HPLC Catalogue

HPLC/UHPLC Columns ACE® Analytical HPLC Column Ordering Information - (ACE & ACE Excel C18) Dimensions ACE® & ACE® Excel™ 100Å C18 Analytical HPLC Columns HPLC/UHPLC Columns ID x Length (mm) 5 µm 3 µm 2 µm 1.7 µm 4.6 x 250 ACE-121-2546 -- - 4.6 x 150 ACE-121-1546 - 4.6 x 100 ACE-121-1046 ACE-111-1546 EXL-101-1546U - 4.6 x 75 ACE-121-7546 - 4.6 x 50 ACE-121-0546 ACE-111-1046 EXL-101-1046U - 4.6 x 30 ACE-121-0346 - 4.6 x 20 ACE-121-0246 ACE-111-7546 EXL-101-7546U - 3.0 x 250 ACE-121-2503 - 3.0 x 150 ACE-121-1503 ACE-111-0546 EXL-101-0546U - 3.0 x 100 ACE-121-1003 EXL-171-1003U 3.0 x 75 ACE-121-7503 ACE-111-0346 EXL-101-0346U EXL-171-7503U 3.0 x 50 ACE-121-0503 EXL-171-0503U 3.0 x 30 ACE-121-0303 ACE-111-0246 EXL-101-0246U EXL-171-0303U 3.0 x 20 ACE-121-0203 EXL-171-0203U 2.1 x 250 ACE-121-2502 -- - 2.1 x 150 ACE-121-1502 - 2.1 x 100 ACE-121-1002 ACE-111-1503 EXL-101-1503U EXL-171-1002U 2.1 x 75 ACE-121-7502 EXL-171-7502U 2.1 x 50 ACE-121-0502 ACE-111-1003 EXL-101-1003U EXL-171-0502U 2.1 x 30 ACE-121-0302 EXL-171-0302U 2.1 x 20 ACE-121-0202 ACE-111-7503 EXL-101-7503U EXL-171-0202U 1.0 x 250 ACE-121-2501 - 1.0 x 150 ACE-121-1501 ACE-111-0503 EXL-101-0503U - 1.0 x 100 ACE-121-1001 - 1.0 x 75 ACE-121-7501 ACE-111-0303 EXL-101-0303U - 1.0 x 50 ACE-121-0501 - ACE-111-0203 EXL-101-0203U -- ACE-111-1502 EXL-101-1502U ACE-111-1002 EXL-101-1002U ACE-111-7502 EXL-101-7502U ACE-111-0502 EXL-101-0502U ACE-111-0302 EXL-101-0302U ACE-111-0202 EXL-101-0202U -- ACE-111-1501 - ACE-111-1001 - ACE-111-7501 - ACE-111-0501 - Semi-Prep and 10 µm also available, please visit lifescience.ca ACE® Analytical HPLC Guard Cartridge Ordering Information Dimensions ACE® 100Å C18 ID x Length (mm) 5 µm 3 µm Cartridges for 10 x 2.1mm ID Columns (pkg of 5) ACE-121-0102GD ACE-111-0102GD Cartridges for 10 x 3.0 - 4.6mm ID Columns (pkg of 5) ACE-121-0103GD ACE-111-0103GD Integral guard cartridge holder for 2.1mm ID Columns ACE-H0004 Integral guard cartridge holder for 3.0 - 4.6mm ID Columns ACE-H0005 Stand-alone universal guard cartridge holder. Requires Column coupler item # ACE-C0001 ACE-H0001 Other dimensions are available. Please enquire for details. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN A-9 LIFE SCIENCE

Colonnes HPLC/UHPLC ACE® & ACE® Excel™ C18-AR ACE UHPLC and HPLC Columns Colonnes HPLC/UHPLC Physical Properties Leveraging ACE Excel C18-AR Selectivity Silica : Ultra-Inert Base Deactivated Conditions: Analytes: Particle SizAe nalge: s1i.c7 Sµmep, a2rµamti,o3nµm, 5 µm Part Number: EXL-109-0502U 1. Pyridoxine Surface Area : 300 m²/g 1A. An4pa-pAlylcicteaetMTFGBtaeslirmo=oomawnbdi2dpii0#olReeeApnarmaPhttNetM:ehu2:2nar400osKe9.elH:3t0:o412AP07mO=0°4lC%/2, mp0BHimnin2M.57K/mMHi2nePuOOtHe4,s(p5H0:25.07va/nvd) 2. p-Aminobenzoic acid PBoornedSeidzeP hCaosen diti::o1On0cs0taÅdecyl with phenyl groups 2. 4-AminDoebteenctziooinc:aUciVd, 205 nm 3. Pantothenic acid CEnardb-CoanpLpoianPDCdgaoimr ltueNmnusnmi:o::bn1Yese:5r:s.5A1A%5CC0EE-x3114C9.61-18m5-4Am6R 3. 4-HydrCooxlyubmennzDoiicmaecnidsions: 50 x 2.1mm 4. Folic acid USP Code Mobile Ph: aLs1e: A: 0.1% formic acid in H2O 5. D-Biotin pH Range : 2.0-8B.:00.1% formic acid in MeCN 4. Caffeine 6. Cyanocobalamin Gradient: Time (mins) %B 5. 2-Acetamidophenol 7. Riboflavin 6. 3-Hydroxybenzoic acid 1 05 7. Salicylamide 4 6 9 35 8. Acetanilide 3 2 ACE Excel 2 C18-AR 5 Pmax = 209 bar Mechanisms of Separa14tion Stre3n5gth of Interact9i.onPhenol π-π interaFTceltomiwopneRsraatteu:re: 1 mL/min 10. Acetylsalicylic acid 40 °C Strong 11. Benzoic acid For additional7 12. Sorbic acid Dipole-dipDoelteecitniotne:ractiUoVn, s240 nmModerate 13. Salicylic acid 0 column2 3 dim4 ensio5ns 111 14. Phenacetin Minutes Hydrophobic binding 15. Salicylaldehyde 1 6 interactions Strong 1144 Please enquire 113 email: [email protected] Shape selectivity Modera88te 77 11 33 1100 1122 Recommended Applications 66 • Analytes differing in hydrophobicity, homologous Target Analytes • Analytes with π bonding55 compounds differing by –CH2 • Stereoisomers • Awnitahldytreaswwinigthgeroleucptsro, snu4d4cehloascahliazlaotgioennsa,nnditerolegcrtorounp-s, 1155 • Steroids ketones, este22rs, and acids 99 • Analytes with different dipole moments • Taxanes • Analytes differing in hydrophobicity 12 min • Substituted aromatics • Polar, m4 odera5 tely p6 olar, a7 nd no8T nim-ep-oMl9ian urteasna10ly tes 11 • Highly aqueous conditions • Uncharged acids and bases • Particularly recommended for applications where a typical C18 does not provide an adequate separation Analgesics / Cough & Cold Medicine Ingredients Analgesics / Cough & Cold Medicine Ingredients Application #AN1930 Conditions Analytes mAU 11 PCADCMBGaoo::irmoar0nl0utbd..de1m1Niiilne%%teunisnomiP:tffono:ohAAnbsrraCCs:emms:EEreT0is1iMCDPGc33c::i5amo iraAAaoCCm0ralcctCCebu11dxiieEEdN88imdi(lne4--me-ui11iAsnn.nn6mP11ii:RtonM:hH19msbn--a2)ees11Oms:rC55es44N::%566AA1AAA90TBB5iCCCC::m000EEEE..e--x11331111%%(4mCC19.6-11-1ffi188oon5m5-rrs44Ammm)66Riicc HM2eOCN869141A12537...1..0....nA3CP2S44P..aaa----ABhacl32569711841HHAAyl.ref.e1e0s.......itfacmc.yyptnne.eceayddiioz2P4PCA3ASB4sierlnntrrno:i----ialhaacesatcooAHAHneioiarllepefmncixxiafcamcyybmdtinezyycreaddcyaioeeioibbdienintolncrrninidlaooeeoctcieilodalzmxxnnipbaadomyyzzhccieeibbdoocoieinddeeoiilnzaccnnpooczzaahiiloocdcceiiiinccaddocaaildcciidd 22 77 88 1111 9 3514 1133 1144 11,,22 33 acid in 55 66 1100 1122 1155 acid in 99 %B 44 5 ACE C18-AR 35 33,,44 35 55 66 1133,,1144 1111,,1122 14 3515 5 12. S1o2r.bSicorabcicidacid 1F5low Rate: 51 mL/min 13. S1a3l.icSyalilcicyalcicidacid Flow Rate: 1 mTeLm/mpeinrature: 40 °C 14. P1h4e. nPahceentaicnetin 88 Temperature:D4e0te°cCtion: UV, 240 nm 15. S1a5l.icSyalalicldyelahldyedheyde 77 Detection: UV, 240 nm 1100 99 1155 ACE C18 4 5 6 7 8 9 10 11 12 min Time - Minutes A-10 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns ACE® Analytical HPLC Column Ordering Information - (ACE & ACE Excel C18-AR) Dimensions ACE® & ACE® Excel™ 100Å C18-AR Analytical HPLC Columns* HPLC/UHPLC Columns ID x Length (mm) 5 µm 3 µm 2 µm 1.7 µm 4.6 x 250 ACE-129-2546 ACE-119-2546 - - 4.6 x 150 ACE-129-1546 - 4.6 x 100 ACE-129-1046 ACE-119-1546 EXL-109-1546U - 4.6 x 75 ACE-129-7546 - 4.6 x 50 ACE-129-0546 ACE-119-1046 EXL-109-1046U - 4.6 x 30 ACE-129-0346 - 4.6 x 20 ACE-129-0246 ACE-119-7546 EXL-109-7546U - 3.0 x 250 ACE-129-2503 - 3.0 x 150 ACE-129-1503 ACE-119-0546 EXL-109-0546U - 3.0 x 100 ACE-129-1003 EXL-179-1003U 3.0 x 75 ACE-129-7503 ACE-119-0346 EXL-109-0346U EXL-179-7503U 3.0 x 50 ACE-129-0503 EXL-179-0503U 3.0 x 30 ACE-129-0303 ACE-119-0246 EXL-109-0246U EXL-179-0303U 3.0 x 20 ACE-129-0203 EXL-179-0203U 2.1 x 250 ACE-129-2502 ACE-119-2503 - - 2.1 x 150 ACE-129-1502 - 2.1 x 100 ACE-129-1002 ACE-119-1503 EXL-109-1503U EXL-179-1002U 2.1 x 75 ACE-129-7502 EXL-179-7502U 2.1 x 50 ACE-129-0502 ACE-119-1003 EXL-109-1003U EXL-179-0502U 2.1 x 30 ACE-129-0302 EXL-179-0302U 2.1 x 20 ACE-129-0202 ACE-119-7503 EXL-109-7503U EXL-179-0202U 1.0 x 250 ACE-129-2501 - 1.0 x 150 ACE-129-1501 ACE-119-0503 EXL-109-0503U - 1.0 x 100 ACE-129-1001 - 1.0 x 75 ACE-129-7501 ACE-119-0303 EXL-109-0303U - 1.0 x 50 ACE-129-0501 - ACE-119-0203 EXL-109-0203U ACE-119-2502 - ACE-119-1502 EXL-109-1502U ACE-119-1002 EXL-109-1002U ACE-119-7502 EXL-109-7502U ACE-119-0502 EXL-109-0502U ACE-119-0302 EXL-109-0302U ACE-119-0202 EXL-109-0202U ACE-119-2501 - ACE-119-1501 - ACE-119-1001 - ACE-119-7501 - ACE-119-0501 - Semi-Prep and 10 µm also available, please visit lifescience.ca ACE® Analytical HPLC Guard Cartridge Ordering Information Dimensions ACE® 100Å C18-AR ID x Length (mm) 5 µm 3 µm Cartridges for 10 x 2.1mm ID Columns (pkg of 5) ACE-129-0102GD ACE-119-0102GD Cartridges for 10 x 3.0 - 4.6mm ID Columns (pkg of 5) ACE-129-0103GD ACE-119-0103GD Integral guard cartridge holder for 2.1mm ID Columns ACE-H0004 Integral guard cartridge holder for 3.0 - 4.6mm ID Columns ACE-H0005 Stand-alone universal guard cartridge holder. Requires Column coupler item # ACE-C0001 ACE-H0001 Other dimensions are available. Please enquire for details. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN A-11 LIFE SCIENCE

Colonnes HPLC/UHPLC ACE UHPLC and HPLC Columns Catecholamines and their Metabolites in Urine by LC-MS/MS ACE® & ACE® Excel™ C18-PFP Conditions Analytes Colonnes HPLC/UHPLC Column: ACE UltraCore 2.5 1. Norepinephrine Physical Properties LeveragDinimgetnhseionusn: iqS1u0ue0peAxrPC2h.E1enmEyxmlHceexlyCl 18-PFP selectivity2. (m/z 170 107) Epinephrine Silica : Ultra-Inert Base Deactivated Particle Size : 1.7 µm, 2 µm, 3 µm, 5 µm CCoonludmitnio:nAPMsCa:oErtbENilxecuemPlh2baeCsre1::8-CAPO:F2PRmE-M25Bam-10m0o2nUium formA1.na1tae,l2y+,t3e0-sT.:0ri5m%ethox3y. bNeonrmz(meen/ztea1n8e4phri1n6e6) Surface Area : 300 m²/g Dimensions: 50 x 2.1mm formic acid in H2O 2. 1,2-Dimethoxybenzen(me/z 184 166) Part Number: EXL-1010-0B5:022UmM ammonium form3.a1te,2+,40-T.0ri5m%ethox4y. bDeonpzaemniene Pore Size : 100 Å Mobile Phase: MeOH/H2Ofor(m50ic:5a0civd/ivn) MeOH 4. Methoxybenzene (m/z 154 91) Bonded Phase : O ctadecyl with pentaflurophenyl groups Flow RateG: r0a.2d1iemntl:/min Time (mins) %B 5. 1,3-Dimethoxyb5e. nMzeetnaenephrine Temperature: 40 °C 0.00 0 6. 1,3,5-Trimethoxybenz(men/ze198 180) End-Capping : Yes 1.00 70 7. Toluene 6. 3-Methoxytyramine Carbon Load : 14.3% USP Code : L1 1.10 70 (m/z 181 91) pH Range : 2.0-8.0 1.11 0 ACE Excel 2FlCo1w8-PRFaPte(f:ully 4.50 0 37.5e5 porous ultIrnajiencetritosnili:ca) 35.0e5 0.3 mL/min 32.5e5 Temperature: 30.0e5 Detection: 10 µL 6 27.5e5 Sample: 310 °C 7 25.0e5 Shima3dzu LCMS-8040 22.5e5 20.0e5 Mechanisms of Separation Strength of Interaction ESI in positive ion mode π-π interactions Strong Dipole-dipole interactions Strong Standard 100 ng/mL in urine 5 Hydrophobic binding interactions Strong (aft2er SPE purification4) Shape selectivity Strong 17.5e5 15.0e5 12.5e5 0 1 2 3 4 5 6 710.0e5 Minutes 7.5e5 5.0e5 2.5e5 Target Analytes Recommended Applications 0.0 • Analytes with π bonding • Analytes with electron donating groups, such as • RepSrotedurceeodiswoitmh peerrsmission of Shimadzu, France phenols, aromatic ethers and amines • Steroids • Analytes with proton donor groups • CaTtaexcahnoeslamines and Metanephrines Separation (Gradient) • Analytes with different dipole moments • Analytes differing in hydrophobicity • Substituted aromatics • Polar, moderately polar, and non-polar analytes • Uncharged acids and bases • CoAnndaitlyiotenssdiffering in hydrophobicity, homAonlaolgyotues Colcuommn:poundAsCEdi5ffCe1r8in-PgFPby –CH2 1. Norepinephrine • PDaimrHteNingushmiolybnesa:r:quA1e5Co0Eu-x1s241.m60-mo15mb4i6le phase conditions32.. Epinephrine Normetanephrine • MoPbialertPichuaslea:rlAy:r2e0cmoMmmamemnodneiudmffoorrmaapteplicatio4n.sDwophamerinee a typical C18 does npHot3.p0rionvHi2dOe an adequate separ5a.tMioentanephrine No B: 20 mM ammonium formate Nor Catecholamines and Metanephrines Separation (Gradient) Gradient: pH 3.0 in MeOH/H2O (90:10 v/v) Time (mins) %B 00 5 40 44 Conditions: Analytes: 6 40 33 Column: ACE 5 C18-PFP 1. Norepinephrine 70 2. Epinephrine Dimensions: 150 x 4.6 mm 3. Normetanephrine 17 0 5 4. Dopamine 1 mL/min Part Number: ACE-1210-1546 5. Metanephrine Flow Rate: Mobile Phase:  Injection: 5 µL A: 20 mM ammonium formate Temperature: 25 °C pH 3.0 in H2O Detection: UV, 260 nm B: 20 mM ammonium formate 22 pH 3.0 in MeOH/H2O (90:10 v/v) Gradient: Time (mins) %B 11 00 5 40 6 40 M 70 17 0 Flow Rate: 1 mL/min Injection: 5 μL Temperature: 25 °C Detection: UV, 260 nm 1 2 3 4 5 6 min Time - Minutes A-12 CANADIAN 1 888-2265-42775 w:w: [email protected]|.c+a44::(0)w1w22w4.l7if0e4sc5ie5n4ce|[email protected] LIFE SCIENCE

HPLC/UHPLC Columns ACE® Analytical HPLC Column Ordering Information - (ACE® & ACE® Excel™ C18-PFP) Dimensions ACE® & ACE® Excel™ 100Å C18-PFP Analytical HPLC Columns HPLC/UHPLC Columns ID x Length (mm) 5 µm 3 µm 2 µm 1.7 µm 4.6 x 250 ACE-1210-2546 -- - 4.6 x 150 ACE-1210-1546 - 4.6 x 100 ACE-1210-1046 ACE-1110-1546 EXL-1010-1546U - 4.6 x 75 ACE-1210-7546 - 4.6 x 50 ACE-1210-0546 ACE-1110-1046 EXL-1010-1046U - 4.6 x 30 ACE-1210-0346 - 4.6 x 20 ACE-1210-0246 ACE-1110-7546 EXL-1010-7546U - 3.0 x 250 ACE-1210-2503 - 3.0 x 150 ACE-1210-1503 ACE-1110-0546 EXL-1010-0546U - 3.0 x 100 ACE-1210-1003 EXL-1710-1003U 3.0 x 75 ACE-1210-7503 ACE-1110-0346 EXL-1010-0346U EXL-1710-7503U 3.0 x 50 ACE-1210-0503 EXL-1710-0503U 3.0 x 30 ACE-1210-0303 ACE-1110-0246 EXL-1010-0246U EXL-1710-0303U 3.0 x 20 ACE-1210-0203 EXL-1710-0203U 2.1 x 250 ACE-1210-2502 -- - 2.1 x 150 ACE-1210-1502 - 2.1 x 100 ACE-1210-1002 ACE-1110-1503 EXL-1010-1503U EXL-1710-1002U 2.1 x 75 ACE-1210-7502 EXL-1710-7502U 2.1 x 50 ACE-1210-0502 ACE-1110-1003 EXL-1010-1003U EXL-1710-0502U 2.1 x 30 ACE-1210-0302 EXL-1710-0302U 2.1 x 20 ACE-1210-0202 ACE-1110-7503 EXL-1010-7503U EXL-1710-0202U 1.0 x 250 ACE-1210-2501 - 1.0 x 150 ACE-1210-1501 ACE-1110-0503 EXL-1010-0503U - 1.0 x 100 ACE-1210-1001 - 1.0 x 75 ACE-1210-7501 ACE-1110-0303 EXL-1010-0303U - 1.0 x 50 ACE-1210-0501 - ACE-1110-0203 EXL-1010-0203U -- ACE-1110-1502 EXL-1010-1502U ACE-1110-1002 EXL-1010-1002U ACE-1110-7502 EXL-1010-7502U ACE-1110-0502 EXL-1010-0502U ACE-1110-0302 EXL-1010-0302U ACE-1110-0202 EXL-1010-0202U -- ACE-1110-1501 - ACE-1110-1001 - ACE-1110-7501 - ACE-1110-0501 - Semi-Prep and 10 µm also available, please visit lifescience.ca ACE® Analytical HPLC Guard Cartridge Ordering Information Dimensions ACE® 100Å C18-PFP ID x Length (mm) 5 µm 3 µm Cartridges for 10 x 2.1mm ID Columns (pkg of 5) ACE-1210-0102GD ACE-1110-0102GD Cartridges for 10 x 3.0 - 4.6mm ID Columns (pkg of 5) ACE-1210-0103GD ACE-1110-0103GD Integral guard cartridge holder for 2.1mm ID Columns ACE-H0004 Integral guard cartridge holder for 3.0 - 4.6mm ID Columns ACE-H0005 Stand-alone universal guard cartridge holder. Requires Column coupler item # ACE-C0001 ACE-H0001 Other dimensions are available. Please enquire for details. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN A-13 LIFE SCIENCE

Reproduced with permission of Nufarm UK Ltd Phenolic Compounds in Ground Water & Landfill Leachates ColonnePsheHAnCoPlEiLcCUAc/HiUdPsLHCPaLnCd HPLC Columns Application #AN3070 Application #AN3030 Conditions Analytes Conditions Analytes ACE® ECxolcuemOnl™:rgaCn1iAc8CAE-Ac5iCdm1s8ide 1. Caffeic acid Column: ACE Excel 3 C18-Amide 1. Pyrocatechol Dimensions: 150 x 4.6 mm 2. p-CouAmpaprilcicaactiiodn #AN2780 Dimensions: 150 x 4.6 mm 2. Resorcinol Colonnes HPLC/UHPLC Physical PPMraoortbpNileeuCrmPtohibneaesdsre:i:tioAMCneEsC-N12/01-.115%46formic acid in 3. Ferulic acid PMPhaoretbnNileoulmPihcbaeCsreo::mE0.pX1Lo%-u11fno1r2dm-1si5ci4na6cUGidrvo/vuinnHd2OWate34..r &Pmh-LeCnareonsl dolfill Leachates Response - MilliVolts Analytes PSSPBuiaoolirrrnctfeadiac Sceleeidz eASP DTIFirnhzeleeojemaetaw ecsp cteeRito