m2/g Pore Size : 100 Å Pore Volume : 1.05 mL/g Bonded Phase : Benzensulfonyl Groups End-capping : None Inertsil CX Carbon Loading : 14% Dimensions 5 μm (200 bar) USP Code : L9 ID x Length (in mm) pH Range : 2-7.5 1.0 x 250 IN5020-80016 1.0 x 150 IN5020-80015 Inertsil CX has benzenesulfonyl groups bonded to silica gel by an alkyl chain. The sulfonyl 1.0 x 100 IN5020-80014 groups at the end of the structure offer cationic functions required for the cation exchange chromatography. It is mainly used for analyses of basic compounds. Inertsil CX is manufactured 1.0 x 75 IN5020-80013 under strict quality control in order to offer excellent lot-to-lot reproducibility as the same as Inertsil AX. Inertsil CX has high ion exchange capacity and provides high retentivity and 1.0 x 50 IN5020-80012 selectivity. Therefore, it is also suited for analyzing amino acids and nucleobases. 1.0 x 33 IN5020-80011 2.1 x 250 IN5020-07116 2.1 x 150 IN5020-07115 2.1 x 100 IN5020-07114 2.1 x 75 IN5020-07113 2.1 x 50 IN5020-07112 Guard Column E (200 bar) 2.1 x 33 IN5020-07111 Reusable holder with universal 3.0 x 250 IN5020-07126 self adjusting connections and cartridges containing the same 3.0 x 150 IN5020-07125 high performance packing material 3.0 x 100 IN5020-07124 3.0 x 75 IN5020-07123 3.0 x 50 IN5020-07122 3.0 x 33 IN5020-07121 4.0 x 250 IN5020-07136 Inertsil CX Guard Columns 4.0 x 150 IN5020-07135 Dimensions 5 μm 5 μm Column 4.0 x 100 IN5020-07134 Replacement ID (mm) ID x Length (mm) (Guard Column E Set) 4.0 x 75 IN5020-07133 *Includes 1 holder Cartridges + 2 cartridges 4.0 x 50 IN5020-07132 1.0 x 10 IN5020-19284 IN5020-19234 1.0 4.0 x 33 IN5020-07131 1.5 x 10 IN5020-19384 IN5020-19334 1.5, 2.1 4.6 x 250 IN5020-07146 3.0 x 10 IN5020-19184 IN5020-19134 3.0 4.6 x 150 IN5020-07145 4.0 x 10 IN5020-19084 IN5020-19034 4.0, 4.6 4.6 x 100 IN5020-07144 3.0 x 20 IN5020-19584 IN5020-19534 2.1, 3.0 4.6 x 75 IN5020-07143 4.0 x 20 IN5020-19484 IN5020-19434 4.0, 4.6 4.6 x 50 IN5020-07142 Holder for 10 mm length (IN5020-08500), Holder for 20 mm length (IN5020-08550) 4.6 x 33 IN5020-07141 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN E-55 LIFE SCIENCE

Colonnes HPLC/UHPLCColonnes HPLC/UHPLC GL SCIENCES HPLC COLUMN CATALOG Please contact your sales representative for a copy of the new GL Sciences catalogue. E-56 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE