HPLC Catalogue

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Table des Matiéres - Table of Contents About Canadian Life Science 2 Table des Matiéres - Table of Contents Seminars & Support 3 What’s New? 4, 5 History about Chromatography 6, 7 HPLC Terms, Conditions & Calculations 8 Column Selection Overview 9 Column Selection by Chromatographic Mode 10, 11 Selectivity vs Hydrophobicity 12 Column Selection by Trademark/Brand 13 - 18 Column Selection by USP Chromatographic Packing/USP Listing 19 - 29 Loading Capacity based on Column Parameters 30 - 31 Preparative Chromatography 32 Mobile Phase Preparation & Research Chemicals 33 Mobile Phase Solvent Properties 34-35 Column Protection 36, 37 Column Cleaning & Storage 38, 39 ACE® A ACME™ B List of formerly Grace Discovery HPLC Column Brands available C HALO® D InertSustain®/Inertsil® E NUCLEODUR®/NUCLEOSIL® F Shodex™ G PrimeSep®/Obelisc®/Sharc H Chiral Chromatography I Customer Service I-22 Mentioned trademarks are registered trademarks of their respective owners. No license or immunity under any patent is granted or implied by our sale of any material. Canadian Life Science is not affiliated with any of the following companies listed, or their products. Products listed under the following companies are compatible with their instrumentation. Agilent, Beckman, Bruker, CTC/Leap, Perkin Elmer, Phenomenex, Shimadzu, Waters. Canadian Life Science is not an authorized seller of AGILENT branded parts and does not sell genuine AGILENT parts. Canadian Life Science only offers parts compatible with Agilent products. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 1 LIFE SCIENCE

Services Techniques Services Techniques About Canadian Life Science / À propos de Canadian Life Science Chromatography Vials, Caps, Inserts At Canadian Life Science we understand that HPLC columns are a critical Columns & Supplies & 96-well plates tool in your analytical work; therefore we place paramount importance on helping you choose the right HPLC column for your application. We always strive to broaden our expertise and today offer a complete spectrum of solutions for your HPLC column needs. Most chromatographic applications can be solved through the right combination of HPLC column and analytical conditions. At times, one may encounter challenging compounds creating a need for HPLC column chemistry of different selectivity. We provide you with an excellent choice of products for small and large molecules ranging from traditional reversed-phase, fluorinated phase, mixed-mode, HILIC, ion-exchange, normal phase, size exclusion, ligand exchange and newly developed HPLC columns that can operate at extended pH ranges. We also offer a complete line of superficially-porous particle (Fused-Core®) core-shell type columns that can help you increase productivity without exceeding the pressure limits of traditional HPLC systems. Our column selection provides customers with a wide range of reproducible and robust phases. Discover the benefits of dealing with us by contacting us today to help solve your difficult separations. We hope that our HPLC and UHPLC column catalogue will be a useful tool for your column selection. Dissolution Honeywell Research We are introducing the first of a series of catalogues that will cover the Accessories Chemicals different product lines that we carry. All our products can also be found on our online store, www.lifescience.ca The CLS Team Chez Canadian Life Science nous comprenons que les colonnes HPLC ou 2D Sample Storage Biochromatography UHPLC sont des outils essentiels pour vos analyses. Nous accordons une grande importance à élargir notre expertise afin de vous aider à choisir la bonne colonne HPLC pour votre application et à vous offrir une gamme de colonnes la plus complète possible vous offrant un maximum de flexibilité pour répondre à vos besoins analytiques. La plupart des analyses chromatographiques peuvent être résolues par une bonne combinaison de chimie et de conditions analytiques. Certaines molécules ou matrices complexes requièrent l’expérimentation de sélectivités différentes ou de modes de séparations différents. Nous vous proposons donc un large inventaire de colonnes traditionnelles de phase inverse et de phases alternatives tel que les phases fluorées, à mode mixte, HILIC, à échange d’ions, à phase normale, à exclusion, et bien d’autres. Nous vous proposons également une gamme de colonnes à particules superficiellement poreuse (Fused-Core®) qui vous permettent d’augmenter la productivité tout en respectant les limites de pression des instruments HPLC traditionnels. Découvrez les avantages de faire affaires avec nous en nous contactant dès aujourd’hui pour vous aider à résoudre vos séparations difficiles. Nous espérons que notre catalogue de colonnes HPLC et UHPLC sera un outil utile pour votre sélection de colonnes. Nous vous présentons ici le premier catalogue d’une série de catalogues qui Liquid Handling Analytical couvriront nos différentes lignes de produits. Tous nos produits sont aussi & Robotics Standards accessibles sur notre boutique en ligne shop.lifescience.ca L’équipe CLS 2 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Seminars & Support Technical Services Application Support As an initial step in method development or method optimization we invite you to search our application database available on our website www.lifescience.ca Technical Support Get in touch with us to discuss method development strategies. Our technical support team is staffed with knowledgeable and experienced people who are available to discuss your needs. We are here to provide you optimized method development support. Please contact us by telephone or email as per below. Call: 1.888.226.2775 Email: [email protected] Free Column Screening and Try & Buy programs As an additional support to our application database and technical support, we offer free column screening services or Try and Buy programs to allow you to test columns before committing to buy. Please contact your technical sales representative to discuss our programs. Technical Seminars Our technical seminars and training courses are an excellent way of training your staff and keeping up to date with the latest chromatography developments. We offer off-site and on-site technical seminars and the content can be varied to meet your specific needs for on-site technical seminars and/or presentations. Webinars Throughout the year, our suppliers offer live webcast and recorded webinars via various platforms. Visit Linkedin.com/company/canadian-life-science for information on our upcoming technical training seminars, new product releases, webinars and updates. Be sure to visit lifescience.ca to sign up for our monthly newsletter and stay informed! 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 3 LIFE SCIENCE

ServQicueois dTechNeniuqfuesQuoi de Neuf ACE® HILIC Method Development kits The ACE® HILIC MDK provides 3 HILIC column chemistries for polar retention specifically designed to provide alternative selectivity to each other in HILIC mode (HILIC-A, HILIC-B, HILIC-N) Ask your Technical Sales Rep for a copy of the systematic approach to HILIC Method Development Guide ACE® HILIC-A (phase with acidic character) ACE® HILIC-B (phase with basic character) ACE® HILIC-N (phase with neutral character) More details on pages A-30 - A-39 TM InertSustain™ AQ-C18 Shodex™ introduced the family of Daicel CHIRALPAK® chiral immobilized GL Sciences continues to expand the HILICpak® HPLC columns offering columns now available in sub-2 μm robust InertSustain HPLC and UHPLC a wide selection of polymeric based for normal phase and reversed- column product line by adding the HILIC phases. phase UHPLC and SFC separations. InertSustain™ AQ-C18 to the family. HILICpak® VG (Amino), HILICpak® VT CHIRALPAK® IA-U, CHIRALPAK® IB-U, InertSustain™ AQ-C18 columns are (Quaternary Ammonium), HILICpak® VC CHIRALPAK® IC-U, CHIRALPAK® ID-U, designed to achieve strong retention of (Carboxyl) and HILICpak® VN (Diol). CHIRALPAK® IG-U, CHIRALPAK® IH-U. highly polar compounds. The bonding More details on page I-14 optimization of the C18 groups at More details on pages G-4 - G-9 equal distance to the silica gel enables InertSustain™ AQ-C18 to offer significant retention for highly polar compounds even under water rich mobile phases. More details on pages E-10 - E11 4 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

• stimulants e.g. amphetamine • structural isomers e.g. methoxy- isomers • opiates What’s New? • opioids • unsaturated compounds The F5/C18 combines octadecyl (C18) and pentafluorophenyl (PFP) functionalities to pro novel proprietary phase that maximizes the properties of these two-phase chemistries. T result is a hybrid phase that is very orthogonal to a standard C18. The retention window What’s New? matches the standard C18 very well, so during method development, exchanging column without changing mAoCbMileE p™hFa5s/eC c1o8nditions will rapidly provide an alternate selectivity fo ACME™ Amide/C18 compounds of interest. C18 + PFP = F5/C18 wCsTth1ahe8nicdAvheamirrmdyidpwCea1/erC8ltl1.s, 8Tsaohlcteoednrurnetraatineitnegnstmsiaoenelnetehcwmtoiivndbidtedyodewwdveehmdleoanpptomcchloaeemrnsgtpt,rahoerexuTcpleicofphdshhetmea(atatE soinnFpmPedg5aoG iaec/un)orCsngd m1sd8imsp aoaillsnsaiodtri otffouon nrss cT(pc.tuP hhahtTsFieenahoePmsde nF)iean 5sifpsutr / taghrdCnrosrai 1ceC aptat8sd1irm .o1ici8eeTnoa0.thna mx0aelti%imr btmyrii eei nazmesseequttssauhlotntoeohpiucosdertfusaoaap sddtch rhetucoyuacocbpyretmrei linbrad(ptgCeinape 1gpoths8iirnvab)ooe slaafcelenttep hCtdsvrhe1osepas 8proteeyr fnfii t soelttwoathvraw ofeeslr-rue ypyFooph5rraga/orCaspaen1hti8ieco n pnoyh rlo anfs ohe i ogshrhgolyaw npsioc columns without changing mobile phase conditions will rapidly orthogonal to a standard C18. ACME™ F5/C18 is the column provide an alternate selectivity for many compounds of choice when a standard C18 column does not provide of interest. adequate resolution. More details on pages B-12 - B-13 More details on pages B-10 - B-11 HASLMOA®LLC3M0OLECULE • Highly reprodHuAcSiLbMOleA®bLBLoinMpdhOeedLnEpyChlUaLseE rugged separations • 100 % Aqueous Compatibility Best Applications: 90 Å 2 micron particle 90 Å 2.7 micron particle •90 Å 5 micron particle Fat/Water Soluble90VÅi2tmaimcroninpasrticle• Lipids 90 Å 2.7 micron particle 90 Å 5 micron particle • Carotenoids • Steroids BIOCLASS BIOCLASS HALO® Fused-Core® introduced the first superficially porous The HALO® Biphenyl offers a new perspective on retention C30 phase bringing resolution and efficiency together which mechanisms for polar compounds. With a combination of makes it an ideal choice for the separation of carotenoids, hydrophobic, aromatic, and polar selectivities, the HALO® fat/water soluble vitamins, lipids and steroids. Biphenyl, joined with the efficiency of robust Fused-Core® tecPhEnAoKlIoDgEyN,TuITnIElSocks powerful separation forces. Experience the difference one phase can make!121..60DRÅee2tlitmnaiyctrlooanccpoeaprttaihctleeero(Al )(E) Mo16r0eÅ details on2 micron particle pages D-22 - D-23160 Å 2.7 micron particle 160 Å 5 micron particle 160 Å 2.7 micron particle 160 Å 5 micron particle PEPTIDE 3. Ergocalciferol (D2) PEPTIDE 4. Cholecalciferol (D3) Mo56r.. eDAlLdp-haelaptthoaac-iotlopschoeoprohnel (rEpo)laacgeetatDe (-E1) 6 7. 2,3-trans-phylloquinone (K) 8. Retinyl palmitate (A) 1000 Å 2.7 micron particle 400 Å 3.4 micron particle 90 Å 2.7 micron particle COLUMN 90 Å 2.7 micron particle HALO 160 Å C30, 2.7 µm, 4.6 x 150 mm PROTEIN GLYCAN GLYCAN 1*0F0o0rÅm2.e7tmhiocrdoncpoarntidcleitions please s4e0e0 fÅu3l.l4 micron particle application at advancPeRdO-mTEaItNerials-tech.com 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 5 LIFE SCIENCE

Services TechniquesServices Techniques A little bit of history about chromatography! M.S. Tswett (alternate spellings Tsvett, Tswet, Tsvet, Zwet, and Cvet) (14 May 1872, Asti, Italy – 26 June 1919, Voronezh, Russia) Inventor of adsorption chromatography. M.S. Tswett’s research led to the development of a special adsorption technique that permitted the separation of the leaf pigments. In later years, he further refined this technique, which eventually became known as chromatography or “color writing” from the Greek roots “chroma” for color and graphein” to write. Martin (Left photo) & Synge (Right photo) Archer John Porter Martin (1 March 1910, London, United Kingdom – 28 July 2002, Llangarron, United Kingdom) Richard Laurence Millington Synge (28 October 1914, Liverpool, United Kingdom – 18 August 1994, Norwich, United Kingdom) • R eceived Nobel prize (1952) for work in the Invention of Partition chromatography (1941) Key work: J.P. Martin and R.L.M. Synge, Biochem. J. 35, 1358, 1368 (1941) 1872 1910/1914 1925 1930 Joseph Jack Kirkland (24 May 24 1925, Winter Garden, Florida – 30 October 2018, Wilmington, Delaware) • Inventor of superficially-porous particles • Direct synthesis of narrow-size-range silica particles without particle sizing • Development of high purity silica particles – Type “B” silica • Development of low-pH packings that were stable at higher temperature Csaba Horváth (25 January 1930, Szolnok, Hungary – 13 April 2004, New Haven, Connecticut) • First scientist to design, construct and show molecular separations using high-pressure liquid chromatography (HPLC) (1964 - 1966) and commercialized in 1968 by Picker Nuclear Company • Development of the solvophobic theory of reversed-phase HPLC • Developed support-coated open tubular (SCOT) columns 6 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

Technical Services Lloyd Robert Snyder Technical Services (30 July 1931, Sacramento, California – 20 September 2018, Walnut Creek, California) • Retention in liquid-solid chromatography • Classification of mobile phases and solvent selectivity • Linear solvent strength gradient elution theory • Column selectivity via hydrophobic subtraction theory Nobuo Tanaka (30 November 1945, Japan – ) • P rofessor Tanaka’s research has been focused on the development and characterization of high-performance columns and stationary phases (Tanaka protocol) for HPLC • Development of ultra-high efficiency columns based on monolithic silica gel and has been successful in realizing 1,000,000 theoretical plates even for retained solutes with long monolithic silica capillary columns • D evelopment of the monolithic silica rod column which was commercialized by Merck in 2000. • Major contributor in the area of monolithic silica capillary columns for LC and CEC 1931 1945 1948 199478 Uwe-Dieter Neue (born 1948 in Neunkirchen (Saar) Germany – 3 December 2010, Ashland (formerly Framingham), Massachusetts) • E xpert in liquid chromatography, stationary phase development, column-packing technology, and numerous applications • C oinventor of invented Radial-PakTM Cartridges and the Radial Compression Separation System for analytical HPLC • Invented the polar embedded carbamate group with C18, SymmetryShieldTM RP18 columns • Coinventor of the passive column pre-heater for UHPLC Founded in 1997, Canadian Life Science is dedicated to supplying the highest quality CANADIAN columns and consumable products to the chromatography industry. LIFE SCIENCE As Canada’s leading chromatography column and accessory supplier, we have gained an unrivalled reputation for excellent quality, competitively priced products and fast delivery, all backed up with expert technical support and after-sales service. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 7 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC HPLC Terms & Definitions Peak Asymmetry vs. Tailing Factor Guide pour la Sélection de Colonnes HPLC/UHPLC HPLC Term Symbol Definition Asymmetry Factor, As Tailing Factor, Tf Volume of column not occupied by column IUPAC, ASTM USP Void volume V0 Void time t0 packing Measured at 10% peak height Measured at 5% peak height System Delay Volume VD Retention time of an unretained peak 1.0 1.0 Retention time tR 1.3 1.2 Retention factor k’ Volume from solvent mixing to the column 1.6 1.4 1.9 1.6 Separation factor α Retention time of a retained peak 2.2 1.8 2.5 2.0 k’ = (tR –to)/to 3.0 2.3 α = k’2/k’1 (band spacing) 4.0 3.0 N = 5.54 (tR /w0.5)2 Aim to keep Tf < 2.0 or As < 2.5 Efficiency N (w0.5 = peak width at half-height) Resolution, Rs Resolution, Rs The fundamental resolution equation allows peak resolution (aRnsd) to be expressed in terms of retention (k’), selectivity (α) efficiency (N) is: − = 1.18 ∗ +. Rs = 1.18*(6.0 - 4.5)/(0.20 + 0.24) . = 1.18*(1.5)/0.44 = 4.0 tR1 tR2 (N) is: Selectivity Term W0.5 peak 1 W0.5 peak 2 Retention Term = 0.20 min Efficiency Term = 0.24 min = ∗( − 1) ∗ ( ) Peak 1/2 height () + 1) 4 ( ßW1à ßW2à Peak Height vs. Retention (k’) Different Approaches to Increasing Resolution* = 4 ∗( − 1) ∗ ( () () + 1) 1.000 k’/(k’+1) 25 Runtime 20 Parameter Increase N by 4x Increase k’ by 4x Increase by 12% 0.800 N k' 10000 40000 10000 10000 10000 10000 R 15 Runtime, min How? 22 28 22 Peak Height 0.600 10 1.10 1.10 1.10 1.10 1.10 1.23 0.400 1.55 3.03 1.55 2.02 1.55 3.0 Change L ∆ Mobile Phase (%B, Type) Peak Height Change dp ∆ Stationary Phase 0.200 5 ∆ Mobile Phase (%B) 0.000 0 0 2 4 6 8 10 12 14 16 18 20 Retention, k' 8 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide HPLC/UHPLC Column Selection Overview MW Solvent Solubility Solvent Separation Mode Column Phase Recommended Brands Bare silica ACME, Inertsil Hexane Soluble Normal Phase - Adsorption HPLC/UHPLC Column Selection Guide Normal Phase - Absorption - Diol, Cyano (CN), Amino (NH2) ACE, ACME, InertSustain, HALO Organic C18, C8, C4, PhenylHexyl, Phenyl (Ph), ACE, ACME, InertSustain, HALO Soluble Bonded Phase ACE, ACME, InertSustain, HALO Reversed Phase Cyano (CN), Amino (NH2) MeOHS,oMluebOleH-H20 Hydrophilic Interaction Amide, Diol, Phenyl (Ph), Cyano (CN), Inertsil, Jordi, Shodex Chromatography (HILIC) ACE, ACME, InertSustain, HALO Amino (NH2) MW<5000 THF Soluble Gel Permeation Chromatography (GPC) Small pore Silica, PS-DVB or DVB ACE, ACME, InertSustain, HALO Inertsil AX, Inertsil CX Aqueous Non-Ionic Reversed Phase C18, C8, C4, Ph, CN, NH2 Soluble Ionic ACE, ACME, InertSustain, HALO Reversed Phase, Ion-Pairing, C18, C8, C4, Ph, CN, NH2 Ion-Suppression AX, CX Ion Exchange Peptides, Proteins Reversed Phase C18, C8, C4, Ph, CN, NH2 Organic Gel Permeation Chromatography (GPC) Large pore Silica, PS-DVB, DVB Inertsil WP300 Diol, Jordi, Shodex Soluble Gel Filtration Chromatography (GFC) Inertsil WP300 Diol, Jordi, Shodex MW>5000 Ion Exchange pH 2-7.5, large pore Silica, Aqueous PVA, PVMA, DVB Jordi, Shodex Soluble Shodex IEC SP-825, CM-825 pH > 7.5, large pore Silica, Shodex IEC QA-825, DEAE-825 PVA, PVMA, DVB Cation Exchange Anion Exchange pH 2-7.5, large pore Silica, PVA, PHMA, DVB Inertsil, Jordi, Shodex, HALO BioClass Reversed Phase pH > 7.5, large pore Silica, PVA, PVMA, DVB Inertsil, Jordi, Shodex, HALO BioClass Hydrophobic Interaction PHMA with Phenyl group or large pore Silica Inertsil WP300 C4, C8, C18 Shodex Chromatography (HIC) with C4, C8, C18 PH-814, HALO BioClass Affinity, Bioaffinity PHMA with affinity ligand Shodex AF Pak 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 9 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC Column Selection by Chromatographic Mode Brand Examples Reversed-Phase • ACE® • ACME™ • Most commonly used mode • InertSustain® • Eluent: methanol-water, acetonitrile-water • HALO® • Chemistry: C18, AQ-C18, C18/PFP, C18/ Amide, C8, C4, Phenylhexyl, Phenyl, PFP, Cyano, Amino, etc. Hydrophilic-Interaction (HILIC) Brand Examples • Used when RP retention is poor • ACE® • Eluent: water-methanol, water-acetonitrile • ACME™ • Chemistry: Bare Silica, Amide, Diol, PFP, • InertSustain® • HALO® Cyano, Amino, Polyhydroxy, Penta-hydroxy, • NUCLEODUR® Ammonium-Sulfonic acid, etc. Normal-Phase Brand Examples • Used when sample is insoluble in • ACE® methanol-water and soluble in a solvent • ACME™ such as hexane • InertSustain® • HALO® • Eluent: isopropanol-hexane • Chemistry: Bare silica, Diol, Cyano (CN), Amino (NH2) Ion-Exchange Brand Examples • Used for ionic samples soluble in • Inertsil® (AX, CX ) aqueous solutions • Shodex™ IEX (Axpak, • Eluent: aqueous buffer of varying ionic Cxpak, CM-825, strength QA-825, DEAE-825, SP-425, SP-420, • Chemistry: Benzenesulfonyl, Carboxymethyl, PIKESS SP, Diethylaminoethyl, Diethylaminopropyl, PIKESS DEAE) Quaternary Ammonium, Sulfopropyl, Sulfo(Na⁺) 10 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide Column Selection by Chromatographic Mode Brand Examples HPLC/UHPLC Column Selection Guide Size Exclusion • Jordi Resolve • Shodex™ OHpak • Separation driven by Molecular Size • Shodex™ Asahipak • Mobile phase serves as a carrier • Eluent: water or organic solvent (GS-HG, GF-HQ) • Base material: Divinylbenzene, • Shodex™ MSpak • Shodex™ PROTEIN (KW, LW) Polyhydroxymethacrylate, Polyvinyl alcohol, • Shodex™ GPC Silica, Styrene-divinylbenzene copolymer M+ = Metal Ion Ligand-Exchange Brand Examples • Separation Mode based on a compound’s • Shodex™ SUGAR ability to form labile complexes with transition (SC, SP0810, KS-800 metal cations immobilized on a cation- SZ5532, SC1011, exchange stationary phase SC1211) • Analyte retention is increases with the • Shodex™ Rspak DC-613 strength of the complex formed with the • Shodex™ USPpak metal ion MN-431 • Ideal for sugar separations Hydrophobic-Interaction Brand Examples • Separation Mode based on hydrophobic effect • ACE® • Eluent: aqueous buffer of varying ionic strength • ACME™ • Chemistry: Phenyl (Ph), Octyl (C8), • HALO® • Inertsil® Butyl (C4) • Shodex™ HIC PH-814 • Ideal separation technique for purifying proteins but maintaining biological activity due to low denaturing separation conditions Chiral Interaction Brand Examples • Chiral chromatography based on the separation • CHIRALPAK® of racemic compounds into their enantiomers • CHIRALCEL® • CROWNPAK® • The enantiomer that forms the more stable • NUCLEODEX association with the chiral stationary phase will • Shodex™ ORpak be the more strongly retained species of the • WHELK® racemate. • Chiral phases: polysaccharide-based, cyclodextrin-based, protein-based (AGP/CBH/HSA) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 11 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLCSelectivity vs Hydrophobicity Polarity The performance of reversed-phase materials depends on many parameters. Two key properties, hydrophobicity and polarity, are of practical importance and dominate their selection. Halo RP-Amide ACME BIO2 ACME PAH InertSustain C18 Inertsil ODS-EP ACME BIO3 ACE 5 C18-HL Halo 5 C18 Inertsil CN-3 ACME PLUS ACME C8 Inertsil ODS-2 HALO AQ-C18 Halo C18 Excel C18-Amide Ultracore SuperC18 Halo C8 Inertsil ODS-3 ACE 5 C18-300 ACE 5 C18 ACE 5 C8 Halo 5 C8 Excel SuperC18 ACE 5 C8-300 Inertsil C8-3 ACE 5 C4-300 Excel CN-ES InertSustain C8 ACME C18 Inertsil WP300 C18 ACE 5 C4 InertSustainSwift C18 Inertsil C8-4 Inertsil WP300 C8 Inertsil ODS-4 Inertsil ODS-SP Inertsil ODS-P Ace 5 C18-PFP ACME F5/C18 InertSustain Phenylhexyl ACE 5 AQ ACE 5 C18-AR ACME Amide/C18 InertSustain AQ-C18 ACE 5 CN-300 HALO Peptide ES-C18 UltraCore SuperPhenylHexyl HALO Biphenyl ACME CN Halo Phenyl-Hexyl ACE 5 CN ACE Phenyl-300 Halo 5 Phenyl-Hexyl HALO C30 Halo 5 ES-CN HALO PFP Halo ES-CN ACME Biphenyl Halo 5 PFP InertSustain Phenyl ACE 5 Phenyl ACME Ph Inertsil Ph-3 Hydrophobicity Hydrophobicity The strength of hydrophobic interaction can be measured by the retention of neutral (non-polar) molecules. The percentage of carbon in the material is a simplistic but useful guide to the retention characteristics of the column. In the example above this loose correlation is demonstrated by the increase in retention observed when alkyl chain length (ie. carbon load) is increased. This results in an increase in hydrophobicity of the stationary phase. 12 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide Column Selection by Trademark/Brand Brand Manufacturer Base Material Phases/Functional Group Particle Sizes Page (µm) # ACE® 100Å Advanced Chromatography mesoporous silica ACE® 300Å Technologies Ltd mesoporous silica C18, C18-AR, C18-PFP, C18-Amide, AQ, C8, C4, Phenyl, CN, CN-ES, 1.7, 2, 3, 5, 10, 15 A8 HPLC/UHPLC Column Selection Guide ACE® 90Å mesoporous silica Silica, HILIC-A, HILIC-B, HILIC-N ACE® UltraCore™ Advanced Chromatography Technologies Ltd pellicular silica C18, C8, C4, Phenyl, CN 3, 5, 10 Advanced Chromatography SuperC18 2, 3, 5, 10 A-16 Technologies Ltd SuperC18, Phenyl-Hexyl C18, F5/C18, Amide/C18, Biphenyl, C8, CN, Phenyl, Silica 2.5, 5 Please Advanced Chromatography enquire Technologies Ltd ACME™ Phase Analytical Technology mesoporous silica 1.9, 3, 5, 10 B-6 ACME™ PLUS Phase Analytical Technology mesoporous silica C18, PAH 1.9, 3, 5 B-14 ACME™ Ultimate Phase Analytical Technology mesoporous silica C18, F5/C18, AMIDE/C18, PLUS Phase Analytical Technology mesoporous silica 1.9, 3, 5 check ACME™ BIO2 C18 website 1.9, 3, 5 B-1 ACME™ BIO3 Phase Analytical Technology mesoporous silica C18 1.9, 3, 5 B-1 C-1 Adsorbosphere™ Dr. Maisch GmbH mesoporous silica C18, C18-HS, C18-UHS, C8, C1, CN, NH2, PAH, SCX, SAX, PAC, Silica 3, 5, 10 C-1 (formely Grace/Alltech®) C-1 C-1 Allsep™ Anion Hichrom (formely Grace/Alltech®) methacrylate Quaternary Ammonium 7 C-1 Allsphere™ Dr. Maisch GmbH mesoporous silica ODS-1, ODS-2, C6, C1, Cyano, Amino, SCX, Silica 3, 5, 10 (formely Grace/Alltech®) Cation-exchange 10 sulphonated polystyrene- Alltech Anion Exclusion Hichrom (formely Grace/Alltech®) divinylbenzene Alltech Anion/S IC Hichrom (formely Grace/Alltech®) mesoporous silica Quaternary Ammonium 10 Alltech Carbohydrate Hichrom (formely Grace/Alltech®) sulphonated polystyrene Ca² 5 C-1 OA-1000, OA-2000, IOA-1000, IOA-2000 Alltech Organic Acid Hichrom (formely Grace/Alltech®) sulphonated polystyrene- C18, C18-EPS, C18-AQ, C18-Amide, C8, Cyano, Silica, HILIC 6.5, 8, 9 C-1 divinylbenzene Alltima ​ Hichrom (formely Grace/Alltech®) mesoporous silica 3, 5 C-1 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 13 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC Column Selection by Trademark/Brand Brand Manufacturer Base Material Phases/Functional Group Particle Sizes Page (µm) # Apex™  Hichrom (formely Grace/Alltech®) mesoporous silica ODS, Phenyl, Amino, Silica 5 C-1 Apollo™ Hichrom (formely Grace/Alltech®) mesoporous silica C18, C8, Phenyl, Silica 5 C-1 Brava™ Dr. Maisch GmbH mesoporous silica C18-BDS, C18-ODS, C8, C8-BDS, CN, CN-BDS, Phenyl, Amino, Silica 3, 5 C-1 Capcell (formely Grace/Alltech®) CHIRALCEL®  Polymer-coated ‘capsule type’ C18 MG III, C18 MG II, C18 MG, C18 AQ, C18 ACR, C8 DD, C18 UG, C8 3, 5 Please Shiseido silica UG, C1 UG, Phenyl UG, CN UG, NH2 UG, SCX UG, C18 AG, C8 AG, C18 enquire Daicel SG, C8 SG, MF Ph-I, MF C8, MF SCX mesoporous silica OD, OJ, OZ, OX 3, 5, 10, 20 I-15 CHIRALPAK®  Daicel mesoporous silica IA, IB, IC, ID, IE, IF, IG, IH, AD, AS, AY, AZ, AGP, CBH, HSA, QN-AX, 3, 5, 10, 20 1-11 Chromegabond® ES Industries mesoporous silica QD-AX 3, 5, 10 HELIX Chromatography 2.7 Please Coresep™ Nacalai Tesque C30, WR-C18, C8, C6, C4, C2, Phenyl, Diphenyl, Cyano, FluoroSep 3, 5, 15 enquire COSMOSIL Nacalai Tesque RP Phenyl, FluoroSep RP Octyl, LS, Amino, Diol, SCX, Alumina, Ethyl 5 COSMOSIL Please Pyridine, Pyridyl Amide, NO2, Amino Phenyl enquire pellicular silica 100, S, SB Please enquire mesoporous silica 3C18-EB, C18-MS-II, C18-AR-II, C18-PAQ, Cholester, PBr, πNAP, PYE, NPE, PFP, CN-MS, C22-AR-II, C8-MS, C4-MS, TMS-MS, PE-MS, SL-II, Please polymer base/crosslinkable enquire packings HILIC, NH2-MS Sugar-D CROWNPAK® Daicel mesoporous silica CR(+), CR(-) 5 I-18 Develosil® mesoporous silica 3, 5, 7, 10 Nomura Chemical mesoporous silica Si-30, Si-60, Si-100, ANIDIUS, TMS, TMS-UG, 300C4-HG, C8, C8-UG, 3, 5, 10 Please Econosphere™  300C8-HG, ODS, ODS-K, ODS-N, ODS-P, ODS-A, ODS-T, ODS-UG, enquire Dr. Maisch GmbH ODS-HG, ODS-MG, ODS-SR, 300-ODS-HG, PAHS, XG-C18M, HSR-C18, (formely Grace/Alltech®) C30-UG, RP-AQUEOUS, Combi-RP, RPFULLERENE, RP-AQUEOUS-AR, C-1 ERP20, XG-30M, PH, PH-UG, 100Diol, 300Diol, CN, CN-UG, NH2 C18, C8, Amino, Silica Genesis™​ Hichrom (formely Grace/Alltech®) mesoporous silica C18, C8, C8 E/C, AQ, Phenyl, CN, NH2, Silica 3, 4, 7 C-1 GreatSmart™ Dr. Maisch GmbH mesoporous silica RP18 3, 5 C-1 (formely Grace/Alltech®) HALO® BioClass Advance Material Technologies pellicular silica C4, ES-C18, ES-CN, Glycan, Diphenyl, Phenyl-Hexyl 2.7, 3.4, 5 D-24 HALO® Fused-Core® Advance Material Technologies pellicular silica C18, AQ-C18, RP-Amide, C8, Phenyl-Hexyl, ES-CN, PFP, HILIC, 2, 2.7, 5 D-2 Penta-HILIC, C30, Biphenyl 14 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide Column Selection by Trademark/Brand Brand Manufacturer Base Material Phases/Functional Group Particle Sizes Page (µm) # Hamilton Hamilton Company polystyrene-divinylbenzene PRP-X100, PRP-X110, RCX-10, RCX-30, PRP-X200, PRP-X400, PRP-X800, HC-40 Ca², HC-75 Ca², HC-75 H, HC-75 Pb² 5, 7, 10 Please HPLC/UHPLC Column Selection Guide enquire Hamilton Hamilton Company methacrylate polymer PRP-X500, PRP-X600 7 Please enquire Hichrom RPB Hichrom mesoporous silica C8/C18 multi-alkyl 3.5, 5, 10 Please enquire Hypersil Thermo Scientific mesoporous silica BDS C18, BDS C8, BDS Cyano, BDS Phenyl, ODS, ODS-2, MOS, SAS, 3, 5 Please CPS, Silica, SAX enquire GL SCIENCES HPLC COLUMN CATALOG InertCore® GL Sciences pellicular silica C18 2.4 Inertsil® GL Sciences mesoporous silica ODS, ODS-HL, ODS-2, ODS-3, ODS-3V, ODS-4, ODS-4V, ODS-SP, 2, 3, 4, 5, 6, 10, 15 E-30/E-55 Inertsil® Wide Pore GL Sciences mesoporous silica ODS-EP, C8, C8-3, C8-4, C4, Amide, HILIC, NH2, Diol, SIL-100A, SIL- 150A, AX, CX, Sulfa C18, Peptides C18, Acrolein C18 GL SCIENCES HPLC COLUMN CATALOG C18, C8, C4, SIL 3, 5 InertSustain® GL Sciences mesoporous silica C18, AQ-C18, C8, Phenyl, Phenylhexyl, Amide, NH2, Cyano, PFP 1.9, 2, 3, 5 E-8 InertSustainBio GL Sciences mesoporous silica C18 1.9, 3 E-24 GL SCIENCES HPLC COLUMN CATALOG InertSustainSwift® GL Sciences mesoporous silica C18, C8 1.9, 3, 5 E-12 ICSep AN-1 Transgenomic n/a E-16 PS-DVB Quaternary amine 5, 13 Jordi Resolve GPC Jordi Labs DVB, Xstream, Cation, Anion Please Kromasil Eka Chemicals polymer base/crosslinkable 3.5, 5, 10 enquire EMD Millipore packings C18, C8, C4, NH2, Silica 5, 10 LiChrosorb® EMD Millipore 5 Please LiChrospher® mesoporous silica enquire mesoporous silica RP-18, RP-8, NH2, Diol, Silica (100Å), Silica (60Å) Please mesoporous silica enquire 100RP-18, 100RP-18e, 100RP-8, 100RP-8e, 100NH2, 100DIOL, 60RP-select B, Silica (100Å), Silica (60Å) Please enquire Please enquire Macrosphere Dr. Maisch mesoporous silica bare silica, WAX, WCX, SCX, SAX 5, 7 C-1 NUCLEODEX® Macherey-Nagel mesoporous silica -PM, -OH 5 I-20 NUCLEODUR® Macherey-Nagel mesoporous silica 1.8, 3, 5 F-1 C18 Gravity, C18 Gravity-SB, C8 Gravity, C18 Isis, C18 Pyramid, PolarTec, Phenyl-Hexyl, N2, PFP, Sphinx RP, C18 HTec, C18 ec, C8 ec, HILIC, CN, CN-RP, NH2, NH2-RP, SiOH 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 15 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC Column Selection by Trademark/Brand Brand Manufacturer Base Material Phases/Functional Group Particle Sizes Page (µm) # NUCLEOGEL® GPC Macherey-Nagel polystyrene-divinylbenzene N.A 5, 10 Please enquire NUCLEOGEL® SUGAR Macherey-Nagel sulphonated PS/DVB ION 300 OA (H), SUGAR Ca, SUGAR Pb, SUGAR Na 10 Please NUCLEOSIL® Macherey-Nagel mesoporous silica 3, 5, 7, 10 enquire C18, C18 AB, C18 HD, C18 Nautilus, Protect I, C8 ec, C8, C8 HD, C4, C2, Phenyl, CN, OH, NH2, dimethylamino, SA (SCX), SB (SAX), SiOH, F-6 Chiral-1, Chiral-2, Chiral-3 Obelisc™ SIELC mesoporous silica Obelisc™ R (RP), Obelisc™ N (NP) 5, 10 H-2 Partisil® Hichrom (formely Whatman) mesoporous silica ODS, ODS2, ODS3, C8, PAC, SAX, SCA, Silica Please Partisphere® 5, 10 visit Hichrom (formely Whatman) mesoporous silica C18, C8, PAC, SAX, SCX, TAC-1 (PFP), WAX, Silica Pirkle website Please 5 visit website Regis (RStech Corporation Chiral phase covalently bonded Alpha-Burke 2, beta-Gem 1, DACH-DNB, Leucine, Phenylglycine, 5, 10 Please to silica Pirkle 1-J, Whelk-O 1, Whelk-O 2, ULMO, enquire Platinum™ Dr. Maisch GmbH mesoporous silica C18, EPS C18, C8, EPS C8, Phenyl, Cyano, Amino, Silica, SAX 1.5, 3, 5 C-1 (formely Grace/Alltech®) POLY LC POLY LC mesoporous silica POLYETHYL A, POLYGLYCOPLEX, POLYHYDROXYETHYL A, 3, 5, 12 Please POLYPROPYL A, POLYSULFOETHYL A, POLYWAX LP enquire Prevail™ Hichrom (formely Grace/Alltech®) mesoporous silica C18 Select, C18, C8, Phenyl, Cyano, Amino, Organic acid, Amide 3, 5 C-1 Primesep® SIELC mesoporous silica A, 100, 200, 500, B, B2, C, P 5, 10 H-1 Promix™ SIELC mesoporous silica ProntoSIL® Bischoff Chromatography mesoporous silica AP, MP, SP 5 Please Purospher EMD Millipore 3, 5, 10 enquire ReproSil® 70/100/300 Dr. Maisch GmbH silica C18-EPS, C18 AQ, C30, C18 SH, C8 SH, Phenyl, CN 2, 3, 5 ReproSil® 80 Dr. Maisch GmbH mesoporous silica RP-18, RP-18e, RP-18 HC, Purospher STAR RP-18e, Purospher 3, 5, 7, 10 Please ReproSil®-Gold Dr. Maisch GmbH mesoporous silica 3, 5, 10 enquire ReproSil®-Pur Dr. Maisch GmbH mesoporous silica STAR RP-8e, Purospher STAR Si, Purospher STAR NH2 3, 5, 10 mesoporous silica C18, C18-AB, ODS-A, C8, C4, CN, Phenyl, NH2, Diol 3, 5, 10 Please enquire ODS-1, ODS-2, C8, C1, Phenyl, Hexyl, NH2, Diol, CN, Si, SAX, SCX Please C18, C8, C4, C2 visit C18, AQ-C18, C8, C4, C1, Si, Phenyl, CN, Diol, NH2 website Please visit website Please visit website Please visit website 16 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide Column Selection by Trademark/Brand Brand Manufacturer Base Material Phases/Functional Group Particle Sizes Page (µm) # ReproSil®-Pur Basic Dr. Maisch GmbH mesoporous silica C18, C18-HD, C8 HPLC/UHPLC Column Selection Guide 1.9, 2.5, 3, 5, 7, 10 Please C18, NH2, CM, SCX visit Pentafluorphenyl-USP-L43, C8F13H4 website C18, C18-DE, C18-Aqua, C18-NE, C18-T, C8, C8-DE, C8-Aqua, ReproSil®-Saphir Dr. Maisch GmbH mesoporous silica C8-NE, C4-Aqua, CN, HILIC-P, HILIC-A, Asidosil-S, Asidosil-C 1.8, 2.2, 3, 5, 10 Please visit website ReproSil®-Fluosil  Dr. Maisch GmbH mesoporous silica 2.2, 3, 5 Please visit website Reprospher® Dr. Maisch GmbH mesoporous silica 1.8, 2, 2.5, 3, 5, 10 Please visit website SHARC™ SIELC mesoporous silica Proprietary, Hydrogen-bond Adsorption 3, 5, 10 H-2 Shodex™ AFpak Showa Denko polyhydroxymethacrylate Dextran Sulfate, Heparin, Protein A, Protein G, WGA, Choline oxydase, 18 G-34 Shodex™ Asahipak Showa Denko acetylcholine esterase Shodex™ Asahipak IEX Showa Denko HPLC Columns Shodex™ Asahipak SEC Showa Denko polyvinyl alcohol ODP, C8-P, C4P 4, 5, 9, 13 Capture the Essence Shodex™ CXpak Showa Denko 2018-2019 Shodex™ GPC KF Showa Denko polyvinyl alcohol ES-502N (Diethylaminoethyl), ES-502C (Carboxymethyl) 9 Shodex™ GPC LF Showa Denko HPLC Columns Showa Denko Capture the Essence Shodex™ HIC Showa Denko 2018-2019 Shodex™ HILICpak Showa Denko Showa Denko polyvinyl alcohol GS-HQ, GF-HQ 6, 7, 9, 13, 20 G-20 Shodex™ IC Showa Denko Shodex™ IEC styrene divnylbenzene copolymer Sulfo (Na+) 6 HPLC Columns Shodex™ MSpak Capture the Essence styrene divnylbenzene copolymer KF-401HQ, KF-402HQ, KF-402.5HQ, KF-403HQ, KF-404HQ, 3, 6, 7, 8, 10, 18 2018-2019 KF-405LHQ, KF-406LHQ, KF-601, KF-602, KF-602.5, KF-603, KF-604, KF-605, KF-606, KF-606M, KF-607, KF-801, KF-802, KF-802.5, KF-803, G-22 KF-803L, KF-804, KF-804L, KF-805, KF-805L, KF-806, KF-806M, KF-806L, KF-807, KF-807L styrene divnylbenzene copolymer LF-404, LF-604, LF-804 6 G-27 polyhydroxymethacrylate PH-814 (Phenyl) 10 HPLC Columns polyvinyl alcohol Amino, Carboxyl, Diol, Quaternary ammonium 5 Capture the Essence 2018-2019 G-4 styrene divnylbenzene copolymer Y-521, T-521 (Sulfo) 12 HPLC Columns polyhydroxymethacrylate 8, 12 Capture the Essence polyvinyl alcohol QA-825 (Quaternary Ammonium), DEAE-825, SP-825, 2018-2019 SP-420N (Sulfopropyl), CM-825 (Carboxymethyl) 5 HPLC Columns N.A Capture the Essence 2018-2019 HPLC Columns Capture the Essence 2018-2019 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 17 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC Column Selection by Trademark/Brand Brand Manufacturer Base Material Phases/Functional Group Particle Sizes Page (µm) # Shodex™ OHpak Showa Denko polyhydroxymethacrylate LB-803, LB-806M, SB-802 HQ, SB-802.5 HQ, SB-803 HQ, SB-804 HQ, 6, 8, 10, 13 G-19 SB-805 HQ, SB-806 HQ, SB-806M HQ Shodex™ PIKESS Showa Denko polyhydroxymethacrylate SP-2B (Sulfopropyl), DEAE-2B, DEAE3N-4T (Diethylaminoethyl) 2.5 HPLC Columns Shodex™ RSpak Showa Denko 10 Capture the Essence Shodex™ RSpak Showa Denko polyhydroxymethacrylate NN-414, NN-614, NN-814 (Sulfo) 5 2018-2019 Shodex™ RSpak Showa Denko 3.5, 6 Shodex™ RSpak Showa Denko polyvinyl alcohol JJ-50 (Quaternary Ammonium) 4, 6 HPLC Columns Shodex™ RSpak Showa Denko 10 Capture the Essence styrene divnylbenzene copolymer DS-413, DS-613, RP18-415 2018-2019 Polymethacrylate DE-213, DE-413, DE-413L, DE-613 HPLC Columns Capture the Essence polyhydroxymethacrylate DM-614 2018-2019 HPLC Columns Capture the Essence 2018-2019 HPLC Columns Capture the Essence 2018-2019 HPLC Columns Capture the Essence 2018-2019 Shodex™ SEC Showa Denko mesoporous silica KW-402.5, KW-403, KW-2002.5KW-802.5, KW-803, KW-804, KW- 3, 5, 7 G-18 PROTEIN KW 2002.5, KW-2003, KW-2004 Shodex™ SUGAR Series Showa Denko styrene divnylbenzene copolymer SC1011, SC1211, SC1821 (Sulfo Ca²+, Sulfo Pb²+, Sulfo Na²+, Sulfo 6, 7, 17, 30 G-9 Zn²+), SP0810 (Sulfo Pb²), KS-801, KS-802, KS-803, KS-804, KS-805, KS-806, KS-807 (Sulfo Na), SZ5532 (Sulfo Zn²) Ultrasphere® Hichrom (formely Beckman) mesoporous silica ODS, Octyl, Cyano, Ion Pair 3, 5 Please enquire VisionHT™ Dr. Maisch GmbH mesoporous silica C18 Classic, C18 Basic, C18 HighLoad, C18 Polar, 1.5, 3, 5, 10 C-1 (formely Grace/Alltech®) HILIC, Silica Vydac® Hichrom (formely Grace/Alltech®) mesoporous silica 218TP, 238TP, 208TP, 214TP, 219TP, 202TP, 201TP, 218MS, 238MS, 5, 10 C-1 208MS, 214MS, 219MS Vydac® Denali Hichrom (formely Grace/Alltech®) silica C18, Silica 3, 5, 10, 15, 20 C-1 VYDAC® Everest Hichrom (formely Grace/Alltech®) mesoporous silica 238EV 5, 10 C-1 mesoporous silica Pirkle-type chiral 5, 10 Whelk® Regis Technologies mesoporous silica 3.5, 5 Please polymethacrylate Sulfobetaine enquire ZIC®-HILIC EMD Millipore (formely Sequant) Sulfobetaine 5 Please ZIC®-pHILIC EMD Millipore (formely Sequant) enquire Please enquire 18 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide HPLC/UHPLC Column Selection Guide Looking for support with British Pharmacopeia (BP), European Pharmacopeia (EP), Japanese Pharmacopeia or any other Pharmacopeia? Give us a call at 1.888.226.2775 or email our technical help desk at [email protected] 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 19 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC USP Chromatographic Columns Listing Description Our Brand L1 Octadecyl silane (ODS or C18) chemically bonded to porous or non-porous silica or ceramic micro-particles, ACE C18, ACE Excel C18, ACE SuperC18, ACME C18, 1.5 to 10 µm in diameter, or a monolithic rod ACME F5/C18, ACME AMIDE/C18, ACME PLUS, HALO C18, HALO Peptide ES-C18, Inertsil (ODS, ODS-2, ODS-3, ODS-4, L2 Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid ODS-SP) Inertsil ODS-P, Inertsil ODS-HL InertSustain C18, spherical core, 30 to 50 µm in diameter InertSustain AQ-C18, InertSustainSwift C18 Grace Alltech Pellicular C18, ECONO PREP ODS L3 Porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod ACE Silica, ACME Silica, HALO HILIC, Inertsil SIL-100Å, Inertsil SIL-150Å, Inertsil WP300-SIL L4 Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm in diameter Grace Alltech Pellicular Silica, ECONO PREP SIL L5 Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm in diameter Please enquire L6 Strong cation-exchange packing-sulphonated fluorocarbon polymer coated on a solid spherical core, 30 to 50 µm Adsorbosphere XL SCX, Partisil SCX in diameter L7 Octylsilane (C8) chemically bonded to totally or superficially porous silica particles, 1.5 to 10 µm in diameter, ACE C8, ACE Excel C8, ACME C8, HALO C8, Inertsil (C8, C8-3, or a monolithic silica rod C8-4), InertSustain C8, InertSustainSwift C8 L8 An essentially monomolecular layer of aminopropylsilane (NH2) chemically bonded to totally porous silica gel Inertsil NH2, InertSustain NH2 support, 1.5 to 10 µm in diameter, or a monolithic silica rod. L9 Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, Inertsil CX, Partisil SCX, Partisphere SCX 3 to 10 µm in diameter L10 Nitrile groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod ACE CN, ACE Excel CN-ES, ACME CN, HALO ES-CN, HALO Peptide ES-CN, Inertsil CN-3, InertSustain Cyano ACE Phenyl, ACE Excel Phenyl, ACME Phenyl, HALO Biphenyl, L11 Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod HALO Diphenyl, HALO Phenyl-Hexyl, Inertsil (Ph, Ph-3), InertSustain Phenyl, InertSustain Phenylhexyl L12 A strong anion-exchange packing made by chemically bonding a quartenary amine to a solid silica spherical core, Pellicular SAX 30 to 50 µm in diameter L13 Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameter Adsorbosphere TMS, Adsorbosphere XL C1, Allsphere C1, APEX C1 L14 Silica gel having a chemicallly bonded strongly basic quaternary ammonium anion-exchange coating, 5 to 10 µm Adsorbosphere SAX, Adsorbosphere XL SAX, Allsphere SAX, in diameter Macrosphere 300 SAX, Inertsil AX, Nucleosil SB, Partisil SAX, Partisphere SAX, Platinum SAX, Vydac 3021C 20 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide USP Chromatographic Columns Listing Description Our Brand L15 Hexylsilane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter Allsphere C6, Chomegabond C6, Grom-Sil Hexyl-1 HPLC/UHPLC Column Selection Guide L16 Dimethylsilane chemically bonded to porous silica particles, 5 to 10 µm in diameter Adsorbil C2, Chromegabond C2, Nucleosil C2, Polygosil C2 Alltech OA-1000, Alltech OA-2000, Alltech IOA-1000, Alltech IOA- L17 Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the 2000, Hamilton HC-75H, Hamilton PRP-X200, Hamilton PRP-X300, hydrogen form, 6 to 12 µm in diameter Nucleogel Sugar 810H, Nucleogel ION 300 OA, Shodex IC Y-521, Shodex RSpak KC-811, Shodex SUGAR SH1011, Shodex SUGAR SH1821, Shodex SC1011, Shodex SC1211, Shodex SC1821 L18 Amino and cyano groups chemically bonded to porous silica particles, 3 to 10 µm in diameter Adsorbosphere PAC, Chromegabond A/CN, Legacy L18, Partisil 10 PAC, Partisphere PAC Hamilton HC-40,Hamilton HC-75 Ca, Nucleogel Sugar Ca, L19 Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the Nucleogel Sugar 810 Ca, Shodex SUGAR SC1011, calcium form, 5 to 15 µm in diameter Shodex SUGAR SC1211, Shodex SUGAR SC1821, Shodex USPpak MN-431 L20 Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10 µm in diameter, or a Inertsil Diol, Nucleosil Diol, Shodex PROTEIN KW-800 series, monolithic silica rod Shodex KW-400 series Nucleogel RP, Shodex RSpak DS-413, Shodex RSpak DS-613, L21 A rigid, spherical styrene-divinylbenzene copolymer, 3 to 30 µm in diameter Shodex RSpak RP18-413, Shodex RSpak RP18-415, Shodex RSpak RP18-613 Alltech Anion Exclusion, Nucleogel SCX, Shodex CXpak P-421S, Shodex IC Y-521, Shodex RSpak DC-613, Shodex RSpak KC-811, L22 A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, 5 to 15 µm in diameter Shodex SUGAR KS-800 series, Shodex SUGAR SC1011, Shodex SUGAR SC1211,Shodex SUGAR SC1821, Shodex SUGAR SP0810, Shodex SUGAR SZ5532, Shodex USPak MN-431 L23 An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quartenary ammonium groups, Allsep, Nucleogel SAX, Shodex IEC QA-825 7 to 12 µm in size L24 Polyvinylalcohol chemically bonded to porous silica particles, 5 µm in diameter Please enquire Packing having the capacity to separate compounds with a molecular weight range from 100-5000 Da(as deter- L25 mined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate Shodex OHpak SB-802HQ, Shodex OHpak SB-802.5HQ, resin base, cross-linked with polyhydroxylated ether (surface contained some residual carboxyl functional groups) Shodex SB402.5 was found suitable ACE C4-300, ACE C4, ACE Excel C4, HALO Protein C4, Inertsil L26 Butyl silane chemically bonded to totally porous or superficially porous silica particles, 1.5 to 10 µm in diameter C4, Inertsil WP300-C4, Vydac 214ATP, Vydac 214MS, Vydac 214TP, Vydac C4 L27 Porous silica particles, 30 to 50 µm in diameter Daisogel L28 A multifunctional support, which consists of a high purity, 60 Å, spherical silica substrate that has been bonded with Alltech mixed mode C8/anion, Legacy L28, ProTec C8 a cationic exchanger, sulfonic acid functionality in addition to a convention reversed phase C8 funcionality 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 21 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC USP Chromatographic Columns Listing Description Our Brand L29 Gamma alumina, reverse-phase, low carbon percentage by weight, alumina-based polybutadiene spherical par- Gammabond ARP-1, Gammabond Alumina Potency ticles, 5 µm in diameter with a pore volume of 80 Å L30 Ethyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter APEX Prepsil C2, Chromegabond C2-E, Nucleosil C2 A hydroxide selective, strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core Please enquire L31 of 8.5 µm macroporous particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with CHIRALPAK WH, Nucleosil Chiral-1 55% divinylbenzene L32 A chiral ligand-exchange resin packing-L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10 µm in diameter L33 Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spheri- Inertsil WP 300 Diol, Macrosphere 150Å, Shodex PROTEIN cal, silica-based, and processed to provide pH stability KW-800 series, Shodex KW-400 series L34 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead Nucleogel Sugar Pb, Shodex SUGAR SP0810 form, 7 to 9 µm in diameter L35 A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase Please enquire having a pore size of 150 Å L36 A 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5 µm aminopropyl silica L-Phenylglycine, Nucleosil Chiral-3 L37 Packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000 Da. It is a Shodex OHpak SB-803 HQ, Shodex SB403-4E polymethacrylate gel L38 A methacrylate-based size-exclusion packing for water-soluble samples Shodex SB400 series, Shodex OHpak SB-800HQ series L39 A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin Shodex SB400 series, Shodex OHpak SB-800HQ series, Shodex ODP2 HP, Shodex RSpak DM-614 L40 Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 3 to 20 µm in diameter CHIRALCEL OD, CHIRALCEL OD-H L41 Immobilized 1-acid glycoprotein on spherical silica particles, 5 µm in diameter CHIRALPAK AGP L42 Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5 µm in diameter Chromegabond PSC, HiChrom RPB 22 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide USP Chromatographic Columns Listing Description Our Brand L43 Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 µm in diameter ACE C18-PFP, ACME F5/C18, HALO PFP, HPLC/UHPLC Column Selection Guide InertSustain PFP, NUCLEODUR PFP, L44 A multifunctional support, which consists of a high purity, 60 Å, spherical silica substrate that has been bonded with NUCLEOSHELL PFP a cationic exchanger, sulfonic acid functionality in addition to a convention reversed phase C8 funcionality Chromegabond RP-SCX, Legacy L44 L45 Beta cyclodextrin, R,S-hydroxypropyl ether derivative, bonded to porous silica particles, 3 to 10 µm in diameter Nucleodex Beta OH, Nucleodex Beta PM, Shodex ORpak CDBS-453 L46 Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalized latex beads, Please enquire about 9 to 11 µm in diameter L47 High capacity anion-exchange microporous substrate, fully functionalized with a trimethylamine group, Hamilton PRP-X100, Hamilton PRP-X110, Hamilton RCX-10, 8 µm in diameter Hamilton RCX-30 L48 Sulphonated, cross-linked polystyrene with an outer layer of submicron, porous, anion-exchange microbeads, 5 to Please enquire 15 µm in diameter L49 A reversed-phase packing made by coating a thin layer of polybutadiene on to spherical porous zirconia particles, 3 Please enquire to 10 µm in diameter Please enquire Multifunction resin with reverse-phase retention and strong anion-exchange functionalities. The resin consists of L50 ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15 µm in diameter, and a surface area of not less than 350 m2/g. Substrate is coated with quartenary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene L51 Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 3 to 10 µm in diameter CHIRALPAK AD, CHIRALPAK AD-H, CHIRALPAK AD-3 L52 A strong cation exchange resin made of porous silica with sulfopropyl groups, 5 to 10 µm in diameter Please enquire Please enquire Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to Please enquire L53 15 µm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Universal Cation, Universal Cation HR Please enquire Capacity not less than 500 µEq/column L54 A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, 5 to 15 µm in diameter L55 A strong cation exchange resin made of porous silica coated with polybutadiene-maleic acid copolymer, about 5 µm in diameter L56 Propyl silane chemically bonded to totally or superficially porous silica particles, 3 to 10 µm in diameter 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 23 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC USP Chromatographic Columns Listing Description Our Brand L57 A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 µm in diameter, with a pore Please enquire size of 120 Å NUCLEOGEL Sugar Na, Shodex CXpak P-421S, Shodex RSpak L58 Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the DC-613, Shodex SUGAR KS-801, Shodex SUGAR KS-802, Shodex sodium form, about 6 to 30 µm diameter SUGAR KS-803, Shodex SUGAR KS-804, Shodex SUGAR KS-805, Shodex SUGAR KS-806, Shodex SUGAR KS-807 L59 Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 Shodex KW400 series, Shodex PROTEIN KW-800 series kDa. The packing is spherical 1.5 to 10 µm, silica or hybrid packing with a hydrophilic coating L60 Spherical, porous silica gel, 10 µm or less in diameter, the surface of which has been covalently modified with alkyl ACME Amide/C18, HALO RP-Amide, Nucleodur PolarTec, amide groups and endcapped Nucleosil C18 Nautilus A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 13 µm microporous L61 particles having a pore size less than 10 units and consisting of ethylvinylbenzene cross-linked with 55% divinylben- Please enquire zene with a latex coating composed of 85 nm diameter microbeads bonded with alkanol quarternary ammonium ions (6%) L62 C30 silane bonded phase on a fully porous spherical silica, 3 to 15 µm in diameter Chromegabond C30 L63 Glycopeptide teicoplanin linked through multiple covalent bonds to a 100 Å spherical silica Please inquire L64 Strongly basic anion exchange resin consisting of 8% crosslinked styrene divinylbenzene copolymer with a Please enquire quartenary ammonium group in the chloride form, 45 to 180 µm in diameter Please enquire L65 Strongly acidic cation exchange resin consisting of 2% sulfonated crosslinked styrene divinylbenzene copolymer with a sulfonic acid group in the hydrogen form, 63 to 250 µm in diameter L66 A crown ether coated on a 5 µm particle size silica gel substrate. The active site is (S)-18-crown-6-ether CROWNPAKTM CR (+) L67 Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer, 2 to 10 µm in Shodex Asahipak ODP-40, Shodex Asahipak ODP-50, diameter Shodex ET-RP1 L68 Spherical, porous silica, 10 µm or less in diameter, the surface of which has been covalently modified with alkyl InertSustain Amide, Inertsil Amide amide groups and not endcapped L69 Ethylvinylbenzene/divinylbenzene substrate agglomerated with quaternary amine functionalized 130 nm latex Please enquire beads, about 6.5 µm in diameter L70 Cellulose tris(phenyl carbamate) coated on 5 µm silica CHIRALCEL OC-H 24 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide USP Chromatographic Columns Listing Description Our Brand L71 A rigid, spherical polymetacrylate, 4 to 6 µm in diameter Shodex RSpak DE-213, Shodex RSpak DE-413, HPLC/UHPLC Column Selection Guide Shodex RSpak DE-613 L72 (S)-phenylglycine and 3,5-dinitroaniline urea linkage covalently bonded to silica Please enquire L73 A rigid, spherical polydivinylbenzene particle, 5 to 10 µm in diameter Jordi Resolve DVB Please enquire A strong anion-exchange resin consisting of a highly cross-linked core of 7 µm macroporous particles having a Resolvosil BSA L74 100 Å average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion- Please enquire Please enquire exchange layer grafted to the surface, which is functionalized with alkyl quartenary ammonium ions Primesep D, Primesep SB, Primesep B2 L75 A chiral-recognition protein, bovine serum albumin (BSA), chemically bonded to silica particles, about 7 µm in CHIRALPAK AS-H diameter, with a pore size of 300 Å L76 Silica based weak cation-exchange material, 5 µm in diameter. Substrate is surface polymerized polybutadiene- maleic acid to provide carboxylic acid functionalities. Capacity not less than 29 µEq/column Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 6 to L77 9 µm diameter. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 500 µEq/column (4 mm x 25 cm) A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and anion-exchange (primary, L78 secondary, or tertiary amino groups) functional groups chemically bonded to porous or non-porous or ceramic micro-particles, 1 to 50 µm in diameter or a monolithic rod L79 A chiral-recognition protein, human serum albumin (HSA), chemically bonded to silica particles, about 5 µm in diameter L80 Cellulose tris(4-methylbenzoate)-coated, porous, spherical, silica particles, 5 to 20 µm in diameter CHIRALCEL OJ, CHIRALCEL OJ-H, CHIRALCEL OJ-3 A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 9 µm porous particles L81 having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex Please enquire coating composed of 70 nm diameter microbeads (6% crosslinked) bonded with alkanol quartenary ammonium ions L82 Polyamine chemically bonded to cross-linked polyvinyl alcohol polymer, 4 to 5 µm in diameter Shodex Asahipak NH2P-40, Shodex Asahipak NH2P-50 A hydroxide-selective, strong anion-exchange resin-quartenary amine bonded on latex particles attached to a core Please enquire L83 of 10.5 µm microporous particles having a pore size of 10 Å and consisting of ethylvinylbenzene cross-linked with Please enquire 55% divinylbenzene. Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 5 µm L84 diameter. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 8400 µEq/column (5 mm x 25 cm) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 25 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC USP Chromatographic Columns Listing Description Our Brand L85 A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and weak cation-exchange Primesep 100, Primesep 200, Primesep C, Primesep 500 (carboxyl groups) functional groups chemically bonded to porous or non-porous particles, 1 to 50 µm in diameter L86 A 5 µm fused core particle with a highly polar ligand possessing 5 hydroxyl groups tethered to the silica gel Halo Penta-HILIC outer layer L87 Dodecyl silane chemically bonded to porous silica particles, 1.5 to 10 µm in diameter Please enquire L88 Glycopeptide vancomycin linked through multiple covalent bonds to 100 Å spherical silica Please enquire Packing having the capacity to separate compounds with a molecular weight range from 100 – 3000 Da (as deter- Please enquire L89 mined by polyethylene oxide), applied to neutral and anionic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylate ether (surface contains some residual cationic functional groups) L90 Amylose tris-[(S)-alpha-methylbenzylcarbamate] coated on porous, spherical silica particles, 3 to 10 µm in diameter CHIRALPAK AS, CHIRALPAK AS-H, CHIRALPAK AS-3 L91 Strong anion-exchange resin consisting of monodisperse porous polystyrene/divinyl benzene beads coupled with Please enquire quartenary amine. Bead size is 3 to 10 µm Please enquire CHIRACEL OD-RH, CHIRACEL OD-3R A strong anion-exchange resin consisting of a highly cross-linked core of 5 – 9 µm macroporous particles having Please enquire L92 a 100 Å average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion Halo Penta-HILIC InertSustain AQ-C18 exchange layer grafted to the surface, which is functionalized with alkanol quartenary ammonium ions Please enquire Please enquire L93 Cellulose tris(3,5-dimethylphenylcarbamate) reversed phase chiral stationary phase coated on 3 or 5 µm silica gel particles L94 A strong anion-exchange resin consisting of a highly crosslinked 15 µm microporous particles functionalized with very low crosslinked latex (0.5%) to provide alkanol quartenary ammonium ion exchange sites L95 Highly polar alkyl ligand comprising five hydroxyl groups that are chemically bonded to totally porous or superficially porous silica or a monolithic silica rod Alkyl chain, reversed-phase bonded to totally porous or superficially porous silica designed to retain hydrophilic L96 and other polar compounds when using highly aqueous mobile phases, including 100% aqueous, 1.5 µm to 10 µm in diameter Weak cation-exchange resin consisting of a highly cross-linked core of 5.5 µm porous particles having a pore size of L97 2000 Å units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 2400 µEq/column (4 mm x 25 cm) Weak cation-exchange resin consisting of a highly cross-linked core of 8 µm microporous particles having an aver- L98 age pore size of 10 Å units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 46 µEq/column (4 mm x 5 cm) 26 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide USP Chromatographic Columns Listing Description Our Brand L99 Amylose tris-(3,5-dumethylphenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in CHIRALPAK IA, CHIRALPAK IA-3 HPLC/UHPLC Column Selection Guide diameter Please enquire A 55% crosslinked, microporous, hydrophobic resin core (9 µm microporous particles having a pore size of 10 Å) Cosmocore Cholester, Cosmosil Cholester L100 that consists of a bilayer of anion and cation exchange latex. The first layer is fully sulfonated (140 nm) and the (S,S) Whelk-O 1 Please enquire second layer is fully aminated (76 nm) Please enquire Please enquire L101 Cholesteryl groups chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in Please enquire diameter, or a monolithic rod CHIRALCEL OJ-RH L102 (Naproxen, (S,S)Whelk-O 1) 1-(3,5-dinitrobenzamido)-1,2,3,4-tetrahydrophenanthrene covalently bonded to porous CHIRALPAK CBH spherical silica particles, 5 to 10 µm in diameter Please enquire A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 7.5 μm porous Please enquire L103 particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene Please enquire Please enquire electrostatically bonded with hyperbranched alkanol quaternary ammonium ions L104 Triazole groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter L105 A strong anion-exchange resin consisting of a highly cross-linked 9 μm super macroporous (2000 Å) particles functionalized with very low cross-linked latex (0.2%) to provide alkyl quaternary ammonium ion sites Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, L106 5 to 8 μm diameter, macroporous particles having an average pore size of 100 Å. Substrate is surface grafted with carboxylic acid and phosphonic acid functional groups. Capacity not less than 2800 μEq/column (4 mm x 25 cm) L107 Cellulose tris(4-methylbenzoate)-coated porous spherical particles, 3 to 5 μm in diameter, for use with reversed-phase mobile phases L108 A chiral-recognition protein, cellobiohydrolase (CBH), chemically bonded to silica particles, about 5 μm in diameter L109 Spherical particles of porous graphitic carbon, 3 to 30 μm in diameter L110 A strong anion-exchange resin consisting of a highly cross-linked 13 μm microporous (less than 10 Å) particles coated with very low cross-linked latex (0.5%) to provide alkanol quaternary ammonium ion exchange sites L111 Polyamine chemically bonded to porous spherical silica particles, 5 μm in diameter A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 8.5 μm porous L112 particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (5% cross-linked) bonded with alkanol quaternary ammonium ions 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 27 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC USP Chromatographic Columns Listing Description Our Brand L113 A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 7.5 μm porous Please enquire particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene SeQuant ZIC-HILIC with a latex coating composed of 65 nm diameter microbeads (8% cross-linked) bonded with alkanol quaternary Please enquire ammonium ions Please enquire CROWNPAK CR(-) L114 Sulfobetaine graft-polymerized to totally or superficially porous silica, 1.5 to 10 μm in diameter, or a monolithic rod. ACME™ PAH, NUCLEODUR C18 PAH Packing having densely bonded zwitterionic groups with 1:1 charge balance. CHIRALPAK IC, CHIRALPAK IC-3` Please enquire L115 Ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with quaternary amine functionalized Please enquire 275 nm latex microbeads (6% cross-linked), about 8.5 µm in diameter CHIRALCEL OD-R Please enquire L116 Sulfonated ethylvinylbenzene/divinylbenzene substrate agglomerated with hydrophilic quaternary amine COSMOSIL PYE functionalized glycidyl-derivative methacrylate microbeads, approximately 2 to 50 μm in diameter CHIRALPAK IC, CHIRALPAK IC-3 Please enquire L117 A crown ether coated on a 5 μm particle size silica gel substrate. The active site is (R)-18-crown-6-ether L118 Aqueous polymerized C18 groups on silica particles, 1.2 to 5 μm in diameter L119 Cellulose tris-(3,5-dichlorophenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 13 µm microporous L120 particles having a pore size of less than 10 Å units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (8% crosslinked) bonded with alkanol quaternary ammonium ions. Capacity not less than 10 mEq/column (4 mm x 5 cm) L## Ethylhexyl triazone, FluoFix – Fluorocarbon chains chemically bonded to 5 μm spherical silica particles L## Lanatoprost, Chiracel OD-R – Cellulose tris(3,5-dimethylphenylcarbamate) coated on 10 μm silica gel particles Polyethylene Glycol 3350, Aquagel OH 40 – Packing having the capacity to separate compounds with a molecular L## weight range from 10,000 to 200,000 g/mol (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers, composed of a rigid macroporous material with a hydrophilic surface L## Felodipine Extended-release Tablets, COSMOSIL PYE – Pyrenyl groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter, or a monolithic rod L## Atomoxetine Hydrochloride, Chiralpak IC – Cellulose tris-(3,5-dichlorophenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 μm in diameter Sodium Nitrite, IonPac AS12A – A strong anion-exchange resin consisting of a highly cross-linked 9 μm L## supermacroporous (2000 Å) particles functionalized with very low cross-linked latex (0.2%) to provide alkyl quartenary ammonium ion sites 28 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide USP Chromatographic Columns Listing Description Our Brand L## Liquid Glucose, Aminex HPX-42A – Strong cation-exchange resin consisting of sulfonated cross-linked Please enquire HPLC/UHPLC Column Selection Guide styrene-divinylbenzene copolymer in the silver form, average 9 μm in diameter CHIRALCEL OJ-RH L## Rosuvastatin Calcium, Chiracel OJ-RH – Cellulose tris(4-methylbenzoate)-coated porous spherical particles, Please enquire 3 to 5 μm in diameter, for use with reversed phase mobile phases Cosmosil HILIC Please enquire L## Palonosetron Hydrochloride, Chirobiotic-V – Glycopeptide vancomycin linked through multiple covalent bonds Please enquire to 100 Å spherical silica Please enquire Please enquire L## Allantoin, Cosmosil HILIC – Triazol groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter Please enquire EPTACOG alfa, Dionex CarboPac PA100 – Ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) L## agglomerated with quartenary amine functionalized 275 nm latex microbeads (6% cross-linked), about 8.5 μm in diameter EPTACOG alfa, Dionex DNAPac PA100 – Sulfonated ethylvinylbenzene/divinylbenzene substrate approximately L## 12 to 14 μm in diameter agglomerated with hydrophilic quartenary amine functionalized glycidyl-derivative methacrylate microbeads Adenosine, Dionex IonPac CS12A and CG12A - Weak cation-exchange resin consisting of ethylvinylbenzene, L## 55% cross-linked with divinylbenzene copolymer, 5 to 8 μm diameter, macroporous particles having an average pore size of 100 Å. Substrate is surface grafted with carboxylic acid and phosphonic acid functional groups. Capacity not less than 2800 μEq/column (4 mm x 25 cm) Adenosine, Dionex IonPac AG18 - A hydroxide-selective, strong anion-exchange resin consisting of a highly L## crosslinked core of 13 μm microporous particles having a pore size of <10 Å and consisting of ethylvinylbenzene crosslinked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (8% crosslinked) bonded with alkanol quartenary ammonium ions. Capacity not less than 10 μEq/column (4 mm x 5 cm) Adenosine, Dionex IonPac AS18 - A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 7.5 μm macroporous particles having an average pore size of 2000 Å and consisting of L## ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (8% crosslinked) bonded with alkanol quartenary ammonium ions. Capacity not less than 285 μEq/column (4 mm x 25 cm) 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 29 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC Reference Guide for Estimating Loading Capacity based on Column Parameters - Mesoporous Silica Column Column Particle Column Linear Flowrate, Max. Relative Column Solvent Column Solvent Estimated ID (mm) Length Size dp Void Velocity, u Fc (mL/ Injection Sensitivity Efficiency Viscosity Pressure Usage Loading (mm) (µm) Volume to 4.6 mm relative to Capacity 2.1 Volume, Vo (mm/s) min) (cP)* (bar) 4.6 mmID (mg)** 2.1 (mL) (µL) ID of same 2.1 length 0.52 3.0 1.56 3.0 50 5 0.10 1.5 0.19 10 0.21 3000 1.01 18 0.21 2.60 3.0 1.06 4.6 150 5 0.31 1.5 0.19 31 0.21 9000 1.01 55 0.21 3.18 4.6 5.30 4.6 250 5 0.52 1.5 0.19 52 0.21 15000 1.01 91 0.21 2.49 10 7.48 10 50 5 0.21 1.5 0.38 21 0.43 3000 1.01 18 0.43 12.46 10 11.78 20 150 5 0.64 1.5 0.38 64 0.43 9000 1.01 55 0.43 35.34 20 58.90 20 250 5 1.06 1.5 0.38 106 0.43 15000 1.01 91 0.43 47.12 1 141.37 1 50 5 0.50 1.5 0.90 50 1.00 3000 1.01 18 1.00 235.62 2.1 0.12 2.1 150 5 1.50 1.5 0.90 150 1.00 9000 1.01 55 1.00 0.35 2.1 0.52 3.0 250 5 2.49 1.5 0.90 249 1.00 15000 1.01 91 1.00 1.56 3.0 2.60 3.0 50 5 2.36 1.5 4.24 236 4.73 3000 1.01 18 4.73 1.06 4.6 3.18 4.6 150 5 7.07 1.5 4.24 707 4.73 9000 1.01 55 4.73 5.30 4.6 2.49 10 250 5 11.78 1.5 4.24 1178 4.73 15000 1.01 91 4.73 7.48 10 12.46 10 50 5 9.42 1.5 16.96 942 18.90 3000 1.01 18 18.90 11.78 20 35.34 1 150 5 28.27 1.5 16.96 2827 18.90 9000 1.01 55 18.90 58.90 1 47.12 2.1 250 5 47.12 1.5 16.96 4712 18.90 15000 1.01 91 18.90 0.12 2.1 0.35 3.0 50 3 0.02 1.5 0.04 2 0.05 5000 1.01 51 0.05 0.52 3.0 1.56 3.0 150 3 0.07 1.5 0.04 7 0.05 15000 1.01 152 0.05 1.06 4.6 3.18 4.6 50 3 0.10 1.5 0.19 10 0.21 5000 1.01 51 0.21 5.30 2.49 150 3 0.31 1.5 0.19 31 0.21 15000 1.01 152 0.21 7.48 250 3 0.52 1.5 0.19 52 0.21 25000 1.01 253 0.21 50 3 0.21 1.5 0.38 21 0.43 5000 1.01 51 0.43 150 3 0.64 1.5 0.38 64 0.43 15000 1.01 152 0.43 250 3 1.06 1.5 0.38 106 0.43 25000 1.01 253 0.43 50 3 0.50 1.5 0.90 50 1.00 5000 1.01 51 1.00 150 3 1.50 1.5 0.90 150 1.00 15000 1.01 152 1.00 250 3 2.49 1.5 0.90 249 1.00 25000 1.01 253 1.00 50 3 2.36 1.5 4.24 236 4.73 5000 1.01 51 4.73 150 3 7.07 1.5 4.24 707 4.73 15000 1.01 152 4.73 250 3 11.78 1.5 4.24 1178 4.73 25000 1.01 253 4.73 50 3 9.42 1.5 16.96 942 18.90 5000 1.01 51 18.90 50 1.9 0.02 1.5 0.04 2 0.05 7895 1.01 126 0.05 150 1.9 0.07 1.5 0.04 7 0.05 23684 1.01 378 0.05 50 1.9 0.10 1.5 0.19 10 0.21 7895 1.01 126 0.21 150 1.9 0.31 1.5 0.19 31 0.21 23684 1.01 378 0.21 50 1.9 0.21 1.5 0.38 21 0.43 7895 1.01 126 0.43 150 1.9 0.64 1.5 0.38 64 0.43 23684 1.01 378 0.43 250 1.9 1.06 1.5 0.38 106 0.43 39474 1.01 630 0.43 50 1.9 0.50 1.5 0.90 50 1.00 7895 1.01 126 1.00 150 1.9 1.50 1.5 0.90 150 1.00 23684 1.01 378 1.00 * viscosity of eluent is 10:90, acetonitrile-water, v/v at 25 °C ** approx. 5 mg per gram of C18 packing on mesoporous silica and 1.5 mg per gram of C18 packing on pellicular silica. 30 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide Reference Guide for Estimating Loading Capacity based on Column Parameters - Pellicular Silica Column Column Particle Column Linear Flowrate, Max. Relative Column Solvent Column Solvent Estimated HPLC/UHPLC Column Selection Guide ID (mm) Length Size dp Void Velocity, u Fc (mL/ Injection Sensitivity Efficiency Viscosity Pressure Usage Loading (mm) (µm) Volume to 4.6 mm relative to Capacity 1 Volume, Vo (mm/s) min) (cP)* (bar) 4.6 mmID (mg)** 1 (mL) (µL) ID of same 1 length 0.06 2.1 0.18 2.1 50 5 0.024 1.5 0.042 1 0.05 3000 1.01 18.2 0.05 0.29 2.1 0.26 3.0 150 5 0.071 1.5 0.042 4 0.05 9000 1.01 54.5 0.05 0.78 3.0 1.30 3.0 250 5 0.118 1.5 0.042 6 0.05 15000 1.01 90.9 0.05 0.53 4.6 1.59 4.6 50 5 0.104 1.5 0.187 5 0.21 3000 1.01 18.2 0.21 2.65 4.6 1.25 1 150 5 0.312 1.5 0.187 16 0.21 9000 1.01 54.5 0.21 3.74 1 6.23 1 250 5 0.520 1.5 0.187 26 0.21 15000 1.01 90.9 0.21 0.06 2.1 0.18 2.1 50 5 0.212 1.5 0.382 11 0.43 3000 1.01 18.2 0.43 0.29 2.1 0.26 3.0 150 5 0.636 1.5 0.382 32 0.43 9000 1.01 54.5 0.43 0.78 3.0 1.30 3.0 250 5 1.060 1.5 0.382 53 0.43 15000 1.01 90.9 0.43 0.53 4.6 1.59 4.6 50 5 0.499 1.5 0.897 25 1.00 3000 1.01 18.2 1.00 2.65 4.6 1.25 1 150 5 1.496 1.5 0.897 75 1.00 9000 1.01 54.5 1.00 3.74 1 6.23 1 250 5 2.493 1.5 0.897 125 1.00 15000 1.01 90.9 1.00 0.06 2.1 0.18 2.1 50 2.7 0.024 1.5 0.042 1 0.05 5556 1.01 62.3 0.05 0.29 2.1 0.26 3.0 150 2.7 0.071 1.5 0.042 4 0.05 16667 1.01 187.0 0.05 0.78 3.0 1.30 3.0 250 2.7 0.118 1.5 0.042 6 0.05 27778 1.01 311.7 0.05 0.53 4.6 1.59 4.6 50 2.7 0.104 1.5 0.187 5 0.21 5556 1.01 62.3 0.21 2.65 4.6 1.25 150 2.7 0.312 1.5 0.187 16 0.21 16667 1.01 187.0 0.21 3.74 6.23 250 2.7 0.520 1.5 0.187 26 0.21 27778 1.01 311.7 0.21 50 2.7 0.212 1.5 0.382 11 0.43 5556 1.01 62.3 0.43 150 2.7 0.636 1.5 0.382 32 0.43 16667 1.01 187.0 0.43 250 2.7 1.060 1.5 0.382 53 0.43 27778 1.01 311.7 0.43 50 2.7 0.499 1.5 0.897 25 1.00 5556 1.01 62.3 1.00 150 2.7 1.496 1.5 0.897 75 1.00 16667 1.01 187.0 1.00 250 2.7 2.493 1.5 0.897 125 1.00 27778 1.01 311.7 1.00 50 2 0.024 1.5 0.042 1 0.05 7500 1.01 113.6 0.05 150 2 0.071 1.5 0.042 4 0.05 22500 1.01 340.9 0.05 250 2 0.118 1.5 0.042 6 0.05 37500 1.01 568.1 0.05 50 2 0.104 1.5 0.187 5 0.21 7500 1.01 113.6 0.21 150 2 0.312 1.5 0.187 16 0.21 22500 1.01 340.9 0.21 250 2 0.520 1.5 0.187 26 0.21 37500 1.01 568.1 0.21 50 2 0.212 1.5 0.382 11 0.43 7500 1.01 113.6 0.43 150 2 0.636 1.5 0.382 32 0.43 22500 1.01 340.9 0.43 250 2 1.060 1.5 0.382 53 0.43 37500 1.01 568.1 0.43 50 2 0.499 1.5 0.897 25 1.00 7500 1.01 113.6 1.00 150 2 1.496 1.5 0.897 75 1.00 22500 1.01 340.9 1.00 250 2 2.493 1.5 0.897 125 1.00 37500 1.01 568.1 1.00 * viscosity of eluent is 10:90, acetonitrile-water, v/v at 25 °C ** approx. 5 mg per gram of C18 packing on mesoporous silica and 1.5 mg per gram of C18 packing on pellicular silica. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 31 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC Preparative Columns • Available in 5 μm, 10 μm and 15 μm particles • High Loading Capacity • Up to 100 mm ID available • Optimized Packing Efficiency • Wide variety of stationary phases • Narrow Peak Profile, High Efficiency & Resolution Successful scale-up in Preparative HPLC requires that the Preparative HPLC method be consistent with the Analytical HPLC method, in particular, the phase material, column dimensions and method conditions. Canadian Life Science provides a variety of HPLC stationary phases for your preparative HPLC when scaling up for isolation and purification methods. Prep columns are designed for high sample loading, high throughput applications using silica-based packing materials for pharmaceutical and biopharmaceutical chromatographic purifications. • High mass loading and excellent resolution • Excellent durability with acidic and alkaline resistance • Analytical columns available for screening and method development of isolation and purification methods prior to scale-up Prep Columns – Dimensions and Chemistries Columns are available in various dimensions: • Length: 50 mm, 100 mm, 150 mm and 250 mm length • Diameter: 10 mm, 20 mm, 21.2 mm, 30 mm, 50 mm and larger ID upon request Preparative Packing Characteristics Packing Material Particle Size (µm) Pore Size (Å) End Capped ACME 5, 10 120, 200 Yes ACE 5, 10, 15 100, 300 Yes InertSustain Yes Inertsil 3, 5 100 Yes 3, 5, 10 100 Choosing Preparative Columns The large-scale separation will be based on the analytical column and method conditions used for the small-scale (lab) separation. Ideally, they should be identical. Step 1: Optimize analytical separation Step 2: Determine loading capacity of the analytical column and the chosen packing material. Step 3: Determine how much analyte mass you need to purify or isolate Step 4: Determine the required Prep column size for isolation or purification. See equations below. If preparative columns are packed with the identical media to their analytical counterpart then the theoretical calculations for scale- up will be accurate. The scale-up factor is related to the column length and diameter: Scale-Up Factor Calculation Flow Rate Calculation (ID, Preparative)2 x (Length, Preparative) Flow Rate (Prep.) = Flow rate (Anal.) x (ID, Preparative)2 Scale-Up Factor = (ID, Analytical)2 x (Length, Analytical) (ID Analytical )2 Scale-Up Example: Flow Rate Example: For a 150 mm x 4.6 mm ID Analytical Column For a 150 mm x 4.6 mm ID Analytical Column to a 150 mm x 10 mm ID Preparative Column to a 150 mm x 10 mm ID Preparative Column Scale-Up Factor = (10 mm)2 x (150 mm) = 4.73 or approximate 5-fold scale-up Flow Rate (Prep.) = 1.0 mL/min. x (10 mm)2 = 4.72 mL/min (4.6 mm)2 x (150 mm) (4.6 mm)2 Contact us for more information on availability of prep options, packings, or to discuss your application and the ability to scale up. Our technical experts will be happy to discuss your needs with you. 32 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide e Cost-Benefit Analysis mponent ofMBloenbdiPlerepPahraatisone PrePrpepaarraedtion PL&aubrcURhesaasedeyd contamination and exposure that is common with in-house Blend blending by hand. It also helps to eliminate variation, erials: Prepare fresh and store $in2a9,r0e0se0rvoir w$h3ic3h,3is50inert and freewohf iccohntamalilnoawtiosn. Iyf omuobrilebplehansde sis ptroemibxeed (oarsganpicr+ecaqisueeouas sbuffer) ers / year) filter before placing it in reservoir. Store at ambient temperaptourses–ibelleeva–tebdarotcomh taefmteprerbatautrceshr,eaduncde mlootbialeftpehrasloe ts.helf life. prepare blend: $10,375 $ 0 HPLC/UHPLC Column Selection Guide mserer/uiblyaaeltlesiaonrnfdaoetrrd$rro2iwTdHser5orsocfhaopan(iimaemcrresrmhdsaypheuutwaormidluolcaeleraovb)tlslliwello’e5synsc%nBro:dyeulnoosdrss:udusirsicrctaebketslene&)tcnhyJde,a$$secctkno1oshn,o45batn15aen™90mcaesi(nBspa&artfeiJeo)ctniL$$ysaeaabnanRdsdepeasxod00apsyvsoeBisbltueliermne–detsbh®waaaettcriAHBrhesesclfPaceooofLtrmnrmemeCdrmmuqbsloeuaannttriacerdewhderqt,iedtuoahusnuitibbndsrelgeeeylsonamLttdcaepaibeunrdfRotngseepttbrtarosyoildeo,myhtta®.amreAnaBydsr,esle.cresnelIottqpdswauseeliissdercoe-ollidlohfcioebcrlpyapaasbscttultiiecoosntmneodalmsiinmon,egsdirLnttesaapctgehbeLrcnvRaiCoafidelrco-iaiaagMettyidino,oSywnnts,h, ich at $25 per hLoaubr)Ready Blends are tested to meet isocratic and gradient HrePLqCurierqeumireemnetnst.s, as well as most LC-MS