CMDPFiraoanlaoitoonmtr:elwutbu::e:::::rNimlenRe1U1O3u:nsamP0.0i:ltco7the00HUA11tbnr:aaµesam2VµmsÅmO:dr,-emL:bL2I:e²(in/5e/2,mcA25A1H4eng02y05CC2ti:mrnnO80lEEmtµm0L/w-xM51BM/mvm24iCe/a1t.viK6,On1-hs)2H83eHm52a4Pmµ(Dm67Om0e4:i3adp,0Hce5tv5i/gµvv.7r)amointuepd51234s..... CDPCMDTIFnaooeilemoojrlnmeuttbwdeecmNipicltnteeRiutnisooiramiP:oanonthAnt:nbesuaC:s::ers:Eree1::E:E5x0X0c.13M1UL1ex00V%lm-e,41µ3°CL2.C1fL6CNo/71m41rm2(m86in-nm-15imAc5:3m4a5c6idivUde/vv)/v in H2O 638A57412........n9615171183mPoRP32.1a023.....,,,hye-l...445-yCrseC2233223o1---toronDDD-e-r,,-,,,,ecNN4C44543reosiiisacmmm-,,,--saa:rolDD656Diteopnpel---eeeiisiTTTlhhommmctttorrrtthlhhhiiihhlmmmeeeoyyyootttlllleeehhhllpppyyyttthhhhhhlllpppeeeyyyhhhlllnnnpppeeeooohhhnnnllleeeooonnnlll ooolll L -Ascorbic acid MeCN (65:35 v/v) Maleic acid Acetylsalicylic acid Flow Rate: 1 mL/min Benzoic acid Injection: 10 μL Salicylic acid Temperature: 30 °C End-Capping Temp:eYreatsure: 22 °C Carbon Load Dete:ct1io6n.:4% UV, 220 nm USP Code : L1 & L60 Detection: UV, 274 nm 9. 1-Naphthol pH Range : 2.0-8.0 For further applications10. 3,4,5-Trimethylphenol 1 11. 2,3,6-Trimethylphenol Mechanisms of SLe-pACsaa2cfrfoearibctiicao3canicdid p-CMouamleaicriaccaidcid FAecreutlyiclsaacliicdylic acid mmAAUU 11 visit: www.ace-h1123p.. 22lc,-4N.,a6cp-oThrtimhmoelthylphenol St1rength of Interaction 22 or 14 π-π interactions Strong 12 email: [email protected] Dipole-dipole interBaecntziooicnascid StrongSalicylic acid22 3 4 10 Hydrophobic binding Strong interactions 3 2 8 6 44 5 1100 4 99 11111122 Shape selectivity Strong1 35 2 66 77 88 133 0 Target Analytes Recommended Applications -2 • Organic acids •T ime -SMminautlelswater soluble 5 10 15 20 25 min Time - Minutes • Phenolic compounds analytes Reproduced with permission of Environmental Scientific Group Ltd, UK • Stero1i3d6s 0.0ww0w.5 .ac1e.0-hp1.l5c.co2.m0 |aH2T.n5+i-mbd4eo4-p3nM.o(0di0nlua)dtre1o3sc.25no2om4r4sp7.,0o0au4cni45d.d55ss,4 5|.0 [email protected] • • bases Vitamin D2/D3 Organic Acids – Fast Saenpdaprhaetinoonlic compounds • Small peptides Application #AN2200 Conditions Analytes Column: ACE Excel 3 C18-Amide 1. Sinapic acid ACE Excel 1.7 C18-Amide 2. Ferulic acid Organic Acids PD–aimrFtaeNnsutsmioSbneesp:r:ar22a55t00ioxxn22..11 mm, 50 x 3.0 mm 3. 3,4-Dimethoxycinnamic acid mmAAUU 11 22 33 ACE 3 Excel C18-Amide mm (EXL -1112-2502U), 4. Cinnamic acid 220000 44 50 x 3 mm (EXL -1712-0503U) 5. 4-Methoxycinnamic acid 55 Conditions: Mobile Phase: 20 mM H3PO4 in Analytes: 117755 2200 25 3300 3355 4400 min Column: ACE Excel 3 C18-Amide MeOH/H2O (40:60 v/v) 1. Sinapic acid ACE ExceFl l1o.w7 CR1a8te-A: mide0.21 mL/min (250 x 2.1 mm)2. Ferulic acid 115500 Dimensions: 250 x 2.1 mm, 50 x 30..08mmmL/min (50 x 3.0 mm) 3. 3,4-Dimethoxycinnamic acid 112255 Part Numbers: 2 50Inxje2c.t1iomn:m (EXL5-µ1L11(225-025x022.U1 )m, m) 4. Cinnamic acid 110000 50 x 3 mm (EXL -1721µ2L-0(5500x3U3.)0 mm) 5. 4-Methoxycinnamic acid 7755 Mobile Phase: 20 TmeMmpHe3raPtOu4rei:n M20eO°CH/H2O (40:60 v/v) Flow Rate: 0 .21 mDL/emteicnti(o2n5:0 x 2.1UVm, 2m1)0 nm 5500 0.8 mL/min (50 x 3.0 mm) 2255 ACE 3 C18-Amide Injection: 5 μL (250 x 2.1 mm) Sinapic acid 00 2 μL (50 x 3.0 mm) Temperature: 20 °C I 0 55 1100 1155 Detection: UV, 210 nm mmAAUU 44 ACE Excel 1.7 C18-Amide 117755 ACE Excel 1.7 C18-Amide 1100 1155 2200 2255 330 3355 4400 min Ferulic acid 3,4-Dimethoxycinnamic 115500 acid Time - Minutes 112255 22 33 110000 11 7755 55 5500 2255 Cinnamic acid 4-Methoxycinnamic 00 55 acid I 0 A-14 112CANwwAwD.aIAceN-hplc.com | +44 (0) 1224 704 5158488| -i2n2fo6@-27a7ce5-hp::[email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns ACE® Analytical HPLC Column Ordering Information - (ACE® Excel™ C18-Amide) Dimensions ACE® Excel™ 100Å C18-Amide Analytical HPLC Columns HPLC/UHPLC Columns ID x Length (mm) 5 µm 3 µm 2 µm 1.7 µm 4.6 x 250 EXL-1212-2546U -- - 4.6 x 150 EXL-1212-1546U - 4.6 x 100 EXL-1212-1046U EXL-1112-1546U EXL-1012-1546U - 4.6 x 75 EXL-1212-7546U - 4.6 x 50 EXL-1212-0546U EXL-1112-1046U EXL-1012-1046U - 4.6 x 30 EXL-1212-0346U - 4.6 x 20 EXL-1212-0246U EXL-1112-7546U EXL-1012-7546U - 3.0 x 250 EXL-1212-2503U - 3.0 x 150 EXL-1212-1503U EXL-1112-0546U EXL-1012-0546U - 3.0 x 100 EXL-1212-1003U EXL-1712-1003U 3.0 x 75 EXL-1212-7503U EXL-1112-0346U EXL-1012-0346U EXL-1712-7503U 3.0 x 50 EXL-1212-0503U EXL-1712-0503U 3.0 x 30 EXL-1212-0303U EXL-1112-0246U EXL-1012-0246U EXL-1712-0303U 3.0 x 20 EXL-1212-0203U EXL-1712-0203U 2.1 x 250 EXL-1212-2502U -- - 2.1 x 150 EXL-1212-1502U - 2.1 x 100 EXL-1212-1002U EXL-1112-1503U EXL-1012-1503U EXL-1712-1002U 2.1 x 75 EXL-1212-7502U EXL-1712-7502U 2.1 x 50 EXL-1212-0502U EXL-1112-1003U EXL-1012-1003U EXL-1712-0502U 2.1 x 30 EXL-1212-0302U EXL-1712-0302U 2.1 x 20 EXL-1212-0202U EXL-1112-7503U EXL-1012-7503U EXL-1712-0202U 1.0 x 250 ACE-1212-2501 - 1.0 x 150 ACE-1212-1501 EXL-1112-0503U EXL-1012-0503U - 1.0 x 100 ACE-1212-1001 - 1.0 x 75 ACE-1212-7501 EXL-1112-0303U EXL-1012-0303U - 1.0 x 50 ACE-1212-0501 - EXL-1112-0203U EXL-1012-0203U -- EXL-1112-1502U EXL-1012-1502U EXL-1112-1002U EXL-1012-1002U EXL-1112-7502U EXL-1012-7502U EXL-1112-0502U EXL-1012-0502U EXL-1112-0302U EXL-1012-0302U EXL-1112-0202U EXL-1012-0202U ACE-1112-2501 ACE-1012-2501 ACE-1112-1501 ACE-1012-1501 ACE-1112-1001 ACE-1012-1001 ACE-1112-7501 ACE-1012-7501 ACE-1112-0501 ACE-1012-0501 Semi-Prep and 10 µm also available, please visit lifescience.ca ACE® Analytical HPLC Guard Cartridge Ordering Information Dimensions ACE® 100Å C18-Amide ID x Length (mm) 5 µm 3 µm Cartridges for 10 x 2.1mm ID Columns (pkg of 5) ACE-1212-0102GD ACE-1112-0102GD Cartridges for 10 x 3.0 - 4.6mm ID Columns (pkg of 5) ACE-1212-0103GD ACE-1112-0103GD Integral guard cartridge holder for 2.1mm ID Columns ACE-H0004 Integral guard cartridge holder for 3.0 - 4.6mm ID Columns ACE-H0005 Stand-alone universal guard cartridge holder. Requires Column coupler item # ACE-C0001 ACE-H0001 Other dimensions are available. Please enquire for details. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN A-15 LIFE SCIENCE

Conditions Analytes Colonnes HPLC/UHPLC Column: ACE 5 SuperC18 1. Omeprazole Dimensions: 150 x 4.6 mm 2. Lansoprazole Part Number: EXL -1211-1546U Mobile Phase: A: 0.1% ammonia in H2O B: 0.1% ammonia in MeCN/H2O (90:10 v/v) ACE® Excel™ SuperC18 Anti-UlcGerardDiernut:gs in B0Tiamseic(mMinosb) ile1%0PBhase Conditions Colonnes HPLC/UHPLC 5 90 Physical Properties ConditionFsl:ow Rate: 1 mL/min Analytes: Column: AInCjEecEtxiocenl: 5 Supe5rCµ1L8 1. Omeprazole Silica : Ultra-Inert Base Deactivated DimensioTnesm: 1p5e0raxt4u.r6e:mm30 °C 2. Lansoprazole Particle Size : 1.7 µm, 2 µm, 3 µm, 5 µm Part NumDbeert:ecEtXiLo-n1:211-1U5V4,62U80 nm Mobile Phase: 2 Surface Area : 400 m²/g A: 0.1% ammonia in MH2eOCN/H2O 1 Pore Size : 90 Å B: 0.1% ammonia in Bonded Phase : Octadecyl encapsulated (90:10 v/v) Gradient: Time (mins) %B End-Capping : Yes 0 10 Carbon Load : 14.8% 5 Omepra9z0ole USP Code : L1 Flow Rate: 1 mL/min Injection: 5 μL pH Range : 1.5-11.5 Temperature: 30 °C Detection: UV, 280 nm Mechanisms of Separation Strength of Interaction Lansoprazole Hydrophobic binding Strong interactions Weak 12345 Shape selectivity Time - Minutes www.ace-hplc.com Target Analytes Recommended Applications • Analytes differing in hydrophobicity • Analytes differing in hydrophobicity • Polar, moderately polar, and non-polar analytes • Homologous compounds differing by –CH2 • Uncharged acids and bases • Ionized acids or bases using ion-pairing Encapsulated Bonding Technology (EBT™) for Improved Chromatography and Stability Traditional C18 Bonding ACE SuperC18 with Encapsulated Bonding Technology (EBT™) C18 Endcapping group C18 Unbonded silanol group A-16 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE





























































HPLC/UHPLC Columns Diazepam Metabolites ACME C18 Flow rate: 0.3 mL/min Sample: 4 HPLC/UHPLC Columns 1.9 µm, 100 x 2.1 mm Temperature: 25 °C 1. Oxazepam Isocratic Mobile Phase: Detection: UV@254 nm 2. Nordiazepam A: 0.1% formic acid in water Injection Vol.: 1 µL 3. Temazepam B: 0.1% formic acid in acetonitrile 4. Diazepam A:B, (60:40, v/v) 2 3 1. Oxazepam 2. Nordiazepam 1 IMPURITY 3. Temazepam 4. Diazepam 1.02 02.0 3.04 04.0 5.06 06.0 7.0 Time (min) Paracetamol / Aspirin Mix 1. Acetaminophen 2. Caffeine 3. Salicylamide ACME C18 4. Acetylsalicylic Acid 5. Benzoic Acid 6. Salicylic Acid 1.9 µm, 50 x 2.1 mm 12 4 5 Isocratic Mobile Phase: A: 0.1% formic acid in water 6 B: Methanol A:B, (65:35, v/v) Flow rate: 0.25 mL/min Temperature: 22 °C Detection: UV@240 nm Injection Vol.: 2 µL Sample: 1. Acetaminophen (Paracetamol, Tylenol) 2. Caffeine (1,3,7-Trimethylxanthine) 3. Salicylamide (o-Hydroxybenzamide) 4. Acetylsalicylic Acid (ASA, Aspirin) 5. Benzoic Acid 6. Salicylic Acid 3 1.02 02 .0 3.04 04 .0 5.06 06 .0 Time (min) B-7 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN LIFE SCIENCE

Colonnes HPLC/UHPLC Colonnes HPLC/UHPLC ACME Cannabis Physical Properties: SBeopnadreadtioPnhaMseo:d-eC:18RHe3v7e–rXsed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 450 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 100 Å Carbon Load (%): 22 Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: ≤ 98% USP Listing: L1 ACME Cannabis is a C18 bonded phase (L1) with optimized pore structure and phase loading for the separation of cannabinoids and cannabinoid-like compounds. It can also be used in applications where more retention is required when compared to the ACME C18. Dimensions 1.9 μm ACME Cannabis - 100 Å 5 μm Length (mm) x ID ACMCB-1.9-03021 3 μm ACMCB-5-03021 2.1 x 30 ACMCB-1.9-03046 ACMCB-5-03046 4.6 x 30 ACMCB-1.9-05021 ACMCB-3-03021 ACMCB-5-05021 2.1 x 50 ACMCB-1.9-05030 ACMCB-3-03046 ACMCB-5-05030 3.0 x 50 ACMCB-1.9-05046 ACMCB-3-05021 ACMCB-5-05046 4.6 x 50 ACMCB-1.9-10021 ACMCB-3-05030 ACMCB-5-10021 2.1 x 100 ACMCB-1.9-10030 ACMCB-3-05046 ACMCB-5-10030 3.0 x 100 ACMCB-1.9-10046 ACMCB-3-10021 ACMCB-5-10046 4.6 x 100 ACMCB-1.9-15021 ACMCB-3-10030 ACMCB-5-15021 2.1 x 150 ACMCB-3-10046 ACMCB-5-15030 3.0 x 150 - ACMCB-3-15021 ACMCB-5-15046 4.6 x 150 - ACMCB-3-15030 ACMCB-5-25046 4.6 x 250 - ACMCB-3-15046 - ACME Cannabis - 100 Å Guard Columns Dimensions 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder ID x Length (mm) 2.1 x 5 ACMGC19UC521CB ACMGC3LC521CB ACMGC5LC521CB 3.0/4.6 x 5 ACMGC5LC546CB 2.1 x 10 ACMGC19UC546CB ACMGC3LC546CB ACMGC5LC1021CB 3.0/4.6 x 10 ACMGC5LC1046CB ACMGC19UC1021CB ACMGC3LC1021CB ACMGC19UC1046CB ACMGC3LC1046CB Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-8 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Cannabinoids ACME Cannabis Sample: 6. Cannabidiol (CBD) HPLC/UHPLC Columns 3 µm, 150 x 4.6 mm 1. Cannabidivarinic Acid (CBDVA) 7. Tetrahydrocannabivarin (THCV) A: 0.1% Formic Acid in Water, (v/v) 2. Cannabidivarin (CBDV) 8. Cannabinol (CBN) B: 0.095% Formic Acid in Acetonitrile, (v/v) 3. Cannabidiolic Acid (CBDA) 9. Δ9-tetrahydrocannabinol (THC Δ9) Gradient: 4. Cannabigerolic Acid (CBGA) 10. Δ8-tetrahydrocannabinol (THC Δ8) 5. Cannabigerol (CBG) 11. Cannabichromene (CBC) Profile Time AB 4. CBGA (min) 0 30 70 10 10 90 15 10 90 Flow rate: 1.5 mL/min 5. CBG 6. CBD Temperature: 25 °C Detection: UV@225 nm Injection Vol.: 2 μL 7. THCV 8. CBN 9. THC Δ9 1. CBDVA 8 2. CBDV 3. CBDA 10. THC Δ8 11. CBC 11 2 3 9 1 56 10 4 7 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-9 LIFE SCIENCE

Colonnes HPLC/UHPLC Colonnes HPLC/UHPLC ACME F5/C18 Physical Properties • F5/C18 can operate in 100% aqueous conditions and shows SBeopnadreadtioPnhaMseo:d-eC:18RHe3v7e–rXs-ePdF-PPhase improved retention of polar compounds Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm • Ultra-high purity (99.999%) extra-treated porous spherical Surface Area: 260 m2/g silica, for excellent peak shape and reproducibility Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 120 Å • Ultra-low bleed phase is suitable for use with UV and MS Carbon Load (%): 16 detection Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: 100% USP Listing: L1/L43 Bimodal phase combines C18 and PFP functional groups to increase selectivity in the separation of complex mixtures when compared to standard C18 phases alone. • Hydrophobic retention similar to C18 • PFP functionality provides alternate selectivity required for challenging applications Dimensions 1.9 μm ACME F5/C18 - 120 Å 5 μm Length (mm) x ID ACMF5C18-1.9-03021 3 μm ACMF5C18-5-03021 2.1 x 30 ACMF5C18-1.9-03046 ACMF5C18-5-03046 4.6 x 30 ACMF5C18-1.9-05021 ACMF5C18-3-03021 ACMF5C18-5-05021 2.1 x 50 ACMF5C18-1.9-05030 ACMF5C18-3-03046 ACMF5C18-5-05030 3.0 x 50 ACMF5C18-1.9-05046 ACMF5C18-3-05021 ACMF5C18-5-05046 4.6 x 50 ACMF5C18-1.9-10021 ACMF5C18-3-05030 ACMF5C18-5-10021 2.1 x 100 ACMF5C18-1.9-10030 ACMF5C18-3-05046 ACMF5C18-5-10030 3.0 x 100 ACMF5C18-1.9-10046 ACMF5C18-3-10021 ACMF5C18-5-10046 4.6 x 100 ACMF5C18-1.9-15021 ACMF5C18-3-10030 ACMF5C18-5-15021 2.1 x 150 ACMF5C18-3-10046 ACMF5C18-5-15030 3.0 x 150 - ACMF5C18-3-15021 ACMF5C18-5-15046 4.6 x 150 - ACMF5C18-3-15030 ACMF5C18-5-25046 4.6 x 250 - ACMF5C18-3-15046 - ACME F5/C18 - 120 Å Guard Columns Dimensions 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder ID x Length (mm) 2.1 x 5 ACMGC19UC521F5C18 ACMGC3LC521F5C18 ACMGC5LC521F5C18 3.0/4.6 x 5 ACMGC5LC546F5C18 2.1 x 10 ACMGC19UC546F5C18 ACMGC3LC546F5C18 ACMGC5LC1021F5C18 3.0/4.6 x 10 ACMGC5LC1046F5C18 ACMGC19UC1021F5C18 ACMGC3LC1021F5C18 ACMGC19UC1046F5C18 ACMGC3LC1046F5C18 Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-10 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Selectivity for Geometric Isomers Standard C18, 3µm HPLC/UHPLC Columns HPLC/UHPLC Columns 100 x 4.6 mm ACME F5/C18 F5/C18, 3µm Peaks 3 and 5 3 μm, 100 x 4.6 mm 100 x 4.6 mm unresolved Mobile Phase: Methanol:Water, (40:60, v/v) 3 45 Flow rate: 1.5 mL/min Temperature: 22 °C Detection: UV@225 nm Injection Vol.: 3 μL Sample: 1. Void peak 2. Impurity peak 3. 1,2,4-trimethoxybenzene 4. 1,2-dimethoxybenzene 5. 1, 2,3-trimethoxybenze Phase Selectivity C18 vs ACME F5/C18 ACME F5/C18, 3 µm, 100 x 4.6 mm Flow rate: 1.5 mL/min 7 ACME PLUS, 3 µm, 100 x 4.6 mm Temperature: 25 °C Mobile Phase: Detection: UV@245 nm ACME PLUS A: 25 mM Ammonium Formate in water Injection Vol.: 3 µL B: Acetonitrile Gradient: Standard C18, 3µm 100 x 4.6 mm 3 2 5 Profile 13 AB Time (min) 8 10 12 9 11 1 4 6 0 100 0 14 10 35 65 11 10 90 15 10 90 7 F5/C18, 3µm 100 x 4.6 mm Sample: 8. Verapamil ACME F5C18 1. Uracil 9. Propranolol 2. Adenine 10. Oxazepam 13 3. Atenolol 11. Fentanyl 4. Pindolol 12. Tolbutamide 3 5 5. Codeine 13. Diazepam 6. Norfentanyl 14. D9-Tetrahydrocannabinol 2 10 7. Acebutolol 1 8 9 12 6 4 14 11 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-11 LIFE SCIENCE