requirements. seupraoflosrbahllea:nzadrdioFnoorgurcscomwonoastsrtatseec:)tininfoform@alitfieosnc,ipe$$nlec4ae1s.e,1c4a0v4.0i4sit the H$oneyw0ell Research Chemicals section of our website www.lifescience.ca $ 33,350 aving with LabReady Blends: 20% FlexFibleilxiitbyilaitnydanPdrePcreisciiosinonoof fLLaabbRReeaaddyyBBlelenndds s productivity gained: 24% Mobile phase for testing warfarin: 27.2 grams of potassium phosphate Type of Component Available in Formulation odium hydroxide in water with a pH of 7.4 using phosphoric acid LabReady Blends Tolerance Solvent, aqueous and organic rison of Analysis Quality Salt, organic and inorganic ± 1.0% Acid and base Manual Blend pH buffer ± 10% * ± 10% * ± 0.1 9-peptide resolution * Based on concentrations less than 1% of the final blend with 0.06% formic acid Note: All components are available in varying grades of purity, from reagent or ACS general use to higher LC-MS grades, with varying grades in-between. Component grades are determined based on the application and/or customer specifications. LabReady Blends are available with a variety Enhance Safety of packaging options - from bottles to bulk sizes. Manual Blending StepsWEhliimleiLnaabteRdeawdiythBlLeanbdRseealidmyinBalteentdhes costs and time associated with manual blending, they also remove mple human error, one lab attempting to produce 0.1% formic the safety hazards associated with manual blending uced a lower-than-intended 0.06% formic acid concentration, in your laboratory. With fewer materials and less n atypical separation. LabReady Blends preparation required, LabReady Blends help you reduce many of the risks associated with the storing, 9-peptide resolution Order and AssemblheandlinMg,eabsulerenadnidng anWdeigdhissoploidssing of hazardous with 0.1% formic acid inventory glassware asnodlvents,maixcliidqusidasnd otherancdhmemix icals. materials equipment A Commitment to Technical Service As experts in laboratory chemical technology, Burdick & Jackson’s technical support scientists are always ready to assist you with the challenges in your lab – over the phone or even on-site in your Test and Filter and delagabsoratorCy.leaCnounptact usPetrofodramyfinsaol we can begin to accurate 0.1% formic acid concentration, rtehseuHlatsod.njeuyswtepll HLabReady® solutionassist ymoautewriaitlhs:and quality check duced the expected, accurate separation (optional)• Formeuqluaitpimonenst pecificoartuiosnesteastnd customization • Applications ve Consiste1n8c8y8-2a2n6-d277Q5 u:a: [email protected] :: www••.lifesBScieoonltvctelee.cnaat nsdelebcutlkiopnackaging optiLCoInFAsENSACDIEINACNE 33

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC Common Mobile Phase Solvent Properties HPLC mobile phase composition usually consists of a blend of two or more solvents – most commonly it is a blend of one strong solvent and one weak solvent. Strong Solvent - by convention called mobile phase B Weak Solvent - by convention called mobile phase A • Increasing the strong solvent decreases analyte retention • The weak solvent increases analyte retention • Typical in reversed-phase (RP) mode: acetonitrile, methanol • Typical in reversed-phase (RP) mode: water, aqueous buffer The Mobile Phase serves as the eluent or solvent carrier. The mobile phase interacts with the analyte and the stationary phase (the column) to produce the separation. Mobile Phase affects separation efficiency, retention, selectivity and detection. Properties of Selected Mobile Phase Solvents Solvent UV Cutoff (nm) Refractive Index (20 °C) Viscosity (cP) Boiling Point (°C) Dielectric Constant Water* 185 1.3330 1.00 100 80 Acetonitrile* 190 1.3441 0.38 81.6 38 Methanol* 205 1.3284 0.55 64.7 33 Ethanol* 210 1.3615 1.20 78.3 25 Tetrahydrofuran* 212 1.4072 0.55 66.0 7.6 Acetone* 330 1.3587 0.36 56.3 21 Ethyl acetate 256 1.3724 0.45 77.1 6.1 Dichloromethane 233 1.4241 0.44 39.75 7.8 Isopropanol* 205 1.3772 2.4 82.26 18.6 Hexane 195 1.3749 0.31 68.70 1.9 Selecting the proper grade and purity for the application is critical. Example of specification comparison for Acetonitrile Product Code Chromasolv™ LC-MS Ultra, Chromasolv™ LC-MS Chromasolv™ Plus, Chromasolv™, for HPLC, Chromasolv™, for LC tested for UHPLC-MS for HPLC gradient grade HW-34881 GC Purity HW-14261 HW-34967 HW-34998 HW-34851 min. 99.8% min. 99.9% min. 99.9% min. 99.9% min. 99.9% GC-ECD (heptachlor expoxide) max. 0.0002% max. 1 ppm max. 0.0002% max. 10 ng/L max. 0.0005% max. 0.01% Non-volatile matter max. 0.01% max. 0.01% max. 0.0002% max. 0.02% max. 0.001% max. 0.001% max. 0.001% max. 0.01% max. 0.001% Water content (Karl Fischer) max. 0.0001% max. 0.0001% max. 0.001% max. 0.0002% N/A max. 0.3 ppb max. 0.5 ppb max. 0.0001% max. 1 ppb Free acid (as CH3COOH) 254 nm max. 0.3 ppb max. 0.5 ppb max. 0.5 ppb max. 0.5 ppb Transmittance Free alkali (as NH3) 265 nm Transmittance Absorbance max. 0.5 ppb Absorbance min. 30% Absorbance min. 50% Fluorescence (chinin) min. 85% max. 0.097 max. 0.12 min. 90% max. 0.022 max. 0.10 max. 0.032 191 nm min 97% max. 0.02 min. 99% 195 nm min. 98% 200 nm UV 215 nm min. 99% max. 004% max. 0.005 max. 0.0044 220 nm max. 0.0044 max. 0.0044 Gradient 228 nm Baseline drift 230 nm UHPLC HPLC max. 0.0044 max. 0.0044 235 nm max. 2 mAU peak max. 1 mAU max. 0.0044 max. 0.0044 240 nm max. 8 mAU drift 250 nm HPLC HPLC HPLC 400 nm UHPLC max. 0.2 mAU max. 1 mAU max. 3 mAU max. 0.6 mAU peak 210 nm max. 12 mAU HPLC HPLC max. 3 mAU drift max. 0.2 mAU max. 0.5 mAU 254 nm max. 12 mAU max. 15 mAU 210 nm 34 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide Buffer Retention, k' Figure 1. pH Effect on Retention é k' Neutral HPLC/UHPLC Column Selection Guide k' Acid In reversed-phase LC, the pH of the eluent can significantly 40 k' Base influence the separation of components. Buffers are required 35 when the sample contains ionic or ionizable analytes. Without 30 Robust pH a buffer, poor peak shape and variable retention may result. 25 In general, for acids, increasing eluent pH leads to increased 20 ionization and a decrease in retention (see Figure 1). For 15 bases, decreasing pH results in greater ionization and 10 ê decreased retention. For robust methods, separations should be developed at a pH where retention is least affected by pH 5 change (see robust pH zones in Figure 1). Choice of buffer pH Optimum buffering capacity occurs at a pH equal to the pKa 0 0.0 2.0 4.0 6.0 8.0 10.0 12.0 of the buffer. In general, the effective pH range for a buffer is within ± 1 pH unit of itʼs pKa. Table 1 indicates the pH range of pH some common buffers. The buffer pH should be selected so that it is at least ± 1 pH units from the pKa of the analyte. This will Buffers for LC-MS ensure that the analytes are 100% ionized or 100% non-ionized, for reproducible retention. For LC-MS analyses, volatile buffers or additives are preferred, in order to minimize MS ion suppression and maintain sensitivity. Further considerations In addition, non-volatile buffers such as phosphate may lead to contamination of the ion source. Buffer concentrations should At pH >7, phosphate buffers accelerate the dissolution of silica. be as low as possible eg 10 to 20mM. Ammonium salts are more For optimum column lifetime, organic buffers (e.g. pyrrolidine) volatile than those of Na+ or K+. TFA should be avoided with should be used for pH above 8. Buffer concentrations in the 10 - electrospray LC-MS as it reduces sensitivity. Formic acid or acetic 25mM range are recommended (maximum 50mM). Fresh buffer acid (0.01 to 1% v/v) are preferred. For higher pH applications, solutions should be prepared regularly and pre-column filters ammonium hydroxide is recommended. should be used to protect columns. Buffers should be flushed from the column prior to storage. Table 1 Buffer or Additive pKa (25 °C) pH range UV Cut-off (nm) LC-MS Compatible Trifluroacetic acid 0.3 1.5–2.5 210 (0.1%, v/v) Yes Phosphoric acid (pKa1) 2.1 1.1–3.1 <200 (10 mM) No Phosphoric acid (pKa2) 7.2 6.2–8.2 <200 (10 mM) No Phosphoric acid (pKa3) 12.3 11.3–13.3 <200 (10 mM) No Citric acid (pKa1) 3.1 2.1–4.1 230 (10 mM) No Citric acid (pKa2) 4.8 3.8–5.8 230 (10 mM) No Citric acid (pKa3) 6.4 4.4–6.4 230 (10 mM) No Formic acid 3.8 2.8–4.8 210 (10 mM) Yes Acetic acid 4.8 3.8–5.8 210 (10 mM) Yes Trisaminomethane (TRIS) 8.1 7.1–9.1 205 (10 mM) No Borate 9.2 8.3–10.3 <200 (10 mM) No Dimethylamine 10.7 9.7–11.7 <200 (10 mM) Yes Triethylamine 10.8 9.8–11.8 <200 (10 mM) Yes Ammonium hydroxide 9.2 8.2–10.2 <200 (10 mM) Yes Pyrrolidine 11.3 10.3–12.3 <205 (10 mM) Yes N-Methyl pyrrolidine 10.3 9.3–11.3 <205 (10 mM) Yes Piperidine 11.1 10.1–11.1 <205 (10 mM) Yes Ammonium acetate 3.8–5.5 210 (10 mM) Yes Ammonium bicarbonate (pKa1) 6.4 5.4–7.4 <200 (10 mM) Yes Ammonium bicarbonate (pKa2) 10.3 9.3–11.3 <200 (10 mM) Yes Ammonium 9.2 8.2–10.2 <200 (10 mM) Yes 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 35 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC HPLC Column Chemical Protection • Compatible with any HPLC/UHPLC column • Female inlet/male outlet design provides ultra-low dispersion for HPLC/UHPLC columns • Physically filters particulate material that leads to column inlet fritGpualrud gCogluinmg ACME Guard Application 1 • Retains non-eluting compounds in a sample and reduces column fouling Column: ACME C18, 3 µm, 150 x 4.6 mm ID • Available in 5 mm and 10 mm lengths Guard: ACME Guard, 10 x 4.6 mm ID • Available in a variety of phase chemistries Mobile Phase: Acetonitrile:Water, (70:30, v/v) Sample: • Pre-injection filter: Flow rate: 1.5 mL / min Peak Name RT RT * When used as pre-injection filter (5 mm x 4.6 mm ID, 5 µm),Temperature: 25 °C 1 Uracil (No Guard) (with Guard) Detection: UV @ 265 nm 2 Benzophenone 1.04 1.12 2.91 3.15 the guard can filter out mobile phase impurities and debris ItnhjecatitoncVaolnumpe:r3eµmL aturely shorten colu3mn Nliafpeht.halene 3.59 3.88 It can be placed in-line prior to the injector with no significant impact on column back pressur4e. Biphenyl 4.58 4.96 Figure 1 With Guard 2 Peak 4 - With Guard 3 k’ = 3.41 Column: C18, 3 µm, 150 x 4.6 mm ID 1 N = 23,154 Guard: 10 x 4.6 mm ID As = 1.01 Mobile Phase: Acetonitrile:Water, (70:30, v/v) Without Guard P = 3495 psi (241 bar) Flow rate: 1.5 mL / min Temperature: 25 °C 1 4 Detection: UV @ 265 nm Injection Volume: 3 µL 2 3 ACME Guard Application 2 Column: APCeMakE C18, 1.9 µm, 1N00amx e2.1 mm ID RTSample: BenzodiaRzeTpines Peak 4 - No Guard (No Gu1a. rdO) xazepam(with Guard) k’ = 3.39 Guard: ACME Guard, 5 x 2.1 mm ID 1.024. Alprazolam 1.12 4 N = 21,340 Mobile Phas1e: Acetonitrile:WaUterra,c(4il0:60, v/v) 2.9431.. FClluonniatrzaezpeapmam3.15 Flow rate: 0.24 mL / min Benzophenone 3.559. Diazepam 3.88 As = 1.00 P = 3278 psi (226 bar) 4.58 4.96 Temperature3: 25 °C Naphthalene Detection: U4V @ 254 nm Biphenyl Injection Volume: 1.5 µL With Guard Figure 1: Comparison of C18, 3 µm, 150 x 4.6 mm ID with and without guard column. Addition of the guard column shows no loss of efficiency compared to the C18 column without the guard column installed. Retention (k’) is changed less 1% with the guard and Selectivity (α) is not affected (see Peak 4). Without Guard Figure 2 Figure 2: Comparison of C18, 1.9 µm, 100 x 2.1 mm ID with and without guard column. Guard column shows no loss of efficiency. Selectivity is also unaffected compared to the C18 column without the guard column installed. Column: C18, 1.9 µm, 100 x 2.1 mm ID Guard: 5 x 2.1 mm ID Mobile Phase: Acetonitrile:Water, (40:60, v/v) Flow rate: 0.4 mL / min Temperature: 25 °C Detection: UV @ 254 nm Injection Volume: 1.5 µL Sample: Benzodiazepines 1. Oxazepam 2. Alprazolam 3. Clonazepam 4. Flunitrazepam 5. Diazepam 36 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide HPLC Column Physical Protection - Pre-column filters UltraShield HPLC/UHPLC Precolumn Filters In-Line HPLC/UHPLC Precolumn Filters • Economical protection against micro-particles • Cost effective way to extend column lifetime HPLC/UHPLC Column Selection Guide • Extends column lifetime • Replaceable filter element • Negligible effect on column performance • Negligible effect on column performance • Universal fit, connects directly to column • Universal fit, connects directly to column • Leak tight to 15,000 PSI • Leak tight to 15,000 PSI • Available with 0.5 and 2 micron filters • Available with 0.2, 0.5, 2 and 5 micron filters • Available with SS or titanium filters (0.2 µm) (2 µm) (0.5 µm) (2 µm) AS-850-1027 AS-850-1026 AS-850-1010 AS-850-1015 AS-850-1027T AS-850-1026T AS-850-1010T AS-850-1015T (0.5 µm) (5 µm) AS-850-1025 AS-850-1028 AS-850-1025T AS-850-1028T UltraShield direct connect HPLC/UHPLC Precolumn Filters The In-Line precolumn filter was engineered specifically for provide economical protection from the harmful effects of use in high throughput and routine HPLC separations where sample and mobile phase particulates on modern UHPLC and mobile phase and sample contamination cause a decrease in other high resolution HPLC columns. UltraShield was engineered column performance or premature column failure. The low specifically for use in fast, high efficiency separations requiring swept volume of the InLine filter maintains the efficiency of high mobile phase velocities and ultra-high pressure. UltraShield high performance columns assuring a negligible loss of critical maintains the efficiency of these high performance columns resolution. With its simple design, the filter installs in any assuring no loss of critical resolution. With its simple design, analytical column in seconds and is leak tight to 15,000 PSI. UltraShield installs in any analytical column in seconds and is Simply finger tighten initially, then wrench tighten an additional leak tight to 15,000 PSI. Simply tighten the filter with a wrench 1/4 - 1/2 turn. until it is snug, followed by an additional 1/4 turn. ColumnShield HPLC FingerTight Precolumn Filters In-Line & Direct Connect HPLC/UHPLC Precolumn Filters • Economical protection against micro-particles • Cost effective way to extend column lifetime • Extends HPLC column lifetime • Replaceable filter element • Negligible effect on column performance • Negligible effect on column performance • Universal fit, connects directly to column • Universal fit, connects directly to column • Fingertight to 5,000 PSI • Leak tight to 15,000 PSI • Available with 0.5 and 2 micron titanium filters • Available 0.2, 0.5, 2 and 5 micron filters • Available with SS and titanium filters (0.5 µm) (2 µm) (0.2 µm) (2 µm) AS-850-1050T AS-850-1051T AS-850-1022 AS-850-1021 AS-850-1022T AS-850-1021T (0.5 µm) (5 µm) AS-850-1020 AS-850-1023 AS-850-1020T AS-850-1023T ColumnShield HPLC FingerTight Precolumn Filter provides Recommended for applications where system volume must be economical protection from the harmful effects of sample kept to a minimum. Full use of the filter surface is maintained and mobile phase particulates on modern HPLC columns. by a 5° entrance cone. The 0.25” diameter replacement filter The disposable filter was engineered to maintain the efficiency cartridges are available in 0.2 micron, 0.5 micron, 2 micron, 10 of high performance columns assuring no loss of critical micron and 20 micron porosity, and have a 0.32 cm² active area. resolution. With its simple design, the filter installs in any Total internal volume is 11 µL for the 0.2 micron filter, 14 µL for analytical column or guard column in seconds and is leak tight the 0.5 micron filter, 18 µL for the 2 micron filter and 27 µL for to 5,000 PSI. To install simply hand tighten the filter as you would the 20 micron filter. with any finger tight fitting while holding the column stable. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 37 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLCGuide pour la Sélection de Colonnes HPLC/UHPLC Column Cleaning and Storage by Chromatographic Mode Column Cleaning and Storage Residual ion-pairing reagents, acids, or buffer salts in the column encourage hydrolysis of the bonded phase resulting in shorter column lifetimes. Additionally, storing columns in highly aqueous mobile phases for extended periods of time can lead to peak splitting and reduced column efficiency as the sample matrices such as lipids can accumulate in the column. To prolong column lifetimes, clean the column every single time after the analysis or whenever possible. Cleaning Reversed-Phase Columns When the mobile phase does not contain any buffer or ion-pairing reagents, use a high concentration of organic solvent to remove highly lipophilic contaminants, e.g., 100% organic solvent. Then, flush the column with 5 column volumes. When observing excessive back pressure, reduce and adjust the flow rate. Example Column Dimension – 4.6 mm ID x 250 mm Method Flow Rate – 1 mL/min Method Mobile Phase – Acetonitrile/water = 65/35 Step 1: Clean the column with 100% acetonitrile at 1 mL/min for at least 30 minutes and store at room temperature. When the mobile phase contains a buffered mobile phase, clean the column with a water/organic solvent mixture using the same content as in the buffered mobile phase. For example, if the mobile phase is 80/20, buffer/acetonitrile, clean the column with 20% acetonitrile in water for at least 30 minutes. Then clean it with 100% acetonitrile. Failure to perform this intermediate step could result in precipitation of the buffer salt in the column when 100% acetonitrile is introduced. Example Column Dimension – 4.6 mm ID x 250 mm Method Flow Rate – 1 mL/min Method Mobile Phase – 10 mM KH2PO4/acetonitrile = 80/20 Step 1 Clean the column with 20% acetonitrile in water at 1 mL/min for at least 30 minutes Step 2 Clean the column with 100% acetonitrile at 1 mL/min for at least 30 minutes and store at room temperature When you are ready to use the column again: Step 3 Equilibrate the column with the buffered mobile phase to be used at 1 mL/min for at least 30 minutes Step 4 The column may be considered fully equilibrated once a constant back pressure and stable baseline are observed Mobile phases containing ion-pairing reagents Depending on the ion-pairing reagent type, precipitation may occur when cleaning the column with 100% water and extreme care is required. Clean the column with a water/organic solvent mixture, using the same content as in the mobile phase containing an ion-pairing reagent. For example, clean the column with 10% acetonitrile in water for at least 30 minutes. Then, clean it with 50/50, water/acetonitrile for at least 30 minutes. The content of organic solvent should be increased further when using ion-pairing reagents containing long alkyl chains to effectively remove out from the column. Example Column Dimension – 4.6 mm ID x 250 mm Method Flow Rate – 1 mL/min Method Mobile Phase – 10mM KH2PO4 + 2 mM *IPCC-09 (pH: 2.5)/acetonitrile = 90/10 Step 1 Clean the column with water/acetonitrile = 90/10 at 1 mL/min for at least 30 minutes Step 2 Clean the column with water/acetonitrile = 50/50 at 1 mL/min for at least 30 minutes * IPCC-09: Sodium 1-nonanesulfonate * Please be aware that removal of 100% of the ion-pairing reagent may not be possible. Due to the fact that ion-pairing reagents can alter column selectivity, it is strongly recommended to dedicate columns to ion-pairing methods to avoid problems with reproducibility. 38 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Column Selection Guide Cleaning and Storage by Chromatographic Mode HPLC/UHPLC Column Selection Guide Cleaning HILIC Columns Under HILIC mode, polar analytes are retained using a high percentage of organic modifier in the mobile phase. The following describes the elution strength of solvents used in HILIC mode. Tetrahydrofuran < Acetonitrile < 2-Propanal < Ethanol < Methanol < Water Weak solvents Strong solvents To avoid precipitating mobile phase buffers within the column, clean the column with a water/organic solvent mixture using the same content as in the buffered mobile phase. Clean the column with 50/50, acetonitrile/water to remove highly polar contaminants. If the column still shows shift in retention time or distorted peak shapes, clean the column with 100% water for at least 30 minutes. After cleaning the column, make sure to thoroughly equilibrate the column with the mobile phase to be used in the analysis prior to use. Store the column in 100% acetonitrile. Example Column Dimension – 4.6 mm ID x 250 mm Method Flow Rate – 1 mL/min Method Mobile Phase – 5mM CH3COONH4/acetonitrile = 10/90 Step 1 Clean the column with acetonitrile/water = 90/10 at 1 mL/min for at least 30 minutes Step 2 Clean the column with acetonitrile/water = 50/50 at 1 mL/min for at least 30 minutes Cleaning Normal-Phase Columns Normal-phase columns will retain polar analytes more strongly than non-polar analytes. Clean the column with polar solvents to remove highly polar contaminants. The following describes the elution strength of solvents used in normal-phase mode. Hexane < Chloroform < Tetrahydrofuran < 2-Propanol < Ethanol Weak solvents Strong solvents Clean the column with ethanol or 2-propanol. Because alcohol solvents are quite viscous, adjust the flow rate to avoid excessive column back pressure. Example Column Dimension – 4.6 mm ID x 250 mm Method Flow Rate – 1 mL/min Method Mobile Phase - n–hexane/2-propanaol/acetic acid = 90/10/0.1 Step 1 Clean the column with 100% 2-propanol at 0.2 mL/min for at least 60 minutes Column Storage Residual ion-pairing reagents, acids or salts in the column will promote and encourage hydrolysis of the bonded phase resulting in shorter column lifetimes. Additionally, storage in highly aqueous mobile phases for a long period can lead to splitting peaks or less column efficiency as the sample matrices such as lipids can accumulate in the column. To prolong column lifetimes, clean the column every single time after the analysis or whenever possible. After cleaning the column properly, store the column by referring to the following table. Bonded Phase Storage from 1-10 days Storage longer than a few weeks C18, C8, C4, Ph, HILIC, Amide Mobile Phase (without salts, additives) 100% Acetonitrile Mobile Phase (without salts, additives) 100% n-Hexane CN, NH2, Diol, Silica Mobile Phase (without salts, additives) 100% Acetonitrile Ion-Exchange * Columns should be stored in a cool and dark place. * Columns stored for a long period of time should be cleaned prior to use. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN 39 LIFE SCIENCE

Guide pour la Sélection de Colonnes HPLC/UHPLC Guide pour la Sélection de Colonnes HPLC/UHPLC Solvent Properties Viscosity Boiling UV Polarity Eluotropic Values (e°) Refractive Flash Density (cP) Point Cutoff Index Index Point (g/mL) Solvent 20°C Solvent Alumina C18 Silica 20°C 20°C °C nm P’ Group °F Acetone 0.7900 Acetonitrile 0.36 56.29 330 5.1 6a 0.56 8.8 0.53 1.3586 -4 0.7822 Benzene 0.38* 81.60 190 0.8765 n-Butyl Acetate 0.652 80.1 255 5.8 6b 0.65 3.1 0.52 1.3441 42 0.8796 n-Butyl Alcohol 0.74 126.11 254 0.8097 n-Butyl Chloride 2.98 117.5 215 — 7 — — — — 11 0.8862 Carbon Tetrachloride 0.45 78.44 220 1.5867 Chlorobenzene 76.72 4.0 — — — — 1.3942 72 1.1058 Chloroform — 131.69 — 1.4892 Cyclohexane 0.80 61.15 287 3.9 2 0.7 — — 1.3993 98 0.7785 Cyclopentane 0.57 80.72 245 0.7454 o-Dichlorobenzene 1.0 49.26 200 1.0 5 0.26 — 0.20 1.4021 15 1.3058 1,2 Dichloroethane 0.44 180.48 198 1.253 Dichloromethane 1.32† 295 — – — — — 1.4601 — 1.326 Dimethyl Acetamide 0.84 84 0.9415 N,N-Dimethylformamide 0.44 39.75 — 2.7 7 0.30 — — 1.5248 82 0.9487 Dimethyl Sulfoxide 2.14 166.1 233 1.1004 1,4-Dioxane 0.92 153.0 268 4.1 8 0.40 — 0.26 1.4458 — 1.0336 Ethyl Acetate 2.24 189.0 268 0.9006 Ethyl Alcohol 1.37 101.32 268 0.2 0 0.04 — 0.03 1.4262 -4 0.7892 Diethyl Ether 0.45 77.11 215 0.7133 Ethylene Dichloride 1.10† 78.32 256 0.1 0 0.05 — — 1.4064 19 1.253 Heptane 0.24 34.55 210 0.6837 Hexane 0.79 83.48 215 2.7 — — — — 1.5514 151 0.6594 Iso-Octane 0.42 98.43 228 0.6919 Isobutyl Alcohol 0.31 68.7 200 —5————— 0.8016 Isopropyl Alcohol 0.50 99.24 195 0.7854 Isopropyl Myristate 3.95 107.7 215 3.1 5 0.42 — 0.30 1.4241 — 0.8532 Methanol 2.4 82.26 220 0.7913 2-Methoxyethanol 192.6 205 6.5 3 — — — 1.4384 158 0.965 Methyl t-Butyl Ether — 64.7 0.7405 Methyl Ethyl Ketone 0.59 124 — 6.4 3 — 7.6 — 1.4305 136 0.8049 Methyl Isobutyl Ketone 1.72 55.2 205 0.8008 N-Methylpyrrolidone 0.27 79.64 7.2 3 0.62 — — 1.4793 190 1.0304 n-Octanol 0.43 117.4 — 0.824 Pentane 0.58 202.0 210 4.8 6a 0.56 11.7 0.51 1.4224 54 0.6262 Petroleum Ether 1.67† 195 329 n-Propyl Alcohol 36.07 334 4.4 6a 0.58 — 0.48 1.3724 24 0.64 Propylene Carbonate — 285 0.8037 Pyridine 0.23 — — — 2 — — — 1.3614 59 1.2006 Tetrachloroethylene 97.2 190 0.9832 Tetrahydrofuran — 241.7 — 2.8 1 0.38 — 0.43 1.3524 -49 1.622 Toluene 2.3 115.25 210 0.888 1,2,4-Trichlorobenzene — 121 220 3.5 5 0.49 — — 1.4448 56 0.8669 Triethylamine 0.95 66.0 — 1.454 Trifluoroacetic Acid 0.89 110.62 — 0.1 0 — — 0.01 1.3876 25 0.7276 2,2,4 Trimethylpentane 0.55 213.5 212 1.4890 Water 0.59 89.5 285 0.1 0 0.01 — 0.01 1.3749 -7 0.692 o-Xylene — 71.8 308 0.9982 0.36† 99 †† 0.1 0 — — — 1.3914 10 0.8802 0.93 100.0 210 — 144.41 210 4.0 2 — — — 1.3959 82 1.00 190 0.81 288 3.9 2 0.82 8.3 0.6 1.3772 53 — — — — — 1.4332 327 5.1 2 0.95 1.0 0.70 1.3284 52 —3————— 2.5 1 0.35 — 0.48 1.3689 -18 4.7 6 0.51 — — 1.3788 16 4.2 6 0.43 — — 1.3957 64 6.7 3 — — — 1.4700 187 — 2 — — — — 177 0.0 0 0.00 — 0.00 1.3575 -56 0.1 2 — — — 1.3650 -57 4.0 2 0.82 10.1 — 1.3856 74 6.1 6 — — — 1.4210 275 5.3 3 0.71 — ———— — 1.5102 68 ——— 4.0 3 0.45 3.7 0.53 1.4072 6 2.4 7 0.29 — 0.22 1.4969 40 — 7 — — — 1.5717 222 1.8 — — — — 1.4010 16 — — — — — 1.2850 N/A — — — — — 1.391 10.2 8 — — — 1.3330 N/A 2.5 7 0.26 — — 1.5054 63 40 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns ACE® Ultra-Inert, Base Deactivated HPLC and ACE® Excel UHPLC columns give you the choices HPLC/UHPLC Columns you need to achieve successful separations. 17 Phases C18 | C18-AR | C18-PFP | C18-Amide | SuperC18 | CN-ES | AQ | C8 | C4 | CN | Phenyl | Silica | C18-HL | NH2 | HILIC-A | HILIC-B| HILIC-N • Leverage 5 different mechanisms of separation offered by these bonded phases to achieve optimum selectivity for all peak pairs in your sample. 3 Pore Sizes 90 Å | 100 Å | 300 Å • Match the stationary phase support to the molecular size of your analytes. For peptides and proteins, choose a 300 Å pore. For molecules smaller than 5,000 daltons, choose 100 Å pore. Plus, optimize your column’s hydrophobic strength and loading capacity for your particular separation needs. The 90 Å pore C18-HL stationary phase offers greater surface area for higher loading capacity. 6 Particle Sizes 1.7 μm | 2 μm | 3 μm | 5 μm | 10 μm | 15 μm • Choose the right particle size for UHPLC, HPLC or preparative HPLC. 11 Standard Column Lengths 300 mm |250 mm | 200 mm | 150 mm | 125 mm |100 mm | 75 mm | 50 mm | 35 mm | 30 mm | 20 mm • Find the right balance between analysis time, resolution and peak capacity. Enquire for lengths not listed. 12 Standard Column IDs 0.075 mm | 0.10 mm | 0.30 mm | 0.50 mm | 1.0 mm | 2.1 mm | 3.0 mm | 4.0 mm | 4.6 mm | 10.0 mm | 21.2 mm | 30.0 mm • ACE: from capillary to preparative applications. ACE® Column Configurations • ACE 100 Å HPLC Columns • ACE Excel UHPLC Columns • ACE 300 Å Bioanalytical Columns • Capillary & Nano Columns • Preparative HPLC Columns • Guard Columns 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN A-1 LIFE SCIENCE

Colonnes HPLC/UHPLC Leverage the power of stationary phase selectivity with ACE® bonded phases. Colonnes HPLC/UHPLC Rs = N α-1 k 4 α 1+k Rs The resolution equation identifies the parameters that contribute to resolution: efficiency (N), retention (k) and selectivity (α). There has been a great deal of emphasis in the past several years on column efficiency and the use of ultra-efficient columns, so called “UHPLC” columns, as a means of achieving separation goals. UHPLC has proved its value by contributing to improvements in laboratory productivity through faster separations and faster method development. However, selectivity is often overlooked and its importance overshadowed by the emphasis on column efficiency. This is unfortunate. Of the three parameters that affect resolution, selectivity is the most powerful. By leveraging both efficiency and selectivity, better and faster separations often can be achieved. The effect of N, α and k on resolution (Rs) α N k Increasing N, α or k increases Resolution (Rs). However, as can be seen from these plots, increasing either N or k suffers from quickly diminishing returns. Increasing selectivity (α), on the other hand, does not have this problem and, therefore, becomes the most powerful of these three variables to optimize when developing a separation. In reversed-phase chromatography, there are multiple mechanisms of interaction between the stationary phase and analytes that can be leveraged to achieve a separation. These mechanisms of interaction include: hydrophobic binding, π–π, hydrogen bonding, dipole-dipole, and shape selectivity. Different types of bonded phases will offer one or more of these mechanisms of interaction. ACE reversed bonded phases are listed below and the principal mechanisms of interaction that are possible with each, depending on the analyte and the mobile phase conditions. Comparison of separation mechanisms/interactions offered by different bonded phases ACE Hydrophobic π–π Hydrogen Dipole- Shape Bonded Binding Bonding Dipole Selectivity Phase C18 Strong None None None Weak C18-HL Very strong None None None Weak C18-AR Strong Strong Moderate Moderate Moderate C18-PFP Strong Strong Strong Strong Strong AQ Moderate None Moderate Weak None C8 Moderate None None None None C4 Weak None None None None Phenyl Moderate Strong Weak Moderate Weak CN Weak None Weak Strong None Amino Weak None Strong Weak None A-2 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns Leverage bonded phase selectivity to achieve better separations. HPLC/UHPLC Columns ACE bonded phases provide a wide variety of powerful retention mechanisms to choose from when developing separations. By leveraging both efficiency and selectivity, much better separations can be achieved. Below illustrates the selectivity difference between two ACE Here is another illustration of the power of bonded-phase bonded phases, the C18-AR and the Phenyl. Although both selectivity. One separation was done on a UHPLC column bonded phases offer the possibility of moderate-strong π–π packed with a C18 bonded phase and the other separation and dipole-dipole interactions, they differ significantly in was done on a UHPLC column packed with a C18-PFP bonded the strength of other possible mechanisms of interaction, phase. The additional mechanisms of separation offered by the particularly hydrophobic binding. The additional, or different, C18-PFP bonded phase generates a better overall separation. mechanisms of separation that are possible with the C18-AR yield a better overall separation for this complex mixture. Comparison of Selectivity Differences Between C18-AR ACE Excel delivers excellent resolution and peak shape: and Phenyl Phases Pharmaceuticals and Related Compounds by UHPLC Column Dimensions: 50 x 2.1 mm, 3 μm 1. Metronidazole Conditions Sample Mobile vP/hva)pBpseHH=:222A0..=77m2iiMnn0mMHK2MHeOO2;PKHOH/42,HPO2O4, 2. 3-Hydroxybenzoic acid Column Dimensions: 50 x 2.1mm 1. Paracetamol 3. Phenol Mobile Phase: A = 5 mM formic acid in H2O 2. Hydrochlorothiazide 4. Benzyl alcohol B = 5 mM formic acid in MeOH 3. Methylphenylsulphoxide 5. Caffeine Gradient: 3 to 100% B in 5 minutes 4. Methylphenylsulphone (65:35, 6. Salicylic acid Flow Rate: 0.6 mL/min Flow rate: 0.6 mL/min 7. Quinoxaline Temperature: 40 °C Temperature: 60 °C 8. Benzoic acid Detection: UV 254 nm Detection: UV 214 nm 9. Quinine Gradient: 3 to 100% B in 5 min. 10. Phenacetin and hold for 1 min. 11. 1,4-Dinitrobenzene 12. 1,3,5-Trinitrobenzene 13. Furosemide 14. 1,3,5-Trimethoxybenzene 15. Piroxicam 16. Carvedilol 17. Ethyl benzoate 18. Nortriptyline ACE 3 C18-AR 56 Waters ACQUITY 2 Pmax = 581 bar 1.7 BEH C18 1 3 15 4 4 14 18 2 17 13 78 910 11 12 13 16 0 1 2 Time (sec) ACE 3 Phenyl 65 ACE Excel 2 C18-PFP 1 2 Pmax = 365 bar 15,17 3 4 24 12,13,14 18 3 87 10 9 11 16 1 The greater hydrophobicity of the C18-AR phase provides more retention 0 1 2 and better selectivity for the peak pairs (13, 14) and (15, 17). Also note the Time (sec) numerous elution order changes on the C18-AR versus the Phenyl phase. The chromatograms generated on the C18 UHPLC column and the C18-PFP column are equally fast. However, the C18-PFP column provides better selectivity for peak pairs (1, 2) and (3, 4) and, therefore, is able to provide a superior overall separation. Note: Comparative separations may not be representative of all applications. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN A-3 LIFE SCIENCE

Colonnes HPLC/UHPLC Colonnes HPLC/UHPLC ACE® Excel brings renowned ACE® HPLC performance to UHPLC It is important to recognize that column selectivity and column efficiency are independent variables and you do not have to choose to use one rather than the other when developing a separation. In fact, for the best overall separation you should optimize both, as well as retention. This is especially true when there is a need for a high speed separation. ACE Excel UHPLC columns are engineered to take full advantage of low dispersion, ultra-high pressure UHPLC instruments (up to 1000 bar) and are designed to be compatible with all systems. ACE Excel UHPLC columns deliver better peak shape for basic compounds, improved column-to-column reproducibility, additional stationary phases that leverage multiple mechanisms of separations, and improved column ruggedness. The availability of ACE Excel UHPLC columns means chromatographers now have more choices to achieve better outcomes with their UHPLC instruments. Utilizing both column efficiency and bonded phase selectivity to develop a ACE Excel columns provide high-speed, high-resolution separations faster separation Sample: Conditions Conditions: Compound: 1. Aspirin Mobile Phase: A = 5mM Formic acid Columns: ACE Excel C18, 2 µm, 3.0 x 50 mm 1. Acetanilide 2. Phenacetin 5. Nimesulide (0.189)ml/L); Mobile Phase: 30% B to 100% B in 60 seconds 2. Acetophenone 3. 1,3-dinitrobenzene 6. Ibuprofen B = 5mM Formic acid in MeOH A = Water + 0.1% TFA B = Acetonitrile 3. Propiophenone 4. Ethyl benzoate 7. Indomethacin Column Temperature: 40°C Flow Rate: 2.5 mL/min 4. Butyrophenone Detection: PDA, 254nm Column Temperature: 40 ºC 5. Benzophenone Column: ACE C18, 5μm, 4.6 x 100 mm Pressure: 703 bar (10,335 psi) 6. Valerophenone Flow rate: 1.0 mL/min 7 Detection: PDA, 254 nm 7. Hexanophenone tG = 30.0 mins Instrument: Agilent 1290 UHPLC 8. Heptanophenone Injection Volume: 3µL 9. Octanophenone 5 2 3 5 2 1 46 1 25 minutes 3 4 67 89 Column: ACE Excel C18, 2μm, 2.1 x 50 mm 7 0 12 24 36 48 60 Flow rate: 0.6 mL/min 2 5 Time (sec) 46 tG = 5.0 mins Chromatographic data supplied courtesy of a west coast U.S. pharmaceutical company. Injection Volume: 1µL 3 An ACE Excel C18 column provides baseline separation of these 9 analytes in less than 60 seconds. 1 4.5 minutes Column: ACE Excel C18-PFP, 2 μm, 2.1 x 30 mm 7 Flow rate: 2.5 mL/min 2 tG = 0.7 mins Injection Volume: 1µL 3 5 1 46 45 seconds By utilizing the higher efficiency of a UHPLC column, a shorter length column run at higher mobile phase linear velocity can be used to reduce the separation time of this mixture by 80% compared to the HPLC column. However, by also optimizing bonded phase selectivity, in this case using the enhanced selectivity of a C18-PFP bonded phase, separation time can be reduced even further. The ACE Excel C18-PFP UHPLC column reduced separation time of this mixture by over 80% compared to the C18 UHPLC column and by 96% compared to the C18 HPLC column, while maintaining excellent resolution of all peaks. A-4 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns ACE® Excel™ UHPLC columns HPLC/UHPLC Columns ACE Excel UHPLC columns: • Fully compatible with all commercial UHPLC instruments • Brings renowned ACE HPLC columns’ advantages to UHPLC columns • Provides users of UHPLC with additional choices in stationary phases, including the powerful C18-AR and C18-PFP phases • Delivers a high level of reliability and ruggedness • Offers easy scalability from UHPLC to HPLC to preparative LC • Available in 1.7, 2, 3 and 5 µm particle sizes for UHPLC Manufacturing UHPLC columns is tough and there is a general perception that they are not as rugged and reliable as HPLC Columns. By drawing on their vast experience in manufacturing the finest HPLC columns, Advanced Chromatography Technologies is able to produce very reliable UHPLC columns. Now, chromatographers will get even more from their UHPLC instruments. Excellent peak shape for basic compounds, improved column-to-column reproducibility, additional stationary phases that leverage multiple mechanisms of separations, and improved column ruggedness are now available to UHPLC users with ACE Excel UHPLC columns. ACE Excel delivers excellent resolution and peak shape Conditions: Analytes: 6. Phenacetin 11. Ibuprofen Mobile Phase: A = 5 mM formic acid in H2O and B = 5 mM 1. Paracetamol 7. 1,3-Dinitrobenzene 12. Indomethacin formic acid in MeOH 2. Hydrochlorothiazide 8. 1,2,4-Trimethoxybenzene 13. Mefenamic acid Gradient: 3 to 100% B in 5 minutes 3. Methylphenylsulphoxide 9. Ethyl benzoate Flow Rate: 0.6 ml/min 4. Methylphenylsulphone 10. Nimesulide Temperature: 40 °C 5. Aspirin Detection: UV, 254 nm Column Dimensions: 50 x 2.1mm Waters ACQUITY 1.7 BEH C18 Phenomenex Kinetex 1.7 C18 1,2 21 7 12 Pmax = 581 bar Pmax = 540 bar 36 13 7 12 4 58 10 13 5 6 9 11 3 6 10 4 58 9 11 4 0 1 2 3 5 6 0 1 2 3 4 ACE Excel 2 C18-PFP ZORBAX Eclipse 1.8 XDB C18 7 12 21 3 7 12 Pmax = 365 bar 12 Pmax = 540 bar 4 10 13 10 13 6 8 9 11 36 5 45 9 11 8 0 1 2 3 4 5 6 0 1 2 3 4 5 6 Time (min) Time (min) The C18 bonded phases shown in these comparison chromatograms are not able to fully separate all 13 analytes. The ACE Excel C18-PFP, due to its extra separation mechanisms, is able to baseline separate all analytes. Note: Comparative separations may not be representative of all applications. 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN A-5 LIFE SCIENCE

Colonnes HPLC/UHPLCColonnes HPLC/UHPLC ACE® Excel columns are very forgiving and provide the same durability and lifetime that you would expect from HPLC columns. ACE Excel will change your perception about the durability of UHPLC columns. Not only are the ACE Excel columns packed better, they incorporate a proprietary inlet frit design that is much less prone to plugging than other UHPLC columns. It is still recommended that you filter samples and mobile phases as you would with any UHPLC column, but ACE Excel columns are much more forgiving than other UHPLC columns and provide the same durability and lifetime that you would expect from HPLC columns. ACE® columns have a well-deserved reputation for reproducibility. Column-to-column reproducibility is affected by the production of the silica stationary phase support, the bonding of the stationary phase to the stationary phase support, and the packing of the stationary phase into the column. The better each stage of the column manufacturing process is controlled, the better will be the reproducibility and the quality of the column. ACE columns are subjected to extensive controls at each stage of the column manufacturing process. These controls ensure that ACE columns produce reliable, predictable performance from column-to-column and lot-to-lot. To the right provides an example of a typical Batch Validation test. To request a batch validation certificate please contact your sales representative. Subtle changes in silanol activity are one of the primary causes of column-to-column selectivity changes. ACE and ACE Excel columns have excellent reproducibility due to the use of ultra pure reagents and strict control of the manufacturing process that results in a high purity silica stationary phase support with uniform surface characteristics. Combining this high purity silica with advanced bonding techniques results in a family of highly inert phases that provide an outstanding level of column-to-column reproducibility by virtually eliminating silanol interference in the chromatography. ACE Excel columns have a well deserved reputation for durability Initial injection at 1000 bar 0 1 2 3 4 5 6 7 After 2000 runs 0 1 2 3 4 5 6 7 Time (min) A 2.1 x 100 mm ACE Excel C18 UHPLC column was subjected to over 2,000 gradient runs at an average pressure of 1,000 bar (14,500 psi). The column efficiency (plate number) and retention times were essentially unchanged at the end of this stability study. A-6 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE

HPLC/UHPLC Columns HPLC/UHPLC Columns ACE® Guard Columns (Not suitable for ACE Excel UHPLC columns) ACE® Integral Guard Columns • Guard column is incorporated into the analytical column’s inlet end-fitting • Ultra low dead volume design provides protection without degrading performance • Easy to change cartridge design ACE analytical columns are manufactured with state-of-the-art column fittings that incorporate the guard column as an integral part of the analytical column. To install a guard column on an ACE analytical column, simply replace the standard column inlet end- fitting with the end-fitting designed as a guard column holder. Then, insert a guard cartridge packed with the desired stationary phase into the guard column holder (See photograph). Don’t worry about disturbing the packing bed when installing the guard column holder. It is well protected by a PEEK frit cap, even with the column end-fitting removed. The integral guard column system is available for columns with internal diameters of 2.1, 3.0, and 4.6 mm. For preparative columns (> 10 mm), a typical stand-alone style of guard column is available, packed with the appropriate stationary phase. Stand-alone style guard column holders are also available for situations where they are preferred or required. A column coupler (p/n ACE-C0001), or connection tubing and fittings, will be required to use this style of guard column. Using a guard column to protect your analytical column can substantially increase the column lifetime and improve the quality of your chromatography. But, to be effective, the guard column must be replaced often enough to prevent contaminants from saturating the guard column and bleeding through to the analytical column. The best way to determine the optimum time to replace a guard column for a specific set of sample and mobile phase conditions is through experience. However, it is helpful to have some quantitative measure to help make the replacement decision. By monitoring plate number (N), pressure (P), and resolution (Rs), the performance of the guard and analytical column can be closely watched to determine when a guard column should be replaced. We suggest replacing the guard column when any one of these parameters changes by more than 10%. Column with no guard Integral guard column systems 1 888-226-2775 :: [email protected] :: www.lifescience.ca CANADIAN A-7 LIFE SCIENCE

Mobile Phase: A: 0.1M CH 3 COONH 4 3. Simazine B: MeCN 4. Cyanazine Colonnes HPLC/UHPLC 5. Atrazine Flow Rate: 0.3 mL/min 6. Internal standard 7. Sebuthylazine Gradient: T(mins) %A %B 8. Propazine 9. Terbuthylazine 0 90 10 10. Prometryn 11. Terbutryn 40 20 80 12. Alachlor 13. Pendimethalin 47 10 90 49 90 10 Temperature: 40° C ACE® and ACE Excel™ C18 Detection: UV, 220nm (pendimethalin at 245nm) Injection Volume: 25µl Colonnes HPLC/UHPLC Sample: 0.05 µg/l standards in 10:90 MeCN/H2O Physical Properties 35 Pesticides in Water Silica : Ultra-Inert Base Deactivated Particle Size : 1.7 µm, 2 µm, 3 µm, 5 µm 30 Surface Area : 300 m²/g 25 6 20 Pore Size : 100 Å 15 Bonded Phase : Octadecyl groups 10 1 End-Capping : Yes 2 3 5 45 0 78 9 10 11 Carbon Load : 15.5% -5 USP Code : L1 12 13 pH Range : 2.0-8.0 Mechanisms of Separation Strength of Interaction 0 10 15 20 25 30 35 40 Hydrophobic binding Strong Time (min) interactions Weak Shape selectivity Reproduced with kind permission of Amt der Tiroler Landesregierung, Chemisch-technische Umweltschutzanstalt, Innsbruck, Austria. Conditions: Compounds: Column: ACE 3 C18, 150 x 2.1mm 1. Desisopropylatrazine Part Number: ACE-111-1502 2. Desethylatrazine Mobile Phase: A: CH3COONH4 in H2O 3. Simazine B: MeCN 4. Cyanazine Target Analytes Flow Rate: 0.3 mL/min 5. Atrazine • Analytes differing in hydrophobicity Gradient: T(mins) %A %B 6. Internal standard • Polar, moderately polar, and non-polar analytes 0 90 10 7. Sebuthylazine • Uncharged acids and bases 40 20 80 8. Propazine • Ionized acids or bases using ion-pairing 47 10 90 9. Terbuthylazine 49 90 10 10. Prometryn Recommended Applications Temperature: 40° C 11. Terbutryn • Analytes differing in hydrophobicity Detection: UV, 220nm (pendimethalin at 245nm) 12. Alachlor • Homologous compounds differing by –CH2 Injection Volume: 25μl 13. Pendimethalin Sample: 0.05 μg/l standards in 10:90 MeCN/H2O Antihistamines and Expectorants Conditions: Compounds: Column: ACE 5 C18, 150 x 4.6mm 1. Maleic acid Part Number: ACE-121-1546 2. Salicylamide Mobile Phase: 45:55 MeOH/20mM KH2PO4 (pH 3.0) 3. Guaifenesin Flow Rate: 1.0 mL/min 4. Guaiacol Temperature: Ambient 5. Chlorpheniramine maleate Detection: UV, 220nm 6. Dextromethorphan Injection Volume: 0.4µl 240 220 200 180 2 Response–MilliVolts 160 140 1 3 120 4 100 80 60 56 40 20 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Time (min) A-8 CANADIAN 1 888-226-2775 :: [email protected] :: www.lifescience.ca LIFE SCIENCE