Colonnes HPLC/UHPLC ACME Amide/C18 Colonnes HPLC/UHPLC Physical Properties SBeopnadreadtioPnhaMseo:d–e(:CRHe2v)3eNrHse(dC-=POh)a-Cse15H37 Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 260 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 120 Å Carbon Load (%): 19 Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: 100% USP Listing: L1/L60 Bimodal phase combines C18 and an embedded Amide • Ultra-high purity (99.999%) extra-treated porous spherical functional group to increase selectivity in the separation of silica, for excellent peak shape complex mixtures when compared to standard C18 phases alone. and reproducibility • Hydrophobic retention similar to C18 • Ultra-low bleed phase is suitable for use with UV and MS • Amide functionality provides alternate selectivity required detection for challenging applications • Similar retention window with ACME C18 • Amide/C18 can operate in 100% aqueous conditions and shows improved retention of polar compounds. Dimensions 1.9 μm ACME Amide/C18 - 120 Å 5 μm Length (mm) x ID ACMAM-1.9-03021 3 μm ACMAM-5-03021 2.1 x 30 ACMAM-1.9-03046 ACMAM-5-03046 4.6 x 30 ACMAM-1.9-05021 ACMAM-3-03021 ACMAM-5-05021 2.1 x 50 ACMAM-1.9-05030 ACMAM-3-03046 ACMAM-5-05030 3.0 x 50 ACMAM-1.9-05046 ACMAM-3-05021 ACMAM-5-05046 4.6 x 50 ACMAM-1.9-10021 ACMAM-3-05030 ACMAM-5-10021 2.1 x 100 ACMAM-1.9-10030 ACMAM-3-05046 ACMAM-5-10030 3.0 x 100 ACMAM-1.9-10046 ACMAM-3-10021 ACMAM-5-10046 4.6 x 100 ACMAM-1.9-15021 ACMAM-3-10030 ACMAM-5-15021 2.1 x 150 ACMAM-3-10046 ACMAM-5-15030 3.0 x 150 - ACMAM-3-15021 ACMAM-5-15046 4.6 x 150 - ACMAM-3-15030 ACMAM-5-25046 4.6 x 250 - ACMAM-3-15046 - ACME Amide/C18 - 120 Å Guard Columns Dimensions 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder ID x Length (mm) 2.1 x 5 ACMGC19UC521AM ACMGC3LC521AM ACMGC5LC521AM 3.0/4.6 x 5 ACMGC5LC546AM 2.1 x 10 ACMGC19UC546AM ACMGC3LC546AM ACMGC5LC1021AM 3.0/4.6 x 10 ACMGC5LC1046AM ACMGC19UC1021AM ACMGC3LC1021AM ACMGC19UC1046AM ACMGC3LC1046AM Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-12 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Column Type Phase Selectivity C18 vs Amide/C18 ACME Amide/C18 C18 Phase 2 3 Phenols and Carboxylic acids HPLC/UHPLC Columns 3 μm, 100 x 2.1 mm 1 Isocratic Mobile Phase: 8 ACME C18 In the file you sent you have th Acetonitrile:Water:Formic Acid, 46 listed as a polar embedded. Pl (30:70:0.1, v/v/v) it as ACME Amide Flow rate: 1.0 mL/min 5 Temperature: 325 °C 7 9 Detection: UV@254 nm Injection Vol.: 3 μL Sample: 3 ACME Amide/C18 1. Void marker 2 2. Resorcinol 3. 4-Methoxyphenol ACME Amide 4 6 8 4. Phenol 5 7 9 5. 4-Methoxybenzoic Acid 1 6. 4-Methylphenol 7. 4-Methylbenzoic Acid 8. 4-Nitrophenol 9. 3,4-Dimethylphenol Tricyclic Antidepressants Hydrophobic Basic Pharmaceuticals 2 1 ACME Amide/C18 3 μm, 100 x 4.6 mm Sample: 3 20mM Ammonium Phosphate (Monobasic) 1. Nordoxepin 4 Flow rate: 1.0 mL/min 2. Desipramine Temperature: 30 °C 3. Doxepin Detection: UV@254 nm 4. Imipramine Injection Vol.: 5 μL Amide shows incredibly good peak shape Amide ACME Amide/C18 on hydrophobic basic pharmaceuticals. mine / Fluoxetine 1 ACME Amide/C18 3 Sample: 1. Diphenhydramine 2. Nor-Fluoxetine 3. Fluoxetine 2 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-13 LIFE SCIENCE

Colonnes HPLC/UHPLC Colonnes HPLC/UHPLC ACME PLUS Physical Properties SBeopnadreadtioPnhaMseo:dCe1:8HRe37versed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 200 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 200 Å Carbon Load (%): 12 Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: 100% USP Listing: L1 The ACME PLUS is a truly unique HPLC phase. It is made on a 200 Å silica using precision bonding and end capping. The large pore structure allows for: • Facile separation of proteins and peptides • 100% aqueous mobile phase compatibility with good retention for analysis of small molecules • Faster separations on many standard analyses Dimensions 1.9 μm ACME PLUS - 200 Å 5 μm ID x Length (mm) ACMP-1.9-03021 3 μm ACMP-5-03021 2.1 x 30 ACMP-1.9-03046 ACMP-5-03046 4.6 x 30 ACMP-1.9-05021 ACMP-3-03021 ACMP-5-05021 2.1 x 50 ACMP-1.9-05030 ACMP-3-03046 ACMP-5-05030 3.0 x 50 ACMP-1.9-05046 ACMP-3-05021 ACMP-5-05046 4.6 x 50 ACMP-1.9-10021 ACMP-3-05030 ACMP-5-10021 2.1 x 100 ACMP-1.9-10030 ACMP-3-05046 ACMP-5-10030 3.0 x 100 ACMP-1.9-10046 ACMP-3-10021 ACMP-5-10046 4.6 x 100 ACMP-1.9-15021 ACMP-3-10030 ACMP-5-15021 2.1 x 150 ACMP-3-10046 ACMP-5-15030 3.0 x 150 - ACMP-3-15021 ACMP-5-15046 4.6 x 150 - ACMP-3-15030 ACMP-5-25046 4.6 x 250 - ACMP-3-15046 Dimensions - ID x Length (mm) ACME PLUS - 200 Å Guard Columns 2.1 x 5 3.0/4.6 x 5 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges 2.1 x 10 *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder 3.0/4.6 x 10 ACMGC19UC521P ACMGC3LC521P ACMGC5LC521P ACMGC5LC546P ACMGC19UC546P ACMGC3LC546P ACMGC5LC1021P ACMGC5LC1046P ACMGC19UC1021P ACMGC3LC1021P ACMGC19UC1046P ACMGC3LC1046P Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-14 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Water Soluble Vitamins HPLC/UHPLC Columns ACME PLUS Sample: 6HPLC/UHPLC Columns 3 µm, 100 x 4.6 mm 1. Thiamine (Vitamin B1) 5 A: 50 mM KH2PO4 buffer (pH 3.0) 2. Pyridoxine (Vitamin B6) 4 B: Methanol 3. Niacinamide (Nicotinamide, Vitamin B3) 4. Folic Acid (Vitamin B9) Gradient: 5. Cyanocobalamin (Vitamin B12) 6. Riboflavin (Vitamin B2) Time Profile (min) AB 2 3 0 98 2 10 50 50 Flow rate: 1.0 mL/min Temperature: 30 °C Detection: UV@270 nm Injection Vol.: 10 µL 0 2.0 4.0 6.0 8.0 10.0 12.0 Time (min) Phenols 3 ACME PLUS 2 3 µm, 100 x 4.6 mm Isocratic Mobile Phase: 45 6 A: Water B: Acetonitrile 17 A:B, (75:25, v/v) Flow rate: 1.5 mL/min 89 Temperature: 25 °C Detection: UV@268 nm Injection Vol.: 1 µL Sample: 1. Uracil 2. Resorcinol 3. 4-Hydroxybenzaldehyde 4. Phenol 5. Methylparaben 6. p-Nitrophenol 7. p-Cresol 8. 3,4-Dimethylphenol 9. 2,6-Dimethylphenol 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN B-15 LIFE SCIENCE

Colonnes HPLC/UHPLCColonnes HPLC/UHPLC ACME C8 Physical Properties BSeopnadreadtioPnhaMseo:d-eC:8HRe17versed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 260 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 120 Å Carbon Load (%): 10 Endcapping: Yes proprietary pH Range: 1.8 to 9.5 Temperature Limit: 60 °C Aqueous Compatibility: ≤ 98% USP Listing: L7 Dimensions 1.9 μm ACME C8 - 120 Å 5 μm Length (mm) x ID ACMC8-1.9-03021 3 μm ACMC8-5-03021 2.1 x 30 ACMC8-1.9-03046 ACMC8-5-03046 4.6 x 30 ACMC8-1.9-05021 ACMC8-3-03021 ACMC8-5-05021 2.1 x 50 ACMC8-1.9-05030 ACMC8-3-03046 ACMC8-5-05030 3.0 x 50 ACMC8-1.9-05046 ACMC8-3-05021 ACMC8-5-05046 4.6 x 50 ACMC8-1.9-10021 ACMC8-3-05030 ACMC8-5-10021 2.1 x 100 ACMC8-1.9-10030 ACMC8-3-05046 ACMC8-5-10030 3.0 x 100 ACMC8-1.9-10046 ACMC8-3-10021 ACMC8-5-10046 4.6 x 100 ACMC8-1.9-15021 ACMC8-3-10030 ACMC8-5-15021 2.1 x 150 ACMC8-3-10046 ACMC8-5-15030 3.0 x 150 - ACMC8-3-15021 ACMC8-5-15046 4.6 x 150 - ACMC8-3-15030 ACMC8-5-25046 4.6 x 250 - ACMC8-3-15046 Dimensions - ID x Length (mm) ACME C8 - 120 Å - 120 Å Guard Columns 2.1 x 5 3.0/4.6 x 5 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges 2.1 x 10 *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder 3.0/4.6 x 10 ACMGC19UC521C8 ACMGC3LC521C8 ACMGC5LC521C8 ACMGC5LC546C8 ACMGC19UC546C8 ACMGC3LC546C8 ACMGC5LC1021C8 ACMGC5LC1046C8 ACMGC19UC1021C8 ACMGC3LC1021C8 ACMGC19UC1046C8 ACMGC3LC1046C8 Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-16 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Caffeine Metabolites 3 5 HPLC/UHPLC Columns 2 ACME C8 3 µm, 100 x 4.6 mm 1 Isocratic Mobile Phase: 4 A: 0.1% formic acid in water B: Methanol ACME C8 A:B, (80:20, v/v) Flow rate: 1.0 mL/min Temperature: 30 °C Detection: UV@270 nm Injection Vol.: 2 µL Sample: 3 5 1. Theobromine (3,7-Dimethylxanthine) 2 2. Paraxanthine (1,7-dimethylxanthine) 3. Theophylline (1,3-Dimethylxanthine) 1 4. 1,3-Dimethyl-7-(2-hydroxyethyl)xanthine 4 5. Caffeine (1,3,7-Trimethylxanthine) ACME C18 1 ACME C8 23 65% Methanol Tricyclic Antidepressants 4 6 ACME C8 5 3 µm, 100 x 4.6 mm Isocratic Mobile Phase: 1 ACME C18 A: 25 mM ammonium phosphate buffer 2 70% Methanol (pH 7.0) 3 B: Methanol 45 A:B, (35:65, v/v) Flow rate: 1.5 mL/min Temperature: 35 °C Detection: UV@254 nm Injection Vol.: 2 µL Sample: 1. Nordoxepin 2. Desipramine 3. Nortriptyline 4. Doxepin 5. Imipramine 6. Amitriptyline 6 1.0 2.0 3.0 4.0 5.0 6.0 7.0 Time (min) B-17 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN LIFE SCIENCE

Colonnes HPLC/UHPLCColonnes HPLC/UHPLC ACME CN (Cyano) Physical Properties Bonded Phase: -R-CN Separation Mode: Reversed-Phase Base Material: Ultrapure Extra-Treated Porous Spherical Silica Purity (%): 99.999, metals < 10 ppm Surface Area: 260 m2/g Particle Size (s): 1.9 µm, 3 µm, 5 µm Pore Size: 120 Å Carbon Load (%): 7 Endcapping: Yes proprietary pH Range: 2 to 7.5 Temperature Limit: 50 °C Aqueous Compatibility: 100% USP Listing: L10 Dimensions 1.9 μm ACME CN - 120 Å 5 μm Length (mm) x ID ACMCN-1.9-03021 3 μm ACMCN-5-03021 2.1 x 30 ACMCN-1.9-03046 ACMCN-5-03046 4.6 x 30 ACMCN-1.9-05021 ACMCN-3-03021 ACMCN-5-05021 2.1 x 50 ACMCN-1.9-05030 ACMCN-3-03046 ACMCN-5-05030 3.0 x 50 ACMCN-1.9-05046 ACMCN-3-05021 ACMCN-5-05046 4.6 x 50 ACMCN-1.9-10021 ACMCN-3-05030 ACMCN-5-10021 2.1 x 100 ACMCN-1.9-10030 ACMCN-3-05046 ACMCN-5-10030 3.0 x 100 ACMCN-1.9-10046 ACMCN-3-10021 ACMCN-5-10046 4.6 x 100 ACMCN-1.9-15021 ACMCN-3-10030 ACMCN-5-15021 2.1 x 150 ACMCN-3-10046 ACMCN-5-15030 3.0 x 150 - ACMCN-3-15021 ACMCN-5-15046 4.6 x 150 - ACMCN-3-15030 ACMCN-5-25046 4.6 x 250 - ACMCN-3-15046 Dimensions - ID x Length (mm) ACME CN - 120 Å - 120 Å Guard Columns 2.1 x 5 3.0/4.6 x 5 1.9 μm Cartridges 3 μm Cartridges 5 μm Cartridges 2.1 x 10 *use with UHPLC Holder *use with UHPLC Holder *use with UHPLC or HPLC Holder 3.0/4.6 x 10 ACMGC19UC521CN ACMGC3LC521CN ACMGC5LC521CN ACMGC5LC546CN ACMGC19UC546CN ACMGC3LC546CN ACMGC5LC1021CN ACMGC5LC1046CN ACMGC19UC1021CN ACMGC3LC1021CN ACMGC19UC1046CN ACMGC3LC1046CN Guard Column Holder1 (Peek Ferrules) Guard Column Holder2 (Stainless Steel Ferrules) Dimensions Holders Dimensions Holders 5 mm for 2.1 & 4.6 ID ACHOL-P5021/46 5 mm for 2.1 & 4.6 ID ACHOLS-5021/46 10 mm for 2.1 & 4.6 ID ACHOL-P10021/46 10 mm for 2.1 & 4.6 ID ACHOLS-10021/46 1 For HPLC 2 For High pressure UHPLC B-18 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE